Lag0033311 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033311
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
Locationchr11: 42677537 .. 42679981 (+)
RNA-Seq ExpressionLag0033311
SyntenyLag0033311
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTATTTAAGCAGCCTCTTAAGCGCTGCTGAGGACAGGGGTCTTCTAGCAGGTATATCTTTGGGAGACAACAATCCATCTCTCACTCACCTTCTTTTTGCAGATGATATCCTCCTTTTTATACAGGATAATGATGACTATCTGGATAACGCTGAAGGTTATTGGGAAATTGTCAAAATTAACCCAAATGAGTTTTGGGTTTTCAAAATTGACCTACTTTCTAAAAACTTGTTTTTTTAACCCATTTTATATCAAATTAGCTACTTTTTAGTGGGACCCACGATGTGAAAAGTCATTTTTATCCTTCTTCAATTTTCATCCTTCTCCAATTTTCATCCATGGTAGGAATTAAAATTTTCAACCAACCATTTGACCAACAAAGTATTTTTAAAAAATAAAATCAAACACATGAAATCAAACACACGAACCCACTTCAAAATTTTCATATTGTCAACGTTTTTTTGAAGTACAAGAGGATGATTTTACATGTTTTAGATAACAATAAATTTGTATAAGATCATATTTTTACAAAAATCTGGAAAAAAAAAGTGAATCCCGAGAAATCATGAATCCCGACCGAGAAATCATGAATCCCGACCGGGAAATCATGAATCCCGACCGGGATTTCTTGGTCGGGAAACCGACCAAGAAATTTTCCAGAAGTTTTTTTTTTTTTTTTTTTTTTTCGAAAGGTTTCTTCCCCACCACTAAATTATCAAATTCCCAAACTCCTCTTCCCCACTTAAAATCTTCTTCATAAATTTGTTTAAGAAAATCTTCTTTGTAAATTAACCATAAATTTGTTTAAGAAAATCTTCTTTGTAAATTAACCATAAATTTGTTTAAGAAAATCTTTCATAAATTTGTTTAAGAAAATCTTTCATAAAGTTGTTTAACAAAAAAAAAAAAAAAAGAAAAAGAAAAATAATTTATTAAAAAAAAAAAAAAAACCTAAATGGCAGCAAGCTGCCTTTTGTCCCGGTCAACTTCCCGGCCAGGAGCGTGACCGGGACAAAAAACAGTAATTAAGATTTCTTTTTATTTTCTTTTTTTTTTCTTCTCCTTCTACTTTTTTTTTCTTTCGTTTTCTGGGTTTACTGGTTGGACTTTTTTTTTTTTTTTTTTTTAGAAATCAAACTTTTATATATATTTTAGAGTTGTTTTTTATTTTTTTGATGGATGGAGAAGCTTCAATCTATTAGAAATACCCATTTTTTTCAAAATCTTTTGGGAAGAAAAACCCATTTTTTTTCTAAATTGTTTTGAAATAAAAAAAATTTCCAACCAAAATGTATCTTGTTTGAATTCTCTAACAAGGAAGAACGAAGATGTTTGTTGGTTTTTTTCCGGTTCGATTGGCTACGAAAACCCCTCTCCCTCTATGAAAAACTAAATAGAAAAACCCACAAAAATGAAGAGGTGTGGAGTCGGAGCTGCTGGACACGCCGGAGTCGTTGGAGTCGTCGGAGTCGAACGAGAGAGAAAGAACTGTCGGAGCCATTGGAGTCGTCGGAGCTGAGGGAGGAGAAGACGAAGTGAAGACGAAGGGGGTTTGTGTTTTTTGTTTCAATTTTGACCTTTTACCTTCAGGGTTATTGCTGTAAATTCACATTATTGGGTTTTATCAACTAGGTTAAAAAAACATGTTTTTAAGAATGAGGTTATTTTTGAAATCCAAAATCTTAAAAGGGTTAAAACTGACAATTTCCCAAGGTTATTCGAGCTTTTGAGATTGCTTCTGGCCTAAATATTAATCTCTCCAAATCCACCATATCAGGAATCAATGTCTCAAATATCAGACTCCAAGAAGTTGTGGCCAGATGGAACTGCGGCTCAAAGTCTCTTCCAATTACTTACCTAGGAGCCCCCCTCGGTGGAAAGCCCTCTTCTGAAATTTTCTGGTATTCAGTCTCTAAGAAGATTCAGCAGAAGTTAGATAAGTGGAGGTTTTCTTATATTTCAAAGGGAGGGAGAATAACTCTCATCCAATCAGTTCTAAGCAACCTTCCAACATACATGCTGTCGGTTTTCAAAGCCCCTGCTTCGGTGTGCAAAAAGATTGAAAAGGTCATTCGAAACCTCCTATGGGATGGTAATGACACTGCTTCAAACAACCACCTTGTTCGTTGGGACATTGTTGCTGCTCCCATATCCCATGGAGGCATCGGTATTTGGAGAATAAAGGACACCAACGAAGCCCTTCTTACGAAATGGCTTTGGCGCTTTGTTCACGGGAAAGACTCTCTTTGGAGGAGTCTTATTGAATTTAAATATAAAAAAGATCCTTTGGATATGTTCCCTACTGCCTCTAACCATGCTAGTTCTCGCTCCCGTGGAAGGCTATCATCTATTTTAAGGAGCTTTTTATTGCAAATTGTTGGTTTGTTGAGTCCAACAAGTTTACAGTGTACACATTTACTTGATGAAAAAGAAACAGAGTAA

mRNA sequence

ATGGATTATTTAAGCAGCCTCTTAAGCGCTGCTGAGGACAGGGGTCTTCTAGCAGGTATATCTTTGGGAGACAACAATCCATCTCTCACTCACCTTCTTTTTGCAGATGATATCCTCCTTTTTATACAGGATAATGATGACTATCTGGATAACGCTGAAGGTGTGGAGTCGGAGCTGCTGGACACGCCGGAGTCGTTGGAGTCGTCGGAGTCGAACGAGAGAGAAAGAACTGTCGGAGCCATTGGAGTCGTCGGAGCTGAGGGAGGAGAAGACGAAGTGAAGACGAAGGGGGTTATTCGAGCTTTTGAGATTGCTTCTGGCCTAAATATTAATCTCTCCAAATCCACCATATCAGGAATCAATGTCTCAAATATCAGACTCCAAGAAGTTGTGGCCAGATGGAACTGCGGCTCAAAGTCTCTTCCAATTACTTACCTAGGAGCCCCCCTCGGTGGAAAGCCCTCTTCTGAAATTTTCTGGTATTCAGTCTCTAAGAAGATTCAGCAGAAGTTAGATAAGTGGAGGTTTTCTTATATTTCAAAGGGAGGGAGAATAACTCTCATCCAATCAGTTCTAAGCAACCTTCCAACATACATGCTGTCGGTTTTCAAAGCCCCTGCTTCGGTGTGCAAAAAGATTGAAAAGGTCATTCGAAACCTCCTATGGGATGGTAATGACACTGCTTCAAACAACCACCTTGTTCGTTGGGACATTGTTGCTGCTCCCATATCCCATGGAGGCATCGGTATTTGGAGAATAAAGGACACCAACGAAGCCCTTCTTACGAAATGGCTTTGGCGCTTTGTTCACGGGAAAGACTCTCTTTGGAGGAGTCTTATTGAATTTAAATATAAAAAAGATCCTTTGGATATGTTCCCTACTGCCTCTAACCATGCTAGTTCTCGCTCCCGTGGAAGGCTATCATCTATTTTAAGGAGCTTTTTATTGCAAATTGTTGGTTTGTTGAGTCCAACAAGTTTACAGTGTACACATTTACTTGATGAAAAAGAAACAGAGTAA

Coding sequence (CDS)

ATGGATTATTTAAGCAGCCTCTTAAGCGCTGCTGAGGACAGGGGTCTTCTAGCAGGTATATCTTTGGGAGACAACAATCCATCTCTCACTCACCTTCTTTTTGCAGATGATATCCTCCTTTTTATACAGGATAATGATGACTATCTGGATAACGCTGAAGGTGTGGAGTCGGAGCTGCTGGACACGCCGGAGTCGTTGGAGTCGTCGGAGTCGAACGAGAGAGAAAGAACTGTCGGAGCCATTGGAGTCGTCGGAGCTGAGGGAGGAGAAGACGAAGTGAAGACGAAGGGGGTTATTCGAGCTTTTGAGATTGCTTCTGGCCTAAATATTAATCTCTCCAAATCCACCATATCAGGAATCAATGTCTCAAATATCAGACTCCAAGAAGTTGTGGCCAGATGGAACTGCGGCTCAAAGTCTCTTCCAATTACTTACCTAGGAGCCCCCCTCGGTGGAAAGCCCTCTTCTGAAATTTTCTGGTATTCAGTCTCTAAGAAGATTCAGCAGAAGTTAGATAAGTGGAGGTTTTCTTATATTTCAAAGGGAGGGAGAATAACTCTCATCCAATCAGTTCTAAGCAACCTTCCAACATACATGCTGTCGGTTTTCAAAGCCCCTGCTTCGGTGTGCAAAAAGATTGAAAAGGTCATTCGAAACCTCCTATGGGATGGTAATGACACTGCTTCAAACAACCACCTTGTTCGTTGGGACATTGTTGCTGCTCCCATATCCCATGGAGGCATCGGTATTTGGAGAATAAAGGACACCAACGAAGCCCTTCTTACGAAATGGCTTTGGCGCTTTGTTCACGGGAAAGACTCTCTTTGGAGGAGTCTTATTGAATTTAAATATAAAAAAGATCCTTTGGATATGTTCCCTACTGCCTCTAACCATGCTAGTTCTCGCTCCCGTGGAAGGCTATCATCTATTTTAAGGAGCTTTTTATTGCAAATTGTTGGTTTGTTGAGTCCAACAAGTTTACAGTGTACACATTTACTTGATGAAAAAGAAACAGAGTAA

Protein sequence

MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELLDTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGINVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYISKGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVAAPISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHASSRSRGRLSSILRSFLLQIVGLLSPTSLQCTHLLDEKETE
Homology
BLAST of Lag0033311 vs. NCBI nr
Match: KAA0041367.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 254.6 bits (649), Expect = 1.2e-63
Identity = 130/303 (42.90%), Postives = 180/303 (59.41%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ + + N +LTHLLFADDILLF++D++  + N         
Sbjct: 319 MDYISRLLNSVGEK--IKGVKM-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 378

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 379 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 438

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK +++ FW +V +KI +KL  W++S +S
Sbjct: 439 NVDAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKTFWKNVEEKINKKLASWKYSMLS 498

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 499 KGGKITLIKSSLASLPTYQLSIFKAPVSACKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 558

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +P   GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 559 SPKERGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 575

Query: 301 SRS 304
           SRS
Sbjct: 619 SRS 575

BLAST of Lag0033311 vs. NCBI nr
Match: TYK06777.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 251.5 bits (641), Expect = 1.0e-62
Identity = 130/303 (42.90%), Postives = 179/303 (59.08%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 558 MDYISRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 617

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 618 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 677

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK +++ FW +V +KI +KL  W++S +S
Sbjct: 678 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQTTKAFWKNVEEKINKKLASWKYSMLS 737

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 738 KGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 797

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 798 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 814

Query: 301 SRS 304
           SRS
Sbjct: 858 SRS 814

BLAST of Lag0033311 vs. NCBI nr
Match: KAA0046762.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 251.5 bits (641), Expect = 1.0e-62
Identity = 138/313 (44.09%), Postives = 187/313 (59.74%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDYLS LLS  E  G + G+S   +N +++H+LFADDILLFI+DND +L+N         
Sbjct: 495 MDYLSRLLSHLEITGAIKGVSF-SSNCNISHILFADDILLFIEDNDFFLNN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              +  +  FE ASGL INL KS +  +
Sbjct: 555 ----------------------------------LRMALSLFERASGLKINLLKSALVPM 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NVS IR +E  + W   S+SLP++YLG PLGG P S +FW +V +KIQ+KL+ W+++ IS
Sbjct: 615 NVSEIRAKECASFWGISSQSLPLSYLGVPLGGNPKSRLFWSNVEEKIQKKLNNWKYAQIS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGGR+TLI+S LS+LPTY LSVF+AP+  CK IEK  RN LW GN+++   HL+ W  V 
Sbjct: 675 KGGRLTLIKSTLSSLPTYQLSVFQAPSLTCKNIEKFWRNFLWKGNNSSEGFHLINWTKVT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFP--TASNH 300
              + GG+GI R+  TN+ALLTKWLWR++    +LWR LI+ KYK     ++P    SN 
Sbjct: 735 KSKAEGGLGISRVHVTNKALLTKWLWRYLSEPKALWRRLIQCKYK----GIYPGDIPSNI 759

Query: 301 ASSRSRGRLSSIL 312
           +SS S+    SI+
Sbjct: 795 SSSTSKAPWRSII 759

BLAST of Lag0033311 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 250.4 bits (638), Expect = 2.3e-62
Identity = 130/303 (42.90%), Postives = 178/303 (58.75%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 495 MDYMSRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 555 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK  ++ FW +V +KI +KL  W++S +S
Sbjct: 615 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 675 KGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 735 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 751

Query: 301 SRS 304
           SRS
Sbjct: 795 SRS 751

BLAST of Lag0033311 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 248.8 bits (634), Expect = 6.6e-62
Identity = 129/303 (42.57%), Postives = 177/303 (58.42%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 495 MDYMSRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 555 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK  ++ FW +V +KI +KL  W++S +S
Sbjct: 615 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FK P S CK IEK  RN LW         HLV W  + 
Sbjct: 675 KGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 735 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 751

Query: 301 SRS 304
           SRS
Sbjct: 795 SRS 751

BLAST of Lag0033311 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.3e-19
Identity = 39/122 (31.97%), Postives = 70/122 (57.38%), Query Frame = 0

Query: 163 VSKKIQQKLDKWRFSYISKGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLW 222
           + +++  ++  WR   +S  GR+TL ++VLS++P + +S    P S+  +++++ R  LW
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 223 DGNDTASNNHLVRWDIVAAPISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEF 282
                    HLV+W  V +P   GG+G+   K  N AL++K  WR +  K+SLW  +++ 
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 283 KY 285
           KY
Sbjct: 136 KY 137

BLAST of Lag0033311 vs. ExPASy TrEMBL
Match: A0A5A7TI93 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00120 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 5.8e-64
Identity = 130/303 (42.90%), Postives = 180/303 (59.41%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ + + N +LTHLLFADDILLF++D++  + N         
Sbjct: 319 MDYISRLLNSVGEK--IKGVKM-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 378

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 379 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 438

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK +++ FW +V +KI +KL  W++S +S
Sbjct: 439 NVDAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKTFWKNVEEKINKKLASWKYSMLS 498

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 499 KGGKITLIKSSLASLPTYQLSIFKAPVSACKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 558

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +P   GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 559 SPKERGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 575

Query: 301 SRS 304
           SRS
Sbjct: 619 SRS 575

BLAST of Lag0033311 vs. ExPASy TrEMBL
Match: A0A5D3C4J1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00790 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 130/303 (42.90%), Postives = 179/303 (59.08%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 558 MDYISRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 617

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 618 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 677

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK +++ FW +V +KI +KL  W++S +S
Sbjct: 678 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQTTKAFWKNVEEKINKKLASWKYSMLS 737

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 738 KGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 797

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 798 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 814

Query: 301 SRS 304
           SRS
Sbjct: 858 SRS 814

BLAST of Lag0033311 vs. ExPASy TrEMBL
Match: A0A5A7TTK1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00450 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 138/313 (44.09%), Postives = 187/313 (59.74%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDYLS LLS  E  G + G+S   +N +++H+LFADDILLFI+DND +L+N         
Sbjct: 495 MDYLSRLLSHLEITGAIKGVSF-SSNCNISHILFADDILLFIEDNDFFLNN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              +  +  FE ASGL INL KS +  +
Sbjct: 555 ----------------------------------LRMALSLFERASGLKINLLKSALVPM 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NVS IR +E  + W   S+SLP++YLG PLGG P S +FW +V +KIQ+KL+ W+++ IS
Sbjct: 615 NVSEIRAKECASFWGISSQSLPLSYLGVPLGGNPKSRLFWSNVEEKIQKKLNNWKYAQIS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGGR+TLI+S LS+LPTY LSVF+AP+  CK IEK  RN LW GN+++   HL+ W  V 
Sbjct: 675 KGGRLTLIKSTLSSLPTYQLSVFQAPSLTCKNIEKFWRNFLWKGNNSSEGFHLINWTKVT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFP--TASNH 300
              + GG+GI R+  TN+ALLTKWLWR++    +LWR LI+ KYK     ++P    SN 
Sbjct: 735 KSKAEGGLGISRVHVTNKALLTKWLWRYLSEPKALWRRLIQCKYK----GIYPGDIPSNI 759

Query: 301 ASSRSRGRLSSIL 312
           +SS S+    SI+
Sbjct: 795 SSSTSKAPWRSII 759

BLAST of Lag0033311 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.1e-62
Identity = 130/303 (42.90%), Postives = 178/303 (58.75%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 495 MDYMSRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 555 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK  ++ FW +V +KI +KL  W++S +S
Sbjct: 615 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FKAP S CK IEK  RN LW         HLV W  + 
Sbjct: 675 KGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 735 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 751

Query: 301 SRS 304
           SRS
Sbjct: 795 SRS 751

BLAST of Lag0033311 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 3.2e-62
Identity = 129/303 (42.57%), Postives = 177/303 (58.42%), Query Frame = 0

Query: 1   MDYLSSLLSAAEDRGLLAGISLGDNNPSLTHLLFADDILLFIQDNDDYLDNAEGVESELL 60
           MDY+S LL++  ++  + G+ L + N +LTHLLFADDILLF++D++  + N         
Sbjct: 495 MDYMSRLLNSVGEK--IKGVKL-EGNINLTHLLFADDILLFVEDDEHSIQN--------- 554

Query: 61  DTPESLESSESNERERTVGAIGVVGAEGGEDEVKTKGVIRAFEIASGLNINLSKSTISGI 120
                                              K +I  F++ASGL+INL+KSTIS I
Sbjct: 555 ----------------------------------LKNIINLFQLASGLSINLNKSTISPI 614

Query: 121 NVSNIRLQEVVARWNCGSKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYIS 180
           NV   R +++ ++W   +K LPI YLG PLGGK  ++ FW +V +KI +KL  W++S +S
Sbjct: 615 NVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKINKKLASWKYSMLS 674

Query: 181 KGGRITLIQSVLSNLPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVA 240
           KGG+ITLI+S L++LPTY LS+FK P S CK IEK  RN LW         HLV W  + 
Sbjct: 675 KGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPETHKLHLVNWAKIT 734

Query: 241 APISHGGIGIWRIKDTNEALLTKWLWRFVHGKDSLWRSLIEFKYKKDPLDMFPTASNHAS 300
           +    GG+GI R+KDTN ALLTKWLWR++H    LW+ +I  KY+       P   NH+S
Sbjct: 735 SSKEKGGLGISRLKDTNFALLTKWLWRYIHEDSPLWKKIINAKYRSLSKGDIPCVCNHSS 751

Query: 301 SRS 304
           SRS
Sbjct: 795 SRS 751

BLAST of Lag0033311 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.8e-17
Identity = 42/139 (30.22%), Postives = 73/139 (52.52%), Query Frame = 0

Query: 138 SKSLPITYLGAPLGGKPSSEIFWYSVSKKIQQKLDKWRFSYISKGGRITLIQSVLSNLPT 197
           S +LP+ YLG PL  K  +   +  + +KI+ ++ KW   ++S  GR+ LI SV+ +L  
Sbjct: 20  SGALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTN 79

Query: 198 YMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVAAPISHGGIGIWRIKDTN 257
           + +S F+ P++  K+I+ +  + LW G +  +    V W  V  P   GG+GI  +K+ N
Sbjct: 80  FWMSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEAN 139

Query: 258 E---------ALLTKWLWR 268
           +           L  W+W+
Sbjct: 140 KGSFWSISGNTTLGSWMWK 158

BLAST of Lag0033311 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-11
Identity = 35/98 (35.71%), Postives = 52/98 (53.06%), Query Frame = 0

Query: 195 LPTYMLSVFKAPASVCKKIEKVIRNLLWDGNDTASNNHLVRWDIVAAPISHGGIGIWRIK 254
           LPTY ++ F  P +VCK+I  V+ +  W     A   H   WD ++   + GGIG   I+
Sbjct: 3   LPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIE 62

Query: 255 DTNEALLTKWLWRFVHGKDSLWRSLIEFKY--KKDPLD 291
             N ALL K +WR +   +SL   + + +Y  K DPL+
Sbjct: 63  AFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLN 100

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0041367.11.2e-6342.90LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
TYK06777.11.0e-6242.90LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0046762.11.0e-6244.09LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0039770.12.3e-6242.90LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK247... [more]
XP_016902461.16.6e-6242.57PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
Match NameE-valueIdentityDescription
P0C2F69.3e-1931.97Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5A7TI935.8e-6442.90LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C4J14.9e-6342.90LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TTK14.9e-6344.09LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DM721.1e-6242.90LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K53.2e-6242.57LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
Match NameE-valueIdentityDescription
AT3G24255.11.8e-1730.22RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G29090.11.1e-1135.71Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 335..339
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 2..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033311.1Lag0033311.1mRNA