Lag0034227 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034227
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase SIS3-like
Location	chr3: 5436827 .. 5440597 (+)
RNA-Seq Expression	Lag0034227
Synteny	Lag0034227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTCGAGGCGTCGATTTCAAGTGGTGAGTCTTCAACGCCGCGCTCTCTAATTCAATGAATTTCTATTTTAACATGCTGTCTGGTTTGGTTGAAGAGAAATACTATGTAGTCAACTTGATAGGTTTTGGACTCCGTATTTGATTCTTGCAGGTATGACGGGTTTTTCTTGTCGATGCTCGCAACTAGTGTGTATCCTTGATTGTTTTGAGGAACTTTAGAATTTCTTGTACGGGATTCTTTTTAGTCTCTTGGAATGGCTGAGTTATGACTATTAACCGATGCGTTTACTTGCGTGGACTGCCTTTCCACTCTTTGAAGCTCTAGTGAATTTGCTCTCTAAGTTTTGCTTTGGCATTGAGAGTTGGAAATCAAACCTAATCATATCATATGCTTTGAAATCTGGATGTTTTTGATTTTTGTATCTTCTTTAACCATTTTGCTCGATAGAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTAAGTTCCACTTAACTTTGAACAAGTATTAGATATTATTACCGTTTTGTTGTTTTCTCGCGATTTTTTTTTGGGTCCAATGATAATCAGTTTTTTTTTTTTTTTTTTTTTTGTACAGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGTAAGGCTTTCAACTTTTAGTTTTACGTCAATTTAGGGTAGCAAAATCTTACGGTTTTTATTTATTTGCTTGTGTATTAAATCCCCATTAGCAAAAAGCTTTACGGAGGTATCCTGTTGTCAGAAAATAAATTAGAGAAAAATGTTCCTTCAATACCTTTAGTATGGGAAGTTTTAATTCTTGGGGGAAATATCAAAACAAACATGGATTCTGACCTGAAGAATTGTCAACCTTTGACCCATCATCAGAAGCATGGTGACAAAGGTGTGAAAAAGAAAAGAAAGAACATTTGTTGCTGATTTAAAGAATTCTGTGCTGTTTTCTAATTTTATTTTATTCTCTTGGGACTGTGACCACGAATGTCCACTTTCTAGATTTGGTAGAATTATTATAAACGTTATTCCCATTTGGGAATTGGACTGCTGCACGAGTTTTGCCATAAAAGTCAGTTACTTTTGACCAAGTAGGTCTTGACGTAAGACCTGTTAAATTTGTTTACCCCTGAATTAATTATTTAGTAAATACATGCACACCTGTTTTTTTTACTCCCCATCTTGTGATAAATATGAGAAAGTTATGATCCTATAATTTGAATATTTTGGTTCTGACTATCATCCCAATTGATGTTCTTAATCTTAATTGCCAATACATTTTCCTTTCGTTTTCAGTATTTCTTGTATAGTTGATGCCATTTGAGATCTTGTCTTCTAAAGTTGTGTGTCATTAGGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTGTATGTTATTTTCTATTTCGACTCCCATCGCCACCACCAGAATCTTCATATTTCTATGAATGTTTATCGAGGTATTGGTTTAAATGTGTCAGCTTCCTGAGCAAGGTCAGAAATGGGGATTCCTAATTTGGTTACTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGGTCAGTTCTATTATGTTTACTATGTAGGTTATTATTGGAGTTTATTATGACTTATGTCTAGTACTCTCCAATAATTTATAGTTTAGCTAACAGTTATTTTTATGGTGCAATTTTTATATATATTTTTTTTCGTGAGTTCTTGTTGAAAATTCTCGAATGTTTTTCTGTATCTATACATGAAATTCACGTGGTGATCTTTGAAATCTCCATGTAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTAAGCAATTAAGCTTCCTGCATCTTAAGATTTTTCAACTGAAAAGACGTGCATCAATTTTTACATTGTCAATTTCTCTACCTTATGTTCACAATGGAATCTATAAAGTAAAGTATAAATCACATTATGTTATATTTATATCGTCTTTAAATTCAATGTTTTGCCCTGGCGATTCTTGAAAATGCAAACACATATGATGGTTTGATCATGAATGAAGTTAAAATGGGGGGAAAACACTTTGAACTGGATAATAGTTCAATCAAAGTCACTAATATTTCTATCCTTACTGTTTTCTTTAGTTCTTTTTAGTATATGATTCTATTCTTAAAAATGAAGATTTGAAAATTTCATTTTCTTGCATCAGCATAACATGTTTAAGGACAATACACTTCTTGGGCAACCAAATAAATATTTAAGTGTATTTTATTCCTTTTTTTACCTCTATAATTTTGCTCATGTGACAATACTGATAGTGACAACAATTTTTTATTACAGGTATTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTGATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCGGCTCAGGTTAGTCTATTAGCACTTGAATTCATCAATATTCTTGAGCTTTCCCCAAACACCTGTTGTCTGGTAGCATAAGGGTAGTTTTGTGCTGTTTTCACTATTTTCCCTCTTAGTGGTTGTACAAATTGTTTGTAAAAGTTTGAGGTAATTTGATTGACGTAAGAGTGTTGTGTATGCTGAAGTTCTTTGCAAAGAGGGCTTAGTGTCATAGGTTGTTGGCCTTTAAACTTTTCAGTTTTCACGAGAGATCTTGTCTAAATCTTAAAGGAATCTGTCTGCCATGTAAAGCCATAGGGTCCGAATTTATGATTGAATCTTTTGTTTTAAATTCTGATGGTCAAGCTATTCGTGGATACTTGGACAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTAATTGATTTTTTGTCTTCATTTAATTTACAGAAGTTCGGAAAACTTTTGGGTGTAAAAAACTATCGTTCATTTATGATTTGTATTTTACTGCTTTTTACGTTCTTTCTGGGACCCTCAAGCTATAGAAGGTGGAGGAATCCTAGATTCATGCTTATAAATATCTGAAAGTATTAATGTTTTGTTGGTGAATTACTGAAAATAAAGTTGCGGGCATTGTTTGTACATTGGTGCAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGCTGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGCTCAGTGTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCTGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAACTCTAG

mRNA sequence

ATGGCTGCTCGAGGCGTCGATTTCAAGTGGTATGACGGGTTTTTCTTGTCGATGCTCGCAACTAGTGTAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTCCTGAGCAAGGTCAGAAATGGGGATTCCTAATTTGGTTACTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTATTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTGATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCGGCTCAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGCTGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGCTCAGTGTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCTGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAACTCTAG

Coding sequence (CDS)

ATGGCTGCTCGAGGCGTCGATTTCAAGTGGTATGACGGGTTTTTCTTGTCGATGCTCGCAACTAGTGTAGTAATAGTTTCGATCAATTGGAAGAAGTATCATACTTGTGTACATCCTTTGCATATATGGATTGTGGTTGATTATACTACCGTCTTTGTTTTTCGCCTGCTGATGTTTGTAGATAATGGACTTGCTGCTGGAATGGGTTTGGACTTTGGGTGGCAGCAGAGGTATGCTCGATTTTGTGGAAGAATTGTGGTCCTTTCAGTTCTTGCGCTTCTGCTATATCCATTTCTTTGGGCTTGGACTGCCATTGGTACTTTATGGTTTAGAGAAGCCCATAAATGTCTTCCTGAGCAAGGTCAGAAATGGGGATTCCTAATTTGGTTACTTTTTAGCTACTGTGCACTCGTATGCATTGCTTGCATGTCATTGGGAAAGTGGTTAACTCGTAGACAAGCACTCTTGTTTCGGGCGCAACAAGGGATCCCTGTATCAGAATATGGGGTATTGGTTGACATGATCCGAGTACCTGATTGGGCTTTTGAAGCTGCAGGACAAGAAACAAGGGTGATGAGCCAAGATGCTGCTACATACCATCCCGGACTTTACTTGACTCCGGCTCAGAGGGAAGCCGTGGAAGCACTTATTGAGGAACTTCCCAAATTCAGGTTAAAGGCTGTTCCAACAGATTGCAGTGAATGTCCTATCTGCTTAGAAGAGTTCCATGTAGGGAATGAGGTTCGTGGCCTCCCTTGTGCTCATAATTTCCATGTAGGCTGTATCGACGAGTGGCTTCGACTGAATGTGAAATGCCCTAGATGTCGTTGCTCAGTGTTCCCAAACCTTGACCTCAGTGCTCTGTCCAATCTTCGTTCTGATACTGATAGGCCATCTGCAAGTGTTGTCACGGCCAATCGTTATGTGAGATCTCAACCTTCCAGCCAAAGTTGCCAAAGTTATCTGGTGAGATTGCAGGGTCTGCTTCGACCTGTTCGCACGGAAAATATTGGTGCACACAGTGATTTAGAGTCTGCTAGTGCTTTGGAAGCTGCTGAGAATGGGTCTGCCTATGACCAAACTATACCAGAACGGTCTCCTGTAAGACCCATGTCTTTGAGTCAGTCCACATCACAACTCTAG

Protein sequence

MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFVDNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQGQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSASVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQTIPERSPVRPMSLSQSTSQL

Homology

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BLAST of Lag0034227 vs. NCBI nr
Match: XP_008453098.1 (PREDICTED: E3 ubiquitin-protein ligase SIS3-like [Cucumis melo])

HSP 1 Score: 744.2 bits (1920), Expect = 5.6e-211
Identity = 363/380 (95.53%), Postives = 367/380 (96.58%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQG PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGFPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELP+FRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPEFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQ 360
           SVVTANRYV SQPSS   QSYLVRLQGLLRPVRTEN  AH DL  ASALEAAENGSA+DQ
Sbjct: 301 SVVTANRYVGSQPSS---QSYLVRLQGLLRPVRTENNDAHGDLGPASALEAAENGSAHDQ 360

Query: 361 TIPERSPVRPMSLSQSTSQL 381
           TIPERSPVRP+SL QSTSQ+
Sbjct: 361 TIPERSPVRPLSLIQSTSQV 377

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BLAST of Lag0034227 vs. NCBI nr
Match: XP_004138113.1 (E3 ubiquitin-protein ligase SIS3 [Cucumis sativus] >KGN63587.1 hypothetical protein Csa_013206 [Cucumis sativus])

HSP 1 Score: 741.1 bits (1912), Expect = 4.7e-210
Identity = 360/380 (94.74%), Postives = 367/380 (96.58%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLATSVVIVSINWK+YHTCVHPLHIWIVVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKRYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLA+LLYPFLWAWTAIGT+WFREAHKCLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLAVLLYPFLWAWTAIGTIWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDM+RVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMVRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQ 360
           SV+TANRYV SQPSS   QSYLVRLQGLLRPVRTE   AH DL  ASALEAAENGSA++Q
Sbjct: 301 SVLTANRYVGSQPSS---QSYLVRLQGLLRPVRTEENDAHGDLGPASALEAAENGSAHEQ 360

Query: 361 TIPERSPVRPMSLSQSTSQL 381
           TIPERSPVRPMSL QSTSQL
Sbjct: 361 TIPERSPVRPMSLIQSTSQL 377

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BLAST of Lag0034227 vs. NCBI nr
Match: XP_038880106.1 (E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida])

HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-209
Identity = 360/380 (94.74%), Postives = 366/380 (96.32%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLATSVV+VSINWK+YHTCVHPLHIWIVVDYT VFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVMVSINWKRYHTCVHPLHIWIVVDYTAVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSD DRPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDVDRPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQ 360
           SVVTAN Y+RSQPSS   QSYLVRLQGLLRPVRTE+  A SDLESA+ALEAAEN S +DQ
Sbjct: 301 SVVTANHYMRSQPSS---QSYLVRLQGLLRPVRTESTDAQSDLESANALEAAENASIHDQ 360

Query: 361 TIPERSPVRPMSLSQSTSQL 381
           TIP RSPVRPMSLSQSTSQL
Sbjct: 361 TIPGRSPVRPMSLSQSTSQL 377

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BLAST of Lag0034227 vs. NCBI nr
Match: XP_023516682.1 (E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731.9 bits (1888), Expect = 2.9e-207
Identity = 357/382 (93.46%), Postives = 366/382 (95.81%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEA---AENGSA 360
           SVVTANRY+R QPSS   QSYLVRLQGLLRPVRTEN  AH DLESA ALEA   AENGS+
Sbjct: 301 SVVTANRYMRPQPSS---QSYLVRLQGLLRPVRTENTVAHGDLESAGALEAAENAENGSS 360

Query: 361 YDQTIPERSPVRPMSLSQSTSQ 380
           +DQTIPERSPVRPMSLSQSTSQ
Sbjct: 361 HDQTIPERSPVRPMSLSQSTSQ 379

[Close]

BLAST of Lag0034227 vs. NCBI nr
Match: XP_022921995.1 (E3 ubiquitin-protein ligase SIS3-like [Cucurbita moschata])

HSP 1 Score: 729.6 bits (1882), Expect = 1.4e-206
Identity = 356/382 (93.19%), Postives = 365/382 (95.55%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEA---AENGSA 360
           SVVTANRY+R QPSS   QSYLVRLQGLLRPVRTEN  AH DLESASALE    AENGS+
Sbjct: 301 SVVTANRYMRPQPSS---QSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSS 360

Query: 361 YDQTIPERSPVRPMSLSQSTSQ 380
           +DQTIPERSPVRPM LSQSTSQ
Sbjct: 361 HDQTIPERSPVRPMPLSQSTSQ 379

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BLAST of Lag0034227 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 3.5e-155
Identity = 265/372 (71.24%), Postives = 303/372 (81.45%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLATSV+IV++NW +Y  C +PLHIWIVVDYTTVF+FR+ MFV
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLA+G+GLDFG QQR A FCGR+VVLSVL+LLLYPFLWAWT IGT WF ++  CLPE+
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC L+CIA + +GKWLTRRQ  L RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQE R +SQDAATYHPGLYLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFH+G+EVRGLPCAHNFHV CID+WLRLNVKCPRCRCSVFP+LDLSALSNL+S      +
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 SVVTAN---RYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSA 360
            V T     RY+RSQP S   +SY +R+Q L+ PV T+           +ALE AENG  
Sbjct: 301 QVNTETSEARYIRSQPQS---ESYFLRVQSLIHPVHTD-----------TALETAENGGV 357

Query: 361 YDQTIPERSPVR 370
               + + SP R
Sbjct: 361 -PPVLTDLSPSR 357

[Close]

BLAST of Lag0034227 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.1e-11
Identity = 36/107 (33.64%), Postives = 53/107 (49.53%), Query Frame = 0


Query: 203 LYLTPAQREAVEALIEELPKFRL-------------KAVPTDC--------------SEC 262
           LY    Q  A +  I+++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 263 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPN 283
            ICL E+  G E+R LPC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]

BLAST of Lag0034227 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.0e-10
Identity = 45/196 (22.96%), Postives = 84/196 (42.86%), Query Frame = 0


Query: 98  FLWAWTAIGTLWFREAHKCLPEQGQK--W------GFLIWLLFSYCALVCIACMSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +  + +A     G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 343 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 402

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 403 ATCPLCKYNILKSSNL 403

[Close]

BLAST of Lag0034227 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.8e-10
Identity = 24/53 (45.28%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 227 AVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSV 280
           A+P + +EC ICL  +    E+R LPC H+FH GC+D+WL +N  CP C+ ++
Sbjct: 317 ALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

[Close]

BLAST of Lag0034227 vs. ExPASy Swiss-Prot
Match: Q9SK92 (E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-10
Identity = 31/73 (42.47%), Postives = 39/73 (53.42%), Query Frame = 0


Query: 215 ALIEELPKFRLKAVPT-----DCSECPICLEEFHVGNEVRGLP-CAHNFHVGCIDEWLRL 274
           ++IE  P F    V T     +  ECP+CL EF     +R +P C H FH GCID WLR 
Sbjct: 93  SVIETFPTFPYSTVKTLRIGKEALECPVCLNEFEDDETLRLIPQCCHVFHPGCIDAWLRS 152

Query: 275 NVKCPRCRCSVFP 282
              CP CR ++ P
Sbjct: 153 QTTCPLCRANLVP 165

[Close]

BLAST of Lag0034227 vs. ExPASy TrEMBL
Match: A0A1S3BVE9 (E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE=4 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 2.7e-211
Identity = 363/380 (95.53%), Postives = 367/380 (96.58%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQG PVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGFPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELP+FRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPEFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQ 360
           SVVTANRYV SQPSS   QSYLVRLQGLLRPVRTEN  AH DL  ASALEAAENGSA+DQ
Sbjct: 301 SVVTANRYVGSQPSS---QSYLVRLQGLLRPVRTENNDAHGDLGPASALEAAENGSAHDQ 360

Query: 361 TIPERSPVRPMSLSQSTSQL 381
           TIPERSPVRP+SL QSTSQ+
Sbjct: 361 TIPERSPVRPLSLIQSTSQV 377

[Close]

BLAST of Lag0034227 vs. ExPASy TrEMBL
Match: A0A0A0LS35 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 PE=4 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.3e-210
Identity = 360/380 (94.74%), Postives = 367/380 (96.58%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLATSVVIVSINWK+YHTCVHPLHIWIVVDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKRYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLA+LLYPFLWAWTAIGT+WFREAHKCLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLAVLLYPFLWAWTAIGTIWFREAHKCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDM+RVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMVRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAYDQ 360
           SV+TANRYV SQPSS   QSYLVRLQGLLRPVRTE   AH DL  ASALEAAENGSA++Q
Sbjct: 301 SVLTANRYVGSQPSS---QSYLVRLQGLLRPVRTEENDAHGDLGPASALEAAENGSAHEQ 360

Query: 361 TIPERSPVRPMSLSQSTSQL 381
           TIPERSPVRPMSL QSTSQL
Sbjct: 361 TIPERSPVRPMSLIQSTSQL 377

[Close]

BLAST of Lag0034227 vs. ExPASy TrEMBL
Match: A0A6J1E7C4 (E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430080 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 6.9e-207
Identity = 356/382 (93.19%), Postives = 365/382 (95.55%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIAC+SLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACVSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEA---AENGSA 360
           SVVTANRY+R QPSS   QSYLVRLQGLLRPVRTEN  AH DLESASALE    AENGS+
Sbjct: 301 SVVTANRYMRPQPSS---QSYLVRLQGLLRPVRTENTVAHCDLESASALEVAENAENGSS 360

Query: 361 YDQTIPERSPVRPMSLSQSTSQ 380
           +DQTIPERSPVRPM LSQSTSQ
Sbjct: 361 HDQTIPERSPVRPMPLSQSTSQ 379

[Close]

BLAST of Lag0034227 vs. ExPASy TrEMBL
Match: A0A6J1JJE6 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485643 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 4.9e-205
Identity = 352/382 (92.15%), Postives = 362/382 (94.76%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MAARGVDFKWYDGFFLSMLA SVV+VSINWK+YHTCVHPLHIWI+VDYTTVFVFRLLMFV
Sbjct: 1   MAARGVDFKWYDGFFLSMLAISVVVVSINWKRYHTCVHPLHIWILVDYTTVFVFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLS+LALLLYPFLWAWTAIGTLWFREAHKCL EQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSILALLLYPFLWAWTAIGTLWFREAHKCLFEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQETRVM QDAA YHPGLYLTPAQREAVEALIEELPKFRLKAVPTDC ECPICLE
Sbjct: 181 AFEAAGQETRVMGQDAAAYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCCECPICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNL SDT+RPSA
Sbjct: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLHSDTERPSA 300

Query: 301 SVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEA---AENGSA 360
           SVVTANRY+R QPSS   QSYLVRLQGLLRPV TEN  AH DLESASALE    AENGS+
Sbjct: 301 SVVTANRYMRPQPSS---QSYLVRLQGLLRPVHTENTVAHGDLESASALEVAENAENGSS 360

Query: 361 YDQTIPERSPVRPMSLSQSTSQ 380
           +DQT+PERSPVRP+S SQST Q
Sbjct: 361 HDQTVPERSPVRPVSSSQSTPQ 379

[Close]

BLAST of Lag0034227 vs. ExPASy TrEMBL
Match: A0A6J1F591 (E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440966 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.2e-203
Identity = 350/382 (91.62%), Postives = 359/382 (93.98%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLATSVVIVSI+WK+YHTCVHPLHIWIVVDYT VF+FRLLMFV
Sbjct: 1   MATRGVDFKWYDGFFLSMLATSVVIVSISWKRYHTCVHPLHIWIVVDYTAVFLFRLLMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLL+PFLW WTAIGTLWF++AH CLPEQ
Sbjct: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLFPFLWVWTAIGTLWFKQAHNCLPEQ 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW
Sbjct: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATY--HPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC 240
           AFEAAG ETRVM QDAATY  HPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC
Sbjct: 181 AFEAAGIETRVMGQDAATYHPHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPIC 240

Query: 241 LEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRP 300
           LEEFH GNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRP
Sbjct: 241 LEEFHEGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRP 300

Query: 301 SASVVTANRYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSAY 360
           SASVVTAN Y+ S P S   QSYLVRLQGLLRPVRTEN G H DLESASA+E  ENGSA+
Sbjct: 301 SASVVTANSYMISHPLS---QSYLVRLQGLLRPVRTENTGTHGDLESASAVEVTENGSAH 360

Query: 361 DQTIPERSPVRPMSLSQSTSQL 381
           DQTIPERSPVRPM LSQST QL
Sbjct: 361 DQTIPERSPVRPMPLSQSTPQL 379

[Close]

BLAST of Lag0034227 vs. TAIR 10
Match: AT3G47990.1 (SUGAR-INSENSITIVE 3 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.5e-156
Identity = 265/372 (71.24%), Postives = 303/372 (81.45%), Query Frame = 0


Query: 1   MAARGVDFKWYDGFFLSMLATSVVIVSINWKKYHTCVHPLHIWIVVDYTTVFVFRLLMFV 60
           MA RGVDFKWYDGFFLSMLATSV+IV++NW +Y  C +PLHIWIVVDYTTVF+FR+ MFV
Sbjct: 1   MAMRGVDFKWYDGFFLSMLATSVIIVAVNWNRYRACEYPLHIWIVVDYTTVFIFRVFMFV 60

Query: 61  DNGLAAGMGLDFGWQQRYARFCGRIVVLSVLALLLYPFLWAWTAIGTLWFREAHKCLPEQ 120
           DNGLA+G+GLDFG QQR A FCGR+VVLSVL+LLLYPFLWAWT IGT WF ++  CLPE+
Sbjct: 61  DNGLASGLGLDFGSQQRNAMFCGRVVVLSVLSLLLYPFLWAWTVIGTQWFTKSKTCLPEE 120

Query: 121 GQKWGFLIWLLFSYCALVCIACMSLGKWLTRRQALLFRAQQGIPVSEYGVLVDMIRVPDW 180
           GQKWGFLIWL+FSYC L+CIA + +GKWLTRRQ  L RAQQGIP+SE+G+LVDMIRVPDW
Sbjct: 121 GQKWGFLIWLMFSYCGLLCIAFICVGKWLTRRQVHLLRAQQGIPISEFGILVDMIRVPDW 180

Query: 181 AFEAAGQETRVMSQDAATYHPGLYLTPAQREAVEALIEELPKFRLKAVPTDCSECPICLE 240
           AFEAAGQE R +SQDAATYHPGLYLTPAQ EAVEALI+ELPKFRLKAVP DC EC ICLE
Sbjct: 181 AFEAAGQEMRGISQDAATYHPGLYLTPAQTEAVEALIQELPKFRLKAVPDDCGECLICLE 240

Query: 241 EFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDRPSA 300
           EFH+G+EVRGLPCAHNFHV CID+WLRLNVKCPRCRCSVFP+LDLSALSNL+S      +
Sbjct: 241 EFHIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCRCSVFPDLDLSALSNLQSSGTEQHS 300

Query: 301 SVVTAN---RYVRSQPSSQSCQSYLVRLQGLLRPVRTENIGAHSDLESASALEAAENGSA 360
            V T     RY+RSQP S   +SY +R+Q L+ PV T+           +ALE AENG  
Sbjct: 301 QVNTETSEARYIRSQPQS---ESYFLRVQSLIHPVHTD-----------TALETAENGGV 357

Query: 361 YDQTIPERSPVR 370
               + + SP R
Sbjct: 361 -PPVLTDLSPSR 357

[Close]

BLAST of Lag0034227 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-12
Identity = 36/107 (33.64%), Postives = 53/107 (49.53%), Query Frame = 0


Query: 203 LYLTPAQREAVEALIEELPKFRL-------------KAVPTDC--------------SEC 262
           LY    Q  A +  I+++PKFR              + + T+C              +EC
Sbjct: 279 LYAVADQEGASKNDIDQMPKFRFTKTGNVEKLSGKARGIMTECGTDSPIERSLSPEDAEC 338

Query: 263 PICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLNVKCPRCRCSVFPN 283
            ICL E+  G E+R LPC H+FH  CID+WL +N +CP C+ ++  N
Sbjct: 339 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNILKN 385

[Close]

BLAST of Lag0034227 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-12
Identity = 37/93 (39.78%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 207 PAQREAVEALIEELPKFRL--KAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDE 266
           P    A E  I  LP  ++  + +  D S+C +C+EEF VG +   LPC H +H  CI  
Sbjct: 194 PGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVP 253

Query: 267 WLRLNVKCPRCRCSVFPNLDLSALSNLRSDTDR 298
           WLRLN  CP CR  + P ++  A S  RS+  R
Sbjct: 254 WLRLNNSCPICRRDL-PLVNTVAESRERSNPIR 285

[Close]

BLAST of Lag0034227 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 69.3 bits (168), Expect = 7.5e-12
Identity = 45/196 (22.96%), Postives = 84/196 (42.86%), Query Frame = 0


Query: 98  FLWAWTAIGTLWFREAHKCLPEQGQK--W------GFLIWLLFSYCALVCIACMSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 223 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 282

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +  + +A     G+      
Sbjct: 283 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 342

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 343 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 402

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 403 ATCPLCKYNILKSSNL 403

[Close]

BLAST of Lag0034227 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 69.3 bits (168), Expect = 7.5e-12
Identity = 45/196 (22.96%), Postives = 84/196 (42.86%), Query Frame = 0


Query: 98  FLWAWTAIGTLWFREAHKCLPEQGQK--W------GFLIWLLFSYCALVCIACMSLGKWL 157
           F + W  IG  W     + L ++  +  W      GF ++ +    AL C+  +++   L
Sbjct: 152 FSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGIAVCCCL 211

Query: 158 TRRQALLFRAQQGIPVSEYGVLVDMIRVPDWAFEAAGQETRVMSQDAATYHPGLYLTPAQ 217
               A+L+     +   E     D+ ++  + F   G   +  + +A     G+      
Sbjct: 212 PCIIAVLY----AVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMTECGT 271

Query: 218 REAVEALIEELPKFRLKAVPTDCSECPICLEEFHVGNEVRGLPCAHNFHVGCIDEWLRLN 277
              +E  + +           + +EC ICL  +  G E+R LPC H+FH  C+D+WL +N
Sbjct: 272 DSPIEHTLLQ-----------EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYIN 331

Query: 278 VKCPRCRCSVFPNLDL 286
             CP C+ ++  + +L
Sbjct: 332 ATCPLCKYNILKSSNL 332

The following BLAST results are available for this feature:

BLAST of Lag0034227 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453098.1	5.6e-211	95.53	PREDICTED: E3 ubiquitin-protein ligase SIS3-like [Cucumis melo]	[more]
XP_004138113.1	4.7e-210	94.74	E3 ubiquitin-protein ligase SIS3 [Cucumis sativus] >KGN63587.1 hypothetical prot...	[more]
XP_038880106.1	1.8e-209	94.74	E3 ubiquitin-protein ligase SIS3-like [Benincasa hispida]	[more]
XP_023516682.1	2.9e-207	93.46	E3 ubiquitin-protein ligase SIS3-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022921995.1	1.4e-206	93.19	E3 ubiquitin-protein ligase SIS3-like [Cucurbita moschata]	[more]

BLAST of Lag0034227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYT9	3.5e-155	71.24	E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV...	[more]
Q93Z92	2.1e-11	33.64	E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116...	[more]
Q9LN71	1.0e-10	22.96	E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127...	[more]
Q8LDB8	1.8e-10	45.28	E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631...	[more]
Q9SK92	2.3e-10	42.47	E3 ubiquitin-protein ligase ATL15 OS=Arabidopsis thaliana OX=3702 GN=ATL15 PE=1 ...	[more]

BLAST of Lag0034227 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BVE9	2.7e-211	95.53	E3 ubiquitin-protein ligase SIS3-like OS=Cucumis melo OX=3656 GN=LOC103493914 PE...	[more]
A0A0A0LS35	2.3e-210	94.74	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004990 P...	[more]
A0A6J1E7C4	6.9e-207	93.19	E3 ubiquitin-protein ligase SIS3-like OS=Cucurbita moschata OX=3662 GN=LOC111430...	[more]
A0A6J1JJE6	4.9e-205	92.15	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1F591	1.2e-203	91.62	E3 ubiquitin-protein ligase SIS3-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of Lag0034227 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47990.1	2.5e-156	71.24	SUGAR-INSENSITIVE 3	[more]
AT4G11680.1	1.5e-12	33.64	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT1G60360.1	3.3e-12	39.78	RING/U-box superfamily protein	[more]
AT1G12760.1	7.5e-12	22.96	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT1G12760.2	7.5e-12	22.96	Zinc finger, C3HC4 type (RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 235..275 e-value: 5.4E-7 score: 39.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 234..276 e-value: 3.3E-13 score: 49.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 235..276 score: 12.622055
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 204..281 e-value: 3.9E-19 score: 70.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 354..380
None	No IPR available	PANTHER	PTHR47179:SF1	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..358
None	No IPR available	CDD	cd16474	RING-H2_RNF111_like	coord: 234..278 e-value: 1.42936E-18 score: 76.5813
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 216..284
IPR044793	E3 ubiquitin-protein ligase SIS3	PANTHER	PTHR47179	E3 UBIQUITIN-PROTEIN LIGASE SIS3	coord: 1..358

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034227.1	Lag0034227.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0010182	sugar mediated signaling pathway
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004842	ubiquitin-protein transferase activity