Lag0033927 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033927
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionBeta-amyrin 28-monooxygenase
Locationchr3: 3003986 .. 3004621 (+)
RNA-Seq ExpressionLag0033927
SyntenyLag0033927
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGCCTGTGTTTCTCTCTGATCTTAATCTTAGTTTCATTTGTCTCTGTGGGCGTGCTCATCCTGTTCTACCGGCACCGGAGGCAGTTCGCGTACCCGAACACGCCGCCGGGCACCATCGGGTACCCGATCGTCGGCGAGACTCTCGAAATGGTTGCGTACGAGAGGAAAGGGGAGTCGGAGAACTTCATCTTCGAACGCGCCGCCAAGTACAAGACGGAGATATTCACCACTTCCGTCCTCGGGGAGCCCACGGCGGCCATGTGCAGCGCCGCCGGCAACAAGCTTCTGTACTCGAACGAGCAGAAGCTTGTGACTCCGTGGTGGCCGGAATCCGCCAACAAAGTTTTTCCGTCTACAATTAAGTACAATACCAACGAAGAAGCGAAGAGGACCAAGAGTTTACTCCCTCAGTTCATCAAGCCCGAAGCGCTTCAGCGATATGTTGGAGTAATGGACACTATTGCTCAAAAGCACTTTGCTTCCCATTGGGAAAATAACAAGGAAATCGTCGTCGCTTCCACTATAAAAGAGTATGTGCTTTCTCTCTCTATCTCCCAATCTCTCGACAAATATAACTTGGTTGCACATTGGTATGCACTATGTATGGATCTTCTTGTATATCACCGTTAA

mRNA sequence

ATGGAGAGCCTGTGTTTCTCTCTGATCTTAATCTTAGTTTCATTTGTCTCTGTGGGCGTGCTCATCCTGTTCTACCGGCACCGGAGGCAGTTCGCGTACCCGAACACGCCGCCGGGCACCATCGGGTACCCGATCGTCGGCGAGACTCTCGAAATGGTTGCGTACGAGAGGAAAGGGGAGTCGGAGAACTTCATCTTCGAACGCGCCGCCAAGTACAAGACGGAGATATTCACCACTTCCGTCCTCGGGGAGCCCACGGCGGCCATGTGCAGCGCCGCCGGCAACAAGCTTCTGTACTCGAACGAGCAGAAGCTTGTGACTCCGTGGTGGCCGGAATCCGCCAACAAAGTTTTTCCGTCTACAATTAAGTACAATACCAACGAAGAAGCGAAGAGGACCAAGAGTTTACTCCCTCAGTTCATCAAGCCCGAAGCGCTTCAGCGATATGTTGGAGTAATGGACACTATTGCTCAAAAGCACTTTGCTTCCCATTGGGAAAATAACAAGGAAATCGTCGTCGCTTCCACTATAAAAGAGTATGTGCTTTCTCTCTCTATCTCCCAATCTCTCGACAAATATAACTTGGTTGCACATTGGTATGCACTATGTATGGATCTTCTTGTATATCACCGTTAA

Coding sequence (CDS)

ATGGAGAGCCTGTGTTTCTCTCTGATCTTAATCTTAGTTTCATTTGTCTCTGTGGGCGTGCTCATCCTGTTCTACCGGCACCGGAGGCAGTTCGCGTACCCGAACACGCCGCCGGGCACCATCGGGTACCCGATCGTCGGCGAGACTCTCGAAATGGTTGCGTACGAGAGGAAAGGGGAGTCGGAGAACTTCATCTTCGAACGCGCCGCCAAGTACAAGACGGAGATATTCACCACTTCCGTCCTCGGGGAGCCCACGGCGGCCATGTGCAGCGCCGCCGGCAACAAGCTTCTGTACTCGAACGAGCAGAAGCTTGTGACTCCGTGGTGGCCGGAATCCGCCAACAAAGTTTTTCCGTCTACAATTAAGTACAATACCAACGAAGAAGCGAAGAGGACCAAGAGTTTACTCCCTCAGTTCATCAAGCCCGAAGCGCTTCAGCGATATGTTGGAGTAATGGACACTATTGCTCAAAAGCACTTTGCTTCCCATTGGGAAAATAACAAGGAAATCGTCGTCGCTTCCACTATAAAAGAGTATGTGCTTTCTCTCTCTATCTCCCAATCTCTCGACAAATATAACTTGGTTGCACATTGGTATGCACTATGTATGGATCTTCTTGTATATCACCGTTAA

Protein sequence

MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPSTIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEYVLSLSISQSLDKYNLVAHWYALCMDLLVYHR
Homology
[Close]
BLAST of Lag0033927 vs. NCBI nr
Match: XP_022134788.1 (beta-amyrin 28-oxidase-like [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 7.4e-80
Identity = 149/182 (81.87%), Postives = 165/182 (90.66%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME+L  SL+++LVSF  V VLILFYRHR QFAYPNTPPG+IGYPI+GETLEMVA+E+KG+
Sbjct: 1   MENLFLSLLILLVSFPCVAVLILFYRHRAQFAYPNTPPGSIGYPIIGETLEMVAHEQKGQ 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            ENFIF R A++KTE+FTTSVLGEPTAA C+AAGNKLL+SNEQKLVTPWWPESANKVFPS
Sbjct: 61  PENFIFGRVARHKTEVFTTSVLGEPTAAFCTAAGNKLLFSNEQKLVTPWWPESANKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           TIK NTN+EAKRTKSLLPQFIKPEALQRYVGVMDTIA  HFAS WEN  EIVVAST+KEY
Sbjct: 121 TIKINTNDEAKRTKSLLPQFIKPEALQRYVGVMDTIAHNHFASSWENKNEIVVASTVKEY 180

Query: 181 VL 183
            L
Sbjct: 181 AL 182

[Close]
BLAST of Lag0033927 vs. NCBI nr
Match: XP_038878724.1 (beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-77
Identity = 138/185 (74.59%), Postives = 167/185 (90.27%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME++C S++++L+ F+S+ + ILFYRH+ QFAYPN PPG+IGYPI+GETL+MVA+ER G+
Sbjct: 1   MENMCISVLILLILFLSLALFILFYRHKAQFAYPNIPPGSIGYPIIGETLQMVAHERNGQ 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            ENF+F R AKYKTEIFTTS+LGEPTAA+CSAAGNK L+SNEQKLVTPWWP+SA+KVFPS
Sbjct: 61  PENFMFGRVAKYKTEIFTTSILGEPTAALCSAAGNKFLFSNEQKLVTPWWPKSADKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           T+KYN+NEEAKR K+LLPQFIKPEAL+RYVGVMD+IAQKHFAS WEN KEIVVAST+K Y
Sbjct: 121 TVKYNSNEEAKRMKNLLPQFIKPEALRRYVGVMDSIAQKHFASMWENKKEIVVASTVKVY 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFYLA 185

[Close]
BLAST of Lag0033927 vs. NCBI nr
Match: XP_038878723.1 (beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 298.5 bits (763), Expect = 4.5e-77
Identity = 138/185 (74.59%), Postives = 167/185 (90.27%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME++C S++++L+ F+S+ + ILFYRH+ QFAYPN PPG+IGYPI+GETL+MVA+ER G+
Sbjct: 1   MENMCISVLILLILFLSLALFILFYRHKAQFAYPNIPPGSIGYPIIGETLQMVAHERNGQ 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            ENF+F R AKYKTEIFTTS+LGEPTAA+CSAAGNK L+SNEQKLVTPWWP+SA+KVFPS
Sbjct: 61  PENFMFGRVAKYKTEIFTTSILGEPTAALCSAAGNKFLFSNEQKLVTPWWPKSADKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           T+KYN+NEEAKR K+LLPQFIKPEAL+RYVGVMD+IAQKHFAS WEN KEIVVAST+K Y
Sbjct: 121 TVKYNSNEEAKRMKNLLPQFIKPEALRRYVGVMDSIAQKHFASMWENKKEIVVASTVKVY 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFYLA 185

[Close]
BLAST of Lag0033927 vs. NCBI nr
Match: XP_022134593.1 (beta-amyrin 28-oxidase-like [Momordica charantia])

HSP 1 Score: 245.4 bits (625), Expect = 4.5e-61
Identity = 121/185 (65.41%), Postives = 145/185 (78.38%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME+L  S +++LVS V  G+L+LF RHR QFAY N PPGT GYPI GE+LEM A  +KGE
Sbjct: 1   MENLLLSAVVVLVSCVCGGLLLLFRRHRAQFAYRNIPPGTSGYPIFGESLEMAATRKKGE 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E F F+R AKYKTE+FTTS++GEP AA CSAAGNKLL+SNE KLVTPWWPESA KVFPS
Sbjct: 61  PEKFFFDRTAKYKTEVFTTSIVGEPAAAFCSAAGNKLLFSNEGKLVTPWWPESAAKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
                 ++EA  T SLLPQFI+PEAL+RYVGVMD++AQ HFASHW+N  +I+VA T+K Y
Sbjct: 121 A-PGPADQEAVETISLLPQFIRPEALKRYVGVMDSMAQLHFASHWDNKSQILVAHTVKYY 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFGLA 184

[Close]
BLAST of Lag0033927 vs. NCBI nr
Match: EOY32256.1 (Cytochrome P450 [Theobroma cacao])

HSP 1 Score: 225.3 bits (573), Expect = 4.8e-55
Identity = 106/199 (53.27%), Postives = 145/199 (72.86%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME +  SL+L+ VSFV++ + +LFYRH+  ++ PN PPG  G PIVGE+LE ++   KG 
Sbjct: 1   MEHIYLSLLLLFVSFVTLSLFVLFYRHKSNYSAPNLPPGKPGLPIVGESLEFLSTGWKGH 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E FIF+R AKY +++F T++LGEP A  C AAGNK L+SNE KLVT WWP S +K+FPS
Sbjct: 61  PEKFIFDRIAKYSSQVFKTNILGEPAAVFCGAAGNKFLFSNENKLVTAWWPSSVDKIFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           +++ ++ EE+K+ + LLPQF+KPEALQRY+G+MDTIAQ+HFAS WEN  E+VV    K Y
Sbjct: 121 SLQTSSKEESKKMRKLLPQFLKPEALQRYIGIMDTIAQRHFASGWENKDEVVVFPLAKRY 180

Query: 181 V--LSLSISQSLDKYNLVA 198
              L+  +  S++  N VA
Sbjct: 181 TFWLACRLFLSIEDPNHVA 199

[Close]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.3e-54
Identity = 100/185 (54.05%), Postives = 131/185 (70.81%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME    SL+LI V  VS+G+ +LFY+HR  F  PN PPG IG+P+VGE+LE ++   KG 
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E FIF+R +KY +E+F TS+LGEP A    AAGNK L+SNE KLV  WWP S +KVFPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           + + ++ EEAK+ + LLPQF KPEALQRY+G+MD IAQ+HFA  W+N  E++V    K +
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFWLA 185

[Close]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 3.1e-52
Identity = 98/185 (52.97%), Postives = 129/185 (69.73%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME    SL+LI V  VS+ + +L Y+HR  F  PN PPG IG+P+VGE+LE ++   KG 
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E FIF+R +KY +E+F TS+LGEP A    AAGNK L+SNE KLV  WWP S +KVFPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           + + ++ EEAK+ + LLPQF+KPEALQRY G+MD IAQ+HFA  W+N  E++V    K +
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFWLA 185

[Close]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 6.4e-50
Identity = 97/194 (50.00%), Postives = 132/194 (68.04%), Query Frame = 0 

Query: 7   SLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESENFIF 66
           SL+L+ V+F+S+ +  +FY+ +      N PPG +GYPI+GE+LE ++   KG  E FIF
Sbjct: 8   SLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPEKFIF 67

Query: 67  ERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPST-IKYN 126
           +R  KY +E+F TS++GE T   C AA NK L+SNE KLVT WWP+S NK+FP+T +  N
Sbjct: 68  DRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSLDSN 127

Query: 127 TNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY--VLS 186
             EE+ + + LLPQF KPEALQRYVGVMD IAQ+HF +HW+N  EI V    K Y  +L+
Sbjct: 128 LKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTFLLA 187

Query: 187 LSISQSLDKYNLVA 198
             +  S++  N VA
Sbjct: 188 CRLFMSVEDENHVA 198

[Close]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 8.1e-45
Identity = 90/181 (49.72%), Postives = 119/181 (65.75%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRR-QFAYPNTPPGTIGYPIVGETLEMVAYERKG 60
           M+     L+ + V FVS+    LFY+ +    +    PPG  G+P++GE+ E ++   KG
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 61  ESENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFP 120
             E FIF+R AKY + +F TS+ G+P AA C AA NK L+SNE KLV  WWP+S NKVFP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 121 STIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKE 180
           S+ + ++ EEA + + LLP F+KPEALQRYVGVMD IA KHF S WEN KE++V    K 
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

[Close]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.4e-43
Identity = 87/182 (47.80%), Postives = 116/182 (63.74%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFA--YPNTPPGTIGYPIVGETLEMVAYERK 60
           ME     L+ + V F+S+    LFY+ +   +  +P  PPG  G+PI+GE+ E ++   K
Sbjct: 1   MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWK 60

Query: 61  GESENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVF 120
           G  E FIF+R  KY + +F TS+ GEP A  C AA NK L+SNE KLV  WWP+S NKVF
Sbjct: 61  GYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVF 120

Query: 121 PSTIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIK 180
           PS+ + ++ EEA + + +LP F KPEALQRY+G+MD IA  HF S WEN  E+VV    K
Sbjct: 121 PSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAK 180

[Close]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match: A0A6J1C307 (beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006973 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.6e-80
Identity = 149/182 (81.87%), Postives = 165/182 (90.66%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME+L  SL+++LVSF  V VLILFYRHR QFAYPNTPPG+IGYPI+GETLEMVA+E+KG+
Sbjct: 1   MENLFLSLLILLVSFPCVAVLILFYRHRAQFAYPNTPPGSIGYPIIGETLEMVAHEQKGQ 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            ENFIF R A++KTE+FTTSVLGEPTAA C+AAGNKLL+SNEQKLVTPWWPESANKVFPS
Sbjct: 61  PENFIFGRVARHKTEVFTTSVLGEPTAAFCTAAGNKLLFSNEQKLVTPWWPESANKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           TIK NTN+EAKRTKSLLPQFIKPEALQRYVGVMDTIA  HFAS WEN  EIVVAST+KEY
Sbjct: 121 TIKINTNDEAKRTKSLLPQFIKPEALQRYVGVMDTIAHNHFASSWENKNEIVVASTVKEY 180

Query: 181 VL 183
            L
Sbjct: 181 AL 182

[Close]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match: A0A6J1C2F1 (beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006822 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.2e-61
Identity = 121/185 (65.41%), Postives = 145/185 (78.38%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME+L  S +++LVS V  G+L+LF RHR QFAY N PPGT GYPI GE+LEM A  +KGE
Sbjct: 1   MENLLLSAVVVLVSCVCGGLLLLFRRHRAQFAYRNIPPGTSGYPIFGESLEMAATRKKGE 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E F F+R AKYKTE+FTTS++GEP AA CSAAGNKLL+SNE KLVTPWWPESA KVFPS
Sbjct: 61  PEKFFFDRTAKYKTEVFTTSIVGEPAAAFCSAAGNKLLFSNEGKLVTPWWPESAAKVFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
                 ++EA  T SLLPQFI+PEAL+RYVGVMD++AQ HFASHW+N  +I+VA T+K Y
Sbjct: 121 A-PGPADQEAVETISLLPQFIRPEALKRYVGVMDSMAQLHFASHWDNKSQILVAHTVKYY 180

Query: 181 VLSLS 186
              L+
Sbjct: 181 TFGLA 184

[Close]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match: A0A061GRY3 (Cytochrome P450 OS=Theobroma cacao OX=3641 GN=TCM_039935 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.3e-55
Identity = 106/199 (53.27%), Postives = 145/199 (72.86%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME +  SL+L+ VSFV++ + +LFYRH+  ++ PN PPG  G PIVGE+LE ++   KG 
Sbjct: 1   MEHIYLSLLLLFVSFVTLSLFVLFYRHKSNYSAPNLPPGKPGLPIVGESLEFLSTGWKGH 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E FIF+R AKY +++F T++LGEP A  C AAGNK L+SNE KLVT WWP S +K+FPS
Sbjct: 61  PEKFIFDRIAKYSSQVFKTNILGEPAAVFCGAAGNKFLFSNENKLVTAWWPSSVDKIFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           +++ ++ EE+K+ + LLPQF+KPEALQRY+G+MDTIAQ+HFAS WEN  E+VV    K Y
Sbjct: 121 SLQTSSKEESKKMRKLLPQFLKPEALQRYIGIMDTIAQRHFASGWENKDEVVVFPLAKRY 180

Query: 181 V--LSLSISQSLDKYNLVA 198
              L+  +  S++  N VA
Sbjct: 181 TFWLACRLFLSIEDPNHVA 199

[Close]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match: A0A5N6RUH0 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020762 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 5.2e-55
Identity = 105/197 (53.30%), Postives = 138/197 (70.05%), Query Frame = 0 

Query: 1   MESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGE 60
           ME    SL+L+ VSFVS+ +  LFY+HR  F  PN PPG  GYP++GE+LE ++   KG 
Sbjct: 1   MEHFYLSLLLLFVSFVSLSLFTLFYKHRAHFTGPNLPPGKTGYPMIGESLEFLSTGWKGH 60

Query: 61  SENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPS 120
            E FIF+R  ++ +EIF TS+LG+P A  C AA NK L+SNE KLV  WWPES NK+FPS
Sbjct: 61  PEKFIFDRMTRFSSEIFKTSLLGQPAAVFCGAACNKFLFSNENKLVVAWWPESVNKIFPS 120

Query: 121 TIKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEY 180
           + + N+NEEA++ + LLPQF KPEAL RY+G+MDTIAQ+HF+S WENNKE+ V    K  
Sbjct: 121 SNQTNSNEEARKMRKLLPQFFKPEALHRYIGIMDTIAQRHFSSSWENNKEVTVFPLAKSL 180

Query: 181 VLSLSISQSLDKYNLVA 198
                I++  D +N +A
Sbjct: 181 EDPTHIARFADPFNALA 197

[Close]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match: A0A7N2MUU5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.5e-54
Identity = 102/200 (51.00%), Postives = 143/200 (71.50%), Query Frame = 0 

Query: 2   ESLCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGES 61
           ++   SL+L+ VSF+++ +  LFYRHR  F  PN PPG +GYP++GE+ E  +   KG  
Sbjct: 3   QNFYLSLLLLFVSFITLSLFFLFYRHRSNFTGPNLPPGKVGYPMIGESFEFFSTGWKGHP 62

Query: 62  ENFIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPST 121
           E FIF+R +KY +E+F TS+LG+P A  C AAGNK L+SNE KLVT WWP+S NKVFPS+
Sbjct: 63  EKFIFDRMSKYSSEVFKTSLLGQPAAVFCGAAGNKFLFSNENKLVTAWWPDSVNKVFPSS 122

Query: 122 IKYNTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEYV 181
           ++ ++ EEAK+ + +LP F+KPEALQRY+G+MDTIAQ+HF S WEN  E++V    K+Y 
Sbjct: 123 LQTSSKEEAKKMRKMLPNFLKPEALQRYIGIMDTIAQRHFKSGWENRDEVLVFPLSKKYT 182

Query: 182 --LSLSISQSLDKYNLVAHW 200
             L+  +  SL+  N +A +
Sbjct: 183 FWLACRLFLSLEDPNHIARF 202

[Close]
BLAST of Lag0033927 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 175.3 bits (443), Expect = 5.4e-44
Identity = 76/181 (41.99%), Postives = 123/181 (67.96%), Query Frame = 0 

Query: 8   LILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESENFIFE 67
           +I++ +S + + +L+L  +H   F+YPN PPG  G P++GE+   ++  R+G  E FI +
Sbjct: 6   MIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFITD 65

Query: 68  RAAKYKTE---IFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPSTIKY 127
           R  ++ +    +F T + G PTA +  A+GNK L++NE KLV  WWP+S NK+FPS+++ 
Sbjct: 66  RVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQT 125

Query: 128 NTNEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEYVLSL 186
           ++ EEA++ + LL QF+KPEAL+RYVGVMD IAQ+HF + W N  +++V    K++  S+
Sbjct: 126 SSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFSI 185

[Close]
BLAST of Lag0033927 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 171.8 bits (434), Expect = 5.9e-43
Identity = 77/179 (43.02%), Postives = 117/179 (65.36%), Query Frame = 0 

Query: 7   SLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESENFIF 66
           ++I + +S +   +L    +H   F YPN PPG IG+P++GETL  ++  R+G  E F+ 
Sbjct: 4   TIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFVT 63

Query: 67  ERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVFPSTIKYNT 126
           +R   + + IF T + G P A +  A+GNK L++NE KLV  WWP+S NK+FPS+ + ++
Sbjct: 64  DRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTSS 123

Query: 127 NEEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEIVVASTIKEYVLSLS 186
            EEA +T+ LL   +KPEAL+RYVGVMD IAQKHF + W N  +++V    K++  S++
Sbjct: 124 KEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182

[Close]
BLAST of Lag0033927 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 7.8e-11
Identity = 49/187 (26.20%), Postives = 91/187 (48.66%), Query Frame = 0 

Query: 4   LCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESEN 63
           + F+  L+L+S ++ G L+L  R R  +     PPG++G P++GET +++   +    E 
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTR--YRRMGLPPGSLGLPLIGETFQLIGAYKTENPEP 60

Query: 64  FIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPES-ANKVFPSTI 123
           FI ER A+Y + +F T + GEPT        N+ +  NE KL    +P S  N +   ++
Sbjct: 61  FIDERVARYGS-VFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSL 120

Query: 124 KYNTNEEAKRTKSLLPQFIKPEALQRYVGV-MDTIAQKHFASHWENNKEIVVASTIKEYV 183
                   KR  SL   F     ++ ++ + +D + + +  S W  +  +++    K+  
Sbjct: 121 LLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDS-W--SSRVLLMEEAKKIT 180

Query: 184 LSLSISQ 189
             L++ Q
Sbjct: 181 FELTVKQ 181

[Close]
BLAST of Lag0033927 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 7.8e-11
Identity = 49/187 (26.20%), Postives = 91/187 (48.66%), Query Frame = 0 

Query: 4   LCFSLILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESEN 63
           + F+  L+L+S ++ G L+L  R R  +     PPG++G P++GET +++   +    E 
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTR--YRRMGLPPGSLGLPLIGETFQLIGAYKTENPEP 60

Query: 64  FIFERAAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPES-ANKVFPSTI 123
           FI ER A+Y + +F T + GEPT        N+ +  NE KL    +P S  N +   ++
Sbjct: 61  FIDERVARYGS-VFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSL 120

Query: 124 KYNTNEEAKRTKSLLPQFIKPEALQRYVGV-MDTIAQKHFASHWENNKEIVVASTIKEYV 183
                   KR  SL   F     ++ ++ + +D + + +  S W  +  +++    K+  
Sbjct: 121 LLMKGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDS-W--SSRVLLMEEAKKIT 180

Query: 184 LSLSISQ 189
             L++ Q
Sbjct: 181 FELTVKQ 181

[Close]
BLAST of Lag0033927 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 48/208 (23.08%), Postives = 90/208 (43.27%), Query Frame = 0 

Query: 9   ILILVSFVSVGVLILFYRHRRQFAYPNTPPGTIGYPIVGETLEMVAYERKGESENFIFER 68
           +LI VS + + +    Y  R        PPG++G+P++GE+++     +  +   FI ER
Sbjct: 4   LLIWVSLLLISITHWVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIPPFIKER 63

Query: 69  AAKYKTEIFTTSVLGEPTAAMCSAAGNKLLYSNEQKLVTPWWPESANKVF-PSTIKYNTN 128
             KY   IF T+++G P      A  +  +++ E +    W+P++   +F    +     
Sbjct: 64  VKKY-GPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVGSLHG 123

Query: 129 EEAKRTKSLLPQFIKPEALQRYVGVMDTIAQKHFASHWENNKEI----VVASTIKEYVLS 188
              K  K+++      + L++ +  ++  A K     W N   +      AS I +    
Sbjct: 124 FMYKYLKNMVLTLFGHDGLKKMLPQVEMTANKRL-ELWSNQDSVELKDATASMIFDLTAK 183

Query: 189 LSISQSLDK--YNLVAHWYALCMDLLVY 210
             IS   DK   NL A++ A    L+ +
Sbjct: 184 KLISHDPDKSSENLRANFVAFIQGLISF 209

The following BLAST results are available for this feature:
BLAST of Lag0033927 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022134788.17.4e-8081.87beta-amyrin 28-oxidase-like [Momordica charantia][more]
XP_038878724.14.5e-7774.59beta-amyrin 28-monooxygenase-like isoform X2 [Benincasa hispida][more]
XP_038878723.14.5e-7774.59beta-amyrin 28-monooxygenase-like isoform X1 [Benincasa hispida][more]
XP_022134593.14.5e-6165.41beta-amyrin 28-oxidase-like [Momordica charantia][more]
EOY32256.14.8e-5553.27Cytochrome P450 [Theobroma cacao][more]
BLAST of Lag0033927 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F6H9N67.3e-5454.05Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1[more]
A5BFI43.1e-5252.97Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2[more]
Q2MJ206.4e-5050.00Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... [more]
A0A0S2II388.1e-4549.72Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... [more]
I7C6E84.4e-4347.80Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1[more]
BLAST of Lag0033927 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C3073.6e-8081.87beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006973 PE=4 ... [more]
A0A6J1C2F12.2e-6165.41beta-amyrin 28-oxidase-like OS=Momordica charantia OX=3673 GN=LOC111006822 PE=3 ... [more]
A0A061GRY32.3e-5553.27Cytochrome P450 OS=Theobroma cacao OX=3641 GN=TCM_039935 PE=3 SV=1[more]
A0A5N6RUH05.2e-5553.30Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_020762 PE=3 SV=1[more]
A0A7N2MUU51.5e-5451.00Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
BLAST of Lag0033927 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G36110.15.4e-4441.99cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT5G36140.15.9e-4343.02cytochrome P450, family 716, subfamily A, polypeptide 2 [more]
AT5G05690.17.8e-1126.20Cytochrome P450 superfamily protein [more]
AT5G05690.27.8e-1126.20Cytochrome P450 superfamily protein [more]
AT1G12740.12.1e-0823.08cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..206
e-value: 1.8E-24
score: 88.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 28..188
NoneNo IPR availablePANTHERPTHR24286:SF297BETA-AMYRIN 28-OXIDASE-LIKEcoord: 8..185
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033927.1Lag0033927.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen