Lag0034564 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034564
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ulp1-like peptidase
Location	chr3: 8450595 .. 8454131 (-)
RNA-Seq Expression	Lag0034564
Synteny	Lag0034564

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCGAGGCCGACCTCGTCCTCCTAGGGTCTTTAAGAATTCGGAGGTGTTTTGGGATGAACCAAGCGGAACCGGGGCGATTTGGGGCATCACGGGTCGAAAGGAGGTGACCGAGCTCGGCCTAGACCATATGGGTCGGGCCAAGTCTTCCTATGAGTCGGACTTCTGGTCCTACCTTTGTCCGATTGTCCTTGTCAGCTCCTTATTTGTCAGTGCGATCCGAAATTACCCATAACACCTACCTCAATATCTGTTCAACTCAAAAATAACTTACGTGTATTTATTATTTAAGATATGTTTTTAACTCCTATACTAAAAGAAACAAATAATTTGATATATTTATTTGTATGTAAAATATTTGTAATCATGAAAGTGTGATGATGTACATTTTACTACATACAGATAAAAGATGGGTTGAAACTTATTTCAATTACATTTTTTTTTGGGTAAGAAAACCATTCTAGAAAAAATATATACAAGGTTAAATTACAAATTTAGTATTTAAACTTTGGTTGTGTTGAGAAAATTTTAAACTTTAAAGAGAGTGTTGTTTCTAAACTTTTAATATATAGATCTTAAAACTTTAAGAAATACTAAAATAACTTTTTTATTAATATTCATATCCTAAATTTGTTTGGTTGGATGGTCATAATTTTCAATTTTGTGTTTATTGACCAATTCAACTTTTGTTTAGATTCCTTGAACTACTATGCATAAAAGTTGAGAGTTTACGGTTCTATTAAACATAAAATTCAATTTACCTATTAGATCCATTGCTTTAATTTTTTTTTAATATGTCAAGGACCTATTAGAAAAAAATCAAAATTTCAAAGTCTCATCTAATGCATGTTGGGAAGTGGTTTTGAAATAACTTTTGTTATGTTCGAAATCACCCTATAACATAACTTTAATTATTCAAAATCAATTTTATGAGTTTGATTATTTACACGTTTAAACGTGATTTTTATGTCTTCAAACTTGATTTAGAATTATTAAAATTGCAATAGATATACAAAATTAAAAGTTTCTACAATCTATTAGACTCTCCTTTCAAACTCTAGAGTCTAGACATAACACTAAAACTTTAGACTTATTATTATTTTTATTTTTTAAATGAAAAAAAAAATTATTGACTTATTAAACATTTTTAGACATTTATTAAAAGGGCAATTGAGAATCATGGGCCCATTTAAATTTTGTTTTGTAAAAATGGGCCCAATTTTGAATTTACTTATTTGGGGACCCCTGACATTGTGAAAAGACCAAATTATCCTTGCCCTAAACACTCTATCTCTCTGCTCGACCGTTGCTTCCTTCTTCTTCTTCCTCAGACTTTCCTTCTCTCACGACTTCTCCATTTCTTCCTCACATTCGTCTGCTGTTTCCTTGGAAATATTTCGCGGAAATCAAGCTTACTTCTACACGAGGTAAGTAACTATTATCATTTTTGGGTTTTCATCGCGCGATTTTATCCTTCGGCAATGTCATTTTTGGGTTTTGTTAGAAATCTAGGGTTTTGCTGTATTGGATGGATTCTGATTATGTTTTAGGTCTTAGTTAAGTTCGTCGGCGGTTTCTGAGGAGGGTAGAGGGAAGAAGAACAGGATTTTGTGCTGTTTAAGACAACGTTTCTGGAATCGAGATGAATCCCGGCCGGGATTCATCTTCTGTAATTTCTCGGTCAACCGGGAAATTTGGCAGAACTCGAAGCCTTTCTCGGTTGACCGAGTTACACAGACCAAATCTCGCTCTCGATTTCGACCGAGATTTAGCCGGGAAATTTGGCAGAACTCGACCCTTTGGTTGACCGAGAATTATAGAAGTTGAATCCCGGTCACGATTTCGACCGAGATTTTGACCGGGACTGGCGAGGTTCGTTTTTAGTTAATTTTTTTTTAACATTGTATTCTTGAAAATGTTGATTATGAATTGTTGTAATTTGCTAGGAGTATGGAAATCACCGAGAAAATCCCCCTTGCTGACCATTTTCCGGCTGCGTTGACATGTTGCTCACACCTAAACAAAACCATTAGCAATATTAAGTCAACCCTAAGTCGTACCCAATTAGACCTCTTTAGGCAAACATGTTTGGGCCTTTAATAGATGTGAATGTTATTTTTAATGGCCAATTAGTACACTATATCCTCCTTAGGGAAGTAGAGGAGAATAGGGCAAATGTGATGAGTTTTAAATTGTTAGGTCAGAAGGTCTCCTTTGGTAAGAGAGATTTTGACCTCATAACCGGCCTTCGTCATTCATTTAGACCAATGAGGAGAGATAGAGAGGGCCCTCCCAATAGACTCCTAAGATTATATTTTAGAGAGAATGTAGGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACTCTTCAGTTTGAGAACGACGAAGATGCAGTTAAGATCGCAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCTAGCACCTTAGACTTAATGGATGATTGGGTGGCATTCTGCAACGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGTTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATATGGTTTCCCGTTTGCTTTTCAGGTTAGTAGATAATTAAACTTTTGTAATGTAATCTTCATATAGTTTGTATGGTTTCCCAATTTTTTTCTTCAATTGTTTCTTTACACAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGTAAGTGAGACAGCCATCCCACGCATCCGTCGATGGTCTTGCGCCCACTCCCCATCATACACGATCATTGCTGATGAAGTTTTTGGATCCCGGGCGGTTAGTTATTATAATTATTTAAATGTTTTGTCTTAATTATTATTTGTAAACAACCAACCACTAACTAAGTAATTTTTTTGCAGGCTAGAGTTAGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGACCATGAGAACGACACGTTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACAGCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTCGAAACAGGCGAATTTGTGTTGACAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCCAAGCAGGTGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGACAAGCCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTCTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAG

mRNA sequence

ATGGGCCGAGGCCGACCTCGTCCTCCTAGGGTCTTTAAGAATTCGGAGGTGTTTTGGGATGAACCAAGCGGAACCGGGGCGATTTGGGGCATCACGGGTCGAAAGGAGGTGACCGAGCTCGGCCTAGACCATATGGGTCGGGCCAAGTCTTCCTATGAGTCGGACTTCTGGTCCTACCTTTGTCCGATTGTCCTTGTCAGCTCCTTATTTACTTTCCTTCTCTCACGACTTCTCCATTTCTTCCTCACATTCGTCTGCTGTTTCCTTGGAAATATTTCGCGGAAATCAAGCTTACTTCTACACGAGTTAAGTTCGTCGGCGGTTTCTGAGGAGGGTAGAGGGAAGAAGAACAGGATTTTGTGCTGTTTAAGACAACGTTTCTGGAATCGAGATGAATCCCGGCCGGGATTCATCTTCTGTAATTTCTCGGTCAACCGGGAAATTTGGCAGAACTCGAAGCCTTTCTCGGTTGACCGAGTTACACAGACCAAATCTCGCTCTCGATTTCGACCGAGATTTAGCCGGGAAATTTGGCAGAACTCGACCCTTTGGTTGACCGAGAATTATAGAAGTCAGAAGGTCTCCTTTGGTAAGAGAGATTTTGACCTCATAACCGGCCTTCGTCATTCATTTAGACCAATGAGGAGAGATAGAGAGGGCCCTCCCAATAGACTCCTAAGATTATATTTTAGAGAGAATGTAGGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACTCTTCAGTTTGAGAACGACGAAGATGCAGTTAAGATCGCAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCTAGCACCTTAGACTTAATGGATGATTGGGTGGCATTCTGCAACGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGTTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATATGGTTTCCCGTTTGCTTTTCAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGTAAGTGAGACAGCCATCCCACGCATCCGTCGATGGTCTTGCGCCCACTCCCCATCATACACGATCATTGCTGATGAAGTTTTTGGATCCCGGGCGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGACCATGAGAACGACACGTTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACAGCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTCGAAACAGGCGAATTTGTGTTGACAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCCAAGCAGGTGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGACAAGCCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTCTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAG

Coding sequence (CDS)

ATGGGCCGAGGCCGACCTCGTCCTCCTAGGGTCTTTAAGAATTCGGAGGTGTTTTGGGATGAACCAAGCGGAACCGGGGCGATTTGGGGCATCACGGGTCGAAAGGAGGTGACCGAGCTCGGCCTAGACCATATGGGTCGGGCCAAGTCTTCCTATGAGTCGGACTTCTGGTCCTACCTTTGTCCGATTGTCCTTGTCAGCTCCTTATTTACTTTCCTTCTCTCACGACTTCTCCATTTCTTCCTCACATTCGTCTGCTGTTTCCTTGGAAATATTTCGCGGAAATCAAGCTTACTTCTACACGAGTTAAGTTCGTCGGCGGTTTCTGAGGAGGGTAGAGGGAAGAAGAACAGGATTTTGTGCTGTTTAAGACAACGTTTCTGGAATCGAGATGAATCCCGGCCGGGATTCATCTTCTGTAATTTCTCGGTCAACCGGGAAATTTGGCAGAACTCGAAGCCTTTCTCGGTTGACCGAGTTACACAGACCAAATCTCGCTCTCGATTTCGACCGAGATTTAGCCGGGAAATTTGGCAGAACTCGACCCTTTGGTTGACCGAGAATTATAGAAGTCAGAAGGTCTCCTTTGGTAAGAGAGATTTTGACCTCATAACCGGCCTTCGTCATTCATTTAGACCAATGAGGAGAGATAGAGAGGGCCCTCCCAATAGACTCCTAAGATTATATTTTAGAGAGAATGTAGGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACTCTTCAGTTTGAGAACGACGAAGATGCAGTTAAGATCGCAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCTAGCACCTTAGACTTAATGGATGATTGGGTGGCATTCTGCAACGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGTTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATATGGTTTCCCGTTTGCTTTTCAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGTAAGTGAGACAGCCATCCCACGCATCCGTCGATGGTCTTGCGCCCACTCCCCATCATACACGATCATTGCTGATGAAGTTTTTGGATCCCGGGCGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGACCATGAGAACGACACGTTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACAGCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTCGAAACAGGCGAATTTGTGTTGACAGACTCCCTAACGGCACTGAATTCAGATGCAGACATAGCCAAGCAGGTGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGACAAGCCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTCTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGCTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAG

Protein sequence

MGRGRPRPPRVFKNSEVFWDEPSGTGAIWGITGRKEVTELGLDHMGRAKSSYESDFWSYLCPIVLVSSLFTFLLSRLLHFFLTFVCCFLGNISRKSSLLLHELSSSAVSEEGRGKKNRILCCLRQRFWNRDESRPGFIFCNFSVNREIWQNSKPFSVDRVTQTKSRSRFRPRFSREIWQNSTLWLTENYRSQKVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLRLYFRENVGMKVEELDKSFPTLQFENDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRRWSCAHSPSYTIIADEVFGSRAVESCFFCRGESIHESSDHENDTFDWSRFKTVTNYVMGEHTDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQVNTVCTIFPRLLLRCDVMKDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFCRRQFAVQLWANRPFF

Homology

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BLAST of Lag0034564 vs. NCBI nr
Match: XP_022157020.1 (uncharacterized protein LOC111023847 [Momordica charantia])

HSP 1 Score: 218.8 bits (556), Expect = 1.2e-52
Identity = 113/216 (52.31%), Postives = 151/216 (69.91%), Query Frame = 0


Query: 175 REIWQNSTLWLTENYRSQKVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLR-LYFREN 234
           RE+ +     ++ N    +VSFGKR+FDLITGLRH+   M R  E   NR LR LYF++ 
Sbjct: 68  REVEEPKDDLISFNLFGNRVSFGKREFDLITGLRHT---MNRVDEDVRNRRLRILYFQDK 127

Query: 235 VGMKVEELDKSFPTLQFENDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFC 294
             +K  EL+K F    FENDEDAVKIA  YF ELA+MG+ERK ++D S L ++D W  FC
Sbjct: 128 ASVKCSELEKIFLEHTFENDEDAVKIAIVYFIELAMMGKERKLKMDTSLLGIVDRWEVFC 187

Query: 295 NEDWSTIVFDKTIKSLKKALKGKVESYKRK-GSGPKKQETYSLYGFPFAFQIWAYETVSS 354
           N DWS+++F++T+ SLK ALK KVE YK+K        ETYSLY FP+AFQ+WAYET+S+
Sbjct: 188 NYDWSSMIFERTLWSLKNALKDKVEXYKQKVAMDSSHVETYSLYXFPYAFQVWAYETIST 247

Query: 355 LTGRVANRVSETAIPRIRRWSCAHSPSYTIIADEVF 389
           L+ RVA R+++ AIPR+ RWSC +S ++ ++  EVF
Sbjct: 248 LSTRVALRLNDDAIPRLLRWSCTYSRAFNVLEREVF 280

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BLAST of Lag0034564 vs. NCBI nr
Match: KAA0038137.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 213.4 bits (542), Expect = 5.2e-51
Identity = 136/450 (30.22%), Postives = 220/450 (48.89%), Query Frame = 0


Query: 198 KRDFDLITGLRHSFRPMRRDREGPPN-RLLRLYFRENVGMKVEELDKSFPTLQFE-NDED 257
           +R+F++ITGL   + P     +   N RLL  +F++   + V +L+  F  L++E +D+D
Sbjct: 16  RREFNIITGL---WGPKEDYIQLVGNSRLLEKFFKDKECIYVSDLEDIF--LEYEDDDDD 75

Query: 258 AVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKG 317
            VK+A  YF E++L+G++R+ +VD     + DDW  F N DW  IVF++T+ +LK+AL  
Sbjct: 76  IVKLALVYFIEISLLGKDRRTKVDIGFFKIADDWNTFNNYDWGRIVFERTLSALKRALDK 135

Query: 318 KVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRRWSCA 377
           +    K+K +  KK   Y++ GFP A Q+WAYE++ ++ G   ++V++ AIPR+ RW C 
Sbjct: 136 QYAKGKKKSTQTKK---YTINGFPHALQVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQ 195

Query: 378 HSPSYTIIAD--------------------------------------EVF---GSRAVE 437
            SP    I+                                       E F    S+ ++
Sbjct: 196 QSPKSQTISQVFDSLMFIIKAVIEMTPEEKQLKIASAPLNKRTDDELRETFHGKKSKHLD 255

Query: 438 SCF-----------------FCRGESI-----------HESSDHENDTFDWSRFKTVTNY 497
           + F                 F   ++I           ++    EN  FDW     + +Y
Sbjct: 256 ALFLFIRLKIKAAGIPSSQNFTTADTIFMLILVSKWPLYKECIKENRPFDWDEEYRLVDY 315

Query: 498 VMGEHTDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQV 557
           V+G   D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   +
Sbjct: 316 VVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNIL 375

Query: 558 NTVCTIFPRLLLRCDVM--KDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVT 575
             +  + P+LL        + + S     W       +P Q+++ DCGVFT+K+ EY   
Sbjct: 376 LPIRQLVPKLLDSTGFFDRRGRSSTYKEHWPVVIVDSIPLQRNNSDCGVFTIKYFEYIAA 435

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BLAST of Lag0034564 vs. NCBI nr
Match: XP_022153201.1 (uncharacterized protein LOC111020757 [Momordica charantia])

HSP 1 Score: 198.7 bits (504), Expect = 1.3e-46
Identity = 101/198 (51.01%), Postives = 134/198 (67.68%), Query Frame = 0


Query: 192 QKVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLRLYFRENVGMKVEELDKSFPTLQFE 251
           ++VSFGKR+FDLITGL H  R  R D   P  RL   YF++ V +K  EL+K F    F 
Sbjct: 85  KRVSFGKREFDLITGLSH--RMNRVDNHIPGRRLRARYFKDGVRVKCSELEKIFLEDVFY 144

Query: 252 NDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKK 311
           +DED VK+   YF ELA+MG+ERKQ +D + L ++D W  FCN DWS+++FD+TI SLK 
Sbjct: 145 DDEDVVKVRIVYFIELAMMGKERKQFIDTALLGVVDRWEVFCNYDWSSMIFDRTIWSLKN 204

Query: 312 ALKGKVESYKRKGSG-PKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIR 371
           ALK K+  Y++K +  P   ETYSLYGFP+AFQ+WAYET+S+L        S+ AIPR+ 
Sbjct: 205 ALKDKLSVYQQKATADPSHVETYSLYGFPYAFQVWAYETISTL--------SDDAIPRLL 264

Query: 372 RWSCAHSPSYTIIADEVF 389
           RWSC +S  + ++  EVF
Sbjct: 265 RWSCIYSCGFRVLTSEVF 272

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BLAST of Lag0034564 vs. NCBI nr
Match: AAS91798.1 (Ulp1-like peptidase [Cucumis melo] >AAU04774.1 Ulp1 peptidase-like [Cucumis melo])

HSP 1 Score: 184.9 bits (468), Expect = 2.0e-42
Identity = 117/382 (30.63%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 195 SFGKRDFDLITGLRHSFRPMRRD--REGPPNRLLRLYFRENVGMKVEELDKSFPTLQFE- 254
           +FG+R+F+++TGL   + P + D  + G  +RLL  +F++   + V +L+  F  L++E 
Sbjct: 96  TFGRREFNIVTGL---WGP-KEDYIQLGGNSRLLEKFFKDKDCVYVSDLEDIF--LEYEG 155

Query: 255 NDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKK 314
           +D+D VK+A  YF E++L+G++R+ +VD   L + DDW +F N DW  IVF +T+ +LK+
Sbjct: 156 DDDDIVKLALVYFIEISLLGKDRRTKVDIGFLKIADDWNSFNNYDWGRIVFVRTLSALKR 215

Query: 315 ALKGKVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRR 374
           AL  +    K+K +  KK   Y++ GFP A Q            R+           + +
Sbjct: 216 ALDKQYAKGKKKSTQTKK---YTINGFPHALQ------------RIL----------VSK 275

Query: 375 WSCAHSPSYTIIADEVFGSRAVESCFFCRGESIHESSDHENDTFDWSRFKTVTNYVMGEH 434
           W                               +++    EN  FDW     + +YV+G  
Sbjct: 276 W------------------------------PLYKECIKENRPFDWDEEYRLVDYVVGSK 335

Query: 435 TDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQVNTVCT 494
            D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   +  +  
Sbjct: 336 VDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIRQ 395

Query: 495 IFPRLLLRCDVM--KDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLG 554
           + P+LL        + + S    PW       +P Q+++ DCGVF +K+ EY      L 
Sbjct: 396 LVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDPIPLQRNNCDCGVFAIKYFEYIAAGVGLD 416

Query: 555 SLSQEKIEFCRRQFAVQLWANR 572
           +L QE + + R+Q A Q+W N+
Sbjct: 456 TLCQENMSYFRKQLAFQVWTNQ 416

[Close]

BLAST of Lag0034564 vs. NCBI nr
Match: XP_038882332.1 (uncharacterized protein LOC120073583 [Benincasa hispida])

HSP 1 Score: 179.9 bits (455), Expect = 6.4e-41
Identity = 85/164 (51.83%), Postives = 112/164 (68.29%), Query Frame = 0


Query: 411 ENDTFDWSRFKTVTNYVMGEHTDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLT 470
           +  T DWS  K V  YV G+HTDY VPWS+VDAVYMPFNL   HWVL+CADF+  E ++ 
Sbjct: 56  DRHTIDWSMEKKVLKYVHGKHTDYDVPWSNVDAVYMPFNLSGMHWVLVCADFQVRELIMF 115

Query: 471 DSLTALNSDADIAKQVNTVCTIFPRLLLRCDVMKDKPSLPTHPWRFRRKTQVPQQQDSGD 530
           DSL AL+ +AD+  ++  VC  FP LL+   VM +  +L    W  RR     QQ +SGD
Sbjct: 116 DSLIALHLNADLECEMRLVCKNFPGLLIVGAVM-ESHNLLIDRWTLRRDAFRSQQHESGD 175

Query: 531 CGVFTVKFLEYDVTRSDLGSLSQEKIEFCRRQFAVQLWANRPFF 575
           CG+FT KF EYDVT S +G+L+Q++ ++ RRQ+A+Q+WANR  F
Sbjct: 176 CGMFTYKFFEYDVTGSKMGTLTQDRKQYFRRQYAIQIWANRALF 218

[Close]

BLAST of Lag0034564 vs. ExPASy TrEMBL
Match: A0A6J1DRZ7 (uncharacterized protein LOC111023847 OS=Momordica charantia OX=3673 GN=LOC111023847 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 6.0e-53
Identity = 113/216 (52.31%), Postives = 151/216 (69.91%), Query Frame = 0


Query: 175 REIWQNSTLWLTENYRSQKVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLR-LYFREN 234
           RE+ +     ++ N    +VSFGKR+FDLITGLRH+   M R  E   NR LR LYF++ 
Sbjct: 68  REVEEPKDDLISFNLFGNRVSFGKREFDLITGLRHT---MNRVDEDVRNRRLRILYFQDK 127

Query: 235 VGMKVEELDKSFPTLQFENDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFC 294
             +K  EL+K F    FENDEDAVKIA  YF ELA+MG+ERK ++D S L ++D W  FC
Sbjct: 128 ASVKCSELEKIFLEHTFENDEDAVKIAIVYFIELAMMGKERKLKMDTSLLGIVDRWEVFC 187

Query: 295 NEDWSTIVFDKTIKSLKKALKGKVESYKRK-GSGPKKQETYSLYGFPFAFQIWAYETVSS 354
           N DWS+++F++T+ SLK ALK KVE YK+K        ETYSLY FP+AFQ+WAYET+S+
Sbjct: 188 NYDWSSMIFERTLWSLKNALKDKVEXYKQKVAMDSSHVETYSLYXFPYAFQVWAYETIST 247

Query: 355 LTGRVANRVSETAIPRIRRWSCAHSPSYTIIADEVF 389
           L+ RVA R+++ AIPR+ RWSC +S ++ ++  EVF
Sbjct: 248 LSTRVALRLNDDAIPRLLRWSCTYSRAFNVLEREVF 280

[Close]

BLAST of Lag0034564 vs. ExPASy TrEMBL
Match: A0A5A7T951 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003630 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.5e-51
Identity = 136/450 (30.22%), Postives = 220/450 (48.89%), Query Frame = 0


Query: 198 KRDFDLITGLRHSFRPMRRDREGPPN-RLLRLYFRENVGMKVEELDKSFPTLQFE-NDED 257
           +R+F++ITGL   + P     +   N RLL  +F++   + V +L+  F  L++E +D+D
Sbjct: 16  RREFNIITGL---WGPKEDYIQLVGNSRLLEKFFKDKECIYVSDLEDIF--LEYEDDDDD 75

Query: 258 AVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKG 317
            VK+A  YF E++L+G++R+ +VD     + DDW  F N DW  IVF++T+ +LK+AL  
Sbjct: 76  IVKLALVYFIEISLLGKDRRTKVDIGFFKIADDWNTFNNYDWGRIVFERTLSALKRALDK 135

Query: 318 KVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRRWSCA 377
           +    K+K +  KK   Y++ GFP A Q+WAYE++ ++ G   ++V++ AIPR+ RW C 
Sbjct: 136 QYAKGKKKSTQTKK---YTINGFPHALQVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQ 195

Query: 378 HSPSYTIIAD--------------------------------------EVF---GSRAVE 437
            SP    I+                                       E F    S+ ++
Sbjct: 196 QSPKSQTISQVFDSLMFIIKAVIEMTPEEKQLKIASAPLNKRTDDELRETFHGKKSKHLD 255

Query: 438 SCF-----------------FCRGESI-----------HESSDHENDTFDWSRFKTVTNY 497
           + F                 F   ++I           ++    EN  FDW     + +Y
Sbjct: 256 ALFLFIRLKIKAAGIPSSQNFTTADTIFMLILVSKWPLYKECIKENRPFDWDEEYRLVDY 315

Query: 498 VMGEHTDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQV 557
           V+G   D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   +
Sbjct: 316 VVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNIL 375

Query: 558 NTVCTIFPRLLLRCDVM--KDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVT 575
             +  + P+LL        + + S     W       +P Q+++ DCGVFT+K+ EY   
Sbjct: 376 LPIRQLVPKLLDSTGFFDRRGRSSTYKEHWPVVIVDSIPLQRNNSDCGVFTIKYFEYIAA 435

[Close]

BLAST of Lag0034564 vs. ExPASy TrEMBL
Match: A0A6J1DJX9 (uncharacterized protein LOC111020757 OS=Momordica charantia OX=3673 GN=LOC111020757 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 6.4e-47
Identity = 101/198 (51.01%), Postives = 134/198 (67.68%), Query Frame = 0


Query: 192 QKVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLRLYFRENVGMKVEELDKSFPTLQFE 251
           ++VSFGKR+FDLITGL H  R  R D   P  RL   YF++ V +K  EL+K F    F 
Sbjct: 85  KRVSFGKREFDLITGLSH--RMNRVDNHIPGRRLRARYFKDGVRVKCSELEKIFLEDVFY 144

Query: 252 NDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKK 311
           +DED VK+   YF ELA+MG+ERKQ +D + L ++D W  FCN DWS+++FD+TI SLK 
Sbjct: 145 DDEDVVKVRIVYFIELAMMGKERKQFIDTALLGVVDRWEVFCNYDWSSMIFDRTIWSLKN 204

Query: 312 ALKGKVESYKRKGSG-PKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIR 371
           ALK K+  Y++K +  P   ETYSLYGFP+AFQ+WAYET+S+L        S+ AIPR+ 
Sbjct: 205 ALKDKLSVYQQKATADPSHVETYSLYGFPYAFQVWAYETISTL--------SDDAIPRLL 264

Query: 372 RWSCAHSPSYTIIADEVF 389
           RWSC +S  + ++  EVF
Sbjct: 265 RWSCIYSCGFRVLTSEVF 272

[Close]

BLAST of Lag0034564 vs. ExPASy TrEMBL
Match: Q5GIS9 (Ulp1 peptidase-like OS=Cucumis melo OX=3656 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 9.6e-43
Identity = 117/382 (30.63%), Postives = 193/382 (50.52%), Query Frame = 0


Query: 195 SFGKRDFDLITGLRHSFRPMRRD--REGPPNRLLRLYFRENVGMKVEELDKSFPTLQFE- 254
           +FG+R+F+++TGL   + P + D  + G  +RLL  +F++   + V +L+  F  L++E 
Sbjct: 96  TFGRREFNIVTGL---WGP-KEDYIQLGGNSRLLEKFFKDKDCVYVSDLEDIF--LEYEG 155

Query: 255 NDEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKK 314
           +D+D VK+A  YF E++L+G++R+ +VD   L + DDW +F N DW  IVF +T+ +LK+
Sbjct: 156 DDDDIVKLALVYFIEISLLGKDRRTKVDIGFLKIADDWNSFNNYDWGRIVFVRTLSALKR 215

Query: 315 ALKGKVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRR 374
           AL  +    K+K +  KK   Y++ GFP A Q            R+           + +
Sbjct: 216 ALDKQYAKGKKKSTQTKK---YTINGFPHALQ------------RIL----------VSK 275

Query: 375 WSCAHSPSYTIIADEVFGSRAVESCFFCRGESIHESSDHENDTFDWSRFKTVTNYVMGEH 434
           W                               +++    EN  FDW     + +YV+G  
Sbjct: 276 W------------------------------PLYKECIKENRPFDWDEEYRLVDYVVGSK 335

Query: 435 TDYSVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQVNTVCT 494
            D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   +  +  
Sbjct: 336 VDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIRQ 395

Query: 495 IFPRLLLRCDVM--KDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLG 554
           + P+LL        + + S    PW       +P Q+++ DCGVF +K+ EY      L 
Sbjct: 396 LVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDPIPLQRNNCDCGVFAIKYFEYIAAGVGLD 416

Query: 555 SLSQEKIEFCRRQFAVQLWANR 572
           +L QE + + R+Q A Q+W N+
Sbjct: 456 TLCQENMSYFRKQLAFQVWTNQ 416

[Close]

BLAST of Lag0034564 vs. ExPASy TrEMBL
Match: A0A6J1E0A9 (uncharacterized protein LOC111025209 OS=Momordica charantia OX=3673 GN=LOC111025209 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.0e-40
Identity = 92/201 (45.77%), Postives = 132/201 (65.67%), Query Frame = 0


Query: 193 KVSFGKRDFDLITGLRHSFRPMRRDREGPPNRLLRLYFRENVGMKVEELDKSFPTLQFEN 252
           KVSFG+R+FD+I+GL++S  P+R+     P R   LYF  +  + + EL+K + +++FE+
Sbjct: 63  KVSFGRREFDIISGLKYSRSPVRKITY--PQRHGTLYFNNSTDLLLSELEKMYTSIRFED 122

Query: 253 DEDAVKIAAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKKA 312
           D DAVK+   YF EL L+GRER  + D   L ++DDW A CN DW+ + FDKTI SL++ 
Sbjct: 123 DIDAVKVLV-YFVELVLLGRERSTKFDHILLGIVDDWEACCNHDWALLSFDKTIYSLQRG 182

Query: 313 LKGKVESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRVSETAIPRIRRW 372
                 S K K  G +K  +YSLYGFP+AFQ+WAYE +SSL+G +   VS+  +PRI +W
Sbjct: 183 -----ASNKSKEGGLRK--SYSLYGFPWAFQVWAYEIISSLSGHIVTIVSQDVVPRILQW 242

Query: 373 SCAHSPSYTIIADEVFGSRAV 394
              HS +Y ++A E+F S  V
Sbjct: 243 RYEHSTAYHMLAREIFRSSTV 253

[Close]

BLAST of Lag0034564 vs. TAIR 10
Match: AT5G45570.1 (Ulp1 protease family protein )

HSP 1 Score: 63.2 bits (152), Expect = 8.1e-10
Identity = 36/129 (27.91%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 441 VDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQVNTVCTIFPRLLLRC 500
           VD +Y    +  NHWV L  D       + DS+ +L +D ++A Q   V T+ P +L   
Sbjct: 768 VDHLYAYLFVNGNHWVALDIDLTNKRVNVYDSIPSLTTDTEMAIQCMFVMTMIPAMLSSF 827

Query: 501 DVMKD-KPSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFC 560
              K  + S     W  +R T++P+  D GDC ++++K++E          L  E ++  
Sbjct: 828 IPSKQRRRSYSKLEW--KRITKIPENLDPGDCAIYSIKYIECLALGKSFDGLCDENMQSL 887

Query: 561 RRQFAVQLW 569
           R + AV+++
Sbjct: 888 RTKLAVEMF 894

[Close]

BLAST of Lag0034564 vs. TAIR 10
Match: AT2G07240.1 (cysteine-type peptidases;cysteine-type peptidases )

HSP 1 Score: 56.6 bits (135), Expect = 7.6e-08
Identity = 30/99 (30.30%), Postives = 45/99 (45.45%), Query Frame = 0


Query: 438 WSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTALNSDADIAKQVNTVCTIFPRLL 497
           ++  D VYMPFN  + HWV LC D +  +  + DS   L  DA +  ++  +  + P L 
Sbjct: 738 FAEADCVYMPFNFDKKHWVSLCVDLKAHKITILDSNIQLRRDAALYAELQPLAAMLPYLF 797

Query: 498 LRCDVMKDKPSLPTHPWRFRRKTQVPQQQDSGDCGVFTV 537
            +        SL   P+   R   +PQ     D GV +V
Sbjct: 798 RQTSSSSGDISL--QPFLLDRPHGIPQVSSPFDSGVLSV 834

The following BLAST results are available for this feature:

BLAST of Lag0034564 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157020.1	1.2e-52	52.31	uncharacterized protein LOC111023847 [Momordica charantia]	[more]
KAA0038137.1	5.2e-51	30.22	Ulp1-like peptidase [Cucumis melo var. makuwa]	[more]
XP_022153201.1	1.3e-46	51.01	uncharacterized protein LOC111020757 [Momordica charantia]	[more]
AAS91798.1	2.0e-42	30.63	Ulp1-like peptidase [Cucumis melo] >AAU04774.1 Ulp1 peptidase-like [Cucumis melo...	[more]
XP_038882332.1	6.4e-41	51.83	uncharacterized protein LOC120073583 [Benincasa hispida]	[more]

BLAST of Lag0034564 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0034564 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DRZ7	6.0e-53	52.31	uncharacterized protein LOC111023847 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A5A7T951	2.5e-51	30.22	Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G0...	[more]
A0A6J1DJX9	6.4e-47	51.01	uncharacterized protein LOC111020757 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
Q5GIS9	9.6e-43	30.63	Ulp1 peptidase-like OS=Cucumis melo OX=3656 PE=3 SV=1	[more]
A0A6J1E0A9	2.0e-40	45.77	uncharacterized protein LOC111025209 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]

BLAST of Lag0034564 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45570.1	8.1e-10	27.91	Ulp1 protease family protein	[more]
AT2G07240.1	7.6e-08	30.30	cysteine-type peptidases;cysteine-type peptidases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 379..571 e-value: 7.2E-25 score: 89.7
None	No IPR available	PANTHER	PTHR12606	SENTRIN/SUMO-SPECIFIC PROTEASE	coord: 422..570
None	No IPR available	PANTHER	PTHR12606:SF109	BNACNNG52470D PROTEIN	coord: 422..570
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 437..566 e-value: 2.6E-20 score: 73.2
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 299..542 score: 13.742077
IPR015410	Domain of unknown function DUF1985	PFAM	PF09331	DUF1985	coord: 184..308 e-value: 2.3E-11 score: 43.8
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 414..569

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034564.1	Lag0034564.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity