Lag0034310 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034310
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cucumber peeling cupredoxin-like
Location	chr3: 6253045 .. 6253620 (-)
RNA-Seq Expression	Lag0034310
Synteny	Lag0034310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCAACAAGGATGACTTCTCTTCCTTCCCTCTTCTTCATTGGAGCTCTTCTGCACGTTGTTTCCGCCGTCGACCACCGCGTCGGAGGCGATTTTGGTTGGAACCAACCGCCAACTCCCACCTTCTTCTCTGAATGGGCTAGTAATAATACTTTCTTTGTCGGAGATTGGCTAAGTAAATATACATATCCACACTTTTTCTAATCATTTATGTTTGATATGTTCTTCTTTTAATTTACACAGAAATTACAAGTGCCATTTTTGTGTGTAGTGTTCAATTCGAGTGCCAATGAAACTCACAACTACGCACGATTCGAGTCTCGAGCAGATTTCGATGGATGCATCAAGCCAGGATTAGTGTTCGAGACTGTAATTTCGATTGGCTTGACTCGTCCTGGTCGTGAATATTTTATGTGTACCATTGGGAACCATTGCAACATGGGCATGAAATTTGCTGTTGACGTTTTGCCAAAGCCTGGAATGCCGCCAAATGCAGCTGTGAAAGTTGGTGCTTTGCAATTGCCTACACTTCTCTTCTCCACCATCATGGCCAGTTTCTTCTTCTTCATTTGA

mRNA sequence

ATGGGTTCAACAAGGATGACTTCTCTTCCTTCCCTCTTCTTCATTGGAGCTCTTCTGCACGTTGTTTCCGCCGTCGACCACCGCGTCGGAGGCGATTTTGGTTGGAACCAACCGCCAACTCCCACCTTCTTCTCTGAATGGGCTAGTAATAATACTTTCTTTGTCGGAGATTGGCTAATGTTCAATTCGAGTGCCAATGAAACTCACAACTACGCACGATTCGAGTCTCGAGCAGATTTCGATGGATGCATCAAGCCAGGATTAGTGTTCGAGACTGTAATTTCGATTGGCTTGACTCGTCCTGGTCGTGAATATTTTATGTGTACCATTGGGAACCATTGCAACATGGGCATGAAATTTGCTGTTGACGTTTTGCCAAAGCCTGGAATGCCGCCAAATGCAGCTGTGAAAGTTGGTGCTTTGCAATTGCCTACACTTCTCTTCTCCACCATCATGGCCAGTTTCTTCTTCTTCATTTGA

Coding sequence (CDS)

ATGGGTTCAACAAGGATGACTTCTCTTCCTTCCCTCTTCTTCATTGGAGCTCTTCTGCACGTTGTTTCCGCCGTCGACCACCGCGTCGGAGGCGATTTTGGTTGGAACCAACCGCCAACTCCCACCTTCTTCTCTGAATGGGCTAGTAATAATACTTTCTTTGTCGGAGATTGGCTAATGTTCAATTCGAGTGCCAATGAAACTCACAACTACGCACGATTCGAGTCTCGAGCAGATTTCGATGGATGCATCAAGCCAGGATTAGTGTTCGAGACTGTAATTTCGATTGGCTTGACTCGTCCTGGTCGTGAATATTTTATGTGTACCATTGGGAACCATTGCAACATGGGCATGAAATTTGCTGTTGACGTTTTGCCAAAGCCTGGAATGCCGCCAAATGCAGCTGTGAAAGTTGGTGCTTTGCAATTGCCTACACTTCTCTTCTCCACCATCATGGCCAGTTTCTTCTTCTTCATTTGA

Protein sequence

MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKFAVDVLPKPGMPPNAAVKVGALQLPTLLFSTIMASFFFFI

Homology

[Close]

BLAST of Lag0034310 vs. NCBI nr
Match: KAG6589838.1 (hypothetical protein SDJN03_15261, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 238.4 bits (607), Expect = 4.2e-59
Identity = 111/160 (69.38%), Postives = 132/160 (82.50%), Query Frame = 0


Query: 1   MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLM 60
           MGSTR + L SL FIGALLH  +AVDHRVGGDFGWN PPTPTFFSEWA+N TF VGD L 
Sbjct: 21  MGSTRSSFLASLLFIGALLHAAAAVDHRVGGDFGWNLPPTPTFFSEWATNTTFSVGDTLR 80

Query: 61  FNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKF 120
           FNSSAN+THNYA  ES+A+FDGC+KPG+ FE VI +   RPGR+YF+C +GNHCN+GMKF
Sbjct: 81  FNSSANQTHNYAMPESQAEFDGCVKPGIFFENVIFVTFDRPGRKYFICNVGNHCNLGMKF 140

Query: 121 AVDVLPKPGMPPNAAVKVGALQLPTL-LFSTIMASFFFFI 160
           AVDVLP PGMPPN+A+K+GA  +PTL + +TI+ +  FFI
Sbjct: 141 AVDVLPSPGMPPNSALKIGA--VPTLFMITTILPNLLFFI 178

[Close]

BLAST of Lag0034310 vs. NCBI nr
Match: XP_022960663.1 (umecyanin-like [Cucurbita moschata])

HSP 1 Score: 238.4 bits (607), Expect = 4.2e-59
Identity = 111/160 (69.38%), Postives = 132/160 (82.50%), Query Frame = 0


Query: 1   MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLM 60
           MGSTR + L SL FIGALLH  +AVDHRVGGDFGWN PPTPTFFSEWA+N TF VGD L 
Sbjct: 1   MGSTRSSFLASLLFIGALLHAAAAVDHRVGGDFGWNLPPTPTFFSEWATNTTFSVGDTLR 60

Query: 61  FNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKF 120
           FNSSAN+THNYA  ES+A+FDGC+KPG+ FE VI +   RPGR+YF+C +GNHCN+GMKF
Sbjct: 61  FNSSANQTHNYAMPESQAEFDGCVKPGIFFENVIFVTFDRPGRKYFICNVGNHCNLGMKF 120

Query: 121 AVDVLPKPGMPPNAAVKVGALQLPTL-LFSTIMASFFFFI 160
           AVDVLP PGMPPN+A+K+GA  +PTL + +TI+ +  FFI
Sbjct: 121 AVDVLPSPGMPPNSALKIGA--VPTLFMITTILPNLLFFI 158

[Close]

BLAST of Lag0034310 vs. NCBI nr
Match: KAG7023509.1 (hypothetical protein SDJN02_14535, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.3 bits (604), Expect = 9.3e-59
Identity = 111/160 (69.38%), Postives = 132/160 (82.50%), Query Frame = 0


Query: 1   MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLM 60
           MGSTR + L SL FIGALLH  +AVDHRVGGDFGWN PPTPTFFSEWA+N TF VGD L 
Sbjct: 1   MGSTRSSFLASLLFIGALLHAAAAVDHRVGGDFGWNLPPTPTFFSEWATNITFSVGDTLR 60

Query: 61  FNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKF 120
           FNSSAN+THNYA  ES+A+FDGC+KPG+ FE VI +   RPGR+YF+C +GNHCN+GMKF
Sbjct: 61  FNSSANQTHNYAMPESQAEFDGCVKPGIFFENVIFVTFDRPGRKYFICNVGNHCNLGMKF 120

Query: 121 AVDVLPKPGMPPNAAVKVGALQLPTL-LFSTIMASFFFFI 160
           AVDVLP PGMPPN+A+K+GA  +PTL + +TI+ +  FFI
Sbjct: 121 AVDVLPSPGMPPNSALKIGA--VPTLFMITTILPNLLFFI 158

[Close]

BLAST of Lag0034310 vs. NCBI nr
Match: XP_023516867.1 (umecyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 227.6 bits (579), Expect = 7.4e-56
Identity = 106/159 (66.67%), Postives = 125/159 (78.62%), Query Frame = 0


Query: 1   MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLM 60
           M STR + L SL FIGALL   +A+DHRVGGDFGWN PPTPTFFSEWA+N TF VGD L 
Sbjct: 1   MASTRSSFLASLLFIGALLQAAAAIDHRVGGDFGWNLPPTPTFFSEWATNTTFSVGDTLR 60

Query: 61  FNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKF 120
           FNSSAN+THNYA  ES+ +FDGC+KPGL FE VI +   RPGR+YF+C +GNHCN+GMKF
Sbjct: 61  FNSSANQTHNYAMPESQPEFDGCVKPGLFFENVIFVTFDRPGRKYFICNVGNHCNLGMKF 120

Query: 121 AVDVLPKPGMPPNAAVKVGALQLPTLLF--STIMASFFF 158
           AVDVLP PGMPPN+A+K+GA   PTL F  +  + +F F
Sbjct: 121 AVDVLPNPGMPPNSALKIGA--FPTLFFIATAFLPNFLF 157

[Close]

BLAST of Lag0034310 vs. NCBI nr
Match: XP_038878652.1 (cucumber peeling cupredoxin-like [Benincasa hispida])

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-55
Identity = 106/160 (66.25%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 1   MGSTRMTSLPSLFFIGALLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLM 60
           + + +M+   S  FI  LL VV+AV HRVGGDFGWN PPTPTFFSEWASN TFFVGD+L+
Sbjct: 2   VSTQKMSFFASFLFIAVLLPVVAAV-HRVGGDFGWNLPPTPTFFSEWASNKTFFVGDFLV 61

Query: 61  FNSSANETHNYARFESRADFDGCIKPGLVFETVISIGLTRPGREYFMCTIGNHCNMGMKF 120
           FNSSANETHNYA  ES+A+FDGC+KPGL FETV S+   RPGR+YF+C +GNHCN+GMKF
Sbjct: 62  FNSSANETHNYAMPESQAEFDGCVKPGLFFETVSSVSFNRPGRKYFICNVGNHCNLGMKF 121

Query: 121 AVDVLPKPG-MPPNAAVKVGALQLPTLLFSTIMASFFFFI 160
           A+D+LPKPG + PNAA+K+ A     LLF TI+ +FFFF+
Sbjct: 122 AIDILPKPGSIAPNAALKIVAFPTLLLLFITIITNFFFFV 160

[Close]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 82.8 bits (203), Expect = 3.8e-15
Identity = 44/122 (36.07%), Postives = 63/122 (51.64%), Query Frame = 0


Query: 27  HRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESRADFDGC--I 86
           H VG + GW+ P +P F+S+WA+  TF VGD L FN  AN  HN    E++  FD C  +
Sbjct: 5   HIVGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPAN-AHNVHEMETKQSFDACNFV 64

Query: 87  KPGLVFETVISI--GLTRPGREYFMCTIGNHCNMGMKFAVDV--------LPKPGMPPNA 137
                 E    +   L   G  YF+CT+G HC+ G K +++V        +P P   P +
Sbjct: 65  NSDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSPPS 124

[Close]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-13
Identity = 46/127 (36.22%), Postives = 64/127 (50.39%), Query Frame = 0


Query: 21  VVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESRADF 80
           VV A D+ VG D  W +P  P F++ WA+  TF VGD L F+ +A   H+ A   S A F
Sbjct: 19  VVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGR-HDVA-VVSEAAF 78

Query: 81  DGCIKPGLVFETV---ISIGLTRPGREYFMCTIGNHCNMGMKFAVDVLPKPGMPPNAAVK 140
           + C K   +       + I L   G +YF+CT+G+HC  G K ++ V+   G    A   
Sbjct: 79  ENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVV-AAGATGGATPG 138

Query: 141 VGALQLP 145
            GA   P
Sbjct: 139 AGATPAP 142

[Close]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-13
Identity = 39/107 (36.45%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 26  DHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESRADFDGCIK 85
           D+ VGGD  W +P  P F+  WA+  TF VGD L F+ +A   H+ A     A FD C K
Sbjct: 2   DYDVGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAG-MHDVAVVTKDA-FDNCKK 61

Query: 86  PGLVFETV---ISIGLTRPGREYFMCTIGNHCNMGMKFAVDVLPKPG 130
              +       + I L   G +Y++CT+G+HC +G K +++V+   G
Sbjct: 62  ENPISHMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGG 106

[Close]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.0e-13
Identity = 43/120 (35.83%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 18  LLHVVSAVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESR 77
           L+ +  A DH +GG  GW           WA+  TF VGD L+F+  A   H+     ++
Sbjct: 17  LIGLTVATDHTIGGPSGWT---VGASLRTWAAGQTFAVGDNLVFSYPA-AFHDVVEV-TK 76

Query: 78  ADFDGC--IKPGLVFETVIS-IGLTRPGREYFMCTIGNHCNMGMKFAVDVLPKPGMPPNA 135
            +FD C  +KP + F    S + LT PG+ YF+C +  HC+ GMK  V+V+P   + P A
Sbjct: 77  PEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATVAPTA 131

[Close]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-10
Identity = 40/106 (37.74%), Postives = 56/106 (52.83%), Query Frame = 0


Query: 24  AVDHRVGGDFGWNQPPTPTFFSEWASNNTFFVGDWLMFNSSANETHNYARFESRADFDGC 83
           A  H+VG   GW     P  +++WAS+N F VGD L+FN + N+ HN  + + +  F  C
Sbjct: 1   ATVHKVGDSTGWT-TLVPYDYAKWASSNKFHVGDSLLFNYN-NKFHNVLQVD-QEQFKSC 60

Query: 84  --IKPGLVFET-VISIGLTRPGREYFMCTIGNHCNMGMKFAVDVLP 127
               P   + +   SI L RPG  YF+C I  HC +G K  + V P
Sbjct: 61  NSSSPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKVDP 103

The following BLAST results are available for this feature:

BLAST of Lag0034310 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6589838.1	4.2e-59	69.38	hypothetical protein SDJN03_15261, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022960663.1	4.2e-59	69.38	umecyanin-like [Cucurbita moschata]	[more]
KAG7023509.1	9.3e-59	69.38	hypothetical protein SDJN02_14535, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023516867.1	7.4e-56	66.67	umecyanin-like [Cucurbita pepo subsp. pepo]	[more]
XP_038878652.1	1.3e-55	66.25	cucumber peeling cupredoxin-like [Benincasa hispida]	[more]

BLAST of Lag0034310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P29602	3.8e-15	36.07	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
Q07488	1.6e-13	36.22	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
P42849	2.8e-13	36.45	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
O82081	8.0e-13	35.83	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
P80728	2.2e-10	37.74	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 22..126 e-value: 4.9E-24 score: 86.6
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 22..125
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 35..118 e-value: 4.5E-14 score: 52.2
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 25..126 score: 26.152925
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 13..133
None	No IPR available	PANTHER	PTHR33021:SF360	OS08G0482600 PROTEIN	coord: 13..133
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 102..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034310.1	Lag0034310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity