Lag0032845 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032845
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr11: 38164367 .. 38164855 (-)
RNA-Seq Expression	Lag0032845
Synteny	Lag0032845

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAGACCTTTCACCAAAGAGGAGATTGAAGCTGCTATCAAAGGAATGAACCCTACAAAGGCTTCGAGTCCTGACGGAGCCCACGCCAAGCTGTATCAGAAAGGTTGGGACACCCTAGGGGAAGACACCATTAAGGTCTGCTTAGGAATTCTAAACAATAGAGACAACATGGAAAACATCAACAACACATTGATAGCTCTCATCCCTAAAACTAAGGACCCGAAAAGGATGGCGGAATTCAGACCTATTAGCCTATGCAACGTGATCTACAAGGCAGTGGCCAAAGCAATAGCTAATAGAATGAAGACGGCCCTAGATACTATCATCTCCCCAAGTCAATCAGCATTCATCTCCGGCAGACAAATAACGGATAATGTGATAGTGGCGTTTGAATGCATCCACGCCTTGAACAAAAAAAGAAGGGGCAAAAAAGGGAACCTTGCCATAAAGCTTGACATGAGCAAAACTTATGACAGGGTGGAATGA

mRNA sequence

ATGGACAGACCTTTCACCAAAGAGGAGATTGAAGCTGCTATCAAAGGAATGAACCCTACAAAGGCTTCGAGTCCTGACGGAGCCCACGCCAAGCTGTATCAGAAAGGTTGGGACACCCTAGGGGAAGACACCATTAAGGTCTGCTTAGGAATTCTAAACAATAGAGACAACATGGAAAACATCAACAACACATTGATAGCTCTCATCCCTAAAACTAAGGACCCGAAAAGGATGGCGGAATTCAGACCTATTAGCCTATGCAACGTGATCTACAAGGCAGTGGCCAAAGCAATAGCTAATAGAATGAAGACGGCCCTAGATACTATCATCTCCCCAAGTCAATCAGCATTCATCTCCGGCAGACAAATAACGGATAATGTGATAGTGGCGTTTGAATGCATCCACGCCTTGAACAAAAAAAGAAGGGGCAAAAAAGGGAACCTTGCCATAAAGCTTGACATGAGCAAAACTTATGACAGGGTGGAATGA

Coding sequence (CDS)

ATGGACAGACCTTTCACCAAAGAGGAGATTGAAGCTGCTATCAAAGGAATGAACCCTACAAAGGCTTCGAGTCCTGACGGAGCCCACGCCAAGCTGTATCAGAAAGGTTGGGACACCCTAGGGGAAGACACCATTAAGGTCTGCTTAGGAATTCTAAACAATAGAGACAACATGGAAAACATCAACAACACATTGATAGCTCTCATCCCTAAAACTAAGGACCCGAAAAGGATGGCGGAATTCAGACCTATTAGCCTATGCAACGTGATCTACAAGGCAGTGGCCAAAGCAATAGCTAATAGAATGAAGACGGCCCTAGATACTATCATCTCCCCAAGTCAATCAGCATTCATCTCCGGCAGACAAATAACGGATAATGTGATAGTGGCGTTTGAATGCATCCACGCCTTGAACAAAAAAAGAAGGGGCAAAAAAGGGAACCTTGCCATAAAGCTTGACATGAGCAAAACTTATGACAGGGTGGAATGA

Protein sequence

MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMENINNTLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISGRQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE

Homology

[Close]

BLAST of Lag0032845 vs. NCBI nr
Match: CCA66036.1 (hypothetical protein [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 188.7 bits (478), Expect = 3.9e-44
Identity = 93/162 (57.41%), Postives = 113/162 (69.75%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           +D PF +EE+ AA+  M+P KA  PDG +A  YQ  WDT+GED     L +LNN DN+  
Sbjct: 433 LDAPFRREEVSAALAQMHPNKAPGPDGMNALFYQHFWDTIGEDVTTKVLNMLNNVDNIGA 492

Query: 61  INNTLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISG 120
           +N T I LIPK K  +   +FRPISLCNV+YK VAK +ANRMK  L  +I  SQS F+ G
Sbjct: 493 VNQTHIVLIPKKKHCESPVDFRPISLCNVLYKIVAKVLANRMKMVLPMVIHESQSGFVPG 552

Query: 121 RQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           R ITDNV+VA+EC H L KK+ GKKG L +KLDMSK YDRVE
Sbjct: 553 RLITDNVLVAYECFHFLRKKKTGKKGYLGLKLDMSKAYDRVE 594

[Close]

BLAST of Lag0032845 vs. NCBI nr
Match: XP_042962379.1 (uncharacterized protein LOC122296642 [Carya illinoinensis])

HSP 1 Score: 186.0 bits (471), Expect = 2.5e-43
Identity = 84/162 (51.85%), Postives = 123/162 (75.93%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           + +PFT+EE+ +A+  MNP  +  PDG  A  YQK W+ +GE+     L +LN   ++++
Sbjct: 233 LSKPFTREEVRSAVFQMNPLGSPGPDGFPAHFYQKHWEVVGEEVGSYVLEVLNCSRSLQD 292

Query: 61  INNTLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISG 120
           +N+T I+LIPK K+PKR+AEFRPISLCNV+YK V+K +ANRMK  L  +IS +QSAF+ G
Sbjct: 293 VNDTYISLIPKVKNPKRVAEFRPISLCNVLYKIVSKTLANRMKGVLHNLISLNQSAFVPG 352

Query: 121 RQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           R I+DN++VA+E +H++N + +GK+G +AIK+DMSK YDR+E
Sbjct: 353 RLISDNILVAYEVLHSMNSRMKGKRGGMAIKIDMSKAYDRIE 394

[Close]

BLAST of Lag0032845 vs. NCBI nr
Match: XP_030923330.1 (uncharacterized protein LOC115950239 [Quercus lobata])

HSP 1 Score: 185.3 bits (469), Expect = 4.3e-43
Identity = 87/162 (53.70%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           +   FT EE++AA+  M PTKA  PDG +A  YQK W  +G+  +   L  LNN + +  
Sbjct: 825 LSNQFTAEEVQAALFQMGPTKAPGPDGMNALFYQKFWHIVGDSVVSAVLDFLNNGNMLPE 884

Query: 61  INNTLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISG 120
           IN+T I LIPK ++P+RM+EFRPISLCNVIYK ++K +ANR+K  L  IIS +QSAF+ G
Sbjct: 885 INHTNIVLIPKVQNPERMSEFRPISLCNVIYKIISKVLANRLKQVLPQIISSTQSAFVPG 944

Query: 121 RQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           R ITDNV+VA+E +H ++ +++GKKG++A+KLD+SK YDRVE
Sbjct: 945 RLITDNVLVAYETLHTMHARKKGKKGDVALKLDISKAYDRVE 986

[Close]

BLAST of Lag0032845 vs. NCBI nr
Match: KAF5471784.1 (hypothetical protein F2P56_008554 [Juglans regia])

HSP 1 Score: 184.9 bits (468), Expect = 5.6e-43
Identity = 85/159 (53.46%), Postives = 120/159 (75.47%), Query Frame = 0


Query: 4   PFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMENINN 63
           PFT+EEI AA+  MNP  +  PDG  A+ YQK W+ +GE      L  LN+  ++ ++N+
Sbjct: 377 PFTREEINAAVFHMNPLGSPGPDGFPAQFYQKHWEVVGEQVCSYALNFLNHGGSLNDVND 436

Query: 64  TLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISGRQI 123
           T I+LIPK + PK++A++RPISLCNV+YK V+K IANR+K  L  II+P+QSAF++GR I
Sbjct: 437 TYISLIPKVQSPKKVADYRPISLCNVLYKIVSKTIANRLKKILPQIIAPNQSAFVAGRLI 496

Query: 124 TDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           +DN +VA+E +H++N + +GKKG +A+KLDMSK YDRVE
Sbjct: 497 SDNTMVAYEILHSMNSRMQGKKGFMAVKLDMSKAYDRVE 535

[Close]

BLAST of Lag0032845 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 184.1 bits (466), Expect = 9.5e-43
Identity = 90/158 (56.96%), Postives = 117/158 (74.05%), Query Frame = 0


Query: 5   FTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMENINNT 64
           FT+EEIE A+  M+PTKA  PDG  A  +QK W+ +G D + + L +LN+  +M  IN T
Sbjct: 268 FTREEIETALNQMHPTKAPGPDGMSAIFFQKYWNIVGNDIVCMVLDVLNSNMSMVEINKT 327

Query: 65  LIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISGRQIT 124
            I L+PK K+P +M++FRPISLCNV+YK ++K +ANR+K  L  IIS +QSAF+SGR IT
Sbjct: 328 NITLVPKIKNPTKMSDFRPISLCNVVYKLISKVLANRLKNILPQIISENQSAFLSGRLIT 387

Query: 125 DNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           DNV+VAFE +H L  K+ GK+G  AIKLDMSK YDRVE
Sbjct: 388 DNVLVAFELMHYLEHKKEGKEGFAAIKLDMSKAYDRVE 425

[Close]

BLAST of Lag0032845 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.5e-17
Identity = 53/162 (32.72%), Postives = 85/162 (52.47%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           ++ P T +E+  A++ M   K+   DG   + +Q  WDTLG D  +V        +   +
Sbjct: 445 LETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLS 504

Query: 61  INNTLIALIPKTKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFISG 120
               +++L+PK  D + +  +RP+SL +  YK VAKAI+ R+K+ L  +I P QS  + G
Sbjct: 505 CRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPG 564

Query: 121 RQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           R I DNV +  + +H     RR       + LD  K +DRV+
Sbjct: 565 RTIFDNVFLIRDLLHF---ARRTGLSLAFLSLDQEKAFDRVD 603

[Close]

BLAST of Lag0032845 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 7.1e-17
Identity = 57/167 (34.13%), Postives = 88/167 (52.69%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           ++ P + +EIEA I  +   K+  PDG  A+ YQ    T  ED I +   + +  +    
Sbjct: 456 LNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQ----TFKEDLIPILHKLFHKIEVEGT 515

Query: 61  INNTL----IALIPK-TKDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQS 120
           + N+     I LIPK  KDP ++  FRPISL N+  K + K +ANR++  +  II P Q 
Sbjct: 516 LPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQV 575

Query: 121 AFISGRQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
            FI G Q   N+  +   IH +NK +   K ++ I LD  K +D+++
Sbjct: 576 GFIPGMQGWFNIRKSINVIHYINKLK--DKNHMIISLDAEKAFDKIQ 616

[Close]

BLAST of Lag0032845 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-14
Identity = 51/167 (30.54%), Postives = 84/167 (50.30%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           ++RP +  EI + I+ +   K+  PDG  ++ YQ    T  E+ + + L +  N +    
Sbjct: 448 LNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQ----TFKEELVPILLNLFQNIEKEGI 507

Query: 61  INNTL----IALIPKT-KDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQS 120
           + NT     I LIPK  KDP R   +RPISL N+  K + K + NR++  +  II   Q 
Sbjct: 508 LPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQV 567

Query: 121 AFISGRQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
            FI G Q   N+  +   I  +NK +   K ++ + +D  K +D ++
Sbjct: 568 GFIPGSQGWFNIRKSINVIQHINKLK--NKDHMILSIDAEKAFDNIQ 608

[Close]

BLAST of Lag0032845 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.0e-12
Identity = 49/163 (30.06%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 1   MDRPFTKEEIEAAIKGMNPTKASSPDGAHAKLYQKGWDTLGEDTIKVCLGILNNRDNMEN 60
           ++RP T  EI A I  +   K+  PDG  A+ YQ+  + L    +K+   I        +
Sbjct: 449 LNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNS 508

Query: 61  INNTLIALIPKT-KDPKRMAEFRPISLCNVIYKAVAKAIANRMKTALDTIISPSQSAFIS 120
                I LIPK  +D  +   FRPISL N+  K + K +ANR++  +  +I   Q  FI 
Sbjct: 509 FYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIHHDQVGFIP 568

Query: 121 GRQITDNVIVAFECIHALNKKRRGKKGNLAIKLDMSKTYDRVE 163
           G Q   N+  +   I  +N+ +   K ++ I +D  K +D+++
Sbjct: 569 GMQGWFNIRKSINVIQHINRAK--DKNHVIISIDAEKAFDKIQ 609

The following BLAST results are available for this feature:

BLAST of Lag0032845 vs. NCBI nr


Match Name	E-value	Identity	Description
CCA66036.1	3.9e-44	57.41	hypothetical protein [Beta vulgaris subsp. vulgaris]	[more]
XP_042962379.1	2.5e-43	51.85	uncharacterized protein LOC122296642 [Carya illinoinensis]	[more]
XP_030923330.1	4.3e-43	53.70	uncharacterized protein LOC115950239 [Quercus lobata]	[more]
KAF5471784.1	5.6e-43	53.46	hypothetical protein F2P56_008554 [Juglans regia]	[more]
XP_023881891.1	9.5e-43	56.96	uncharacterized protein LOC111994244 [Quercus suber]	[more]

BLAST of Lag0032845 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14381	2.5e-17	32.72	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]
P11369	7.1e-17	34.13	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
P08548	3.3e-14	30.54	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
O00370	4.0e-12	30.06	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 70..162 e-value: 1.3E-16 score: 60.8
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 3..162
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 3..162
None	No IPR available	CDD	cd01650	RT_nLTR_like	coord: 64..162 e-value: 1.90484E-30 score: 108.147
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 8..161

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032845.1	Lag0032845.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid