Lag0032475 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032475
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrotransposon protein
Locationchr11: 33031504 .. 33031887 (-)
RNA-Seq ExpressionLag0032475
SyntenyLag0032475
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAATTCATATTCATCTTTCTTGGGTGGGAATGCACATTGGACTAATGATTATGGCCTATCTACAAGTTACTATTATTTTTGCGACATCGGTTATCCAAATGTAGAGGGATTTATGGCATCTTACCGAGGTCAACGATACCACCTTACAGAATGGCATGGACGGAACCCACTGACCAACCTGAAAGAGTTATTCAACATGAGACATTCCATTGCTGAAAAAATGATTGAACGAGTGTTTGGTATGTTGAAGGGTCGGTGGGCAATCTTACGAGGCAATTCCTACTATCCCGTTGATATGCAATGTAGGTTGATCATTGCTTGTTGTTTGCTCCATAACTTGATCATTCGAGAAATGGGATTGTTGCCTTGTGCAATATAA

mRNA sequence

ATGACGAATTCATATTCATCTTTCTTGGGTGGGAATGCACATTGGACTAATGATTATGGCCTATCTACAAGTTACTATTATTTTTGCGACATCGGTTATCCAAATGTAGAGGGATTTATGGCATCTTACCGAGGTCAACGATACCACCTTACAGAATGGCATGGACGGAACCCACTGACCAACCTGAAAGAGTTATTCAACATGAGACATTCCATTGCTGAAAAAATGATTGAACGAGTGTTTGGTATGTTGAAGGGTCGGTGGGCAATCTTACGAGGCAATTCCTACTATCCCGTTGATATGCAATGTAGGTTGATCATTGCTTGTTGTTTGCTCCATAACTTGATCATTCGAGAAATGGGATTGTTGCCTTGTGCAATATAA

Coding sequence (CDS)

ATGACGAATTCATATTCATCTTTCTTGGGTGGGAATGCACATTGGACTAATGATTATGGCCTATCTACAAGTTACTATTATTTTTGCGACATCGGTTATCCAAATGTAGAGGGATTTATGGCATCTTACCGAGGTCAACGATACCACCTTACAGAATGGCATGGACGGAACCCACTGACCAACCTGAAAGAGTTATTCAACATGAGACATTCCATTGCTGAAAAAATGATTGAACGAGTGTTTGGTATGTTGAAGGGTCGGTGGGCAATCTTACGAGGCAATTCCTACTATCCCGTTGATATGCAATGTAGGTTGATCATTGCTTGTTGTTTGCTCCATAACTTGATCATTCGAGAAATGGGATTGTTGCCTTGTGCAATATAA

Protein sequence

MTNSYSSFLGGNAHWTNDYGLSTSYYYFCDIGYPNVEGFMASYRGQRYHLTEWHGRNPLTNLKELFNMRHSIAEKMIERVFGMLKGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREMGLLPCAI
Homology
BLAST of Lag0032475 vs. NCBI nr
Match: XP_008455792.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 154.1 bits (388), Expect = 8.3e-34
Identity = 70/105 (66.67%), Postives = 80/105 (76.19%), Query Frame = 0

Query: 17  NDYGLSTSYYYFCDIGYPNVEGFMASYRGQRYHLTEWHGR-NPLTNLKELFNMRHSIAEK 76
           N   +   YYY CD GYPN EGF+A YRG+RYHL+EW G  N  T  +E FNM+HS A  
Sbjct: 165 NGLKVPKGYYYLCDAGYPNAEGFLAPYRGERYHLSEWRGESNAPTTAREFFNMKHSSARN 224

Query: 77  MIERVFGMLKGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREM 121
           +IER FG+LKGRWAILRG SYYPVD+QCR I+ACCLLHNLI REM
Sbjct: 225 VIERAFGLLKGRWAILRGKSYYPVDVQCRTIMACCLLHNLINREM 269

BLAST of Lag0032475 vs. NCBI nr
Match: KAA0048281.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 153.7 bits (387), Expect = 1.1e-33
Identity = 71/105 (67.62%), Postives = 80/105 (76.19%), Query Frame = 0

Query: 17  NDYGLSTSYYYFCDIGYPNVEGFMASYRGQRYHLTEWHG-RNPLTNLKELFNMRHSIAEK 76
           N   +   YYY  D GYPN EGF+A YRGQRYHL EWHG  N  + LKE FNM+HS A  
Sbjct: 165 NGLKVPKGYYYLVDAGYPNAEGFLAPYRGQRYHLQEWHGPENAPSTLKEFFNMKHSSARN 224

Query: 77  MIERVFGMLKGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREM 121
           +IER FG+LKGRWAILRG SYYPV++QCR I+ACCLLHNLI REM
Sbjct: 225 VIERTFGVLKGRWAILRGKSYYPVEVQCRTILACCLLHNLINREM 269

BLAST of Lag0032475 vs. NCBI nr
Match: KAA0065306.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.9 bits (385), Expect = 1.8e-33
Identity = 69/97 (71.13%), Postives = 75/97 (77.32%), Query Frame = 0

Query: 25  YYYFCDIGYPNVEGFMASYRGQRYHLTEWHGRNPLTNLKELFNMRHSIAEKMIERVFGML 84
           YYY CD GYPN EGF+A YRGQRYHLTEW G NP    KELFNMRHS A  +IER FG L
Sbjct: 169 YYYLCDAGYPNAEGFLAPYRGQRYHLTEWRGGNPPKCPKELFNMRHSFARNVIERAFGSL 228

Query: 85  KGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREMG 122
             RW IL+G SYY VD+QC++I ACCLLHNLI REMG
Sbjct: 229 NCRWTILQGRSYYLVDIQCKIITACCLLHNLIHREMG 265

BLAST of Lag0032475 vs. NCBI nr
Match: KAA0060719.1 (retrotransposon protein [Cucumis melo var. makuwa] >TYK01462.1 retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 2.4e-33
Identity = 71/101 (70.30%), Postives = 78/101 (77.23%), Query Frame = 0

Query: 21  LSTSYYYFCDIGYPNVEGFMASYRGQRYHLTEWHG-RNPLTNLKELFNMRHSIAEKMIER 80
           L T YYY CD GYPN EGF+A YRGQRYHL EW G  N  TN KE FNM+HS A  +IER
Sbjct: 11  LITGYYYLCDTGYPNAEGFLAPYRGQRYHLQEWRGAANAPTNAKEYFNMKHSSARNVIER 70

Query: 81  VFGMLKGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREM 121
            FG+LKGRWAILRG SYYP+ +QCR I+AC LLHNLI REM
Sbjct: 71  SFGVLKGRWAILRGKSYYPLQVQCRTILACALLHNLINREM 111

BLAST of Lag0032475 vs. NCBI nr
Match: KAA0048191.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 3.1e-33
Identity = 70/96 (72.92%), Postives = 75/96 (78.12%), Query Frame = 0

Query: 25  YYYFCDIGYPNVEGFMASYRGQRYHLTEWHGRNPLTNLKELFNMRHSIAEKMIERVFGML 84
           YYY CD  Y NVEGF+A YRGQRYHL EW G NP    KELFNMRHS A  +IER FG L
Sbjct: 182 YYYLCDASYLNVEGFLAPYRGQRYHLIEWRGGNPPKCPKELFNMRHSSARNVIERAFGSL 241

Query: 85  KGRWAILRGNSYYPVDMQCRLIIACCLLHNLIIREM 121
           K RWAILRG SYYPVD+QC++I ACCLLHNLI REM
Sbjct: 242 KSRWAILRGRSYYPVDIQCKIITACCLLHNLIHREM 277

BLAST of Lag0032475 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-05
Identity = 31/93 (33.33%), Postives = 44/93 (47.31%), Query Frame = 0

Query: 26  YYFCDIGYPNVEGFMASYRGQRYHLTEWHGRNPLTNLKELFNMRHSIAEKMIERVFGMLK 85
           Y   D G+P +   +  Y+G+           P +  +  FN RHS A K  +     LK
Sbjct: 275 YIVGDSGFPLLPWLLTPYQGK-----------PTSLPQTEFNKRHSEATKAAQMALSKLK 334

Query: 86  GRWAILRGNSYYPVDMQC-RLIIACCLLHNLII 118
            RW I+ G  + P   +  R+I  CCLLHN+II
Sbjct: 335 DRWRIINGVMWMPDRNRLPRIIFVCCLLHNIII 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455792.18.3e-3466.67PREDICTED: putative nuclease HARBI1 [Cucumis melo][more]
KAA0048281.11.1e-3367.62retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0065306.11.8e-3371.13retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0060719.12.4e-3370.30retrotransposon protein [Cucumis melo var. makuwa] >TYK01462.1 retrotransposon p... [more]
KAA0048191.13.1e-3372.92retrotransposon protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9M2U31.3e-0533.33Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 23..114
e-value: 4.9E-12
score: 45.8
NoneNo IPR availablePANTHERPTHR22930:SF218DUAL SPECIFICITY PHOSPHATASE DOMAIN PROTEINcoord: 22..121
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 22..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032475.1Lag0032475.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding