Lag0034450 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034450 vs. NCBI nr
Match: KAF2303938.1 (hypothetical protein GH714_024716 [Hevea brasiliensis])

HSP 1 Score: 1860.5 bits (4818), Expect = 0.0e+00
Identity = 961/1559 (61.64%), Postives = 1204/1559 (77.23%), Query Frame = 0

Query: 1    MDDSGNNSSEIGERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCP 60
            M D+GN     G   HTPPKDFVCPIT ++F DPVTLETGQTYER AIQEWLDRGNSTCP
Sbjct: 383  MGDTGNPP---GAGKHTPPKDFVCPITSHLFDDPVTLETGQTYERKAIQEWLDRGNSTCP 442

Query: 61   ITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVSDKALD---------EANPVAVLTSPV 120
            IT QKL +TQLPKTNYVLKRL+ASW E+NP+F+S+++           ++  +  +TSP 
Sbjct: 443  ITRQKLHSTQLPKTNYVLKRLVASWQEQNPNFISNQSETPHQKTEPSFKSTIMPPITSPN 502

Query: 121  SVISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPV 180
            SVISQA+I+ + +E+RHAITNL  SE+L E+E AVL +E+FW E N++ D+Q ML KPPV
Sbjct: 503  SVISQATIDSTMSELRHAITNLCMSEILNESEMAVLRIEQFWQEANMDPDVQSMLSKPPV 562

Query: 181  INGLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAV 240
            ING VEIL NS++ QVL+AT+FLLSELG +D  VIQTLTRVESDV+CIV LFK GL+EAV
Sbjct: 563  INGFVEILFNSIDPQVLKATIFLLSELGSRDKGVIQTLTRVESDVECIVALFKKGLLEAV 622

Query: 241  VLIYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSL 300
            VLIY L  S  SL EMDM  SLL V+KKKE DV KMR+  K+A+V+LL +IL  S+E ++
Sbjct: 623  VLIYLLRPSTMSLLEMDMVESLLTVIKKKE-DVIKMRLKPKTASVLLLGQILCGSEESTV 682

Query: 301  IAV--AVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILE 360
             ++  A+++    ESI  SL+A   EERIAAVGILLRC+QEDGKCRN IADKA+L  +LE
Sbjct: 683  FSIVNAIVSTKVIESISGSLEADWAEERIAAVGILLRCMQEDGKCRNTIADKAELGPVLE 742

Query: 361  SFMEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQS 420
            +FM  S+ E+FEI+ F SELVKLNRRTFNEQ+L  IK+ G  STMHSLL YLQTAL+DQ 
Sbjct: 743  TFMSASDGERFEIVHFFSELVKLNRRTFNEQVLHIIKDEGAFSTMHSLLNYLQTALQDQC 802

Query: 421  PVVAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTS 480
            PVVAGLLLQLD+LVEPRKMSIYREEAID LISCLG  +FP AQI+AA TIMSLQGRF+ S
Sbjct: 803  PVVAGLLLQLDLLVEPRKMSIYREEAIDNLISCLGKYEFPVAQIAAAHTIMSLQGRFTAS 862

Query: 481  GRPLARYVLLERVGFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHD 540
            G+ L R  LL+R G  K +  L R D   +  GE+E T+EEERAA+EWERKMAF LVSH+
Sbjct: 863  GKSLGRAFLLKRAGHSKSYRYLMRMDQLGNLSGEIEETLEEERAAEEWERKMAFALVSHE 922

Query: 541  FGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFAT 600
            FGL+FE LA+GLKS+ A L SACFVSATWL +ML VLPDTGI  AARVCLL  F++IF +
Sbjct: 923  FGLVFEALAEGLKSRNAELCSACFVSATWLVYMLGVLPDTGIRGAARVCLLKRFVTIFKS 982

Query: 601  TTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGED 660
              D E++ L L+A+ SFI++PEG + L+S +K+I +GL+EL++S+PLA E+LKVL EG +
Sbjct: 983  AKDTEDRVLSLLALKSFINDPEGLRDLTSYVKDIKKGLRELKKSSPLALEVLKVLSEGHE 1042

Query: 661  SSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEH 720
            SS E W+H+ L QVDCS+NGEVLSI  FKDKI SGHSDGTIKVW+ R +I  L+QEI+EH
Sbjct: 1043 SSAEFWNHEELTQVDCSDNGEVLSITCFKDKIFSGHSDGTIKVWTGRGSILQLIQEIREH 1102

Query: 721  SKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFI 780
            +KAVTSL ++ S   LYSGSLD+T +VWS+G++ + C++VHD+KDQ+HNLVV+ +++CFI
Sbjct: 1103 TKAVTSLVVLHSGDSLYSGSLDRTARVWSIGNEALHCVKVHDMKDQVHNLVVANSISCFI 1162

Query: 781  PHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGS 840
            P GAG++V+SW G  K LNS+K+VKCL+LV GKLYCGC DSSIQ +DLAT T   I +GS
Sbjct: 1163 PQGAGVKVHSWNGGFKFLNSNKYVKCLSLVQGKLYCGCHDSSIQEIDLATVTSVTIQNGS 1222

Query: 841  RKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSD 900
            RKLLGKANP+ AL   D  ++SAS++LDG AVKIWS S Y  +GS+ T ++VR M +SS+
Sbjct: 1223 RKLLGKANPIHALHVRDGLIYSASSALDGAAVKIWSASTYGLVGSVPTTLEVRAMAISSE 1282

Query: 901  LIYLGGR-GVVEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPA 960
            LIYLG + G VEIW + K  +I+TLQTG + +V C+ L+  EEVLVIGTSDG IQ     
Sbjct: 1283 LIYLGSKGGTVEIWDQKKQNRIETLQTGTDGRVLCIALDGNEEVLVIGTSDGRIQ----- 1342

Query: 961  MNMCPEPSHYTMEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGL 1020
                             + METTS++  T Q V  KKGGLRTMPFIIANE FEK++ VGL
Sbjct: 1343 ---------------EKQGMETTSDERNTIQNVKRKKGGLRTMPFIIANETFEKVAGVGL 1402

Query: 1021 HANMIFYLTNEYNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLL 1080
            HANMI YL NEYN+ +A GA +LF+W+A + F PI GAF+SDSYLGRF+VI +GTVV+LL
Sbjct: 1403 HANMILYLQNEYNLSSATGANILFIWAATSYFTPIIGAFVSDSYLGRFRVIVLGTVVSLL 1462

Query: 1081 GMVVLWLTAILPKARPPHCKSPG---EFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLA 1140
            GM++LWLTAI+P ARP HC       + C S +A Q+M L  SF LMA+GAGGIRPCSLA
Sbjct: 1463 GMILLWLTAIIPSARPLHCDQKDGNLQGCASPNAAQMMFLLSSFALMAVGAGGIRPCSLA 1522

Query: 1141 FGADQLEKPGDHKNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLM 1200
            FGADQL+ P + KN +T+QSFFNWYY SVG+SV +SVIF+V +Q+AAGWVVG+ VPVG M
Sbjct: 1523 FGADQLDNPENPKNDKTLQSFFNWYYASVGISVMVSVIFVVAIQDAAGWVVGFAVPVGFM 1582

Query: 1201 LFSTIMFFIGSPLYVKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVT 1260
            L S ++F +GS  YVK+  N SL +  AQVI AAWKN+++ LP   SD+W++HK S LV 
Sbjct: 1583 LLSIVLFLMGSSRYVKVKPNTSLLTGFAQVISAAWKNKYISLPRSDSDRWYHHKDSTLVI 1642

Query: 1261 PTPKLRFLNKACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAA 1320
            PT KL FLNKAC+I N E D+D  G+A   W+L T+K+VEELKA+I+V+PIWSTGI+++A
Sbjct: 1643 PTEKLSFLNKACVIINPEKDLDKEGLAIDAWKLCTVKQVEELKALIKVMPIWSTGIMLSA 1702

Query: 1321 TIHQFTFAALQAITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRS 1380
            T+ Q  F  LQA+TMDRH+    + PAASF VFTILTLTIWVAIYD I++P +AKFT+R 
Sbjct: 1703 TLSQHAFPVLQALTMDRHLVGKVKIPAASFGVFTILTLTIWVAIYDRILVPRIAKFTKRP 1762

Query: 1381 NGFTFKQRMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCL 1440
             G + KQRMGIGL +SC+++A++  +E KRR  AI EG+A+ P G+V MSAMWLVPQ+C+
Sbjct: 1763 RGLSNKQRMGIGLLLSCISTAIAGAVEHKRRAMAIREGLAHKPMGLVNMSAMWLVPQHCV 1822

Query: 1441 AGLAEAFNAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNL 1500
             GLAEAFNAIGQI+F+YSQ P++M+SI VALFSLGM  G+LL + IVS++K+ET KNG +
Sbjct: 1823 TGLAEAFNAIGQIEFYYSQFPKTMSSIGVALFSLGMAFGNLLGSFIVSILKRETGKNGKV 1882

Query: 1501 GWLPNNLNKGHYDYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEEE 1545
             W+ NNLN+GHYDYYYW+L+ + V+NF YYL+CS FYG E      +R+WD   AIEE+
Sbjct: 1883 SWVANNLNRGHYDYYYWLLASLSVLNFFYYLLCSRFYGSEN-----NRIWD--AAIEEK 1910

BLAST of Lag0034450 vs. NCBI nr
Match: THG10992.1 (hypothetical protein TEA_024355 [Camellia sinensis var. sinensis])

HSP 1 Score: 1827.4 bits (4732), Expect = 0.0e+00
Identity = 937/1539 (60.88%), Postives = 1185/1539 (77.00%), Query Frame = 0

Query: 12   GERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQL 71
            G   HTPPKDFVCPIT NIF DPVTLETGQTYER AIQEWL+RGN TCPIT QKL  TQL
Sbjct: 413  GGEKHTPPKDFVCPITSNIFGDPVTLETGQTYERKAIQEWLERGNCTCPITRQKLHRTQL 472

Query: 72   PKTNYVLKRLIASWLEENPSFV---SDKALDEANPV----AVLTSPVSVISQASINRSTN 131
            PKTNYVLKRLIASW E+NP+ +   S+ +  E+ PV       TSP SVISQA+I+ + +
Sbjct: 473  PKTNYVLKRLIASWQEQNPTSIPVQSENSQPESKPVFNSMMRSTSPNSVISQATIDGTVS 532

Query: 132  EVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEILVNSVN 191
            E+   ITNL  SE+L+E+E AVL +ERFW E N+E++IQ ML KPPVING VEIL NSV+
Sbjct: 533  ELHLVITNLCMSEILKESEMAVLQIERFWQEANMEVEIQAMLSKPPVINGFVEILFNSVD 592

Query: 192  LQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGLSIESL 251
             QVL AT+FLLSELG +D  VIQTLTRV+SDV+C+V LF +GL+E VVLIY L  S  SL
Sbjct: 593  PQVLTATIFLLSELGSRDGAVIQTLTRVDSDVECVVALFTNGLLETVVLIYLLRPSSISL 652

Query: 252  QEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSL--IAVAVLAENAT 311
             +MDM  +LL V++ KE D+ KM M  K+AAV+LL +I G ++E SL  I   V+     
Sbjct: 653  IKMDMVDTLLTVIRNKEDDMIKMCMKPKTAAVLLLGQIFGSAEEISLSEITRRVIMGKVI 712

Query: 312  ESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQFEI 371
            ESI+ SL++   EERIAAVGILLRC+Q +GKCRN IADKA+L  +LE F   S+ E+FEI
Sbjct: 713  ESIVASLQSDWAEERIAAVGILLRCMQGEGKCRNSIADKAELAPVLECFTGASDGERFEI 772

Query: 372  ILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQLDIL 431
            + FL ELVKLNRRTFNEQIL  IK+ G  STMH+LLIYLQTAL+DQ P+VAGLLLQLD+L
Sbjct: 773  VHFLFELVKLNRRTFNEQILHIIKDEGTLSTMHTLLIYLQTALQDQCPIVAGLLLQLDLL 832

Query: 432  VEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 491
             EPRKMSIYREEAID LISCL +SDFP AQI+AAETI++LQGRFS+SG+PLAR  LL+  
Sbjct: 833  EEPRKMSIYREEAIDTLISCLRNSDFPAAQIAAAETILALQGRFSSSGKPLARAFLLKHG 892

Query: 492  GFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAKGLK 551
            G  K H  + R++  ++  GE++ ++EEE+AA+EWERKMAFVLVSH+FGL+FE L +GLK
Sbjct: 893  GVDKSHKYVMRKEQLSTMSGEIQESLEEEKAAEEWERKMAFVLVSHEFGLVFEALGEGLK 952

Query: 552  SKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALGLIA 611
            S+YA L SACFVSA WL HML VLPDTG+  AARVCLL  F+S   +  D E+K L ++A
Sbjct: 953  SRYAELSSACFVSAAWLVHMLNVLPDTGLRGAARVCLLKCFVSTLKSARDTEDKVLSMLA 1012

Query: 612  MNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSST-ELWSHQVLF 671
            +NSF+H+PEG + ++ +MK+I++GL+EL++S+ +A +MLKV  E  DSST +LW+H+ L 
Sbjct: 1013 LNSFVHDPEGLRDITYHMKDILKGLRELKKSSTMALDMLKVFAEERDSSTQDLWNHKDLV 1072

Query: 672  QVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIMES 731
            Q DCS NGEVLS+ YF+DKI SGHSDGTIKVW+ + +I HL+QE ++H+KAV+SLAI++S
Sbjct: 1073 QEDCSVNGEVLSVVYFRDKIFSGHSDGTIKVWTGKGSILHLIQETRKHTKAVSSLAILQS 1132

Query: 732  EQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYSWG 791
              KLYSGSLDKT++VWS+G +++ C QVHD+KDQ+HNLVV+ +VACFIP GAGI+++SWG
Sbjct: 1133 GDKLYSGSLDKTVRVWSIGGEVMHCTQVHDMKDQVHNLVVANSVACFIPQGAGIKMHSWG 1192

Query: 792  GESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPVQA 851
            G SKLLN +K  KC+ L+HGKLY GCQD+SIQ +DLATGT++ I SGSRKLLGKANP+ A
Sbjct: 1193 GGSKLLNPNKSAKCITLLHGKLYSGCQDNSIQEIDLATGTLNTIQSGSRKLLGKANPIHA 1252

Query: 852  LQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GVVE 911
            L   D  L+SAS+SL+G AVKIW+ SNYS +GSL   ++VR M VSS+LIYLG + G VE
Sbjct: 1253 LNIRDGLLYSASSSLNGAAVKIWNASNYSMVGSLPLTLEVRAMVVSSELIYLGCKGGTVE 1312

Query: 912  IWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPAMNMCPEPSHYTM 971
            +W R KH ++D LQTG N +V CM L+  EEVLVIGTSDG                   M
Sbjct: 1313 VWCRKKHNRVDMLQTGSNGRVLCMALDADEEVLVIGTSDG------------------RM 1372

Query: 972  EKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEY 1031
            E+  ++ ++TTS      Q V+ +KGGLRTMPF+IANE  EK+++VGLHANMI YL NEY
Sbjct: 1373 EERSADPVKTTS------QPVSRRKGGLRTMPFVIANEALEKVASVGLHANMILYLKNEY 1432

Query: 1032 NVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILP 1091
            ++    G  +LF+W+A++NFLP  GAFLSDSYLGRF V+ +G++VTLLG+VVLW TAI  
Sbjct: 1433 HLSIVTGTSILFLWNAVSNFLPTFGAFLSDSYLGRFCVVGLGSIVTLLGLVVLWFTAIFK 1492

Query: 1092 KARPPHCKSPG-EFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKN 1151
             ARPPHC  P  + C S    QL LL+ SF+LMAIGAGGIRPCSLAFGADQ  KP + KN
Sbjct: 1493 HARPPHCDHPNTDHCASPKPAQLALLFSSFVLMAIGAGGIRPCSLAFGADQFNKPDNPKN 1552

Query: 1152 QRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLY 1211
             R +Q+FFNWYYVS+G+S+ ISV  +VY+Q A GW VG+GVPV LM FST+MFF+GSPL+
Sbjct: 1553 TRVLQTFFNWYYVSLGISIMISVTVIVYIQTAKGWTVGFGVPVVLMFFSTVMFFLGSPLF 1612

Query: 1212 VKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYH-KGSKLVTPTPKLRFLNKACI 1271
            VK+ ANKSL +  AQV+  +WKN+HL+ PP+ S+  ++H KGSKL+ P  KLRFLNKACI
Sbjct: 1613 VKVKANKSLVTGFAQVMAVSWKNKHLDFPPKDSEGQYHHKKGSKLLAPAEKLRFLNKACI 1672

Query: 1272 IRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQAI 1331
            I+N E D++ +G A  PW L T+++VEELKA+I+VLPIWS GI+IA TI Q  F  LQA 
Sbjct: 1673 IKNPEKDLNPDGTALDPWTLCTVQQVEELKALIKVLPIWSAGIIIAVTISQQAFPVLQAY 1732

Query: 1332 TMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGL 1391
            TMDRH   +F+ P  S+ VF+ILTLTIWVAIYD +I+P L+K+T+R  G + KQRMGIGL
Sbjct: 1733 TMDRHFNKNFKMPPGSYGVFSILTLTIWVAIYDRLIVPWLSKYTKRPRGLSLKQRMGIGL 1792

Query: 1392 AISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQI 1451
             +SC+A+ V+A IE  RR RAI EG+A+ P  +V MSAMWLVPQ+CL G++EAFNAIGQI
Sbjct: 1793 FLSCIATTVAALIESTRRARAINEGLADQPDAVVNMSAMWLVPQHCLVGISEAFNAIGQI 1852

Query: 1452 QFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHYD 1511
            QF+YSQ P+SM+SI +ALF+LGMG G+LL ++IVS++   T + GN+ W+ NNLN+GHYD
Sbjct: 1853 QFYYSQFPKSMSSIGIALFTLGMGVGNLLGSLIVSILAHATKRGGNVSWVSNNLNRGHYD 1912

Query: 1512 YYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDE 1538
            YYYW+L+ + V NF Y+L+C W YG E++      VWD+
Sbjct: 1913 YYYWILTCLSVANFFYFLLCGWAYGSEEQ-----NVWDD 1922

BLAST of Lag0034450 vs. NCBI nr
Match: KZM83883.1 (hypothetical protein DCAR_028695 [Daucus carota subsp. sativus])

HSP 1 Score: 1666.4 bits (4314), Expect = 0.0e+00
Identity = 883/1550 (56.97%), Postives = 1129/1550 (72.84%), Query Frame = 0

Query: 12   GERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQL 71
            G   H  P+DFVCPIT  IF DPVTLETGQTYER AIQEW+DRGN TCPIT QKL  TQL
Sbjct: 328  GGGKHKAPRDFVCPITSYIFDDPVTLETGQTYERKAIQEWIDRGNLTCPITRQKLNGTQL 387

Query: 72   PKTNYVLKRLIASWLEENPS---FVSDKALDEANPV----AVLTSPVSVISQASINRSTN 131
            P TNYVLKRLIA W E+N S     SD    E+ P     A L SP SVISQA+ + +  
Sbjct: 388  PNTNYVLKRLIARWREQNSSPTPVKSDNQHQESEPSFSNGAPLASPDSVISQATFDGAVG 447

Query: 132  EVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEILVNSVN 191
            E+RHAI  L  SE+L+E+E AVL +E+FW E N++ DIQ ML +P +ING V+IL NSV+
Sbjct: 448  ELRHAIETLSMSEILKESEMAVLWIEKFWKEANMQADIQTMLSEPSIINGFVDILFNSVD 507

Query: 192  LQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGLSIESL 251
             QVLRA+VFLLSELG +D  VIQTL  V+ DV+C+V LF+ GL+EAVVL+Y L  S  S 
Sbjct: 508  AQVLRASVFLLSELGSRDDSVIQTLCGVDPDVECVVALFRKGLLEAVVLVYLLRSSATSF 567

Query: 252  QEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSLIAV--AVLAENAT 311
             ++ +  SLLNV+ KKE D  KM +  +SA+V+LL++ILG + E S+     + +++   
Sbjct: 568  IDVGILDSLLNVLGKKEDDFVKMCIRPQSASVLLLKQILGSADEISISGALSSFISDEVI 627

Query: 312  ESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQFEI 371
            E+IL SLKA+   ER AAV ILLRCIQEDG  RN+IA KA+L  ++ESF+E + +E+F+I
Sbjct: 628  ENILYSLKAEWHGERCAAVSILLRCIQEDGNRRNLIAQKAELAPLIESFLEANEKERFQI 687

Query: 372  ILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQLDIL 431
            + FLSELVKL+RR  NE IL  IK+ G  STMH+LLIYLQTA +DQ PVVAGLLLQLDIL
Sbjct: 688  VQFLSELVKLHRRIDNENILHVIKDEGTFSTMHTLLIYLQTARQDQRPVVAGLLLQLDIL 747

Query: 432  VEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 491
            ++PRK SIYREEA+D +ISCL +SDFP AQI+AAETI+ LQGRFS SG+ LAR +LL R 
Sbjct: 748  MKPRKASIYREEAVDTIISCLRNSDFPDAQIAAAETILVLQGRFSYSGKSLARDLLLRRA 807

Query: 492  GFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAKGLK 551
            G  K +     +D            +EEE AA+EWE+K+AF LVSH+FGLLFE LA+GLK
Sbjct: 808  GLHKNYKSQMLKDQDMMTSQNSGEMMEEENAAEEWEKKVAFALVSHEFGLLFEALAEGLK 867

Query: 552  SKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALGLIA 611
            S  A L SACFVSATWL  ML  LPDTGI  AAR CLL+ F+SIF +  DIE++A+ ++A
Sbjct: 868  STNAKLHSACFVSATWLVDMLARLPDTGIRGAARACLLEQFVSIFKSAKDIEDRAVSMLA 927

Query: 612  MNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSSTELWSHQVLFQ 671
            ++SFIHEPEG Q L+  MK+I++GL+EL++ + +AFEMLK L +G+DSS E+W+H+ L Q
Sbjct: 928  LSSFIHEPEGLQDLTLYMKDILKGLRELKKFSVVAFEMLKSLADGDDSSNEMWNHKELVQ 987

Query: 672  VDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIMESE 731
             DCS NGEVLS+  FKDKI SGHSDGTIK W+ + +I HL+QE +EH+KAVTSL + ES 
Sbjct: 988  EDCSANGEVLSVVCFKDKIFSGHSDGTIKAWTGKGSILHLIQETREHTKAVTSLTVSESG 1047

Query: 732  QKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYSWGG 791
             KLYSGSLDKT++ W++ S+ +QC  V ++KD ++NL ++  ++C+IP+GAGI+V+S  G
Sbjct: 1048 DKLYSGSLDKTVRAWNVDSEGMQCEHVSEMKDHVNNLAIANNISCYIPNGAGIKVHSLNG 1107

Query: 792  ESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPVQAL 851
             SKLLN  K+VKCL LV GKL+CGC D+SIQ +DL TG +  I SGSR  + KANP+ AL
Sbjct: 1108 TSKLLNPKKYVKCLVLVQGKLFCGCHDNSIQEIDLETGALCTIQSGSRHFITKANPIYAL 1167

Query: 852  QAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GVVEI 911
            Q +D  ++SAS+ LDG AVKIWS S Y  IGS+ + ++VR+M V+SDLIYLG + G+VE+
Sbjct: 1168 QVHDGLIYSASSPLDGAAVKIWSASTYDIIGSVPSTLEVRSMEVTSDLIYLGCKGGIVEV 1227

Query: 912  WGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPAMNMCPEPSHYTME 971
            W R K  K +TLQTG N KV CM L+  EEVLVIGTS+G IQ+                 
Sbjct: 1228 WCRKKLDKKETLQTGTNGKVLCMALDSDEEVLVIGTSNGRIQM----------------- 1287

Query: 972  KYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYN 1031
            K+ SE              V  K GGLRTMPFIIANE FEK+++ GLHANMI YL  EY+
Sbjct: 1288 KFSSE-------------IVTRKLGGLRTMPFIIANEAFEKVASTGLHANMILYLVFEYH 1347

Query: 1032 VDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPK 1091
            +D   G  +LF+W+A+ NF+PI GAFLSDSYLGRF+VI+ GTVVTL+G ++LW TAI+P+
Sbjct: 1348 MDVVTGTTILFLWNAIANFMPILGAFLSDSYLGRFRVIAWGTVVTLIGTIILWFTAIIPE 1407

Query: 1092 ARPPHC--KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKN 1151
              PP C  + P + CV  D+GQL LL+ S  +MAIGAGGIRPCSLAFGADQ +KP + +N
Sbjct: 1408 FTPPDCDIRQPRD-CVKPDSGQLALLFISLAVMAIGAGGIRPCSLAFGADQFDKPDNPEN 1467

Query: 1152 QRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLY 1211
            +R +Q FFN YY SVG+S+ ISV  +VY+QN  GW+VG+GVPVG +L ST++F IGSPL+
Sbjct: 1468 ERILQRFFNLYYASVGISLMISVTVIVYIQNKFGWIVGFGVPVGCLLLSTVLFLIGSPLF 1527

Query: 1212 VKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYH-KGSKLVTPTPKLRFLNKACI 1271
            +K+  NKS+     QVI  +WKN+HL LPP+  D W++H KGSK V+PT +LRFLNKACI
Sbjct: 1528 IKIKPNKSMLQDFVQVISLSWKNKHLALPPEHLDGWYHHNKGSKFVSPTEQLRFLNKACI 1587

Query: 1272 IRNRETDVDSNGMAKFPW-RLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQA 1331
             R  E D    G+   P   + T+++VEE KA+IRVLPIWSTGI+IA T++Q +F  LQA
Sbjct: 1588 RRIGEPD----GLTIGPKDSICTVQQVEEFKALIRVLPIWSTGIIIAVTVNQHSFPVLQA 1647

Query: 1332 ITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIG 1391
             TMDR +   F+ P+ S+ VFT+LTLTIWVA+YD +I+PL+ K T+R  G T +QRMG G
Sbjct: 1648 DTMDRTLVGDFKIPSGSYGVFTLLTLTIWVAVYDQLIVPLITKITKRPGGLTLQQRMGTG 1707

Query: 1392 LAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQ 1451
            L +S  A+AV+A  E  RR  AI EG+ + P G+V MSAMWL+PQY L GLAE FNAIGQ
Sbjct: 1708 LFLSIAATAVAAITENIRRQHAIDEGLVDTPKGVVNMSAMWLIPQYALIGLAEGFNAIGQ 1767

Query: 1452 IQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHY 1511
            I+F+YSQ P++M SIAVALF+LGM  G+LL ++IV ++ K +   G   W+ NNLNKGHY
Sbjct: 1768 IEFYYSQFPKTMGSIAVALFALGMAFGNLLGSLIVEIVDKVSKHGGKESWVGNNLNKGHY 1827

Query: 1512 DYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVW--DEKEAIEEEG 1546
            DYYYWVLS++   NFLY+L+CSW YG   E     +VW  DE+E++E EG
Sbjct: 1828 DYYYWVLSILCAANFLYFLVCSWAYGPNVE----QKVWNHDEEESMEMEG 1838

BLAST of Lag0034450 vs. NCBI nr
Match: QCD85269.1 (solute carrier family 15 [Vigna unguiculata])

HSP 1 Score: 1634.0 bits (4230), Expect = 0.0e+00
Identity = 862/1551 (55.58%), Postives = 1128/1551 (72.73%), Query Frame = 0

Query: 1    MDDSGNNSSEIGERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCP 60
            M D  N  + IG+  H PPKDFVCPIT +IF DPVTLETGQTYER AI+EW +RGN TCP
Sbjct: 603  MADFDNTLNGIGK--HAPPKDFVCPITSHIFDDPVTLETGQTYERKAIEEWFNRGNFTCP 662

Query: 61   ITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVS---DKALDEANPV----AVLTSPVSV 120
            IT QKLQNTQLPKTNYVLKRLIASW + NP  V    +   ++   V     V TSP SV
Sbjct: 663  ITRQKLQNTQLPKTNYVLKRLIASWKDRNPHLVQPPYESPYEDTEAVVQSTTVSTSPNSV 722

Query: 121  ISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVIN 180
            I+QA+++   +E+R AI NLY SEVL+E+E AVL VE+FW   N+ +DI  ML KP +IN
Sbjct: 723  ITQATVDGMMSELRCAINNLYMSEVLKESEMAVLQVEKFWRGVNLGVDIHRMLAKPAIIN 782

Query: 181  GLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVL 240
            G +EIL NSV  QVL+A VFLL+E+G +D  VI+TLTRV++DV+CI+ LFK+GL EAVVL
Sbjct: 783  GFMEILFNSVEPQVLQAAVFLLAEMGSRDNAVIETLTRVKTDVECIMALFKNGLTEAVVL 842

Query: 241  IYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKE--GSL 300
            +Y L   I SL EM +  SL+ V+  KE ++  M +  K+A V+LL +I G S+E   S 
Sbjct: 843  LYLLNPPITSLAEMAIVESLIMVLNTKEEELVTMCLKPKTAVVLLLARITGSSEEIIASS 902

Query: 301  IAVAVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESF 360
            +   + +ENA  +I+ S  A   +ERIAAV ILLRC++EDG CR  I DKA+L+ ++E+ 
Sbjct: 903  VVNTLFSENAIGTIVSSFGADLAKERIAAVEILLRCMEEDGTCRTNIVDKAELSPLMETL 962

Query: 361  MEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPV 420
            +   + E+F+II F +ELVKLNRRTF EQIL  IK  G  STMH+LLIYLQ A  D  PV
Sbjct: 963  IGADDGERFKIIQFFAELVKLNRRTFTEQILHIIKEEGPFSTMHTLLIYLQAAPHDHCPV 1022

Query: 421  VAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGR 480
            +AGLLLQLD+LVEPRKMSIYREEA+D LI+CL ++DFP  Q++AA+TIMSLQG F   G 
Sbjct: 1023 MAGLLLQLDLLVEPRKMSIYREEAMDTLIACLRNTDFPVTQLAAADTIMSLQGSFDFFGN 1082

Query: 481  PLARYVLLERVGFMKG--HMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHD 540
            PL R VLL+R G  K    + L      NS+P E+E+T E+E+AAD+WER++A+VLVSH+
Sbjct: 1083 PLTREVLLKRAGIDKSSRSLVLVGHQISNSSP-EIEITPEDEKAADDWERRIAYVLVSHE 1142

Query: 541  FGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFAT 600
            FG +FE LA G+KS+ + L SACF+SATWL++ML +LPDTGI  AAR CLL  F++   +
Sbjct: 1143 FGTIFEALADGMKSRNSELRSACFISATWLTYMLTILPDTGIQVAARACLLKQFIAKLNS 1202

Query: 601  TTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGED 660
              D+E++ L +IA+NSF+H PEG   L+S  K+I+RGL+EL+RS PLA +MLK L E  +
Sbjct: 1203 ARDVEDRILSMIALNSFLHFPEGLGDLTSYTKDILRGLRELKRSCPLAPKMLKSLVEENE 1262

Query: 661  SSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEH 720
            S  ++W H+ L + DCSENGEV+S+  FKDKI SGH+DGTIKVW+ +   FHLLQEIQEH
Sbjct: 1263 SKADIWIHKELIKEDCSENGEVVSVTCFKDKIFSGHTDGTIKVWTLKNNSFHLLQEIQEH 1322

Query: 721  SKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFI 780
            +KAVT+L I ES   LYSGSLD+T KVWS+G   I C+QV+D+KDQIHNL+V+ ++ACF+
Sbjct: 1323 TKAVTNLVISESGDTLYSGSLDRTSKVWSIGKSAIHCVQVYDMKDQIHNLIVTNSLACFV 1382

Query: 781  PHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGS 840
            P G GI+V S  GESKLLNSSK+VKCL  V+GKLYCGC DSS+Q + LATGT++ I SG 
Sbjct: 1383 PQGTGIKVQSLSGESKLLNSSKYVKCLAHVNGKLYCGCHDSSVQEIHLATGTVNTIQSGY 1442

Query: 841  RKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSD 900
            ++LL KANP+ A+Q + E +++A +S DG ++KIW+ S+Y+ +GSL T  +VRTM VSS 
Sbjct: 1443 KRLLAKANPIHAIQIHGELIYAAGSSFDGSSIKIWNSSSYNMVGSLQTGSEVRTMAVSSG 1502

Query: 901  LIYLGGR-GVVEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQ-LASP 960
            LIYLG + G VEIW + K  K+DTLQ G NC+V CM L+  EEVLVIGTS G IQ + S 
Sbjct: 1503 LIYLGCKGGTVEIWDKKKSSKVDTLQMGTNCRVNCMALDSNEEVLVIGTSAGQIQYITSF 1562

Query: 961  AMNMC-PEPSHYTMEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNV 1020
               +C P P           K     E+ + ++ V  K+GG RT+P IIANE FEKI+ V
Sbjct: 1563 NYILCVPFPC-------SRRKRVIRMEQNKGSEVVERKRGGYRTIPLIIANETFEKIAIV 1622

Query: 1021 GLHANMIFYLTNEYNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVT 1080
            GL  NMI YL  EY+ + A  AIV+F+WSA TNFLPI  AFLSD +LGRF V+++GT++ 
Sbjct: 1623 GLRVNMILYLLQEYHFEPATAAIVVFLWSAFTNFLPIFCAFLSDCWLGRFSVVALGTLIH 1682

Query: 1081 LLGMVVLWLTAILPKARPPHCKSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAF 1140
            LLG+VVLWLTAI+  ARP   K P   C +A   QL++L+ S  LMAIGAGGIR C+LAF
Sbjct: 1683 LLGLVVLWLTAIIRHARPECEKEP---CANATGSQLLILFSSLTLMAIGAGGIRSCTLAF 1742

Query: 1141 GADQLEKPGDHKNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLML 1200
             ADQ+  P + +N+ TM+SFFNWYYVSVG+SV+IS+ F+VY+Q  AGW+VG+G+ +GLM 
Sbjct: 1743 TADQINNPENPQNESTMKSFFNWYYVSVGLSVTISLTFIVYIQVTAGWIVGFGISMGLMS 1802

Query: 1201 FSTIMFFIGSPLYVKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVTP 1260
            FS ++FF+GS +Y+K+  +KSLF+  AQVI AAWKNRHL LPP+ S+ W++H GS  V P
Sbjct: 1803 FSAVLFFLGSSIYIKVKPDKSLFTGFAQVIAAAWKNRHLPLPPKNSNIWYFHNGSNFVQP 1862

Query: 1261 TPKLRFLNKACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAAT 1320
            T K+RFLNKAC+I+N+E D+DS+GMAK PW + T+++VEELKA+I+VLPIWSTGI+I+ +
Sbjct: 1863 TDKVRFLNKACMIKNKEKDLDSDGMAKEPWSVCTVRQVEELKAIIKVLPIWSTGIIISTS 1922

Query: 1321 IHQFTFAALQAITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSN 1380
            I Q +F+ +QA TM+R +  + + P  +F  F  LTLT+WV +YD I++PLL K      
Sbjct: 1923 ISQQSFSVVQAKTMNR-VVLNMEIPPTNFIAFIFLTLTLWVVLYDRILVPLLPK----ER 1982

Query: 1381 GFTFKQRMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLA 1440
              T KQRMGIGL ISCLA+ VS  +E+KRR+ AI EG  + P G+V MSAMWLVPQ CL 
Sbjct: 1983 VLTVKQRMGIGLVISCLATIVSTLVEKKRRDFAIREGFVDNPKGVVNMSAMWLVPQCCLY 2042

Query: 1441 GLAEAFNAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLG 1500
            GL++  N  GQI+F+YSQ P++M+S+AV+L +LG G G+L+  +IV V+K  T K G   
Sbjct: 2043 GLSQGLNFTGQIEFYYSQFPKTMSSVAVSLCTLGFGVGNLVGCLIVKVVKDGTEKGGKDS 2102

Query: 1501 WLPNNLNKGHYDYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDE 1538
            WL +N+N+GHYDYYY +L ++ +VN L +   S  YG  ++     + WDE
Sbjct: 2103 WLASNINRGHYDYYYGLLFVLNLVNLLCFCTWSRAYGSTQD----IKYWDE 2131

BLAST of Lag0034450 vs. NCBI nr
Match: XP_022933811.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 814/945 (86.14%), Postives = 871/945 (92.17%), Query Frame = 0

Query: 1   MDDSGNNSSEIGERHH-TPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTC 60
           M++SGNN  E GE HH T PKDFVCPITCNIFYDPVT+ETGQTYERSAIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVSDKALDEANPVAVLTSPVSVISQASI 120
           PITGQKLQNTQLPKTNYVLKRLIASWL+ENP+FV+DKALDEA+  AVL SPVSVISQASI
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLKENPNFVTDKALDEADSAAVLISPVSVISQASI 120

Query: 121 NRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEIL 180
           NRS  EVRHAI+N+ SSEVLEEAESAVLCVERFWLEENV+++IQHMLLKPPVINGLVEIL
Sbjct: 121 NRSM-EVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEIL 180

Query: 181 VNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGL 240
           +NSV+LQVLRA++FLLSELGFKD  VIQTLTRVESDVDCIVTLFKSGLMEAVVL+Y+ GL
Sbjct: 181 INSVDLQVLRASIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGL 240

Query: 241 SIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSLIAVAVLAE 300
           SI+ LQEMD+A SLLNVVKKKE DVNKM+++ KSAAV+LLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 SIQRLQEMDLAGSLLNVVKKKE-DVNKMQLNPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQ 360
           NA ESIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IADKADL  IL SFMEVSN+EQ
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADKADLAPILGSFMEVSNDEQ 360

Query: 361 FEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQL 420
           FEII+FLSELVKLNRRTFNEQILQNIK+GGECSTMHSLL+YLQTA RDQ PVVAG LLQL
Sbjct: 361 FEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLLQL 420

Query: 421 DILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLL 480
           DILVEPRK SIYREEA+D+L+SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPL RYVLL
Sbjct: 421 DILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLTRYVLL 480

Query: 481 ERVGFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAK 540
           ERVGF KG MK KRRDN +S PG+VEL+I EERA DEWERKMAFVL SHDFGLLFEPLAK
Sbjct: 481 ERVGFAKGCMKPKRRDNNSSGPGDVELSIAEERATDEWERKMAFVLASHDFGLLFEPLAK 540

Query: 541 GLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALG 600
           GLKSKYAALFSACFVSATWLSHML+VLPDTGIL+ ARVCLLDHF+SIF TTTDIEEKAL 
Sbjct: 541 GLKSKYAALFSACFVSATWLSHMLKVLPDTGILDTARVCLLDHFVSIFITTTDIEEKALA 600

Query: 601 LIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSSTELWSHQV 660
           L+ MNSF+H+PEG Q LSSNMK+IMRGLKELRRST LAFEMLKVLC+GE+SS ELWSHQ 
Sbjct: 601 LLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCKGEESSAELWSHQE 660

Query: 661 LFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIM 720
           LF VDCS NGEVLSIAYFKDKIISGHSDGTIKVWS   T  H LQE QEHSK VTSLAI+
Sbjct: 661 LFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSLAII 720

Query: 721 ESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYS 780
           ESE+KLYSGSLDKTIKVWSLGSD+IQCIQVHDVKDQ+HNLVVSKT+ACFIPHGAGIRVYS
Sbjct: 721 ESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIRVYS 780

Query: 781 WGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPV 840
           WGGESKLLNSSKHVK LNLV GKLYCGC DSSIQ VDLATGT+SYIH GSRKLLGK N V
Sbjct: 781 WGGESKLLNSSKHVKRLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIV 840

Query: 841 QALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GV 900
           Q+LQ YDE+LFSAST+LDG AVKIWSMSN S I +LSTAMD+RTMTVSSDL YLGGR GV
Sbjct: 841 QSLQIYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMTVSSDLTYLGGRGGV 900

Query: 901 VEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQ 944
           VEIWGRDK  KIDTLQTGR CKVACMTLNERE+VLVIGTSDG IQ
Sbjct: 901 VEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQ 943

BLAST of Lag0034450 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 704.1 bits (1816), Expect = 3.4e-201
Identity = 400/976 (40.98%), Postives = 599/976 (61.37%), Query Frame = 0

Query: 18   PPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYV 77
            PPKDFVCPIT  IF DPVTLETGQTYER AIQEWL  GN+TCPIT Q L  + LPKTNYV
Sbjct: 511  PPKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYV 570

Query: 78   LKRLIASWLEENPSFVSD--------------------------KALDEAN--------- 137
            LKRLI SW E+NP    +                          +  D  N         
Sbjct: 571  LKRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQ 630

Query: 138  ------PVAVLTSPVSVISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEE 197
                  P A+ TSP SV+SQA++    N ++  I++L +SE L E E AVL + R   + 
Sbjct: 631  RSNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDS 690

Query: 198  NVEMDIQHMLLKPPVINGLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDV 257
                 I   L KP +INGLVEIL  S N +VLR ++++LSEL F D  V +TL  V+SD 
Sbjct: 691  KTNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDF 750

Query: 258  DCIVTLFKSGLMEAVVLIYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRM--SQKSA 317
            DC+ TL K+GL EA +LIYQL      L   ++  SL++V++ K  +++  ++    K A
Sbjct: 751  DCLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDA 810

Query: 318  AVVLLRKIL--GRSKEGSLIAVAVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDG 377
            A+ +L + L  G     SL A +V++ N   +++  L  +++E R + V +LL C+Q + 
Sbjct: 811  AIAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEK 870

Query: 378  KCRNIIADKADLTLILESFMEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECS 437
             C+N+IA++ +L+ +LE F   ++  +   + FLSELV+LNRRT   QIL  IK+ G  S
Sbjct: 871  SCKNLIANRIELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDEGAFS 930

Query: 438  TMHSLLIYLQTALRDQSPVVAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQ 497
            TMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ LI  L   DF   Q
Sbjct: 931  TMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQ 990

Query: 498  ISAAETIMSLQGRFSTSGRPLARYVLLERVGFMKGHMKLKRRDNGNSAPGEVELTIEEER 557
            + A + ++ L G  S+SG+      LL+  GF + +  L + +       ++  T+E+E+
Sbjct: 991  MKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEK 1050

Query: 558  -AADEWERKMAFVLVSHDFGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGI 617
             A + W++++A VL +H+ G +F+ L + LKS    +  +C V ATWL+HML  LPDTG+
Sbjct: 1051 NALNSWQKRIASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGV 1110

Query: 618  LEAARVCLLDHFMSIFATTTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELR 677
             + AR  LL+  +++  ++ ++EEK L  +A+ +FI +P   ++L    K+I R L+ L+
Sbjct: 1111 RDVARKSLLEEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLK 1170

Query: 678  RSTPLAFEMLKVLCEGED-SSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTI 737
            + + +A +++KV+   +    TELWS + + ++D S NGEVLS+ Y   +++SGH+DGTI
Sbjct: 1171 KYSVVAVDIMKVILNLKSVDVTELWSCKEVVELDLSSNGEVLSMVYLNGQVLSGHTDGTI 1230

Query: 738  KVWSFRETIFHLLQEIQEHSKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVH 797
            KVW  R+ I  ++QE  EH+KAVTSL    S  +LYSGSLDKTI+VW++ SD I+CI V+
Sbjct: 1231 KVWDARKRIPRVIQETHEHTKAVTSLC--SSGDRLYSGSLDKTIRVWTIKSDGIKCIDVY 1290

Query: 798  DVKDQIHNLVVSKTVACFIPHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDS 857
            D+K+ +H L  +  +AC++  G G++V++W    KL+N SK+VK L +   KLYCGC   
Sbjct: 1291 DIKEAVHELAANDKLACYVSQGTGVKVFNWSEAPKLINFSKYVKSLAVAGDKLYCGCSGY 1350

Query: 858  SIQAVDLATGTISYIHSGSRKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYS 917
            SIQ VDL+T T +   +G+RKLLGK   + +LQ +D+ LF+  +S+D  A KI+S+S   
Sbjct: 1351 SIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHDDYLFACGSSVDATAGKIFSLSQKM 1410

Query: 918  AIGSLSTAMDVRTMTVSSDLIYLGGR-GVVEIWGRDKHKKIDTLQ-TGRNCKVACMTLNE 945
             +GSLST +D+  + ++SD I+ G + G +E+W +DK  ++ +++  G + K+  +  + 
Sbjct: 1411 VVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKDKFTRVASIKMAGGHTKITSLVSDV 1470

BLAST of Lag0034450 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 2.3e-197
Identity = 403/977 (41.25%), Postives = 594/977 (60.80%), Query Frame = 0

Query: 18   PPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYV 77
            PPKDFVCPIT  IF DPVTLETGQTYER AIQEWL  GN+TCPIT Q L    LPKTNYV
Sbjct: 513  PPKDFVCPITGQIFSDPVTLETGQTYERKAIQEWLGTGNTTCPITRQALSANILPKTNYV 572

Query: 78   LKRLIASWLEENPSFV----------------------------------SDKALDE--- 137
            LKRLI SW E+NP                                     S K  D+   
Sbjct: 573  LKRLIVSWKEQNPELAQEFSNSNTPRGSSCSPSAKDITMVSSIQRTTDSPSQKYKDDYIR 632

Query: 138  -----ANPVAVLTSPVSVISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLE 197
                    V+V  SP SV+SQA++    N +   IT+L +SE L++ E AVL + R W +
Sbjct: 633  QRNNRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCTSENLQDCEQAVLEIARLWKD 692

Query: 198  ENVEMDIQHMLLKPPVINGLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESD 257
               +  I   L KP V++GLVEIL  S+N +VLR ++++LSEL F D  V +TL  V+SD
Sbjct: 693  SKTDPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYILSELIFSDERVGETLNSVDSD 752

Query: 258  VDCIVTLFKSGLMEAVVLIYQLGLSIESLQEMDMASSLLNVVKKKEGDVN--KMRMSQKS 317
             DC+  L K+GL EA +LIYQL      L E ++  SL+ V++ K  D++  ++ +  K+
Sbjct: 753  FDCLAMLLKNGLAEAALLIYQLRPVFAQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKA 812

Query: 318  AAVVLLRKIL--GRSKEGSLIAVAVLAENATESILDSLKAKQVEERIAAVGILLRCIQED 377
            AA+ +L +IL  G     S+ A +V++ N   +I+  L   + E R   + ILL C+Q +
Sbjct: 813  AAIAILEQILIGGDEYNRSVNASSVISANGIPAIVKYL--DKTEGRRPVISILLCCMQAE 872

Query: 378  GKCRNIIADKADLTLILESFMEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGEC 437
              C++ IA++ +L+ +LE F   ++  +   + FLSELV+LNRRT + Q LQ IK+ G  
Sbjct: 873  KSCKSSIANRIELSPVLELFHAGNDSVRGICVEFLSELVRLNRRTSSNQTLQIIKDEGAF 932

Query: 438  STMHSLLIYLQTALRDQSPVVAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTA 497
            STMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ LI  L   DF   
Sbjct: 933  STMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNN 992

Query: 498  QISAAETIMSLQGRFSTSGRPLARYVLLERVGFMKGH-MKLKRRDNGNSAPGEVELTIEE 557
            Q+ A + ++ L G  ++SG+      LL+  GF + + + +K    G+S    +E   +E
Sbjct: 993  QMKALDALLFLIGHVTSSGKSYTEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDE 1052

Query: 558  ERAADEWERKMAFVLVSHDFGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTG 617
            + A   W++++A VL +H+ G +F+ L + LKS    +  +C V ATWL+HML  LPDTG
Sbjct: 1053 KNAMKSWQKRVASVLCNHENGSIFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTG 1112

Query: 618  ILEAARVCLLDHFMSIFATTTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKEL 677
            + + AR  LL+  M++  ++ ++EEK L  +A+ SFI +P   + L    K+I R L++L
Sbjct: 1113 VRDVARKSLLEALMNVLQSSKNLEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKL 1172

Query: 678  RRSTPLAFEMLKVLCE-GEDSSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGT 737
            ++ + +A ++LK L        TELWS + + ++D S NGEVLS+ Y   +++SGH+DGT
Sbjct: 1173 KKYSTVAADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGHADGT 1232

Query: 738  IKVWSFRETIFHLLQEIQEHSKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQV 797
            IKVW  R+ I  ++QE +EH KAVTSL    S  KLYS SLDKTI+VW++  D I+CI V
Sbjct: 1233 IKVWDARKRIPRVIQETREHKKAVTSLC--SSVDKLYSSSLDKTIRVWTIKPDGIKCIDV 1292

Query: 798  HDVKDQIHNLVVSKTVACFIPHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQD 857
            +DVK+ ++ L  +  +AC++  G G++V++W    K +N +K+VKCL +   KLYCGC  
Sbjct: 1293 YDVKEAVYELAANAKLACYVTQGTGVKVFNWLDAPKFINFNKYVKCLAVSGDKLYCGCSG 1352

Query: 858  SSIQAVDLATGTISYIHSGSRKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNY 917
             SIQ VDL+  T +   +G+RKLLGK   + +LQ +D+ LF+  +S+D  A KI+S+S+ 
Sbjct: 1353 YSIQEVDLSKYTSTSFFTGTRKLLGK-QTIHSLQIHDDLLFACGSSIDATAGKIFSLSSK 1412

Query: 918  SAIGSLSTAMDVRTMTVSSDLIYLGGR-GVVEIWGRDKHKKIDTLQ-TGRNCKVACMTLN 945
              +GSLST +DV  + ++SD I+ G + G +E+W +DK  ++ +++  G N K+  +  +
Sbjct: 1413 MVVGSLSTGLDVHRVAINSDFIFAGTKFGTIEVWLKDKFTRVASIKMAGGNTKITSLASD 1472

BLAST of Lag0034450 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.7e-192
Identity = 393/976 (40.27%), Postives = 587/976 (60.14%), Query Frame = 0

Query: 18   PPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYV 77
            PPKDFVCPIT  IF DPVTLETGQTYER AIQEWL  GN+TCPIT Q L  + LPKTNYV
Sbjct: 511  PPKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYV 570

Query: 78   LKRLIASWLEENPSFVSD--------------------------KALDEAN--------- 137
            LKRLI SW E+NP    +                          +  D  N         
Sbjct: 571  LKRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQ 630

Query: 138  ------PVAVLTSPVSVISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEE 197
                  P A+ TSP SV+SQA++    N ++  I++L +SE L E E AVL + R   + 
Sbjct: 631  RSNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDS 690

Query: 198  NVEMDIQHMLLKPPVINGLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDV 257
                 I   L KP +INGLVEIL  S N +VLR ++++LSEL F D  V +TL  V+SD 
Sbjct: 691  KTNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDF 750

Query: 258  DCIVTLFKSGLMEAVVLIYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRM--SQKSA 317
            DC+ TL K+GL EA +LIYQL      L   ++  SL++V++ K  +++  ++    K A
Sbjct: 751  DCLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDA 810

Query: 318  AVVLLRKIL--GRSKEGSLIAVAVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDG 377
            A+ +L + L  G     SL A +V++ N   +++  L  +++E R + V +LL C+Q + 
Sbjct: 811  AIAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEK 870

Query: 378  KCRNIIADKADLTLILESFMEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECS 437
             C+N+IA++ +L+ +LE F   ++  +   + FLSELV+LNRRT   Q+L  IK+ G  S
Sbjct: 871  SCKNLIANRIELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDEGAFS 930

Query: 438  TMHSLLIYLQTALRDQSPVVAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQ 497
            TMH+ L+YLQ A  +    VA LLLQLD+L EPRKMSIYREEA++ LI  L   DF   Q
Sbjct: 931  TMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQ 990

Query: 498  ISAAETIMSLQGRFSTSGRPLARYVLLERVGFMKGHMKLKRRDNGNSAPGEVELTIEEER 557
            + A + ++ L G  S+SG+      LL+  GF + +  L + +       ++  T+E+E+
Sbjct: 991  MKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEK 1050

Query: 558  -AADEWERKMAFVLVSHDFGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGI 617
             A + W++++A VL +H+ G +F+ L + LKS    +  +C V ATWL+ ML  LPDTG+
Sbjct: 1051 NALNSWQKRIASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGV 1110

Query: 618  LEAARVCLLDHFMSIFATTTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELR 677
             + AR  LL+  + +  ++  +E+  L  +++  FI +P   + L    K+I R L++L+
Sbjct: 1111 RDVARKSLLEEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLK 1170

Query: 678  RSTPLAFEMLKVLCE-GEDSSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTI 737
            + + +A ++LK L        TELWS + + ++D S NGEVLS+ Y   +++SG  DGT 
Sbjct: 1171 KYSTVAADILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGLMDGTS 1230

Query: 738  KVWSFRETIFHLLQEIQEHSKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVH 797
            KV   R+ I  ++QE  EH+KAVTSL    S  +LYS SLDKTI+VW++ SD I+CI V+
Sbjct: 1231 KVCDARKRIPRVIQETHEHTKAVTSLC--SSGDRLYSASLDKTIRVWTIKSDGIKCIDVY 1290

Query: 798  DVKDQIHNLVVSKTVACFIPHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDS 857
            D+K+ +H L  +  +AC++  G G++V++W    KL+N SK+VK L +   KLYCGC   
Sbjct: 1291 DIKEAVHELAANDKLACYVSQGTGVKVFNWSEAPKLINFSKYVKSLAVAGDKLYCGCSGY 1350

Query: 858  SIQAVDLATGTISYIHSGSRKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYS 917
            SIQ VDL+T T +   +G+RKLLGK   + +LQ +D+ LF+  +S+D  A KI+S+S   
Sbjct: 1351 SIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHDDYLFACVSSVDATAGKIFSLSQKM 1410

Query: 918  AIGSLSTAMDVRTMTVSSDLIYLGGR-GVVEIWGRDKHKKIDTLQ-TGRNCKVACMTLNE 945
             +GSLST +D+  + ++SD I+ G + G +E+W +DK  ++ ++Q  G + K+  +  + 
Sbjct: 1411 VVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKDKFTRVASIQMAGGHTKITSLVSDV 1470

BLAST of Lag0034450 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 4.1e-162
Identity = 303/596 (50.84%), Postives = 408/596 (68.46%), Query Frame = 0

Query: 960  MEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNE 1019
            ME  P+E    T  K     +    KGG+ TMPFIIANE FEK+++ GL  NMI YL  +
Sbjct: 1    MENPPNE----TEAKQIQTNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRD 60

Query: 1020 YNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAIL 1079
            Y    AKG  VLFMWSA +NF P+ GAFLSDSYLGRF  ISI ++ + LGMV+LWLTA+L
Sbjct: 61   YRFGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAML 120

Query: 1080 PKARPPHC--KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDH 1139
            P+ +P  C   + G  C S+ A QL LLY +F L++IG+GGIRPCSLAFGADQL+   + 
Sbjct: 121  PQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENP 180

Query: 1140 KNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSP 1199
            KN+R ++SFF WYY S  V+V I+   +VY+Q   GW +G+GVP  LML + ++F + SP
Sbjct: 181  KNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASP 240

Query: 1200 LYVKLMANKSLFSSLAQVIVAAWKNRHLELPP--QTSDKWFYHKGSKLVTPTPKLRFLNK 1259
            LYV     KSLF+ LAQ IVAA+K R L LP    + D +++ K S++  P+ KLRFLNK
Sbjct: 241  LYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNK 300

Query: 1260 ACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAAL 1319
            AC+I NRE ++ S+G A  PWRL T  +VEELKA+I+V+PIWSTGI+++    Q +F  L
Sbjct: 301  ACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLL 360

Query: 1320 QAITMDRHITPH---FQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQ 1379
            QA +MDR ++ H   FQ PA SF +FTI+ L +WV +YD  +IPL +K   R    + K 
Sbjct: 361  QATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKL 420

Query: 1380 RMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAF 1439
            RMG+GL +S LA A+SA +E  RR +AI +G AN    +V +SAMWLVPQY L GLAEA 
Sbjct: 421  RMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEAL 480

Query: 1440 NAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNL 1499
             AIGQ +FFY++ P+SM+SIA +LF LGM   SLLA+++++ + + T++NG   W+ +N+
Sbjct: 481  TAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNI 540

Query: 1500 NKGHYDYYYWVLSLMGVVNFLYYLICSWFYG---DEKEGMEASRVWDEKEAIEEEG 1546
            NKGHY+YYYWVL++M  +N +YY+ICSW YG   D+      + V +E+E I+  G
Sbjct: 541  NKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQVRNGRVNGVREEEELIDIVG 592

BLAST of Lag0034450 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 560.8 bits (1444), Expect = 4.7e-158
Identity = 296/585 (50.60%), Postives = 404/585 (69.06%), Query Frame = 0

Query: 972  SEKMETAQKVAIK---KGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYNVDNAKGA 1031
            +E  ET Q+   +   KGGL TMPFIIANE FEK+++ GL  NMI YL ++Y +   KG 
Sbjct: 8    TESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQ 67

Query: 1032 IVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPKARPPHC- 1091
             VLFMW A TNF+P+ GAFLSDSYLGRF  I I ++ +LLGMVVLWLTA+LP+ +P  C 
Sbjct: 68   TVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCV 127

Query: 1092 KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKNQRTMQSFF 1151
             + G  C SA + QL LLY +F L++IG+GGIRPCSLAFGADQL+   + KN+R ++SFF
Sbjct: 128  ATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFF 187

Query: 1152 NWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLYVKLMANKS 1211
             WYY S  V+V I+   +VY+Q+  GW +G+G+P  LML +  +F   SPLYVK   +KS
Sbjct: 188  GWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKS 247

Query: 1212 LFSSLAQVIVAAWKNRHLELPP--QTSDKWFYHKGSKLVTPTPKLRFLNKACIIRNRETD 1271
            LF+ LAQV+ AA+  R+L LP    + D ++  K S+L  P+ KLRFLNKAC I NR+ D
Sbjct: 248  LFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDED 307

Query: 1272 VDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQAITMDRHIT 1331
            + S+G+A   WRL T  +VE+LKA+++V+P+WSTGI+++  + Q +F  LQA +MDR ++
Sbjct: 308  LGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLS 367

Query: 1332 PH--FQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGLAISCL 1391
             +  FQ PA SF +FTI+ L  WV +YD  I+PL +K   R      K RMG+GL IS L
Sbjct: 368  SNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFL 427

Query: 1392 ASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQIQFFYS 1451
            A AVSA +E  RR  AI +G+AN     V +SAMWLVPQY L GLAEA   IGQ +FFY+
Sbjct: 428  AMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYT 487

Query: 1452 QLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHYDYYYWV 1511
            + P+SM+SIA +LF LGM   ++LA++I++ + K ++K GN+ W+ +N+NKGHYDYYYWV
Sbjct: 488  EFPKSMSSIAASLFGLGMAVANILASVILNAV-KNSSKQGNVSWIEDNINKGHYDYYYWV 547

Query: 1512 LSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEEEGTLN 1549
            L+++  VN +YY++CSW YG   + +   +V   ++  EE   LN
Sbjct: 548  LAILSFVNVIYYVVCSWSYGPTVDQVRNDKVNGMRKEEEEVIKLN 591

BLAST of Lag0034450 vs. ExPASy TrEMBL
Match: A0A6A6LUK5 (RING-type E3 ubiquitin transferase OS=Hevea brasiliensis OX=3981 GN=GH714_024716 PE=3 SV=1)

HSP 1 Score: 1860.5 bits (4818), Expect = 0.0e+00
Identity = 961/1559 (61.64%), Postives = 1204/1559 (77.23%), Query Frame = 0

Query: 1    MDDSGNNSSEIGERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCP 60
            M D+GN     G   HTPPKDFVCPIT ++F DPVTLETGQTYER AIQEWLDRGNSTCP
Sbjct: 383  MGDTGNPP---GAGKHTPPKDFVCPITSHLFDDPVTLETGQTYERKAIQEWLDRGNSTCP 442

Query: 61   ITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVSDKALD---------EANPVAVLTSPV 120
            IT QKL +TQLPKTNYVLKRL+ASW E+NP+F+S+++           ++  +  +TSP 
Sbjct: 443  ITRQKLHSTQLPKTNYVLKRLVASWQEQNPNFISNQSETPHQKTEPSFKSTIMPPITSPN 502

Query: 121  SVISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPV 180
            SVISQA+I+ + +E+RHAITNL  SE+L E+E AVL +E+FW E N++ D+Q ML KPPV
Sbjct: 503  SVISQATIDSTMSELRHAITNLCMSEILNESEMAVLRIEQFWQEANMDPDVQSMLSKPPV 562

Query: 181  INGLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAV 240
            ING VEIL NS++ QVL+AT+FLLSELG +D  VIQTLTRVESDV+CIV LFK GL+EAV
Sbjct: 563  INGFVEILFNSIDPQVLKATIFLLSELGSRDKGVIQTLTRVESDVECIVALFKKGLLEAV 622

Query: 241  VLIYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSL 300
            VLIY L  S  SL EMDM  SLL V+KKKE DV KMR+  K+A+V+LL +IL  S+E ++
Sbjct: 623  VLIYLLRPSTMSLLEMDMVESLLTVIKKKE-DVIKMRLKPKTASVLLLGQILCGSEESTV 682

Query: 301  IAV--AVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILE 360
             ++  A+++    ESI  SL+A   EERIAAVGILLRC+QEDGKCRN IADKA+L  +LE
Sbjct: 683  FSIVNAIVSTKVIESISGSLEADWAEERIAAVGILLRCMQEDGKCRNTIADKAELGPVLE 742

Query: 361  SFMEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQS 420
            +FM  S+ E+FEI+ F SELVKLNRRTFNEQ+L  IK+ G  STMHSLL YLQTAL+DQ 
Sbjct: 743  TFMSASDGERFEIVHFFSELVKLNRRTFNEQVLHIIKDEGAFSTMHSLLNYLQTALQDQC 802

Query: 421  PVVAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTS 480
            PVVAGLLLQLD+LVEPRKMSIYREEAID LISCLG  +FP AQI+AA TIMSLQGRF+ S
Sbjct: 803  PVVAGLLLQLDLLVEPRKMSIYREEAIDNLISCLGKYEFPVAQIAAAHTIMSLQGRFTAS 862

Query: 481  GRPLARYVLLERVGFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHD 540
            G+ L R  LL+R G  K +  L R D   +  GE+E T+EEERAA+EWERKMAF LVSH+
Sbjct: 863  GKSLGRAFLLKRAGHSKSYRYLMRMDQLGNLSGEIEETLEEERAAEEWERKMAFALVSHE 922

Query: 541  FGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFAT 600
            FGL+FE LA+GLKS+ A L SACFVSATWL +ML VLPDTGI  AARVCLL  F++IF +
Sbjct: 923  FGLVFEALAEGLKSRNAELCSACFVSATWLVYMLGVLPDTGIRGAARVCLLKRFVTIFKS 982

Query: 601  TTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGED 660
              D E++ L L+A+ SFI++PEG + L+S +K+I +GL+EL++S+PLA E+LKVL EG +
Sbjct: 983  AKDTEDRVLSLLALKSFINDPEGLRDLTSYVKDIKKGLRELKKSSPLALEVLKVLSEGHE 1042

Query: 661  SSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEH 720
            SS E W+H+ L QVDCS+NGEVLSI  FKDKI SGHSDGTIKVW+ R +I  L+QEI+EH
Sbjct: 1043 SSAEFWNHEELTQVDCSDNGEVLSITCFKDKIFSGHSDGTIKVWTGRGSILQLIQEIREH 1102

Query: 721  SKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFI 780
            +KAVTSL ++ S   LYSGSLD+T +VWS+G++ + C++VHD+KDQ+HNLVV+ +++CFI
Sbjct: 1103 TKAVTSLVVLHSGDSLYSGSLDRTARVWSIGNEALHCVKVHDMKDQVHNLVVANSISCFI 1162

Query: 781  PHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGS 840
            P GAG++V+SW G  K LNS+K+VKCL+LV GKLYCGC DSSIQ +DLAT T   I +GS
Sbjct: 1163 PQGAGVKVHSWNGGFKFLNSNKYVKCLSLVQGKLYCGCHDSSIQEIDLATVTSVTIQNGS 1222

Query: 841  RKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSD 900
            RKLLGKANP+ AL   D  ++SAS++LDG AVKIWS S Y  +GS+ T ++VR M +SS+
Sbjct: 1223 RKLLGKANPIHALHVRDGLIYSASSALDGAAVKIWSASTYGLVGSVPTTLEVRAMAISSE 1282

Query: 901  LIYLGGR-GVVEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPA 960
            LIYLG + G VEIW + K  +I+TLQTG + +V C+ L+  EEVLVIGTSDG IQ     
Sbjct: 1283 LIYLGSKGGTVEIWDQKKQNRIETLQTGTDGRVLCIALDGNEEVLVIGTSDGRIQ----- 1342

Query: 961  MNMCPEPSHYTMEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGL 1020
                             + METTS++  T Q V  KKGGLRTMPFIIANE FEK++ VGL
Sbjct: 1343 ---------------EKQGMETTSDERNTIQNVKRKKGGLRTMPFIIANETFEKVAGVGL 1402

Query: 1021 HANMIFYLTNEYNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLL 1080
            HANMI YL NEYN+ +A GA +LF+W+A + F PI GAF+SDSYLGRF+VI +GTVV+LL
Sbjct: 1403 HANMILYLQNEYNLSSATGANILFIWAATSYFTPIIGAFVSDSYLGRFRVIVLGTVVSLL 1462

Query: 1081 GMVVLWLTAILPKARPPHCKSPG---EFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLA 1140
            GM++LWLTAI+P ARP HC       + C S +A Q+M L  SF LMA+GAGGIRPCSLA
Sbjct: 1463 GMILLWLTAIIPSARPLHCDQKDGNLQGCASPNAAQMMFLLSSFALMAVGAGGIRPCSLA 1522

Query: 1141 FGADQLEKPGDHKNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLM 1200
            FGADQL+ P + KN +T+QSFFNWYY SVG+SV +SVIF+V +Q+AAGWVVG+ VPVG M
Sbjct: 1523 FGADQLDNPENPKNDKTLQSFFNWYYASVGISVMVSVIFVVAIQDAAGWVVGFAVPVGFM 1582

Query: 1201 LFSTIMFFIGSPLYVKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVT 1260
            L S ++F +GS  YVK+  N SL +  AQVI AAWKN+++ LP   SD+W++HK S LV 
Sbjct: 1583 LLSIVLFLMGSSRYVKVKPNTSLLTGFAQVISAAWKNKYISLPRSDSDRWYHHKDSTLVI 1642

Query: 1261 PTPKLRFLNKACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAA 1320
            PT KL FLNKAC+I N E D+D  G+A   W+L T+K+VEELKA+I+V+PIWSTGI+++A
Sbjct: 1643 PTEKLSFLNKACVIINPEKDLDKEGLAIDAWKLCTVKQVEELKALIKVMPIWSTGIMLSA 1702

Query: 1321 TIHQFTFAALQAITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRS 1380
            T+ Q  F  LQA+TMDRH+    + PAASF VFTILTLTIWVAIYD I++P +AKFT+R 
Sbjct: 1703 TLSQHAFPVLQALTMDRHLVGKVKIPAASFGVFTILTLTIWVAIYDRILVPRIAKFTKRP 1762

Query: 1381 NGFTFKQRMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCL 1440
             G + KQRMGIGL +SC+++A++  +E KRR  AI EG+A+ P G+V MSAMWLVPQ+C+
Sbjct: 1763 RGLSNKQRMGIGLLLSCISTAIAGAVEHKRRAMAIREGLAHKPMGLVNMSAMWLVPQHCV 1822

Query: 1441 AGLAEAFNAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNL 1500
             GLAEAFNAIGQI+F+YSQ P++M+SI VALFSLGM  G+LL + IVS++K+ET KNG +
Sbjct: 1823 TGLAEAFNAIGQIEFYYSQFPKTMSSIGVALFSLGMAFGNLLGSFIVSILKRETGKNGKV 1882

Query: 1501 GWLPNNLNKGHYDYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEEE 1545
             W+ NNLN+GHYDYYYW+L+ + V+NF YYL+CS FYG E      +R+WD   AIEE+
Sbjct: 1883 SWVANNLNRGHYDYYYWLLASLSVLNFFYYLLCSRFYGSEN-----NRIWD--AAIEEK 1910

BLAST of Lag0034450 vs. ExPASy TrEMBL
Match: A0A4S4E4V3 (RING-type E3 ubiquitin transferase OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_024355 PE=3 SV=1)

HSP 1 Score: 1827.4 bits (4732), Expect = 0.0e+00
Identity = 937/1539 (60.88%), Postives = 1185/1539 (77.00%), Query Frame = 0

Query: 12   GERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQL 71
            G   HTPPKDFVCPIT NIF DPVTLETGQTYER AIQEWL+RGN TCPIT QKL  TQL
Sbjct: 413  GGEKHTPPKDFVCPITSNIFGDPVTLETGQTYERKAIQEWLERGNCTCPITRQKLHRTQL 472

Query: 72   PKTNYVLKRLIASWLEENPSFV---SDKALDEANPV----AVLTSPVSVISQASINRSTN 131
            PKTNYVLKRLIASW E+NP+ +   S+ +  E+ PV       TSP SVISQA+I+ + +
Sbjct: 473  PKTNYVLKRLIASWQEQNPTSIPVQSENSQPESKPVFNSMMRSTSPNSVISQATIDGTVS 532

Query: 132  EVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEILVNSVN 191
            E+   ITNL  SE+L+E+E AVL +ERFW E N+E++IQ ML KPPVING VEIL NSV+
Sbjct: 533  ELHLVITNLCMSEILKESEMAVLQIERFWQEANMEVEIQAMLSKPPVINGFVEILFNSVD 592

Query: 192  LQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGLSIESL 251
             QVL AT+FLLSELG +D  VIQTLTRV+SDV+C+V LF +GL+E VVLIY L  S  SL
Sbjct: 593  PQVLTATIFLLSELGSRDGAVIQTLTRVDSDVECVVALFTNGLLETVVLIYLLRPSSISL 652

Query: 252  QEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSL--IAVAVLAENAT 311
             +MDM  +LL V++ KE D+ KM M  K+AAV+LL +I G ++E SL  I   V+     
Sbjct: 653  IKMDMVDTLLTVIRNKEDDMIKMCMKPKTAAVLLLGQIFGSAEEISLSEITRRVIMGKVI 712

Query: 312  ESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQFEI 371
            ESI+ SL++   EERIAAVGILLRC+Q +GKCRN IADKA+L  +LE F   S+ E+FEI
Sbjct: 713  ESIVASLQSDWAEERIAAVGILLRCMQGEGKCRNSIADKAELAPVLECFTGASDGERFEI 772

Query: 372  ILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQLDIL 431
            + FL ELVKLNRRTFNEQIL  IK+ G  STMH+LLIYLQTAL+DQ P+VAGLLLQLD+L
Sbjct: 773  VHFLFELVKLNRRTFNEQILHIIKDEGTLSTMHTLLIYLQTALQDQCPIVAGLLLQLDLL 832

Query: 432  VEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 491
             EPRKMSIYREEAID LISCL +SDFP AQI+AAETI++LQGRFS+SG+PLAR  LL+  
Sbjct: 833  EEPRKMSIYREEAIDTLISCLRNSDFPAAQIAAAETILALQGRFSSSGKPLARAFLLKHG 892

Query: 492  GFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAKGLK 551
            G  K H  + R++  ++  GE++ ++EEE+AA+EWERKMAFVLVSH+FGL+FE L +GLK
Sbjct: 893  GVDKSHKYVMRKEQLSTMSGEIQESLEEEKAAEEWERKMAFVLVSHEFGLVFEALGEGLK 952

Query: 552  SKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALGLIA 611
            S+YA L SACFVSA WL HML VLPDTG+  AARVCLL  F+S   +  D E+K L ++A
Sbjct: 953  SRYAELSSACFVSAAWLVHMLNVLPDTGLRGAARVCLLKCFVSTLKSARDTEDKVLSMLA 1012

Query: 612  MNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSST-ELWSHQVLF 671
            +NSF+H+PEG + ++ +MK+I++GL+EL++S+ +A +MLKV  E  DSST +LW+H+ L 
Sbjct: 1013 LNSFVHDPEGLRDITYHMKDILKGLRELKKSSTMALDMLKVFAEERDSSTQDLWNHKDLV 1072

Query: 672  QVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIMES 731
            Q DCS NGEVLS+ YF+DKI SGHSDGTIKVW+ + +I HL+QE ++H+KAV+SLAI++S
Sbjct: 1073 QEDCSVNGEVLSVVYFRDKIFSGHSDGTIKVWTGKGSILHLIQETRKHTKAVSSLAILQS 1132

Query: 732  EQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYSWG 791
              KLYSGSLDKT++VWS+G +++ C QVHD+KDQ+HNLVV+ +VACFIP GAGI+++SWG
Sbjct: 1133 GDKLYSGSLDKTVRVWSIGGEVMHCTQVHDMKDQVHNLVVANSVACFIPQGAGIKMHSWG 1192

Query: 792  GESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPVQA 851
            G SKLLN +K  KC+ L+HGKLY GCQD+SIQ +DLATGT++ I SGSRKLLGKANP+ A
Sbjct: 1193 GGSKLLNPNKSAKCITLLHGKLYSGCQDNSIQEIDLATGTLNTIQSGSRKLLGKANPIHA 1252

Query: 852  LQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GVVE 911
            L   D  L+SAS+SL+G AVKIW+ SNYS +GSL   ++VR M VSS+LIYLG + G VE
Sbjct: 1253 LNIRDGLLYSASSSLNGAAVKIWNASNYSMVGSLPLTLEVRAMVVSSELIYLGCKGGTVE 1312

Query: 912  IWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPAMNMCPEPSHYTM 971
            +W R KH ++D LQTG N +V CM L+  EEVLVIGTSDG                   M
Sbjct: 1313 VWCRKKHNRVDMLQTGSNGRVLCMALDADEEVLVIGTSDG------------------RM 1372

Query: 972  EKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEY 1031
            E+  ++ ++TTS      Q V+ +KGGLRTMPF+IANE  EK+++VGLHANMI YL NEY
Sbjct: 1373 EERSADPVKTTS------QPVSRRKGGLRTMPFVIANEALEKVASVGLHANMILYLKNEY 1432

Query: 1032 NVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILP 1091
            ++    G  +LF+W+A++NFLP  GAFLSDSYLGRF V+ +G++VTLLG+VVLW TAI  
Sbjct: 1433 HLSIVTGTSILFLWNAVSNFLPTFGAFLSDSYLGRFCVVGLGSIVTLLGLVVLWFTAIFK 1492

Query: 1092 KARPPHCKSPG-EFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKN 1151
             ARPPHC  P  + C S    QL LL+ SF+LMAIGAGGIRPCSLAFGADQ  KP + KN
Sbjct: 1493 HARPPHCDHPNTDHCASPKPAQLALLFSSFVLMAIGAGGIRPCSLAFGADQFNKPDNPKN 1552

Query: 1152 QRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLY 1211
             R +Q+FFNWYYVS+G+S+ ISV  +VY+Q A GW VG+GVPV LM FST+MFF+GSPL+
Sbjct: 1553 TRVLQTFFNWYYVSLGISIMISVTVIVYIQTAKGWTVGFGVPVVLMFFSTVMFFLGSPLF 1612

Query: 1212 VKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYH-KGSKLVTPTPKLRFLNKACI 1271
            VK+ ANKSL +  AQV+  +WKN+HL+ PP+ S+  ++H KGSKL+ P  KLRFLNKACI
Sbjct: 1613 VKVKANKSLVTGFAQVMAVSWKNKHLDFPPKDSEGQYHHKKGSKLLAPAEKLRFLNKACI 1672

Query: 1272 IRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQAI 1331
            I+N E D++ +G A  PW L T+++VEELKA+I+VLPIWS GI+IA TI Q  F  LQA 
Sbjct: 1673 IKNPEKDLNPDGTALDPWTLCTVQQVEELKALIKVLPIWSAGIIIAVTISQQAFPVLQAY 1732

Query: 1332 TMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGL 1391
            TMDRH   +F+ P  S+ VF+ILTLTIWVAIYD +I+P L+K+T+R  G + KQRMGIGL
Sbjct: 1733 TMDRHFNKNFKMPPGSYGVFSILTLTIWVAIYDRLIVPWLSKYTKRPRGLSLKQRMGIGL 1792

Query: 1392 AISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQI 1451
             +SC+A+ V+A IE  RR RAI EG+A+ P  +V MSAMWLVPQ+CL G++EAFNAIGQI
Sbjct: 1793 FLSCIATTVAALIESTRRARAINEGLADQPDAVVNMSAMWLVPQHCLVGISEAFNAIGQI 1852

Query: 1452 QFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHYD 1511
            QF+YSQ P+SM+SI +ALF+LGMG G+LL ++IVS++   T + GN+ W+ NNLN+GHYD
Sbjct: 1853 QFYYSQFPKSMSSIGIALFTLGMGVGNLLGSLIVSILAHATKRGGNVSWVSNNLNRGHYD 1912

Query: 1512 YYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDE 1538
            YYYW+L+ + V NF Y+L+C W YG E++      VWD+
Sbjct: 1913 YYYWILTCLSVANFFYFLLCGWAYGSEEQ-----NVWDD 1922

BLAST of Lag0034450 vs. ExPASy TrEMBL
Match: A0A175YK85 (RING-type E3 ubiquitin transferase OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_028695 PE=3 SV=1)

HSP 1 Score: 1666.4 bits (4314), Expect = 0.0e+00
Identity = 883/1550 (56.97%), Postives = 1129/1550 (72.84%), Query Frame = 0

Query: 12   GERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQL 71
            G   H  P+DFVCPIT  IF DPVTLETGQTYER AIQEW+DRGN TCPIT QKL  TQL
Sbjct: 328  GGGKHKAPRDFVCPITSYIFDDPVTLETGQTYERKAIQEWIDRGNLTCPITRQKLNGTQL 387

Query: 72   PKTNYVLKRLIASWLEENPS---FVSDKALDEANPV----AVLTSPVSVISQASINRSTN 131
            P TNYVLKRLIA W E+N S     SD    E+ P     A L SP SVISQA+ + +  
Sbjct: 388  PNTNYVLKRLIARWREQNSSPTPVKSDNQHQESEPSFSNGAPLASPDSVISQATFDGAVG 447

Query: 132  EVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEILVNSVN 191
            E+RHAI  L  SE+L+E+E AVL +E+FW E N++ DIQ ML +P +ING V+IL NSV+
Sbjct: 448  ELRHAIETLSMSEILKESEMAVLWIEKFWKEANMQADIQTMLSEPSIINGFVDILFNSVD 507

Query: 192  LQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGLSIESL 251
             QVLRA+VFLLSELG +D  VIQTL  V+ DV+C+V LF+ GL+EAVVL+Y L  S  S 
Sbjct: 508  AQVLRASVFLLSELGSRDDSVIQTLCGVDPDVECVVALFRKGLLEAVVLVYLLRSSATSF 567

Query: 252  QEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSLIAV--AVLAENAT 311
             ++ +  SLLNV+ KKE D  KM +  +SA+V+LL++ILG + E S+     + +++   
Sbjct: 568  IDVGILDSLLNVLGKKEDDFVKMCIRPQSASVLLLKQILGSADEISISGALSSFISDEVI 627

Query: 312  ESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQFEI 371
            E+IL SLKA+   ER AAV ILLRCIQEDG  RN+IA KA+L  ++ESF+E + +E+F+I
Sbjct: 628  ENILYSLKAEWHGERCAAVSILLRCIQEDGNRRNLIAQKAELAPLIESFLEANEKERFQI 687

Query: 372  ILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQLDIL 431
            + FLSELVKL+RR  NE IL  IK+ G  STMH+LLIYLQTA +DQ PVVAGLLLQLDIL
Sbjct: 688  VQFLSELVKLHRRIDNENILHVIKDEGTFSTMHTLLIYLQTARQDQRPVVAGLLLQLDIL 747

Query: 432  VEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLLERV 491
            ++PRK SIYREEA+D +ISCL +SDFP AQI+AAETI+ LQGRFS SG+ LAR +LL R 
Sbjct: 748  MKPRKASIYREEAVDTIISCLRNSDFPDAQIAAAETILVLQGRFSYSGKSLARDLLLRRA 807

Query: 492  GFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAKGLK 551
            G  K +     +D            +EEE AA+EWE+K+AF LVSH+FGLLFE LA+GLK
Sbjct: 808  GLHKNYKSQMLKDQDMMTSQNSGEMMEEENAAEEWEKKVAFALVSHEFGLLFEALAEGLK 867

Query: 552  SKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALGLIA 611
            S  A L SACFVSATWL  ML  LPDTGI  AAR CLL+ F+SIF +  DIE++A+ ++A
Sbjct: 868  STNAKLHSACFVSATWLVDMLARLPDTGIRGAARACLLEQFVSIFKSAKDIEDRAVSMLA 927

Query: 612  MNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSSTELWSHQVLFQ 671
            ++SFIHEPEG Q L+  MK+I++GL+EL++ + +AFEMLK L +G+DSS E+W+H+ L Q
Sbjct: 928  LSSFIHEPEGLQDLTLYMKDILKGLRELKKFSVVAFEMLKSLADGDDSSNEMWNHKELVQ 987

Query: 672  VDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIMESE 731
             DCS NGEVLS+  FKDKI SGHSDGTIK W+ + +I HL+QE +EH+KAVTSL + ES 
Sbjct: 988  EDCSANGEVLSVVCFKDKIFSGHSDGTIKAWTGKGSILHLIQETREHTKAVTSLTVSESG 1047

Query: 732  QKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYSWGG 791
             KLYSGSLDKT++ W++ S+ +QC  V ++KD ++NL ++  ++C+IP+GAGI+V+S  G
Sbjct: 1048 DKLYSGSLDKTVRAWNVDSEGMQCEHVSEMKDHVNNLAIANNISCYIPNGAGIKVHSLNG 1107

Query: 792  ESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPVQAL 851
             SKLLN  K+VKCL LV GKL+CGC D+SIQ +DL TG +  I SGSR  + KANP+ AL
Sbjct: 1108 TSKLLNPKKYVKCLVLVQGKLFCGCHDNSIQEIDLETGALCTIQSGSRHFITKANPIYAL 1167

Query: 852  QAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GVVEI 911
            Q +D  ++SAS+ LDG AVKIWS S Y  IGS+ + ++VR+M V+SDLIYLG + G+VE+
Sbjct: 1168 QVHDGLIYSASSPLDGAAVKIWSASTYDIIGSVPSTLEVRSMEVTSDLIYLGCKGGIVEV 1227

Query: 912  WGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQLASPAMNMCPEPSHYTME 971
            W R K  K +TLQTG N KV CM L+  EEVLVIGTS+G IQ+                 
Sbjct: 1228 WCRKKLDKKETLQTGTNGKVLCMALDSDEEVLVIGTSNGRIQM----------------- 1287

Query: 972  KYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYN 1031
            K+ SE              V  K GGLRTMPFIIANE FEK+++ GLHANMI YL  EY+
Sbjct: 1288 KFSSE-------------IVTRKLGGLRTMPFIIANEAFEKVASTGLHANMILYLVFEYH 1347

Query: 1032 VDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPK 1091
            +D   G  +LF+W+A+ NF+PI GAFLSDSYLGRF+VI+ GTVVTL+G ++LW TAI+P+
Sbjct: 1348 MDVVTGTTILFLWNAIANFMPILGAFLSDSYLGRFRVIAWGTVVTLIGTIILWFTAIIPE 1407

Query: 1092 ARPPHC--KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKN 1151
              PP C  + P + CV  D+GQL LL+ S  +MAIGAGGIRPCSLAFGADQ +KP + +N
Sbjct: 1408 FTPPDCDIRQPRD-CVKPDSGQLALLFISLAVMAIGAGGIRPCSLAFGADQFDKPDNPEN 1467

Query: 1152 QRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLY 1211
            +R +Q FFN YY SVG+S+ ISV  +VY+QN  GW+VG+GVPVG +L ST++F IGSPL+
Sbjct: 1468 ERILQRFFNLYYASVGISLMISVTVIVYIQNKFGWIVGFGVPVGCLLLSTVLFLIGSPLF 1527

Query: 1212 VKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYH-KGSKLVTPTPKLRFLNKACI 1271
            +K+  NKS+     QVI  +WKN+HL LPP+  D W++H KGSK V+PT +LRFLNKACI
Sbjct: 1528 IKIKPNKSMLQDFVQVISLSWKNKHLALPPEHLDGWYHHNKGSKFVSPTEQLRFLNKACI 1587

Query: 1272 IRNRETDVDSNGMAKFPW-RLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQA 1331
             R  E D    G+   P   + T+++VEE KA+IRVLPIWSTGI+IA T++Q +F  LQA
Sbjct: 1588 RRIGEPD----GLTIGPKDSICTVQQVEEFKALIRVLPIWSTGIIIAVTVNQHSFPVLQA 1647

Query: 1332 ITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIG 1391
             TMDR +   F+ P+ S+ VFT+LTLTIWVA+YD +I+PL+ K T+R  G T +QRMG G
Sbjct: 1648 DTMDRTLVGDFKIPSGSYGVFTLLTLTIWVAVYDQLIVPLITKITKRPGGLTLQQRMGTG 1707

Query: 1392 LAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQ 1451
            L +S  A+AV+A  E  RR  AI EG+ + P G+V MSAMWL+PQY L GLAE FNAIGQ
Sbjct: 1708 LFLSIAATAVAAITENIRRQHAIDEGLVDTPKGVVNMSAMWLIPQYALIGLAEGFNAIGQ 1767

Query: 1452 IQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHY 1511
            I+F+YSQ P++M SIAVALF+LGM  G+LL ++IV ++ K +   G   W+ NNLNKGHY
Sbjct: 1768 IEFYYSQFPKTMGSIAVALFALGMAFGNLLGSLIVEIVDKVSKHGGKESWVGNNLNKGHY 1827

Query: 1512 DYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVW--DEKEAIEEEG 1546
            DYYYWVLS++   NFLY+L+CSW YG   E     +VW  DE+E++E EG
Sbjct: 1828 DYYYWVLSILCAANFLYFLVCSWAYGPNVE----QKVWNHDEEESMEMEG 1838

BLAST of Lag0034450 vs. ExPASy TrEMBL
Match: A0A4D6L9S4 (RING-type E3 ubiquitin transferase OS=Vigna unguiculata OX=3917 GN=DEO72_LG2g5629 PE=3 SV=1)

HSP 1 Score: 1634.0 bits (4230), Expect = 0.0e+00
Identity = 862/1551 (55.58%), Postives = 1128/1551 (72.73%), Query Frame = 0

Query: 1    MDDSGNNSSEIGERHHTPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTCP 60
            M D  N  + IG+  H PPKDFVCPIT +IF DPVTLETGQTYER AI+EW +RGN TCP
Sbjct: 603  MADFDNTLNGIGK--HAPPKDFVCPITSHIFDDPVTLETGQTYERKAIEEWFNRGNFTCP 662

Query: 61   ITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVS---DKALDEANPV----AVLTSPVSV 120
            IT QKLQNTQLPKTNYVLKRLIASW + NP  V    +   ++   V     V TSP SV
Sbjct: 663  ITRQKLQNTQLPKTNYVLKRLIASWKDRNPHLVQPPYESPYEDTEAVVQSTTVSTSPNSV 722

Query: 121  ISQASINRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVIN 180
            I+QA+++   +E+R AI NLY SEVL+E+E AVL VE+FW   N+ +DI  ML KP +IN
Sbjct: 723  ITQATVDGMMSELRCAINNLYMSEVLKESEMAVLQVEKFWRGVNLGVDIHRMLAKPAIIN 782

Query: 181  GLVEILVNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVL 240
            G +EIL NSV  QVL+A VFLL+E+G +D  VI+TLTRV++DV+CI+ LFK+GL EAVVL
Sbjct: 783  GFMEILFNSVEPQVLQAAVFLLAEMGSRDNAVIETLTRVKTDVECIMALFKNGLTEAVVL 842

Query: 241  IYQLGLSIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKE--GSL 300
            +Y L   I SL EM +  SL+ V+  KE ++  M +  K+A V+LL +I G S+E   S 
Sbjct: 843  LYLLNPPITSLAEMAIVESLIMVLNTKEEELVTMCLKPKTAVVLLLARITGSSEEIIASS 902

Query: 301  IAVAVLAENATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESF 360
            +   + +ENA  +I+ S  A   +ERIAAV ILLRC++EDG CR  I DKA+L+ ++E+ 
Sbjct: 903  VVNTLFSENAIGTIVSSFGADLAKERIAAVEILLRCMEEDGTCRTNIVDKAELSPLMETL 962

Query: 361  MEVSNEEQFEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPV 420
            +   + E+F+II F +ELVKLNRRTF EQIL  IK  G  STMH+LLIYLQ A  D  PV
Sbjct: 963  IGADDGERFKIIQFFAELVKLNRRTFTEQILHIIKEEGPFSTMHTLLIYLQAAPHDHCPV 1022

Query: 421  VAGLLLQLDILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGR 480
            +AGLLLQLD+LVEPRKMSIYREEA+D LI+CL ++DFP  Q++AA+TIMSLQG F   G 
Sbjct: 1023 MAGLLLQLDLLVEPRKMSIYREEAMDTLIACLRNTDFPVTQLAAADTIMSLQGSFDFFGN 1082

Query: 481  PLARYVLLERVGFMKG--HMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHD 540
            PL R VLL+R G  K    + L      NS+P E+E+T E+E+AAD+WER++A+VLVSH+
Sbjct: 1083 PLTREVLLKRAGIDKSSRSLVLVGHQISNSSP-EIEITPEDEKAADDWERRIAYVLVSHE 1142

Query: 541  FGLLFEPLAKGLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFAT 600
            FG +FE LA G+KS+ + L SACF+SATWL++ML +LPDTGI  AAR CLL  F++   +
Sbjct: 1143 FGTIFEALADGMKSRNSELRSACFISATWLTYMLTILPDTGIQVAARACLLKQFIAKLNS 1202

Query: 601  TTDIEEKALGLIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGED 660
              D+E++ L +IA+NSF+H PEG   L+S  K+I+RGL+EL+RS PLA +MLK L E  +
Sbjct: 1203 ARDVEDRILSMIALNSFLHFPEGLGDLTSYTKDILRGLRELKRSCPLAPKMLKSLVEENE 1262

Query: 661  SSTELWSHQVLFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEH 720
            S  ++W H+ L + DCSENGEV+S+  FKDKI SGH+DGTIKVW+ +   FHLLQEIQEH
Sbjct: 1263 SKADIWIHKELIKEDCSENGEVVSVTCFKDKIFSGHTDGTIKVWTLKNNSFHLLQEIQEH 1322

Query: 721  SKAVTSLAIMESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFI 780
            +KAVT+L I ES   LYSGSLD+T KVWS+G   I C+QV+D+KDQIHNL+V+ ++ACF+
Sbjct: 1323 TKAVTNLVISESGDTLYSGSLDRTSKVWSIGKSAIHCVQVYDMKDQIHNLIVTNSLACFV 1382

Query: 781  PHGAGIRVYSWGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGS 840
            P G GI+V S  GESKLLNSSK+VKCL  V+GKLYCGC DSS+Q + LATGT++ I SG 
Sbjct: 1383 PQGTGIKVQSLSGESKLLNSSKYVKCLAHVNGKLYCGCHDSSVQEIHLATGTVNTIQSGY 1442

Query: 841  RKLLGKANPVQALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSD 900
            ++LL KANP+ A+Q + E +++A +S DG ++KIW+ S+Y+ +GSL T  +VRTM VSS 
Sbjct: 1443 KRLLAKANPIHAIQIHGELIYAAGSSFDGSSIKIWNSSSYNMVGSLQTGSEVRTMAVSSG 1502

Query: 901  LIYLGGR-GVVEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQ-LASP 960
            LIYLG + G VEIW + K  K+DTLQ G NC+V CM L+  EEVLVIGTS G IQ + S 
Sbjct: 1503 LIYLGCKGGTVEIWDKKKSSKVDTLQMGTNCRVNCMALDSNEEVLVIGTSAGQIQYITSF 1562

Query: 961  AMNMC-PEPSHYTMEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNV 1020
               +C P P           K     E+ + ++ V  K+GG RT+P IIANE FEKI+ V
Sbjct: 1563 NYILCVPFPC-------SRRKRVIRMEQNKGSEVVERKRGGYRTIPLIIANETFEKIAIV 1622

Query: 1021 GLHANMIFYLTNEYNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVT 1080
            GL  NMI YL  EY+ + A  AIV+F+WSA TNFLPI  AFLSD +LGRF V+++GT++ 
Sbjct: 1623 GLRVNMILYLLQEYHFEPATAAIVVFLWSAFTNFLPIFCAFLSDCWLGRFSVVALGTLIH 1682

Query: 1081 LLGMVVLWLTAILPKARPPHCKSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAF 1140
            LLG+VVLWLTAI+  ARP   K P   C +A   QL++L+ S  LMAIGAGGIR C+LAF
Sbjct: 1683 LLGLVVLWLTAIIRHARPECEKEP---CANATGSQLLILFSSLTLMAIGAGGIRSCTLAF 1742

Query: 1141 GADQLEKPGDHKNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLML 1200
             ADQ+  P + +N+ TM+SFFNWYYVSVG+SV+IS+ F+VY+Q  AGW+VG+G+ +GLM 
Sbjct: 1743 TADQINNPENPQNESTMKSFFNWYYVSVGLSVTISLTFIVYIQVTAGWIVGFGISMGLMS 1802

Query: 1201 FSTIMFFIGSPLYVKLMANKSLFSSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVTP 1260
            FS ++FF+GS +Y+K+  +KSLF+  AQVI AAWKNRHL LPP+ S+ W++H GS  V P
Sbjct: 1803 FSAVLFFLGSSIYIKVKPDKSLFTGFAQVIAAAWKNRHLPLPPKNSNIWYFHNGSNFVQP 1862

Query: 1261 TPKLRFLNKACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAAT 1320
            T K+RFLNKAC+I+N+E D+DS+GMAK PW + T+++VEELKA+I+VLPIWSTGI+I+ +
Sbjct: 1863 TDKVRFLNKACMIKNKEKDLDSDGMAKEPWSVCTVRQVEELKAIIKVLPIWSTGIIISTS 1922

Query: 1321 IHQFTFAALQAITMDRHITPHFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSN 1380
            I Q +F+ +QA TM+R +  + + P  +F  F  LTLT+WV +YD I++PLL K      
Sbjct: 1923 ISQQSFSVVQAKTMNR-VVLNMEIPPTNFIAFIFLTLTLWVVLYDRILVPLLPK----ER 1982

Query: 1381 GFTFKQRMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLA 1440
              T KQRMGIGL ISCLA+ VS  +E+KRR+ AI EG  + P G+V MSAMWLVPQ CL 
Sbjct: 1983 VLTVKQRMGIGLVISCLATIVSTLVEKKRRDFAIREGFVDNPKGVVNMSAMWLVPQCCLY 2042

Query: 1441 GLAEAFNAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLG 1500
            GL++  N  GQI+F+YSQ P++M+S+AV+L +LG G G+L+  +IV V+K  T K G   
Sbjct: 2043 GLSQGLNFTGQIEFYYSQFPKTMSSVAVSLCTLGFGVGNLVGCLIVKVVKDGTEKGGKDS 2102

Query: 1501 WLPNNLNKGHYDYYYWVLSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDE 1538
            WL +N+N+GHYDYYY +L ++ +VN L +   S  YG  ++     + WDE
Sbjct: 2103 WLASNINRGHYDYYYGLLFVLNLVNLLCFCTWSRAYGSTQD----IKYWDE 2131

BLAST of Lag0034450 vs. ExPASy TrEMBL
Match: A0A6J1F029 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441113 PE=4 SV=1)

HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 814/945 (86.14%), Postives = 871/945 (92.17%), Query Frame = 0

Query: 1   MDDSGNNSSEIGERHH-TPPKDFVCPITCNIFYDPVTLETGQTYERSAIQEWLDRGNSTC 60
           M++SGNN  E GE HH T PKDFVCPITCNIFYDPVT+ETGQTYERSAIQEWLDRGNSTC
Sbjct: 1   MEESGNNGLESGEMHHNTHPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNSTC 60

Query: 61  PITGQKLQNTQLPKTNYVLKRLIASWLEENPSFVSDKALDEANPVAVLTSPVSVISQASI 120
           PITGQKLQNTQLPKTNYVLKRLIASWL+ENP+FV+DKALDEA+  AVL SPVSVISQASI
Sbjct: 61  PITGQKLQNTQLPKTNYVLKRLIASWLKENPNFVTDKALDEADSAAVLISPVSVISQASI 120

Query: 121 NRSTNEVRHAITNLYSSEVLEEAESAVLCVERFWLEENVEMDIQHMLLKPPVINGLVEIL 180
           NRS  EVRHAI+N+ SSEVLEEAESAVLCVERFWLEENV+++IQHMLLKPPVINGLVEIL
Sbjct: 121 NRSM-EVRHAISNIVSSEVLEEAESAVLCVERFWLEENVDVEIQHMLLKPPVINGLVEIL 180

Query: 181 VNSVNLQVLRATVFLLSELGFKDTVVIQTLTRVESDVDCIVTLFKSGLMEAVVLIYQLGL 240
           +NSV+LQVLRA++FLLSELGFKD  VIQTLTRVESDVDCIVTLFKSGLMEAVVL+Y+ GL
Sbjct: 181 INSVDLQVLRASIFLLSELGFKDAAVIQTLTRVESDVDCIVTLFKSGLMEAVVLMYRFGL 240

Query: 241 SIESLQEMDMASSLLNVVKKKEGDVNKMRMSQKSAAVVLLRKILGRSKEGSLIAVAVLAE 300
           SI+ LQEMD+A SLLNVVKKKE DVNKM+++ KSAAV+LLRKILGRSKEGSLIAVAVLAE
Sbjct: 241 SIQRLQEMDLAGSLLNVVKKKE-DVNKMQLNPKSAAVILLRKILGRSKEGSLIAVAVLAE 300

Query: 301 NATESILDSLKAKQVEERIAAVGILLRCIQEDGKCRNIIADKADLTLILESFMEVSNEEQ 360
           NA ESIL SLKAKQVEERIAAVGILLRCIQEDGKCRN+IADKADL  IL SFMEVSN+EQ
Sbjct: 301 NAIESILVSLKAKQVEERIAAVGILLRCIQEDGKCRNMIADKADLAPILGSFMEVSNDEQ 360

Query: 361 FEIILFLSELVKLNRRTFNEQILQNIKNGGECSTMHSLLIYLQTALRDQSPVVAGLLLQL 420
           FEII+FLSELVKLNRRTFNEQILQNIK+GGECSTMHSLL+YLQTA RDQ PVVAG LLQL
Sbjct: 361 FEIIMFLSELVKLNRRTFNEQILQNIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLLQL 420

Query: 421 DILVEPRKMSIYREEAIDILISCLGDSDFPTAQISAAETIMSLQGRFSTSGRPLARYVLL 480
           DILVEPRK SIYREEA+D+L+SCLG+SDFPTAQISAAETIMSLQGRFSTSGRPL RYVLL
Sbjct: 421 DILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLTRYVLL 480

Query: 481 ERVGFMKGHMKLKRRDNGNSAPGEVELTIEEERAADEWERKMAFVLVSHDFGLLFEPLAK 540
           ERVGF KG MK KRRDN +S PG+VEL+I EERA DEWERKMAFVL SHDFGLLFEPLAK
Sbjct: 481 ERVGFAKGCMKPKRRDNNSSGPGDVELSIAEERATDEWERKMAFVLASHDFGLLFEPLAK 540

Query: 541 GLKSKYAALFSACFVSATWLSHMLRVLPDTGILEAARVCLLDHFMSIFATTTDIEEKALG 600
           GLKSKYAALFSACFVSATWLSHML+VLPDTGIL+ ARVCLLDHF+SIF TTTDIEEKAL 
Sbjct: 541 GLKSKYAALFSACFVSATWLSHMLKVLPDTGILDTARVCLLDHFVSIFITTTDIEEKALA 600

Query: 601 LIAMNSFIHEPEGQQSLSSNMKNIMRGLKELRRSTPLAFEMLKVLCEGEDSSTELWSHQV 660
           L+ MNSF+H+PEG Q LSSNMK+IMRGLKELRRST LAFEMLKVLC+GE+SS ELWSHQ 
Sbjct: 601 LLGMNSFVHQPEGLQYLSSNMKDIMRGLKELRRSTALAFEMLKVLCKGEESSAELWSHQE 660

Query: 661 LFQVDCSENGEVLSIAYFKDKIISGHSDGTIKVWSFRETIFHLLQEIQEHSKAVTSLAIM 720
           LF VDCS NGEVLSIAYFKDKIISGHSDGTIKVWS   T  H LQE QEHSK VTSLAI+
Sbjct: 661 LFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSLAII 720

Query: 721 ESEQKLYSGSLDKTIKVWSLGSDIIQCIQVHDVKDQIHNLVVSKTVACFIPHGAGIRVYS 780
           ESE+KLYSGSLDKTIKVWSLGSD+IQCIQVHDVKDQ+HNLVVSKT+ACFIPHGAGIRVYS
Sbjct: 721 ESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIRVYS 780

Query: 781 WGGESKLLNSSKHVKCLNLVHGKLYCGCQDSSIQAVDLATGTISYIHSGSRKLLGKANPV 840
           WGGESKLLNSSKHVK LNLV GKLYCGC DSSIQ VDLATGT+SYIH GSRKLLGK N V
Sbjct: 781 WGGESKLLNSSKHVKRLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKPNIV 840

Query: 841 QALQAYDEELFSASTSLDGVAVKIWSMSNYSAIGSLSTAMDVRTMTVSSDLIYLGGR-GV 900
           Q+LQ YDE+LFSAST+LDG AVKIWSMSN S I +LSTAMD+RTMTVSSDL YLGGR GV
Sbjct: 841 QSLQIYDEQLFSASTTLDGAAVKIWSMSNNSVIATLSTAMDIRTMTVSSDLTYLGGRGGV 900

Query: 901 VEIWGRDKHKKIDTLQTGRNCKVACMTLNEREEVLVIGTSDGWIQ 944
           VEIWGRDK  KIDTLQTGR CKVACMTLNERE+VLVIGTSDG IQ
Sbjct: 901 VEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLVIGTSDGRIQ 943

BLAST of Lag0034450 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 2.9e-163
Identity = 303/596 (50.84%), Postives = 408/596 (68.46%), Query Frame = 0

Query: 960  MEKYPSEKMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNE 1019
            ME  P+E    T  K     +    KGG+ TMPFIIANE FEK+++ GL  NMI YL  +
Sbjct: 1    MENPPNE----TEAKQIQTNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRD 60

Query: 1020 YNVDNAKGAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAIL 1079
            Y    AKG  VLFMWSA +NF P+ GAFLSDSYLGRF  ISI ++ + LGMV+LWLTA+L
Sbjct: 61   YRFGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAML 120

Query: 1080 PKARPPHC--KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDH 1139
            P+ +P  C   + G  C S+ A QL LLY +F L++IG+GGIRPCSLAFGADQL+   + 
Sbjct: 121  PQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENP 180

Query: 1140 KNQRTMQSFFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSP 1199
            KN+R ++SFF WYY S  V+V I+   +VY+Q   GW +G+GVP  LML + ++F + SP
Sbjct: 181  KNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASP 240

Query: 1200 LYVKLMANKSLFSSLAQVIVAAWKNRHLELPP--QTSDKWFYHKGSKLVTPTPKLRFLNK 1259
            LYV     KSLF+ LAQ IVAA+K R L LP    + D +++ K S++  P+ KLRFLNK
Sbjct: 241  LYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNK 300

Query: 1260 ACIIRNRETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAAL 1319
            AC+I NRE ++ S+G A  PWRL T  +VEELKA+I+V+PIWSTGI+++    Q +F  L
Sbjct: 301  ACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLL 360

Query: 1320 QAITMDRHITPH---FQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQ 1379
            QA +MDR ++ H   FQ PA SF +FTI+ L +WV +YD  +IPL +K   R    + K 
Sbjct: 361  QATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKL 420

Query: 1380 RMGIGLAISCLASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAF 1439
            RMG+GL +S LA A+SA +E  RR +AI +G AN    +V +SAMWLVPQY L GLAEA 
Sbjct: 421  RMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEAL 480

Query: 1440 NAIGQIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNL 1499
             AIGQ +FFY++ P+SM+SIA +LF LGM   SLLA+++++ + + T++NG   W+ +N+
Sbjct: 481  TAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNI 540

Query: 1500 NKGHYDYYYWVLSLMGVVNFLYYLICSWFYG---DEKEGMEASRVWDEKEAIEEEG 1546
            NKGHY+YYYWVL++M  +N +YY+ICSW YG   D+      + V +E+E I+  G
Sbjct: 541  NKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQVRNGRVNGVREEEELIDIVG 592

BLAST of Lag0034450 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 560.8 bits (1444), Expect = 3.3e-159
Identity = 296/585 (50.60%), Postives = 404/585 (69.06%), Query Frame = 0

Query: 972  SEKMETAQKVAIK---KGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYNVDNAKGA 1031
            +E  ET Q+   +   KGGL TMPFIIANE FEK+++ GL  NMI YL ++Y +   KG 
Sbjct: 8    TESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQ 67

Query: 1032 IVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPKARPPHC- 1091
             VLFMW A TNF+P+ GAFLSDSYLGRF  I I ++ +LLGMVVLWLTA+LP+ +P  C 
Sbjct: 68   TVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCV 127

Query: 1092 KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKNQRTMQSFF 1151
             + G  C SA + QL LLY +F L++IG+GGIRPCSLAFGADQL+   + KN+R ++SFF
Sbjct: 128  ATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFF 187

Query: 1152 NWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLYVKLMANKS 1211
             WYY S  V+V I+   +VY+Q+  GW +G+G+P  LML +  +F   SPLYVK   +KS
Sbjct: 188  GWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKS 247

Query: 1212 LFSSLAQVIVAAWKNRHLELPP--QTSDKWFYHKGSKLVTPTPKLRFLNKACIIRNRETD 1271
            LF+ LAQV+ AA+  R+L LP    + D ++  K S+L  P+ KLRFLNKAC I NR+ D
Sbjct: 248  LFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDED 307

Query: 1272 VDSNGMAKFPWRLSTIKRVEELKAVIRVLPIWSTGIVIAATIHQFTFAALQAITMDRHIT 1331
            + S+G+A   WRL T  +VE+LKA+++V+P+WSTGI+++  + Q +F  LQA +MDR ++
Sbjct: 308  LGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLS 367

Query: 1332 PH--FQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGLAISCL 1391
             +  FQ PA SF +FTI+ L  WV +YD  I+PL +K   R      K RMG+GL IS L
Sbjct: 368  SNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFL 427

Query: 1392 ASAVSAEIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQIQFFYS 1451
            A AVSA +E  RR  AI +G+AN     V +SAMWLVPQY L GLAEA   IGQ +FFY+
Sbjct: 428  AMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYT 487

Query: 1452 QLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHYDYYYWV 1511
            + P+SM+SIA +LF LGM   ++LA++I++ + K ++K GN+ W+ +N+NKGHYDYYYWV
Sbjct: 488  EFPKSMSSIAASLFGLGMAVANILASVILNAV-KNSSKQGNVSWIEDNINKGHYDYYYWV 547

Query: 1512 LSLMGVVNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEEEGTLN 1549
            L+++  VN +YY++CSW YG   + +   +V   ++  EE   LN
Sbjct: 548  LAILSFVNVIYYVVCSWSYGPTVDQVRNDKVNGMRKEEEEVIKLN 591

BLAST of Lag0034450 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 411.0 bits (1055), Expect = 4.3e-114
Identity = 234/547 (42.78%), Postives = 341/547 (62.34%), Query Frame = 0

Query: 985  KGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYNVDNAKGAIVLFMWSALTNFLPIG 1044
            KGGL TMPFI ANE+ EK++ VG HANMI YLT + ++   K A  L  ++  ++  P+ 
Sbjct: 24   KGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLL 83

Query: 1045 GAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPKARPPHCKSPGEFCVSADAGQLML 1104
            GAF++DS+ GRF  I+  +++  +GM +L ++AI+P  RPP CK   E CV AD  QL +
Sbjct: 84   GAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGE-EVCVVADTAQLSI 143

Query: 1105 LYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKNQRTMQSFFNWYYVSVGVSVSISVIF 1164
            LY + LL A+G+GGIRPC +AFGADQ ++   ++  +T  ++FNWYY  +G +V ++V  
Sbjct: 144  LYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTW-NYFNWYYFCMGAAVLLAVTV 203

Query: 1165 MVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLYVKLMANKSLFSSLAQVIVAAWKNRH 1224
            +V++Q+  GW +G G+P   M  S I F  G  LY  L+   S F+ L QV VAA++ R 
Sbjct: 204  LVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRK 263

Query: 1225 LELPPQTSDKWFYHK-------GSKLVTPTPKLRFLNKACIIRNRETDVDSNGMAKFPWR 1284
            L +    S  +F  +       G KL T T  + FL+KA I+   E D    G     WR
Sbjct: 264  LRMVSDPSLLYFNDEIDAPISLGGKL-THTKHMSFLDKAAIV--TEEDNLKPGQIPNHWR 323

Query: 1285 LSTIKRVEELKAVIRVLPIWSTGI-VIAATIHQFTFAALQAITMDRHITPHFQFPAASFA 1344
            LST+ RVEELK+VIR+ PI ++GI +I A   Q TF+  QA TM+RH+T  FQ PA S +
Sbjct: 324  LSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMS 383

Query: 1345 VFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGLAISCLASAVSAEIERKRR 1404
            VFT + +   +  YD + + +  KFT    G TF  RMGIG  IS +A+ V+  +E KR+
Sbjct: 384  VFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRK 443

Query: 1405 NRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQIQFFYSQLPRSMASIAVAL 1464
            + AI  G+ + P  IV +S +WL+PQY L G+AEAF +IG ++FFY Q P SM S A AL
Sbjct: 444  SVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATAL 503

Query: 1465 FSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLP-NNLNKGHYDYYYWVLSLMGVVNFLYY 1523
            F + +  G+ ++ ++V+++ K + K     WLP NNLN+G  +Y+YW+++++  VN +YY
Sbjct: 504  FWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYY 563

BLAST of Lag0034450 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 399.4 bits (1025), Expect = 1.3e-110
Identity = 223/577 (38.65%), Postives = 344/577 (59.62%), Query Frame = 0

Query: 978  AQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYNVDNAKGAIVLFMWSAL 1037
            A+KV  K GG R + FI+ NE  E++ ++GL AN + YLT  ++++    A V+ +WS  
Sbjct: 45   AEKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGF 104

Query: 1038 TNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPKARPPHCKSPGEF-CVS 1097
            TN  P+ GA++SD+Y+GRFK I+  +  TLLG++ + LTA  P+  P  C S     C  
Sbjct: 105  TNLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGG 164

Query: 1098 ADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKNQRTMQSFFNWYYVSVGV 1157
             +  Q+ +L      +++G+GGIRPCS+ FG DQ ++  + +  + + SFFNWYY++  V
Sbjct: 165  PNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTE-EGVKGVASFFNWYYMTFTV 224

Query: 1158 SVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLYVKLMANKSLFSSLAQVI 1217
             + I+   +VY+Q+   W++G+ +P GLM  + +MFF G   YV +    S+FS +AQVI
Sbjct: 225  VLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVI 284

Query: 1218 VAAWKNRHLELPPQTSDKWFYH----KGSKL--VTPTPKLRFLNKACIIRNRETDVDSNG 1277
            VAA K R L+LP +      Y+    K S L  +  + + R L+KA ++   E D+   G
Sbjct: 285  VAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEG 344

Query: 1278 MAKFPWRLSTIKRVEELKAVIRVLPIWSTGIV-IAATIHQFTFAALQAITMDRHITPHFQ 1337
                 WRL +++ VEE+K +IR++PIWS GI+ +AA   Q TF   QA+ MDR++ P F+
Sbjct: 345  PPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFE 404

Query: 1338 FPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGLAISCLASAVSA 1397
             PA S +V ++LT+ I++  YD + +P + + T   +G T  QR+G G+  +  +  V+ 
Sbjct: 405  IPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAG 464

Query: 1398 EIERKRRNRAILEGVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIGQIQFFYSQLPRSM 1457
             +ER RR R+I  G    P G+  MS  WL PQ  L GL EAFN IGQI+FF SQ P  M
Sbjct: 465  IVERMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHM 524

Query: 1458 ASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGHYDYYYWVLSLMGV 1517
             SIA +LFSL   G S L++ +V+V+ K +  +    WL  NLN G  DY+Y++++++GV
Sbjct: 525  RSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGV 584

Query: 1518 VNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEEEGT 1547
            VN +Y+  C+  Y   K G+      ++K + + E T
Sbjct: 585  VNLVYFWYCARGY-RYKVGLPIEDFEEDKSSDDVEMT 614

BLAST of Lag0034450 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 372.5 bits (955), Expect = 1.7e-102
Identity = 217/566 (38.34%), Postives = 333/566 (58.83%), Query Frame = 0

Query: 967  KMETTSEKMETAQKVAIKKGGLRTMPFIIANEVFEKISNVGLHANMIFYLTNEYNVDNAK 1026
            ++E T + +    +  I   G + MPFII NE FEK+  VG  +N++ YLT  +N+ +  
Sbjct: 2    EVEKTEKNIAEDDESKIIYRGWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSIT 61

Query: 1027 GAIVLFMWSALTNFLPIGGAFLSDSYLGRFKVISIGTVVTLLGMVVLWLTAILPKARPPH 1086
             A V+ ++   +NF  I  AFL DSY GR+K +S   +   LG V + LTA++    P  
Sbjct: 62   AAKVVNIYGGTSNFGTIVAAFLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQ 121

Query: 1087 C-KSPGEFCVSADAGQLMLLYFSFLLMAIGAGGIRPCSLAFGADQLEKPGDHKNQRTMQS 1146
            C K  G  C     GQ+M L  + +L+ IGAGGIRPC+L FGADQ + P   + +R ++S
Sbjct: 122  CAKEIGSVCNGPSIGQIMFLAGAMVLLVIGAGGIRPCNLPFGADQFD-PKTKEGKRGIES 181

Query: 1147 FFNWYYVSVGVSVSISVIFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFIGSPLYVKLMAN 1206
            FFNWY+ +   +  +S+  +VY+Q+   W +G  +P  LML   I+FF GS LYVK+ A+
Sbjct: 182  FFNWYFFTFTFAQMVSLTLIVYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKAS 241

Query: 1207 KSLFSSLAQVIVAAWKNRHLELPPQTSDKWFY----HKGSKLVTPTPKLRFLNKACIIRN 1266
             S   S+ +VIV A K R L+ P   ++ + Y     K SKL   T + RFL+K+  I+ 
Sbjct: 242  GSPIHSITRVIVVAIKKRRLK-PVGPNELYNYIASDFKNSKL-GHTEQFRFLDKSA-IQT 301

Query: 1267 RETDVDSNGMAKFPWRLSTIKRVEELKAVIRVLPIW-STGIVIAATIHQFTFAALQAITM 1326
            ++  ++ +G     W+L ++++VEE+K VIRVLP+W S  +   A I Q T+   Q++  
Sbjct: 302  QDDKLNKDGSPVDAWKLCSMQQVEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQS 361

Query: 1327 DRHITP-HFQFPAASFAVFTILTLTIWVAIYDLIIIPLLAKFTRRSNGFTFKQRMGIGLA 1386
            DR + P  FQ PA S+ VF +L +TI++ IYD +++P L K+T R  G T  QR+G GL 
Sbjct: 362  DRRLGPGSFQIPAGSYTVFLMLGMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLF 421

Query: 1387 ISCLASAVSAEIERKRRNRAILE---GVANVPGGIVKMSAMWLVPQYCLAGLAEAFNAIG 1446
            +   +  VSA +E+ RR  A+ +   G+A   G I  MS MWL+PQ  L G+A+A   +G
Sbjct: 422  LCITSMMVSAIVEQYRRKVALTKPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVG 481

Query: 1447 QIQFFYSQLPRSMASIAVALFSLGMGGGSLLAAIIVSVIKKETTKNGNLGWLPNNLNKGH 1506
            Q++F+Y Q P +M S A +L+  G+G  S L+  ++S +   T       WLP +LNKG 
Sbjct: 482  QMEFYYKQFPENMRSFAGSLYYCGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGR 541

Query: 1507 YDYYYWVLSLMGVVNFLYYLICSWFY 1523
             +Y+Y++++ M  +N  Y+L+ S +Y
Sbjct: 542  LEYFYFLVAGMMTLNLAYFLLVSHWY 563