Lag0031170 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031170 vs. NCBI nr
Match: XP_022983261.1 (cytochrome P450 705A22-like [Cucurbita maxima])

HSP 1 Score: 247.7 bits (631), Expect = 6.2e-62
Identity = 125/144 (86.81%), Postives = 132/144 (91.67%), Query Frame = 0

Query: 1   MAEIQSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNS 60
           MAEIQS I LS LFFLS+ L+RSI +RTA KSTALR+PPSPPSLPLIGHLHLLSPALY+S
Sbjct: 1   MAEIQSLILLSTLFFLSVYLLRSILSRTAAKSTALRLPPSPPSLPLIGHLHLLSPALYSS 60

Query: 61  FRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGF 120
           FR LSDRYGP+LYLRLGASRCLVVSSAPIAAEIFKTHDV+FASRP LAFADKLYYGK GF
Sbjct: 61  FRLLSDRYGPILYLRLGASRCLVVSSAPIAAEIFKTHDVLFASRPTLAFADKLYYGKLGF 120

Query: 121 VTAPYGDYWRFMKKVCVTELLGVR 145
           V APYGDYWRFMKKVCVTELL  R
Sbjct: 121 VLAPYGDYWRFMKKVCVTELLSPR 144

BLAST of Lag0031170 vs. NCBI nr
Match: KAG6581589.1 (Beta-amyrin 24-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 246.9 bits (629), Expect = 1.1e-61
Identity = 124/144 (86.11%), Postives = 132/144 (91.67%), Query Frame = 0

Query: 1   MAEIQSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNS 60
           MAEI+S I L  LFFLS+ L+RSI +RTA KSTALR+PPSPPSLPLIGHLHLLSPALY+S
Sbjct: 1   MAEIESLILLFTLFFLSVYLLRSILSRTAAKSTALRLPPSPPSLPLIGHLHLLSPALYSS 60

Query: 61  FRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGF 120
           FRRLSDRYGP+LYLRLGASRCLVVSSAPIAAEIFKTHDV+FASRP LAFADKLYYGK GF
Sbjct: 61  FRRLSDRYGPILYLRLGASRCLVVSSAPIAAEIFKTHDVLFASRPTLAFADKLYYGKLGF 120

Query: 121 VTAPYGDYWRFMKKVCVTELLGVR 145
           V APYGDYWRFMKKVCVTELL  R
Sbjct: 121 VLAPYGDYWRFMKKVCVTELLSPR 144

BLAST of Lag0031170 vs. NCBI nr
Match: XP_022934891.1 (cytochrome P450 93A3-like [Cucurbita moschata])

HSP 1 Score: 246.1 bits (627), Expect = 1.8e-61
Identity = 124/144 (86.11%), Postives = 132/144 (91.67%), Query Frame = 0

Query: 1   MAEIQSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNS 60
           MAEI+S I LS LFFLS+ L+RSI +RTA  STALR+PPSPPSLPLIGHLHLLSPALY+S
Sbjct: 1   MAEIESLILLSTLFFLSVYLLRSILSRTAAISTALRLPPSPPSLPLIGHLHLLSPALYSS 60

Query: 61  FRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGF 120
           FRRLSDRYGP+LYLRLGASRCLVVSSAPIAAEIFKTHDV+FASRP LAFADKLYYGK GF
Sbjct: 61  FRRLSDRYGPILYLRLGASRCLVVSSAPIAAEIFKTHDVLFASRPTLAFADKLYYGKLGF 120

Query: 121 VTAPYGDYWRFMKKVCVTELLGVR 145
           V APYGDYWRFMKKVCVTELL  R
Sbjct: 121 VLAPYGDYWRFMKKVCVTELLSPR 144

BLAST of Lag0031170 vs. NCBI nr
Match: XP_038904342.1 (LOW QUALITY PROTEIN: cytochrome P450 705A22-like [Benincasa hispida])

HSP 1 Score: 224.2 bits (570), Expect = 7.4e-55
Identity = 118/144 (81.94%), Postives = 124/144 (86.11%), Query Frame = 0

Query: 1   MAEIQSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNS 60
           M EIQSF+ LSILF     LIR I T+TA KS ALR+PPSPPSLPLIGHLHLLSPALYNS
Sbjct: 1   MHEIQSFVLLSILFC----LIRYILTKTAAKS-ALRLPPSPPSLPLIGHLHLLSPALYNS 60

Query: 61  FRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGF 120
           FRRLSDRYGP+LYLRLGAS CLVV SAPIAAEIFKTHDV FASRP LAF DK+YYGKSGF
Sbjct: 61  FRRLSDRYGPILYLRLGASPCLVVLSAPIAAEIFKTHDVAFASRPPLAFPDKVYYGKSGF 120

Query: 121 VTAPYGDYWRFMKKVCVTELLGVR 145
           V+APY DYWRFMKK CVTELL  R
Sbjct: 121 VSAPYADYWRFMKKXCVTELLSPR 139

BLAST of Lag0031170 vs. NCBI nr
Match: XP_023521546.1 (cytochrome P450 705A22-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 212.2 bits (539), Expect = 2.9e-51
Identity = 102/111 (91.89%), Postives = 106/111 (95.50%), Query Frame = 0

Query: 34  ALRIPPSPPSLPLIGHLHLLSPALYNSFRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEI 93
           ALR+PPSPPSLPLIGHLHLLSPALY+SFRRLSDRYGP+LYLRLGASRCLVVSSAPIAAEI
Sbjct: 1   ALRLPPSPPSLPLIGHLHLLSPALYSSFRRLSDRYGPILYLRLGASRCLVVSSAPIAAEI 60

Query: 94  FKTHDVVFASRPILAFADKLYYGKSGFVTAPYGDYWRFMKKVCVTELLGVR 145
           FKTHDV+FASRP LAFADKLYYGK GFV APYGDYWRFMKKVCVTELL  R
Sbjct: 61  FKTHDVLFASRPTLAFADKLYYGKLGFVLAPYGDYWRFMKKVCVTELLSPR 111

BLAST of Lag0031170 vs. ExPASy Swiss-Prot
Match: Q0JFI2 (Cytochrome P450 93G1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G1 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.4e-29
Identity = 59/130 (45.38%), Postives = 90/130 (69.23%), Query Frame = 0

Query: 16  LSINLIRSIFTRTAVKSTA-LRIPPSPPSLPLIGHLHLLSPALYNSFRRLSDRYGPLLYL 75
           L++ L+  +    AV +    R+PPSP SLP+IGHLHLL P ++ +F  L+ R GPL+++
Sbjct: 21  LALALVVVVVVHVAVNAFGRRRLPPSPASLPVIGHLHLLRPPVHRTFHELAARLGPLMHV 80

Query: 76  RLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGFVTAPYGDYWRFMKK 135
           RLG++ C+V SSA +AAE+ ++H+   + RP+ A A +  Y  +GF  APY  +WRFMK+
Sbjct: 81  RLGSTHCVVASSAEVAAELIRSHEAKISERPLTAVARQFAYESAGFAFAPYSPHWRFMKR 140

Query: 136 VCVTELLGVR 145
           +C++ELLG R
Sbjct: 141 LCMSELLGPR 150

BLAST of Lag0031170 vs. ExPASy Swiss-Prot
Match: Q9LJY5 (Cytochrome P450 705A22 OS=Arabidopsis thaliana OX=3702 GN=CYP705A22 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.7e-28
Identity = 68/137 (49.64%), Postives = 91/137 (66.42%), Query Frame = 0

Query: 7   FIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLH-LLSPALYNSFRRLS 66
           FIF+ ++F L+       F +         +PPSPPSLP+IGH+H LLS   + S ++LS
Sbjct: 13  FIFI-LIFLLTFLCFFFFFKKPKDSRVNFDLPPSPPSLPIIGHVHLLLSTLTHKSLQKLS 72

Query: 67  DRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGFVTAPY 126
            RYGPLLYLR+     ++VSSA +A EIF+T DV  +SR + A  + L +G S FVTAPY
Sbjct: 73  SRYGPLLYLRIFNVPIILVSSASVAYEIFRTQDVNISSRGVTAVDESLVFGSSSFVTAPY 132

Query: 127 GDYWRFMKKVCVTELLG 143
           GDYW+FMKK+ V +LLG
Sbjct: 133 GDYWKFMKKLTVMKLLG 148

BLAST of Lag0031170 vs. ExPASy Swiss-Prot
Match: E9KBR8 (Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.8e-27
Identity = 59/138 (42.75%), Postives = 93/138 (67.39%), Query Frame = 0

Query: 5   QSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNSFRRL 64
           +S + + ++ F+S +L++ +F R       L+ PPSPP++P+IGHLHLL P +++SFR L
Sbjct: 4   ESLLLVFLIVFISASLLKLLFVRENKPKAHLKNPPSPPAIPIIGHLHLLKPLIHHSFRDL 63

Query: 65  SDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSGFVTAP 124
           S RYGPLL LR+G+ + +V S+  +A E  KT+++ ++SR +    + + Y  + F  AP
Sbjct: 64  SLRYGPLLSLRIGSVKFIVASTPSLAQEFLKTNELTYSSRKMNMAINMVTYHNATFAFAP 123

Query: 125 YGDYWRFMKKVCVTELLG 143
           Y  YW+FMKK+  TELLG
Sbjct: 124 YDTYWKFMKKLSTTELLG 141

BLAST of Lag0031170 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.5e-26
Identity = 66/134 (49.25%), Postives = 88/134 (65.67%), Query Frame = 0

Query: 12  ILFFLSINLI--RSIFTRTAVKSTALRIPPSPPSLPLIGHLHLLSPALYNSFRRLSDRYG 71
           +LF   I+ I   SI  R   K+  L +PPSP  LP+IGHLHLLSP  +  F +LS RYG
Sbjct: 5   VLFICLISTIVFASILWRKQNKNKTL-LPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYG 64

Query: 72  PLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFA-DKLYYGKSGFVTAPYGDY 131
           P+++L LG+  C+V S+A  A E  KTH+  F++RP    A + L YG   F+ APYG Y
Sbjct: 65  PIIHLFLGSVPCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPY 124

Query: 132 WRFMKKVCVTELLG 143
           W+FMKK+C++ELLG
Sbjct: 125 WKFMKKLCMSELLG 137

BLAST of Lag0031170 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.4e-26
Identity = 62/142 (43.66%), Postives = 92/142 (64.79%), Query Frame = 0

Query: 1   MAEIQSFIFLSILFFLSINLIRSIFTRTAVKSTALRIPPSPP-SLPLIGHLHLLSPALYN 60
           M +I+ ++ L  L+F+S  LIRSIF     K   LR+PP PP S+PL+GH   L   L+ 
Sbjct: 1   MLDIKGYLVLFFLWFISTILIRSIFK----KPQRLRLPPGPPISIPLLGHAPYLRSLLHQ 60

Query: 61  SFRRLSDRYGPLLYLRLGASRCLVVSSAPIAAEIFKTHDVVFASRPILAFADKLYYGKSG 120
           +  +LS RYGPL+++ +G+   +V SSA  A +I KT +  F +RP++  ++ L YG + 
Sbjct: 61  ALYKLSLRYGPLIHVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAAD 120

Query: 121 FVTAPYGDYWRFMKKVCVTELL 142
           +   PYG YWRF+KK+C+TELL
Sbjct: 121 YFFIPYGTYWRFLKKLCMTELL 138