Lag0033006 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0033006
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	chr11: 39873123 .. 39873674 (+)
RNA-Seq Expression	Lag0033006
Synteny	Lag0033006

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAACCACTTCTCAAAATCGCTTCTAGTCCTAGTGCTTTTCTCTCTTCAAACACTCTTCCAATTCGGTTCCTCTCTCGACATCGTCAAACGCTCTTGCAAATTAGCAGCAAGAACAGACCCATACGTCGATTACAAACTGTGTGTCCGAAGCCTAAAGGCCAATCCGAATTCGAAAGACGCAACGTTCAAAGAATTGGTGCTGATTTCGATCAAGCAATCGAAGGCCAACGCCACGGGAATCGGCGCGGAGATTTCAGAGCTTCTGAAGGGCAGCGGAAAATTGGGGAAATATTCGTTGAGTTGTTTGAATAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATTTGAAATTGGCTTTGGCTGCTGTGAAAATGGCGGATTATGAAACTGCAAATACAGAGGTCAGCGCCGCCATGGATGCACCTGTGACTTGTGAAGAAGGATATAAGGAGAAGGATGGGGAAGCTTCTCCTTTGACAGAGATGAATGATGGGTTCTTTCAGTTGGCTGCAATTTCTCTGGCTTTTATCCATATGTGTCACTGA

mRNA sequence

ATGAGAAACCACTTCTCAAAATCGCTTCTAGTCCTAGTGCTTTTCTCTCTTCAAACACTCTTCCAATTCGGTTCCTCTCTCGACATCGTCAAACGCTCTTGCAAATTAGCAGCAAGAACAGACCCATACGTCGATTACAAACTGTGTGTCCGAAGCCTAAAGGCCAATCCGAATTCGAAAGACGCAACGTTCAAAGAATTGGTGCTGATTTCGATCAAGCAATCGAAGGCCAACGCCACGGGAATCGGCGCGGAGATTTCAGAGCTTCTGAAGGGCAGCGGAAAATTGGGGAAATATTCGTTGAGTTGTTTGAATAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATTTGAAATTGGCTTTGGCTGCTGTGAAAATGGCGGATTATGAAACTGCAAATACAGAGGTCAGCGCCGCCATGGATGCACCTGTGACTTGTGAAGAAGGATATAAGGAGAAGGATGGGGAAGCTTCTCCTTTGACAGAGATGAATGATGGGTTCTTTCAGTTGGCTGCAATTTCTCTGGCTTTTATCCATATGTGTCACTGA

Coding sequence (CDS)

ATGAGAAACCACTTCTCAAAATCGCTTCTAGTCCTAGTGCTTTTCTCTCTTCAAACACTCTTCCAATTCGGTTCCTCTCTCGACATCGTCAAACGCTCTTGCAAATTAGCAGCAAGAACAGACCCATACGTCGATTACAAACTGTGTGTCCGAAGCCTAAAGGCCAATCCGAATTCGAAAGACGCAACGTTCAAAGAATTGGTGCTGATTTCGATCAAGCAATCGAAGGCCAACGCCACGGGAATCGGCGCGGAGATTTCAGAGCTTCTGAAGGGCAGCGGAAAATTGGGGAAATATTCGTTGAGTTGTTTGAATAGCTGTTTGGAGCTTTATTCTGAGGCTGTTTCTGATTTGAAATTGGCTTTGGCTGCTGTGAAAATGGCGGATTATGAAACTGCAAATACAGAGGTCAGCGCCGCCATGGATGCACCTGTGACTTGTGAAGAAGGATATAAGGAGAAGGATGGGGAAGCTTCTCCTTTGACAGAGATGAATGATGGGTTCTTTCAGTTGGCTGCAATTTCTCTGGCTTTTATCCATATGTGTCACTGA

Protein sequence

MRNHFSKSLLVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDATFKELVLISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKLALAAVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIHMCH

Homology

[Close]

BLAST of Lag0033006 vs. NCBI nr
Match: XP_022958457.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 277.7 bits (709), Expect = 7.1e-71
Identity = 144/182 (79.12%), Postives = 159/182 (87.36%), Query Frame = 0


Query: 1   MRNHFSKSLLVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSK 60
           M+ HFS S  +L+LFSL TLFQFG  LDIV+RSCKLAA+ DPYVDYKLCVRSL+ANP+SK
Sbjct: 1   MKTHFSTS--ILMLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSK 60

Query: 61  DATFKELVLISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKL 120
           DATFK+LVLISI+QSKANAT IG+EI+ LLKG GK GKYS SCL SCLE+YSEAVSDLKL
Sbjct: 61  DATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKL 120

Query: 121 ALAAVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIH 180
            L AVKM DYETANTEVSAAMDAP+TCE+GY EKDGE SPLTE N GFFQL AISLAFI 
Sbjct: 121 GLVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFIS 180

Query: 181 MC 183
           +C
Sbjct: 181 LC 180

[Close]

BLAST of Lag0033006 vs. NCBI nr
Match: XP_022995887.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 276.6 bits (706), Expect = 1.6e-70
Identity = 144/182 (79.12%), Postives = 158/182 (86.81%), Query Frame = 0


Query: 1   MRNHFSKSLLVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSK 60
           M+ HF  S  +LVLFSL TLFQFG SLDIV+RSCKLAA+ DPYVDYKLCV+SL+ANP+SK
Sbjct: 1   MKTHFPAS--ILVLFSLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSK 60

Query: 61  DATFKELVLISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKL 120
           DATFK+LVLISI+QSKANAT IG+EI+ LLKG GK GKYS SCL SCLE+YSEAVSDLKL
Sbjct: 61  DATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSSSCLESCLEVYSEAVSDLKL 120

Query: 121 ALAAVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIH 180
            L AVKM DYETANTEVSAAMDAP+TCE GY EKDGE SPLTE N GFFQL AISLAFI 
Sbjct: 121 GLVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFIS 180

Query: 181 MC 183
           +C
Sbjct: 181 LC 180

[Close]

BLAST of Lag0033006 vs. NCBI nr
Match: XP_023534693.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 275.8 bits (704), Expect = 2.7e-70
Identity = 144/182 (79.12%), Postives = 158/182 (86.81%), Query Frame = 0


Query: 1   MRNHFSKSLLVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSK 60
           M+ HF  S+LVLV  SL TLFQFG SLDIV+RSCKLAA+ DPYVDYKLCV+SL+ANP+SK
Sbjct: 1   MKTHFPASILVLV--SLLTLFQFGFSLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSK 60

Query: 61  DATFKELVLISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKL 120
           DATFK+LVLISI+QSKANAT IG+EI+ LLKG GK GKYS SCL SCLE+YSEAVSDLKL
Sbjct: 61  DATFKDLVLISIEQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKL 120

Query: 121 ALAAVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIH 180
            L AVKM DYETANTEVSAAMDAP+TCE GY EKDGE SPLTE N GFFQL AISLAFI 
Sbjct: 121 GLVAVKMGDYETANTEVSAAMDAPLTCENGYNEKDGEVSPLTEKNGGFFQLTAISLAFIS 180

Query: 181 MC 183
           +C
Sbjct: 181 LC 180

[Close]

BLAST of Lag0033006 vs. NCBI nr
Match: KAG6606222.1 (hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036169.1 hypothetical protein SDJN02_02970, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 268.5 bits (685), Expect = 4.3e-68
Identity = 137/170 (80.59%), Postives = 151/170 (88.82%), Query Frame = 0


Query: 13  VLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDATFKELVLISI 72
           +LFSL TLFQFG  LDIV+RSCKLAA+ DPYVDYKLCV+SL+ANP+SKDATFK+LVLISI
Sbjct: 1   MLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVKSLRANPSSKDATFKDLVLISI 60

Query: 73  KQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKLALAAVKMADYET 132
           +QSKANAT IG+EI+ LLKG GK GKYS SCL SCLE+YSEAVSDLKL L AVKM DYET
Sbjct: 61  EQSKANATAIGSEITGLLKGGGKWGKYSTSCLESCLEVYSEAVSDLKLGLVAVKMGDYET 120

Query: 133 ANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIHMC 183
           ANTEVSAAMDAP+TCE+GY EKDGE SPLTE N GFFQL AISLAFI +C
Sbjct: 121 ANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFISLC 170

[Close]

BLAST of Lag0033006 vs. NCBI nr
Match: XP_038888305.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 251.9 bits (642), Expect = 4.2e-63
Identity = 130/173 (75.14%), Postives = 149/173 (86.13%), Query Frame = 0


Query: 11  VLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDATFKELVLI 70
           +L L  + TLFQ    LDIV+ SCKLAA+TDPYVDYKLCV+ LKANPNSKDA FK+LV+I
Sbjct: 9   ILFLLCVPTLFQLAYCLDIVQHSCKLAAKTDPYVDYKLCVQILKANPNSKDANFKDLVMI 68

Query: 71  SIKQSKANATGIGAEISELLKGSG-KLGKYSLSCLNSCLELYSEAVSDLKLALAAVKMAD 130
           SI QSKANAT I +EIS+L+KG+  K GKYSL+CL SCLELYSEAVSDLK AL A++M D
Sbjct: 69  SINQSKANATEIRSEISQLIKGTNEKWGKYSLNCLKSCLELYSEAVSDLKKALRALEMED 128

Query: 131 YETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIHMC 183
           YET NTEVSAAMDAPV+CE+GYKEKDGE SPLTE+NDGFFQL AISLAF++MC
Sbjct: 129 YETTNTEVSAAMDAPVSCEDGYKEKDGEVSPLTEINDGFFQLIAISLAFVNMC 181

[Close]

BLAST of Lag0033006 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-23
Identity = 69/168 (41.07%), Postives = 99/168 (58.93%), Query Frame = 0


Query: 10  LVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDATFKELVL 69
           L +  F+L  L Q   S DIV+ +CK  A+  P V+Y  CV+SL A+P S  A  + L +
Sbjct: 7   LCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGV 66

Query: 70  ISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKLALAAVKMAD 129
           IS   +  + + I   I  +LK   K+       LN C+ LY++A S ++ A+A  K  D
Sbjct: 67  ISANLAIQHGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKD 126

Query: 130 YETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLA 178
           Y +AN ++SAA+D  VTCE+G+KEK G  SP+T+ N  + QL AISLA
Sbjct: 127 YASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLA 172

[Close]

BLAST of Lag0033006 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.8e-23
Identity = 69/163 (42.33%), Postives = 96/163 (58.90%), Query Frame = 0


Query: 15  FSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDATFKELVLISIKQ 74
           FSL   F    S DIV+ +CK  A+  P V+Y  CV+SL A+P S  A  + L +IS   
Sbjct: 5   FSLCIFFLI--SADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANL 64

Query: 75  SKANATGIGAEISELLKGSGKLGKYSLSCLNSCLELYSEAVSDLKLALAAVKMADYETAN 134
           +    + I   I  +LK   K+       LN C+ LY++A S ++ A+A  K  DY +AN
Sbjct: 65  AIQQGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASAN 124

Query: 135 TEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLA 178
            ++SAA+D  VTCE+G+KEK G ASP+T+ N  + QL AISLA
Sbjct: 125 VKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLA 163

[Close]

BLAST of Lag0033006 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.0e-19
Identity = 69/178 (38.76%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 7   KSLLVLVLFSLQTLFQFGSSLDIVKRSCKLAARTDPYVDYKLCVRSLKANPNSKDA-TFK 66
           K L+ LV+FSL  L  F ++  +++ SCK A   DP + Y  CV SL  +P SK A T +
Sbjct: 2   KFLVSLVIFSL-FLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLE 61

Query: 67  ELVLISIKQSKANATGIGAEISELLKGSGKLGKYS--LSCLNSCLELYSEAVSDLKLALA 126
            LVL S K + A  T +   +++ LK      +Y   +  L  CL  Y++A  DL  ALA
Sbjct: 62  SLVLASTKTAAAKITNLKGIVAQDLKDQ----RYQDIVEDLKLCLGFYNDANDDLTTALA 121

Query: 127 AVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGFFQLAAISLAFIHM 182
            +K  DY+ AN  +SAA+D P  CE+ +KE   + SP+T  N   F+   I LAF +M
Sbjct: 122 NIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Lag0033006 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 2.7e-04
Identity = 35/122 (28.69%), Postives = 57/122 (46.72%), Query Frame = 0


Query: 49  CVRSLKANPNSKDATFKELVLISIKQSKANATGIGAEISELLKGSGKLGKYSLSCLNSCL 108
           C   +K+NP +  A  + L  I+   ++ +A+    +I  L+K +            SC+
Sbjct: 43  CTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTA--TNPTMKKAYTSCV 102

Query: 109 ELYSEAVSDLKLALAAVKMADYETANTEVSAAMDAPVTCEEGYKEKDGEASPLTEMNDGF 168
           + Y  A+S L  A  ++   D +  N +VSAAM+ P TCE+     D +  P    N G 
Sbjct: 103 QHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDM--ADFKVDPSAVKNSGD 160

Query: 169 FQ 171
           FQ
Sbjct: 163 FQ 160

The following BLAST results are available for this feature:

BLAST of Lag0033006 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958457.1	7.1e-71	79.12	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022995887.1	1.6e-70	79.12	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_023534693.1	2.7e-70	79.12	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
KAG6606222.1	4.3e-68	80.59	hypothetical protein SDJN03_03539, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038888305.1	4.2e-63	75.14	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of Lag0033006 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	1.5e-23	41.07	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	9.8e-23	42.33	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	3.0e-19	38.76	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	2.7e-04	28.69	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 25..178 e-value: 2.7E-30 score: 116.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..183 e-value: 2.7E-36 score: 122.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 29..177 e-value: 4.3E-29 score: 101.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 26..182 e-value: 4.0E-35 score: 123.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..181
None	No IPR available	PANTHER	PTHR31080:SF239	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 18..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 18..181
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 34..181 e-value: 5.99798E-46 score: 146.356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0033006.1	Lag0033006.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity