Lag0033801 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0033801 vs. NCBI nr
Match: XP_023516208.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 440/482 (91.29%), Postives = 461/482 (95.64%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASVPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEAV +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Lag0033801 vs. NCBI nr
Match: XP_022921757.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 910.2 bits (2351), Expect = 7.5e-261
Identity = 438/482 (90.87%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEAV +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Lag0033801 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909.4 bits (2349), Expect = 1.3e-260
Identity = 439/482 (91.08%), Postives = 460/482 (95.44%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASVPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEA  +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Lag0033801 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 907.1 bits (2343), Expect = 6.3e-260
Identity = 438/482 (90.87%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ PP+N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEA  +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Lag0033801 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 903.3 bits (2333), Expect = 9.1e-259
Identity = 437/482 (90.66%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEA  +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Lag0033801 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 6.1e-189
Identity = 315/466 (67.60%), Postives = 379/466 (81.33%), Query Frame = 0

Query: 22  SLKGCG---LSGIRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASATAPPENVVPP- 81
           +++GCG   L+ + IDK EL+ +L MP+YLR+A+RD I+ KD    +     P       
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADR 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL +VKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+KIR+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSMKLT-EESDLNE 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW + + MP+GE V+PP+S+K T EE+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 AVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYS 321
           A   LD  GD+P K  SYEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS
Sbjct: 270 A---LDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYS 329

Query: 322 SYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYAS 381
           SYSCTQGWF T C  +P LRGL+NI++IH+KK NCSEWE+I +P SVRSIV LNL+NY S
Sbjct: 330 SYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGS 389

Query: 382 GRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRI 441
           GR+PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR 
Sbjct: 390 GRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRF 449

Query: 442 EIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           E+RGG  K A+LQMDGEPWKQP+  D STFVEIK+V  QSLMING+
Sbjct: 450 ELRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Lag0033801 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.1e-185
Identity = 309/476 (64.92%), Postives = 378/476 (79.41%), Query Frame = 0

Query: 13  IAARSSLFDS--LKGCGLSG---IRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASA 72
           +A+R S  DS  ++GCGL+    + +DK EL+ +LMMP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSAIPDH 75

Query: 73  TAPPENVVPP-PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQY 132
              P   V    P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFDL +VKPHEFV+Y
Sbjct: 76  LLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRY 135

Query: 133 GLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPL 192
           GL CLE +A  GD CAK+ R ++R+MVAGGDGTVGWVLG L EL K  +  + PVG+IPL
Sbjct: 136 GLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPL 195

Query: 193 GTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSM 252
           GTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW + + MP+GE V+PP+S+
Sbjct: 196 GTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSL 255

Query: 253 KLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 312
           K  EE++L++    LD   D P    +YEGVFYNY SIGMDAQVAYGFHHLRN +PYLAQ
Sbjct: 256 KPAEENELDQG---LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQ 315

Query: 313 GPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSI 372
           GP++NK+IYSS+ C+QGWF T C  DP LRGL+NI++IH+KK+NCS+WE+I +P +VRSI
Sbjct: 316 GPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSI 375

Query: 373 VALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 432
           VALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISAK
Sbjct: 376 VALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAK 435

Query: 433 HIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           HIAQAAA+R E+RGG  + A+LQMDGEPWKQP+S + STFVEIK+V  QSLMIN +
Sbjct: 436 HIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of Lag0033801 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 4.5e-184
Identity = 298/456 (65.35%), Postives = 373/456 (81.80%), Query Frame = 0

Query: 28  LSGIRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASAT-APPENVVPPPPEAPLVVF 87
           L+ IR+ K EL+ ++M+PQYLR+A+RD I  KD +  AS++ APP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL +VKP+EF++YGL CLE  A RGD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           +K+R++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 KSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSMKLTEESDLNEAVHELDERGD 267
           KSA+KR+LHRA+   +  LDSW++ + MP+GE V+PP+S+K T+E  +++    L+  G+
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQ---NLEIEGE 274

Query: 268 LPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFF 327
           +P   + YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYS Y C+QGWF 
Sbjct: 275 IPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFL 334

Query: 328 TSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKP 387
           T C  DP LRGLKNI+ +H+KK++ SEWE++P+P SVR++VALNLH+Y SGRNPWG  K 
Sbjct: 335 THCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQ 394

Query: 388 EYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKA 447
           +YLEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A
Sbjct: 395 DYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDA 454

Query: 448 YLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           ++QMDGEPWKQP+++D STFV+IKRV  QSL++ GD
Sbjct: 455 FMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of Lag0033801 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-72
Identity = 151/404 (37.38%), Postives = 223/404 (55.20%), Query Frame = 0

Query: 74  VVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRC-LE 133
           +V   P  P+VVFIN RSGG+ G  L +  ++L+++ QVFDL +  P E V + L C  E
Sbjct: 37  LVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCNFE 96

Query: 134 ILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDL 193
            L   GD  A Q +  +R++VAGGDGT  W+LG +++L K    P  P+  +PLGTGN+L
Sbjct: 97  KLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-KLSHPP--PIATVPLGTGNNL 156

Query: 194 SRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEK--------VEPPH 253
             SFGWG   P   + AVK  L +    +  ++DSWH+ + M A ++        +E PH
Sbjct: 157 PFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQEGPCEPIAPLELPH 216

Query: 254 SMKLTEESDLNEAVHELDERGDLPTK-LDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPY 313
           S+          A H +     L  +   +Y G F+NYFS+GMDAQV+Y FH  R + P 
Sbjct: 217 SL---------HAFHRVSGSDSLNMEGYHTYRGGFWNYFSMGMDAQVSYEFHSERKRNPE 276

Query: 314 LAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSV 373
             +  +TN+  Y+     QGWF  S +  P+ R +  + ++ + K    +WE++ IP S+
Sbjct: 277 KFKNQLTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQLAKVRIMKRPGGQWEELKIPRSI 336

Query: 374 RSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDII 433
           RSIV LNL +++ G NPWG P    ++ R       DDGL+E+ G +  WH   ++    
Sbjct: 337 RSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAPNG 396

Query: 434 SAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFV 468
               +AQA  IR E   G  +  ++++DGEPWKQP+ KD  T V
Sbjct: 397 HGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDDDTVV 426

BLAST of Lag0033801 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.4e-71
Identity = 158/438 (36.07%), Postives = 236/438 (53.88%), Query Frame = 0

Query: 41  KLMMPQYLRLALRDSIRFKDPAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLK 100
           K  +P Y+ L+  D     +P A  S T  PEN        P++VFIN +SGG+ G +L 
Sbjct: 20  KFFIPSYV-LSPED-----EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELI 79

Query: 101 QRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTV 160
              + L++++QVFDL    P + +Q     LE L D  D+ A + R K++++VAGGDGT 
Sbjct: 80  LTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTA 139

Query: 161 GWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTG 220
           GW+LG +++L   +     P+  +PLGTGN+L  +FGWG   P   +S+V+  L +    
Sbjct: 140 GWLLGVVSDLNLSNPP---PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINA 199

Query: 221 QVRSLDSWHVSLLMPAGEK------VEPPHSMKLTEESDLN--EAVHELDERGDLPTKLD 280
           +   +D+W + + M   ++      ++ PHS+     SD    E  H             
Sbjct: 200 KEMKIDNWKILMRMKHPKEGSCDITLKLPHSLPRIFPSDQENMEGYH------------- 259

Query: 281 SYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKD 340
           +Y G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y   SCTQGWFF S    
Sbjct: 260 TYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-H 319

Query: 341 PNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKR 400
           P+ + +  + +I +   N  +W  + IP S+RSIV LNL +++ G NPWG P P+    R
Sbjct: 320 PSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDR 379

Query: 401 GFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDG 460
                  DDGL+EI G +  WH   ++        +AQA  +R+E + G  K AY+++DG
Sbjct: 380 SLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDG 423

Query: 461 EPWKQPI-SKDLSTFVEI 470
           EPWKQP+ S D +  VEI
Sbjct: 440 EPWKQPLPSNDETVMVEI 423

BLAST of Lag0033801 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.6e-261
Identity = 438/482 (90.87%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEAV +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Lag0033801 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.4e-259
Identity = 437/482 (90.66%), Postives = 458/482 (95.02%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLALRDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P  GASA+ P +N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTTGASASVPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAELYKQDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGEKV
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEA  +LDE+GDLPTKL SYEGVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RPYLAQGP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPYLAQGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Lag0033801 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.7e-258
Identity = 433/482 (89.83%), Postives = 457/482 (94.81%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLA+RDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEAV +LDE+GDLPTKL SY GVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RP LA+GP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPKLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 482

BLAST of Lag0033801 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.6e-256
Identity = 432/482 (89.63%), Postives = 456/482 (94.61%), Query Frame = 0

Query: 1   MDHSRGGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIRFKD 60
           MDHSR G D V+IAARSSLFDSLKGCGLSGI IDK EL TK+MMPQYLRLA+RDSIR KD
Sbjct: 1   MDHSRDGGDQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKD 60

Query: 61  PAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKP 120
           P AGASA+ PP+N+VPP PEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLL+VKP
Sbjct: 61  PTAGASASLPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKP 120

Query: 121 HEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLP 180
           HEFVQYGLRCLEILAD+GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL  QDRKPVLP
Sbjct: 121 HEFVQYGLRCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLP 180

Query: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKV 240
           VGIIPLGTGNDLSRSFGWGGSFPFAW+SAVKRSLHRATTGQV SLDSWHV L +PAGE+V
Sbjct: 181 VGIIPLGTGNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERV 240

Query: 241 EPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNK 300
           EPPHS+KLTEESDLNEA  +LDE+GDLPTKL SY GVFYNYFSIGMDAQVAYGFHHLRNK
Sbjct: 241 EPPHSLKLTEESDLNEA--KLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNK 300

Query: 301 RPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIP 360
           RP LA+GP+TNKLIYSSYSCTQGWFFTSCS DPNLRGLKNI+RIH+KKINCSEWE+IPIP
Sbjct: 301 RPKLARGPLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIP 360

Query: 361 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420
           SSVRSIV LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV
Sbjct: 361 SSVRSIVTLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 420

Query: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMIN 480
           DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPI+KD ST+VEIKRV  QSLMIN
Sbjct: 421 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMIN 480

Query: 481 GD 483
           GD
Sbjct: 481 GD 480

BLAST of Lag0033801 vs. ExPASy TrEMBL
Match: A0A0A0LZ78 (Diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_1G295150 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.0e-255
Identity = 431/485 (88.87%), Postives = 452/485 (93.20%), Query Frame = 0

Query: 1   MDHSR---GGADDVTIAARSSLFDSLKGCGLSGIRIDKHELKTKLMMPQYLRLALRDSIR 60
           MDHS    G  D+VT+AARSS  DSLKGCGLSGIRIDKHEL TK++MPQYLR ALRDSIR
Sbjct: 105 MDHSSAGPGAGDEVTVAARSSFIDSLKGCGLSGIRIDKHELNTKVIMPQYLRFALRDSIR 164

Query: 61  FKDPAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLD 120
            KDP AGASAT PP+N+V P PE PLVVFINPRSGGRHGQKLKQRLQ+LISEEQVFDLLD
Sbjct: 165 LKDPIAGASATTPPQNMVSPLPETPLVVFINPRSGGRHGQKLKQRLQELISEEQVFDLLD 224

Query: 121 VKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKP 180
           VKPHEFVQYGLRCLEILAD GD CAKQTRQKIR+MVAGGDGTVGWVLGSLAELYKQDR P
Sbjct: 225 VKPHEFVQYGLRCLEILADHGDVCAKQTRQKIRIMVAGGDGTVGWVLGSLAELYKQDRNP 284

Query: 181 VLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAG 240
           VLPVGIIPLGTGNDL+RSFGWGGSFPFAWKSAVKRSL RATTGQV SLDSWHVSL MPAG
Sbjct: 285 VLPVGIIPLGTGNDLARSFGWGGSFPFAWKSAVKRSLLRATTGQVGSLDSWHVSLSMPAG 344

Query: 241 EKVEPPHSMKLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHL 300
           EKVEPPHS+KLTEESDLNEAVH+LD+  D PTKL  +EGVFYNYFSIGMDAQVAYGFHHL
Sbjct: 345 EKVEPPHSLKLTEESDLNEAVHKLDDIEDSPTKLTCFEGVFYNYFSIGMDAQVAYGFHHL 404

Query: 301 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQI 360
           RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCS +PNL GLKNI+RIH+KKINC+EWE++
Sbjct: 405 RNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSNNPNLSGLKNIIRIHIKKINCAEWEKV 464

Query: 361 PIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 420
           P+PSSVRSIVALNLHNYASGRNPWGK KPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF
Sbjct: 465 PVPSSVRSIVALNLHNYASGRNPWGKLKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSF 524

Query: 421 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSL 480
           VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISK+ STFVEIKRV  QSL
Sbjct: 525 VMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKENSTFVEIKRVPFQSL 584

Query: 481 MINGD 483
           MINGD
Sbjct: 585 MINGD 589

BLAST of Lag0033801 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 661.8 bits (1706), Expect = 4.3e-190
Identity = 315/466 (67.60%), Postives = 379/466 (81.33%), Query Frame = 0

Query: 22  SLKGCG---LSGIRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASATAPPENVVPP- 81
           +++GCG   L+ + IDK EL+ +L MP+YLR+A+RD I+ KD    +     P       
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 82  PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADR 141
            P AP+VVFINP+SGGRHG  LK+RLQQL++EEQVFDL +VKPHEFV+YGL CL+ LA +
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 142 GDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFG 201
           GD CA++ R+KIR+MVAGGDGTVGWVLG L EL+K  +  + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 202 WGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSMKLT-EESDLNE 261
           WGGSFPFAW+SA+KR+LHRAT G +  LDSW + + MP+GE V+PP+S+K T EE+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 262 AVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYS 321
           A   LD  GD+P K  SYEGVFYNYFSIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS
Sbjct: 270 A---LDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYS 329

Query: 322 SYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYAS 381
           SYSCTQGWF T C  +P LRGL+NI++IH+KK NCSEWE+I +P SVRSIV LNL+NY S
Sbjct: 330 SYSCTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGS 389

Query: 382 GRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRI 441
           GR+PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR 
Sbjct: 390 GRHPWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRF 449

Query: 442 EIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           E+RGG  K A+LQMDGEPWKQP+  D STFVEIK+V  QSLMING+
Sbjct: 450 ELRGGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of Lag0033801 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 651.0 bits (1678), Expect = 7.6e-187
Identity = 309/476 (64.92%), Postives = 378/476 (79.41%), Query Frame = 0

Query: 13  IAARSSLFDS--LKGCGLSG---IRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASA 72
           +A+R S  DS  ++GCGL+    + +DK EL+ +LMMP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSAIPDH 75

Query: 73  TAPPENVVPP-PPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQY 132
              P   V    P AP+VVFINP SGGRHG  LK+RLQQL+SEEQVFDL +VKPHEFV+Y
Sbjct: 76  LLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRY 135

Query: 133 GLRCLEILADRGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPL 192
           GL CLE +A  GD CAK+ R ++R+MVAGGDGTVGWVLG L EL K  +  + PVG+IPL
Sbjct: 136 GLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPL 195

Query: 193 GTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSM 252
           GTGNDLSRSFGWGGSFPFAW+SAVKR+LHRA+ G V  LDSW + + MP+GE V+PP+S+
Sbjct: 196 GTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSL 255

Query: 253 KLTEESDLNEAVHELDERGDLPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 312
           K  EE++L++    LD   D P    +YEGVFYNY SIGMDAQVAYGFHHLRN +PYLAQ
Sbjct: 256 KPAEENELDQG---LDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQ 315

Query: 313 GPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSI 372
           GP++NK+IYSS+ C+QGWF T C  DP LRGL+NI++IH+KK+NCS+WE+I +P +VRSI
Sbjct: 316 GPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSI 375

Query: 373 VALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 432
           VALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISAK
Sbjct: 376 VALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAK 435

Query: 433 HIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           HIAQAAA+R E+RGG  + A+LQMDGEPWKQP+S + STFVEIK+V  QSLMIN +
Sbjct: 436 HIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of Lag0033801 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 645.6 bits (1664), Expect = 3.2e-185
Identity = 298/456 (65.35%), Postives = 373/456 (81.80%), Query Frame = 0

Query: 28  LSGIRIDKHELKTKLMMPQYLRLALRDSIRFKDPAAGASAT-APPENVVPPPPEAPLVVF 87
           L+ IR+ K EL+ ++M+PQYLR+A+RD I  KD +  AS++ APP       PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 88  INPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTR 147
           +NP+SGGR G  +K+RLQ LISEEQV+DL +VKP+EF++YGL CLE  A RGD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 148 QKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 207
           +K+R++VAGGDGTVGWVLG L EL  Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 208 KSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEKVEPPHSMKLTEESDLNEAVHELDERGD 267
           KSA+KR+LHRA+   +  LDSW++ + MP+GE V+PP+S+K T+E  +++    L+  G+
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQ---NLEIEGE 274

Query: 268 LPTKLDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFF 327
           +P   + YEGVFYNYFSIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYS Y C+QGWF 
Sbjct: 275 IPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFL 334

Query: 328 TSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKP 387
           T C  DP LRGLKNI+ +H+KK++ SEWE++P+P SVR++VALNLH+Y SGRNPWG  K 
Sbjct: 335 THCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQ 394

Query: 388 EYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKA 447
           +YLEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A
Sbjct: 395 DYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDA 454

Query: 448 YLQMDGEPWKQPISKDLSTFVEIKRVSCQSLMINGD 483
           ++QMDGEPWKQP+++D STFV+IKRV  QSL++ GD
Sbjct: 455 FMQMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of Lag0033801 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 271.9 bits (694), Expect = 9.6e-73
Identity = 158/438 (36.07%), Postives = 236/438 (53.88%), Query Frame = 0

Query: 41  KLMMPQYLRLALRDSIRFKDPAAGASATAPPENVVPPPPEAPLVVFINPRSGGRHGQKLK 100
           K  +P Y+ L+  D     +P A  S T  PEN        P++VFIN +SGG+ G +L 
Sbjct: 20  KFFIPSYV-LSPED-----EPEAQISCTTAPEN--------PILVFINSKSGGQLGAELI 79

Query: 101 QRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILADRGDACAKQTRQKIRVMVAGGDGTV 160
              + L++++QVFDL    P + +Q     LE L D  D+ A + R K++++VAGGDGT 
Sbjct: 80  LTYRTLLNDKQVFDLEVETPDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTA 139

Query: 161 GWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKSAVKRSLHRATTG 220
           GW+LG +++L   +     P+  +PLGTGN+L  +FGWG   P   +S+V+  L +    
Sbjct: 140 GWLLGVVSDLNLSNPP---PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINA 199

Query: 221 QVRSLDSWHVSLLMPAGEK------VEPPHSMKLTEESDLN--EAVHELDERGDLPTKLD 280
           +   +D+W + + M   ++      ++ PHS+     SD    E  H             
Sbjct: 200 KEMKIDNWKILMRMKHPKEGSCDITLKLPHSLPRIFPSDQENMEGYH------------- 259

Query: 281 SYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSSYSCTQGWFFTSCSKD 340
           +Y G F+NYFS+GMDAQV+Y FH  R   P   +  + N+  Y   SCTQGWFF S    
Sbjct: 260 TYRGGFWNYFSLGMDAQVSYAFHSQRKLHPERFKNQLVNQSTYLKLSCTQGWFFASLF-H 319

Query: 341 PNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKR 400
           P+ + +  + +I +   N  +W  + IP S+RSIV LNL +++ G NPWG P P+    R
Sbjct: 320 PSSQNIAKLAKIQICDRN-GQWNDLHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDR 379

Query: 401 GFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDG 460
                  DDGL+EI G +  WH   ++        +AQA  +R+E + G  K AY+++DG
Sbjct: 380 SLTAPFVDDGLIEIVGFRNAWHGLILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDG 423

Query: 461 EPWKQPI-SKDLSTFVEI 470
           EPWKQP+ S D +  VEI
Sbjct: 440 EPWKQPLPSNDETVMVEI 423

BLAST of Lag0033801 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-72
Identity = 148/417 (35.49%), Postives = 224/417 (53.72%), Query Frame = 0

Query: 77  PPPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLDVKPHEFVQYGLRCLEILAD 136
           P P +P++VFIN +SGG+ G +L    + L++  QVFDL    P + ++     LE L D
Sbjct: 35  PTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD 94

Query: 137 RGDACAKQTRQKIRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGTGNDLSRSF 196
             D  A+Q R+K++++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +F
Sbjct: 95  --DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAF 154

Query: 197 GWGGSFPFAWKSAVKRSLHRATTGQVRSLDSWHVSLLMPAGEK---------VEPPHSMK 256
           GWG   P   ++AV+  L +    +V  +D+WH+ + M   ++         +E PHS+ 
Sbjct: 155 GWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSL- 214

Query: 257 LTEESDLNEAVHELDERGDLPTK-LDSYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQ 316
                    A H +    +L  +   ++ G F+NYFS+GMDAQ++Y FH  R   P   +
Sbjct: 215 --------HAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFK 274

Query: 317 GPVTNKLIYSSYSCTQGWFFTSCSKDPNLRGLKNILRIHVKKINCSEWEQIPIPSSVRSI 376
             + N+  Y    CTQGWF  S    P  R +  + ++ +   N  +W+ + IP S+RSI
Sbjct: 275 NQLVNQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSI 334

Query: 377 VALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAK 436
           V LNL +++ G NPWG P P     RG      DDGL+E+ G +  WH   ++       
Sbjct: 335 VCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGT 394

Query: 437 HIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPISKDLST-FVEIKRVSCQSLMINGD 483
            +AQA  IR E   G     +++MDGEPWKQP+  D  T  VEI  +   +++   D
Sbjct: 395 RLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435