Lag0034425 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034425
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr3: 7263899 .. 7265336 (+)
RNA-Seq ExpressionLag0034425
SyntenyLag0034425
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCGGGGGACAGGGGAAGCGGGTCCTATGTGTGGAAGAGTCTGCTGTGGGGAAGAGATTTGCTACAACAAGGGTTGAGGTGGCGTGTTGGAAATGGGAAGTCGATTCGCATTATGGAAGATAGTTGGATTCCTAGAGAATCTACTCTAAAAGTGATCCATAAAACAGGTATCAATCCTGATGCTAAGGTGGAGAGCTTGCTCAATTTGGATAATTCTTGGAATGTGAACCTGATCCAAGAATTATTCAAGGAGGAGGATGCTGAGGCTATTGTTAGTATACCCAGACCAAGGAGGAGAGTGGAGGACAAGCTTATATGGCATTTTGAGAGAAATGGCATGTACTCAGCTCGAAGTGGATACAAACTGGCATGCTCTCTAAGAGGAAATGTGGGTAATTCTGACCAAAGGAGAATGAAACAATGGTGGAACTTTCTGTGGACTAGGAAAATTCCTAACAAGATTAAATTCTTTTGTTGGAGGCTGTTTTTCAATTACCTCCCTACCCTGTCGAATCTTAAAAAACGTGGTAGGGATGTTTCAGGTGAATGCCCCAGATGTAGGAGGAAGGAGAAGACTGGTTTCCATGCTGTTTGGGAATGCAGACACATCAAACAATGCTGGCGGGAGTCGCCTTTCTACCCAAGACCGGGGCCTCCTTCTATCCAGCATGCTGCCGACTTATTGTGGTGGTGTTGGGAGAATCTGGCAGAGGACCGGTTTGATGAGTTTGTGGCTTTAAGCTGGTGGGCGTGGAGCCGACGAAATAAGGTAGTAGTTGGAGGGGGGTGTGTGTCTGGGATTGAGGATGGAGGTTGGAACTGGATCTCTGAATATCTATGTCAATTCAAGGATTTTCAGGGACGGAAGTGTGGGGCGGAGGGGGGGGGTCCGGAGGATTGATGCTCAGTGGGTGGCTCCGATTTCTCCGTCATTCAAGCTGAATACCGATGTGGGGACAAGCAGGAAAACTCAAACAAGTAGTATTGGTGCGATAGTGAGAAATGATAAAGGAGAAGTGATGCTTACTATGATGAAATTGATCCCGTTTGTGCACGATGTTGACACTTTGGAGGCAATGGCGATCCGTGAATCCTTGGTGGTAGCAATGGAAGTCGGATTATTGGCACTGGAAGTGGAATCGGACTCAGCCCGAGTCGTCGCGTTGCTTAGATCGGAGATGGAGGATGTCTCGGAAGTCGGCGAGGTGGTGAAGGAGATCAAGCTCCTGGCGAGAGGACTGCCCGCATGTTCATTTGGCTGGTGCCGGAGGAGCGCCAATTCGGCGGCGCATGCGGTGGCGAGATGGGCTCTCGAGCAGAAGGTTGAGTGGCGTTGTCCTGTACACATTTGCTCTAAAAAAAAAAACAAAAAAGAAAAAAAAGAAAAAAAACTTTTATCTATTATTTATCCCACATTTTAGTGGACACTCTGA

mRNA sequence

ATGGAGGCGGGGGACAGGGGAAGCGGGTCCTATGTGTGGAAGAGTCTGCTGTGGGGAAGAGATTTGCTACAACAAGGGTTGAGGTGGCGTGTTGGAAATGGGAAGTCGATTCGCATTATGGAAGATAGTTGGATTCCTAGAGAATCTACTCTAAAAGTGATCCATAAAACAGGTATCAATCCTGATGCTAAGGTGGAGAGCTTGCTCAATTTGGATAATTCTTGGAATGTGAACCTGATCCAAGAATTATTCAAGGAGGAGGATGCTGAGGCTATTGTTAGTATACCCAGACCAAGGAGGAGAGTGGAGGACAAGCTTATATGGCATTTTGAGAGAAATGGCATGTACTCAGCTCGAAGTGGATACAAACTGGCATGCTCTCTAAGAGGAAATGTGGGTAATTCTGACCAAAGGAGAATGAAACAATGGTGGAACTTTCTGTGGACTAGGAAAATTCCTAACAAGATTAAATTCTTTTGTTGGAGGCTGTTTTTCAATTACCTCCCTACCCTGTCGAATCTTAAAAAACGTGGTAGGGATGTTTCAGGTGAATGCCCCAGATGTAGGAGGAAGGAGAAGACTGGTTTCCATGCTGTTTGGGAATGCAGACACATCAAACAATGCTGGCGGGAGTCGCCTTTCTACCCAAGACCGGGGCCTCCTTCTATCCAGCATGCTGCCGACTTATTGTGGTGGTGTTGGGAGAATCTGGCAGAGGACCGGTTTGATGAGTTTGTGGCTTTAAGCTGGTGGGCGTGGAGCCGACGAAATAAGGTAGTAGTTGGAGGGGGGGACGGAAGTGTGGGGCGGAGGGGGGGGGTCCGGAGGATTGATGCTCAGTGGGTGGCTCCGATTTCTCCGTCATTCAAGCTGAATACCGATGTGGGGACAAGCAGGAAAACTCAAACAAGTAGTATTGGTGCGATAGTGAGAAATGATAAAGGAGAAGTGATGCTTACTATGATGAAATTGATCCCGTTTGTGCACGATGTTGACACTTTGGAGGCAATGGCGATCCGTGAATCCTTGGTGGTAGCAATGGAAGTCGGATTATTGGCACTGGAAGTGGAATCGGACTCAGCCCGAGTCGTCGCGTTGCTTAGATCGGAGATGGAGGATGTCTCGGAAGTCGGCGAGGTGGTGAAGGAGATCAAGCTCCTGGCGAGAGGACTGCCCGCATGTTCATTTGGCTGGTGCCGGAGGAGCGCCAATTCGGCGGCGCATGCGGTGGCGAGATGGGCTCTCGAGCAGAAGTGGACACTCTGA

Coding sequence (CDS)

ATGGAGGCGGGGGACAGGGGAAGCGGGTCCTATGTGTGGAAGAGTCTGCTGTGGGGAAGAGATTTGCTACAACAAGGGTTGAGGTGGCGTGTTGGAAATGGGAAGTCGATTCGCATTATGGAAGATAGTTGGATTCCTAGAGAATCTACTCTAAAAGTGATCCATAAAACAGGTATCAATCCTGATGCTAAGGTGGAGAGCTTGCTCAATTTGGATAATTCTTGGAATGTGAACCTGATCCAAGAATTATTCAAGGAGGAGGATGCTGAGGCTATTGTTAGTATACCCAGACCAAGGAGGAGAGTGGAGGACAAGCTTATATGGCATTTTGAGAGAAATGGCATGTACTCAGCTCGAAGTGGATACAAACTGGCATGCTCTCTAAGAGGAAATGTGGGTAATTCTGACCAAAGGAGAATGAAACAATGGTGGAACTTTCTGTGGACTAGGAAAATTCCTAACAAGATTAAATTCTTTTGTTGGAGGCTGTTTTTCAATTACCTCCCTACCCTGTCGAATCTTAAAAAACGTGGTAGGGATGTTTCAGGTGAATGCCCCAGATGTAGGAGGAAGGAGAAGACTGGTTTCCATGCTGTTTGGGAATGCAGACACATCAAACAATGCTGGCGGGAGTCGCCTTTCTACCCAAGACCGGGGCCTCCTTCTATCCAGCATGCTGCCGACTTATTGTGGTGGTGTTGGGAGAATCTGGCAGAGGACCGGTTTGATGAGTTTGTGGCTTTAAGCTGGTGGGCGTGGAGCCGACGAAATAAGGTAGTAGTTGGAGGGGGGGACGGAAGTGTGGGGCGGAGGGGGGGGGTCCGGAGGATTGATGCTCAGTGGGTGGCTCCGATTTCTCCGTCATTCAAGCTGAATACCGATGTGGGGACAAGCAGGAAAACTCAAACAAGTAGTATTGGTGCGATAGTGAGAAATGATAAAGGAGAAGTGATGCTTACTATGATGAAATTGATCCCGTTTGTGCACGATGTTGACACTTTGGAGGCAATGGCGATCCGTGAATCCTTGGTGGTAGCAATGGAAGTCGGATTATTGGCACTGGAAGTGGAATCGGACTCAGCCCGAGTCGTCGCGTTGCTTAGATCGGAGATGGAGGATGTCTCGGAAGTCGGCGAGGTGGTGAAGGAGATCAAGCTCCTGGCGAGAGGACTGCCCGCATGTTCATTTGGCTGGTGCCGGAGGAGCGCCAATTCGGCGGCGCATGCGGTGGCGAGATGGGCTCTCGAGCAGAAGTGGACACTCTGA

Protein sequence

MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGINPDAKVESLLNLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARSGYKLACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWENLAEDRFDEFVALSWWAWSRRNKVVVGGGDGSVGRRGGVRRIDAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCRRSANSAAHAVARWALEQKWTL
Homology
BLAST of Lag0034425 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 247.7 bits (631), Expect = 1.8e-61
Identity = 146/444 (32.88%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 1    MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
            MEA   G+ SY+W+S+LWGRDLL++GLRWR+GNG S+ I  D+W+P + TLK++    + 
Sbjct: 689  MEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLP 748

Query: 61   PDAKVESLL-NLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSAR 120
              ++V SL+ + +  W  +++++ F  ++A+ I+SIP  R   ED+LIW++E+ G+YS R
Sbjct: 749  LVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVR 808

Query: 121  SGYKLACSLRGNV---GNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKK 180
            SGYK+A      V    +S    ++ WWN  W   IPNKIK F WRL  + LPT  NL K
Sbjct: 809  SGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSK 868

Query: 181  RGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWEN 240
            RG +++  C  C R  +   H  W C+  +  W  S F  +  P  I   +       E+
Sbjct: 869  RGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKF-GKLSPFLILRESH------ES 928

Query: 241  LAEDRFDEFVALSWWAWSRRNKVVVGGGDGSVGRRG------------------------ 300
            L++  F+E   + W  W++RN         +V + G                        
Sbjct: 929  LSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITG 988

Query: 301  -GVRRIDAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDV 360
                  +  W  P    +K+NTD       Q + +G I+ ND+G+VM    K +  +  V
Sbjct: 989  RVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSV 1048

Query: 361  DTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGL 416
            D  EA+A  E L +A E+G                +   +ED+SE GE+V + K      
Sbjct: 1049 DMAEAIAAVEGLQLASEIG----------------MHPALEDLSETGEIVLKAKNFWTQS 1108

BLAST of Lag0034425 vs. NCBI nr
Match: XP_023904177.1 (uncharacterized protein LOC112015942 [Quercus suber])

HSP 1 Score: 245.4 bits (625), Expect = 9.0e-61
Identity = 149/447 (33.33%), Postives = 216/447 (48.32%), Query Frame = 0

Query: 1   MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
           ++A +  + S+VW+S++    +L+ G  WRVG+G SI+I+ D WIP+  T KV+   G++
Sbjct: 44  LDAVESPNCSFVWRSIMAAIPILKAGCCWRVGDGTSIKILTDKWIPKHPTNKVLFSAGVD 103

Query: 61  -PDAKVESLLNLD-NSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSA 120
             +  V  L+  D + W  +LI   F  EDA+AI  IP  RRRV+D +IW   +NG YS 
Sbjct: 104 VGEWLVADLIEPDSHCWKHDLIMNTFHREDAKAICRIPLSRRRVDDSIIWLHNKNGRYSV 163

Query: 121 RSGYKLACSL-------RGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTL 180
           +SGY +A  +         + GN +Q    Q W  LW   +PNKIK F WR     LPT 
Sbjct: 164 KSGYYVARQVLRSENWAECSKGNDEQ----QVWKALWKMNVPNKIKIFGWRACQGILPTR 223

Query: 181 SNLKKRGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLW 240
            NL KR       CP C +  +   H +WEC   +  W  S    +  P        L  
Sbjct: 224 LNLAKRKIIAETFCPICLQSPENEMHVIWECPVAQDVWAGSRIKLQKCPLGQPDMVQLFL 283

Query: 241 WCWENLAEDRFDEFVALSWWAWSRRNKVVVGGGDGSVGRRGGVRRIDAQWV--------- 300
           +    L  +    F+  +W  WS+RN V+ G   G +   G + + D +W+         
Sbjct: 284 YLLHRLEPEDMALFLVQAWMLWSQRNSVLHG---GKLKEPGWLNKRDVEWLEEFAQVQDQ 343

Query: 301 ---------------APISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPF 360
                           P  P FK+N D      +  S  GA++RN+ GEVM  +  + P 
Sbjct: 344 LDVLQPRATVGNVWRPPAHPFFKMNFDAAIFNDSGRSGFGAVIRNELGEVMAALSAIGPS 403

Query: 361 VHDVDTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLL 415
           V   D  E +A R+++  A++ G   L +E DS  V+  L S   D+S VG VV +IK L
Sbjct: 404 VSSSDEAETLACRKAMEFAVDAGFNELIIEGDSINVIKALSSSSPDLSVVGNVVADIKCL 463

BLAST of Lag0034425 vs. NCBI nr
Match: XP_030931246.1 (uncharacterized protein LOC115957168 [Quercus lobata])

HSP 1 Score: 240.7 bits (613), Expect = 2.2e-59
Identity = 144/440 (32.73%), Postives = 221/440 (50.23%), Query Frame = 0

Query: 1    MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHK-TGI 60
            +EA D  + SYVWKS+L  +++L+ G  WRVG G  IR+M + WIP   +  VIH+   +
Sbjct: 853  LEAKDASNSSYVWKSILAAQEILKNGCCWRVGTGVDIRVMAEKWIPNHPSNMVIHQPQEV 912

Query: 61   NPDAKVESLLNLD-NSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSA 120
              + +V  L++ +  +WN NLI+  F +EDA+AI+ IP  RR V D + W F R+  YS 
Sbjct: 913  EWEWRVVELIDWNVGAWNKNLIEMKFHKEDADAILRIPLSRRFVVDSIFWLFNRDEDYSV 972

Query: 121  RSGYKLACSLRGNVGNSDQRRM----KQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNL 180
            +SGYK+AC +R       +  M    +  W  +W   IPNKIK F WR     LPT +NL
Sbjct: 973  KSGYKVACKVRKEASMQGESSMAGDSQLVWRKIWQMHIPNKIKVFVWRACHGILPTRANL 1032

Query: 181  KKRGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCW 240
             ++       C  C+   +T  H +WEC   +  W  S    + G   +Q   +L+    
Sbjct: 1033 LQKHVVEDSTCVLCKCTSETELHVLWECGVAQDIWAGSMRKLQKGHDFLQLVEELML--- 1092

Query: 241  ENLAEDRFDEFVALSWWAWSRRNKVVVGG---GDGSVGRRGG-----------------V 300
              L+ +  + F   +W  W++RN V  GG       + +R G                 V
Sbjct: 1093 -KLSREELELFFVQAWLIWTQRNTVTFGGVIQDPSRLVKRAGEYLEEFKMSQVQLAMSTV 1152

Query: 301  RRIDAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTL 360
                ++W+ P   S+KLN D    +  + S  G ++RN+ GEVM       P + D +  
Sbjct: 1153 SARSSRWIPPPGLSYKLNFDAAIFQDGEASGFGVVIRNNGGEVMAAFSARGPAISDSEEA 1212

Query: 361  EAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPAC 415
            E +A R++L  A + G   L +E D++ V+ ++ S     S +G +  ++  LA GLP  
Sbjct: 1213 EVLACRKALEFAADSGFTELILEGDNSVVMNVISSSRMFQSRLGHLYSDVNCLATGLPVL 1272

BLAST of Lag0034425 vs. NCBI nr
Match: XP_024956542.1 (uncharacterized protein LOC112498908 [Citrus sinensis])

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-58
Identity = 143/433 (33.03%), Postives = 216/433 (49.88%), Query Frame = 0

Query: 10   SYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGINPDAKVESLL 69
            SY+W+S+LWGR ++  G RWR+GNG+ + I + +WIP+  T K + K  +  +A V  L+
Sbjct: 675  SYIWRSILWGRQIICGGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPTMPSEALVSELI 734

Query: 70   NLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARSGYKLACSLR 129
            N +N W+  LI + F + DA+ I  IP PRR  ED+LIWHF ++G Y+ +SGY+ A  +R
Sbjct: 735  NGENCWDEELIYKHFDKMDADVITQIPLPRRLREDELIWHFGKSGQYTVKSGYQRALKIR 794

Query: 130  GNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRDVSGECPRCR 189
                 S     K  WN +W+  +P KI+ F WR   N LP+  NL KR       C  C+
Sbjct: 795  FPAMPSSSESSKNEWNIIWSLALPKKIRIFVWRAAKNLLPSAENLWKRKIVQEPTCQLCK 854

Query: 190  RKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWENLAEDRFDEFVALS 249
               +  FHA+ +C+  K+ WR S FY        Q    LL       +    D F  + 
Sbjct: 855  MGIENVFHALVDCKAAKKVWRLSLFYIDIQAAPGQDILSLLHGVKRMRSNADVDLFAVML 914

Query: 250  WWAWSRRN-------------------------KVVVGGGDGSVGRRGGVRRIDAQWVAP 309
            W  W+ RN                         K V    D S G++  V ++   W  P
Sbjct: 915  WAKWNARNQWLFKGKRENPQSVVAKAEAVMEAYKRVQPSADVSHGKQQKVAQLG--WNPP 974

Query: 310  ISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIRESLV 369
                 K+NTD  T+ +   + +GA++R++ G+V  T +K+  F   V   EA A+   L 
Sbjct: 975  QEGFVKINTDAATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQ 1034

Query: 370  VAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCRRSAN 418
            VA +  +  + +ESDS  VV+L+ +     SE+  VV EI+ L       S  +  RS N
Sbjct: 1035 VAKDAHVKDVIMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCN 1094

BLAST of Lag0034425 vs. NCBI nr
Match: XP_030964861.1 (uncharacterized protein LOC115986145 [Quercus lobata])

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-58
Identity = 149/443 (33.63%), Postives = 219/443 (49.44%), Query Frame = 0

Query: 1    MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIH-KTGI 60
            M+A D  + SYVWKS++  + +L +G  WRVG+G  I+++ D WIP   T  VIH  + +
Sbjct: 711  MDASDSPNSSYVWKSIMAAKPILDRGCYWRVGDGADIKVLSDKWIPNYPTNGVIHPPSEV 770

Query: 61   NPDAKVESLLNLD-NSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSA 120
              D  V  L++ D   W  + I   F +EDA+AI+ I        D ++W   + G+YS 
Sbjct: 771  GRDWYVSDLIDPDLKWWRRDFIGTNFHKEDADAILRISLSHSHTSDAIVWSQNKRGVYSV 830

Query: 121  RSGYKLACSLRGNVGN------SDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLS 180
            +SGY +A   R N+ N      S   + +Q W+ LW  ++PNKIK F WR   N LPT  
Sbjct: 831  KSGYYVA---RQNLKNENWAECSSSPKGRQVWSKLWKLRVPNKIKVFGWRACHNILPTRD 890

Query: 181  NLKKRGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWW 240
            NL +R       C  C R  +TG HA+WEC   K  W  +    +      Q    L   
Sbjct: 891  NLVRRKILEDDGCMLCSRMAETGVHALWECAVAKDVWSSNMIRLQKCGQDQQDVLQLFQE 950

Query: 241  CWENLAEDRFDEFVALSWWAWSRRNKVVVGG---GDGSVGRRG-----------GVRRID 300
              E L+  +F+ F+  SW  W++RN V+ GG       + +R            G  +I 
Sbjct: 951  LMERLSTAKFEAFLVQSWLIWNQRNTVMHGGKLKDPSWLNKRAKDYLEDFHQAQGQLQIP 1010

Query: 301  A-------QWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDV 360
                     W  P  P +KLN D     K   S +GA++RN   EVM  M    P V D 
Sbjct: 1011 VTSSSGHDTWFPPPEPIYKLNFDAAIFSKLGCSGVGAVIRNVHDEVMAAMSARGPPVIDN 1070

Query: 361  DTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGL 415
               E +A R++L  A++ G   L +E D+  V+  + +   D+S +G ++++IK LA GL
Sbjct: 1071 SEAEILACRKALEFAVDAGFTDLVIEGDNENVMRSVSASGADLSRLGHIIQDIKWLAHGL 1130

BLAST of Lag0034425 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 5.6e-13
Identity = 91/415 (21.93%), Postives = 157/415 (37.83%), Query Frame = 0

Query: 6   RGSGSYVWKSLLWG-RDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGINP--- 65
           +GS S  W+S+  G RD++  G+ W  G+G+ IR   D W+  +  L++    G  P   
Sbjct: 151 KGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKPLLEL--DNGERPTDC 210

Query: 66  -DAKVESLLNLDNSWNVNLIQELFKEEDAEAIVSIPRPR-RRVEDKLIWHFERNGMYSAR 125
                + L      W+   I           + ++         D+L W F ++G +S R
Sbjct: 211 DTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVTGARDRLSWKFSQDGQFSVR 270

Query: 126 SGYKLACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGR 185
           S Y++       V    +  M  ++N LW  ++P ++K F W +    + T     +R  
Sbjct: 271 SAYEML-----TVDEVPRPNMASFFNCLWKVRVPERVKTFLWLVGNQAVMTEEERHRRHL 330

Query: 186 DVSGECPRCRRKEKTGFHAVWECRHIKQCW-RESPFYPRPGPPSIQHAADLLWWCWENLA 245
             S  C  C+   ++  H + +C      W R  P   + G      +  L  W ++NL 
Sbjct: 331 SASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRRQQG----FFSKSLFEWLYDNLG 390

Query: 246 EDRFDE-------FVALSWWAWSRRNKVVVG-----------------------GGDGSV 305
           +    E       F  + WW W  R   + G                        G+  V
Sbjct: 391 DRSGCEDIPWSTIFAVIIWWGWKWRCGNIFGENTKCRDRVKFVKEWAVEVYRAHSGNVLV 450

Query: 306 G-RRGGVRRIDAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPF 365
           G  +  V R+   WV+P     K+NTD  +      +S G ++R+  G        L   
Sbjct: 451 GITQPRVERM-IGWVSPCVGWVKVNTDGASRGNPGLASAGGVLRDCTG-AWCGGFSLNIG 510

Query: 366 VHDVDTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVK 383
                  E   +   L  A E  +  +E+E DS  +V  L++ + D   +  +V+
Sbjct: 511 RCSAPQAELWGVYYGLYFAWEKKVPRVELEVDSEVIVGFLKTGISDSHPLSFLVR 552

BLAST of Lag0034425 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 8.8e-62
Identity = 146/444 (32.88%), Postives = 222/444 (50.00%), Query Frame = 0

Query: 1    MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
            MEA   G+ SY+W+S+LWGRDLL++GLRWR+GNG S+ I  D+W+P + TLK++    + 
Sbjct: 689  MEAKISGNPSYIWRSILWGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLP 748

Query: 61   PDAKVESLL-NLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSAR 120
              ++V SL+ + +  W  +++++ F  ++A+ I+SIP  R   ED+LIW++E+ G+YS R
Sbjct: 749  LVSRVSSLVDHEEGGWQGDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVR 808

Query: 121  SGYKLACSLRGNV---GNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKK 180
            SGYK+A      V    +S    ++ WWN  W   IPNKIK F WRL  + LPT  NL K
Sbjct: 809  SGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSK 868

Query: 181  RGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWEN 240
            RG +++  C  C R  +   H  W C+  +  W  S F  +  P  I   +       E+
Sbjct: 869  RGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKF-GKLSPFLILRESH------ES 928

Query: 241  LAEDRFDEFVALSWWAWSRRNKVVVGGGDGSVGRRG------------------------ 300
            L++  F+E   + W  W++RN         +V + G                        
Sbjct: 929  LSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITG 988

Query: 301  -GVRRIDAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDV 360
                  +  W  P    +K+NTD       Q + +G I+ ND+G+VM    K +  +  V
Sbjct: 989  RVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSV 1048

Query: 361  DTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGL 416
            D  EA+A  E L +A E+G                +   +ED+SE GE+V + K      
Sbjct: 1049 DMAEAIAAVEGLQLASEIG----------------MHPALEDLSETGEIVLKAKNFWTQS 1108

BLAST of Lag0034425 vs. ExPASy TrEMBL
Match: A0A803PM68 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 7.5e-61
Identity = 140/439 (31.89%), Postives = 218/439 (49.66%), Query Frame = 0

Query: 1   MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
           +EA      S+VW+SL+WG+ ++Q+G RWR+GNG S+R++ED W+PR  T KV  K  + 
Sbjct: 236 LEAKSGNHASFVWRSLVWGKKIIQKGYRWRIGNGNSVRVLEDPWLPRPVTFKVYDKPSLP 295

Query: 61  PDAKVESLLNLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARS 120
               V  L+  +  W+   I+  F   DAE I+ +P     +EDK++WH+ +NG YS RS
Sbjct: 296 EGMYVVDLMKGNGEWDEEFIRAFFNPTDAELILGMPTSEWEIEDKILWHYSKNGEYSVRS 355

Query: 121 GYKLACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRD 180
           GY++A +L+ +   S+    ++WW  LW  KIP K+K F W++  ++LPT S L  R   
Sbjct: 356 GYRMAAALQVHDIQSNTEATERWWRLLWKLKIPPKVKHFVWKMAHSWLPTNSALAYRKVQ 415

Query: 181 VSGECPRCRR-KEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWENLAE 240
           V   C RC     +  FHA+W CR   + W+ + F+ R      +     L       ++
Sbjct: 416 VEPYCTRCSSGAYENVFHALWGCRVNCEVWKLTGFHGRIKRQGKEDVLAFLMRLSGMFSK 475

Query: 241 DRFDEFVALSWWAWSRRNKVVVGG-------------------GDGSVGRRGGVRRIDAQ 300
           D ++ F+ LSW  W  RN V  GG                    DG   ++ GV R +A+
Sbjct: 476 DDWEFFLILSWNLWYIRNSVNHGGHKPQAAAVVDWCGRFLSEFKDGCGPKKHGVARAEAR 535

Query: 301 WVAPISPSFKLNTDVGTS-RKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAI 360
           W  P+  SFK+N D G        +S  ++VR+  G V      ++  V+     E +AI
Sbjct: 536 WQPPMRDSFKVNVDAGVKVGGGGYTSFSSVVRDHTGRVCAANATVVRKVYSPLHAELVAI 595

Query: 361 RESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWC 419
              L   +   L +  VE+D  + V L++ E E   ++  ++  IK L + +      + 
Sbjct: 596 SAGLQAGIHTRLPSFIVETDCLQAVNLVQQEEEGCRDLDGLIAHIKCLLQDVRVGGINFV 655

BLAST of Lag0034425 vs. ExPASy TrEMBL
Match: A0A2N9F2A9 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8891 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 9.7e-61
Identity = 145/423 (34.28%), Postives = 215/423 (50.83%), Query Frame = 0

Query: 1   MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
           +EA   G GSY W+S+L  RDL+ +G  WRVG+G  +RI  D W+P      +I     +
Sbjct: 279 LEANANGKGSYAWRSILSARDLISKGSVWRVGSGAGVRIWGDRWLPGSQHHCIISPPPTH 338

Query: 61  PD-AKVESLLNLD-NSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSA 120
              + V  L++ D   W   L+ ELF   +A AI+ IP   R + D L+W   +NG+YS 
Sbjct: 339 TSISHVSHLIDPDLRIWKAGLVNELFLPHEATAILGIPLSHRSLTDCLVWGATKNGVYSV 398

Query: 121 RSGYK--LACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKK 180
           RSGYK  L  S R + G+SD  RM Q W  +W+  +P KI+ F WR   N LPT SNL  
Sbjct: 399 RSGYKFLLNESHREDPGSSDPTRMSQLWKSVWSLNVPPKIRHFLWRACHNSLPTKSNLHH 458

Query: 181 RGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWEN 240
           R       C  C  + ++  HA+W+C+ ++  W+   +  + G  +     DLL  C+  
Sbjct: 459 RHILDDPSCTNCSYQSESTIHALWQCKMVQPVWQSISWGSKLGASNFMGFIDLLHQCFSI 518

Query: 241 LAEDRFDEFVALSWWAWSRRNKV-VVGGGDGSVGRRGGVRRIDAQWVAPISPSFKLNTDV 300
           L+      F  +SW  W RRN++ +    D +    G V  +  +W  P   S+K+N D 
Sbjct: 519 LSPSEVQLFSMISWSIWYRRNRLRLQQPADNNFQVPGSVELV--KWNPPAQGSYKVNYDG 578

Query: 301 GTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIRESLVVAMEVGLLALE 360
                   + IG IVRN +GEVM ++   IPF H V+ LEA A R ++  A ++G     
Sbjct: 579 AVFSDFNAAGIGVIVRNHQGEVMGSLSHRIPFPHSVEALEASAARSAIQFAKDLGFTKFV 638

Query: 361 VESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCRRSANSAAHAVARWAL 419
           +E DS  VV  L       +  G ++ +IK +A+ L +  F   +R  N  AH +A+ A 
Sbjct: 639 LEGDSKTVVDALLLREPCTTIYGHIIDDIKQIAQSLQSVLFLHTKREGNVMAHLLAKRAR 698

BLAST of Lag0034425 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.4e-59
Identity = 140/438 (31.96%), Postives = 219/438 (50.00%), Query Frame = 0

Query: 1   MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGIN 60
           +EAG   + S+VW+SL+WG+ L+ +G RWRVGNG+S+R++ED W+PR  T KV  +  + 
Sbjct: 243 LEAGCGANASFVWRSLVWGKKLILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLP 302

Query: 61  PDAKVESLLNLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARS 120
            +  V  L   D  W+   I+ +F   D + I+ IP      EDK++WH+ + G YS +S
Sbjct: 303 ANLYVTDLKLADGQWDEGFIRSIFNPTDVDLILGIPCSDWDFEDKILWHYSKYGEYSVKS 362

Query: 121 GYKLACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRD 180
           GY++A S       S++  + QWW  LW  KIP K+K F W++  N+LP   NL KRG  
Sbjct: 363 GYRMAASFTTEQHQSNEHSIVQWWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKRGIA 422

Query: 181 VSGECPRC-RRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWENLAE 240
            S  C RC    +++  HA+WEC+  K  WR S  Y        +    +L        +
Sbjct: 423 SSVVCSRCSSHVDESVAHALWECKASKGYWRVSGLYDDLKQMLGEDNLTMLMRIAAEWDK 482

Query: 241 DRFDEFVALSWWAWSRRNKVVVGG------------GD------GSVGRRGGVRRI-DAQ 300
           ++ + F+ +SW  W+ RN VV GG            G+      G  GR    R   D++
Sbjct: 483 EKLEFFLLVSWNIWNVRNTVVHGGYHPKPEEMIEWCGNFLADFRGDTGRERSQRSSEDSR 542

Query: 301 WVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIR 360
           WV P      +N D G  +    S +G +VR+  G V+     ++        LE MAI+
Sbjct: 543 WVPPARDQVTINVDAGVKQGGLISGLGYVVRDAAGVVLSAAATVLQQELPPLQLELMAIK 602

Query: 361 ESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCR 419
           + + V ++  L    VE+D  + V L++++     ++  ++  I+ L          +  
Sbjct: 603 KGIQVGIQQRLQRFSVETDCLQAVHLIQNKENGCRDIDGLLNHIRALISYDSFVGISFVF 662

BLAST of Lag0034425 vs. ExPASy TrEMBL
Match: A0A803QPB7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 5.3e-59
Identity = 138/443 (31.15%), Postives = 221/443 (49.89%), Query Frame = 0

Query: 2    EAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGINP 61
            EA     GS VW+ +LWGR+LL++G RW VGNG  IRI ED W+PR     +  K  I  
Sbjct: 1247 EAKQGHYGSTVWRGILWGRELLRKGTRWCVGNGSKIRINEDQWLPRSQPFTLRSKVQIPA 1306

Query: 62   DAKVESLLNLDNSWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARSG 121
            +  + SLL  D +W  N ++  F  +D   ++ I +P     D + W    NG+YS  SG
Sbjct: 1307 EVTINSLLQPDGNWKENEVRSWFHNDDIPWVLGI-KPAINRSDWITWSPSPNGIYSVASG 1366

Query: 122  YKLACSLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRDV 181
            YKL          S++ ++K WW F+W   +  KIK F WR+F +++PT   L KRG  +
Sbjct: 1367 YKLRFQNPNVAECSNKSQIKSWWKFIWGSHLTLKIKNFIWRVFNHWIPTKLELAKRGMVI 1426

Query: 182  SGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADLLWWCWENLAEDR 241
               C  C  + +   HA+W C  ++  W+   F P+  P +++ AAD+LWW  E+L ++ 
Sbjct: 1427 DTHCELCTFQNEDICHALWHCPKVQNIWKLFGF-PKLIPLNLKKAADVLWWLHEHLPKEE 1486

Query: 242  FDEFVALSWWAWSRRNKVVVGGG--DGSV------------------GRRGGVRRIDAQW 301
            F +F+ L+W  W RRN  V      D  +                    +    ++++ W
Sbjct: 1487 FFKFMGLTWLVWQRRNNFVFQHKILDEKIWTSWAMDLIFTHLEPHQKTSKTTAMKLNSAW 1546

Query: 302  VAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIRE 361
            + P    F +NTD       Q   + A++R+ KG +++     IP    V   EA A+  
Sbjct: 1547 IPPPQNYFLINTDASLIAGQQGCGLSAVIRHPKGGLVVAATSFIPGCLSVLLAEANAVHL 1606

Query: 362  SLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCRR 421
             + +A+   +   +V SD+  ++  L S+    ++ G++V++IKLL        F +  R
Sbjct: 1607 GIQLAIRWSITNAQVGSDNHSLIKALNSDTSYPTDWGQLVQDIKLLRNKFQTLQFLFFTR 1666

BLAST of Lag0034425 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 5.3e-27
Identity = 108/450 (24.00%), Postives = 188/450 (41.78%), Query Frame = 0

Query: 10  SYVWKSLLWGRDLLQQGLRWRVGNGKSIRIMEDSWIPRESTLKVIHKTGINPD--AKVES 69
           S+VWKS+   +++L+QG R  VGNG+ I I    W+  +     +    + P   A V S
Sbjct: 108 SFVWKSIHASQEILRQGARAVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSS 167

Query: 70  LLNLDN-------SWNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERNGMYSARS 129
           +L + +        W  ++I+ LF E + + I  +    RR+ D   W +  +G Y+ +S
Sbjct: 168 ILKVSDLIDESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKS 227

Query: 130 GYKLACSL---RGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFFNYLPTLSNLKKR 189
           GY +   +   R +     +  +   +  +W  +   KI+ F W+   N LP    L  R
Sbjct: 228 GYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYR 287

Query: 190 GRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPFYPRPGPPSIQHAADL---LWWCW 249
                  C RC   ++T  H +++C   +  W  S     P P   + A  +   L+W +
Sbjct: 288 HLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSI---PIPLGGEWADSIYVNLYWVF 347

Query: 250 E----NLAEDRFDEFVA-LSWWAWSRRNKVVVGGGD-----------------------G 309
                N   ++  + V  L W  W  RN++V  G +                        
Sbjct: 348 NLGNGNPQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAE 407

Query: 310 SVGRRGGVRRID-AQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLI 369
           S G +  V R    +W  P     K NTD   +R  +   IG ++RN+KGEV     + +
Sbjct: 408 SCGTKPQVNRSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARAL 467

Query: 370 PFVHDVDTLEAMAIRESLVVAMEVGLLALEVESDSARVVALLRSEMEDVSEVGEVVKEIK 416
           P +  V   E  A+R +++         +  ESDS  ++ +L ++ E    +   +++++
Sbjct: 468 PKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQ 527

BLAST of Lag0034425 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-21
Identity = 115/462 (24.89%), Postives = 186/462 (40.26%), Query Frame = 0

Query: 1   MEAGDRGSGSYVWKSLLWGRDLLQQGLRWRVGNGKSIRI----MEDSWIPRESTLKVIHK 60
           ++A  R   SY W SLL G  LL++G R  +G+G++IRI    + DS  PR    +  +K
Sbjct: 12  LDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHPPRPLNTEETYK 71

Query: 61  TGINPDAKVESLLNLDNS---WNVNLIQELFKEEDAEAIVSIPRPRRRVEDKLIWHFERN 120
                +  + +L     S   W+ + I +   + D   I  I   + +  DK+IW++   
Sbjct: 72  -----EMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKIIWNYNTT 131

Query: 121 GMYSARSGYKLAC--------SLRGNVGNSDQRRMKQWWNFLWTRKIPNKIKFFCWRLFF 180
           G Y+ RSGY L          ++    G+ D +        +W   I  K+K F WR   
Sbjct: 132 GEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTR------IWNLPIMPKLKHFLWRALS 191

Query: 181 NYLPTLSNLKKRGRDVSGECPRCRRKEKTGFHAVWECRHIKQCWRESPF----YPRPGPP 240
             L T   L  RG  +   CPRC R+ ++  HA++ C      WR S             
Sbjct: 192 QALATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSND 251

Query: 241 SIQHAADLLWWCWENLAEDRFDEF--VALSWWAWSRRNKVVVGGGDGSVGRR-------- 300
             ++ +++L +  +    D F +   V L W  W  RN VV      S  +         
Sbjct: 252 FEENISNILNFVQDTTMSD-FHKLLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAET 311

Query: 301 --------------GGVRRI---DAQWVAPISPSFKLNTDVGTSRKTQTSSIGAIVRNDK 360
                            R+I     +W  P +   K N D G   +   ++ G I+RN  
Sbjct: 312 HDWLNATQSHKKTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHY 371

Query: 361 G-EVMLTMMKLIPFVHDVDTLEA--MAIRESLVVAMEVGLLALEVESDSARVVALLRSEM 414
           G  +    MKL    H  + LEA   A+  +L      G   + +E D   ++ L+   +
Sbjct: 372 GTPISWGSMKL---AHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING-I 431

BLAST of Lag0034425 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 6.3e-12
Identity = 76/300 (25.33%), Postives = 118/300 (39.33%), Query Frame = 0

Query: 147 LWTRKIPNKIKFFCWRLFFNYLPTLSNLKKRGRDVSGECPRCRRKEKTGFHAVWECRHIK 206
           +W   +  KIK F WR     L T + L+ R  D    C RC  +E+T  H ++ C + +
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 207 QCWRESPFY--PRPGPP-SIQHAADLLWWCWENLAEDRFDEFVA--LSWWAWSRRNKVVV 266
             WR +      + GPP S +   + L    +    +  D F+   + W  W  RN  + 
Sbjct: 97  SVWRSANIIIGNQWGPPSSFEDNLNRLIQLSKTQTTNSLDRFLPFWIMWRLWKSRNVFLF 156

Query: 267 GGGDGS--VGRRGGV----------------------------RRIDAQWVAPISPSFKL 326
                S     R G+                            RR  +QW  P     K 
Sbjct: 157 QQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPEGWVKC 216

Query: 327 NTDVGTSRKTQTSSIGAIVRNDKGEVMLTMMKLIPFVHDVDTLEAMAIRESLVVAMEVGL 386
           N D G ++ +  +  G  +R   G ++L     +         EA+    +L V    GL
Sbjct: 217 NFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSLHAEALGFLHALQVIWAHGL 276

Query: 387 LALEVESDSARVVALLRSEMEDVSEVGEVVKEIKLLARGLPACSFGWCRRSANSAAHAVA 412
             +  ESDS  +V L+ +  ED S +G ++ +I+     LP CS  +  R  NSAA A+A
Sbjct: 277 RYVWFESDSKSLVTLINNG-EDHSLLGTLIYDIRHWMLKLPYCSLEFVNRERNSAADALA 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.11.8e-6132.88uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_023904177.19.0e-6133.33uncharacterized protein LOC112015942 [Quercus suber][more]
XP_030931246.12.2e-5932.73uncharacterized protein LOC115957168 [Quercus lobata][more]
XP_024956542.11.4e-5833.03uncharacterized protein LOC112498908 [Citrus sinensis][more]
XP_030964861.11.9e-5833.63uncharacterized protein LOC115986145 [Quercus lobata][more]
Match NameE-valueIdentityDescription
P0C2F65.6e-1321.93Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR48.8e-6232.88uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803PM687.5e-6131.89Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2N9F2A99.7e-6134.28RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8891 P... [more]
A0A803QQT21.4e-5931.96Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QPB75.3e-5931.15Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G29090.15.3e-2724.00Ribonuclease H-like superfamily protein [more]
AT3G09510.11.2e-2124.89Ribonuclease H-like superfamily protein [more]
AT2G02650.16.3e-1225.33Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 292..414
e-value: 5.2E-21
score: 74.7
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 288..419
e-value: 4.0E-12
score: 48.2
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 116..209
e-value: 8.1E-18
score: 64.9
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 16..374
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 16..374
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 291..412
e-value: 1.20407E-16
score: 73.8876
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 290..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034425.1Lag0034425.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity