Lag0033836 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033836
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	nicotianamine synthase-like
Location	chr3: 2184560 .. 2185417 (-)
RNA-Seq Expression	Lag0033836
Synteny	Lag0033836

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCCCTCCAGATCCATGGCACCGAAACCGAAATCTCTGCCGAGTTCCTTATAGCTCGAATCATCGATCTCCATGAAAGCATCTCAAAGCTCGAAACCCTAAAGCCCTGCAAGAAAGTGAACACCCTCTTCACCAACCTCGTCAACTTATGCATCTTACCTTCTTCCATAGACGTTTCCACATTGCCACACCATTTACAAGCCATTCGCGAGAGCCTCATGGTTCTGTGCGGCCGAGCCGAGGGCCTACTTGAGTTCGAATTCTCGACCTTGCTCGGAAAAATCCCCAAACCTCTCAACAATCTCACTCTATTTCCATATTACCAGAACTACATCAAATTAGCCCACCTCGAACACAAGATTTTGAATGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGATCGGGTCCTTTGCCTCTAACTTCCATTATCATGGCCATGCAGCACATGAAGACCACGTATTTCGATAATTACGACATTGACAGCAAGGCAAATGACGTGGCGAGGAAGATGGTGGCGTCGGATGCTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGAGATATTGTTGAGGTAAAGGAAGAGCTTGAGGGATATGATTGTGTGTTTTTGGCAGCTCTTGTAGGGATGAAGAAGGAAGAAAAAGTGAAGATTTTAGGGCATTTGAGAAAGTATATGAAAGAAGGTGGGATTTTGGTTGTGAGAAGTGCTAAAGGAGCTAGAGCATTTTTGTACCCTGTGGTTGAGCTTCATGATCTGGTCGGCTTTGAAGTTCTCTCTATTTTTCATCCCACTGATGATGTTGTCAACTCGGTTCTCTTAGCTCGCAAGCCTATAATTATATGA

mRNA sequence

ATGGCAGCCCTCCAGATCCATGGCACCGAAACCGAAATCTCTGCCGAGTTCCTTATAGCTCGAATCATCGATCTCCATGAAAGCATCTCAAAGCTCGAAACCCTAAAGCCCTGCAAGAAAGTGAACACCCTCTTCACCAACCTCGTCAACTTATGCATCTTACCTTCTTCCATAGACGTTTCCACATTGCCACACCATTTACAAGCCATTCGCGAGAGCCTCATGGTTCTGTGCGGCCGAGCCGAGGGCCTACTTGAGTTCGAATTCTCGACCTTGCTCGGAAAAATCCCCAAACCTCTCAACAATCTCACTCTATTTCCATATTACCAGAACTACATCAAATTAGCCCACCTCGAACACAAGATTTTGAATGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGATCGGGTCCTTTGCCTCTAACTTCCATTATCATGGCCATGCAGCACATGAAGACCACGTATTTCGATAATTACGACATTGACAGCAAGGCAAATGACGTGGCGAGGAAGATGGTGGCGTCGGATGCTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGAGATATTGTTGAGGTAAAGGAAGAGCTTGAGGGATATGATTGTGTGTTTTTGGCAGCTCTTGTAGGGATGAAGAAGGAAGAAAAAGTGAAGATTTTAGGGCATTTGAGAAAGTATATGAAAGAAGGTGGGATTTTGGTTGTGAGAAGTGCTAAAGGAGCTAGAGCATTTTTGTACCCTGTGGTTGAGCTTCATGATCTGGTCGGCTTTGAAGTTCTCTCTATTTTTCATCCCACTGATGATGTTGTCAACTCGGTTCTCTTAGCTCGCAAGCCTATAATTATATGA

Coding sequence (CDS)

ATGGCAGCCCTCCAGATCCATGGCACCGAAACCGAAATCTCTGCCGAGTTCCTTATAGCTCGAATCATCGATCTCCATGAAAGCATCTCAAAGCTCGAAACCCTAAAGCCCTGCAAGAAAGTGAACACCCTCTTCACCAACCTCGTCAACTTATGCATCTTACCTTCTTCCATAGACGTTTCCACATTGCCACACCATTTACAAGCCATTCGCGAGAGCCTCATGGTTCTGTGCGGCCGAGCCGAGGGCCTACTTGAGTTCGAATTCTCGACCTTGCTCGGAAAAATCCCCAAACCTCTCAACAATCTCACTCTATTTCCATATTACCAGAACTACATCAAATTAGCCCACCTCGAACACAAGATTTTGAATGACAATGGAGTTGTGAATCCAAAAAAGGTAGCTTTTGTGGGATCGGGTCCTTTGCCTCTAACTTCCATTATCATGGCCATGCAGCACATGAAGACCACGTATTTCGATAATTACGACATTGACAGCAAGGCAAATGACGTGGCGAGGAAGATGGTGGCGTCGGATGCTGAGCTGGAGGGAAGAATGAAGTTTTGGACGAGAGATATTGTTGAGGTAAAGGAAGAGCTTGAGGGATATGATTGTGTGTTTTTGGCAGCTCTTGTAGGGATGAAGAAGGAAGAAAAAGTGAAGATTTTAGGGCATTTGAGAAAGTATATGAAAGAAGGTGGGATTTTGGTTGTGAGAAGTGCTAAAGGAGCTAGAGCATTTTTGTACCCTGTGGTTGAGCTTCATGATCTGGTCGGCTTTGAAGTTCTCTCTATTTTTCATCCCACTGATGATGTTGTCAACTCGGTTCTCTTAGCTCGCAAGCCTATAATTATATGA

Protein sequence

MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDVSTLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII

Homology

[Close]

BLAST of Lag0033836 vs. NCBI nr
Match: XP_038880344.1 (nicotianamine synthase-like [Benincasa hispida])

HSP 1 Score: 499.2 bits (1284), Expect = 2.3e-137
Identity = 251/285 (88.07%), Postives = 268/285 (94.04%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAALQIHGT+TEIS EFL+ RIIDLHESISKLETLKPCKKVN LFTNLVNLCILP SIDV
Sbjct: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP +LQAIRESL+VLCG+AEGLLE EFSTLL KIPKPLNNLTLFPYYQNYIKLA+LE+
Sbjct: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KILNDNG+ NPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYDIDS ANDVA K+V SD 
Sbjct: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEVKEEL GYDCVFLAALVGMKKE+KVKI+ HLRKYMKEGGILVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           AKG RAFLYPVVE+ DLVGFE+LS+FHPTDDVVNSV+LARKP+I+
Sbjct: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 285

[Close]

BLAST of Lag0033836 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 484.2 bits (1245), Expect = 7.8e-133
Identity = 241/285 (84.56%), Postives = 265/285 (92.98%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAALQI GTET+IS EFLIAR+IDLH SIS+LETLKPCK+VN LFTNLV LCILP SIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STL  HLQAIRESL++LCGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+ KE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           A+G RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI++
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Lag0033836 vs. NCBI nr
Match: XP_022987937.1 (nicotianamine synthase-like [Cucurbita maxima])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 242/285 (84.91%), Postives = 263/285 (92.28%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           M ALQI GTET+IS E LIAR+IDLH SIS+LETLKPCK+VN LFTNLV  CILP SIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP HLQAIRESL++ CGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDIDS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+KKE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           AKG RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI+I
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Lag0033836 vs. NCBI nr
Match: KAG7023395.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 480.7 bits (1236), Expect = 8.6e-132
Identity = 239/285 (83.86%), Postives = 263/285 (92.28%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           M AL I GTET+IS EFLIAR+IDLH SIS+LETLKPCK+VN LFTNLV LCILP SIDV
Sbjct: 1   MTALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STL  HLQAIRESL++LCGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+ KE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           A+G RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI++
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Lag0033836 vs. NCBI nr
Match: XP_022922054.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 480.7 bits (1236), Expect = 8.6e-132
Identity = 240/285 (84.21%), Postives = 264/285 (92.63%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAAL I GTET+IS EFLIAR+IDLH SIS+LETLKPCK+VN LFT+LV LCILP SIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STL  HLQAIRESL++LCGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+ KE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           AKG RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI++
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 6.1e-80
Identity = 147/264 (55.68%), Postives = 196/264 (74.24%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDVSTLPHHLQAIRESLMVL 77
           ++ ++ +L+E IS+LE L P K VN LFT+LV+ C+ P+ IDVS L   +Q IR  L+ L
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKKVAFV 137
           CG+AEGLLE  FS +L     PL +L +FPY+ NYIKL+ LE+ IL  N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDIVEVK 197
           GSGPLPLTS+++A +H+KTT F NYDID  AN +A  +VA+D ++  RM F T D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVELHDL 257
             L+ YD VFLAALVGM KE+KVK++ HL KYM  G  L++RSA GARAFLYPV++  DL
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFEVLSIFHPTDDVVNSVLLARK 282
            GFEVLS++HPTD+V+NSV++ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.8e-77
Identity = 148/268 (55.22%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPS-SIDVSTLPHHLQAIRESLMV 77
           L+ +I DL+E ISKLETLKPC+ V+TLF  LV+ CI P+ +IDV+ +  ++Q +R +L+ 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKIL--NDNGVVNPKK 137
           +CG AEG LE  FS++L      PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+DID  AN VA ++V+SD +L  RM F T DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVE 257
           ++V E L+G+D VFLAALVGM K+EKVK++ HL K+M  G +L++RSA G RAFLYP+VE
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 LHDLVGFEVLSIFHPTDDVVNSVLLARK 282
             DL GFEVLS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.9e-74
Identity = 151/274 (55.11%), Postives = 201/274 (73.36%), Query Frame = 0


Query: 16  EFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPS-SIDVSTLPHHLQAIRESL 75
           E L+  I DL+E ISKLE+LKP + VN LF  LV+ CI P+ +IDV+ +   +Q IR +L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  MVLCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKIL--NDNGVVNP 135
           + +CG AEG LE  FS++L      PL++L +FPYY NY+KL  LE  +L  N NG V P
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-P 125

Query: 136 KKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTR 195
           K VAF+GSGPLPLTSI++A  H+K T F N+DID  AN +A  +V+SD ++  RM F T 
Sbjct: 126 KSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTV 185

Query: 196 DIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPV 255
           DI++V E L+ +D VFLAALVGM KEEKVK++ HL+K+M  G +L++RSA G RAFLYP+
Sbjct: 186 DIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPI 245

Query: 256 VELHDLVGFEVLSIFHPTDDVVNSVLLARK-PII 285
           VE  DL GFEVLSI+HPTDDV+NSV++++K P++
Sbjct: 246 VEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVV 278

[Close]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.0e-71
Identity = 138/268 (51.49%), Postives = 193/268 (72.01%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCI-LPSSIDVSTLPHHLQAIRESLMV 77
           ++ +IIDL++ ISKL++LKP K V+TLF  LV+ C+   ++IDV+ +   ++ +R +L+ 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGRAEGLLEFEFSTLLGKIPK---PLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKK 137
           LCG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVE 257
           +   E L+ YD VFLAALVGM KE KVK + HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 LHDLVGFEVLSIFHPTDDVVNSVLLARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 8.8e-71
Identity = 139/267 (52.06%), Postives = 190/267 (71.16%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCI-LPSSIDVSTL-PHHLQAIRESLM 77
           ++ +I+DL+  IS LE+LKP K V+TLF  LV+ C+   ++IDV+ +    ++ +R  L+
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKKV 137
            LCG AEG LE  FS +LG     PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V+ D++L  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVEL 257
             KE L+ YD VFLAALVGM KE KVK + HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 HDLVGFEVLSIFHPTDDVVNSVLLARK 282
            DL GFEVL+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lag0033836 vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.9e-133
Identity = 242/285 (84.91%), Postives = 263/285 (92.28%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           M ALQI GTET+IS E LIAR+IDLH SIS+LETLKPCK+VN LFTNLV  CILP SIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP HLQAIRESL++ CGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDIDS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+KKE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           AKG RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI+I
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Lag0033836 vs. ExPASy TrEMBL
Match: A0A6J1E292 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.2e-132
Identity = 240/285 (84.21%), Postives = 264/285 (92.63%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAAL I GTET+IS EFLIAR+IDLH SIS+LETLKPCK+VN LFT+LV LCILP SIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STL  HLQAIRESL++LCGRAEGLLE EFST+LGKIPKPLNNLTLFPYY NYIKLAHLE+
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KIL DNGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+DS AN+VA K+VASD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKFWTRDIVEV EELEGYDCVFLAALVG+ KE+KVKIL HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARKPIII 286
           AKG RAFLYPVVE+ D+VGF++LSIFHP+DDVVNSVLLARKPI++
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Lag0033836 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.1e-131
Identity = 240/281 (85.41%), Postives = 260/281 (92.53%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAALQ HG E EISA FL+ RIIDLH+SI KLETL+PCKKVN LFTNLVNLCILP SIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP +LQAIRESL++LCG+AEGLLE EFSTLL KIPKPLNNLTLFPYYQNY+KLA+LE+
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYD+DS ANDVARK+V SDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEEL GYDCVFLAALVGM KEEKVKI+ HLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARK 282
           A+G RAFLYPVVE+ DLVGFE+LSIFHPTDDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lag0033836 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.1e-131
Identity = 240/281 (85.41%), Postives = 260/281 (92.53%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAALQ HG E EISA FL+ RIIDLH+SI KLETL+PCKKVN LFTNLVNLCILP SIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP +LQAIRESL++LCG+AEGLLE EFSTLL KIPKPLNNLTLFPYYQNY+KLA+LE+
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK TYFDNYD+DS ANDVARK+V SDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           ELEGRMKF + DIVEVKEEL GYDCVFLAALVGM KEEKVKI+ HLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARK 282
           A+G RAFLYPVVE+ DLVGFE+LSIFHPTDDVVNSV+LARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lag0033836 vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.7e-131
Identity = 239/281 (85.05%), Postives = 260/281 (92.53%), Query Frame = 0


Query: 1   MAALQIHGTETEISAEFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPSSIDV 60
           MAALQIHGTE EISA FL+ RIIDLHESISKLETL+PCKKVN LFTNLV LCILP SIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPHHLQAIRESLMVLCGRAEGLLEFEFSTLLGKIPKPLNNLTLFPYYQNYIKLAHLEH 120
           STLP +LQ IRESL++LCG+AEGLLE EFSTLL KIPKPLNNLTLFPYYQNYIKLA+LE+
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGVVNPKKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDA 180
           KILNDNG+VNPKKVAFVGSGPLPLTSIIMAMQHMK T+FDNYD+DS ANDVARK+V SD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFWTRDIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRS 240
           +LEGRMKF + DIV+VKEEL GYDCVFLAALVGM KEEKVKI+ HLRKYMKEGGIL+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGARAFLYPVVELHDLVGFEVLSIFHPTDDVVNSVLLARK 282
           AKG RAFLYPVVE+ DLVGFE+LSIFHPTDDVVNSV+L RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Lag0033836 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 290.0 bits (741), Expect = 2.0e-78
Identity = 148/268 (55.22%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPS-SIDVSTLPHHLQAIRESLMV 77
           L+ +I DL+E ISKLETLKPC+ V+TLF  LV+ CI P+ +IDV+ +  ++Q +R +L+ 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKIL--NDNGVVNPKK 137
           +CG AEG LE  FS++L      PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+DID  AN VA ++V+SD +L  RM F T DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVE 257
           ++V E L+G+D VFLAALVGM K+EKVK++ HL K+M  G +L++RSA G RAFLYP+VE
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 LHDLVGFEVLSIFHPTDDVVNSVLLARK 282
             DL GFEVLS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lag0033836 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 280.0 bits (715), Expect = 2.1e-75
Identity = 151/274 (55.11%), Postives = 201/274 (73.36%), Query Frame = 0


Query: 16  EFLIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCILPS-SIDVSTLPHHLQAIRESL 75
           E L+  I DL+E ISKLE+LKP + VN LF  LV+ CI P+ +IDV+ +   +Q IR +L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  MVLCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKIL--NDNGVVNP 135
           + +CG AEG LE  FS++L      PL++L +FPYY NY+KL  LE  +L  N NG V P
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-P 125

Query: 136 KKVAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTR 195
           K VAF+GSGPLPLTSI++A  H+K T F N+DID  AN +A  +V+SD ++  RM F T 
Sbjct: 126 KSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTV 185

Query: 196 DIVEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPV 255
           DI++V E L+ +D VFLAALVGM KEEKVK++ HL+K+M  G +L++RSA G RAFLYP+
Sbjct: 186 DIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPI 245

Query: 256 VELHDLVGFEVLSIFHPTDDVVNSVLLARK-PII 285
           VE  DL GFEVLSI+HPTDDV+NSV++++K P++
Sbjct: 246 VEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVV 278

[Close]

BLAST of Lag0033836 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 271.6 bits (693), Expect = 7.4e-73
Identity = 138/268 (51.49%), Postives = 193/268 (72.01%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCI-LPSSIDVSTLPHHLQAIRESLMV 77
           ++ +IIDL++ ISKL++LKP K V+TLF  LV+ C+   ++IDV+ +   ++ +R +L+ 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGRAEGLLEFEFSTLLGKIPK---PLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKK 137
           LCG AEG LE  FST+LG + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V+ D +L  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVE 257
           +   E L+ YD VFLAALVGM KE KVK + HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 LHDLVGFEVLSIFHPTDDVVNSVLLARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Lag0033836 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 268.5 bits (685), Expect = 6.3e-72
Identity = 139/267 (52.06%), Postives = 190/267 (71.16%), Query Frame = 0


Query: 18  LIARIIDLHESISKLETLKPCKKVNTLFTNLVNLCI-LPSSIDVSTL-PHHLQAIRESLM 77
           ++ +I+DL+  IS LE+LKP K V+TLF  LV+ C+   ++IDV+ +    ++ +R  L+
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGRAEGLLEFEFSTLLGKI-PKPLNNLTLFPYYQNYIKLAHLEHKILNDNGVVNPKKV 137
            LCG AEG LE  FS +LG     PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKTTYFDNYDIDSKANDVARKMVASDAELEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+DIDS AN +A  +V+ D++L  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELEGYDCVFLAALVGMKKEEKVKILGHLRKYMKEGGILVVRSAKGARAFLYPVVEL 257
             KE L+ YD VFLAALVGM KE KVK + HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 HDLVGFEVLSIFHPTDDVVNSVLLARK 282
            DL GFEVL+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lag0033836 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-08
Identity = 30/54 (55.56%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 230 MKEGGILVVRSAKGARAFLYPVVELHDLVGFEVLSIFHP--TDDVVNSVLLARK 282
           M  G +L++RSA+G R+FLY  V+  DL GFEVL I+HP  +D  VNSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of Lag0033836 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880344.1	2.3e-137	88.07	nicotianamine synthase-like [Benincasa hispida]	[more]
KAG6589715.1	7.8e-133	84.56	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022987937.1	1.0e-132	84.91	nicotianamine synthase-like [Cucurbita maxima]	[more]
KAG7023395.1	8.6e-132	83.86	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022922054.1	8.6e-132	84.21	nicotianamine synthase-like [Cucurbita moschata]	[more]

BLAST of Lag0033836 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	6.1e-80	55.68	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	2.8e-77	55.22	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	2.9e-74	55.11	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	1.0e-71	51.49	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	8.8e-71	52.06	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Lag0033836 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBQ8	4.9e-133	84.91	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]
A0A6J1E292	4.2e-132	84.21	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 S...	[more]
A0A5D3CIT2	2.1e-131	85.41	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	2.1e-131	85.41	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]
A0A0A0LUJ7	2.7e-131	85.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]

BLAST of Lag0033836 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	2.0e-78	55.22	nicotianamine synthase 4	[more]
AT1G09240.1	2.1e-75	55.11	nicotianamine synthase 3	[more]
AT5G04950.1	7.4e-73	51.49	nicotianamine synthase 1	[more]
AT5G56080.1	6.3e-72	52.06	nicotianamine synthase 2	[more]
AT4G26483.1	1.7e-08	55.56	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 15..282 e-value: 2.6E-61 score: 209.1
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 1..283
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 163..238 e-value: 0.00531571 score: 33.9427
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..283 e-value: 5.6E-107 score: 357.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 10..285 score: 48.646309
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 117..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033836.1	Lag0033836.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity