Lag0033879 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033879 vs. NCBI nr
Match: TYK28576.1 (macrophage migration inhibitory factor-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 189.1 bits (479), Expect = 3.2e-44
Identity = 111/161 (68.94%), Postives = 119/161 (73.91%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MPTLNL+TNVPVD VVAADILKDASK +SKILGKPESV        F      +  L  V
Sbjct: 1   MPTLNLFTNVPVDTVVAADILKDASKAVSKILGKPESVK-------FSAPFPSSLSLLLV 60

Query: 61  LELRFATARLIAGLNFGFS---LLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSV 120
           L L  A   L  G N  FS   + YVMILLNGGTPIVF+ TEE AAYGE+I+IGGLGP V
Sbjct: 61  LALE-ACLWLFKGTNLHFSSSMVQYVMILLNGGTPIVFATTEEPAAYGELISIGGLGPGV 120

Query: 121 NGKLSSTIAEILQTKLHIDSSRFYIKFYDVQASFSRPSKVL 159
           NGKLSSTIAEILQTK+ ID SRFYIKFYDVQASFSRP  VL
Sbjct: 121 NGKLSSTIAEILQTKIQIDGSRFYIKFYDVQASFSRPFNVL 153

BLAST of Lag0033879 vs. NCBI nr
Match: KAA0058211.1 (macrophage migration inhibitory factor-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 188.7 bits (478), Expect = 4.2e-44
Identity = 110/161 (68.32%), Postives = 119/161 (73.91%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MPTLNL+TNVPVD VVAADILKDASK +SKILGKPESV        F      +  L  V
Sbjct: 1   MPTLNLFTNVPVDTVVAADILKDASKAVSKILGKPESVK-------FSAPFPSSLSLLLV 60

Query: 61  LELRFATARLIAGLNFGFS---LLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSV 120
           L L  A   L  G N  FS   + YVMILLNGGTPI+F+ TEE AAYGE+I+IGGLGP V
Sbjct: 61  LALE-ACLWLFKGTNLHFSSSMVQYVMILLNGGTPIIFATTEEPAAYGELISIGGLGPGV 120

Query: 121 NGKLSSTIAEILQTKLHIDSSRFYIKFYDVQASFSRPSKVL 159
           NGKLSSTIAEILQTK+ ID SRFYIKFYDVQASFSRP  VL
Sbjct: 121 NGKLSSTIAEILQTKIQIDGSRFYIKFYDVQASFSRPFNVL 153

BLAST of Lag0033879 vs. NCBI nr
Match: KAG7021698.1 (Macrophage migration inhibitory factor-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 187.6 bits (475), Expect = 9.4e-44
Identity = 107/166 (64.46%), Postives = 115/166 (69.28%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MPTLNL+TN+PVDAVVAADILKDASK +SKILGK ES                       
Sbjct: 1   MPTLNLFTNLPVDAVVAADILKDASKAVSKILGKSES----------------------- 60

Query: 61  LELRFATARLIAGLNFGFSLLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVNGK 120
                                YVMILLNG TPIVF+GTEE AAYGE+I+IGGLGPSVNGK
Sbjct: 61  ---------------------YVMILLNGSTPIVFAGTEEPAAYGELISIGGLGPSVNGK 120

Query: 121 LSSTIAEILQTKLHIDSSRFYIKFYDVQASFSRPSKVLSTCIKGQI 167
           LSSTIAEI+QTKLHIDSSRFYIKF DVQASFSRPSK+LST IKG I
Sbjct: 121 LSSTIAEIIQTKLHIDSSRFYIKFSDVQASFSRPSKLLSTYIKGWI 122

BLAST of Lag0033879 vs. NCBI nr
Match: KAF8392811.1 (hypothetical protein HHK36_021048 [Tetracentron sinense])

HSP 1 Score: 173.3 bits (438), Expect = 1.8e-39
Identity = 94/153 (61.44%), Postives = 119/153 (77.78%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MPTLNL+TNVPVDAVVA+DIL+DA+K ++KI+GKPESV +  ++  F  I L  +  S V
Sbjct: 273 MPTLNLFTNVPVDAVVASDILRDATKAVAKIIGKPESVCTKSSNPFFPLIGLLLYMRSFV 332

Query: 61  LELRFATARLIAGLNFGFSLL--YVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVN 120
             L      L+   ++ + ++  YVMIL+NGG PI F+GTEE AAYGE+I+IGGLGPSVN
Sbjct: 333 GLLCSKIVALLFLYSYIWVMMVQYVMILINGGVPIAFAGTEEPAAYGELISIGGLGPSVN 392

Query: 121 GKLSSTIAEILQTKLHIDSSRFYIKFYDVQASF 152
           GKLSSTIAEIL+TKL +DS+RFYIKFYDVQ S+
Sbjct: 393 GKLSSTIAEILETKLSVDSARFYIKFYDVQVSY 425

BLAST of Lag0033879 vs. NCBI nr
Match: KAE9462921.1 (hypothetical protein C3L33_05176, partial [Rhododendron williamsianum])

HSP 1 Score: 169.9 bits (429), Expect = 2.0e-38
Identity = 94/153 (61.44%), Postives = 111/153 (72.55%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MPTLNL+TNVPVDAVV +DILKDA+K ++KI+GKPESVN  +      +    T  L+  
Sbjct: 1   MPTLNLFTNVPVDAVVTSDILKDATKAVAKIIGKPESVNKENP----QKHKKPTEALACA 60

Query: 61  LELRFATARL-IAGLNFGFSLLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVNG 120
              RF    +      F F L YVMI+LN   PI F+GTEE AAYGE+I+IGGLGPSVNG
Sbjct: 61  CACRFTVVTVKFLSFFFNFILQYVMIVLNASVPIAFAGTEEPAAYGELISIGGLGPSVNG 120

Query: 121 KLSSTIAEILQTKLHIDSSRFYIKFYDVQASFS 153
           +LSSTIA+ILQTKL IDSSRFYIKFYDVQ + S
Sbjct: 121 QLSSTIADILQTKLSIDSSRFYIKFYDVQVTIS 149

BLAST of Lag0033879 vs. ExPASy Swiss-Prot
Match: P81748 (Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 1.3e-08
Identity = 42/150 (28.00%), Postives = 64/150 (42.67%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MP     TNVP +  ++ D LK  SK ++ +LGKPES                       
Sbjct: 1   MPIFTFSTNVPSEN-ISVDFLKSTSKLIAGMLGKPES----------------------- 60

Query: 61  LELRFATARLIAGLNFGFSLLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVNGK 120
                                YV + +NGG  I F GT+  A +G+++++GG+G   N  
Sbjct: 61  ---------------------YVAVHINGGQKITFGGTDAPAGFGQLLSLGGVGGEKNRS 105

Query: 121 LSSTIAEILQTKLHIDSSRFYIKFYDVQAS 151
            S+ + + L   L I  +R YI F D++ S
Sbjct: 121 HSAKLFKHLTDGLGIPGNRMYINFVDMRGS 105

BLAST of Lag0033879 vs. ExPASy Swiss-Prot
Match: O44786 (Macrophage migration inhibitory factor homolog OS=Wuchereria bancrofti OX=6293 GN=MIF PE=3 SV=3)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 43/150 (28.67%), Postives = 64/150 (42.67%), Query Frame = 0

Query: 1   MPTLNLYTNVPVDAVVAADILKDASKPLSKILGKPESVNSGHAHMVFDEITLRTWRLSQV 60
           MP   + TN P D++ +A  LK A   + K LGKPES                       
Sbjct: 1   MPYFTIDTNKPQDSISSA-FLKKAPNVVPKALGKPES----------------------- 60

Query: 61  LELRFATARLIAGLNFGFSLLYVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVNGK 120
                                YV I +NGG P+VF G+E+      + +IG +GP VN  
Sbjct: 61  ---------------------YVSIHVNGGQPMVFGGSEDPCPVCVLKSIGCVGPKVNNS 105

Query: 121 LSSTIAEILQTKLHIDSSRFYIKFYDVQAS 151
            +  + ++L  +L I  +R YI+  D++AS
Sbjct: 121 HAEKLYKLLADELKIPKNRCYIESVDIEAS 105

BLAST of Lag0033879 vs. ExPASy Swiss-Prot
Match: P81529 (Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 PE=1 SV=2)

HSP 1 Score: 45.8 bits (107), Expect = 5.8e-04
Identity = 23/66 (34.85%), Postives = 39/66 (59.09%), Query Frame = 0

Query: 82  YVMILLNGGTPIVFSGTEESAAYGEMIAIGGLGPSVNGKLSSTIAEILQTKLHIDSSRFY 141
           YV + +N    + F G+ + AA+G +++IGG+ PS N   S+ + + L  KL I  +R Y
Sbjct: 37  YVAVHINTDQQLSFGGSTKPAAFGTLMSIGGIEPSRNRDHSAKLFDHLNKKLGIPKNRMY 96

Query: 142 IKFYDV 148
           I F ++
Sbjct: 97  IHFVNL 102