Lag0034318 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034318
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr3: 6284720 .. 6285793 (-)
RNA-Seq Expression	Lag0034318
Synteny	Lag0034318

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATTATCTCCACCTTTTCTGCAGCTTCGTTAACAGTGGAAGCATTCACTGGGACCTATGGAATAAACTATGGACGAATTGCAAATAACATACCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCCAATAAGATAAAGAATGTTAGGATTTTTGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGTTAGAAATTGTCATCAGTATACCAAACGAATACCTGAGAGACATGAGTGCAAATGAAGATCATGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCAGAGACCCTTATTTGTGGGATTGCTATAGGTAATGAAGTTTTGGGTGGGAGTGATTTTGAATTATGGTCAGTATTGTTGGGTGCAACCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGATGTAATTCAGATTACTACAGCACATTCCCAAGCCGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCAGAGATAATGTTGCTCAGTATATGAAGCCTCTATTGCAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCTGGAAATATTGATATTAACTATACTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTCACCTGCATTATGATAACATGCTTGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATGAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACATGGAGATGACAATGAAAAAGCAGCTACAGTAGATTATGCACGGACTTACAATTATAATTTGAGGAAACGGCTTGCTAAGAAGAAGGGAACTCCTCTTAGGCCAAAAAATGTGGTAAAGGCATATATCTTTGCATTATTCAATGAGAATTTGAAGTGGGGACCGACTTCTGAGAGAAACTTTGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCATATTCACTATTGTCAACTATTAAGGTATGA

mRNA sequence

ATGGAAATTATCTCCACCTTTTCTGCAGCTTCGTTAACAGTGGAAGCATTCACTGGGACCTATGGAATAAACTATGGACGAATTGCAAATAACATACCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCCAATAAGATAAAGAATGTTAGGATTTTTGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGTTAGAAATTGTCATCAGTATACCAAACGAATACCTGAGAGACATGAGTGCAAATGAAGATCATGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCAGAGACCCTTATTTGTGGGATTGCTATAGGTAATGAAGTTTTGGGTGGGAGTGATTTTGAATTATGGTCAGTATTGTTGGGTGCAACCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGATGTAATTCAGATTACTACAGCACATTCCCAAGCCGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCAGAGATAATGTTGCTCAGTATATGAAGCCTCTATTGCAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCTGGAAATATTGATATTAACTATACTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTCACCTGCATTATGATAACATGCTTGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATGAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACATGGAGATGACAATGAAAAAGCAGCTACAGTAGATTATGCACGGACTTACAATTATAATTTGAGGAAACGGCTTGCTAAGAAGAAGGGAACTCCTCTTAGGCCAAAAAATGTGGTAAAGGCATATATCTTTGCATTATTCAATGAGAATTTGAAGTGGGGACCGACTTCTGAGAGAAACTTTGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCATATTCACTATTGTCAACTATTAAGGTATGA

Coding sequence (CDS)

ATGGAAATTATCTCCACCTTTTCTGCAGCTTCGTTAACAGTGGAAGCATTCACTGGGACCTATGGAATAAACTATGGACGAATTGCAAATAACATACCTTCACCTGATAAAGTTGTTACTCTTCTTAGAGCCAATAAGATAAAGAATGTTAGGATTTTTGATGCTGATCACAGTGTTCTCAAGGCCTTCAGTGGGACAGGGTTAGAAATTGTCATCAGTATACCAAACGAATACCTGAGAGACATGAGTGCAAATGAAGATCATGCCTTGAACTGGGTTAAACAAAATGTGCAGCCCTTCCTTCCAGAGACCCTTATTTGTGGGATTGCTATAGGTAATGAAGTTTTGGGTGGGAGTGATTTTGAATTATGGTCAGTATTGTTGGGTGCAACCAAAAATGTTTATAATGCTGTAAAGAAGCTCAATTTAGATGATGTAATTCAGATTACTACAGCACATTCCCAAGCCGTCTTTTCTAATTCGTTTCCTCCCTCATCGTGTAAATTCAGAGATAATGTTGCTCAGTATATGAAGCCTCTATTGCAGTTCTTCTCACAGATTGGCTCTCCTTTCTGTTTAAATGCATACCCATTTCTGGCCTACATGAGTGACCCTGGAAATATTGATATTAACTATACTCTTTTTCTATCAACAGAAGGGATTTATGATCCTAAAACTCACCTGCATTATGATAACATGCTTGATGCTCAGATCGATGCAGCTTATGCCGCACTGGAAGATGCTGGATATGAAGATATGGAAGTCATAGTTACAGAAACAGGATGGGCTTCACATGGAGATGACAATGAAAAAGCAGCTACAGTAGATTATGCACGGACTTACAATTATAATTTGAGGAAACGGCTTGCTAAGAAGAAGGGAACTCCTCTTAGGCCAAAAAATGTGGTAAAGGCATATATCTTTGCATTATTCAATGAGAATTTGAAGTGGGGACCGACTTCTGAGAGAAACTTTGGACTTTTCAAGCCTGATGGGAGCATTTCATATGATATTGGCTTTCAAGGACTTCAATCTTCATCTGCATATTCACTATTGTCAACTATTAAGGTATGA

Protein sequence

MEIISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIKV

Homology

[Close]

BLAST of Lag0034318 vs. NCBI nr
Match: KAA0050153.1 (glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa] >TYK06409.1 glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa])

HSP 1 Score: 685.3 bits (1767), Expect = 2.9e-193
Identity = 337/357 (94.40%), Postives = 349/357 (97.76%), Query Frame = 0


Query: 1   MEIISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVL 60
           MEIISTF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVL
Sbjct: 1   MEIISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVL 60

Query: 61  KAFSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSD 120
           KAFSGTGLEIVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNEVLGGSD
Sbjct: 61  KAFSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSD 120

Query: 121 FELWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180
           FELW+VLLGA KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL
Sbjct: 121 FELWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180

Query: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDA 240
           LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDA
Sbjct: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDA 240

Query: 241 AYAALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPK 300
           AYAALEDAGY+DMEVIVTETGWASHGDDNEKAAT+D ARTYNYNLRKRLAKKKGTPLRPK
Sbjct: 241 AYAALEDAGYKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPK 300

Query: 301 NVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIKV 358
           NVVKAYIFALFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIKV
Sbjct: 301 NVVKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSACSLLSTIKV 357

[Close]

BLAST of Lag0034318 vs. NCBI nr
Match: KAE8637190.1 (hypothetical protein CSA_021703 [Cucumis sativus])

HSP 1 Score: 681.8 bits (1758), Expect = 3.2e-192
Identity = 334/356 (93.82%), Postives = 348/356 (97.75%), Query Frame = 0


Query: 1   MEIISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVL 60
           MEIISTF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVL
Sbjct: 1   MEIISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVL 60

Query: 61  KAFSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSD 120
           KAFSGTGLEIVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNE+LGGSD
Sbjct: 61  KAFSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEILGGSD 120

Query: 121 FELWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180
           FELW+VLLGA KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL
Sbjct: 121 FELWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180

Query: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDA 240
           LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDA
Sbjct: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDA 240

Query: 241 AYAALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPK 300
           AYAALEDAGY+DMEVIVTETGWASHGD+NEKAAT+D ARTYNYNLRKRLAKKKGTPLRPK
Sbjct: 241 AYAALEDAGYKDMEVIVTETGWASHGDENEKAATIDNARTYNYNLRKRLAKKKGTPLRPK 300

Query: 301 NVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           NVVKAYIFALFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIK
Sbjct: 301 NVVKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSANSLLSTIK 356

[Close]

BLAST of Lag0034318 vs. NCBI nr
Match: XP_038878279.1 (glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.3e-189
Identity = 329/353 (93.20%), Postives = 341/353 (96.60%), Query Frame = 0


Query: 4   ISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAF 63
           I +F  A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAF
Sbjct: 17  IFSFPHALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAF 76

Query: 64  SGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFEL 123
           SGTGLEIVISIPNE L +MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFEL
Sbjct: 77  SGTGLEIVISIPNENLNEMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFEL 136

Query: 124 WSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQF 183
           W+VLLGA KN+YNAVKKLNLD VIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQF
Sbjct: 137 WTVLLGAAKNIYNAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQF 196

Query: 184 FSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYA 243
           FSQIGSPFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDAAYA
Sbjct: 197 FSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYA 256

Query: 244 ALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVV 303
           ALEDAGY+DME+IVTETGWASHGDDNE+AAT+D ARTYNYNLRKRLAKKKGTPLRPKNVV
Sbjct: 257 ALEDAGYKDMEIIVTETGWASHGDDNEEAATIDNARTYNYNLRKRLAKKKGTPLRPKNVV 316

Query: 304 KAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           KAYIFALFNENLKWGPTSERN+GLFKPDGSISYDIGF+GLQ SSAYSLLSTIK
Sbjct: 317 KAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFRGLQPSSAYSLLSTIK 369

[Close]

BLAST of Lag0034318 vs. NCBI nr
Match: XP_008443979.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 328/347 (94.52%), Postives = 339/347 (97.69%), Query Frame = 0


Query: 10  ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLE 69
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAFSGTGLE
Sbjct: 23  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 82

Query: 70  IVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLG 129
           IVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNEVLGGSDFELW+VLLG
Sbjct: 83  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFELWTVLLG 142

Query: 130 ATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 189
           A KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS
Sbjct: 143 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 202

Query: 190 PFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 249
           PFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDAAYAALEDAG
Sbjct: 203 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 262

Query: 250 YEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 309
           Y+DMEVIVTETGWASHGDDNEKAAT+D ARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA
Sbjct: 263 YKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 322

Query: 310 LFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           LFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIK
Sbjct: 323 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSACSLLSTIK 369

[Close]

BLAST of Lag0034318 vs. NCBI nr
Match: XP_031745641.1 (glucan endo-1,3-beta-glucosidase 14-like [Cucumis sativus])

HSP 1 Score: 667.9 bits (1722), Expect = 4.8e-188
Identity = 326/347 (93.95%), Postives = 339/347 (97.69%), Query Frame = 0


Query: 10  ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLE 69
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAFSGTGLE
Sbjct: 22  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 81

Query: 70  IVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLG 129
           IVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNE+LGGSDFELW+VLLG
Sbjct: 82  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEILGGSDFELWTVLLG 141

Query: 130 ATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 189
           A KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS
Sbjct: 142 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 201

Query: 190 PFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 249
           PFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDAAYAALEDAG
Sbjct: 202 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 261

Query: 250 YEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 309
           Y+DMEVIVTETGWASHGD+NEKAAT+D ARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA
Sbjct: 262 YKDMEVIVTETGWASHGDENEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 321

Query: 310 LFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           LFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIK
Sbjct: 322 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSANSLLSTIK 368

[Close]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 1.5e-88
Identity = 159/337 (47.18%), Postives = 226/337 (67.06%), Query Frame = 0


Query: 22  GINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRD 81
           GINYG+IANN+PSP +V  LLR+  I  V+++DAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           MS +   A +W++Q ++P + +T I  I +GNE+   +D  L   LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
            L+  + +T+AHS  + S S+PPSS  F++   QY++PLL F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           W S GD+NE  A+ + A  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 322 ERNFGLFKPDGSISYDIGFQGLQSSSAY-SLLSTIKV 358
           ERN+GLF PDG   Y++G QG      Y S  +TIK+
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKI 366

[Close]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 8.4e-87
Identity = 159/340 (46.76%), Postives = 226/340 (66.47%), Query Frame = 0


Query: 3   IISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKA 62
           ++   S   + +   T + G+NYG+I +N+PSP  V+ L+++     V+++DA+  +LKA
Sbjct: 11  LLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKA 70

Query: 63  FSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFE 122
           FS TG+E +I + NEYL  M  +   AL W+KQNV PFLP T I  I IGNE+L  +D  
Sbjct: 71  FSNTGIEFIIGLGNEYLSKMK-DPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSS 130

Query: 123 LWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182
           L + LL A + V++A+    L D I +TTAHS ++  +SFPPS+ +F+ ++   + P+L+
Sbjct: 131 LTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILE 190

Query: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAY 242
           F  +  SPF +NAYPF AY  +P  + +++ LF   +GI DP T  HYDNML AQIDA Y
Sbjct: 191 FHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVY 250

Query: 243 AALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRL--AKKKGTPLRPK 302
           +AL  AG++ + V ++ETGW S GDD+E  AT + A+ YN NL K +   KK  TPL+P 
Sbjct: 251 SALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPN 310

Query: 303 NVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGF 341
           N +  Y+FALFNENLK GPTSERN+GLFKPDG+ +Y +GF
Sbjct: 311 NDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

[Close]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-80
Identity = 152/351 (43.30%), Postives = 226/351 (64.39%), Query Frame = 0


Query: 3   IISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKA 62
           + S F  A  ++     + GINYG++ANN+P P  V+ LL++     V+++DAD   L+A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 63  FSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFE 122
           F+G+G E+ +++ NEYL  MS +   A  WVK+NVQ +LP T I  I +GNEVL  +   
Sbjct: 69  FAGSGFELTVALGNEYLAQMS-DPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 128

Query: 123 LWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182
           L + L  A ++++ A+    L+  I +TTAHS A+   S+PPS+  FR ++   + P+L 
Sbjct: 129 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 188

Query: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAY 242
           F  + GSP  +NAYPF AY  +P ++ +++ LF   +G  DP ++ HYDNML AQ+DA Y
Sbjct: 189 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 248

Query: 243 AALEDAG--YEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRK-RLAKKKGTPLRP 302
            AL+  G  Y+ + ++V+ETGW S+GD  E  AT D AR YN NL K  ++KK  TP+RP
Sbjct: 249 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 308

Query: 303 KNVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYS 351
           +  +  ++FALFNEN+K GPTSERN+GLF PDG+  Y +   G+++SS +S
Sbjct: 309 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSL---GIKTSSTHS 355

[Close]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 6.1e-77
Identity = 147/326 (45.09%), Postives = 206/326 (63.19%), Query Frame = 0


Query: 22  GINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRD 81
           G+NYG++A+N+P P + V LL++  I+ VR++ AD +++KA +GTG+ IVI   N  +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           ++++ + A  W+  NV PF P + I  I +GNE+L  +D  L + LL A +NV  A++ +
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
           +L   I+++T +S  V  +S PPSS  F       +K +LQF S  GSPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
            SDP    + + LF    G  D KT + Y NM DAQ+DA ++AL+  G+E +E++V ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           WAS GD NE  A+VD A+ YN NL   L    GTPL P   V  YIFAL++ENLK GP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

Query: 322 ERNFGLFKPDGSISYDIGFQGLQSSS 348
           ER FGLFK D S+ YD+G     SSS
Sbjct: 327 ERAFGLFKTDLSMVYDVGLAKSSSSS 352

[Close]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-70
Identity = 135/318 (42.45%), Postives = 197/318 (61.95%), Query Frame = 0


Query: 22  GINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRD 81
           G+NYG++++N+PS    V LL++  I+ VR+F A+ +V+KAF+ TG+EIVI   N  +  
Sbjct: 31  GVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDIPT 90

Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           +++N + A  +VK NV  F P + I  I +GNEVL   D +L S LL A +NV NA+   
Sbjct: 91  LASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNALNAA 150

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
           +L   ++++T H+ AV S S+PPSS  F   +   MK LLQF S   +PF ++ YP+ AY
Sbjct: 151 SLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMISPYPYFAY 210

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
            + P    + + LF    G  D      Y NM DAQ+DA ++AL   G++D+E++V ETG
Sbjct: 211 KNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVAETG 270

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           W   GD NE   ++D A+ Y  NL   L  K GTPL P   +  Y+F+L++E+ K G +S
Sbjct: 271 WPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTGASS 330

Query: 322 ERNFGLFKPDGSISYDIG 340
           E+ FGLFKPDGS +YD+G
Sbjct: 331 EKYFGLFKPDGSTTYDVG 348

[Close]

BLAST of Lag0034318 vs. ExPASy TrEMBL
Match: A0A5A7U2L6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001100 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.4e-193
Identity = 337/357 (94.40%), Postives = 349/357 (97.76%), Query Frame = 0


Query: 1   MEIISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVL 60
           MEIISTF+AA LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVL
Sbjct: 1   MEIISTFTAALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVL 60

Query: 61  KAFSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSD 120
           KAFSGTGLEIVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNEVLGGSD
Sbjct: 61  KAFSGTGLEIVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSD 120

Query: 121 FELWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180
           FELW+VLLGA KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL
Sbjct: 121 FELWTVLLGAAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPL 180

Query: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDA 240
           LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDA
Sbjct: 181 LQFFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDA 240

Query: 241 AYAALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPK 300
           AYAALEDAGY+DMEVIVTETGWASHGDDNEKAAT+D ARTYNYNLRKRLAKKKGTPLRPK
Sbjct: 241 AYAALEDAGYKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPK 300

Query: 301 NVVKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIKV 358
           NVVKAYIFALFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIKV
Sbjct: 301 NVVKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSACSLLSTIKV 357

[Close]

BLAST of Lag0034318 vs. ExPASy TrEMBL
Match: A0A1S3B8U7 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487436 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.1e-189
Identity = 328/347 (94.52%), Postives = 339/347 (97.69%), Query Frame = 0


Query: 10  ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLE 69
           A LTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAFSGTGLE
Sbjct: 23  ALLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSGTGLE 82

Query: 70  IVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLG 129
           IVISIPNE L+DMSANEDHA+NWVKQNVQPFLP+TLICGIAIGNEVLGGSDFELW+VLLG
Sbjct: 83  IVISIPNENLKDMSANEDHAMNWVKQNVQPFLPDTLICGIAIGNEVLGGSDFELWTVLLG 142

Query: 130 ATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 189
           A KNVYNAVKKL+LD +IQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS
Sbjct: 143 AAKNVYNAVKKLDLDGLIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGS 202

Query: 190 PFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 249
           PFCLNAYPFLAYMSDPGNIDINY LFLST+GIYDPKTHLHYDNMLDAQIDAAYAALEDAG
Sbjct: 203 PFCLNAYPFLAYMSDPGNIDINYALFLSTKGIYDPKTHLHYDNMLDAQIDAAYAALEDAG 262

Query: 250 YEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 309
           Y+DMEVIVTETGWASHGDDNEKAAT+D ARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA
Sbjct: 263 YKDMEVIVTETGWASHGDDNEKAATIDNARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFA 322

Query: 310 LFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           LFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA SLLSTIK
Sbjct: 323 LFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSACSLLSTIK 369

[Close]

BLAST of Lag0034318 vs. ExPASy TrEMBL
Match: A0A6J1CUI0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014401 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 323/354 (91.24%), Postives = 336/354 (94.92%), Query Frame = 0


Query: 3   IISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKA 62
           ++  F+ ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKA
Sbjct: 18  LVFFFTLASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKA 77

Query: 63  FSGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFE 122
           FSGTGLEIVISIPNEYL+DMSANED ALNWVKQNVQPFLPET ICGIAIGNEVLGGSD+ 
Sbjct: 78  FSGTGLEIVISIPNEYLKDMSANEDRALNWVKQNVQPFLPETHICGIAIGNEVLGGSDYG 137

Query: 123 LWSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQ 182
           LWS LLGA KNVYNAVKKLNLD +IQITTAHSQAVFSNSFPPSSCKF DNVAQYMKPLL+
Sbjct: 138 LWSALLGAAKNVYNAVKKLNLDGIIQITTAHSQAVFSNSFPPSSCKFGDNVAQYMKPLLE 197

Query: 183 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAY 242
           FFSQIGSPFCLNAYPFLAYMSDPGNIDINY LFLSTEGIYDPKT LHYDNMLDAQIDAAY
Sbjct: 198 FFSQIGSPFCLNAYPFLAYMSDPGNIDINYALFLSTEGIYDPKTDLHYDNMLDAQIDAAY 257

Query: 243 AALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNV 302
           AALEDAGY+DMEVIVTETGWAS GDDNEKAATV+ A+TYNYNLRKRLAKKKGTPLRPKNV
Sbjct: 258 AALEDAGYKDMEVIVTETGWASRGDDNEKAATVNNAQTYNYNLRKRLAKKKGTPLRPKNV 317

Query: 303 VKAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           +KAYIFALFNENLKWGPTSERN+GLFKPDGSISYDIGFQGLQSSSA S  STIK
Sbjct: 318 LKAYIFALFNENLKWGPTSERNYGLFKPDGSISYDIGFQGLQSSSACSPFSTIK 371

[Close]

BLAST of Lag0034318 vs. ExPASy TrEMBL
Match: A0A6J1HB81 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461741 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 321/353 (90.93%), Postives = 339/353 (96.03%), Query Frame = 0


Query: 4   ISTFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAF 63
           +S+F  ASLT+EAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAF
Sbjct: 19  LSSFPPASLTIEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAF 78

Query: 64  SGTGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFEL 123
           SGTGLE+VISIPNEYL+DMSANEDHALNWVKQNVQPFLPETLI GIAIGNEVLGGSDFEL
Sbjct: 79  SGTGLELVISIPNEYLKDMSANEDHALNWVKQNVQPFLPETLIRGIAIGNEVLGGSDFEL 138

Query: 124 WSVLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQF 183
           W+VLLGA KNV+NA+KKL LDD IQITTAHSQAVFSNSFPPSSCKFR+NVAQYMKPLLQF
Sbjct: 139 WTVLLGAAKNVHNALKKLGLDDGIQITTAHSQAVFSNSFPPSSCKFRENVAQYMKPLLQF 198

Query: 184 FSQIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYA 243
           FSQIGSPFCLNAYPFLAYMSDP NIDINY LFLST+GI DPKT LHYDNMLDAQIDAAYA
Sbjct: 199 FSQIGSPFCLNAYPFLAYMSDPENIDINYALFLSTKGIDDPKTRLHYDNMLDAQIDAAYA 258

Query: 244 ALEDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVV 303
           ALEDAGY+DMEVIVTETGWAS GDDNE+AATV  ARTYN+NLRKRLAKKKGTPLRPK+VV
Sbjct: 259 ALEDAGYKDMEVIVTETGWASRGDDNEEAATVGNARTYNFNLRKRLAKKKGTPLRPKHVV 318

Query: 304 KAYIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           KAYIFALFNENLKWGPTSERN+GLFKPDGSI+YDIGFQGLQSSSAY+LLSTIK
Sbjct: 319 KAYIFALFNENLKWGPTSERNYGLFKPDGSIAYDIGFQGLQSSSAYALLSTIK 371

[Close]

BLAST of Lag0034318 vs. ExPASy TrEMBL
Match: A0A6J1JHR0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485203 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.7e-183
Identity = 321/351 (91.45%), Postives = 337/351 (96.01%), Query Frame = 0


Query: 6   TFSAASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSG 65
           +F  ASLT+EAFTGTYGINYGRIANNIPSPDKVVTLLRA KIKNVRI+DADHSVLKAFSG
Sbjct: 21  SFPPASLTIEAFTGTYGINYGRIANNIPSPDKVVTLLRAAKIKNVRIYDADHSVLKAFSG 80

Query: 66  TGLEIVISIPNEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWS 125
           TGLE+VISIPNEYL+DMSANEDHALNWVKQNVQPFLPETLI GIAIGNEVLGGSDFELW+
Sbjct: 81  TGLELVISIPNEYLKDMSANEDHALNWVKQNVQPFLPETLIRGIAIGNEVLGGSDFELWT 140

Query: 126 VLLGATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFS 185
           VLLGA KNV+NA+KKL LDD IQITTAHSQAVFSNSFPPSSCKFR+NVAQYMKPLLQFFS
Sbjct: 141 VLLGAAKNVHNALKKLGLDDGIQITTAHSQAVFSNSFPPSSCKFRENVAQYMKPLLQFFS 200

Query: 186 QIGSPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAAL 245
           QIGSPFCLNAYPFLAYMSDP NIDINY LFLST+GI DPKT LHYDNMLDAQIDAAYAAL
Sbjct: 201 QIGSPFCLNAYPFLAYMSDPENIDINYALFLSTKGIDDPKTRLHYDNMLDAQIDAAYAAL 260

Query: 246 EDAGYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKA 305
           EDAGY+DMEVIVTETGWAS GDDNE+AATV  ARTYNYNLRKRLAKKKGTPLRPK+VVKA
Sbjct: 261 EDAGYKDMEVIVTETGWASRGDDNEEAATVGNARTYNYNLRKRLAKKKGTPLRPKHVVKA 320

Query: 306 YIFALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           YIFALFNENLKWGPTSERN+GLFKPDGSI+YDIGFQGLQSSSAY+LLSTIK
Sbjct: 321 YIFALFNENLKWGPTSERNYGLFKPDGSIAYDIGFQGLQSSSAYALLSTIK 371

[Close]

BLAST of Lag0034318 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-147
Identity = 249/341 (73.02%), Postives = 293/341 (85.92%), Query Frame = 0


Query: 16  AFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIP 75
           AF GTYGINYGRIA+NIPSP+KVV LL+  KI+NVRI+D DH+VL+AFSGTGL++V+ +P
Sbjct: 29  AFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRIYDVDHTVLEAFSGTGLDLVVGLP 88

Query: 76  NEYLRDMSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVY 135
           N +L++MS+N DHA +WVK+N+Q FLP+T I GIAIGNEVLGG D EL   LLGA KNVY
Sbjct: 89  NGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVY 148

Query: 136 NAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNA 195
           NA+KK+NL+D +QITTAHSQAVFS+S+PPSSC F++NV Q+MKPLL+FF QIGSPFCLNA
Sbjct: 149 NALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNA 208

Query: 196 YPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEV 255
           YPFLAY  +P  IDINY LF  TEGIYDPKT LHYDNMLDAQIDAAY AL+DAG++ MEV
Sbjct: 209 YPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEV 268

Query: 256 IVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENL 315
           ++TETGWAS GD +E AAT + ARTYNYNLRKRLAKKKGTPLRPK V+KAYIFALFNEN 
Sbjct: 269 MITETGWASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENS 328

Query: 316 KWGPTSERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           K G +SE +FGLFKPDG+ISYDIGF  L+S S  SL+S+ K
Sbjct: 329 KPGKSSETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSK 369

[Close]

BLAST of Lag0034318 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-131
Identity = 223/340 (65.59%), Postives = 275/340 (80.88%), Query Frame = 0


Query: 10  ASLTVEAFTGTYGINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLE 69
           +S T  AF GTYG+NYGRIA+N+PSPD V TLL++ KI+N RI+DADHSVL AF GTG+E
Sbjct: 32  SSQTAVAFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSVLTAFRGTGIE 91

Query: 70  IVISIPNEYLRDMSANEDHALNWVKQNVQPFL-PETLICGIAIGNEVLGGSDFELWSVLL 129
           I++ + NE+L+D+S  ED A+NW+K+NV+PF+   T I GIA+GNE+LGG+D  LW  LL
Sbjct: 92  IIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGGTDIGLWEALL 151

Query: 130 GATKNVYNAVKKLNLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIG 189
            A KNVY+A+++L L +V+++++ HS+AVF+NS+PPSSC FRD+VA +MKPLL FF QI 
Sbjct: 152 PAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMKPLLAFFWQIQ 211

Query: 190 SPFCLNAYPFLAYMSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDA 249
           SPF +NAYPFLAY SDP  IDINY LF   +GI DPKT LHYDNM DA +DA+YAALE A
Sbjct: 212 SPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMVDASYAALEKA 271

Query: 250 GYEDMEVIVTETGWASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIF 309
           GY  + VIV+ETGWAS GD +E  A+V  ARTYN NLRKRL K+KGTP RP  VV+AY+F
Sbjct: 272 GYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYRPDMVVRAYVF 331

Query: 310 ALFNENLKWGPTSERNFGLFKPDGSISYDIGFQGLQSSSA 349
           ALFNEN K GPTSERNFGLFKPDG+I+YDIG  GL+SSSA
Sbjct: 332 ALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSA 371

[Close]

BLAST of Lag0034318 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 5.6e-118
Identity = 204/275 (74.18%), Postives = 234/275 (85.09%), Query Frame = 0


Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           MS+N DHA +WVK+N+Q FLP+T I GIAIGNEVLGG D EL   LLGA KNVYNA+KK+
Sbjct: 1   MSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKM 60

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
           NL+D +QITTAHSQAVFS+S+PPSSC F++NV Q+MKPLL+FF QIGSPFCLNAYPFLAY
Sbjct: 61  NLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAY 120

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
             +P  IDINY LF  TEGIYDPKT LHYDNMLDAQIDAAY AL+DAG++ MEV++TETG
Sbjct: 121 TYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETG 180

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           WAS GD +E AAT + ARTYNYNLRKRLAKKKGTPLRPK V+KAYIFALFNEN K G +S
Sbjct: 181 WASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSS 240

Query: 322 ERNFGLFKPDGSISYDIGFQGLQSSSAYSLLSTIK 357
           E +FGLFKPDG+ISYDIGF  L+S S  SL+S+ K
Sbjct: 241 ETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSK 275

[Close]

BLAST of Lag0034318 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 328.2 bits (840), Expect = 8.4e-90
Identity = 160/337 (47.48%), Postives = 226/337 (67.06%), Query Frame = 0


Query: 22  GINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRD 81
           GINYG+IANN+PSP +V  LLR+  I  V+++DAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           MS +   A +W++Q ++P + +T I  I +GNE+   +D  L   LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
            L+  + +T+AHS  + S S+PPSS  F++   QY++PLL F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           W S GD+NE  A+ + A  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 322 ERNFGLFKPDGSISYDIGFQGLQSSSAY-SLLSTIKV 358
           ERN+GLF PDG   Y++G QG      Y S  +TIKV
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKV 366

[Close]

BLAST of Lag0034318 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-89
Identity = 159/337 (47.18%), Postives = 226/337 (67.06%), Query Frame = 0


Query: 22  GINYGRIANNIPSPDKVVTLLRANKIKNVRIFDADHSVLKAFSGTGLEIVISIPNEYLRD 81
           GINYG+IANN+PSP +V  LLR+  I  V+++DAD +VL +FS + ++ +I + NEYL++
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 82  MSANEDHALNWVKQNVQPFLPETLICGIAIGNEVLGGSDFELWSVLLGATKNVYNAVKKL 141
           MS +   A +W++Q ++P + +T I  I +GNE+   +D  L   LL A K+VY A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 142 NLDDVIQITTAHSQAVFSNSFPPSSCKFRDNVAQYMKPLLQFFSQIGSPFCLNAYPFLAY 201
            L+  + +T+AHS  + S S+PPSS  F++   QY++PLL F SQI SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 202 MSDPGNIDINYTLFLSTEGIYDPKTHLHYDNMLDAQIDAAYAALEDAGYEDMEVIVTETG 261
              P  + + Y LF   +G+ DP T+LHYDNML AQ+DA Y+A++  G+ D+EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 262 WASHGDDNEKAATVDYARTYNYNLRKRLAKKKGTPLRPKNVVKAYIFALFNENLKWGPTS 321
           W S GD+NE  A+ + A  YN NL K + ++KGTP +    +  Y+FALFNENLK GP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 322 ERNFGLFKPDGSISYDIGFQGLQSSSAY-SLLSTIKV 358
           ERN+GLF PDG   Y++G QG      Y S  +TIK+
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQGYLPDIIYTSRATTIKI 366

The following BLAST results are available for this feature:

BLAST of Lag0034318 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0050153.1	2.9e-193	94.40	glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo var. makuwa] >TYK06409.1 ...	[more]
KAE8637190.1	3.2e-192	93.82	hypothetical protein CSA_021703 [Cucumis sativus]	[more]
XP_038878279.1	3.3e-189	93.20	glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida]	[more]
XP_008443979.1	1.3e-188	94.52	PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo]	[more]
XP_031745641.1	4.8e-188	93.95	glucan endo-1,3-beta-glucosidase 14-like [Cucumis sativus]	[more]

BLAST of Lag0034318 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQG9	1.5e-88	47.18	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8L868	8.4e-87	46.76	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	1.5e-80	43.30	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9M069	6.1e-77	45.09	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q94G86	1.1e-70	42.45	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]

BLAST of Lag0034318 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U2L6	1.4e-193	94.40	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B8U7	6.1e-189	94.52	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487436 PE=3 SV...	[more]
A0A6J1CUI0	2.0e-184	91.24	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014401 ...	[more]
A0A6J1HB81	1.3e-183	90.93	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461741 P...	[more]
A0A6J1JHR0	1.7e-183	91.45	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485203 PE=...	[more]

BLAST of Lag0034318 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26600.1	1.1e-147	73.02	Glycosyl hydrolase superfamily protein	[more]
AT3G15800.1	1.5e-131	65.59	Glycosyl hydrolase superfamily protein	[more]
AT2G26600.2	5.6e-118	74.18	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	8.4e-90	47.48	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.1	1.1e-89	47.18	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 20..346 e-value: 4.6E-117 score: 392.5
None	No IPR available	PANTHER	PTHR32227:SF129	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 13..355
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 22..340 e-value: 6.4E-82 score: 275.6
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 13..355
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 21..339

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034318.1	Lag0034318.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds