Lag0031013 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031013
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionEpoxide hydrolase 2
Locationchr11: 3846659 .. 3848139 (+)
RNA-Seq ExpressionLag0031013
SyntenyLag0031013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTCATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACTGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGATATCGAGCCATCGCGCCGGATCTCCGCGGCTTTGGCGACACCGACGCGCCGGAATCGCACACTTCCTACACGATGCTGCATATCGTCGGCGATTTGGTTGGGGCTTTGGATGTGCTTGGGATTGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGGTCGTCGGGTGGTACTTTTGCTTGTTTAGGCCTGATCGAATCAAGGCGTTTGTGAATTTGAGTGTCCAGTTCTTACCCAGGAATCCTGCAATTTCTTTTGTTGAGGGTTTCAGAGCTGCGTTGGGTGATGACTTCTACATGTGCAGGTTTCAGGTGAATACCGATTGTGAATTTTGTTTTGATTTGCTTAATTCATGAGAAAGGTTTAAAGTACTGTTGTTCGTTCCATTCTGGTCCCAATTTCGTTTGCTTTCAATTGTTTTCTCTCTGTCATATTTTAGCCTTTCTATGACCCGTTTAATAAACATTTCGTTTTTTTTTGTTTTTTTCTATTTAATAAACTCTCAACGTAAGTGTTGATTAATTTCTAACAGGTTCCAGGGGAGGCAGAAAAAGATTTTGCCTCCATTGACACAACTCTGTTGTTCAAGAAATTTTTATGTGGCAGAGATCCAAATCCTCTAATATTGCCTAAAGAGGTTGGAATTCGTTCAATCCCGTCTCCGGAAAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACCATTTTGCTACCAAATTTAAGCAGACAGGCTTCACTGGAGGATTGAACTACTATCGAGCTTTAGACCTGTAAGTTGTAGTTACATCTGATTTAACCATTCATATTACTCTGATTCAACACTCTTTCTCAGTTATAAGTTCAATAATGATTCTATACAACCATCATGTGTTGTTCTAATGGTCAATAAGAACCATGGAAATAATAAAAGGGATTAAAGGGAATGAGTTCAATCCAAATATCAAAGGTAGTTGTCTTGTGAAATTAGTTGAGGGACGCACAAGCTGCCCCAAACATGTTTTGTTGATTCAGGTGAAACCTCATTGTCCTGTTGTAAATGGTTATTTGGATGTATGAAGGTGAAATGATGTTTGTTGCAGAAATTGGGAGCTAACTGCACCGTGGACGAGAGTGCAGGTAGGGGTGCCGGTGAAGTTCGTTGTCGGGGATTTGGATCTGACCTACCATTTGCCAGGAGCCAAGGAATATGTCCACAATGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTAGTTGTAATGGAAGATACTGGCCACTTCATCAACCAAGAAAGACCACATGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTCTGA

mRNA sequence

ATGGACGTCATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACTGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGATATCGAGCCATCGCGCCGGATCTCCGCGGCTTTGGCGACACCGACGCGCCGGAATCGCACACTTCCTACACGATGCTGCATATCGTCGGCGATTTGGTTGGGGCTTTGGATGTGCTTGGGATTGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGGTCGTCGGGTGGTACTTTTGCTTGTTTAGGCCTGATCGAATCAAGGCGTTTGTGAATTTGAGTGTCCAGTTCTTACCCAGGAATCCTGCAATTTCTTTTGTTGAGGGTTTCAGAGCTGCGTTGGGTGATGACTTCTACATGTGCAGGTTTCAGGTTCCAGGGGAGGCAGAAAAAGATTTTGCCTCCATTGACACAACTCTGTTGTTCAAGAAATTTTTATGTGGCAGAGATCCAAATCCTCTAATATTGCCTAAAGAGGTTGGAATTCGTTCAATCCCGTCTCCGGAAAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACCATTTTGCTACCAAATTTAAGCAGACAGGCTTCACTGGAGGATTGAACTACTATCGAGCTTTAGACCTAAATTGGGAGCTAACTGCACCGTGGACGAGAGTGCAGGTAGGGGTGCCGGTGAAGTTCGTTGTCGGGGATTTGGATCTGACCTACCATTTGCCAGGAGCCAAGGAATATGTCCACAATGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTAGTTGTAATGGAAGATACTGGCCACTTCATCAACCAAGAAAGACCACATGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTCTGA

Coding sequence (CDS)

ATGGACGTCATTGAACACAGCACCATCAACACCAATGGCATCAAAATGCACATCGCCTCCATAGGCACTGGCCCCGTCGTTCTTCTTCTCCATGGCTTCCCCGAGCTCTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGATATCGAGCCATCGCGCCGGATCTCCGCGGCTTTGGCGACACCGACGCGCCGGAATCGCACACTTCCTACACGATGCTGCATATCGTCGGCGATTTGGTTGGGGCTTTGGATGTGCTTGGGATTGAGAAGGTGTTTCTGGTAGGGCATGATTGGGGAGCGGTCGTCGGGTGGTACTTTTGCTTGTTTAGGCCTGATCGAATCAAGGCGTTTGTGAATTTGAGTGTCCAGTTCTTACCCAGGAATCCTGCAATTTCTTTTGTTGAGGGTTTCAGAGCTGCGTTGGGTGATGACTTCTACATGTGCAGGTTTCAGGTTCCAGGGGAGGCAGAAAAAGATTTTGCCTCCATTGACACAACTCTGTTGTTCAAGAAATTTTTATGTGGCAGAGATCCAAATCCTCTAATATTGCCTAAAGAGGTTGGAATTCGTTCAATCCCGTCTCCGGAAAACCTTCCTTCTTGGCTGACAGAAGAAGATATCAACCATTTTGCTACCAAATTTAAGCAGACAGGCTTCACTGGAGGATTGAACTACTATCGAGCTTTAGACCTAAATTGGGAGCTAACTGCACCGTGGACGAGAGTGCAGGTAGGGGTGCCGGTGAAGTTCGTTGTCGGGGATTTGGATCTGACCTACCATTTGCCAGGAGCCAAGGAATATGTCCACAATGGCGGATTCAAAAAGGATGTGCCGTTTCTGGAGGAAGTAGTTGTAATGGAAGATACTGGCCACTTCATCAACCAAGAAAGACCACATGAAATCAGTACTCACATCCATGACTTCATCAAGAAATTCTGA

Protein sequence

MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGRDPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELTAPWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQERPHEISTHIHDFIKKF
Homology
BLAST of Lag0031013 vs. NCBI nr
Match: XP_022934345.1 (uncharacterized protein LOC111441538 [Cucurbita moschata] >KAG6581272.1 hypothetical protein SDJN03_21274, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017999.1 ephA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 270/316 (85.44%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GDT++PESHTSYT+LHIVGDLVGALD LGIEKVF+VGHDWGA++ WYFCLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSVQF+PRNPAI FVEGFR+A GDDFY+CRFQVPGEAE+DFASIDT  LFKKFLC R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
             +P  LPK VG+RSIPSPENLPSWLTE+DIN+FAT FK+TGFTGGLNYYRA DL WELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTEKDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A WT+ QV VPVKF++GDLDLTY  PGAKEY+HNGGFKKDVPFLEEV+VM+DT HFINQE
Sbjct: 241 ASWTKAQVRVPVKFIIGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

BLAST of Lag0031013 vs. NCBI nr
Match: XP_023528763.1 (uncharacterized protein LOC111791601 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.0 bits (1525), Expect = 3.0e-165
Identity = 271/316 (85.76%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GDT++PESHTSYT+LHIVGDLVGALD LGIEKVFLVGHDWGA++ WY CLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYLCLFRPDRIKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSVQF+PRNPAI FVEGFR+A GDDFY+CRFQVPGEAE+DFASIDT  LFKKFLC R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
             +P  LPK VG+RSIPSPENLPSWLTE DIN+FAT FK+TGFTGGLNYYRA DL WELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTENDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A WT+ QV VPVKF+VGDLDLTY  PGAKEY+HNGGFKKDVPFLEEV+VM+DT HFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

BLAST of Lag0031013 vs. NCBI nr
Match: XP_022984060.1 (uncharacterized protein LOC111482484 [Cucurbita maxima])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 270/316 (85.44%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GDT++PE HTSYT+LHIVGDLVGALD LGIEKVFLVGHDWGA++ WYFCLFRPDRIKA
Sbjct: 61  GYGDTESPELHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSVQF+PRNPAI FVEGFR+A GDDFY+CRFQVPGEAE+DFASIDT  LFKKFLC R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
             +P  LPK VG+RSIPSPE LPSWLTEEDIN+FAT FK+TGFTGGLNYYRA DL WELT
Sbjct: 181 RSDPPELPKGVGVRSIPSPEKLPSWLTEEDINYFATIFKRTGFTGGLNYYRAFDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A WT+ QV VPVKF+VGDLDLTY  PGAKEY+HNGGFKKDVPFLEEV++M+DT HFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVILMKDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

BLAST of Lag0031013 vs. NCBI nr
Match: XP_004152245.1 (uncharacterized protein LOC101205063 [Cucumis sativus] >KGN52816.1 hypothetical protein Csa_015229 [Cucumis sativus])

HSP 1 Score: 587.8 bits (1514), Expect = 5.6e-164
Identity = 268/316 (84.81%), Postives = 288/316 (91.14%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   METIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           GFGDTDAPESHT+YT  HIVGDLVGALD LGIEKVF+VGHDWGA++ WYFCLFRPDR+KA
Sbjct: 61  GFGDTDAPESHTAYTAFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRVKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSV FLPRNPAISFV+GFR A GDDFY+CRFQVPGEAEKDF SIDTT LFKK LCGR
Sbjct: 121 LVNLSVHFLPRNPAISFVQGFRFAFGDDFYICRFQVPGEAEKDFGSIDTTELFKKILCGR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
            P+P ILPK +GIRSIPSP +LPSWL++EDIN+FATKF  TGFTGG NYYRALDL WELT
Sbjct: 181 TPDPPILPKGLGIRSIPSPHDLPSWLSQEDINYFATKFNHTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A W + QV VPVKF+VGDLDLTY+ PGAKEY+HNGGFK DVPFLEEVVVMEDT HFINQE
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYYFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEIS HIH+FIKKF
Sbjct: 301 RPHEISVHIHEFIKKF 316

BLAST of Lag0031013 vs. NCBI nr
Match: XP_008454330.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >TYK29511.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 587.0 bits (1512), Expect = 9.5e-164
Identity = 268/316 (84.81%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TINTNGI MHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MEAIEHTTINTNGINMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           GFGDTDAPESHT YT  HIVGDLVGALD LGIEKVF+VGHDWGA++ WY CLFRPDR+KA
Sbjct: 61  GFGDTDAPESHTVYTPFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYLCLFRPDRVKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSV FL RNP ISFV+GFR A GDDFY+CRFQVPGEAEKDF SIDT  LFKKFLCGR
Sbjct: 121 LVNLSVHFLRRNPTISFVQGFRIAFGDDFYICRFQVPGEAEKDFGSIDTAELFKKFLCGR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
            P+P ILPKE GIRSIPSP+NLPSWL++EDIN+FATKF +TGFTGG NYYRALDL WELT
Sbjct: 181 SPDPPILPKEFGIRSIPSPQNLPSWLSQEDINYFATKFNRTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A W + QV VPVKF+VGDLDLTYH PGAKEY+HNGGFK DVPFLEEVVVMEDT HFINQE
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYHFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEIS HIHDFIKKF
Sbjct: 301 RPHEISAHIHDFIKKF 316

BLAST of Lag0031013 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.6e-57
Identity = 118/318 (37.11%), Postives = 179/318 (56.29%), Query Frame = 0

Query: 9   INTNGIKMHIASIGT--GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTD 68
           ++TNG+++ +   G    PVV+L HGFPEL YSWRHQ+  L+  GY  +APD RG+G + 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSV 128
            PE+  +Y +  +  DLVG LD +G E+   VGHDWGAVV W   L   DR+ A   LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 QFLPRNPAISFVEGFRAALGDD-FYMCRFQVPGEAEKDFASIDTTLLFKKFLCGRDP--- 188
             LPR   +   + FR+  G++ FY+  FQ PG A+ +    D     ++ + G  P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNG-DPARTMRRMIGGLRPPGD 188

Query: 189 ----NPLILPKEVG-IRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNW 248
                 ++ P   G I  +P P  LP+W+++E+++H+  +F +TGFTGGLN+YR  D NW
Sbjct: 189 QSAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNW 248

Query: 249 ELTAPWTRVQVGVPVKFVVGDLD--LTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGH 308
           E TA      + VP  F+ G  D  LT+        V +G +++        V+++  GH
Sbjct: 249 ETTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISGPYRE--------VLIDGAGH 308

Query: 309 FINQERPHEISTHIHDFI 314
           ++ QERP E++  + +F+
Sbjct: 309 WLQQERPGEVTAALLEFL 316

BLAST of Lag0031013 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 4.7e-49
Identity = 100/297 (33.67%), Postives = 165/297 (55.56%), Query Frame = 0

Query: 14  IKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTDAPESHTS 73
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++G+G++ AP     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSVQFLPRNP 133
           Y M  +  ++V  LD LG+ +   +GHDWG ++ WY  LF P+R++A  +L+  F+P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 AISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGRDPNPLILPK--EV 193
            +S +E  +A    D Y   FQ PG AE +    + +  FK      D + L + K  E 
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GIRSIPSPE--NLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELTAPWTRVQVG 253
           G   + SPE  +L   +TEE+I  +  +FK++GF G LN+YR ++ NW+        ++ 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQERPHEIS 307
           +P   V  + D    +P   +++ +      +P L+   + ED GH+   ++P E++
Sbjct: 488 IPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKRGHI-EDCGHWTQMDKPTEVN 535

BLAST of Lag0031013 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 102/307 (33.22%), Postives = 175/307 (57.00%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTDAPESHT 72
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++G+G++ AP    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSVQFLPRN 132
            Y++  +  D+V  L+ LG+ +   +GHDWG V+ W   LF P+R++A  +L+  F+P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PAISFVEGFRAALGDDFYMCRFQVPGEAEKDF-ASIDTTLLFKKFLCGRDPNPLIL--PK 192
           P +S +E  +A    D Y   FQ PG AE +   ++D T  FK F    D   L     +
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAELEQNLDRT--FKNFFRAHDETFLTTNRVR 426

Query: 193 EVGIRSIPSPE--NLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELTAPWTRVQ 252
           E+G   + +PE  +L   +TEEDI  +  +FK++GF G LN+YR ++ NW+     +  +
Sbjct: 427 ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRK 486

Query: 253 VGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQERPHEIST 312
           + +P   V  + DL  H P   +++ N      +P L+   + +D GH+   ++P E++ 
Sbjct: 487 ILIPALMVTAENDLVLH-PKMSKHMENW-----IPHLKRGHI-KDCGHWTQIDKPAELNR 543

Query: 313 HIHDFIK 315
            + ++++
Sbjct: 547 ILIEWLE 543

BLAST of Lag0031013 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-47
Identity = 101/299 (33.78%), Postives = 162/299 (54.18%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTDAPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++G+GD+ +P    
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSVQFLPRN 132
            Y M  +  ++V  LD LGI +   +GHDW  V+ W   LF P+R++A  +L+  F+P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGRDPNPLIL---PK 192
           P +S ++  R ++    Y   FQ PG AE +    + +  FK F    D    I      
Sbjct: 365 PDVSPMKVIR-SIPVFNYQLYFQEPGVAEAELEK-NMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 EVGIRSIPSPE--NLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELTAPWTRVQ 252
           E+G   + +PE  NL    TEE+I  +  +FK+TGF G LN+YR  + NW+ +      +
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 VGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQERPHEIS 307
           + VP   V  + D+      +K        +K +PFL+   + ED GH+   E+P E++
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHI-EDCGHWTQIEKPTEVN 534

BLAST of Lag0031013 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-45
Identity = 99/307 (32.25%), Postives = 167/307 (54.40%), Query Frame = 0

Query: 13  GIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGFGDTDAPESHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++G+GD+ +P    
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKAFVNLSVQFLPRN 132
            Y M  +  ++V  L+ LGI +   +GHDW  V+ W   LF P+R++A  +L+   +P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGRDPNPLIL---PK 192
           P +S +E  R ++    Y   FQ PG AE +    + +  FK F    D   L+      
Sbjct: 365 PEVSPMEVIR-SIPVFNYQLYFQEPGVAEAELEK-NMSRTFKSFFRTSDDMGLLTVNKAT 424

Query: 193 EVGIRSIPSPEN--LPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELTAPWTRVQ 252
           E+G   + +PE+  +    TEE+I ++  +FK++GF G LN+YR  + NW+ +      +
Sbjct: 425 EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRK 484

Query: 253 VGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQERPHEIST 312
           + VP   V  + D+    P   + + N      +PFL+   + ED GH+   E+P E++ 
Sbjct: 485 ILVPALMVTAEKDIVLR-PEMSKNMENW-----IPFLKRGHI-EDCGHWTQIEKPAEVNQ 542

Query: 313 HIHDFIK 315
            +  ++K
Sbjct: 545 ILIKWLK 542

BLAST of Lag0031013 vs. ExPASy TrEMBL
Match: A0A6J1F7F3 (uncharacterized protein LOC111441538 OS=Cucurbita moschata OX=3662 GN=LOC111441538 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.4e-166
Identity = 270/316 (85.44%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GDT++PESHTSYT+LHIVGDLVGALD LGIEKVF+VGHDWGA++ WYFCLFRPDRIKA
Sbjct: 61  GYGDTESPESHTSYTVLHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSVQF+PRNPAI FVEGFR+A GDDFY+CRFQVPGEAE+DFASIDT  LFKKFLC R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
             +P  LPK VG+RSIPSPENLPSWLTE+DIN+FAT FK+TGFTGGLNYYRA DL WELT
Sbjct: 181 RSDPPKLPKGVGVRSIPSPENLPSWLTEKDINYFATTFKRTGFTGGLNYYRAFDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A WT+ QV VPVKF++GDLDLTY  PGAKEY+HNGGFKKDVPFLEEV+VM+DT HFINQE
Sbjct: 241 ASWTKAQVRVPVKFIIGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVIVMKDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

BLAST of Lag0031013 vs. ExPASy TrEMBL
Match: A0A6J1J7M9 (uncharacterized protein LOC111482484 OS=Cucurbita maxima OX=3661 GN=LOC111482484 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.1e-165
Identity = 270/316 (85.44%), Postives = 291/316 (92.09%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MD IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GDT++PE HTSYT+LHIVGDLVGALD LGIEKVFLVGHDWGA++ WYFCLFRPDRIKA
Sbjct: 61  GYGDTESPELHTSYTVLHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPDRIKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSVQF+PRNPAI FVEGFR+A GDDFY+CRFQVPGEAE+DFASIDT  LFKKFLC R
Sbjct: 121 LVNLSVQFIPRNPAIPFVEGFRSAFGDDFYICRFQVPGEAEEDFASIDTAELFKKFLCSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
             +P  LPK VG+RSIPSPE LPSWLTEEDIN+FAT FK+TGFTGGLNYYRA DL WELT
Sbjct: 181 RSDPPELPKGVGVRSIPSPEKLPSWLTEEDINYFATIFKRTGFTGGLNYYRAFDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A WT+ QV VPVKF+VGDLDLTY  PGAKEY+HNGGFKKDVPFLEEV++M+DT HFINQE
Sbjct: 241 ASWTKAQVRVPVKFIVGDLDLTYQFPGAKEYIHNGGFKKDVPFLEEVILMKDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEISTHIHDFIKKF
Sbjct: 301 RPHEISTHIHDFIKKF 316

BLAST of Lag0031013 vs. ExPASy TrEMBL
Match: A0A0A0KY86 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001830 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.7e-164
Identity = 268/316 (84.81%), Postives = 288/316 (91.14%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   METIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           GFGDTDAPESHT+YT  HIVGDLVGALD LGIEKVF+VGHDWGA++ WYFCLFRPDR+KA
Sbjct: 61  GFGDTDAPESHTAYTAFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRVKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSV FLPRNPAISFV+GFR A GDDFY+CRFQVPGEAEKDF SIDTT LFKK LCGR
Sbjct: 121 LVNLSVHFLPRNPAISFVQGFRFAFGDDFYICRFQVPGEAEKDFGSIDTTELFKKILCGR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
            P+P ILPK +GIRSIPSP +LPSWL++EDIN+FATKF  TGFTGG NYYRALDL WELT
Sbjct: 181 TPDPPILPKGLGIRSIPSPHDLPSWLSQEDINYFATKFNHTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A W + QV VPVKF+VGDLDLTY+ PGAKEY+HNGGFK DVPFLEEVVVMEDT HFINQE
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYYFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEIS HIH+FIKKF
Sbjct: 301 RPHEISVHIHEFIKKF 316

BLAST of Lag0031013 vs. ExPASy TrEMBL
Match: A0A5D3E1N5 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001130 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 268/316 (84.81%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TINTNGI MHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MEAIEHTTINTNGINMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           GFGDTDAPESHT YT  HIVGDLVGALD LGIEKVF+VGHDWGA++ WY CLFRPDR+KA
Sbjct: 61  GFGDTDAPESHTVYTPFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYLCLFRPDRVKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSV FL RNP ISFV+GFR A GDDFY+CRFQVPGEAEKDF SIDT  LFKKFLCGR
Sbjct: 121 LVNLSVHFLRRNPTISFVQGFRIAFGDDFYICRFQVPGEAEKDFGSIDTAELFKKFLCGR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
            P+P ILPKE GIRSIPSP+NLPSWL++EDIN+FATKF +TGFTGG NYYRALDL WELT
Sbjct: 181 SPDPPILPKEFGIRSIPSPQNLPSWLSQEDINYFATKFNRTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A W + QV VPVKF+VGDLDLTYH PGAKEY+HNGGFK DVPFLEEVVVMEDT HFINQE
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYHFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEIS HIHDFIKKF
Sbjct: 301 RPHEISAHIHDFIKKF 316

BLAST of Lag0031013 vs. ExPASy TrEMBL
Match: A0A1S3BXV2 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494764 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 268/316 (84.81%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TINTNGI MHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MEAIEHTTINTNGINMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           GFGDTDAPESHT YT  HIVGDLVGALD LGIEKVF+VGHDWGA++ WY CLFRPDR+KA
Sbjct: 61  GFGDTDAPESHTVYTPFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYLCLFRPDRVKA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VNLSV FL RNP ISFV+GFR A GDDFY+CRFQVPGEAEKDF SIDT  LFKKFLCGR
Sbjct: 121 LVNLSVHFLRRNPTISFVQGFRIAFGDDFYICRFQVPGEAEKDFGSIDTAELFKKFLCGR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
            P+P ILPKE GIRSIPSP+NLPSWL++EDIN+FATKF +TGFTGG NYYRALDL WELT
Sbjct: 181 SPDPPILPKEFGIRSIPSPQNLPSWLSQEDINYFATKFNRTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           A W + QV VPVKF+VGDLDLTYH PGAKEY+HNGGFK DVPFLEEVVVMEDT HFINQE
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYHFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           RPHEIS HIHDFIKKF
Sbjct: 301 RPHEISAHIHDFIKKF 316

BLAST of Lag0031013 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 9.9e-135
Identity = 203/316 (64.24%), Postives = 257/316 (81.33%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ IEH+TI+TNGI MH+ASIG+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GFGDTDAPESHTSYTMLHIVGDLVGALDVLGIEKVFLVGHDWGAVVGWYFCLFRPDRIKA 120
           G+GD+DAP S  SYT+LHIVGDLVG LD LG+++VFLVGHDWGA+V W+ C+ RPDR+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 FVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFLCGR 180
            VN SV F PRNP++  V+ FRA  GDD+Y+CRFQ PGE E+DFA +DT  L  +F   R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPNPLILPKEVGIRSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRALDLNWELT 240
           +P P  +PK VG R +P P +LP+WLTE+D+  +  KF Q GFTGGLNYYRAL+L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 APWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMEDTGHFINQE 300
           APWT +Q+ VPVKF+VGDLD+TY++PG KEY+H GG KK VPFL+EVVVME  GHF++QE
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 RPHEISTHIHDFIKKF 317
           +P E++ HI+ F KKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of Lag0031013 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 409.8 bits (1052), Expect = 1.9e-114
Identity = 183/323 (56.66%), Postives = 240/323 (74.30%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M+ I+H  ++ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGFGDTDAPESHTSYTMLHIVGDLVGALDVL--GIEKVFLVGHDWGAVVGWYFCLFRPD 120
           LRG+GD+D+PES + YT L++VGDLV  LD +    EKVFLVGHDWGA++GW+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKAFVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKF 180
           +I  FV LSV +  RNP +  V+GF+A  GDD+Y+CRFQ PG+ E + AS D  +  +  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LCGRDPNPLILPKE--VGIRSIPSPEN--LPSWLTEEDINHFATKFKQTGFTGGLNYYRA 240
             GR   P ILPK+   G +  P+ EN  LP W +++D++ + +KF++ GFTGGLNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 LDLNWELTAPWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMED 300
           +DLNWELTAPWT  ++ VPVKF+ GD D+ Y  PG KEY+H GGF  DVP L+E+VV+ED
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 TGHFINQERPHEISTHIHDFIKK 316
            GHF+NQE+P E++ HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of Lag0031013 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.3e-105
Identity = 169/324 (52.16%), Postives = 229/324 (70.68%), Query Frame = 0

Query: 1   MDVIEHSTINTNGIKMHIA-----SIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 60
           +D +EH T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGFGDTDAPESHTSYTMLHIVGDLVGALDVL--GIEKVFLVGHDWGAVVGWYFCLF 120
           APDLRG+GDT+APE    YT L++ GD+V  +D +  G + V +VGHDWGA++ W  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKAFVNLSVQFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLF 180
           RP+++KA VN+SV F PRNP    V   R   GDD+Y+CRFQ  GE E +F  + T  + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLCGRDPNPLILPKEVGI-RSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRA 240
           K+FL  + P PL LPK+    RS  +   LP WLT+ED++++ TK++  GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 LDLNWELTAPWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMED 300
           +D NWELTAPWT  ++ VPVKF++GD DLTY+ PGAKEY++ GGFK+DVP L+E VV++ 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 TGHFINQERPHEISTHIHDFIKKF 317
            GHF+++E P  I+ HIH+F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of Lag0031013 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 367.1 bits (941), Expect = 1.4e-101
Identity = 174/325 (53.54%), Postives = 225/325 (69.23%), Query Frame = 0

Query: 4   IEHSTINTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  +  NGI +H+A  G   GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  FGDTDAPESHTSYTMLHIVGDLVGALDVLGI---EKVFLVGHDWGAVVGWYFCLFRPDRI 123
           +GD+DAP   +SYT  +IVGDL+  +  L     EKVF+VGHDWGA++ WY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KAFVNLSV--QFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKF 183
           KA VNLSV   F P +P++  V+  RA  GDD+Y+CRFQ  G+ E + A + T  + K+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LCGRDPNPLILPKEVGI-----RSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYR 243
           L  R P P+I+PK+         +IP    LPSWLTEED+ +F +KF++ GF+G +NYYR
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFEEKGFSGPVNYYR 240

Query: 244 ALDLNWELTAPWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVME 303
             + N EL  PW   ++ VP KFV+G+LDL Y++PG KEY+H   FK+DVP LEE VVME
Sbjct: 241 NFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVME 300

Query: 304 DTGHFINQERPHEISTHIHDFIKKF 317
              HFINQE+P EI   I DFI KF
Sbjct: 301 GVAHFINQEKPQEILQIILDFISKF 321

BLAST of Lag0031013 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 5.1e-99
Identity = 170/324 (52.47%), Postives = 222/324 (68.52%), Query Frame = 0

Query: 4   IEHSTINTNGIKMHIASIG--TGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           +EH  +  NGI +H+A  G   G +VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  FGDTDAPESHTSYTMLHIVGDLVGALDVLGIE--KVFLVGHDWGAVVGWYFCLFRPDRIK 123
           +GD+DAP   +S+T  +IVGDLV  +  L  E  KVF+VGHDWGA++ WY CLFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 AFVNLSV--QFLPRNPAISFVEGFRAALGDDFYMCRFQVPGEAEKDFASIDTTLLFKKFL 183
           A VNLSV   F P +P++  V+  RA  G+D+Y+CRFQ  G+ E + A + T  + K+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 CGRDPNPLILPKEVGI-----RSIPSPENLPSWLTEEDINHFATKFKQTGFTGGLNYYRA 243
             R P PLI+PK+         +IP    LPSWLTEED+ +F +KFK+ GF G +NYYR 
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFKEKGFCGPVNYYRN 240

Query: 244 LDLNWELTAPWTRVQVGVPVKFVVGDLDLTYHLPGAKEYVHNGGFKKDVPFLEEVVVMED 303
            + N EL  PW   ++ VP KFV+G+LDL Y++PG KEY+H   FK+DVP +EE VVME 
Sbjct: 241 FNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEG 300

Query: 304 TGHFINQERPHEISTHIHDFIKKF 317
             HF+NQE+P EI   I DFI  F
Sbjct: 301 VAHFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934345.11.3e-16585.44uncharacterized protein LOC111441538 [Cucurbita moschata] >KAG6581272.1 hypothet... [more]
XP_023528763.13.0e-16585.76uncharacterized protein LOC111791601 [Cucurbita pepo subsp. pepo][more]
XP_022984060.11.5e-16485.44uncharacterized protein LOC111482484 [Cucurbita maxima][more]
XP_004152245.15.6e-16484.81uncharacterized protein LOC101205063 [Cucumis sativus] >KGN52816.1 hypothetical ... [more]
XP_008454330.19.5e-16484.81PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >TYK29511.1 bifu... [more]
Match NameE-valueIdentityDescription
I6YGS03.6e-5737.11Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
P349134.7e-4933.67Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
Q6Q2C21.1e-4833.22Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349143.4e-4733.78Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P802991.4e-4532.25Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1F7F36.4e-16685.44uncharacterized protein LOC111441538 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
A0A6J1J7M97.1e-16585.44uncharacterized protein LOC111482484 OS=Cucurbita maxima OX=3661 GN=LOC111482484... [more]
A0A0A0KY862.7e-16484.81AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A5D3E1N54.6e-16484.81Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BXV24.6e-16484.81bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494764 PE... [more]
Match NameE-valueIdentityDescription
AT4G02340.19.9e-13564.24alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.11.9e-11456.66alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.11.3e-10552.16alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.4e-10153.54soluble epoxide hydrolase [more]
AT2G26750.15.1e-9952.47alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 250..266
score: 37.43
coord: 290..312
score: 42.49
coord: 31..49
score: 55.26
coord: 97..110
score: 48.7
coord: 111..124
score: 44.81
coord: 51..66
score: 55.11
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 111..124
score: 22.17
coord: 51..66
score: 49.57
coord: 97..110
score: 28.08
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..163
e-value: 6.6E-21
score: 75.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..316
e-value: 5.7E-103
score: 347.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 4..315
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..316
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031013.1Lag0031013.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity