Lag0031033 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031033
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionkinesin-like protein KIN-14I
Locationchr11: 3992290 .. 3995670 (-)
RNA-Seq ExpressionLag0031033
SyntenyLag0031033
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGAACGCTGTCGTTTTCAGTGGCATCTGTGGTGGAGGATGTTCTTCAACAGCATGGGAATCGTCTTGGTAATGGAGGAGGAGGCCTTGATTTGGAATCTAGAAGAGCTGAGGAAGCTGGTAACTCTGCTTTTTCTAATCTTCTTTTTTTCCCAAATCTTTGGAGTCTTGTGAATCTGATGAATGAGTCTGGTAATCGATTCACTGATGTGGGTTTTTCAAAGAGAGAGAGAGCGTATGCTTGTATTTTGGATATACATTCCTAACAACGAGAAAAATTTGAGTATCTTAATTACTCTCTTTAGAAATGATAGGGATTTGTGATCCTCTGTTTTTTCCATCTACTCGATCGATTCTGTACTGAGATTTTTTCTTTTAATAGTTCTGTATGAGTTCACTCAAACTCAGGATATACAATTGTTTTTGAATTTGTGTTGGAATTGTTATTTGTGACAATGTGGGCAGTGGTTATTTGAAGTGTTAAACCTGATCTCTTGACTTTTTAAAACTTTTGGGGTTTATTGGGTTTTCTAGCTATTTTTTCTGATACTTATTGAGATTGGTTCTTTATACTAGCATCTAGAAGAAACGAAGCTGCTGGATGGCTGAGAAAGATGATCGGGGTTGTTGCCGCTAAAGATTTGCCAGCTGAACCTTCAGAAGAAGAATTCAGGCTTGGATTGAGAAGTGGGATTATTCTCTGCAATGTCCTGAATAAGGTCCAACCAGGAGCAGTGCCCAAGGTGAGCCAAATTCACATTTGGGTTTGTTGTTTTTTGGTCAGTATATGTATTAAAATCTTCTCTTTGCTTATCTCTCACAAAATTTATTCTCAGGTGGTTGAAAGTCCTTGTGATTCTGCCCTCATCCCTGATGGAGCTGCACTCTCAGCATTTCAGTATTTTGAGAACGTGAGGAATTTTCTTGTAGCTATTCAGGAGATGGGAGTTCCTACCTTTGAGGCATCTGATCTAGAGCAAGTACGTATGAAGAGAGAAAAAGAAAAAGATGACTGAGTTCTAAGAAAAGGGTCCCTTGATTGACAAGTTTCTATATGATATTCTTTTTCAGGGAGGGACATCTGCCAGAGTAGTCAATACTGTTCTAGCTCTAAAATCTTATGGCGAGTGGAAACAGGGTGGAGGCTATGGAGTTTGGAAATTCGGTGGAAACGTGAAACCTACAACTATGTCCGCTACTAAGTCCTTTGTGAGGAAAAATTCAGAGCCATTCACAAATTCCTTGTCAAGAACCTCATCTCTGAATGACAAAGCCTTCAATTCTTCCAACGTTGAATGGAACAAAACGGTTAGTAATGTGGATTTTTTCAACCTTGCGTTATTTGCTTTGTTTGTCTCAAGTTTATTTGTTTTAACCTTTTTTTAACTCTACTTTCTTTCTTGTCTATTATTCATGGTAGCAGACTAGTTCTCGCTCCTCGCTCATTCGTGCACTTTTAACGGATAAACGGCCTGAAGAAATCCCAACGGTAATGACTGAATATATGTTGTTGCTCCTCGAGTACTTTTTGTTCATGTAGCTTCCTGATAGAATCTGTTGGTGCTTGGTGGTAGCCTTAGATCAAGATAAATGGATATTGTTCATAAAATTGAGGTTTTGTCCCCCAGACTGTAAAGAGTTATTGAGAAGAAAGAGCTAAAATAGTTTGTATTTTGACACAATTCATCAATCTAATCTTTATTCTGAGAATAGTATTTGCTTTATTCTTTTTCAGATACTAGGCTAAGAGCATGTGTAGTTTAGTGTACTATTTTGTATTACATGAAGGTGTCCACAACTTTTTCTAAACTATGTTGCTCTATCTTGTAATAGTTGGTAGAATCATTGCTAAGCAAGCTTGTGGATGAAGTTGAGAATCGCTTTTCCAGCCTTGAACTGGTATGTTAGATTCAACAATCTTGTTTACTTCATTTTGTGCCTTCGCATTTTGTTTATTAGCCTACTATTTTACACTACAGACAAAAGTAAATTCAAAAGATGTTGTTGCTGCTACATCACAAGGAAACAAGTTGCACTTCAAGTCAGCTTTTGGTGCTAAAAGGGTGTGTTGTCGATCTTTTATACTCACACAGCCTACAGTTGCAGTATTGCCAAATTTTATGAATTTACTCTTTTCACTCTTTGTATAGGCAGAGGAACTTAACTCCAAAGCCACGGAAAAGAATGAAATAATACATGAAAGCAGTATATTTGAGGAGCAATCAAAGAGCCTGCTCCTGAAGCAGCAAACGATTTTCGATCAGCAACAAAAGGATGTTCAGGTATGCAACTGTAACATATTCTTACTAATTGTGTTTTTATTTGTTGTTCACCATGTTCGTACTAATTTTGAATTATTATCGTAGGAACTAAAACATAAGCTGCACGCTGCGAAAGCTGGAATGCAGTTTATGCAAGTAAAATTTAGTGAAGAGTTCCAGAACCTCGGTAGGATTATTGGATATATTTATCAGTGGCAAATTTCGTGCGGAGTAGATAACTTTTTGTCTCATTTCAAATTTTTGGCATCTTTTTTAATTTTTTTATGTAGGTATGCATGTACATAGTCTTGCTCATGCTGCATCTGGATATCATAAAGTTCTCGAGGAAAACCGTAAGCTATACAACCAAGTGCAGGATCTCAAGGGTAATTTAGATCACTGGTCTTATAAGCTTGAATTTTCTGGGCATTTCATCTTGAAGCTTATAAATGTTTTGGTCTTATAATGACCAGGTAGCATAAGAGTTTACTGCCGAGTTAGACCCTTTTTGTCTGGTCAATCAAATTATTTGAGTGTCGTGGATCACATAGAAGATGGAAATATTACTGTTAACGCCCCCTCAAAGCATGGGAAGGGACAGAGATCATTCAGCTTCAATAAAGTATTTGGTCCATCTGCTACACAAGGTTGATGAAATTCAAATTTGATGAAGGGGTATGGTTCTTTTAACGCTTAATTTTGACCATTTGTTCTCATTCTATTGCAGTGGAGGTCTTTGCTGATATGCAGCCACTCATTCGGTCTGTTCTTGATGGATACAATGTTTGCATTTTGCATATGGACAAACAGGATCAGGGAAAACTTTTACTATGGTATGTATCAAGCATTTGAAGTTTACCTCTAAAAAGCAAGAGTATGTGAAATTCAGATCTGTGCTCATCTCTTTCATTTTCTCATGTCTAGACTGGGCCAAAAGAGCTAACAGAGAAGAGTCAAGGAGTAAATTATAGAGCATTGGGAGATTTGTTCCTTATAGCAGATCAAAGAAAGGAAACCTATCGTTATGATGTCTCTGTTCAGATGATTGAGATTTATAATGAGCAAGTCCGGGATCTCCTCGTCACTGATGGAACTAATAAAAGATATCCTTCATAG

mRNA sequence

ATGGAAAGAACGCTGTCGTTTTCAGTGGCATCTGTGGTGGAGGATGTTCTTCAACAGCATGGGAATCGTCTTGGTAATGGAGGAGGAGGCCTTGATTTGGAATCTAGAAGAGCTGAGGAAGCTGGTAACTCTGCTTTTTCTAATCTTCTTTTTTTCCCAAATCTTTGGAGTCTTGTGAATCTGATGAATGAGTCTGCATCTAGAAGAAACGAAGCTGCTGGATGGCTGAGAAAGATGATCGGGGTTGTTGCCGCTAAAGATTTGCCAGCTGAACCTTCAGAAGAAGAATTCAGGCTTGGATTGAGAAGTGGGATTATTCTCTGCAATGTCCTGAATAAGGTCCAACCAGGAGCAGTGCCCAAGGTGGTTGAAAGTCCTTGTGATTCTGCCCTCATCCCTGATGGAGCTGCACTCTCAGCATTTCAGTATTTTGAGAACGTGAGGAATTTTCTTGTAGCTATTCAGGAGATGGGAGTTCCTACCTTTGAGGCATCTGATCTAGAGCAAGGAGGGACATCTGCCAGAGTAGTCAATACTGTTCTAGCTCTAAAATCTTATGGCGAGTGGAAACAGGGTGGAGGCTATGGAGTTTGGAAATTCGGTGGAAACGTGAAACCTACAACTATGTCCGCTACTAAGTCCTTTGTGAGGAAAAATTCAGAGCCATTCACAAATTCCTTGTCAAGAACCTCATCTCTGAATGACAAAGCCTTCAATTCTTCCAACGTTGAATGGAACAAAACGACTAGTTCTCGCTCCTCGCTCATTCGTGCACTTTTAACGGATAAACGGCCTGAAGAAATCCCAACGTTGGTAGAATCATTGCTAAGCAAGCTTGTGGATGAAGTTGAGAATCGCTTTTCCAGCCTTGAACTGACAAAAGTAAATTCAAAAGATGTTGTTGCTGCTACATCACAAGGAAACAAGTTGCACTTCAAGTCAGCTTTTGGTGCTAAAAGGGCAGAGGAACTTAACTCCAAAGCCACGGAAAAGAATGAAATAATACATGAAAGCAGTATATTTGAGGAGCAATCAAAGAGCCTGCTCCTGAAGCAGCAAACGATTTTCGATCAGCAACAAAAGGATGTTCAGGAACTAAAACATAAGCTGCACGCTGCGAAAGCTGGAATGCAGTTTATGCAAGTAAAATTTAGTGAAGAGTTCCAGAACCTCGGTATGCATGTACATAGTCTTGCTCATGCTGCATCTGGATATCATAAAGTTCTCGAGGAAAACCGTAAGCTATACAACCAAGTGCAGGATCTCAAGGGTAGCATAAGAGTTTACTGCCGAGTTAGACCCTTTTTGTCTGGTCAATCAAATTATTTGAGTGTCGTGGATCACATAGAAGATGGAAATATTACTGTTAACGCCCCCTCAAAGCATGGGAAGGGACAGAGATCATTCAGCTTCAATAAAGTATTTGGTCCATCTGCTACACAAGTGGAGGTCTTTGCTGATATGCAGCCACTCATTCGGTCTGTTCTTGATGGATACAATGTTTGCATTTTGCATATGGACAAACAGGATCAGGGAAAACTTTTACTATGGTATACTGGGCCAAAAGAGCTAACAGAGAAGAGTCAAGGAGTAAATTATAGAGCATTGGGAGATTTGTTCCTTATAGCAGATCAAAGAAAGGAAACCTATCGTTATGATGTCTCTGTTCAGATGATTGAGATTTATAATGAGCAAGTCCGGGATCTCCTCGTCACTGATGGAACTAATAAAAGATATCCTTCATAG

Coding sequence (CDS)

ATGGAAAGAACGCTGTCGTTTTCAGTGGCATCTGTGGTGGAGGATGTTCTTCAACAGCATGGGAATCGTCTTGGTAATGGAGGAGGAGGCCTTGATTTGGAATCTAGAAGAGCTGAGGAAGCTGGTAACTCTGCTTTTTCTAATCTTCTTTTTTTCCCAAATCTTTGGAGTCTTGTGAATCTGATGAATGAGTCTGCATCTAGAAGAAACGAAGCTGCTGGATGGCTGAGAAAGATGATCGGGGTTGTTGCCGCTAAAGATTTGCCAGCTGAACCTTCAGAAGAAGAATTCAGGCTTGGATTGAGAAGTGGGATTATTCTCTGCAATGTCCTGAATAAGGTCCAACCAGGAGCAGTGCCCAAGGTGGTTGAAAGTCCTTGTGATTCTGCCCTCATCCCTGATGGAGCTGCACTCTCAGCATTTCAGTATTTTGAGAACGTGAGGAATTTTCTTGTAGCTATTCAGGAGATGGGAGTTCCTACCTTTGAGGCATCTGATCTAGAGCAAGGAGGGACATCTGCCAGAGTAGTCAATACTGTTCTAGCTCTAAAATCTTATGGCGAGTGGAAACAGGGTGGAGGCTATGGAGTTTGGAAATTCGGTGGAAACGTGAAACCTACAACTATGTCCGCTACTAAGTCCTTTGTGAGGAAAAATTCAGAGCCATTCACAAATTCCTTGTCAAGAACCTCATCTCTGAATGACAAAGCCTTCAATTCTTCCAACGTTGAATGGAACAAAACGACTAGTTCTCGCTCCTCGCTCATTCGTGCACTTTTAACGGATAAACGGCCTGAAGAAATCCCAACGTTGGTAGAATCATTGCTAAGCAAGCTTGTGGATGAAGTTGAGAATCGCTTTTCCAGCCTTGAACTGACAAAAGTAAATTCAAAAGATGTTGTTGCTGCTACATCACAAGGAAACAAGTTGCACTTCAAGTCAGCTTTTGGTGCTAAAAGGGCAGAGGAACTTAACTCCAAAGCCACGGAAAAGAATGAAATAATACATGAAAGCAGTATATTTGAGGAGCAATCAAAGAGCCTGCTCCTGAAGCAGCAAACGATTTTCGATCAGCAACAAAAGGATGTTCAGGAACTAAAACATAAGCTGCACGCTGCGAAAGCTGGAATGCAGTTTATGCAAGTAAAATTTAGTGAAGAGTTCCAGAACCTCGGTATGCATGTACATAGTCTTGCTCATGCTGCATCTGGATATCATAAAGTTCTCGAGGAAAACCGTAAGCTATACAACCAAGTGCAGGATCTCAAGGGTAGCATAAGAGTTTACTGCCGAGTTAGACCCTTTTTGTCTGGTCAATCAAATTATTTGAGTGTCGTGGATCACATAGAAGATGGAAATATTACTGTTAACGCCCCCTCAAAGCATGGGAAGGGACAGAGATCATTCAGCTTCAATAAAGTATTTGGTCCATCTGCTACACAAGTGGAGGTCTTTGCTGATATGCAGCCACTCATTCGGTCTGTTCTTGATGGATACAATGTTTGCATTTTGCATATGGACAAACAGGATCAGGGAAAACTTTTACTATGGTATACTGGGCCAAAAGAGCTAACAGAGAAGAGTCAAGGAGTAAATTATAGAGCATTGGGAGATTTGTTCCTTATAGCAGATCAAAGAAAGGAAACCTATCGTTATGATGTCTCTGTTCAGATGATTGAGATTTATAATGAGCAAGTCCGGGATCTCCTCGTCACTGATGGAACTAATAAAAGATATCCTTCATAG

Protein sequence

MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALKSYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFNSSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKDVVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDLFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKRYPS
Homology
BLAST of Lag0031033 vs. NCBI nr
Match: QWT43339.1 (kinesin-like protein KIN14J [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 979.9 bits (2532), Expect = 9.3e-282
Identity = 518/582 (89.00%), Postives = 528/582 (90.72%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQ+MGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQDMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGG+GVWKFGGNVKP TTMSATKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYGEWKQGGGFGVWKFGGNVKPTTTMSATKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSNVEWNKT SSR+SLIRALLTDKRPEEIP  VESLLSKLVDEVENRFSSLELTK   KD
Sbjct: 241 SSNVEWNKTNSSRASLIRALLTDKRPEEIPMFVESLLSKLVDEVENRFSSLELTKATPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           +VAATSQ NK   KSAFGAKRAE+LNSK  EKNEIIHESSIFEEQSKSLLLKQQ IFDQQ
Sbjct: 301 MVAATSQSNKSLLKSAFGAKRAEDLNSKVMEKNEIIHESSIFEEQSKSLLLKQQVIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKD  ELKHKLHAAKAGMQFMQVKF+EEF NLGMHVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKD--ELKHKLHAAKAGMQFMQVKFNEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIE+GNITVNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEEGNITVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPLIRSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKRYPS 582
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKRYPS
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKRYPS 555

BLAST of Lag0031033 vs. NCBI nr
Match: XP_038906001.1 (kinesin-like protein KIN-14I isoform X2 [Benincasa hispida])

HSP 1 Score: 976.5 bits (2523), Expect = 1.0e-280
Identity = 515/579 (88.95%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNK+QPGAV 
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKIQPGAVS 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKPT-TMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKPT TMS TKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTATMSTTKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN+EWNKT SSR+SLIRALLTDKRPEEIP  VESLLSKLVDEVENRFSSLELTK   KD
Sbjct: 241 SSNIEWNKTNSSRTSLIRALLTDKRPEEIPMFVESLLSKLVDEVENRFSSLELTKTTPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VVAATSQ NK   KSAFGAKRAE+LNSK  EKNEIIH+SSIFEEQSKSLLLKQQ IFDQQ
Sbjct: 301 VVAATSQSNKSLLKSAFGAKRAEDLNSKVMEKNEIIHQSSIFEEQSKSLLLKQQVIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKH+LHAAKAGMQFMQVKFSEEF NLGMHVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKDVQELKHELHAAKAGMQFMQVKFSEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLS QSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSDQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPLIRSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 554

BLAST of Lag0031033 vs. NCBI nr
Match: XP_008454311.2 (PREDICTED: kinesin-4 [Cucumis melo])

HSP 1 Score: 974.5 bits (2518), Expect = 3.9e-280
Identity = 514/579 (88.77%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKP TTMSATKSFVRKNSEPFTNSLSRTSSLNDK FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTTTMSATKSFVRKNSEPFTNSLSRTSSLNDKTFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN +WNKT SSR++LIRALLTDKRPEEIPT VESLLSKLVDEVENRFSSL+LTK   KD
Sbjct: 241 SSNADWNKTNSSRAALIRALLTDKRPEEIPTFVESLLSKLVDEVENRFSSLDLTKATPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VV+A+SQ NK   KSAFGAKRAEE NSK  EKNEIIHESSIFEEQSKSLLLKQQ IFDQQ
Sbjct: 301 VVSASSQSNKSLLKSAFGAKRAEEPNSKTIEKNEIIHESSIFEEQSKSLLLKQQVIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHAAKAGMQFMQVKFSEEF NLGMHVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSNYLSVVD IEDGNITVNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDSIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDG+NKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGSNKR 554

BLAST of Lag0031033 vs. NCBI nr
Match: XP_038906000.1 (kinesin-like protein KIN-14I isoform X1 [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.5e-279
Identity = 515/580 (88.79%), Postives = 524/580 (90.34%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNK+QPGAV 
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKIQPGAVS 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKPT-TMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKPT TMS TKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTATMSTTKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKT-TSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSK 300
           SSN+EWNKT  SSR+SLIRALLTDKRPEEIP  VESLLSKLVDEVENRFSSLELTK   K
Sbjct: 241 SSNIEWNKTQNSSRTSLIRALLTDKRPEEIPMFVESLLSKLVDEVENRFSSLELTKTTPK 300

Query: 301 DVVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQ 360
           DVVAATSQ NK   KSAFGAKRAE+LNSK  EKNEIIH+SSIFEEQSKSLLLKQQ IFDQ
Sbjct: 301 DVVAATSQSNKSLLKSAFGAKRAEDLNSKVMEKNEIIHQSSIFEEQSKSLLLKQQVIFDQ 360

Query: 361 QQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQ 420
           QQKDVQELKH+LHAAKAGMQFMQVKFSEEF NLGMHVHSLAHAASGYHKVLEENRKLYNQ
Sbjct: 361 QQKDVQELKHELHAAKAGMQFMQVKFSEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQ 420

Query: 421 VQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPS 480
           VQDLKGSIRVYCRVRPFLS QSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPS
Sbjct: 421 VQDLKGSIRVYCRVRPFLSDQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPS 480

Query: 481 ATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGD 540
           ATQVEVFADMQPLIRSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGD
Sbjct: 481 ATQVEVFADMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGD 540

Query: 541 LFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           LFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR
Sbjct: 541 LFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 555

BLAST of Lag0031033 vs. NCBI nr
Match: XP_011652945.1 (kinesin-like protein KIN-14I isoform X2 [Cucumis sativus])

HSP 1 Score: 961.8 bits (2485), Expect = 2.6e-276
Identity = 509/579 (87.91%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGN GGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGN-GGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKP TTMSATKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTTTMSATKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN +WNKT SSR++LIRALLTDKRPEEIPT VESLLSKLVDEVENRFSSL+LTK   KD
Sbjct: 241 SSNADWNKTNSSRAALIRALLTDKRPEEIPTFVESLLSKLVDEVENRFSSLDLTKATPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VV+A SQ NK   KSAFGAKRAEE NSKA EKNEI HESSIFEEQSKSLL+KQQ +FDQQ
Sbjct: 301 VVSAGSQSNKSLLKSAFGAKRAEEPNSKAIEKNEITHESSIFEEQSKSLLMKQQAVFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHAAKAGMQFMQVKFSEEF +LG+HVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFHHLGVHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSNYLSVVD+IEDGNI+VNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDNIEDGNISVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPLIRSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDG+NKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGSNKR 553

BLAST of Lag0031033 vs. ExPASy Swiss-Prot
Match: F4IL57 (Kinesin-like protein KIN-14I OS=Arabidopsis thaliana OX=3702 GN=KIN14I PE=2 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.7e-196
Identity = 370/577 (64.12%), Postives = 443/577 (76.78%), Query Frame = 0

Query: 5   LSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNE 64
           LSF+VASV+EDVLQQHGN L +     DL SRRAEEA                       
Sbjct: 11  LSFTVASVMEDVLQQHGNGLRDH----DLVSRRAEEA----------------------- 70

Query: 65  SASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVE 124
            ASRR EAA WLR+M+GVV AKDLPAEP+EE  RLGLRSGIILC VLNKVQPGAV KVVE
Sbjct: 71  -ASRRYEAANWLRRMVGVVGAKDLPAEPTEEGLRLGLRSGIILCKVLNKVQPGAVSKVVE 130

Query: 125 SPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALK 184
           SPCD+ L+ DGA LSAFQYFENVRNFLVAIQEMG PTFEASDLEQGG ++RVVN VLA+K
Sbjct: 131 SPCDAILVADGAPLSAFQYFENVRNFLVAIQEMGFPTFEASDLEQGGNASRVVNCVLAIK 190

Query: 185 SYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFNSSNVE 244
           SY EWKQ GG GVWKFGGN+KP  +  + SFVRKNSEPF NSLSRTSS+N++   S N +
Sbjct: 191 SYDEWKQSGGIGVWKFGGNIKPPALGKS-SFVRKNSEPFMNSLSRTSSINNEKAPSEN-D 250

Query: 245 WNK--TTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSS-LELTKVNSKDVV 304
            NK  + SS S+L+RA+L+DK+PE++P L+ESLLSK+V+E ENR ++  EL +   ++  
Sbjct: 251 SNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESLLSKVVEEFENRVTNQYELVRAAPRE-- 310

Query: 305 AATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQQK 364
            +TS  N   F    G +  EE + KA +K++  H S I +E+ K+   KQ TIF+QQQ+
Sbjct: 311 -STSSQNNRSFLKPLGEREREEKSFKAIKKDD--HNSQILDEKMKTRQFKQLTIFNQQQE 370

Query: 365 DVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQVQD 424
           D++ L+  L+  +AGMQFMQ KF EEF +LGMHVH LAHAASGYH+VLEENRKLYNQVQD
Sbjct: 371 DIEGLRQTLYTTRAGMQFMQKKFQEEFSSLGMHVHGLAHAASGYHRVLEENRKLYNQVQD 430

Query: 425 LKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSATQ 484
           LKGSIRVYCRVRPFL GQS++ S + ++ED  I +N  S+HGK  +SF+FNKVFGPSATQ
Sbjct: 431 LKGSIRVYCRVRPFLPGQSSFSSTIGNMEDDTIGINTASRHGKSLKSFTFNKVFGPSATQ 490

Query: 485 VEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDLFL 544
            EVF+DMQPLIRSVLDGYNVCI    +   GK     +GP++LTEKSQGVNYRALGDLFL
Sbjct: 491 EEVFSDMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPRDLTEKSQGVNYRALGDLFL 550

Query: 545 IADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           +A+QRK+T+RYD++VQMIEIYNEQVRDLLVTDG+NKR
Sbjct: 551 LAEQRKDTFRYDIAVQMIEIYNEQVRDLLVTDGSNKR 551

BLAST of Lag0031033 vs. ExPASy Swiss-Prot
Match: O81635 (Kinesin-like protein KIN-14G OS=Arabidopsis thaliana OX=3702 GN=KIN14G PE=1 SV=2)

HSP 1 Score: 542.3 bits (1396), Expect = 6.5e-153
Identity = 310/578 (53.63%), Postives = 395/578 (68.34%), Query Frame = 0

Query: 5   LSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNE 64
           LSFSV S+VEDVLQQH +R  + G    L SR+ E                        E
Sbjct: 11  LSFSVVSIVEDVLQQHSSRSSDVG----LVSRKVE------------------------E 70

Query: 65  SASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVE 124
           S+ RR EAAGWLR MIGV   KD P EPSEEEFRLGLRSGI+LCNVLNKV PG+V KVVE
Sbjct: 71  SSLRRYEAAGWLRDMIGVSNGKDFPGEPSEEEFRLGLRSGIVLCNVLNKVNPGSVSKVVE 130

Query: 125 SPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALK 184
           +P D   + DGAALSAFQYFEN+RNFLVAI+EMG+P+FEASD+E+GG S R+VN +LALK
Sbjct: 131 APDD---VADGAALSAFQYFENIRNFLVAIEEMGLPSFEASDMEKGGKSIRIVNCILALK 190

Query: 185 SYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFNSSNVE 244
           SY EWK  G  G W++G N+K     + K F+RK+SEPF +S+SRT S  D       + 
Sbjct: 191 SYSEWKLKGENGPWRYGSNMK-HNFGSRKLFLRKSSEPFVSSISRTQS-TDMLSTDQPLS 250

Query: 245 WNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFS-SLELTKVNSKDVVAA 304
            +  + S + L+R+ + D++ E+IP +VES+L+K+++EV+ R S   E+ K +SK +   
Sbjct: 251 SDGDSRSINGLVRSFIADRKHEDIPNVVESVLNKVMEEVQQRLSIHNEMMKSSSKPIPED 310

Query: 305 TSQGNKLHFKSAFGAKRAEELNSKA-TEKNEIIHESSIFEEQSKSLLLKQQTIFDQQQKD 364
            S    +       A++ EE    +  +  E   + + FE        ++Q I   QQK 
Sbjct: 311 DSSCETVVRSQLCDARQHEEAEENSPPQVVEKKFQRTNFEHH------EEQKILLNQQKH 370

Query: 365 VQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQVQDL 424
           +QELK  L+  KAGM+ +Q+K+ E+F +LG H++ LA+AA+GY +VLEENRKLYN VQDL
Sbjct: 371 IQELKQTLYTTKAGMKLLQMKYQEDFFHLGKHLNGLAYAATGYKRVLEENRKLYNLVQDL 430

Query: 425 KGSIRVYCRVRPFLSGQ-SNYLSVVDHIEDGNITVNAPSKHGK-GQRSFSFNKVFGPSAT 484
           KG+IRVYCRVRPFL GQ S  LS V+ I++G IT+  PSK+GK GQ+ F FNKVFGPSAT
Sbjct: 431 KGNIRVYCRVRPFLPGQESGGLSAVEDIDEGTITIRVPSKYGKAGQKPFMFNKVFGPSAT 490

Query: 485 QVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDLF 544
           Q EVF+DMQPL+RSVLDGYNVCI    +   GK     TGPKELTE+S GVNYRAL DLF
Sbjct: 491 QEEVFSDMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MTGPKELTEESLGVNYRALADLF 548

Query: 545 LIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           L+++QRK+T  Y++SVQM+EIYNEQVRDLL  DG  KR
Sbjct: 551 LLSNQRKDTTSYEISVQMLEIYNEQVRDLLAQDGQTKR 548

BLAST of Lag0031033 vs. ExPASy Swiss-Prot
Match: Q10MN5 (Kinesin-like protein KIN-14F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14F PE=2 SV=2)

HSP 1 Score: 531.9 bits (1369), Expect = 8.7e-150
Identity = 320/604 (52.98%), Postives = 388/604 (64.24%), Query Frame = 0

Query: 6   SFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNES 65
           S S A+VVEDVL+QHG RL +     DL SRRAEEA                        
Sbjct: 9   SLSAAAVVEDVLRQHGCRLSD----RDLASRRAEEA------------------------ 68

Query: 66  ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPK---- 125
           A+RRNEAAGWLR+ +G VAA+DLP EPSEEEFRLGLR+G ILC  LN+V PGAVPK    
Sbjct: 69  AARRNEAAGWLRRTVGAVAARDLPEEPSEEEFRLGLRNGQILCGALNRVHPGAVPKACAH 128

Query: 126 --------------------VVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPT 185
                               VV +  DS L PDGAALSAFQYFENVRNFLVA QE+G+P 
Sbjct: 129 VVFVNLIRSRCAVCHCSVMVVVNTAADSVLQPDGAALSAFQYFENVRNFLVAAQEIGLPC 188

Query: 186 FEASDLEQGGTSARVVNTVLALKSYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSE 245
           FEASDLEQGG SARVVN VLALKSYG+WKQ GG G WK+GGN+KP+  ++ KSFVRKNSE
Sbjct: 189 FEASDLEQGGKSARVVNCVLALKSYGDWKQCGGTGPWKYGGNLKPS--ASGKSFVRKNSE 248

Query: 246 PFTNSLSRTSS---LNDKAFNSS-NVEWNKTTSSR--SSLIRALLTDKRPEEIPTLVESL 305
           PF    S         +  F+   +++    ++SR    L+ A+L+DKRP+E+P  V++ 
Sbjct: 249 PFRRCQSMNEGEVPYEEAGFSGDYHLDSGDMSTSRPLKMLVSAVLSDKRPDEVPQ-VKAA 308

Query: 306 LSKLVDEVENRFSSLELTKVNSKDVVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEII 365
           L    D  +    S   +K+++ +V +   Q      K A+G                  
Sbjct: 309 LKNGTDGTK----SFSKSKMDTIEVYSKHRQTK----KEAYG------------------ 368

Query: 366 HESSIFEEQSKSLLLKQQTIFDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHV 425
                       + LKQ ++   Q K V+ELK  + A KAGM+FMQ+K+SE+   LG H+
Sbjct: 369 -----------EVTLKQYSMLQLQSKHVEELKADIRATKAGMEFMQMKYSEDINILGRHL 428

Query: 426 HSLAHAASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNIT 485
            SLAHAASGYH VLEENRKLYNQVQDLKGSIRVYCRVRPFL GQ +  + V  I++GNIT
Sbjct: 429 FSLAHAASGYHIVLEENRKLYNQVQDLKGSIRVYCRVRPFLPGQVSSCA-VGSIDEGNIT 488

Query: 486 VNAPSKHGK-GQRSFSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKL 545
           +  PSK GK G+++FSFNKVFGPSATQ EVF D QPLIRSVLDGYNVCI    +   GK 
Sbjct: 489 IITPSKSGKEGRKTFSFNKVFGPSATQDEVFLDTQPLIRSVLDGYNVCIFAYGQTGSGKT 542

Query: 546 LLWYTGPKELTEKSQGVNYRALGDLFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDG 579
               +GPK +TE++QGVNYRAL DLF +A+QRK  + YD++VQMIEIYNEQVRDLLV DG
Sbjct: 549 YT-MSGPKNMTEQTQGVNYRALSDLFKLAEQRKGAFIYDIAVQMIEIYNEQVRDLLVNDG 542

BLAST of Lag0031033 vs. ExPASy Swiss-Prot
Match: F4HZF0 (Kinesin-like protein KIN-14H OS=Arabidopsis thaliana OX=3702 GN=KIN14H PE=3 SV=2)

HSP 1 Score: 510.4 bits (1313), Expect = 2.7e-143
Identity = 280/534 (52.43%), Postives = 369/534 (69.10%), Query Frame = 0

Query: 62  MNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPK 121
           ++ S  RR EAA W+R  +GVV  +DLPA+PSEE+FR+ LRSGI+LCNVLN+V+PGAVPK
Sbjct: 50  VDPSDLRRYEAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRVKPGAVPK 109

Query: 122 VVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVL 181
           VVE+P D  +  DGAALSAFQYFEN+RNFLV ++EMG+PTFE SD E+GG SAR+V  VL
Sbjct: 110 VVEAPNDPLVNQDGAALSAFQYFENLRNFLVFVEEMGIPTFEVSDFEKGGKSARIVECVL 169

Query: 182 ALKSYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSE----PFTNSLSRTSSLNDKA 241
           ALKSY EWKQ GG G W++  N KPTT    K + RK+SE      TNS S T S     
Sbjct: 170 ALKSYREWKQSGGSGTWRYILNSKPTTFGIAKQYKRKDSEVPVDAVTNSPSSTPSSEQPL 229

Query: 242 F--NSSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSS---LEL 301
              + SN + + T SS  +++RA+ +D + E+IP +VE +L  ++ E E R ++   L L
Sbjct: 230 LDQSDSNTKNDGTASSIDAIVRAVFSDMKQEDIPVIVEDMLKSVMVEYERRLATQNELLL 289

Query: 302 TKVNSKDVVAA-----TSQGNKLHFKSA-FGAKRAEEL---NSKATEKN---EIIHESSI 361
               ++D + +     T  GN+     A +G +   E+   N +A++ +   E+ ++   
Sbjct: 290 MSAGNRDKLGSGDLGRTISGNEETLSDASYGEENVTEIVNNNMEASQDSNVEELENQDYE 349

Query: 362 FEEQSKSLLLKQQTIFDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAH 421
               SK    KQQ I ++QQ   +ELKH L A KAG+  +Q+K+ +EF +LG H+H L +
Sbjct: 350 LYAISKEKTEKQQLIIERQQTHTEELKHDLKAVKAGLSLLQMKYQQEFTSLGKHLHGLTY 409

Query: 422 AASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPS 481
           AA+GY +VLEENRKLYNQVQDLKGSIRVYCRVRPFL GQ + L+ VDH+ED  +++  PS
Sbjct: 410 AATGYQRVLEENRKLYNQVQDLKGSIRVYCRVRPFLPGQKSVLTTVDHLEDSTLSIATPS 469

Query: 482 KHGK-GQRSFSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYT 541
           K+GK GQ++F+FNKVFGPSA+Q  VFAD QPLIRSVLDGYNVCI    +   GK      
Sbjct: 470 KYGKEGQKTFTFNKVFGPSASQEAVFADTQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MM 529

Query: 542 GPKELTEKSQGVNYRALGDLFLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTD 574
           GP ELT+++ GVNYRAL DLF ++  RKET+ Y++SVQM+EIYNEQVRDLL T+
Sbjct: 530 GPNELTDETLGVNYRALSDLFHLSSVRKETFSYNISVQMLEIYNEQVRDLLATN 582

BLAST of Lag0031033 vs. ExPASy Swiss-Prot
Match: B9G8P1 (Kinesin-like protein KIN-14P OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14P PE=3 SV=2)

HSP 1 Score: 498.4 bits (1282), Expect = 1.1e-139
Identity = 299/596 (50.17%), Postives = 388/596 (65.10%), Query Frame = 0

Query: 8   SVASVVEDVLQQHGNRLGNG-------GGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 67
           + A+VVED L+ +G+  G G       G  +D+E R+AEEA                   
Sbjct: 12  ATAAVVEDGLRGNGDGGGGGVGEVVGVGRSIDMEWRKAEEA------------------- 71

Query: 68  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 127
                A RR EAA WLR+++GVV  KDL  EPSEEEFRLGLR+GI+LCN LNKVQPG+VP
Sbjct: 72  -----AIRRYEAANWLRRVVGVVCGKDLAEEPSEEEFRLGLRNGIVLCNALNKVQPGSVP 131

Query: 128 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 187
           KVVE+P DSA   DGAAL A+QYFENVRNFL+ +Q++G+PTFEASDLE+GG   RVV+ V
Sbjct: 132 KVVEAPSDSA---DGAALCAYQYFENVRNFLMGLQDLGLPTFEASDLEKGGKGVRVVDCV 191

Query: 188 LALKSYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSS---LNDKA 247
           L+L+S+ E KQ G     K+GG +KP+   + K F+RKNSEPF  ++ R+ S   L D  
Sbjct: 192 LSLRSFSESKQVGRSAPLKYGGILKPS--MSGKHFIRKNSEPFVKTMVRSYSAELLRDGV 251

Query: 248 ---------FNSSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFS 307
                    F+  +VE   T  S   L++ +L+DK+PEEIP+LVESLLS+++ E E R +
Sbjct: 252 SLEQSLGLDFSLEHVE-RTTPDSIRMLVQTMLSDKKPEEIPSLVESLLSRVIHEFERRTA 311

Query: 308 SLELTKVNSKDVVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHES-SIFEEQSKS 367
           +      N     A     +KL  ++    +            +E  H S S+ EE S +
Sbjct: 312 N-----QNESVKHALDPNDDKLLSRADTPPEMESTCTCSTGNMDEEDHTSVSMKEEVSTA 371

Query: 368 LLLKQQTI------------FDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHV 427
           +L+  + +            FDQQQK +++LK  L   K+GM+ +++++SE+   LG HV
Sbjct: 372 VLVNGENVVEHIQAKQTDKYFDQQQKHIKDLKSNLATMKSGMEHIKLQYSEDLDKLGKHV 431

Query: 428 HSLAHAASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNIT 487
           H+L+HAASGYHKVLEENRKLYNQ+QDL+G+IRVYCRVRPFL G+ +  S V  +ED  IT
Sbjct: 432 HTLSHAASGYHKVLEENRKLYNQIQDLRGNIRVYCRVRPFLPGKVSSSSSVAGLEDRTIT 491

Query: 488 VNAPSKHGK-GQRSFSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKL 547
           V  PSKHGK  ++SF+FN+VFGP ATQ +VFADMQPLIRSVLDGYNVCI    +   GK 
Sbjct: 492 VMTPSKHGKDARKSFTFNRVFGPLATQEQVFADMQPLIRSVLDGYNVCIFAYGQTGSGKT 551

Query: 548 LLWYTGPKELTEKSQGVNYRALGDLFLIADQRKETYRYDVSVQMIEIYNEQVRDLL 571
               +GPK LTE+  GVNYRAL DLF I  QRK+T+ Y++SVQMIEIYNEQVRDLL
Sbjct: 552 FT-MSGPKVLTEEGLGVNYRALNDLFNIQAQRKDTFCYEISVQMIEIYNEQVRDLL 571

BLAST of Lag0031033 vs. ExPASy TrEMBL
Match: A0A1S3BZI8 (kinesin-4 OS=Cucumis melo OX=3656 GN=LOC103494750 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.9e-280
Identity = 514/579 (88.77%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKP TTMSATKSFVRKNSEPFTNSLSRTSSLNDK FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTTTMSATKSFVRKNSEPFTNSLSRTSSLNDKTFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN +WNKT SSR++LIRALLTDKRPEEIPT VESLLSKLVDEVENRFSSL+LTK   KD
Sbjct: 241 SSNADWNKTNSSRAALIRALLTDKRPEEIPTFVESLLSKLVDEVENRFSSLDLTKATPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VV+A+SQ NK   KSAFGAKRAEE NSK  EKNEIIHESSIFEEQSKSLLLKQQ IFDQQ
Sbjct: 301 VVSASSQSNKSLLKSAFGAKRAEEPNSKTIEKNEIIHESSIFEEQSKSLLLKQQVIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHAAKAGMQFMQVKFSEEF NLGMHVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSNYLSVVD IEDGNITVNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDSIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDG+NKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGSNKR 554

BLAST of Lag0031033 vs. ExPASy TrEMBL
Match: A0A5D3E2I5 (Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001280 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.8e-276
Identity = 507/573 (88.48%), Postives = 518/573 (90.40%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSYGEWKQGGGYGVWKFGGNVKP TTMSATKSFVRKNSEPFTNSLSRTSSLNDK FN
Sbjct: 181 LALKSYGEWKQGGGYGVWKFGGNVKPTTTMSATKSFVRKNSEPFTNSLSRTSSLNDKTFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN +WNKT SSR++LIRALLTDKRPEEIPT VESLLSKLVDEVENRFSSL+LTK   KD
Sbjct: 241 SSNADWNKTNSSRAALIRALLTDKRPEEIPTFVESLLSKLVDEVENRFSSLDLTKATPKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VV+A+SQ NK   KSAFGAKRAEE NSK  EKNEIIHESSIFEEQSKSLLLKQQ IFDQQ
Sbjct: 301 VVSASSQSNKSLLKSAFGAKRAEEPNSKTIEKNEIIHESSIFEEQSKSLLLKQQVIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHAAKAGMQFMQVKFSEEF NLGMHVHSLAHAASGYHKVLEENRKLYNQV
Sbjct: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFHNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSNYLSVVD IEDGNITVNAPSKHGKGQRSFSFNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDSIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     +GPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MSGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVT 573
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLL++
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLLS 548

BLAST of Lag0031033 vs. ExPASy TrEMBL
Match: A0A6J1F5H1 (kinesin-like protein KIN-14I isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111442299 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.8e-276
Identity = 508/579 (87.74%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVA+Q+MGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAVQDMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSY EWKQGGGYGVWKFGGNVKP TT+SATKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYDEWKQGGGYGVWKFGGNVKPTTTLSATKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN EWNKTTSSRS L+RALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE  +  SKD
Sbjct: 241 SSNAEWNKTTSSRSMLVRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE--QSCSKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VVAATSQ NK   KSAFGAKRA+EL+SK  EKNEIIHE+S+FE+QSKSLLLKQQ IFDQQ
Sbjct: 301 VVAATSQSNKSLLKSAFGAKRADELHSKEMEKNEIIHENSMFEQQSKSLLLKQQMIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHA KAGMQFMQVKF+EEF NLGMHVHSLAHAASGYHKVLE+NRKLYNQV
Sbjct: 361 QKDVQELKHKLHATKAGMQFMQVKFNEEFHNLGMHVHSLAHAASGYHKVLEDNRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSN LSVVDHIEDGNITVNAPSKHGKGQRSF FNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNGLSVVDHIEDGNITVNAPSKHGKGQRSFGFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     TGPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MTGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 552

BLAST of Lag0031033 vs. ExPASy TrEMBL
Match: A0A6J1F5C4 (kinesin-like protein KIN-14I isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111442299 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.8e-276
Identity = 508/579 (87.74%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVA+Q+MGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAVQDMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKP-TTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSY EWKQGGGYGVWKFGGNVKP TT+SATKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYDEWKQGGGYGVWKFGGNVKPTTTLSATKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN EWNKTTSSRS L+RALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE  +  SKD
Sbjct: 241 SSNAEWNKTTSSRSMLVRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE--QSCSKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VVAATSQ NK   KSAFGAKRA+EL+SK  EKNEIIHE+S+FE+QSKSLLLKQQ IFDQQ
Sbjct: 301 VVAATSQSNKSLLKSAFGAKRADELHSKEMEKNEIIHENSMFEQQSKSLLLKQQMIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHA KAGMQFMQVKF+EEF NLGMHVHSLAHAASGYHKVLE+NRKLYNQV
Sbjct: 361 QKDVQELKHKLHATKAGMQFMQVKFNEEFHNLGMHVHSLAHAASGYHKVLEDNRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQSN LSVVDHIEDGNITVNAPSKHGKGQRSF FNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQSNGLSVVDHIEDGNITVNAPSKHGKGQRSFGFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     TGPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MTGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 552

BLAST of Lag0031033 vs. ExPASy TrEMBL
Match: A0A6J1J0Z5 (kinesin-like protein KIN-14I isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482466 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.4e-275
Identity = 506/579 (87.39%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVN 60
           MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA                   
Sbjct: 1   MERTLSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEA------------------- 60

Query: 61  LMNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120
                ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP
Sbjct: 61  -----ASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVP 120

Query: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTV 180
           KVVESPCDSALIPDGAALSAFQYFENVRNFLVA+Q+MGVPTFEASDLEQGG SARVVNTV
Sbjct: 121 KVVESPCDSALIPDGAALSAFQYFENVRNFLVAVQDMGVPTFEASDLEQGGKSARVVNTV 180

Query: 181 LALKSYGEWKQGGGYGVWKFGGNVKPT-TMSATKSFVRKNSEPFTNSLSRTSSLNDKAFN 240
           LALKSY EWKQGGGYGVWKFGGNVKPT T+SATKSFVRKNSEPFTNSLSRTSSLNDK+FN
Sbjct: 181 LALKSYDEWKQGGGYGVWKFGGNVKPTNTLSATKSFVRKNSEPFTNSLSRTSSLNDKSFN 240

Query: 241 SSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVNSKD 300
           SSN EWNKTTSSRS L+RALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE  +  SKD
Sbjct: 241 SSNAEWNKTTSSRSMLVRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLE--QSCSKD 300

Query: 301 VVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQ 360
           VVAATSQ NK   KS+FGAKRA+EL+SK  EKNEIIHE+S+FE+QSKSLLLKQQ IFDQQ
Sbjct: 301 VVAATSQSNKSLLKSSFGAKRADELHSKEMEKNEIIHENSMFEQQSKSLLLKQQMIFDQQ 360

Query: 361 QKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQV 420
           QKDVQELKHKLHA KAGMQFMQVKF+EEF NLGMHVHSLAHAASGYHKVLE+NRKLYNQV
Sbjct: 361 QKDVQELKHKLHATKAGMQFMQVKFNEEFHNLGMHVHSLAHAASGYHKVLEDNRKLYNQV 420

Query: 421 QDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSA 480
           QDLKGSIRVYCRVRPFLSGQ+N LSVVDHIEDGNITVNAPSKHGKGQRSF FNKVFGPSA
Sbjct: 421 QDLKGSIRVYCRVRPFLSGQANGLSVVDHIEDGNITVNAPSKHGKGQRSFGFNKVFGPSA 480

Query: 481 TQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDL 540
           TQVEVFADMQPL+RSVLDGYNVCI    +   GK     TGPKELTEKSQGVNYRALGDL
Sbjct: 481 TQVEVFADMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MTGPKELTEKSQGVNYRALGDL 540

Query: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR
Sbjct: 541 FLIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 552

BLAST of Lag0031033 vs. TAIR 10
Match: AT2G47500.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 685.6 bits (1768), Expect = 3.4e-197
Identity = 370/577 (64.12%), Postives = 443/577 (76.78%), Query Frame = 0

Query: 5   LSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNE 64
           LSF+VASV+EDVLQQHGN L +     DL SRRAEEA                       
Sbjct: 11  LSFTVASVMEDVLQQHGNGLRDH----DLVSRRAEEA----------------------- 70

Query: 65  SASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVE 124
            ASRR EAA WLR+M+GVV AKDLPAEP+EE  RLGLRSGIILC VLNKVQPGAV KVVE
Sbjct: 71  -ASRRYEAANWLRRMVGVVGAKDLPAEPTEEGLRLGLRSGIILCKVLNKVQPGAVSKVVE 130

Query: 125 SPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALK 184
           SPCD+ L+ DGA LSAFQYFENVRNFLVAIQEMG PTFEASDLEQGG ++RVVN VLA+K
Sbjct: 131 SPCDAILVADGAPLSAFQYFENVRNFLVAIQEMGFPTFEASDLEQGGNASRVVNCVLAIK 190

Query: 185 SYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFNSSNVE 244
           SY EWKQ GG GVWKFGGN+KP  +  + SFVRKNSEPF NSLSRTSS+N++   S N +
Sbjct: 191 SYDEWKQSGGIGVWKFGGNIKPPALGKS-SFVRKNSEPFMNSLSRTSSINNEKAPSEN-D 250

Query: 245 WNK--TTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSS-LELTKVNSKDVV 304
            NK  + SS S+L+RA+L+DK+PE++P L+ESLLSK+V+E ENR ++  EL +   ++  
Sbjct: 251 SNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESLLSKVVEEFENRVTNQYELVRAAPRE-- 310

Query: 305 AATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTIFDQQQK 364
            +TS  N   F    G +  EE + KA +K++  H S I +E+ K+   KQ TIF+QQQ+
Sbjct: 311 -STSSQNNRSFLKPLGEREREEKSFKAIKKDD--HNSQILDEKMKTRQFKQLTIFNQQQE 370

Query: 365 DVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQVQD 424
           D++ L+  L+  +AGMQFMQ KF EEF +LGMHVH LAHAASGYH+VLEENRKLYNQVQD
Sbjct: 371 DIEGLRQTLYTTRAGMQFMQKKFQEEFSSLGMHVHGLAHAASGYHRVLEENRKLYNQVQD 430

Query: 425 LKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPSKHGKGQRSFSFNKVFGPSATQ 484
           LKGSIRVYCRVRPFL GQS++ S + ++ED  I +N  S+HGK  +SF+FNKVFGPSATQ
Sbjct: 431 LKGSIRVYCRVRPFLPGQSSFSSTIGNMEDDTIGINTASRHGKSLKSFTFNKVFGPSATQ 490

Query: 485 VEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDLFL 544
            EVF+DMQPLIRSVLDGYNVCI    +   GK     +GP++LTEKSQGVNYRALGDLFL
Sbjct: 491 EEVFSDMQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MSGPRDLTEKSQGVNYRALGDLFL 550

Query: 545 IADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           +A+QRK+T+RYD++VQMIEIYNEQVRDLLVTDG+NKR
Sbjct: 551 LAEQRKDTFRYDIAVQMIEIYNEQVRDLLVTDGSNKR 551

BLAST of Lag0031033 vs. TAIR 10
Match: AT5G27000.1 (kinesin 4 )

HSP 1 Score: 542.3 bits (1396), Expect = 4.6e-154
Identity = 310/578 (53.63%), Postives = 395/578 (68.34%), Query Frame = 0

Query: 5   LSFSVASVVEDVLQQHGNRLGNGGGGLDLESRRAEEAGNSAFSNLLFFPNLWSLVNLMNE 64
           LSFSV S+VEDVLQQH +R  + G    L SR+ E                        E
Sbjct: 11  LSFSVVSIVEDVLQQHSSRSSDVG----LVSRKVE------------------------E 70

Query: 65  SASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVE 124
           S+ RR EAAGWLR MIGV   KD P EPSEEEFRLGLRSGI+LCNVLNKV PG+V KVVE
Sbjct: 71  SSLRRYEAAGWLRDMIGVSNGKDFPGEPSEEEFRLGLRSGIVLCNVLNKVNPGSVSKVVE 130

Query: 125 SPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALK 184
           +P D   + DGAALSAFQYFEN+RNFLVAI+EMG+P+FEASD+E+GG S R+VN +LALK
Sbjct: 131 APDD---VADGAALSAFQYFENIRNFLVAIEEMGLPSFEASDMEKGGKSIRIVNCILALK 190

Query: 185 SYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKAFNSSNVE 244
           SY EWK  G  G W++G N+K     + K F+RK+SEPF +S+SRT S  D       + 
Sbjct: 191 SYSEWKLKGENGPWRYGSNMK-HNFGSRKLFLRKSSEPFVSSISRTQS-TDMLSTDQPLS 250

Query: 245 WNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFS-SLELTKVNSKDVVAA 304
            +  + S + L+R+ + D++ E+IP +VES+L+K+++EV+ R S   E+ K +SK +   
Sbjct: 251 SDGDSRSINGLVRSFIADRKHEDIPNVVESVLNKVMEEVQQRLSIHNEMMKSSSKPIPED 310

Query: 305 TSQGNKLHFKSAFGAKRAEELNSKA-TEKNEIIHESSIFEEQSKSLLLKQQTIFDQQQKD 364
            S    +       A++ EE    +  +  E   + + FE        ++Q I   QQK 
Sbjct: 311 DSSCETVVRSQLCDARQHEEAEENSPPQVVEKKFQRTNFEHH------EEQKILLNQQKH 370

Query: 365 VQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEENRKLYNQVQDL 424
           +QELK  L+  KAGM+ +Q+K+ E+F +LG H++ LA+AA+GY +VLEENRKLYN VQDL
Sbjct: 371 IQELKQTLYTTKAGMKLLQMKYQEDFFHLGKHLNGLAYAATGYKRVLEENRKLYNLVQDL 430

Query: 425 KGSIRVYCRVRPFLSGQ-SNYLSVVDHIEDGNITVNAPSKHGK-GQRSFSFNKVFGPSAT 484
           KG+IRVYCRVRPFL GQ S  LS V+ I++G IT+  PSK+GK GQ+ F FNKVFGPSAT
Sbjct: 431 KGNIRVYCRVRPFLPGQESGGLSAVEDIDEGTITIRVPSKYGKAGQKPFMFNKVFGPSAT 490

Query: 485 QVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQGVNYRALGDLF 544
           Q EVF+DMQPL+RSVLDGYNVCI    +   GK     TGPKELTE+S GVNYRAL DLF
Sbjct: 491 QEEVFSDMQPLVRSVLDGYNVCIFAYGQTGSGKTFT-MTGPKELTEESLGVNYRALADLF 548

Query: 545 LIADQRKETYRYDVSVQMIEIYNEQVRDLLVTDGTNKR 579
           L+++QRK+T  Y++SVQM+EIYNEQVRDLL  DG  KR
Sbjct: 551 LLSNQRKDTTSYEISVQMLEIYNEQVRDLLAQDGQTKR 548

BLAST of Lag0031033 vs. TAIR 10
Match: AT1G09170.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 470.3 bits (1209), Expect = 2.2e-132
Identity = 261/510 (51.18%), Postives = 345/510 (67.65%), Query Frame = 0

Query: 62  MNESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPK 121
           ++ S  RR EAA W+R  +GVV  +DLPA+PSEE+FR+ LRSGI+LCNVLN+V+PGAVPK
Sbjct: 50  VDPSDLRRYEAARWVRNTLGVVGGRDLPADPSEEDFRIALRSGILLCNVLNRVKPGAVPK 109

Query: 122 VVESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVL 181
           VVE+P D  +  DGAALSAFQYFEN+RNFLV ++EMG+PTFE SD E+GG SAR+V  VL
Sbjct: 110 VVEAPNDPLVNQDGAALSAFQYFENLRNFLVFVEEMGIPTFEVSDFEKGGKSARIVECVL 169

Query: 182 ALKSYGEWKQGGGYGVWKFGGNVKPTTMSATKSFVRKNSE----PFTNSLSRTSSLNDKA 241
           ALKSY EWKQ GG G W++  N KPTT    K + RK+SE      TNS S T S     
Sbjct: 170 ALKSYREWKQSGGSGTWRYILNSKPTTFGIAKQYKRKDSEVPVDAVTNSPSSTPSSEQPL 229

Query: 242 F--NSSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSS---LEL 301
              + SN + + T SS  +++RA+ +D + E+IP +VE +L  ++ E E R ++   L L
Sbjct: 230 LDQSDSNTKNDGTASSIDAIVRAVFSDMKQEDIPVIVEDMLKSVMVEYERRLATQNELLL 289

Query: 302 TKVNSKDVVAA-----TSQGNKLHFKSA-FGAKRAEEL---NSKATEKN---EIIHESSI 361
               ++D + +     T  GN+     A +G +   E+   N +A++ +   E+ ++   
Sbjct: 290 MSAGNRDKLGSGDLGRTISGNEETLSDASYGEENVTEIVNNNMEASQDSNVEELENQDYE 349

Query: 362 FEEQSKSLLLKQQTIFDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAH 421
               SK    KQQ I ++QQ   +ELKH L A KAG+  +Q+K+ +EF +LG H+H L +
Sbjct: 350 LYAISKEKTEKQQLIIERQQTHTEELKHDLKAVKAGLSLLQMKYQQEFTSLGKHLHGLTY 409

Query: 422 AASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHIEDGNITVNAPS 481
           AA+GY +VLEENRKLYNQVQDLKGSIRVYCRVRPFL GQ + L+ VDH+ED  +++  PS
Sbjct: 410 AATGYQRVLEENRKLYNQVQDLKGSIRVYCRVRPFLPGQKSVLTTVDHLEDSTLSIATPS 469

Query: 482 KHGK-GQRSFSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYT 541
           K+GK GQ++F+FNKVFGPSA+Q  VFAD QPLIRSVLDGYNVCI    +   GK      
Sbjct: 470 KYGKEGQKTFTFNKVFGPSASQEAVFADTQPLIRSVLDGYNVCIFAYGQTGSGKTFT-MM 529

Query: 542 GPKELTEKSQGVNYRALGDLFLIADQRKET 550
           GP ELT+++ GVNYRAL DLF ++  R  T
Sbjct: 530 GPNELTDETLGVNYRALSDLFHLSKIRNST 558

BLAST of Lag0031033 vs. TAIR 10
Match: AT3G44730.1 (kinesin-like protein 1 )

HSP 1 Score: 335.1 bits (858), Expect = 1.1e-91
Identity = 217/550 (39.45%), Postives = 312/550 (56.73%), Query Frame = 0

Query: 84  AAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVVESPCDSAL-IPDGAALSAFQ 143
           A + LP +PSE+EF L LR+G+ILCNVLNKV PG+V KVVE+P   A+   DGAA SA Q
Sbjct: 5   AMETLPEKPSEDEFSLALRNGLILCNVLNKVNPGSVLKVVENPITPAIQYADGAAQSAIQ 64

Query: 144 YFENVRNFLVAIQEMGVPTFEASDLEQGGTSARVVNTVLALKSYGEWKQGGGYGVWKFGG 203
           YFEN+RNFL A+++M + TF ASDLE+GG+S +VV+ +L LK + EWKQ GG GVW++GG
Sbjct: 65  YFENMRNFLKAVEDMQLLTFGASDLEKGGSSNKVVDCILCLKGFYEWKQAGGVGVWRYGG 124

Query: 204 NVKPTTMSATKSFVRKNSEPFTNSLSRTSSLNDKA-------------------FNSSNV 263
            V+        SF RK S P    +   S+ ++                      N  + 
Sbjct: 125 TVR------IVSFNRKGSSPPQYGIGSESTTDESVSLDESESSQYDQLLDFLHLSNEISA 184

Query: 264 EWNKTTSSRS--------SLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKV 323
           E ++T  S +         L+   L +        L E ++  L++ V   FS++     
Sbjct: 185 EESETAISLAFLFDHFALQLLHGYLKESDGINDMPLNEMVIDTLLNRVVKDFSAI----- 244

Query: 324 NSKDVVAATSQGNKLHFKSAFGAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLKQQTI 383
                    SQG +L    +F  ++  + ++    ++E +     + +  K L+ K+ + 
Sbjct: 245 -------LVSQGAQL---GSF-LRKILKCDNGDLSRSEFLAAVFRYLQHRKDLVSKEFSK 304

Query: 384 FDQ-------------------------QQKDVQELKHKLHAAKAGMQFMQVKFSEEFQN 443
           F +                         QQK+++E+K      ++ ++ MQ ++ +E Q 
Sbjct: 305 FCKCGGKLEFSRLNAREFSPGHVEAIGLQQKELEEVKSNFVETRSQVKQMQSEWQKELQR 364

Query: 444 LGMHVHSLAHAASGYHKVLEENRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHI- 503
           +  HV ++   +S YHKVLEENR LYN+VQDLKG+IRVYCRVRPF   Q +  S VD+I 
Sbjct: 365 IVHHVKAMEVTSSSYHKVLEENRLLYNEVQDLKGTIRVYCRVRPFFQEQKDMQSTVDYIG 424

Query: 504 EDGNITVNAPSKHGKGQRS-FSFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDK 563
           E+GNI +N P K  K  R  FSFNKVFG + +Q +++ D QP+IRSVLDG+NVCI    +
Sbjct: 425 ENGNIIINNPFKQEKDARKIFSFNKVFGQTVSQEQIYIDTQPVIRSVLDGFNVCIFAYGQ 484

Query: 564 QDQGKLLLWYTGPKELTEKSQGVNYRALGDLFLIADQRKETYRYDVSVQMIEIYNEQVRD 579
              GK     +GP  +TE + GVNYRAL DLF +++ R     Y++ VQMIEIYNEQVRD
Sbjct: 485 TGSGKTYT-MSGPDLMTETTWGVNYRALRDLFQLSNARTHVVTYEIGVQMIEIYNEQVRD 531

BLAST of Lag0031033 vs. TAIR 10
Match: AT3G10310.1 (P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin Homology) domain )

HSP 1 Score: 260.0 bits (663), Expect = 4.6e-69
Identity = 188/493 (38.13%), Postives = 270/493 (54.77%), Query Frame = 0

Query: 64  ESASRRNEAAGWLRKMIGVVAAKDLPAEPSEEEFRLGLRSGIILCNVLNKVQPGAVPKVV 123
           E+A+RR +A  WL+ ++G +    +P +PSE+EF   LR+G+ILCN +NK+ PGAV KVV
Sbjct: 17  EAAARRFQAVQWLKSVVGQLG---IPNQPSEKEFISCLRNGMILCNAINKIHPGAVSKVV 76

Query: 124 ESPCDSALIPDGAALSAFQYFENVRNFLVAIQEMGVPTFEASDLE----QGGTSARVVNT 183
           E+   S L  +     A+QYFENVRNFLVA++ + +P FEASDLE    + G+  +VV+ 
Sbjct: 77  EN--YSYLNGEYQLPPAYQYFENVRNFLVALETLRLPGFEASDLEKDNLESGSVTKVVDC 136

Query: 184 VLALKSYGEWK-QGGGYGVWKFGGNVKPTT--MSATKSFVRKNSEPFTNSLSRTSSLNDK 243
           +L LK+Y E K    G G++K   +VK  T  +SATK          T S S+TS   D 
Sbjct: 137 ILGLKAYHECKLPSNGNGLYK---HVKTPTFQLSATKIHP-------TLSASKTSRHLDM 196

Query: 244 AFNSSNVEWNKTTSSRSSLIRALLTDKRPEEIPTLVESLLSKLVDEVENRFSSLELTKVN 303
              SS  E N  T   S  ++          I  L    +    + ++    SLE    N
Sbjct: 197 ---SSVRERNDCTDGESDKLKG---------IAKLFADHIFSSKENIDENLVSLENGSEN 256

Query: 304 SK---DVVAATSQGNKLHFKSAF--GAKRAEELNSKATEKNEIIHESSIFEEQSKSLLLK 363
           S+   + + +     +  FK+    G  +  +L S   E+  +  E       S      
Sbjct: 257 SRANFEKILSRFPELQSVFKNLLSEGTLKPSDLKSMPLEELPVHEEDQSSRSLSHKTKCN 316

Query: 364 QQTIFDQQQKDVQELKHKLHAAKAGMQFMQVKFSEEFQNLGMHVHSLAHAASGYHKVLEE 423
            + +   Q+K++  LK+     K   +  QV    +   LG  +  ++ AA GY+KV+EE
Sbjct: 317 HKRLLKTQEKELAVLKNLFIKTKQDFKEFQVYLQRDLMELGNQMQEMSSAAQGYYKVVEE 376

Query: 424 NRKLYNQVQDLKGSIRVYCRVRPFLSGQSNYLSVVDHI-EDGNITVNAPSKHGK-GQRSF 483
           NRKLYN VQDLKG+IRVYCRVRP  + + +   V+D+I +DG++ V  PSK  K  +++F
Sbjct: 377 NRKLYNMVQDLKGNIRVYCRVRPIFNSEMD--GVIDYIGKDGSLFVLDPSKPYKDARKTF 436

Query: 484 SFNKVFGPSATQVEVFADMQPLIRSVLDGYNVCILHMDKQDQGKLLLWYTGPKELTEKSQ 543
            FN+VFGP+ATQ +VF + QPLIRSV+DGYNVCI    +   GK     +GP   +    
Sbjct: 437 QFNQVFGPTATQDDVFRETQPLIRSVMDGYNVCIFAYGQTGSGKTYT-MSGPPGRSATEM 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
QWT43339.19.3e-28289.00kinesin-like protein KIN14J [Citrullus lanatus subsp. vulgaris][more]
XP_038906001.11.0e-28088.95kinesin-like protein KIN-14I isoform X2 [Benincasa hispida][more]
XP_008454311.23.9e-28088.77PREDICTED: kinesin-4 [Cucumis melo][more]
XP_038906000.12.5e-27988.79kinesin-like protein KIN-14I isoform X1 [Benincasa hispida][more]
XP_011652945.12.6e-27687.91kinesin-like protein KIN-14I isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
F4IL574.7e-19664.12Kinesin-like protein KIN-14I OS=Arabidopsis thaliana OX=3702 GN=KIN14I PE=2 SV=1[more]
O816356.5e-15353.63Kinesin-like protein KIN-14G OS=Arabidopsis thaliana OX=3702 GN=KIN14G PE=1 SV=2[more]
Q10MN58.7e-15052.98Kinesin-like protein KIN-14F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14F ... [more]
F4HZF02.7e-14352.43Kinesin-like protein KIN-14H OS=Arabidopsis thaliana OX=3702 GN=KIN14H PE=3 SV=2[more]
B9G8P11.1e-13950.17Kinesin-like protein KIN-14P OS=Oryza sativa subsp. japonica OX=39947 GN=KIN14P ... [more]
Match NameE-valueIdentityDescription
A0A1S3BZI81.9e-28088.77kinesin-4 OS=Cucumis melo OX=3656 GN=LOC103494750 PE=3 SV=1[more]
A0A5D3E2I52.8e-27688.48Kinesin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001280 PE=... [more]
A0A6J1F5H12.8e-27687.74kinesin-like protein KIN-14I isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1F5C42.8e-27687.74kinesin-like protein KIN-14I isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1J0Z51.4e-27587.39kinesin-like protein KIN-14I isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482... [more]
Match NameE-valueIdentityDescription
AT2G47500.13.4e-19764.12P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
AT5G27000.14.6e-15453.63kinesin 4 [more]
AT1G09170.12.2e-13251.18P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
AT3G44730.11.1e-9139.45kinesin-like protein 1 [more]
AT3G10310.14.6e-6938.13P-loop nucleoside triphosphate hydrolases superfamily protein with CH (Calponin ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001715Calponin homology domainSMARTSM00033ch_5coord: 68..183
e-value: 7.0E-13
score: 58.8
IPR001715Calponin homology domainPFAMPF00307CHcoord: 67..188
e-value: 7.2E-17
score: 61.5
IPR001715Calponin homology domainPROSITEPS50021CHcoord: 66..188
score: 19.491983
IPR001752Kinesin motor domainSMARTSM00129kinesin_4coord: 423..581
e-value: 7.4E-8
score: -101.3
IPR001752Kinesin motor domainPROSITEPS50067KINESIN_MOTOR_2coord: 425..581
score: 33.0378
IPR036872CH domain superfamilyGENE3D1.10.418.10coord: 60..188
e-value: 1.8E-30
score: 107.3
IPR036872CH domain superfamilySUPERFAMILY47576Calponin-homology domain, CH-domaincoord: 68..202
IPR036961Kinesin motor domain superfamilyGENE3D3.40.850.10Kinesin motor domaincoord: 367..580
e-value: 7.1E-41
score: 142.3
IPR031852Spindle pole body-associated protein Vik1/Cik1, microtubule binding domainPFAMPF16796Microtub_bdcoord: 411..570
e-value: 2.8E-32
score: 111.8
NoneNo IPR availablePANTHERPTHR47972:SF24P-LOOP NUCLEOSIDE TRIPHOSPHATE HYDROLASE SUPERFAMILY PROTEINcoord: 63..579
NoneNo IPR availablePANTHERPTHR47972KINESIN-LIKE PROTEIN KLP-3coord: 63..579
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 386..577

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031033.1Lag0031033.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007018 microtubule-based movement
molecular_function GO:0005524 ATP binding
molecular_function GO:0008017 microtubule binding
molecular_function GO:0003777 microtubule motor activity
molecular_function GO:0005515 protein binding