Lag0033824 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033824
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionF-box protein PP2-B10-like
Locationchr3: 2113779 .. 2114258 (-)
RNA-Seq ExpressionLag0033824
SyntenyLag0033824
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGAGATGGAACGGGAAGAAGAAACGAGAACAGGAGCGGAGGACGAAGAAGTTGCCGGTTTACCTTCTCTGCCTGAAGGAGTAATAGCTAACATCTTGTCGCTCACGCCTCCATCTGATGCCTGCAGGTCGTCGGCGGTTTCTCGGACTTTCCATGCGGCGGCGCAGTCCGATATCGTGTGGGACAGATTCCTGCCTAACGATCTGGACGTTTTGATTTCTCGGAGGAATTCCGATCACCTGAATTTTAATCTAATTTCCAATTCGAAGAAGGAGATCTTTTTCTTCCTTTGCGACAACCCATTGCTAATTGACGGCGGCAAGAAGGTAAGGTTTCCGATTCCTCCCAATTTCTCTAAATTGGTGGTGGCCGGAGATGGACGAGTGGTCGGCAGACACAACTCTTACTCCGGCCATACCATCATCCGAAGCCCGCCCTTGTATTGTAATGAAACGAATACTTTCCGAGAACATTAA

mRNA sequence

ATGAACGAGATGGAACGGGAAGAAGAAACGAGAACAGGAGCGGAGGACGAAGAAGTTGCCGGTTTACCTTCTCTGCCTGAAGGAGTAATAGCTAACATCTTGTCGCTCACGCCTCCATCTGATGCCTGCAGGTCGTCGGCGGTTTCTCGGACTTTCCATGCGGCGGCGCAGTCCGATATCGTGTGGGACAGATTCCTGCCTAACGATCTGGACGTTTTGATTTCTCGGAGGAATTCCGATCACCTGAATTTTAATCTAATTTCCAATTCGAAGAAGGAGATCTTTTTCTTCCTTTGCGACAACCCATTGCTAATTGACGGCGGCAAGAAGGTAAGGTTTCCGATTCCTCCCAATTTCTCTAAATTGGTGGTGGCCGGAGATGGACGAGTGGTCGGCAGACACAACTCTTACTCCGGCCATACCATCATCCGAAGCCCGCCCTTGTATTGTAATGAAACGAATACTTTCCGAGAACATTAA

Coding sequence (CDS)

ATGAACGAGATGGAACGGGAAGAAGAAACGAGAACAGGAGCGGAGGACGAAGAAGTTGCCGGTTTACCTTCTCTGCCTGAAGGAGTAATAGCTAACATCTTGTCGCTCACGCCTCCATCTGATGCCTGCAGGTCGTCGGCGGTTTCTCGGACTTTCCATGCGGCGGCGCAGTCCGATATCGTGTGGGACAGATTCCTGCCTAACGATCTGGACGTTTTGATTTCTCGGAGGAATTCCGATCACCTGAATTTTAATCTAATTTCCAATTCGAAGAAGGAGATCTTTTTCTTCCTTTGCGACAACCCATTGCTAATTGACGGCGGCAAGAAGGTAAGGTTTCCGATTCCTCCCAATTTCTCTAAATTGGTGGTGGCCGGAGATGGACGAGTGGTCGGCAGACACAACTCTTACTCCGGCCATACCATCATCCGAAGCCCGCCCTTGTATTGTAATGAAACGAATACTTTCCGAGAACATTAA

Protein sequence

MNEMEREEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFLPNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKKVRFPIPPNFSKLVVAGDGRVVGRHNSYSGHTIIRSPPLYCNETNTFREH
Homology
BLAST of Lag0033824 vs. NCBI nr
Match: XP_022134921.1 (F-box protein PP2-B10-like isoform X1 [Momordica charantia])

HSP 1 Score: 152.1 bits (383), Expect = 3.9e-33
Identity = 80/111 (72.07%), Postives = 85/111 (76.58%), Query Frame = 0

Query: 5   EREEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDR 64
           E EEET TG E EE+A   SLPEGVIANILSLT PSDACRSS VSR FHAAAQSDIVWDR
Sbjct: 20  EMEEETMTGTEGEEIADFSSLPEGVIANILSLTGPSDACRSSTVSRAFHAAAQSDIVWDR 79

Query: 65  FLPNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKKVRFPI 116
           FLP D   LI RR    LN + IS+SKKEIFF LCDNPLL D GKK+ F +
Sbjct: 80  FLPTDWYDLICRRKPSDLNIDPISSSKKEIFFSLCDNPLLTDDGKKLSFSL 130

BLAST of Lag0033824 vs. NCBI nr
Match: XP_022134922.1 (F-box protein PP2-B10-like isoform X2 [Momordica charantia])

HSP 1 Score: 151.4 bits (381), Expect = 6.7e-33
Identity = 79/109 (72.48%), Postives = 84/109 (77.06%), Query Frame = 0

Query: 7   EEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFL 66
           EEET TG E EE+A   SLPEGVIANILSLT PSDACRSS VSR FHAAAQSDIVWDRFL
Sbjct: 2   EEETMTGTEGEEIADFSSLPEGVIANILSLTGPSDACRSSTVSRAFHAAAQSDIVWDRFL 61

Query: 67  PNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKKVRFPI 116
           P D   LI RR    LN + IS+SKKEIFF LCDNPLL D GKK+ F +
Sbjct: 62  PTDWYDLICRRKPSDLNIDPISSSKKEIFFSLCDNPLLTDDGKKLSFSL 110

BLAST of Lag0033824 vs. NCBI nr
Match: XP_022929428.1 (F-box protein PP2-B10-like [Cucurbita moschata])

HSP 1 Score: 143.3 bits (360), Expect = 1.8e-30
Identity = 74/102 (72.55%), Postives = 83/102 (81.37%), Query Frame = 0

Query: 7   EEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFL 66
           ++ETRTG ED E+A   SLPEG IANILSLT PSDACRSSAVSRTFHAAA SDIVW+RFL
Sbjct: 3   KDETRTGTEDGEIADFSSLPEGAIANILSLTTPSDACRSSAVSRTFHAAALSDIVWERFL 62

Query: 67  PNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGG 109
           P D + LISRR  + LNF+ I+ S KEIFF LC+NPLLID G
Sbjct: 63  PTDWEDLISRRKPNDLNFDPITWSTKEIFFSLCNNPLLIDDG 104

BLAST of Lag0033824 vs. NCBI nr
Match: XP_038878468.1 (F-box protein PP2-B10-like [Benincasa hispida])

HSP 1 Score: 142.5 bits (358), Expect = 3.1e-30
Identity = 72/104 (69.23%), Postives = 82/104 (78.85%), Query Frame = 0

Query: 7   EEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFL 66
           EEE   G E EE++   SLPEGV+A ILSLT PSDACRSSAVSRTFHAAAQSDIVWD+ L
Sbjct: 3   EEEKEKGTEGEEISDFSSLPEGVVAKILSLTTPSDACRSSAVSRTFHAAAQSDIVWDKLL 62

Query: 67  PNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKK 111
           P D  +LISRR   +LNF+ IS+ KKEIFF LC++PLLID G K
Sbjct: 63  PTDWKLLISRRKPSNLNFDPISSPKKEIFFSLCESPLLIDDGNK 106

BLAST of Lag0033824 vs. NCBI nr
Match: KAG7021736.1 (F-box protein PP2-B10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 141.7 bits (356), Expect = 5.3e-30
Identity = 74/106 (69.81%), Postives = 84/106 (79.25%), Query Frame = 0

Query: 6   REEETRTGAEDEEVAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRF 65
           +++ETRTG ED E+A   SLPEG IANILSLT PSDACRSSAVS TFHAAAQSDIVW+RF
Sbjct: 256 QKDETRTGTEDGEIADFSSLPEGAIANILSLTTPSDACRSSAVSHTFHAAAQSDIVWERF 315

Query: 66  LPNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKKV 112
           LP D + LISRR  + LNF+  + S KEIFF LC+NPLLID G  V
Sbjct: 316 LPIDWEDLISRRKPNDLNFDPTTWSTKEIFFSLCNNPLLIDDGYTV 361

BLAST of Lag0033824 vs. ExPASy Swiss-Prot
Match: Q9FV02 (F-box protein SKIP3 OS=Arabidopsis thaliana OX=3702 GN=SKIP3 PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 7.0e-17
Identity = 47/103 (45.63%), Postives = 71/103 (68.93%), Query Frame = 0

Query: 8   EETRTGAEDEEVAG-LPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFL 67
           E+ R+ +  + V+  L SLPEG I+NI+S T P DAC ++AVS+ F +A +SDIVW++FL
Sbjct: 9   EKNRSESRKKIVSSTLDSLPEGCISNIISFTSPEDACVAAAVSKIFESAVKSDIVWEKFL 68

Query: 68  PNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGK 110
           P D + LI+         + + +SKKE++F LC++PLLI+ GK
Sbjct: 69  PTDYESLITP--------SRVFSSKKELYFSLCNDPLLIEDGK 103

BLAST of Lag0033824 vs. ExPASy Swiss-Prot
Match: Q3E6P4 (F-box protein At2g02240 OS=Arabidopsis thaliana OX=3702 GN=At2g02240 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.5e-16
Identity = 42/86 (48.84%), Postives = 59/86 (68.60%), Query Frame = 0

Query: 25  LPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFLPNDLDVLISRRNSDHLNF 84
           LPE  I+NI+S T P DAC +++VS+TF +A  SD VWD+FLP + + L+SR        
Sbjct: 64  LPEDCISNIISFTSPRDACVAASVSKTFESAVSSDCVWDKFLPPEYESLVSR-------- 123

Query: 85  NLISNSKKEIFFFLCDNPLLIDGGKK 111
           + +  SKKE++F LC NP+LI+ GKK
Sbjct: 124 SRVFASKKELYFALCHNPVLIEDGKK 141

BLAST of Lag0033824 vs. ExPASy Swiss-Prot
Match: Q9ZVR5 (Putative F-box protein PP2-B2 OS=Arabidopsis thaliana OX=3702 GN=PP2B2 PE=4 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 1.7e-15
Identity = 41/87 (47.13%), Postives = 60/87 (68.97%), Query Frame = 0

Query: 24  SLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDIVWDRFLPNDLDVLISRRNSDHLN 83
           +LPE  I+NI+S T P DAC +++VS+TF +A  SD VWD+FLP+D   L+         
Sbjct: 49  NLPEDCISNIISFTSPRDACVAASVSKTFESAVNSDSVWDKFLPSDYSSLVPP------- 108

Query: 84  FNLISNSKKEIFFFLCDNPLLI-DGGK 110
            + + +SKKE++F +CDNP+L+ DGGK
Sbjct: 109 -SRVFSSKKELYFAICDNPVLVEDGGK 127

BLAST of Lag0033824 vs. ExPASy Swiss-Prot
Match: Q9ZVQ8 (Putative F-box protein PP2-B8 OS=Arabidopsis thaliana OX=3702 GN=PP2B8 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-14
Identity = 48/110 (43.64%), Postives = 72/110 (65.45%), Query Frame = 0

Query: 4   MEREEET-RTGAEDEE--VAGLPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAAQSDI 63
           ++R ++T R  A D+   VA L  LPE  ++ I+S T P DAC  ++VS+TF +A +SDI
Sbjct: 15  VQRVKKTLRLSASDKSHGVAELDDLPEECVSIIVSFTSPQDACVLASVSKTFASAVKSDI 74

Query: 64  VWDRFLPNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKK 111
           VW++F+P + + LIS+  +    F  +  SKKE++F LCD  +LID GKK
Sbjct: 75  VWEKFIPPEYESLISQSRA----FKFL--SKKELYFALCDKSVLIDDGKK 118

BLAST of Lag0033824 vs. ExPASy Swiss-Prot
Match: Q9ZVQ9 (F-box protein PP2-B7 OS=Arabidopsis thaliana OX=3702 GN=PP2B7 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.2e-14
Identity = 49/114 (42.98%), Postives = 70/114 (61.40%), Query Frame = 0

Query: 4   MEREEET-RTGAEDEEVAG------LPSLPEGVIANILSLTPPSDACRSSAVSRTFHAAA 63
           ++R ++T R  A D++  G      L  LPE  I+ I+S T P DAC  + VS+TF +A 
Sbjct: 15  LQRVKKTLRLSASDQQSQGVTEPLSLGDLPEECISLIISFTSPRDACVFALVSKTFESAV 74

Query: 64  QSDIVWDRFLPNDLDVLISRRNSDHLNFNLISNSKKEIFFFLCDNPLLIDGGKK 111
           QSDIVW++F+P + + L+SR  S H       +SKKE+FF LCD  +LI+  KK
Sbjct: 75  QSDIVWEKFIPPEYESLLSR--SQHF------SSKKELFFALCDESVLINVSKK 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134921.13.9e-3372.07F-box protein PP2-B10-like isoform X1 [Momordica charantia][more]
XP_022134922.16.7e-3372.48F-box protein PP2-B10-like isoform X2 [Momordica charantia][more]
XP_022929428.11.8e-3072.55F-box protein PP2-B10-like [Cucurbita moschata][more]
XP_038878468.13.1e-3069.23F-box protein PP2-B10-like [Benincasa hispida][more]
KAG7021736.15.3e-3069.81F-box protein PP2-B10 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q9FV027.0e-1745.63F-box protein SKIP3 OS=Arabidopsis thaliana OX=3702 GN=SKIP3 PE=1 SV=2[more]
Q3E6P43.5e-1648.84F-box protein At2g02240 OS=Arabidopsis thaliana OX=3702 GN=At2g02240 PE=2 SV=1[more]
Q9ZVR51.7e-1547.13Putative F-box protein PP2-B2 OS=Arabidopsis thaliana OX=3702 GN=PP2B2 PE=4 SV=2[more]
Q9ZVQ81.9e-1443.64Putative F-box protein PP2-B8 OS=Arabidopsis thaliana OX=3702 GN=PP2B8 PE=4 SV=1[more]
Q9ZVQ94.2e-1442.98F-box protein PP2-B7 OS=Arabidopsis thaliana OX=3702 GN=PP2B7 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 25..65
e-value: 8.4E-4
score: 28.6
IPR001810F-box domainPFAMPF12937F-box-likecoord: 22..64
e-value: 1.2E-6
score: 28.3
IPR001810F-box domainPROSITEPS50181FBOXcoord: 19..65
score: 9.788908
NoneNo IPR availableGENE3D1.20.1280.50coord: 11..96
e-value: 7.8E-7
score: 30.9
NoneNo IPR availablePANTHERPTHR32278:SF71SUBFAMILY NOT NAMEDcoord: 15..110
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 15..110
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 10..96

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033824.1Lag0033824.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding