Lag0034848 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034848
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cyanogenic beta-glucosidase-like
Location	chr3: 11592890 .. 11594497 (+)
RNA-Seq Expression	Lag0034848
Synteny	Lag0034848

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCCTTCTTGTTGTGAATTGTGCATCTGAGGAATGTGAAGCTGCTCTAGTGAGTTCAATGGAATAGTGTCACTGTGTTTGTGTATTTGTTGCTGACTGCTTCTTCTCCTCTATTCCACAAGCTGTATTATTTGAACTATTGTACCTTGCTGTTGATTCATGGTTGAATAAGAAAGGACAGAGCACCATAGTGGATGTAGATCACATTGATCGAACCACTTTAAAACCTTGTGTCTATTTTCTCTTCTATGTTCTCTGTTTTCAGTTTGATGCATTATTTCTGTGTTGCTTGGGTGTGTTGCTGTGGTTCAGCATTTCTGTGATAACCTGTTATGTGTGTTCTTTGTGAATTGAGTGGCCAATATCTGTAGTTTATCTCAACAATATAATATTCATTAAATTTAAGATAGTCATTTTCTCTATTGAATCTAACAATTTTGGTATTTATATGTTAATAATGTTGCAGGTGAATGCATCATCTTGGCTTTCAGTTTATCCACATGGATTGAAACATGTGTTAATAGACATGAAAAACAATTATAAGAATCCACTTATCTGCATCACAGAAAATGGTATTAAAAAATATATATATATATATTTTTTATATATCAAGAAAAAGAGTAATCACCAGAATTAATTACCCTTATTATTTACAAGTCTGAATTGTACACTGTGCAGGATATTTTGACTATAATGATCCTAATGTTCAAAAACTACTTTGCGATCGGGAAAGAGTTCATTACTATATAGAACATCTCTATTATCTTCACCAACCAATCAAGTAAGTAACTCCTTGTTTCATAATCATTTGGTTTTTGGATTTTGAAAATTAAACCTATCAACGCTATCATCATCCATAAGTTTATTTGTTTTGTCACCTACCTTCTTCCCATATTTTCAAAAGCTAAGTCAAGTTTTTGTTGGGAGTGCGACTAAAGTCTCACATTGGTTAGACAAGGGGATAATCATAGATATATAAGGAAGGATAAGTATCTCCATTGGTACGAGACCTTTTGGGGTGGACTAAAAAACAAAACCACGAGGGCTTATGCCCAAAGTTGACAATATCATACCATTGTAGAGATGTGTGTGGGTGTTTGATGTCCCCTTTCAAGTGGTATCAGAGTCATGCCCCAAACGTAGCCATGTCATGGCTTTGCTTTTATGGTTCACCCAAAAAAAAATAGTTTTCAAAAACTTGTTTTTCTTTTTGGAATTTGGCTAAGAATTCAAATGTGTGCATAACAAAGATGAAACCATGGTAAGAAAATGAGGAGAAAACAAGCATAATTTTCAAAAACAAAAAATCAAATGGTTATCAAACGGCCCTTAGGAAATTTTTGACTTAAAATAGTTGTTTGGTAACATATATGTTTTTTTTTTTTTTTTTTGGTTGCATATGTTCTTAATATGATTCTTTTGGAAACCTTATTATAGGGTTGGTGTAAGGGTAGAGGGATTCTTTGCATGGTCACTTTTGGACACATTTGAATGGCAAGATGGATATACCAAGCGCTTTGGCCTTATCTACATTGATTTCAAGAACAACTTGAATAGAATTCCAAAGAAAACACTAAAATGGTTTTCTAGGTTCCTCAAGACTTGA

mRNA sequence

ATGATCCTTCTTGTTGTGAATTGTGCATCTGAGGAATGTGAAGCTGCTCTAGTGAATGCATCATCTTGGCTTTCAGTTTATCCACATGGATTGAAACATGTGTTAATAGACATGAAAAACAATTATAAGAATCCACTTATCTGCATCACAGAAAATGGATATTTTGACTATAATGATCCTAATGTTCAAAAACTACTTTGCGATCGGGAAAGAGTTCATTACTATATAGAACATCTCTATTATCTTCACCAACCAATCAAGGTTGGTGTAAGGGTAGAGGGATTCTTTGCATGGTCACTTTTGGACACATTTGAATGGCAAGATGGATATACCAAGCGCTTTGGCCTTATCTACATTGATTTCAAGAACAACTTGAATAGAATTCCAAAGAAAACACTAAAATGGTTTTCTAGGTTCCTCAAGACTTGA

Coding sequence (CDS)

ATGATCCTTCTTGTTGTGAATTGTGCATCTGAGGAATGTGAAGCTGCTCTAGTGAATGCATCATCTTGGCTTTCAGTTTATCCACATGGATTGAAACATGTGTTAATAGACATGAAAAACAATTATAAGAATCCACTTATCTGCATCACAGAAAATGGATATTTTGACTATAATGATCCTAATGTTCAAAAACTACTTTGCGATCGGGAAAGAGTTCATTACTATATAGAACATCTCTATTATCTTCACCAACCAATCAAGGTTGGTGTAAGGGTAGAGGGATTCTTTGCATGGTCACTTTTGGACACATTTGAATGGCAAGATGGATATACCAAGCGCTTTGGCCTTATCTACATTGATTTCAAGAACAACTTGAATAGAATTCCAAAGAAAACACTAAAATGGTTTTCTAGGTTCCTCAAGACTTGA

Protein sequence

MILLVVNCASEECEAALVNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIEHLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFSRFLKT

Homology

[Close]

BLAST of Lag0034848 vs. NCBI nr
Match: XP_022988084.1 (cyanogenic beta-glucosidase-like [Cucurbita maxima])

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-46
Identity = 87/124 (70.16%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 18  VNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIE 77
           VNASSWLS+YP GLK +LI MKN+Y NP I ITENG+FDY+DP+ +KLLCDR+R  Y++ 
Sbjct: 376 VNASSWLSIYPEGLKKLLITMKNDYNNPSIYITENGFFDYDDPSARKLLCDRKRTRYHMG 435

Query: 78  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 137
           HLY+LH+ +  GVRV G+FAWSLLDTFEW DGYTKRFGL+YID  NNL R PK +LKWFS
Sbjct: 436 HLYFLHEAMMDGVRVNGYFAWSLLDTFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFS 495

Query: 138 RFLK 142
            FLK
Sbjct: 496 YFLK 499

[Close]

BLAST of Lag0034848 vs. NCBI nr
Match: XP_023516697.1 (beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 191.4 bits (485), Expect = 5.2e-45
Identity = 86/124 (69.35%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 18  VNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIE 77
           VNASSWLSVYP GL+ +LI MKN+Y NP I ITENG+FDY+DP+ +KLLCDR+R  Y++ 
Sbjct: 376 VNASSWLSVYPKGLQKLLITMKNDYNNPSIYITENGFFDYDDPSARKLLCDRKRTFYHMG 435

Query: 78  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 137
           HLY+L + ++ GVRV G+FAWSLLDTFEW DGYTKRFGL+YID  NNL R PK +LKWFS
Sbjct: 436 HLYFLLEAMRDGVRVNGYFAWSLLDTFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFS 495

Query: 138 RFLK 142
            FLK
Sbjct: 496 YFLK 499

[Close]

BLAST of Lag0034848 vs. NCBI nr
Match: XP_022960679.1 (beta-glucosidase 12-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 191.0 bits (484), Expect = 6.8e-45
Identity = 85/124 (68.55%), Postives = 102/124 (82.26%), Query Frame = 0


Query: 18  VNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIE 77
           VNASSWLSVYP GL+ +LI MKN+Y NP I ITENG+FDY+DP+ +KLLCDR+R+ Y++ 
Sbjct: 376 VNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFFDYDDPSARKLLCDRKRIFYHMG 435

Query: 78  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 137
           HLY+L Q ++ GVRV G+FAWS LD FEW DGYTKRFGL+YID  NNL R PK +LKWFS
Sbjct: 436 HLYFLRQAMRDGVRVNGYFAWSFLDNFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFS 495

Query: 138 RFLK 142
            FLK
Sbjct: 496 LFLK 499

[Close]

BLAST of Lag0034848 vs. NCBI nr
Match: XP_022960680.1 (beta-glucosidase 12-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 191.0 bits (484), Expect = 6.8e-45
Identity = 85/124 (68.55%), Postives = 102/124 (82.26%), Query Frame = 0


Query: 18  VNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIE 77
           VNASSWLSVYP GL+ +LI MKN+Y NP I ITENG+FDY+DP+ +KLLCDR+R+ Y++ 
Sbjct: 279 VNASSWLSVYPKGLQKLLITMKNDYDNPSIYITENGFFDYDDPSARKLLCDRKRIFYHMG 338

Query: 78  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 137
           HLY+L Q ++ GVRV G+FAWS LD FEW DGYTKRFGL+YID  NNL R PK +LKWFS
Sbjct: 339 HLYFLRQAMRDGVRVNGYFAWSFLDNFEWSDGYTKRFGLVYIDRNNNLTRTPKDSLKWFS 398

Query: 138 RFLK 142
            FLK
Sbjct: 399 LFLK 402

[Close]

BLAST of Lag0034848 vs. NCBI nr
Match: XP_038880765.1 (cyanogenic beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 187.6 bits (475), Expect = 7.5e-44
Identity = 85/125 (68.00%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 18  VNASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIE 77
           +NASSWLSVYP GL+ +L+ MKNNY NP I ITENG+FDY+DPNV KL+CD++R  Y+I 
Sbjct: 378 LNASSWLSVYPPGLEKLLVTMKNNYNNPNIYITENGFFDYDDPNVGKLICDKKRTLYHIT 437

Query: 78  HLYYLHQPIKV-GVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWF 137
           HLY+LH+ I   GV+V+G+FAWSLLDTFEW DGYTKRFGL+YID  NNL R PKK+ +WF
Sbjct: 438 HLYHLHRAIFTHGVQVKGYFAWSLLDTFEWSDGYTKRFGLVYIDRSNNLKRTPKKSQEWF 497

Query: 138 SRFLK 142
           + FLK
Sbjct: 498 TYFLK 502

[Close]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot
Match: Q5Z9Z0 (Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 56/122 (45.90%), Postives = 83/122 (68.03%), Query Frame = 0


Query: 21  SSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPN--VQKLLCDRERVHYYIEH 80
           SSWL +YP G++ +L+  K  Y NP I ITENG  + N+ N  +++ L D  R+ +Y +H
Sbjct: 381 SSWLYIYPKGIEELLLYTKRTYNNPTIYITENGVDEVNNENLSLKEALIDTTRIEFYRQH 440

Query: 81  LYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFSR 140
           L+++ + ++ GV V G+FAWSL D FEW DGY+ RFG+ YID+K+ L R PK++ +W   
Sbjct: 441 LFHVQRALRQGVDVRGYFAWSLFDNFEWMDGYSVRFGINYIDYKDGLKRYPKRSSQWLQN 500

[Close]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot
Match: Q7F9K4 (Beta-glucosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU10 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.6e-28
Identity = 56/124 (45.16%), Postives = 82/124 (66.13%), Query Frame = 0


Query: 20  ASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDP--NVQKLLCDRERVHYYIE 79
           AS W  +YP GL+ +L+ +K NY NP I ITENG  + N+    +++ L D  R+ YY +
Sbjct: 409 ASFWFYIYPEGLRELLLHIKENYGNPTIYITENGVDEINNKTMRLKEALKDDIRIEYYHK 468

Query: 80  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 139
           HL  L   ++ G  V+G+FAWSLLD FEW +GYT RFG+ ++D+ N + R PK + +WF 
Sbjct: 469 HLLALLSAMRDGANVKGYFAWSLLDNFEWSEGYTVRFGINFVDYDNGMKRYPKNSARWFK 528

Query: 140 RFLK 142
           +FL+
Sbjct: 529 KFLR 532

[Close]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot
Match: O64879 (Beta-glucosidase 15 OS=Arabidopsis thaliana OX=3702 GN=BGLU15 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.7e-28
Identity = 56/121 (46.28%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 20  ASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPNVQKLLCDRERVHYYIEHL 79
           AS WL +YP G++ +++  K  +K+P++ ITENG  +++   +   L D +R+ YY  HL
Sbjct: 382 ASDWLLIYPKGIRDLVLYAKYKFKDPVMYITENGRDEFSTNKI--FLKDGDRIDYYARHL 441

Query: 80  YYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFSRF 139
             +   I VG  V+GFFAWSLLD FEW  GYT RFGL+Y+DFK+   R PKK+ +WF + 
Sbjct: 442 EMVQDAISVGANVKGFFAWSLLDNFEWAMGYTVRFGLVYVDFKDGCKRYPKKSAEWFRKL 500

Query: 140 L 141
           L
Sbjct: 502 L 500

[Close]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot
Match: Q9FIW4 (Beta-glucosidase 42 OS=Arabidopsis thaliana OX=3702 GN=BGLU42 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.7e-28
Identity = 60/124 (48.39%), Postives = 80/124 (64.52%), Query Frame = 0


Query: 20  ASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYND--PNVQKLLCDRERVHYYIE 79
           AS WL   P G++  L  M   Y +P I ITENG  D +D   ++  +L D+ RV Y+  
Sbjct: 357 ASDWLYAVPWGIRKTLNYMSKKYNHPPIFITENGMDDEDDGSASIHDMLDDKRRVDYFKS 416

Query: 80  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 139
           +L  + Q I+ GV ++G+FAWSLLD FEW  GYTKRFGL+Y+D+KN L R PK +  WF 
Sbjct: 417 YLANVSQAIEDGVDIKGYFAWSLLDNFEWAQGYTKRFGLVYVDYKNGLTRHPKSSAYWFM 476

Query: 140 RFLK 142
           +FLK
Sbjct: 477 KFLK 480

[Close]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.5e-28
Identity = 57/123 (46.34%), Postives = 80/123 (65.04%), Query Frame = 0


Query: 20  ASSWLSVYPHGLKHVLIDMKNNYKNPLICITENGYFDYNDPN--VQKLLCDRERVHYYIE 79
           AS WL VYP G + +L+ +K NY NP + ITENG  ++N+    +Q+ L D  R+ YY +
Sbjct: 386 ASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKTLPLQEALKDDARIEYYHK 445

Query: 80  HLYYLHQPIKVGVRVEGFFAWSLLDTFEWQDGYTKRFGLIYIDFKNNLNRIPKKTLKWFS 139
           HL  L   I+ G  V+G+FAWSLLD FEW +GYT RFG+ ++D+ +   R PK +  WF 
Sbjct: 446 HLLSLLSAIRDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVDYNDGRKRYPKNSAHWFK 505

Query: 140 RFL 141
           +FL
Sbjct: 506 KFL 508

The following BLAST results are available for this feature:

BLAST of Lag0034848 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988084.1	1.6e-46	70.16	cyanogenic beta-glucosidase-like [Cucurbita maxima]	[more]
XP_023516697.1	5.2e-45	69.35	beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022960679.1	6.8e-45	68.55	beta-glucosidase 12-like isoform X1 [Cucurbita moschata]	[more]
XP_022960680.1	6.8e-45	68.55	beta-glucosidase 12-like isoform X2 [Cucurbita moschata]	[more]
XP_038880765.1	7.5e-44	68.00	cyanogenic beta-glucosidase-like [Benincasa hispida]	[more]

BLAST of Lag0034848 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z9Z0	1.9e-29	45.90	Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1	[more]
Q7F9K4	1.6e-28	45.16	Beta-glucosidase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU10 PE=2 SV=1	[more]
O64879	2.7e-28	46.28	Beta-glucosidase 15 OS=Arabidopsis thaliana OX=3702 GN=BGLU15 PE=1 SV=1	[more]
Q9FIW4	2.7e-28	48.39	Beta-glucosidase 42 OS=Arabidopsis thaliana OX=3702 GN=BGLU42 PE=2 SV=1	[more]
B8AVF0	3.5e-28	46.34	Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001360	Glycoside hydrolase family 1	PRINTS	PR00131	GLHYDRLASE1	coord: 113..125 score: 46.15 coord: 89..106 score: 52.89 coord: 68..79 score: 39.44 coord: 47..55 score: 54.22
IPR001360	Glycoside hydrolase family 1	PFAM	PF00232	Glyco_hydro_1	coord: 20..141 e-value: 1.3E-34 score: 119.4
IPR001360	Glycoside hydrolase family 1	PANTHER	PTHR10353	GLYCOSYL HYDROLASE	coord: 18..141
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 4..141 e-value: 4.9E-45 score: 155.7
None	No IPR available	PANTHER	PTHR10353:SF272	SUBFAMILY NOT NAMED	coord: 18..141
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 20..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034848.1	Lag0034848.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds