Lag0031061 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0031061 vs. NCBI nr
Match: XP_023524602.1 (LOW QUALITY PROTEIN: probable pectinesterase 55 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.8 bits (1096), Expect = 1.4e-115
Identity = 196/262 (74.81%), Postives = 221/262 (84.35%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I + KSCIFL+GAGR  TEI+WNDH TTATSATFTS A+NL+V+GITF+NTYN PG
Sbjct: 77  EKVHISEEKSCIFLEGAGRSSTEIRWNDHATTATSATFTSIAENLVVKGITFKNTYNVPG 136

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  +  IAPA+AALI+ DKG F+KCGF+GLQDTLWDG GRH Y  CYIEGVIDVISGA 
Sbjct: 137 SVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAG 196

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIYE C INIPV++Y  ++ HGYITAQGKED S+TNGFVFIGCSV GSG VYLGRAYRP
Sbjct: 197 QSIYESCEINIPVDRYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRP 256

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVI++ SFL  CI+PAGWD W QVGHE  LTY ETRCIGPGAD SKRVPWLKKLS  +
Sbjct: 257 FSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYLETRCIGPGADTSKRVPWLKKLSIDE 316

Query: 254 VKSFIDISFIDQEGWTSKLPLY 276
           ++ F  ISF+DQEGWTSKLPL+
Sbjct: 317 IRRFTSISFVDQEGWTSKLPLF 338

BLAST of Lag0031061 vs. NCBI nr
Match: XP_022157021.1 (uncharacterized protein LOC111023848 [Momordica charantia])

HSP 1 Score: 421.0 bits (1081), Expect = 7.9e-114
Identity = 191/262 (72.90%), Postives = 220/262 (83.97%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV+I  GK+CIFLDGA  R TEIQWNDH  TATSATFTSFA+NLLV+GITF+NTYN PG
Sbjct: 391 EKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPG 450

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  R  I PALA LI+ DK SFY+C FIGLQDTLWDG GRHH++ CYIEGVID+ISG+ 
Sbjct: 451 SVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSG 510

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIYEKC IN+P+++Y  IL +GYITAQGKE+P+E +GFVF  CSV G+GRVYLGRAYRP
Sbjct: 511 QSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRP 570

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVIFH SFL   IDPAGWD W QVGHE DLTY+E  CIGPGAD S+RVPWLK+LS A+
Sbjct: 571 FSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSAAE 630

Query: 254 VKSFIDISFIDQEGWTSKLPLY 276
           +K F  ISFIDQ+GWTS+LP++
Sbjct: 631 LKYFSHISFIDQDGWTSRLPIF 652

BLAST of Lag0031061 vs. NCBI nr
Match: KAG6606930.1 (putative pectinesterase 55, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 418.7 bits (1075), Expect = 3.9e-113
Identity = 194/262 (74.05%), Postives = 218/262 (83.21%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I + KSCIFLDGAGR  TEI+WNDH TTATSATFTS A+NL+V+GITF+NTYNAPG
Sbjct: 77  EKVHISEEKSCIFLDGAGRSSTEIRWNDHATTATSATFTSIAENLVVKGITFKNTYNAPG 136

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  +  IAPA+AALI+ DKG F+KCGF+GLQDTLWDG GRH Y  CYIEGVIDVISGA 
Sbjct: 137 SVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVIDVISGAG 196

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIYE C IN+PV+ Y  ++ HGYITAQGKED S+TNGFVFIGCSV GSG VYLGRAYRP
Sbjct: 197 QSIYENCEINVPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYRP 256

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVI++ SFL  CIDPAGWD W QVGHE  LT +ET C+G GAD SKRV WLKKLS  +
Sbjct: 257 FSTVIYYSSFLSACIDPAGWDPWEQVGHEASLTCAETHCVGLGADTSKRVSWLKKLSIDE 316

Query: 254 VKSFIDISFIDQEGWTSKLPLY 276
           ++ F  ISFIDQEGWTSKLPL+
Sbjct: 317 IRHFTSISFIDQEGWTSKLPLF 338

BLAST of Lag0031061 vs. NCBI nr
Match: KAG6606929.1 (putative pectinesterase 29, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.5 bits (1020), Expect = 9.4e-107
Identity = 186/262 (70.99%), Postives = 206/262 (78.63%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I   K CIFLDG  R LTEIQW+DH TTA+SATFT  A NL+V+GITF+NTYN PG
Sbjct: 14  EKVRIPPEKPCIFLDGVDRNLTEIQWSDHETTASSATFTFSAVNLVVRGITFKNTYNMPG 73

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SVT+RN I PALAA +  DK  F++CGF GLQDTLWDG GRH Y  CYIEGVIDVISG  
Sbjct: 74  SVTSRNDIKPALAAFVDGDKAVFHQCGFYGLQDTLWDGPGRHRYTECYIEGVIDVISGTG 133

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QS+YEKCVINIP+N Y  IL HGYITAQGKE  S TNGFVFI CSV GSG VYLGRAYR 
Sbjct: 134 QSMYEKCVINIPINVYAPILGHGYITAQGKEASSHTNGFVFIECSVIGSGTVYLGRAYRR 193

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVI+H SFL  CI+P GW  W QVGHEMD TY E+ C GPGADISKRVPW+K L+   
Sbjct: 194 FSTVIYHKSFLSSCINPVGWHPWTQVGHEMDFTYVESSCTGPGADISKRVPWIKNLNKYD 253

Query: 254 VKSFIDISFIDQEGWTSKLPLY 276
           ++ FIDISFIDQ+GWT KLP +
Sbjct: 254 LRRFIDISFIDQDGWTRKLPQF 275

BLAST of Lag0031061 vs. NCBI nr
Match: XP_011652807.1 (probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 6.1e-106
Identity = 184/262 (70.23%), Postives = 213/262 (81.30%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I   KSCIFLDG+G ++TEI WNDH TTA S TFT+ A NL+VQGITFRNTYNA G
Sbjct: 33  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 92

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  R  I PALAALIQ DK  F+KCGFIGLQDTLWDG GRH +  CYIEGVIDVISG  
Sbjct: 93  SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 152

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIY++CVINIPVN Y  +L+ G+ITAQGKE+P+E++GFVF+ C V GSG V+LGRAYRP
Sbjct: 153 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 212

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVIFHL FLP CI+PAGW++W Q GHE DLTYSETRCIGPGAD S RVPW+ +L    
Sbjct: 213 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 272

Query: 254 VKSFIDISFIDQEG-WTSKLPL 275
           ++SF DIS+ID +G WTS++PL
Sbjct: 273 IRSFTDISYIDPQGYWTSRIPL 294

BLAST of Lag0031061 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 5.6e-70
Identity = 126/262 (48.09%), Postives = 172/262 (65.65%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EK+ I   K  I L GAG+RLT ++W+DH + A S TF++ ADN +V+ ITF N+YN P 
Sbjct: 73  EKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPS 132

Query: 74  -SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
                +N   PA+AALI  DK +FY  GF G+QDTLWD  GRH++  C I+G +D I G 
Sbjct: 133 KGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGT 192

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QSIY+ CVI +   +    L  GYITAQG+ +P + NGF+FI C V+G+G  +LGR +R
Sbjct: 193 GQSIYQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWR 252

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
            +S VIF+ S L   + P GWDAW  VGHE  L ++E  C G GA+I +RV W+KKLS +
Sbjct: 253 GYSRVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSES 312

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
            +++  D+SFI++ GW   LP+
Sbjct: 313 AIQNLADLSFINRGGWVEDLPI 333

BLAST of Lag0031061 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 7.3e-70
Identity = 124/262 (47.33%), Postives = 171/262 (65.27%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EK+ I + K  I + GAG+R T ++W+DH + A S TF + ADN +V+ ITF N+YN P 
Sbjct: 68  EKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPS 127

Query: 74  S-VTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
           +    +N   PA+AA I  DK +FY  GF G+QDTLWD  GRH++  C I+G +D I G+
Sbjct: 128 NGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGS 187

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QSIY+ CVI +   + G  +  GYITAQG+ + ++ NGFVFI C V G G+ YLGRA+R
Sbjct: 188 GQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWR 247

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
           P+S VIF+ S L   +DP GW  W   G+E  LTY+E  C G G++ S+R  W+KKLS +
Sbjct: 248 PYSRVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSAS 307

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
            V+   D+SFI++ GW   LP+
Sbjct: 308 AVQHLADLSFINRGGWVEDLPI 328

BLAST of Lag0031061 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 8.4e-66
Identity = 124/262 (47.33%), Postives = 168/262 (64.12%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYN-AP 73
           EKV I K K  I+L G G   T I+++DH  T  SATFT+FAD++++ GITF+NTYN  P
Sbjct: 74  EKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP 133

Query: 74  GSVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
            +   + +I PA+AA +  D+       F+GLQDTL+DG+GRH+Y  C I G ID I G 
Sbjct: 134 NN---KREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGY 193

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QS++++C +N+ +  Y     +G ITA  +  PS+  GFVF  C+V G G+  LGRA+ 
Sbjct: 194 GQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWG 253

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
             + VIF  S L   + P GWDAW   G+E DLT+ E  C G GAD S+RVPWLKKLS +
Sbjct: 254 SNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLS 313

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
           +V  F  +SFIDQ+GW S+ P+
Sbjct: 314 EVDGFASVSFIDQDGWISRFPI 332

BLAST of Lag0031061 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.2e-65
Identity = 123/261 (47.13%), Postives = 161/261 (61.69%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I +GK  I++ G G   T I + DH  T TSATFTS+  N+++ GITF+N YN   
Sbjct: 80  EKVTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIAS 139

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           S    +   PA+AA++  DK +     F G QDTL+D  GRH+Y  C I G ID I G A
Sbjct: 140 S---SSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGA 199

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSI+E C + + V  Y     +G ITAQG++ P++  GFVF  C+V GSG+  LGRA++ 
Sbjct: 200 QSIFEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKS 259

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           +S VIF+ S     I P GWDAW   G E  +T+ E  C G GAD SKRVPWL K S   
Sbjct: 260 YSRVIFYRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKD 319

Query: 254 VKSFIDISFIDQEGWTSKLPL 275
           V  F +++FID+EGW S+LP+
Sbjct: 320 VLQFTNLTFIDEEGWLSRLPI 337

BLAST of Lag0031061 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 248.4 bits (633), Expect = 9.2e-65
Identity = 119/252 (47.22%), Postives = 157/252 (62.30%), Query Frame = 0

Query: 29  GAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPGSVTARN----QIAPA 88
           G G+R+T I +N H  T  S+TFTS+  +++V+ ++  NTYN   S+T  N     I PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 89  LAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAAQSIYEKCVINI 148
           +A  +  DK +FY C F+GLQDT+WD  GRHH+  CYIEG ID I G+ QS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 149 PVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRPFSTVIFHLSFL 208
                 + +  GYITAQG+   S+ +GFVF+  SV GS  VYLGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 209 PPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAKVKSFIDIS-FI 268
              + P GW +W   G+EM  TY+E  C G G+D+S+RVPW+ KL     K    IS FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 269 DQEGWTSKLPLY 276
           DQ+ W S +P +
Sbjct: 282 DQDQWISNIPRF 293

BLAST of Lag0031061 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.8e-114
Identity = 191/262 (72.90%), Postives = 220/262 (83.97%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV+I  GK+CIFLDGA  R TEIQWNDH  TATSATFTSFA+NLLV+GITF+NTYN PG
Sbjct: 391 EKVVIPAGKTCIFLDGADNRFTEIQWNDHQATATSATFTSFAENLLVKGITFKNTYNTPG 450

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  R  I PALA LI+ DK SFY+C FIGLQDTLWDG GRHH++ CYIEGVID+ISG+ 
Sbjct: 451 SVRRREDIEPALAVLIEGDKASFYECRFIGLQDTLWDGTGRHHFVNCYIEGVIDIISGSG 510

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIYEKC IN+P+++Y  IL +GYITAQGKE+P+E +GFVF  CSV G+GRVYLGRAYRP
Sbjct: 511 QSIYEKCKINVPIDQYSPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVYLGRAYRP 570

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVIFH SFL   IDPAGWD W QVGHE DLTY+E  CIGPGAD S+RVPWLK+LS A+
Sbjct: 571 FSTVIFHNSFLAGFIDPAGWDPWEQVGHERDLTYAEANCIGPGADTSRRVPWLKRLSAAE 630

Query: 254 VKSFIDISFIDQEGWTSKLPLY 276
           +K F  ISFIDQ+GWTS+LP++
Sbjct: 631 LKYFSHISFIDQDGWTSRLPIF 652

BLAST of Lag0031061 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.9e-106
Identity = 184/262 (70.23%), Postives = 213/262 (81.30%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I   KSCIFLDG+G ++TEI WNDH TTA S TFT+ A NL+VQGITFRNTYNA G
Sbjct: 85  EKVTIPSEKSCIFLDGSGLQVTEIHWNDHETTAASPTFTASAQNLVVQGITFRNTYNARG 144

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           SV  R  I PALAALIQ DK  F+KCGFIGLQDTLWDG GRH +  CYIEGVIDVISG  
Sbjct: 145 SVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFG 204

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSIY++CVINIPVN Y  +L+ G+ITAQGKE+P+E++GFVF+ C V GSG V+LGRAYRP
Sbjct: 205 QSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVFLGRAYRP 264

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           FSTVIFHL FLP CI+PAGW++W Q GHE DLTYSETRCIGPGAD S RVPW+ +L    
Sbjct: 265 FSTVIFHLCFLPSCINPAGWNSWLQAGHESDLTYSETRCIGPGADTSSRVPWVNRLDAFH 324

Query: 254 VKSFIDISFIDQEG-WTSKLPL 275
           ++SF DIS+ID +G WTS++PL
Sbjct: 325 IRSFTDISYIDPQGYWTSRIPL 346

BLAST of Lag0031061 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.8e-103
Identity = 180/254 (70.87%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 22  KSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPGSVTARNQI 81
           KSCIFLDG+G R+TEIQWNDH TTATS TFTS+A NL+       NTYNA GSVT R  I
Sbjct: 6   KSCIFLDGSGFRVTEIQWNDHATTATSPTFTSYAQNLV-------NTYNALGSVTRREDI 65

Query: 82  APALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAAQSIYEKCV 141
            PA+AALIQ DK  F+KCGFIGLQDTLWDG GRH +  CYIEGVIDVISG  QSIY++CV
Sbjct: 66  KPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGQSIYKECV 125

Query: 142 INIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRPFSTVIFHL 201
           INIP+N Y  +L+ G+ITAQGKE+PSE++GFVFI C V GSG+V+LGRAYRPFSTVIFHL
Sbjct: 126 INIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAYRPFSTVIFHL 185

Query: 202 SFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAKVKSFIDIS 261
            FLP CIDPAGW++W QVGHE +LTYSETRCIGPGAD S RVPWLK+L   +++ F DIS
Sbjct: 186 CFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDVYQIRRFTDIS 245

Query: 262 FIDQEG-WTSKLPL 275
           +ID +G WTS+LP+
Sbjct: 246 YIDPQGYWTSRLPV 252

BLAST of Lag0031061 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 1.1e-100
Identity = 178/264 (67.42%), Postives = 207/264 (78.41%), Query Frame = 0

Query: 28  DGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPGSVTARNQIAPALAA 87
           DG+G R+TEIQWNDH TTATS TFTS+A NL+VQGITFRNTYNA GSVT R  I PA+AA
Sbjct: 85  DGSGFRVTEIQWNDHATTATSPTFTSYAQNLVVQGITFRNTYNALGSVTRREDIKPAIAA 144

Query: 88  LIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAAQSI----------- 147
           LIQ DK  F+KCGFIGLQDTLWDG GRH +  CYIEGVIDVISG    I           
Sbjct: 145 LIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVIDVISGFGHQIEVLIFFLFVCL 204

Query: 148 -----YEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAY 207
                 ++CVINIP+N Y  +L+ G+ITAQGKE+PSE++GFVFI C V GSG+V+LGRAY
Sbjct: 205 FVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVFLGRAY 264

Query: 208 RPFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSG 267
           RPFSTVIFHL FLP CIDPAGW++W QVGHE +LTYSETRCIGPGAD S RVPWLK+L  
Sbjct: 265 RPFSTVIFHLCFLPSCIDPAGWNSWQQVGHESNLTYSETRCIGPGADTSSRVPWLKRLDV 324

Query: 268 AKVKSFIDISFIDQEG-WTSKLPL 275
            +++ F DIS+ID +G WTS+LP+
Sbjct: 325 YQIRRFTDISYIDPQGYWTSRLPV 348

BLAST of Lag0031061 vs. ExPASy TrEMBL
Match: A0A6J1GAV4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452500 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 9.9e-86
Identity = 147/203 (72.41%), Postives = 166/203 (81.77%), Query Frame = 0

Query: 73  GSVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 132
           GSV  +  IAPA+AALI+ DKG F+KCGF+GLQDTLWDG GRH Y  CYIEGV DVISGA
Sbjct: 44  GSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVTDVISGA 103

Query: 133 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 192
            QSIYE C INIPV+ Y  ++ HGYITAQGKED S+TNGFVFIGCSV GSG VYLGRAYR
Sbjct: 104 GQSIYENCEINIPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAYR 163

Query: 193 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 252
           PFSTVI++ SFL  CI+PAGWD W QVGHE  LTY+ETRC+GPGAD SKRV WL+ LS  
Sbjct: 164 PFSTVIYYSSFLSACINPAGWDPWEQVGHEASLTYAETRCVGPGADTSKRVSWLQNLSID 223

Query: 253 KVKSFIDISFIDQEGWTSKLPLY 276
           +++ F  ISFIDQEGWT KLPL+
Sbjct: 224 EIRHFTSISFIDQEGWTLKLPLF 246

BLAST of Lag0031061 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.0e-71
Identity = 126/262 (48.09%), Postives = 172/262 (65.65%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EK+ I   K  I L GAG+RLT ++W+DH + A S TF++ ADN +V+ ITF N+YN P 
Sbjct: 73  EKIKIPYEKPFIVLVGAGKRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPS 132

Query: 74  -SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
                +N   PA+AALI  DK +FY  GF G+QDTLWD  GRH++  C I+G +D I G 
Sbjct: 133 KGKMNKNPRTPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGT 192

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QSIY+ CVI +   +    L  GYITAQG+ +P + NGF+FI C V+G+G  +LGR +R
Sbjct: 193 GQSIYQSCVIQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWR 252

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
            +S VIF+ S L   + P GWDAW  VGHE  L ++E  C G GA+I +RV W+KKLS +
Sbjct: 253 GYSRVIFYNSNLTDVVVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSES 312

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
            +++  D+SFI++ GW   LP+
Sbjct: 313 AIQNLADLSFINRGGWVEDLPI 333

BLAST of Lag0031061 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 5.2e-71
Identity = 124/262 (47.33%), Postives = 171/262 (65.27%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EK+ I + K  I + GAG+R T ++W+DH + A S TF + ADN +V+ ITF N+YN P 
Sbjct: 68  EKITIPQKKPFIVIVGAGKRSTRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPS 127

Query: 74  S-VTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
           +    +N   PA+AA I  DK +FY  GF G+QDTLWD  GRH++  C I+G +D I G+
Sbjct: 128 NGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGS 187

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QSIY+ CVI +   + G  +  GYITAQG+ + ++ NGFVFI C V G G+ YLGRA+R
Sbjct: 188 GQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWR 247

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
           P+S VIF+ S L   +DP GW  W   G+E  LTY+E  C G G++ S+R  W+KKLS +
Sbjct: 248 PYSRVIFYNSNLTDVVDPLGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSAS 307

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
            V+   D+SFI++ GW   LP+
Sbjct: 308 AVQHLADLSFINRGGWVEDLPI 328

BLAST of Lag0031061 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 251.9 bits (642), Expect = 5.9e-67
Identity = 124/262 (47.33%), Postives = 168/262 (64.12%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYN-AP 73
           EKV I K K  I+L G G   T I+++DH  T  SATFT+FAD++++ GITF+NTYN  P
Sbjct: 74  EKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVP 133

Query: 74  GSVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGA 133
            +   + +I PA+AA +  D+       F+GLQDTL+DG+GRH+Y  C I G ID I G 
Sbjct: 134 NN---KREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGY 193

Query: 134 AQSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYR 193
            QS++++C +N+ +  Y     +G ITA  +  PS+  GFVF  C+V G G+  LGRA+ 
Sbjct: 194 GQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWG 253

Query: 194 PFSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGA 253
             + VIF  S L   + P GWDAW   G+E DLT+ E  C G GAD S+RVPWLKKLS +
Sbjct: 254 SNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLS 313

Query: 254 KVKSFIDISFIDQEGWTSKLPL 275
           +V  F  +SFIDQ+GW S+ P+
Sbjct: 314 EVDGFASVSFIDQDGWISRFPI 332

BLAST of Lag0031061 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 6.6e-66
Identity = 123/261 (47.13%), Postives = 161/261 (61.69%), Query Frame = 0

Query: 14  EKVIILKGKSCIFLDGAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPG 73
           EKV I +GK  I++ G G   T I + DH  T TSATFTS+  N+++ GITF+N YN   
Sbjct: 80  EKVTIPRGKGYIYMQGGGIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIAS 139

Query: 74  SVTARNQIAPALAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAA 133
           S    +   PA+AA++  DK +     F G QDTL+D  GRH+Y  C I G ID I G A
Sbjct: 140 S---SSPTKPAVAAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGA 199

Query: 134 QSIYEKCVINIPVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRP 193
           QSI+E C + + V  Y     +G ITAQG++ P++  GFVF  C+V GSG+  LGRA++ 
Sbjct: 200 QSIFEGCTLKLRVGIYPPNEVYGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKS 259

Query: 194 FSTVIFHLSFLPPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAK 253
           +S VIF+ S     I P GWDAW   G E  +T+ E  C G GAD SKRVPWL K S   
Sbjct: 260 YSRVIFYRSMFSDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKD 319

Query: 254 VKSFIDISFIDQEGWTSKLPL 275
           V  F +++FID+EGW S+LP+
Sbjct: 320 VLQFTNLTFIDEEGWLSRLPI 337

BLAST of Lag0031061 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 6.6e-66
Identity = 119/252 (47.22%), Postives = 157/252 (62.30%), Query Frame = 0

Query: 29  GAGRRLTEIQWNDHTTTATSATFTSFADNLLVQGITFRNTYNAPGSVTARN----QIAPA 88
           G G+R+T I +N H  T  S+TFTS+  +++V+ ++  NTYN   S+T  N     I PA
Sbjct: 42  GEGQRVTTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYNRLTSLTKANGMSWDIKPA 101

Query: 89  LAALIQADKGSFYKCGFIGLQDTLWDGRGRHHYILCYIEGVIDVISGAAQSIYEKCVINI 148
           +A  +  DK +FY C F+GLQDT+WD  GRHH+  CYIEG ID I G+ QS+YE C IN 
Sbjct: 102 VAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINA 161

Query: 149 PVNKYGAILDHGYITAQGKEDPSETNGFVFIGCSVFGSGRVYLGRAYRPFSTVIFHLSFL 208
                 + +  GYITAQG+   S+ +GFVF+  SV GS  VYLGRAY PFS VIF  + L
Sbjct: 162 TAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDL 221

Query: 209 PPCIDPAGWDAWGQVGHEMDLTYSETRCIGPGADISKRVPWLKKLSGAKVKSFIDIS-FI 268
              + P GW +W   G+EM  TY+E  C G G+D+S+RVPW+ KL     K    IS FI
Sbjct: 222 SSVVHPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFI 281

Query: 269 DQEGWTSKLPLY 276
           DQ+ W S +P +
Sbjct: 282 DQDQWISNIPRF 293