Lag0033814 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033814 vs. NCBI nr
Match: XP_038879828.1 (putative lipase ROG1 isoform X1 [Benincasa hispida])

HSP 1 Score: 530.0 bits (1364), Expect = 1.4e-146
Identity = 274/320 (85.62%), Postives = 289/320 (90.31%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTP-HCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFAR 62
           AANTPL+TPPSGPPLD SST  HCPSN    F L FPP PFPD  P LSLS+H RMDFA 
Sbjct: 5   AANTPLYTPPSGPPLDHSSTTRHCPSN---SFTLSFPPTPFPD--PSLSLSNHSRMDFAG 64

Query: 63  RLAATLFCTRRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           RLA + F TR  K LKV+ADFAA++FF  DA APPEHLVIMVNGLIGSAADWRYAA QFV
Sbjct: 65  RLATSCFRTR--KGLKVEADFAAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAAQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
           K+LPDKV+VHRSECNTSR TFDGVDTMGERLAEEVL VIRRRPE+QKISFVAHSLGGLV+
Sbjct: 125 KKLPDKVIVHRSECNTSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVS 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIP+LESSGAP+SF  EE KQHIEQFHRARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPQLESSGAPQSFSAEEKKQHIEQFHRARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 317

BLAST of Lag0033814 vs. NCBI nr
Match: XP_038879829.1 (putative lipase ROG1 isoform X2 [Benincasa hispida])

HSP 1 Score: 530.0 bits (1364), Expect = 1.4e-146
Identity = 274/320 (85.62%), Postives = 289/320 (90.31%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTP-HCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFAR 62
           AANTPL+TPPSGPPLD SST  HCPSN    F L FPP PFPD  P LSLS+H RMDFA 
Sbjct: 5   AANTPLYTPPSGPPLDHSSTTRHCPSN---SFTLSFPPTPFPD--PSLSLSNHSRMDFAG 64

Query: 63  RLAATLFCTRRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           RLA + F TR  K LKV+ADFAA++FF  DA APPEHLVIMVNGLIGSAADWRYAA QFV
Sbjct: 65  RLATSCFRTR--KGLKVEADFAAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAAQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
           K+LPDKV+VHRSECNTSR TFDGVDTMGERLAEEVL VIRRRPE+QKISFVAHSLGGLV+
Sbjct: 125 KKLPDKVIVHRSECNTSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVS 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIP+LESSGAP+SF  EE KQHIEQFHRARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPQLESSGAPQSFSAEEKKQHIEQFHRARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 317

BLAST of Lag0033814 vs. NCBI nr
Match: KAG6589725.1 (hypothetical protein SDJN03_15148, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023402.1 hypothetical protein SDJN02_14427 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 529.3 bits (1362), Expect = 2.4e-146
Identity = 270/320 (84.38%), Postives = 285/320 (89.06%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTPL TPPSGP  DPS+T HC SNPFP F +RFPP PFPD  P LSLS+H RMD   R
Sbjct: 5   AANTPLQTPPSGPLFDPSATRHCLSNPFPRFTIRFPPKPFPD--PALSLSNHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  DA A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERLAEEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 TKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDHIL
Sbjct: 305 ALRAFKRRVAYANVNYDHIL 322

BLAST of Lag0033814 vs. NCBI nr
Match: XP_023516858.1 (putative lipase YOR059C isoform X3 [Cucurbita pepo subsp. pepo] >XP_023516859.1 putative lipase YOR059C isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 3.4e-145
Identity = 268/320 (83.75%), Postives = 284/320 (88.75%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTPL TPPSGP  DPS+T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPLQTPPSGPLFDPSATRHCLSNPFPRFTIRFPPKPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F   A A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFVPVAKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERLAEEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 MKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. NCBI nr
Match: XP_023516856.1 (uncharacterized protein LOC111780634 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 3.4e-145
Identity = 268/320 (83.75%), Postives = 284/320 (88.75%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTPL TPPSGP  DPS+T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPLQTPPSGPLFDPSATRHCLSNPFPRFTIRFPPKPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F   A A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFVPVAKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERLAEEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 MKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. ExPASy Swiss-Prot
Match: P53118 (Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROG1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.7e-15
Identity = 71/228 (31.14%), Postives = 103/228 (45.18%), Query Frame = 0

Query: 97  EHLVIMVNGLIGS-AADWRYAAEQFV---KRLPDKVVVHRSECNTSRLTFDGVDTMGERL 156
           +HLV++ +GL  + + D  Y  EQ     K  P + +V +        T  GV  +G RL
Sbjct: 188 KHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLGTRL 247

Query: 157 AEEVLAVIRRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQHI 216
           AE ++  +     ++KISFV HSLGGL+  +AI  +++  P          FF +     
Sbjct: 248 AEYIIQDLYDE-SIRKISFVGHSLGGLIQAFAIAYIYEVYP---------WFFKK----- 307

Query: 217 EQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGKHL 276
                     + P+NFIT+A+P LG   +    +   L F           V G++G+ L
Sbjct: 308 ----------VNPINFITLASPLLGIVTDNPAYIKVLLSF----------GVIGKTGQDL 367

Query: 277 FLTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
            L +D   GKP L L        L  I  LR FKRR  YAN   D I+
Sbjct: 368 GLENDVEVGKPLLYL-----LSGLPLIEILRRFKRRTVYANAINDGIV 375

BLAST of Lag0033814 vs. ExPASy Swiss-Prot
Match: Q08448 (Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LPL1 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 8.5e-14
Identity = 62/231 (26.84%), Postives = 108/231 (46.75%), Query Frame = 0

Query: 97  EHLVIMVNGLIGS---AADWRYAAEQFVKR--LPDKVVVHRSECNTSRLTFDGVDTMGER 156
           +HL ++++GL G+       R      +K+  + D ++    + N    TFDG++ +G R
Sbjct: 5   KHLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYFLPKQNAMFKTFDGIEIIGYR 64

Query: 157 LAEEVLAVIR--RRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEK 216
              EV   IR  +  ++ K+S + +S GGLVAR+ IG++     +L              
Sbjct: 65  TLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKMLTEFKEL-------------- 124

Query: 217 QHIEQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHL---VAG 276
                        +EP  FIT+ATPHLG     +F    G+ +     S    L   + G
Sbjct: 125 ----------FEDIEPQLFITMATPHLG----VEFYNPTGIAYKSALYSALRTLGSTILG 184

Query: 277 RSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYD 318
           +SG+ +F+ +  N+    +L+++       +++ AL  FK R+A+ANV  D
Sbjct: 185 KSGREMFIANSSNN----ILVKL----SQGEYLEALSLFKWRIAFANVKND 199

BLAST of Lag0033814 vs. ExPASy Swiss-Prot
Match: O14162 (Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.10 PE=3 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-12
Identity = 63/230 (27.39%), Postives = 104/230 (45.22%), Query Frame = 0

Query: 98  HLVIMVNGLIGS-AADWRYAAEQFVK--RLPDKVVVHRSECNTSRLTFDGVDTMGERLAE 157
           HLV++ +G+  +  AD  Y  E+ ++  +   ++VV R        T  GV  +G+RL E
Sbjct: 306 HLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQTEKGVRWLGKRLGE 365

Query: 158 EVLAVI----RRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQ 217
            +L +        P    IS VAHSLGGLV  YA+G +                      
Sbjct: 366 WLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVH--------------------- 425

Query: 218 HIEQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGK 277
              + H A    + PV F+T+ATP LG  G  + P   G           ++ + G++G+
Sbjct: 426 --AKTHGAFFQAIHPVFFVTLATPWLGVAG--EHPSYIG--------KALSYGIIGKTGQ 485

Query: 278 HLFLTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
            L LT  ++  +    L +++D     F  A+  F++R+ +AN   D+I+
Sbjct: 486 DLSLTPLNHSIESRPFLVLMSDP-STPFFQAVSFFEKRILFANTTNDYIV 501

BLAST of Lag0033814 vs. ExPASy Swiss-Prot
Match: Q12103 (Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDL109C PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-10
Identity = 66/227 (29.07%), Postives = 99/227 (43.61%), Query Frame = 0

Query: 98  HLVIMVNGLIGS-AADWRYAAEQFVK---RLPDKVVVHRSECNTSRLTFDGVDTMGERLA 157
           HLVI+ +G   + +AD  Y  E+  K     P++ +V +     +  T  G+  +G  LA
Sbjct: 194 HLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKFLGVGLA 253

Query: 158 EEVLAVIRRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQHIE 217
             ++  +     V KISF+ HSLGGL   +AI  +    P          FF +      
Sbjct: 254 NYIIDEL-YDDSVGKISFIGHSLGGLTQTFAICYIKTKYP---------YFFKK------ 313

Query: 218 QFHRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGKHLF 277
                    +EP+NFI++A+P LG        +    P   + +      + G +G+ L 
Sbjct: 314 ---------VEPINFISLASPLLG--------IATSTPNYVKMSLSMG--IIGTTGQELG 373

Query: 278 LTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
           L  D N G  PLL  +  +S     IS L  FKRR  YAN   D I+
Sbjct: 374 L-KDGNYGDKPLLYLLSEES----LISVLARFKRRTLYANAVNDGIV 380

BLAST of Lag0033814 vs. ExPASy Swiss-Prot
Match: Q9P2D6 (Protein FAM135A OS=Homo sapiens OX=9606 GN=FAM135A PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 2.2e-09
Identity = 54/206 (26.21%), Postives = 87/206 (42.23%), Query Frame = 0

Query: 91   DANAPPEHLVIMVNGLIGSAADWRYAAEQFVKRLPDKVVVHRSECNTSRLTFDGVDTMGE 150
            D +    HL++ V+GL G++AD R         LP   +           TF   D+M +
Sbjct: 1242 DGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRIDFLMSERNQNDTFADFDSMTD 1301

Query: 151  RLAEEVLAVIR-RRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEK 210
            RL +E++  I+     V KISF+ HSLG L+ R  + R                  P  K
Sbjct: 1302 RLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTR------------------PRFK 1361

Query: 211  QHIEQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSG 270
             ++ + H           F++++ PHLG+  N    V  GL F+++           +SG
Sbjct: 1362 YYLNKLH----------TFLSLSGPHLGTLYNSSALVNTGLWFMQKWK---------KSG 1410

Query: 271  KHLFLT-DDDNDGKPPLLLRMVTDSG 295
              L LT  D +D +   L ++   +G
Sbjct: 1422 SLLQLTCRDHSDPRQTFLYKLSNKAG 1410

BLAST of Lag0033814 vs. ExPASy TrEMBL
Match: A0A6J1JJS1 (putative lipase YOR059C isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111485732 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.7e-145
Identity = 266/320 (83.12%), Postives = 282/320 (88.12%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTP  TPPSGP  DPS T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPFQTPPSGPLFDPSPTRHCLSNPFPRFTIRFPPRPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  D  A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFGPDVKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERL+EEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 LKLPDNVIVHRSECNTSRLTFDGVDTMGERLSEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. ExPASy TrEMBL
Match: A0A6J1JMH9 (uncharacterized protein LOC111485732 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485732 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.7e-145
Identity = 266/320 (83.12%), Postives = 282/320 (88.12%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTP  TPPSGP  DPS T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPFQTPPSGPLFDPSPTRHCLSNPFPRFTIRFPPRPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  D  A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFGPDVKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERL+EEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 LKLPDNVIVHRSECNTSRLTFDGVDTMGERLSEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. ExPASy TrEMBL
Match: A0A6J1JLT6 (putative lipase YOR059C isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485732 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.7e-145
Identity = 266/320 (83.12%), Postives = 282/320 (88.12%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTP  TPPSGP  DPS T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPFQTPPSGPLFDPSPTRHCLSNPFPRFTIRFPPRPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  D  A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFGPDVKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERL+EEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 LKLPDNVIVHRSECNTSRLTFDGVDTMGERLSEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. ExPASy TrEMBL
Match: A0A6J1JD84 (putative lipase YOR059C isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111485732 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 4.8e-145
Identity = 267/320 (83.44%), Postives = 281/320 (87.81%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTP  TPPSGP  DPS T HC SNPFP F +RFPP PFPD  P LSLSDH RMD   R
Sbjct: 5   AANTPFQTPPSGPLFDPSPTRHCLSNPFPRFTIRFPPRPFPD--PALSLSDHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  D  A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFGPDVKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERL+EEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 LKLPDNVIVHRSECNTSRLTFDGVDTMGERLSEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAFKRRVAYANVNYDH L
Sbjct: 305 ALRAFKRRVAYANVNYDHRL 322

BLAST of Lag0033814 vs. ExPASy TrEMBL
Match: A0A6J1E5F9 (putative lipase YOR059C isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.4e-144
Identity = 266/320 (83.12%), Postives = 284/320 (88.75%), Query Frame = 0

Query: 3   AANTPLHTPPSGPPLDPSSTPHCPSNPFPPFNLRFPPNPFPDPPPPLSLSDHPRMDFARR 62
           AANTPL TPPSGP  DPS+T HC SNP P F +RFPP PFPD  P LSLS+H RMD   R
Sbjct: 5   AANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPD--PALSLSNHSRMDLIGR 64

Query: 63  LAATLFCT-RRTKALKVQADFAADDFFDYDANAPPEHLVIMVNGLIGSAADWRYAAEQFV 122
           LA + FC+ RR K LKV+ADF+ ++ F  DA A PEHLVIMVNGLIGSAADWRYAAEQFV
Sbjct: 65  LANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQFV 124

Query: 123 KRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVA 182
            +LPD V+VHRSECNTSRLTFDGVDTMGERLAEEVLAVIRR PEVQKISFVAHSLGGLVA
Sbjct: 125 MKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVA 184

Query: 183 RYAIGRLFDHIPKLESSGAPESFFPEE-KQHIEQFHRARIAGLEPVNFITVATPHLGSRG 242
           RYAIGRLFDHIPKLESSGAP+SF  EE KQ +EQFH ARIAGLEPVNFITVATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFIS 302
           NKQFPVLCGLPFLERRASQTAHL+AGRSGKHLFLTDD+NDGKPPLLLRMVTDS DLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRAFKRRVAYANVNYDHIL 321
           ALRAF+RRVAYANVNYDH++
Sbjct: 305 ALRAFERRVAYANVNYDHMV 322

BLAST of Lag0033814 vs. TAIR 10
Match: AT4G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 9.2e-88
Identity = 179/313 (57.19%), Postives = 219/313 (69.97%), Query Frame = 0

Query: 15  PPLDPSSTPHCP--SNPFP-PFNLRFPPNPFPDPPPPLSLSDHPRMDFARRLAATLFCTR 74
           P L PSS    P   N F     L+FP  P           +  +M++  RL    F   
Sbjct: 13  PDLIPSSPRFLPPSQNQFRFRLYLQFPAQP----------KNLCQMEWMNRLGIGCFAAN 72

Query: 75  RTK-ALKVQADFAADDFFDYD---ANAPPEHLVIMVNGLIGSAADWRYAAEQFVKRLPDK 134
           R+K  L  + D   +DFFD D   +   P+HLV+MVNG++GSAADW+YAAEQFVK+ PDK
Sbjct: 73  RSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADWKYAAEQFVKKFPDK 132

Query: 135 VVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVARYAIGR 194
           V+VHRSE N++ LTFDGVD MGERLA EVL V++ R  ++KISFVAHSLGGLVARYAIG+
Sbjct: 133 VLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVAHSLGGLVARYAIGK 192

Query: 195 LFDHIPKLESSGAPESFFPEEKQHIEQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVL 254
           L++   +++S  +P     +EK          IAGLEP+NFIT ATPHLGSRG++QFP+L
Sbjct: 193 LYEQPGEVDSLDSPS----KEK----SARGGEIAGLEPMNFITFATPHLGSRGHRQFPIL 252

Query: 255 CGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKR 314
           CGLPFLER ASQTAHL AGR+GKHLFL D+D DG  PLL+RM TDS DLKFISAL AFKR
Sbjct: 253 CGLPFLERTASQTAHLAAGRTGKHLFLVDND-DGNAPLLIRMATDSDDLKFISALNAFKR 306

Query: 315 RVAYANVNYDHIL 321
           RVAYANVN+D ++
Sbjct: 313 RVAYANVNFDSMV 306

BLAST of Lag0033814 vs. TAIR 10
Match: AT4G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 9.2e-88
Identity = 179/313 (57.19%), Postives = 219/313 (69.97%), Query Frame = 0

Query: 15  PPLDPSSTPHCP--SNPFP-PFNLRFPPNPFPDPPPPLSLSDHPRMDFARRLAATLFCTR 74
           P L PSS    P   N F     L+FP  P           +  +M++  RL    F   
Sbjct: 13  PDLIPSSPRFLPPSQNQFRFRLYLQFPAQP----------KNLCQMEWMNRLGIGCFAAN 72

Query: 75  RTK-ALKVQADFAADDFFDYD---ANAPPEHLVIMVNGLIGSAADWRYAAEQFVKRLPDK 134
           R+K  L  + D   +DFFD D   +   P+HLV+MVNG++GSAADW+YAAEQFVK+ PDK
Sbjct: 73  RSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADWKYAAEQFVKKFPDK 132

Query: 135 VVVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRRPEVQKISFVAHSLGGLVARYAIGR 194
           V+VHRSE N++ LTFDGVD MGERLA EVL V++ R  ++KISFVAHSLGGLVARYAIG+
Sbjct: 133 VLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVAHSLGGLVARYAIGK 192

Query: 195 LFDHIPKLESSGAPESFFPEEKQHIEQFHRARIAGLEPVNFITVATPHLGSRGNKQFPVL 254
           L++   +++S  +P     +EK          IAGLEP+NFIT ATPHLGSRG++QFP+L
Sbjct: 193 LYEQPGEVDSLDSPS----KEK----SARGGEIAGLEPMNFITFATPHLGSRGHRQFPIL 252

Query: 255 CGLPFLERRASQTAHLVAGRSGKHLFLTDDDNDGKPPLLLRMVTDSGDLKFISALRAFKR 314
           CGLPFLER ASQTAHL AGR+GKHLFL D+D DG  PLL+RM TDS DLKFISAL AFKR
Sbjct: 253 CGLPFLERTASQTAHLAAGRTGKHLFLVDND-DGNAPLLIRMATDSDDLKFISALNAFKR 306

Query: 315 RVAYANVNYDHIL 321
           RVAYANVN+D ++
Sbjct: 313 RVAYANVNFDSMV 306

BLAST of Lag0033814 vs. TAIR 10
Match: AT1G10040.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 136/225 (60.44%), Postives = 170/225 (75.56%), Query Frame = 0

Query: 96  PEHLVIMVNGLIGSAADWRYAAEQFVKRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEE 155
           P HLV+MVNGLIGSA +WR+AA+Q +K+ P  ++VH S+ N S  TFDGVD MGERLAEE
Sbjct: 79  PTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLLVHCSKRNHSTQTFDGVDVMGERLAEE 138

Query: 156 VLAVIRRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQHIEQF 215
           V +VI+R P +QKISFV HSLGGL+ARYAIGRL++   + E     +     +K  IE+ 
Sbjct: 139 VRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLYEQESREELPHNSDDI--GDKCSIEE- 198

Query: 216 HRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLT 275
            +ARIAGLEPV FIT ATPHLGSRG+KQ P+  G   LER A++ +  + G++GKHLFL 
Sbjct: 199 PKARIAGLEPVYFITSATPHLGSRGHKQVPLFSGSYTLERLATRMSGCL-GKTGKHLFLA 258

Query: 276 DDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
           D D  GKPPLLLRMV DS DLKFISAL+ FKRR+AYAN ++DH++
Sbjct: 259 DSDG-GKPPLLLRMVKDSRDLKFISALQCFKRRIAYANTSFDHLV 298

BLAST of Lag0033814 vs. TAIR 10
Match: AT1G10040.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 136/225 (60.44%), Postives = 170/225 (75.56%), Query Frame = 0

Query: 96  PEHLVIMVNGLIGSAADWRYAAEQFVKRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEE 155
           P HLV+MVNGLIGSA +WR+AA+Q +K+ P  ++VH S+ N S  TFDGVD MGERLAEE
Sbjct: 79  PTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLLVHCSKRNHSTQTFDGVDVMGERLAEE 138

Query: 156 VLAVIRRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQHIEQF 215
           V +VI+R P +QKISFV HSLGGL+ARYAIGRL++   + E     +     +K  IE+ 
Sbjct: 139 VRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLYEQESREELPHNSDDI--GDKCSIEE- 198

Query: 216 HRARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLT 275
            +ARIAGLEPV FIT ATPHLGSRG+KQ P+  G   LER A++ +  + G++GKHLFL 
Sbjct: 199 PKARIAGLEPVYFITSATPHLGSRGHKQVPLFSGSYTLERLATRMSGCL-GKTGKHLFLA 258

Query: 276 DDDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
           D D  GKPPLLLRMV DS DLKFISAL+ FKRR+AYAN ++DH++
Sbjct: 259 DSDG-GKPPLLLRMVKDSRDLKFISALQCFKRRIAYANTSFDHLV 298

BLAST of Lag0033814 vs. TAIR 10
Match: AT5G51180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.2e-67
Identity = 126/224 (56.25%), Postives = 158/224 (70.54%), Query Frame = 0

Query: 97  EHLVIMVNGLIGSAADWRYAAEQFVKRLPDKVVVHRSECNTSRLTFDGVDTMGERLAEEV 156
           +HLV+MV+G++GS  DW++ AEQFVK++PDKV VH SE N S LT DGVD MGERLA EV
Sbjct: 34  DHLVVMVHGILGSTDDWKFGAEQFVKKMPDKVFVHCSEKNVSALTLDGVDVMGERLAAEV 93

Query: 157 LAVIRRRPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPESFFPEEKQHIEQFH 216
           L +I+R+P + KISFVAHSLGGL ARYAIG+L+            E          ++  
Sbjct: 94  LDIIQRKPNICKISFVAHSLGGLAARYAIGKLY-------KPANQEDVKDSVADSSQETP 153

Query: 217 RARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTD 276
           +  I GLE +NFITVATPHLGS GNKQ P L G   +E+ A    H +  R+G+HLFL D
Sbjct: 154 KGTICGLEAMNFITVATPHLGSMGNKQVPFLFGFSSIEKVAGLIIHWIFKRTGRHLFLKD 213

Query: 277 DDNDGKPPLLLRMVTDSGDLKFISALRAFKRRVAYANVNYDHIL 321
           ++ +GKPPLL RMV D+ D  FISALRAFKRRVAY+NV +DH++
Sbjct: 214 EE-EGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNVGHDHVV 249