Lag0033011 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033011
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	vacuolar protein sorting-associated protein 55 homolog
Location	chr11: 39929302 .. 39930335 (-)
RNA-Seq Expression	Lag0033011
Synteny	Lag0033011

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGACTTGGGAAACTGCTTGCATATGAGCAAAGTTGTAACGCTAGGAATCTTGGTTTCTGGAGGAATTGTGTTACAGTTTTTGGTCAGTAGATTCTTTCCTTGACTAACGCTTGCTCAATATCTTCACCATTTTTATTTTCTTAATCTTTCACAGAGAATTTGCTGTGTGCAGGCCCCTTTGCTATTTCTTATACAGAATTCTGAATTGTTCATTTGCGTGTGGTAATGGCAGGCATGTGCTTTGTACAATAACTGGTGGCCATTGCTGAGCGGTAAGATTGAAAAATGGTAGTTCTGAGCATTATTTTCAATGTATGATATCTTTGAAACTTTCTCAGCCGTTGAACCCGAATTTGTTTCAGTAGCCATAGCATCTTACTGGGAAATTTTTGTAGAAAAACAGTCATTCTGGTATATCTTAAGATATAATATCAGTTTTTTACCTTTTGGCCTTTGATGGGAAGTAGACTTGACATGGAATTAGAATTGAGGGACCTATCAAACATAAAATTTAAAGTTCAATAGTCTAAGAAGTATGTGGACTAAGTAGACACAACACAACACTACAAGTTCACAAACTTATTTAGGTACTAAATATATCTTTGAAACTTTCCCGGTAGTTGATTACGATTTTATTTCACCATTCTTGCAGTGGTCATGTATGTTCTTCTCCCAATGCCTTTGCTGTTTTTCGCTGGCTCTGATAGTTCTTCGCTATTTTCCGAATCTGGAGACAGGTTTGTTTCTGATTATCATATTTCGTTCCTAGACAATTAAAATCCATTTCTAGACTAGCCAGTCGATAACCAATTTCTTTTTAACGATTAACGAAACAGCTGGGTGAATGCAACTAAATTTTTAACTGGAGCTTCAGCAGTTGGAAGCATTGCCATACCTGTAATTTTGAAGCATGCTGATATTATCGGCTGGGGTGCACTTGCACTGGACCTCTCGTCCCTTATAGTTTTTGTGGTGGCCATTTTCTGTTATCTCTGGATGAGTAATGAAGACGACTATAATATACTCTAA

mRNA sequence

ATGGCAGACTTGGGAAACTGCTTGCATATGAGCAAAGTTGTAACGCTAGGAATCTTGGTTTCTGGAGGAATTGTGTTACAGTTTTTGGCATGTGCTTTGTACAATAACTGGTGGCCATTGCTGAGCGTGGTCATGTATGTTCTTCTCCCAATGCCTTTGCTGTTTTTCGCTGGCTCTGATAGTTCTTCGCTATTTTCCGAATCTGGAGACAGCTGGGTGAATGCAACTAAATTTTTAACTGGAGCTTCAGCAGTTGGAAGCATTGCCATACCTGTAATTTTGAAGCATGCTGATATTATCGGCTGGGGTGCACTTGCACTGGACCTCTCGTCCCTTATAGTTTTTGTGGTGGCCATTTTCTGTTATCTCTGGATGAGTAATGAAGACGACTATAATATACTCTAA

Coding sequence (CDS)

ATGGCAGACTTGGGAAACTGCTTGCATATGAGCAAAGTTGTAACGCTAGGAATCTTGGTTTCTGGAGGAATTGTGTTACAGTTTTTGGCATGTGCTTTGTACAATAACTGGTGGCCATTGCTGAGCGTGGTCATGTATGTTCTTCTCCCAATGCCTTTGCTGTTTTTCGCTGGCTCTGATAGTTCTTCGCTATTTTCCGAATCTGGAGACAGCTGGGTGAATGCAACTAAATTTTTAACTGGAGCTTCAGCAGTTGGAAGCATTGCCATACCTGTAATTTTGAAGCATGCTGATATTATCGGCTGGGGTGCACTTGCACTGGACCTCTCGTCCCTTATAGTTTTTGTGGTGGCCATTTTCTGTTATCTCTGGATGAGTAATGAAGACGACTATAATATACTCTAA

Protein sequence

MADLGNCLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSDSSSLFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFCYLWMSNEDDYNIL

Homology

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BLAST of Lag0033011 vs. NCBI nr
Match: XP_022157395.1 (vacuolar protein sorting-associated protein 55 homolog [Momordica charantia] >XP_022157399.1 vacuolar protein sorting-associated protein 55 homolog [Momordica charantia])

HSP 1 Score: 239.2 bits (609), Expect = 2.1e-59
Identity = 119/134 (88.81%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 1   MADLGNCLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSD 60
           MADLG C HMSK+VTLG+LVSGGIVLQFLAC LY NWWPLLSV+MY+LLPMPLLFFAGSD
Sbjct: 1   MADLGYCWHMSKLVTLGVLVSGGIVLQFLACVLYXNWWPLLSVIMYILLPMPLLFFAGSD 60

Query: 61  SSSLFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIF 120
           +SSL SESGDSWVNATKFLTGAS VGSIAIPVILKHA+IIGWGALALDLSSL+VFVVAI 
Sbjct: 61  TSSLSSESGDSWVNATKFLTGASTVGSIAIPVILKHANIIGWGALALDLSSLLVFVVAIL 120

Query: 121 CYLWMSNEDDYNIL 135
           CYLWMSNEDDYN L
Sbjct: 121 CYLWMSNEDDYNTL 134

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BLAST of Lag0033011 vs. NCBI nr
Match: XP_004150170.1 (vacuolar protein sorting-associated protein 55 homolog [Cucumis sativus] >XP_031739448.1 vacuolar protein sorting-associated protein 55 homolog [Cucumis sativus] >XP_031739449.1 vacuolar protein sorting-associated protein 55 homolog [Cucumis sativus] >KGN55797.1 hypothetical protein Csa_009853 [Cucumis sativus])

HSP 1 Score: 206.5 bits (524), Expect = 1.5e-49
Identity = 101/132 (76.52%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 2   ADLGNCLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSDS 61
           A L  CL M KV  L ILVSGGIV+Q LACALYNNWWP+LSV+MYVLLPMPLLFFAGSDS
Sbjct: 6   ASLQACLGMGKVAFLAILVSGGIVMQILACALYNNWWPMLSVIMYVLLPMPLLFFAGSDS 65

Query: 62  SSLFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFC 121
           SSL+++S DSW+NATKFLTGAS VGSIAIPVILKHA IIGWGA+A+DLSS +VFVVAI C
Sbjct: 66  SSLYTDSNDSWINATKFLTGASTVGSIAIPVILKHAGIIGWGAMAMDLSSFVVFVVAILC 125

Query: 122 YLWMSNEDDYNI 134
           ++ MS +DDY++
Sbjct: 126 FMGMSEDDDYSM 137

[Close]

BLAST of Lag0033011 vs. NCBI nr
Match: XP_022155500.1 (vacuolar protein sorting-associated protein 55 homolog [Momordica charantia] >XP_022155501.1 vacuolar protein sorting-associated protein 55 homolog [Momordica charantia])

HSP 1 Score: 204.5 bits (519), Expect = 5.6e-49
Identity = 100/134 (74.63%), Postives = 114/134 (85.07%), Query Frame = 0


Query: 1   MADLGN----CLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFF 60
           MADL      C  M KV  L ILVSGGIV+Q LACALYNNWWP+LSV+MYVLLPMPLLFF
Sbjct: 1   MADLPGSLQACFSMGKVAFLAILVSGGIVMQILACALYNNWWPMLSVIMYVLLPMPLLFF 60

Query: 61  AGSDSSSLFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFV 120
            GSDSSSL++ES DSW+NATKFLTGAS +GSIAIP+ILKHA IIGWGALA+DLSS +VFV
Sbjct: 61  GGSDSSSLYTESNDSWINATKFLTGASTIGSIAIPIILKHAGIIGWGALAMDLSSFLVFV 120

Query: 121 VAIFCYLWMSNEDD 131
           +AI C+LWM+ +DD
Sbjct: 121 IAILCFLWMNEDDD 134

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BLAST of Lag0033011 vs. NCBI nr
Match: XP_008448742.1 (PREDICTED: vacuolar protein sorting-associated protein 55 homolog [Cucumis melo] >XP_008448744.1 PREDICTED: vacuolar protein sorting-associated protein 55 homolog [Cucumis melo] >XP_016900672.1 PREDICTED: vacuolar protein sorting-associated protein 55 homolog [Cucumis melo] >XP_016900673.1 PREDICTED: vacuolar protein sorting-associated protein 55 homolog [Cucumis melo] >KAA0053070.1 vacuolar protein sorting-associated protein 55-like protein [Cucumis melo var. makuwa] >TYK11525.1 vacuolar protein sorting-associated protein 55-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 204.1 bits (518), Expect = 7.3e-49
Identity = 99/127 (77.95%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 7   CLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSDSSSLFS 66
           CL M KV  L ILVSGGIV+Q LACALYNNWWP+LSV+MYVLLPMPLLFFAGSDSSSL++
Sbjct: 11  CLGMGKVAFLAILVSGGIVMQILACALYNNWWPMLSVIMYVLLPMPLLFFAGSDSSSLYT 70

Query: 67  ESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFCYLWMS 126
           +S DSW+NATKFLTGAS VGSIAIPVILKHA IIGWGA+A+DLSS +VFVVAI C++ MS
Sbjct: 71  DSNDSWINATKFLTGASTVGSIAIPVILKHAGIIGWGAMAMDLSSFVVFVVAILCFMGMS 130

Query: 127 NEDDYNI 134
            +DDY++
Sbjct: 131 EDDDYSM 137

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BLAST of Lag0033011 vs. NCBI nr
Match: XP_021599879.1 (vacuolar protein sorting-associated protein 55 homolog [Manihot esculenta] >OAY24482.1 hypothetical protein MANES_17G019200v8 [Manihot esculenta] >OAY24520.2 hypothetical protein MANES_17G019200v8 [Manihot esculenta])

HSP 1 Score: 203.4 bits (516), Expect = 1.3e-48
Identity = 104/138 (75.36%), Postives = 117/138 (84.78%), Query Frame = 0


Query: 1   MADLGN----CLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFF 60
           MADL      CL   K+  L ILVSGGI+LQ LACALYNNWWP+LSV+MYVLLPMPLLFF
Sbjct: 1   MADLPGYVRACLQSGKLAFLAILVSGGIILQILACALYNNWWPMLSVIMYVLLPMPLLFF 60

Query: 61  AGSDSSSLFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFV 120
           AGSDSSSLFSES +SWVNATKFLTGASAVGSIAIP ILKHA +IGWGALA++LSS  VFV
Sbjct: 61  AGSDSSSLFSESNNSWVNATKFLTGASAVGSIAIPAILKHAGVIGWGALAMELSSFFVFV 120

Query: 121 VAIFCYLWMSNEDDYNIL 135
           +AI CY+ M++ED Y+IL
Sbjct: 121 IAIMCYISMNDEDGYSIL 138

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BLAST of Lag0033011 vs. ExPASy Swiss-Prot
Match: Q9AST6 (Vacuolar protein sorting-associated protein 55 homolog OS=Arabidopsis thaliana OX=3702 GN=At1g32410 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 3.9e-45
Identity = 91/127 (71.65%), Postives = 106/127 (83.46%), Query Frame = 0


Query: 4   LGNCLHMSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSDSSS 63
           L  CL M K+  L ILVS GIVLQ LACAL+NNWWP+LSV+MYVLLPMPLLFF GSDS+S
Sbjct: 8   LRTCLDMGKIAFLAILVSTGIVLQILACALFNNWWPMLSVIMYVLLPMPLLFFGGSDSTS 67

Query: 64  LFSESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFCYL 123
           LF+ES +SW+NA KFLTGASAVGS+AIP ILKHA +IGWGALALDLSS +VF+VAI  Y+
Sbjct: 68  LFNESDNSWINAAKFLTGASAVGSVAIPSILKHAGLIGWGALALDLSSYVVFLVAILGYI 127

Query: 124 WMSNEDD 131
            + +  D
Sbjct: 128 CIGDASD 134

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BLAST of Lag0033011 vs. ExPASy Swiss-Prot
Match: P47111 (Vacuolar protein sorting-associated protein 55 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS55 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-09
Identity = 37/128 (28.91%), Postives = 73/128 (57.03%), Query Frame = 0


Query: 10  MSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMP-LLFFAGS--DSSSLFS 69
           ++K+++L   ++ G +L  L+CAL++N++PL  +++++L P+P  +F AG+   +S   S
Sbjct: 9   LTKIISLSGFLALGFLLVILSCALFHNYYPLFDILIFLLAPIPNTIFNAGNKYHTSDFMS 68

Query: 70  ESGDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFCYLWM- 129
           +S ++  +   FLTG      IA+PV+  H  +IG  +  + +   ++   +I  + W  
Sbjct: 69  DSSNTGQDLAHFLTGMLVTSGIALPVVFYHCQLIGHLSCIMCMIGGLIIYSSIVIFKWFF 128

Query: 130 ---SNEDD 131
               NEDD
Sbjct: 129 KKDFNEDD 136

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BLAST of Lag0033011 vs. ExPASy Swiss-Prot
Match: Q9UUH1 (Vacuolar protein sorting-associated protein 55 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps55 PE=3 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 34/125 (27.20%), Postives = 69/125 (55.20%), Query Frame = 0


Query: 10  MSKVVTLGILVSGGIVLQFLACALYNNWWPLLSVVMYVLLPMP-LLFFAGSDSSSLFSES 69
           + K++ L  +++ G +L  L+CAL+ NW+PLL V+ ++L P+P LL    S S     E 
Sbjct: 4   LRKIIGLSSVLAVGFMLVILSCALFKNWYPLLIVIPFILAPLPNLLTKKYSTSHDFLQEE 63

Query: 70  GDSWVNATKFLTGASAVGSIAIPVILKHADIIGWGALALDL--SSLIVFVVAIFCYLWMS 129
             + ++  +F  GA+     A+P++  +  +IG  A  +     S+I  V+ ++   ++ 
Sbjct: 64  DRNLLDFGRFTFGATICTGFALPIVFVNVGLIGTAAATMSCVGGSIIFLVITLYSQAFVQ 123

Query: 130 NEDDY 132
           +E+++
Sbjct: 124 HEEEF 128

[Close]

BLAST of Lag0033011 vs. ExPASy Swiss-Prot
Match: Q54VP1 (Vacuolar protein sorting-associated protein 55 homolog OS=Dictyostelium discoideum OX=44689 GN=vps55 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-07
Identity = 36/113 (31.86%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 23  GIVLQFLACALYNNWWPLLSVVMYVLLPMPLLFFAGSDSSSLFSESGDSWVNATKFLTGA 82
           G++   LAC + ++ +P++ V  Y L P P +     DS   FS    ++ +   FLTG 
Sbjct: 15  GLLFNILACIVSHSGYPIIVVASYFLAPFPNILCRNRDS---FSSEKGTFEDIGLFLTGL 74

Query: 83  SAVGSIAIPVILKHADIIGWGALALDLSSLIVFVVAIFCYLWM---SNEDDYN 133
                 AIP+IL H+DII   ALA  ++  +     I  +LW     N++D N
Sbjct: 75  FITSGFAIPMILAHSDIISGKALAFSMAGGVTVYATIITFLWFFNRHNDEDNN 124

[Close]

BLAST of Lag0033011 vs. ExPASy Swiss-Prot
Match: Q32PD8 (Leptin receptor overlapping transcript-like 1 OS=Bos taurus OX=9913 GN=LEPROTL1 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.1e-06
Identity = 35/117 (29.91%), Postives = 59/117 (50.43%), Query Frame = 0


Query: 23  GIVLQFLACAL--YNNWWPLLSVVMYVLLPMPLLFFAGSDSSSLFSESGDSWVNATK--- 82
           G++   L CAL  YN +WPL  +  Y+L P+P         +    +  D+  NA K   
Sbjct: 17  GLMFLMLGCALPIYNQYWPLFVLFFYILSPIPYCI------ARRLVDDTDAMSNACKELA 76

Query: 83  -FLTGASAVGSIAIPVILKHADIIGWGALALDLS-SLIVFVVAIFCYLWMSNEDDYN 133
            FLT    V +  +P++   A++I WGA AL L+ + ++F   +  +L   + DD++
Sbjct: 77  IFLTTGIVVSAFGLPIVFARANLIEWGACALVLTGNTVIFATILGFFLVFGSNDDFS 127

The following BLAST results are available for this feature:

BLAST of Lag0033011 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157395.1	2.1e-59	88.81	vacuolar protein sorting-associated protein 55 homolog [Momordica charantia] >XP...	[more]
XP_004150170.1	1.5e-49	76.52	vacuolar protein sorting-associated protein 55 homolog [Cucumis sativus] >XP_031...	[more]
XP_022155500.1	5.6e-49	74.63	vacuolar protein sorting-associated protein 55 homolog [Momordica charantia] >XP...	[more]
XP_008448742.1	7.3e-49	77.95	PREDICTED: vacuolar protein sorting-associated protein 55 homolog [Cucumis melo]...	[more]
XP_021599879.1	1.3e-48	75.36	vacuolar protein sorting-associated protein 55 homolog [Manihot esculenta] >OAY2...	[more]

BLAST of Lag0033011 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AST6	3.9e-45	71.65	Vacuolar protein sorting-associated protein 55 homolog OS=Arabidopsis thaliana O...	[more]
P47111	4.5e-09	28.91	Vacuolar protein sorting-associated protein 55 OS=Saccharomyces cerevisiae (stra...	[more]
Q9UUH1	1.7e-08	27.20	Vacuolar protein sorting-associated protein 55 OS=Schizosaccharomyces pombe (str...	[more]
Q54VP1	1.5e-07	31.86	Vacuolar protein sorting-associated protein 55 homolog OS=Dictyostelium discoide...	[more]
Q32PD8	2.1e-06	29.91	Leptin receptor overlapping transcript-like 1 OS=Bos taurus OX=9913 GN=LEPROTL1 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007262	Vps55/LEPROT	PFAM	PF04133	Vps55	coord: 13..129 e-value: 1.2E-33 score: 115.5
IPR007262	Vps55/LEPROT	PANTHER	PTHR12050	LEPTIN RECEPTOR-RELATED	coord: 4..131
None	No IPR available	PANTHER	PTHR12050:SF1	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 55 HOMOLOG	coord: 4..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033011.1	Lag0033011.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway
cellular_component	GO:0005768	endosome
cellular_component	GO:0016021	integral component of membrane