Lag0031968 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031968 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 795.8 bits (2054), Expect = 2.1e-226
Identity = 389/490 (79.39%), Postives = 431/490 (87.96%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S SFL   L+++PL FIILRHI  +SKQ P PPGP+PWPILGNLLQIGQ+AHISMT+F+ 
Sbjct: 5   SFSFL---LLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSH 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
           IYGPL+SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLH++CSL+FSPE
Sbjct: 65  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  RWK LRSICRA LFT RAIE  SQA+LRR KMN+M++FL SKQGS VEIK+ VF TV
Sbjct: 125 CGDRWKNLRSICRAKLFTARAIE--SQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSNFIFS DL+ FVGDGFNGIK PFKNM+DLGL PNLADFYPLLR FDLQGLRKKAA
Sbjct: 185 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY++QV+S WEILI+ERR IHSQ   AA DFLD+LIQSGFTD+QI YLIVEVLSAGTDTT
Sbjct: 245 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAP 368
           TTTVEW MAELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAP
Sbjct: 305 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 364

Query: 369 FLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYD 428
           FLLPR APEACELMGYS+ K T+IFVNVW IGRDPS+WEDP SF P+RF GCD D KG+D
Sbjct: 365 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 424

Query: 429 FRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQ 488
           +R+LPFGGGRRICPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD NGRLGVTLQK+
Sbjct: 425 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 484

Query: 489 EPLKLIPKRR 497
           EPLKL+ K R
Sbjct: 485 EPLKLLSKNR 489

BLAST of Lag0031968 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 784.3 bits (2024), Expect = 6.4e-223
Identity = 384/490 (78.37%), Postives = 429/490 (87.55%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S SFL   L+++PL F ILRHI  +SKQ P PPGP PWPILGNLLQIGQ+AHISMT+F+ 
Sbjct: 5   SFSFL---LLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSH 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
            YGPL+SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLH++CSL+FSPE
Sbjct: 65  FYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  RWK LRSICRA LFT RAIE  SQA+LRR KMNDM++FL SKQGS VEIK+ VF TV
Sbjct: 125 CGDRWKNLRSICRAKLFTARAIE--SQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSNFIFS DL+ FVGDGFNGIK  FKN++DLGL PNLADFYPLLR+FDLQGLRKKAA
Sbjct: 185 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY++QV+S WEILI+ERR IHS+   AA DFLD+LI+SGFTD+QI YLIVEVLSAGTDTT
Sbjct: 245 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAP 368
           TTTVEW MAELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAP
Sbjct: 305 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 364

Query: 369 FLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYD 428
           FLLPR APEACELMGYS+ KDT+IFVNVW IGRDPS+WEDP SF P+RF G D D KG+D
Sbjct: 365 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 424

Query: 429 FRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQ 488
           +R+LPFGGGRRICPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD NGRLGVTLQK+
Sbjct: 425 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 484

Query: 489 EPLKLIPKRR 497
           EPLKL+ K+R
Sbjct: 485 EPLKLLSKKR 489

BLAST of Lag0031968 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 768.5 bits (1983), Expect = 3.6e-218
Identity = 375/490 (76.53%), Postives = 427/490 (87.14%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S SFLL  L+++PL   IL+HI  +SKQ P PPGP+PWP+LGNLLQIGQ+AHISMT+F+ 
Sbjct: 5   SFSFLL--LLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSH 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
           IYGPL+SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LH++CSLVFSPE
Sbjct: 65  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  RWK LRSICRA LFT RAIE  SQA+LRR KMN+M++FL SKQGS VEIK+FVF TV
Sbjct: 125 CGDRWKNLRSICRAKLFTARAIE--SQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSNFIFS DL+ FVGDGFNGIK  FKN++DL L PNLADFYPLLR FDLQGLRKKAA
Sbjct: 185 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY++Q++S WEILI+ERR IHS+   AA DFLD+LIQSG+T++QI YLIVEVLSAGTD  
Sbjct: 245 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAP 368
           TTTVEW MAELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAP
Sbjct: 305 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 364

Query: 369 FLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYD 428
           FLLPR APEACELMGYS+ K T+IFVNVW IGRDPS+WEDP SF P+RF GCD D KG+D
Sbjct: 365 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 424

Query: 429 FRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQ 488
           +R+LPFGGGRR+CPG+PMAAVQ+PLILAT IHNFEW LPN E+ S LD NGRLG+TLQK+
Sbjct: 425 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 484

Query: 489 EPLKLIPKRR 497
           EPLKL+PK+R
Sbjct: 485 EPLKLLPKKR 488

BLAST of Lag0031968 vs. NCBI nr
Match: XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 768.1 bits (1982), Expect = 4.7e-218
Identity = 371/483 (76.81%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 17  LVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSL 76
           L+++PL F IL+HI  +SKQ P PPGP+PWP+LGNLLQIGQ+AHISMT+F+ IYGPL+SL
Sbjct: 9   LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISL 68

Query: 77  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRWKTL 136
           KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LH++CSLVFSPEC  RWK L
Sbjct: 69  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNL 128

Query: 137 RSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLSNFI 196
           RSICRA LFT RAIE  SQA+LRR KMN+M++FL SKQGS VEIK+FVF TVFNTLSNFI
Sbjct: 129 RSICRAKLFTARAIE--SQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 188

Query: 197 FSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQVNS 256
           FS DL+ FVGDGFNGI+  FKN++D  L PNLADFYPLLR FDLQGLRKKAAIY++Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 248

Query: 257 CWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTTVEWTM 316
            WEILI+ERR IHS+      DFLD+LIQSGFTD+QI YLIVEVLSAGTDTTTTTVEW M
Sbjct: 249 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 308

Query: 317 AELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPRIAP 376
           AELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 377 EACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLPFGG 436
           EACELMGYS+ K T+I VNVW IGRDPS+WEDP SF P+RF  CD D KG+D+R+LPFGG
Sbjct: 369 EACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFGG 428

Query: 437 GRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKLIPK 496
           GRR+CPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD  GRLGVTLQK+EPLKL+PK
Sbjct: 429 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLPK 488

Query: 497 RRM 498
           RR+
Sbjct: 489 RRI 489

BLAST of Lag0031968 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764.6 bits (1973), Expect = 5.2e-217
Identity = 368/482 (76.35%), Postives = 420/482 (87.14%), Query Frame = 0

Query: 17  LVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSL 76
           L+++PL F +L+HI  +SKQ P PPGP PWPILGNLLQIGQ+AHISMT+F+ IYGPL+SL
Sbjct: 9   LLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIYGPLISL 68

Query: 77  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRWKTL 136
           KLGAQRLVVASSPAAAAAILKTQDRLLSARYI QMTPDR LH++CSL+FSPEC  RWK L
Sbjct: 69  KLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECGDRWKNL 128

Query: 137 RSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLSNFI 196
           RSICRA LFT RAIE  SQA+LRR KM DM++FL SKQGS VEIK+FVF TVFNTLSN I
Sbjct: 129 RSICRAKLFTARAIE--SQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITVFNTLSNLI 188

Query: 197 FSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQVNS 256
           FS DL+ FVGDGFNGIK PFKNM+DLGL PNLADFYPLLR+FDLQGLRKKAAIY++Q++S
Sbjct: 189 FSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQIDS 248

Query: 257 CWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTTVEWTM 316
             EILI+ERR IHS+   AA DFLDILI+SGFT++QI YLI+EVL AGTDTTTTTVEW M
Sbjct: 249 SSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTTTTTVEWAM 308

Query: 317 AELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPRIAP 376
           AEL+KN+DIMN+VREE K+ +NG  +DES + +LHYLW CVKET+RLHPPAPFLLPR AP
Sbjct: 309 AELMKNRDIMNRVREETKKALNGGAIDESKLSELHYLWKCVKETVRLHPPAPFLLPRYAP 368

Query: 377 EACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLPFGG 436
           EACELMGYS+ K T+IFVNVW IGRDPS+WEDP SF P+RF   D D KG+D+R+LPFGG
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHDYRFLPFGG 428

Query: 437 GRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKLIPK 496
           GRR CPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD NGRLG+TLQK+EPLKL+PK
Sbjct: 429 GRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKEEPLKLVPK 488

BLAST of Lag0031968 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.1e-122
Identity = 233/499 (46.69%), Postives = 321/499 (64.33%), Query Frame = 0

Query: 3   MDILSL---SSSFLLPALVVVPLAFIILRHINSKQAPNPPGPRPWPILGNLLQIGQSAHI 62
           M++LS+   S SFLL         F ILR  +S+    PPGPRP PI+GNLLQ+G   H 
Sbjct: 1   MEVLSIAIVSFSFLL-------FLFFILR--DSRPKNLPPGPRPSPIVGNLLQLGDKPHA 60

Query: 63  SMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHC 122
              + AQ YG L SLKLG+Q +VVASSPAAAA ILKT D++LS RY+FQ    +  H   
Sbjct: 61  EFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVEN 120

Query: 123 SLVFSPECNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIK 182
           S+V+S ECN  WK LR +CR  LFT + IES S+  +R AK  +M++FL  K+G  V+I 
Sbjct: 121 SIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSE--IREAKAREMVKFLRGKEGEVVKIV 180

Query: 183 DFVFTTVFNTLSNFIFSTDL--LSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFD 242
           + VF T+ N   N IFS D+  L     G   +K     ++D+G + N AD++P++   D
Sbjct: 181 EVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLD 240

Query: 243 LQGLRKKAAIYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVE 302
           L G R+  A    Q+   W ++++ERR   ++ + +  DF+D+L+ +G  D +I  L++E
Sbjct: 241 LFGQRRAVAEVLQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLME 300

Query: 303 VLSAGTDTTTTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKE 362
           +  AGT+T+ +T+EW + EL K   +++K+R E+  V+  +TV ESD+P L YL   VKE
Sbjct: 301 LFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKE 360

Query: 363 TLRLHPPAPFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVG 422
           TLRLHPP P LLPR A E C +M Y+I K+  I VN WAIGRDP  W+DPL+F P+RF+ 
Sbjct: 361 TLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLS 420

Query: 423 CDVDFKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNG 482
            DVD+KG DF  +PFGGGRRICPG+P+A+    LI+AT + NFEWSLP G   S+L  + 
Sbjct: 421 SDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDE 480

Query: 483 RLGVTLQKQEPLKLIPKRR 497
           + G+TLQK  PL ++ K R
Sbjct: 481 KFGLTLQKDPPLLIVLKAR 484

BLAST of Lag0031968 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.1e-116
Identity = 216/493 (43.81%), Postives = 309/493 (62.68%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHINSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIY 68
           +S+  L A+++  + +++      K    PPGP+PWPI+GNLLQ+G+  H      AQ Y
Sbjct: 2   TSTVALIAVIISSILYLLFGGSGHKNL--PPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 61

Query: 69  GPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECN 128
           G + +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C 
Sbjct: 62  GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCT 121

Query: 129 GRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFN 188
             WK LR +CR  LFT + IE  SQA +R  K  +M+++L  KQG  V+I + +F T+ N
Sbjct: 122 ETWKNLRKVCRTELFTQKMIE--SQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 181

Query: 189 TLSNFIFSTDLLSFVGD---GFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKA 248
              N IFS ++   +GD   G +  K     M++LG + N AD++P+L  FDL G RK+ 
Sbjct: 182 IFGNLIFSQNIFE-LGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEV 241

Query: 249 AIYRDQVNSCWEILIQERRKIHS-QSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTD 308
           A     + + W  ++QER+         +  DF+D+ + SG  D QI  L++E+  AGT+
Sbjct: 242 AECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTE 301

Query: 309 TTTTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPP 368
           T+ +T+EW M EL KN  I  K+R E++ V+   +V ESD P L YL   VKETLRLHPP
Sbjct: 302 TSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPP 361

Query: 369 APFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKG 428
            P LLPR A E C ++ Y+I KD  I VN W IGRDP  W DPL+F+P+RF+   VDF+G
Sbjct: 362 TPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRG 421

Query: 429 YDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQ 488
            DF  +PFG GRRICPG+P+A   + L++ATF+ N +W LPNG  +  L    + G+TLQ
Sbjct: 422 NDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQ 481

Query: 489 KQEPLKLIPKRRM 498
           K+ PL ++PK R+
Sbjct: 482 KEPPLFIVPKSRV 487

BLAST of Lag0031968 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.4e-116
Identity = 217/486 (44.65%), Postives = 304/486 (62.55%), Query Frame = 0

Query: 16  ALVVVPLAFIILRHINSKQAPN-PPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSL 75
           AL+ V ++ I+     S    N PPGP+PWPI+GNLLQ+G+  H      AQ YG + +L
Sbjct: 7   ALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 66

Query: 76  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRWKTL 135
           K+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C   WK L
Sbjct: 67  KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNL 126

Query: 136 RSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLSNFI 195
           R +CR  LFT + IE  SQA +R  K  +M+++L  KQG  V+I + +F T+ N   N I
Sbjct: 127 RKVCRTELFTQKMIE--SQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLI 186

Query: 196 FSTDL--LSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQV 255
           FS ++  L     G +  K     M++LG + N AD++P+L  FDL G RK+ A     +
Sbjct: 187 FSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGI 246

Query: 256 NSCWEILIQERRKIHS-QSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTTVE 315
            + W  ++QER+         +  DF+D+ + SG  D QI  L++E+  AGT+T+ +T+E
Sbjct: 247 YAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIE 306

Query: 316 WTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPR 375
           W M EL KN  I  K+R E++ V+   +V ESD P L YL   VKETLRLHPP P LLPR
Sbjct: 307 WAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPR 366

Query: 376 IAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLP 435
            A E C ++ Y+I KD  I VN W IGRDP  W DPL+F+P+RF+   VDF+G DF  +P
Sbjct: 367 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIP 426

Query: 436 FGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKL 495
           FG GRRICPG+P+A   + L++ATF+ N +W LPNG  +  L    + G+TLQK+ PL +
Sbjct: 427 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 486

Query: 496 IPKRRM 498
           +PK R+
Sbjct: 487 VPKSRV 488

BLAST of Lag0031968 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.9e-116
Identity = 215/486 (44.24%), Postives = 305/486 (62.76%), Query Frame = 0

Query: 14  LPALVVVPLAFIILRHINSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVS 73
           L A+V+    ++I R  + K    PPGP+PWPI+GNLLQ+G+  H    + A+ YG L S
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGL--PPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFS 61

Query: 74  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRWKT 133
           LKLG++ +VVAS+P AA+ ILKT DR+LS RY+FQ    +  H   S+V+S ECN  WK 
Sbjct: 62  LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKK 121

Query: 134 LRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLSNF 193
           LR +CR  LFT + IE  SQA +R +K  +M+++L    G+ V+I + VF T+ N   N 
Sbjct: 122 LRKVCRTELFTQKMIE--SQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNL 181

Query: 194 IFSTDLLSFVGDGFNG---IKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRD 253
           IFS ++   +GD  +G   +K     M++LG + N AD++P L  FDL G  K  A    
Sbjct: 182 IFSQNIFK-LGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQ 241

Query: 254 QVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTTV 313
            + S W  +++ER+     +     DF++IL+ SG  D QI  L++E+  AGT+T+ +T+
Sbjct: 242 GIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTI 301

Query: 314 EWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLP 373
           EW ++EL KN  +   +R E+  V+    V ESDIP + YL   VKETLRLHP  P LLP
Sbjct: 302 EWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLP 361

Query: 374 RIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYL 433
           R A E C+++ Y+I K+  I VN W IGRDP  W DPL F+P+RF+   +DFKG DF  +
Sbjct: 362 RRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELI 421

Query: 434 PFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLK 493
           PFG GRRICPG+P+A   + LI+++ + NF+W  P G D S+L    + G+TLQK+ PL 
Sbjct: 422 PFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLY 480

Query: 494 LIPKRR 497
           ++PK R
Sbjct: 482 IVPKTR 480

BLAST of Lag0031968 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 6.6e-106
Identity = 206/487 (42.30%), Postives = 306/487 (62.83%), Query Frame = 0

Query: 16  ALVVVPLAFIILRHINSKQAPNPPGPRPWPILGNLLQIGQSA----HISMTRFAQIYGPL 75
           +L+ V L FI+L  +  K    PPGP PWP++GNL  +  +     HI++   A+ +GP+
Sbjct: 9   SLIPVILVFILL--LKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPI 68

Query: 76  VSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRW 135
           + L LG Q  V+AS+  AA  ILKT DR+ SAR+I +M+     H   SLV+S +C   W
Sbjct: 69  MILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS-DCTDYW 128

Query: 136 KTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLS 195
           K LR I R  +F+ + ++  +Q+ +R  K+ +++ FL SK+G  V+I  FVF T+ N L 
Sbjct: 129 KLLRKIVRTEIFSPKMLQ--AQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILG 188

Query: 196 NFIFSTDLLSFVGDGFN--GIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYR 255
           N +FS D+  + GD  +  GI+   + M+ +G  PN+A+FYP L   DLQGL+KK     
Sbjct: 189 NVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERF 248

Query: 256 DQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTT 315
            +V   WE  ++ER+   ++    + D LD+L+ + F D QI  L +E    G++T++ T
Sbjct: 249 IRVMKMWEGTVKERKANRNEE---SKDMLDVLLANDFNDAQINALFLETFGPGSETSSAT 308

Query: 316 VEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLL 375
           +EW +AEL+K+   M KVR+E+ EV+  ST+ ESD+PQL YL  C+KE +RLHP APFLL
Sbjct: 309 IEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLL 368

Query: 376 PRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRY 435
           PR A E CE+MGY+I K++ + VN +AIGRDP  W+DP +F P+RF+  DVDF G  +++
Sbjct: 369 PRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQF 428

Query: 436 LPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPL 495
           +PFG GRR C G+P+A   +PLI+ + +HN+++ LP G     L  N  L +TL     L
Sbjct: 429 IPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSL 485

Query: 496 KLIPKRR 497
            ++PK R
Sbjct: 489 CVVPKAR 485

BLAST of Lag0031968 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 1.0e-226
Identity = 389/490 (79.39%), Postives = 431/490 (87.96%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S SFL   L+++PL FIILRHI  +SKQ P PPGP+PWPILGNLLQIGQ+AHISMT+F+ 
Sbjct: 5   SFSFL---LLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSH 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
           IYGPL+SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLH++CSL+FSPE
Sbjct: 65  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  RWK LRSICRA LFT RAIE  SQA+LRR KMN+M++FL SKQGS VEIK+ VF TV
Sbjct: 125 CGDRWKNLRSICRAKLFTARAIE--SQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSNFIFS DL+ FVGDGFNGIK PFKNM+DLGL PNLADFYPLLR FDLQGLRKKAA
Sbjct: 185 FNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAA 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY++QV+S WEILI+ERR IHSQ   AA DFLD+LIQSGFTD+QI YLIVEVLSAGTDTT
Sbjct: 245 IYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAP 368
           TTTVEW MAELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAP
Sbjct: 305 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 364

Query: 369 FLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYD 428
           FLLPR APEACELMGYS+ K T+IFVNVW IGRDPS+WEDP SF P+RF GCD D KG+D
Sbjct: 365 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 424

Query: 429 FRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQ 488
           +R+LPFGGGRRICPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD NGRLGVTLQK+
Sbjct: 425 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKE 484

Query: 489 EPLKLIPKRR 497
           EPLKL+ K R
Sbjct: 485 EPLKLLSKNR 489

BLAST of Lag0031968 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 3.1e-223
Identity = 384/490 (78.37%), Postives = 429/490 (87.55%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S SFL   L+++PL F ILRHI  +SKQ P PPGP PWPILGNLLQIGQ+AHISMT+F+ 
Sbjct: 5   SFSFL---LLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSH 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
            YGPL+SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLH++CSL+FSPE
Sbjct: 65  FYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  RWK LRSICRA LFT RAIE  SQA+LRR KMNDM++FL SKQGS VEIK+ VF TV
Sbjct: 125 CGDRWKNLRSICRAKLFTARAIE--SQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSNFIFS DL+ FVGDGFNGIK  FKN++DLGL PNLADFYPLLR+FDLQGLRKKAA
Sbjct: 185 FNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAA 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY++QV+S WEILI+ERR IHS+   AA DFLD+LI+SGFTD+QI YLIVEVLSAGTDTT
Sbjct: 245 IYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAP 368
           TTTVEW MAELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAP
Sbjct: 305 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 364

Query: 369 FLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYD 428
           FLLPR APEACELMGYS+ KDT+IFVNVW IGRDPS+WEDP SF P+RF G D D KG+D
Sbjct: 365 FLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHD 424

Query: 429 FRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQ 488
           +R+LPFGGGRRICPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD NGRLGVTLQK+
Sbjct: 425 YRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKE 484

Query: 489 EPLKLIPKRR 497
           EPLKL+ K+R
Sbjct: 485 EPLKLLSKKR 489

BLAST of Lag0031968 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.3e-218
Identity = 371/483 (76.81%), Postives = 420/483 (86.96%), Query Frame = 0

Query: 17  LVVVPLAFIILRHI--NSKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSL 76
           L+++PL F IL+HI  +SKQ P PPGP+PWP+LGNLLQIGQ+AHISMT+F+ IYGPL+SL
Sbjct: 9   LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISL 68

Query: 77  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPECNGRWKTL 136
           KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LH++CSLVFSPEC  RWK L
Sbjct: 69  KLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNL 128

Query: 137 RSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTVFNTLSNFI 196
           RSICRA LFT RAIE  SQA+LRR KMN+M++FL SKQGS VEIK+FVF TVFNTLSNFI
Sbjct: 129 RSICRAKLFTARAIE--SQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 188

Query: 197 FSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQVNS 256
           FS DL+ FVGDGFNGI+  FKN++D  L PNLADFYPLLR FDLQGLRKKAAIY++Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 248

Query: 257 CWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTTTTTVEWTM 316
            WEILI+ERR IHS+      DFLD+LIQSGFTD+QI YLIVEVLSAGTDTTTTTVEW M
Sbjct: 249 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 308

Query: 317 AELLKNKDIMNKVREEMKEVINGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPRIAP 376
           AELLKN+DIMN+VREE K+ ING  +DES + +LHYLW CVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368

Query: 377 EACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLPFGG 436
           EACELMGYS+ K T+I VNVW IGRDPS+WEDP SF P+RF  CD D KG+D+R+LPFGG
Sbjct: 369 EACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFGG 428

Query: 437 GRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKLIPK 496
           GRR+CPG+PMAAVQ+PLILAT IHNFEW LPN ED S LD  GRLGVTLQK+EPLKL+PK
Sbjct: 429 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLPK 488

Query: 497 RRM 498
           RR+
Sbjct: 489 RRI 489

BLAST of Lag0031968 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 1.1e-209
Identity = 366/497 (73.64%), Postives = 421/497 (84.71%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHIN--SKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S S LLP+L+++   FIIL+HI   S + P PPGP PWPILGNLLQIGQ+AHIS+T+FA 
Sbjct: 5   SFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFAN 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
           IYGPL+SLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQMTPDR LH+  SLVFSPE
Sbjct: 65  IYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  +WK LRS+C+ NLFT +AIE  SQA LR  KM ++++FL+SKQGS VE+KDFVFT+V
Sbjct: 125 CGDQWKNLRSLCKVNLFTAKAIE--SQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSN IFS DLL FVGDGF GIK PFK MMDLGLTPNLADFYPLLR FDLQGL+ KA 
Sbjct: 185 FNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY+++V+S W ILI+ERR+IH Q   A+ DFLD+LIQSGF D QI  LI+E+LSAGTDTT
Sbjct: 245 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVI-NGSTVDESDIPQLHYLWNCVKETLRLHPPA 368
           TTTVEW M+E+LK+KDI+NKVREEMK+V      +DES+I +L YLW CVKETLRLHPPA
Sbjct: 305 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPA 364

Query: 369 PFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRF-VGC---DVD 428
           PFLLPR+AP  CE+MGYSI KD+++FVNVW IGRDPSVWED  +FNP RF VGC   +VD
Sbjct: 365 PFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVD 424

Query: 429 FKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGV 488
           FKGYD+RYLPFGGGRRICPG+PMA VQ+PLILAT IHNFEWSLPN EDLSKLD NGRLGV
Sbjct: 425 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGV 484

Query: 489 TLQKQEPLKLIPKRRMM 499
           TLQK E LKLIPKRR++
Sbjct: 485 TLQKYETLKLIPKRRVI 499

BLAST of Lag0031968 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.9e-209
Identity = 365/497 (73.44%), Postives = 421/497 (84.71%), Query Frame = 0

Query: 9   SSSFLLPALVVVPLAFIILRHIN--SKQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQ 68
           S S LLP+L+++   FIIL+HI   S + P PPGP PWPILGNLLQIGQ+AHIS+T+FA 
Sbjct: 5   SFSILLPSLLLLLPIFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFAN 64

Query: 69  IYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHCSLVFSPE 128
           IYGPL+SLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQMTPDR LH+  SLVFSPE
Sbjct: 65  IYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPE 124

Query: 129 CNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDSKQGSPVEIKDFVFTTV 188
           C  +WK LRS+C+ NLFT +AIE  SQA LR  KM ++++FL+SKQGS VE+KDFVFT+V
Sbjct: 125 CGDQWKNLRSLCKVNLFTAKAIE--SQAILREKKMKELVKFLESKQGSVVEVKDFVFTSV 184

Query: 189 FNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAA 248
           FNTLSN IFS DL+ FVGDGF GIK PFK MMDLGLTPNLADFYPLLR FDLQGL+ KA 
Sbjct: 185 FNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKAD 244

Query: 249 IYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILIQSGFTDDQIYYLIVEVLSAGTDTT 308
           IY+++V+S W ILI+ERR+IH Q   A+ DFLD+LIQSGF D QI  LI+E+LSAGTDTT
Sbjct: 245 IYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTT 304

Query: 309 TTTVEWTMAELLKNKDIMNKVREEMKEVI-NGSTVDESDIPQLHYLWNCVKETLRLHPPA 368
           TTTVEW M+E+LK+KDI+NKVREEMK+V      +DES+I +L YLW CVKETLRLHPPA
Sbjct: 305 TTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPA 364

Query: 369 PFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRF-VGC---DVD 428
           PFLLPR+AP  CE+MGYSI KD+++FVNVW IGRDPSVWED  +FNP RF VGC   +VD
Sbjct: 365 PFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVD 424

Query: 429 FKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGV 488
           FKGYD+RYLPFGGGRRICPG+PMA VQ+PLILAT IHNFEWSLPN EDLSKLD NGRLGV
Sbjct: 425 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGV 484

Query: 489 TLQKQEPLKLIPKRRMM 499
           TLQK E LKLIPKRR++
Sbjct: 485 TLQKYETLKLIPKRRVI 499

BLAST of Lag0031968 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-93
Identity = 185/483 (38.30%), Postives = 283/483 (58.59%), Query Frame = 0

Query: 33  KQAPNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAA 92
           K  P+PPGP   PI+GN+  +G++ H S    ++ YGP++SLK G+   VV +SP AA  
Sbjct: 34  KVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAARE 93

Query: 93  ILKTQDRLLSARYIFQMTPDRPL----HEHCSLVFSPECNGRWKTLRSICRANLFTGRAI 152
           +L+T D++LS+R     TP   +    H+  S+V+ P  + RW+ LR +    LF+ + I
Sbjct: 94  VLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRI 153

Query: 153 ESHSQATLRRAKMNDMLQFL--DSKQGSPVEIKDFVFTTVFNTLSNFIFSTDLLSFVGDG 212
           E  +  TLR  K+ +++ F+   S++   V+I    F T  N +SN +FS DL ++  + 
Sbjct: 154 E--ATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNK 213

Query: 213 FNGIKRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQVNSCWEILIQER--- 272
               +     +M+    P+ A+F+P L   DLQG RK      +++   +   I  +   
Sbjct: 214 SGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDAKLAE 273

Query: 273 ---RKIHSQSAPAAPDFLDILI------QSGFTDDQIYYLIVEVLSAGTDTTTTTVEWTM 332
              R  +S+      DF+D+L+      ++    + I +L++++  AGTDT ++TVEW M
Sbjct: 274 KSLRDTNSKDV-RERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAM 333

Query: 333 AELLKNKDIMNKVREEMKEVI-NGSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPRIA 392
           AELL+N + M K + E+  VI     V+ESDI  L YL   VKET RLHP AP L+PR A
Sbjct: 334 AELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLHPAAPLLVPRKA 393

Query: 393 PEACELMGYSISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLPFG 452
               E++G+ + KDT +FVNVWAIGRDP+VWE+   F P+RF+G D+D +G D+   PFG
Sbjct: 394 ESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFG 453

Query: 453 GGRRICPGIPMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKLIP 497
            GRRICPG+P+A   +PL+LA+ +++F+W LPNG     LD +   G+TL K  PL  +P
Sbjct: 454 AGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 508

BLAST of Lag0031968 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-92
Identity = 194/512 (37.89%), Postives = 293/512 (57.23%), Query Frame = 0

Query: 3   MDILSLSSSFLLPALVVVPLAFII-----LRHINSKQAPNPPGPRPWPILGNLLQIGQSA 62
           MDI+S  + FLL     +   F+I      R  + + A  PPGP   PI+GN+ Q+G++ 
Sbjct: 1   MDIISGQALFLL--FCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNP 60

Query: 63  HISMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHE 122
           H S    A+IYGP++SLK G    VV +SP AA  +L+T D++LS R           HE
Sbjct: 61  HSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HE 120

Query: 123 HCSLVFSPECNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLD--SKQGSP 182
             S+++ P  + RW+ LR +    +F+ +  E  +   LR  K+ +++ F++  S++   
Sbjct: 121 EVSVIWLPPSSARWRMLRKLSVTLMFSPQRTE--ATKALRMKKVQELVSFMNESSERKEA 180

Query: 183 VEIKDFVFTTVFNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRA 242
           V+I    +TTV N +SN +FS DL S+     N  +      M+    P+ A+++P +  
Sbjct: 181 VDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGF 240

Query: 243 FDLQGLRKKAAIYRDQV----NSCWEILIQERRKIHSQSAPAAPDFLDILI------QSG 302
            DLQG RK      +++        +  I E+   +     +  DFLD L+      ++ 
Sbjct: 241 LDLQGNRKAMRGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAE 300

Query: 303 FTDDQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNKDIMNKVREEMKEVI-NGSTVDESD 362
             ++ I +L++++ +AGTDT+++T+EW MAELL+N   M K + EM  V+   S V ESD
Sbjct: 301 LDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESD 360

Query: 363 IPQLHYLWNCVKETLRLHPPAPFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVW 422
           I  L YL   VKET RLHP AP L+PR A    E++G+ + KDT + VNVWAIGRDPSVW
Sbjct: 361 ISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVW 420

Query: 423 EDPLSFNPDRFVGCDVDFKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSL 482
           E+P  F P+RF+G D+D KG D+   PFGGGRRICPG+P+A   + L+LA+ +++F+W L
Sbjct: 421 ENPSQFEPERFMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKL 480

Query: 483 PNGEDLSKLDFNGRLGVTLQKQEPLKLIPKRR 497
           PNG     LD +   G+TL +   L  IP ++
Sbjct: 481 PNGVVSEDLDMDETFGITLHRTNTLYAIPVKK 507

BLAST of Lag0031968 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 334.0 bits (855), Expect = 2.1e-91
Identity = 190/510 (37.25%), Postives = 288/510 (56.47%), Query Frame = 0

Query: 3   MDILSLSSSFLLPALVVVPLAFIIL---RHINSKQAPNPPGPRPWPILGNLLQIGQSAHI 62
           MDI+S    FL+   ++  L F      R    + + +PPGP   PI+GN+  +G++ H 
Sbjct: 1   MDIISGQPLFLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHH 60

Query: 63  SMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHEHC 122
           S T  ++ YGP++SLKLG    VV +S  A   +LKT D++LS RYI + T     HE  
Sbjct: 61  SFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEF- 120

Query: 123 SLVFSPECNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFLDS--KQGSPVE 182
           S+ +    + R++ LR +    LF+ + I+  +   LR  K+ +++ FL    ++   V+
Sbjct: 121 SVGWIHPSSSRFRMLRKLSATQLFSPQCIQ--ATKALRMKKVQELVNFLSESCEREEAVD 180

Query: 183 IKDFVFTTVFNTLSNFIFSTDLLSFVGDGFNGIKRPFKNMMDLGLTPNLADFYPLLRAFD 242
           I    F T  N +SN +FS +L S+     +  +       +    P+LA+F+P +R  D
Sbjct: 181 ISHVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLD 240

Query: 243 LQG----LRKKAAIYRDQVNSCWEILIQERRKIHSQSAPAAPDFLDILI------QSGFT 302
           LQG    +R+ +          ++  I E+     +   ++ DFLD+LI      ++   
Sbjct: 241 LQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEIN 300

Query: 303 DDQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNKDIMNKVREEMKEVI-NGSTVDESDIP 362
            D+I +L++++  AGTDT ++TVEW MAELL N   M KV++E+  VI       ESDI 
Sbjct: 301 IDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDIS 360

Query: 363 QLHYLWNCVKETLRLHPPAPFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPSVWED 422
           +L YL   VKET RLHP APFLL R A    E++G+++ KD+ + VNVWAIGRDP VWE+
Sbjct: 361 KLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWEN 420

Query: 423 PLSFNPDRFVGCDVDFKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEWSLPN 482
           P  F P+RF+G ++D KG D+   PFG GRRICPG+P+A   + L+LA+ ++ FEW LPN
Sbjct: 421 PTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPN 480

Query: 483 GEDLSKLDFNGRLGVTLQKQEPLKLIPKRR 497
           G     LD     G+T+ K  PL  +P ++
Sbjct: 481 GVGSEDLDMEETFGLTVHKTNPLLAVPLKK 507

BLAST of Lag0031968 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 332.4 bits (851), Expect = 6.2e-91
Identity = 193/514 (37.55%), Postives = 295/514 (57.39%), Query Frame = 0

Query: 3   MDILSLSSSFLLPALVV-VPLAFIILR--HINSKQAPNPPGPRPWPILGNLLQIGQSAHI 62
           MDI+S  +  LL   ++   L F   R   I+      PPGP   PI+GN+  +G+  H 
Sbjct: 1   MDIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHR 60

Query: 63  SMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPL---- 122
           S    ++ YGP++SLKLG+   VV +SP AA  +L+T D++LSAR     +P   +    
Sbjct: 61  SFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSIN 120

Query: 123 HEHCSLVFSPECNGRWKTLRSICRANLFTGRAIESHSQATLRRAKMNDMLQFL--DSKQG 182
           H+  SLV+ P  + RW+ LR +    L + + IE  +   LR  K+ +++ F+   S + 
Sbjct: 121 HQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIE--ATKALRMNKVKELVSFISESSDRE 180

Query: 183 SPVEIKDFVFTTVFNTLSNFIFSTDLLSF-VGDGFNGIKRPFKNMMDLGLTPNLADFYPL 242
             V+I    F T  N +SN +FS DL S+      NG++    ++MD   TP+ A+++P 
Sbjct: 181 ESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPF 240

Query: 243 LRAFDLQGLRKKAAIYRDQVNSCW----EILIQERRKIHSQSAPAAPDFLDILI-----Q 302
           LR  DLQG  K   +  +++   +    +  I E+   ++    +  DF+D L+     +
Sbjct: 241 LRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDE 300

Query: 303 SGFTDDQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNKDIMNKVREEMKEVI-NGSTVDE 362
           S  +   I +L++++ +AGTDT+++T+EW M ELLKN   M K + E+  VI     V+E
Sbjct: 301 SELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEE 360

Query: 363 SDIPQLHYLWNCVKETLRLHPPAPFLLPRIAPEACELMGYSISKDTLIFVNVWAIGRDPS 422
           SDI +L YL   VKET RLH P P L+PR A    E++G+ + KDT + VNVWAIGRDPS
Sbjct: 361 SDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPS 420

Query: 423 VWEDPLSFNPDRFVGCDVDFKGYDFRYLPFGGGRRICPGIPMAAVQMPLILATFIHNFEW 482
           VW++P  F P+RF+G D+D +G D+   PFG GRRICPG+P+A   + L+LA+ +++F+W
Sbjct: 421 VWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDW 480

Query: 483 SLPNGEDLSKLDFNGRLGVTLQKQEPLKLIPKRR 497
            LP G     LD +   G+TL K  PL  +P ++
Sbjct: 481 KLPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507

BLAST of Lag0031968 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 325.9 bits (834), Expect = 5.8e-89
Identity = 188/474 (39.66%), Postives = 280/474 (59.07%), Query Frame = 0

Query: 35  APNPPGPRPWPILGNLLQIGQSAHISMTRFAQIYGPLVSLKLGAQRLVVASSPAAAAAIL 94
           A NPPGP    +L N+LQ  +  H S+   ++IYG ++S KLG    VV SSP  A  +L
Sbjct: 31  AKNPPGPSKLSLLRNILQTVEKPHRSLADLSRIYGSVMSFKLGCLTTVVISSPETAKEVL 90

Query: 95  KTQDRLLSARYIFQMTPDRPL-HEHCSLVFSPECNGRWKTLRSICRANLFTGRAIESHSQ 154
           KT D +LS  Y     P R   H   SL++ P    RW+ LR I R  LF+ + +E+ S 
Sbjct: 91  KTHDHVLS--YRVSSDPVRAAGHHELSLLWIPPL-ARWRFLRKITRNQLFSTQRLEATS- 150

Query: 155 ATLRRAKMNDMLQFLDS--KQGSPVEIKDFVFTTVFNTLSNFIFSTDLLSF-VGDGFNGI 214
             +R  K+ +++ F++   ++   V I    F T  N +SN +FST+L +F     F+  
Sbjct: 151 -AIRTRKVQELMNFVNKCCERREAVNISRASFITSLNIISNALFSTNLANFDDSKTFHDF 210

Query: 215 KRPFKNMMDLGLTPNLADFYPLLRAFDLQGLRKKAAIYRDQVNSCWEILIQERRKIHSQS 274
           +     MM++   PNLADF+P L   DLQG RK+A +   ++   ++  I  +R   S +
Sbjct: 211 QNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFIDTKR---SST 270

Query: 275 APAAPDFLDILI------QSGFTDDQIYYLIVEVLSAGTDTTTTTVEWTMAELLKNKDIM 334
           +    D LD L+      +S   D+ I +L++++  AG DT+++ VEW MAELL+N  ++
Sbjct: 271 SRNNNDMLDSLLDIAHKKESELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELLRNPKMI 330

Query: 335 NKVREEMKEVIN-GSTVDESDIPQLHYLWNCVKETLRLHPPAPFLLPRIA-PEACELMGY 394
            KV+EE+++VI    TV + DI +L YL   VKE+LRLHPPAPFL+PR +  +  ++  +
Sbjct: 331 VKVQEEIRQVIGLKGTVQDLDIVKLPYLQAVVKESLRLHPPAPFLVPRKSESDDVQIFEF 390

Query: 395 SISKDTLIFVNVWAIGRDPSVWEDPLSFNPDRFVGCDVDFKGYDFRYLPFGGGRRICPGI 454
            I K+T + VNVWAIGRDP+VW++P  F P+RF+G  +D KG  F  +PFG GRRICPG+
Sbjct: 391 LIPKNTQVLVNVWAIGRDPNVWKNPTQFEPERFLGRGIDVKGNHFELIPFGAGRRICPGM 450

Query: 455 PMAAVQMPLILATFIHNFEWSLPNGEDLSKLDFNGRLGVTLQKQEPLKLIPKRR 497
           P+A   M L+LA+ ++ F+W   NG     +D N   G TL K EPL ++P ++
Sbjct: 451 PLAFRIMHLVLASLLYGFDWEYQNGVVPENVDMNEAFGATLHKAEPLCIVPIKK 496