Lag0031002 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0031002
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like
Locationchr11: 3739450 .. 3744537 (-)
RNA-Seq ExpressionLag0031002
SyntenyLag0031002
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCAGGGTGGGGAATCACCGGTAACAAGGGCCGATGCTACGATTTCTGGATTGATTTCAGCGATTGTATGTCCCGCTGCCGAGAACCAAAGGACTGTGCTCTACTCCGTGAAGATTACCTCGAGTGCCTCCACCATTCCAAGGAGGTGAGACCTTTCATTTCACCTACTTTCTCGCTTCTGTTTTGTTTTTCCTTCAATTTTTCATGTATATGTTAGCTTAATTGCCTGAATAGGGATTAGATGTGTAGTTCCTTGATCCTTTGAGCTTTTGATGCCTAATTCGACCTTGCCTCATGTTTTCAACATCGTATCCGAAGGTGGCTGCCTGAGATTTGATCTAGATCTGTTTTTTGTATGTAACTTGTTCATTGAACAAATTTCTGGAGAAACTTGAATTTGCGACTACTTGGATTCTTGTGGTTTTAGATGTTAGTGATCGATTAGGGAAAGGTTGAGTCCTATCAATGAAAAGAAGAAGGTTTTGTGTCTTCAAGTTCCAGTTTCGCATGGTTTTTCAATATTGTGATGAATGTTTTAGAGTTGTCGTCATTAAGGATGAATGTTTTACAAAAGCACGGTGGAGCTCTCAGTTCAAGCCTCTTACTTAATTAGGTCGGGTTATCTATTTTTGCTTTAAGTGAACGGTTTGCCACACATTGCGTGTTATTGATTATCACGGCTGCCTCTTTGGTACTAGATATGGATTAGTGAAAGTTCTAGTCTGTTTGGGCATTTCTGTTAAGTCACATTAGCATGATTTTATGTTTCTGAAGATACATCCTTTTGGAACTGTGCATTCTGGCTCTAGAGAAATTATTCTATGAGGCTTAAACAATGTAACATACAGTTTTATAACCTTGGATGTGAGAAAGAGAAACAAAAAATTATGATTTAACCATGCATCTTAGGGTTTAGAGGGGACAGTTACAATGTAGTGGTAGTGGCCACATTGAATATTCTTTACCACTCTAACAAATGATCATTAAAATAAAACATCATACATTTACGTTCGTGTGGATACTACTTATCTAAGAAGATTTTTTTTTTTTTTGGAAAGATTATCTATGAAGATTTGAATTTGTGAAAATCCACTTAAACAACATCAAGCAAGAAAACTATGATAAGTTGATTTTTGGATGATGTGGCTGCTTTATGCATGCTTATTGAAACATGAAAGGAATAATAGAAAAATGTCTCTATGTGGGAGAAAAACAACTTTAATTACAAACAATGAGGAAATACAAAAGTAAGCTCTTATGTTGTTGGGGACATTCTATCTTGATGAGCATTTCACTATGGGTTGACCTAGTGGTCAATAAGGGCCCATGTAAATAATAAAGGCTTGAGAGGAAATGAGTTCAAGCCACGGTAGGTACCTACATAGGATTTAATATCCTATGAATTTCATTGGCAACCAAATATAGTAGAGTTAGGTGGTTGTTTCATGGGATTAGTCAAGATGTACGCAAGCTGATTTTGACACTCTGGATTTAAAAAAGAACTATCTCTATGAGCACTTTAATATTTACTTAACAGCATCAAGCTTTGCAACTATAGTCTTTCAATATTATTCCTCTTGGTGCTGAAATCCTCCACCTTCATCTCATTTTCAAATTTCATTTTCTGATATTCTGCCTATTTTCCACTGTTATTTCTTAGCTTTTGGGGGCACTTGGATCTCTTACTACTTCCTAATTTTCAACTTTTTTGTTGCATAATTGATAGATTCGTTCCTTTAATGTTGTCACTGGCACCATTAGCTTCGCATATTTGAGAATTTAAATAAACCTCTAGCCTACTTTTGTTAGTTTTTATGGCTTGGAAAAGTTACTCCCCTGTGCACATGAGTGGCCTTTTCCCCCCACTTTTGGTTTTGGTGTTGGTACCTGCTACTTGTCTAAAATTGTCTTCACTGTTAGTTCTTCTGAATGATTCTGCCTTGTGTATGATTTCAGGAAAGTACATGATAATATGAACAATTTTGTTCCGTGACTGTGAGAATAGCATTCACTTATCTTCCTTCATTCATGACATTTTCTATAAACTTGGTACTGATGATTTGTATTTGTAAACTGCTATTGCTCTATATTTCCATGCTTGGTATCAACCTCTTAACTTGTTTACTATTTCTTTTTTCTTTTTCTTCTTTTCCCCTTCTCAAGTGGTTTTTCTCTCTCAATTTTACTTTCATTTATCTTGGAGGTCGGTGGATTCCGATAAAAATTGTCTTTATTTTTTCGCTTAAGTCTAGCACCAAGTAACAAAAGCCACTGTGCTAGCTTTTTGTAGGAATAGGGTTATTTTTTTGAGCACGATCATATATTCACTATGCATTCAGATTAAAGAAAAAAACACCAGGCACAGGAGGAATAAAACATCCCAATATAAGGTGGCTCCAAAAATAGTAATTGGATTGAGGTTTATTTCTATCTCAAGAAAGCTATTTATCCCACTAAAATCAGAAAAGCTCATCTGAATGCATGGTGAATCTATGATCATGCTAAAAAAGTAGCACTAAAATCATTTCAATGTCAAGAAAACTATGTTCCTGTGCCTGTTGTGAGTTGTGTGATCTCTGCAACTGCTATCACGTACTATGTATAAGTTTATTATTTCACCTGTATGCACAGTTTTAGTGTTCATGCCGGAAAAGGTAGATAGGTGGATTTTTATTTTTATTTATTTATTTTTAAATTTTGCATCTATAAAATATAACCATCGGGTTGGTTCGTTAATCAAAAGTGCAAGCAGGTTACCCTAGATGCCTTTGAGGTCTCAGTTGTGGTTCAAAAATTAAATTATAGCCACCATCTACCTAAGATATTAAAAATATTTAACTAGTATTCAGACACACGATATATTGTAGGATCATGCGATTGTTCCATACAATTAGTTGGAAACTTGGGATGCATGTAAGGGAAAAAATCATTGAATCTGTAGAGAACCAAAATTTAAAAAGACTCCTATCGGGAGTGAGTTTAGCTGGTACTTGATTCTATCAATTACTAATTTGACCTTGATAACATAGCCACACATAAATTGCTTAGATTATGTTTGTTGTATTATTCTTGCAGTTGTTTATTTAACATAAAAATTAGTTGAATGAATTCATGGAACAGGAGAAAAACTGAACCCCTAAATTTTCACTTGTAAAAATTAAACCCTCAAACTTTAAAAATTGTAAAAATTGAACCCTTAAACTTTCAATCATAAAAATTAAACCCTCAAACTTTGATAAATATAGAAATTGGATCCATTTAATTATTTCATTTCTCCCTCATTTTTCATCTTTCTCTCTCCTCCTTCCACCCGTTCTCTTTCTGAAGAAGAAAGATTCACAAGAAAGTTATCCAAATAGACTTGTGAAAAAGGAAGAGAAAAAGCCCGTTCAAACCGCTGCCATTGAAGCCCTCGAAGAAGAAGAAGAAAGTGGACGTTTGTGGAACATCTATGGCGCCAGTGTTTTAAAAGGCGCGCCCGGGCGCGCGCCTAGGCGCAAGGCGCAACATTAGCGCCTCGCTTCGAAGATAGGCGCAAGGCGCAACATGACCGGGCAGCGAGACCCCCGTACTTGTCGTTCGGAGTGTCAAATATCATATTCAAGGCATATATTTTTTGTGGAGTATTGACATCATCATACTTGGTCGTTTGGAGTGTCAGAAGGCATATTAACAATACTAAACTCACTATTTTTTGTGGAGTATTGACATTTGACTCTATGTTGGAAGACTAATATTTGAACCTTATTTATCTTTTATCTATTTTTCTTAGTTAAAAATTCATTACTTGCCATGAAAGTGTTTTATATACATGTATATATTTTTGTAAATTTTTTTTCTATATGGTGCGTCTTACATAATAAAGCCCGCGCCTTTTCGTGCGCCTTGCGCCTAGGCTCCAGGAGGCCATTGCGCCTTAGTGCGCCTTGAGCCTTTTAAAACACTGTATGGCGCTTGTGTCTGTTATACGCAGAGGGAAAATGGGGATTGAAGAGAGTGAGAAAACACAAAACAGTAAACTAATAATGCAAGGGAAAGGGCAAAAACCACAGTGGAGTGATGATTCTGAAGCATTTTTGGATGGGTTAAATTAAATTAAATGGACACGAAGAAAGTTTCACGAAATGTAAATAATATTAAATTCTCCTCCTTCCACCCATTCTCTTTCTCTCCTCTTTTCATTTTGTGCCCGACTTTTGATCCACACAGAGTCCCAATTTCCATCCACTTCTTCTACCGTCGACTACACCCTTCAAGTTCCAACTTAGTTCTCGTCCTCAATCATTGGAACTGCAACTTTTTTCTTCAGAAAGAGAATGGGTGCAAAGAGGAGAGGAAGAGAGAATATGGAAGGAAGGAGGAGGGTTGAAAGAGGAGCGGAAGAGAGAATAGGGAAGGACGAGAGAGAAAAAGAGATGAAATATGAGGGAGAATTGAAGTAATTATATGGATCCAATTTTTATATATATGATAGTTTAAGGGTTTAATTTTTACAATTGAAAGTTTAGGGGTTCACTTTTTACAATTCTTAAAGTTTGAGGGTTTCATTTTTACACTTGAAAGTTTAGGGGTGTACTTGTTACAACCACTGTACTTCAAGGGTGGTTTTTGCAATTTGCCCTTTTATTTTTGTATAATGAGGTAAATATCTGGAATGAAACCCAGATTTCTAAGTTGTACCTATCATATTTGTTATGTACCTCTCTACTTGTATGATTATATTAGTGAGGAGTTTTATGGTGGCTCTGAAGAATTACTCTTTTTTTGGAGAGGTTAACAAAAAAGAAATTTAGTTGAAGTGTTTAATTACAGATATTCATTATATAAGGATTAAGTATGTCGTTGTTTAAGAATAGGAACACTTGGATAACCTTTCACAATGGAAGCCGAAGGAGTATGATCTACTTTTATTACAAATGGTTCTGCGTGATTTGTACCAATCTGTTCTAATTTAATTTTGCTTGTTTGAAATACCTTACAGTTCCAAAGAAGAAACCGTATTTATAAGGAGGAGCAGCGTAAACTAAGAGCAGCAGAACGGGAGGCCAAAGAGGGTAAACATGTTGATGCTGCTCATGCATAG

mRNA sequence

ATGGCATCAGGGTGGGGAATCACCGGTAACAAGGGCCGATGCTACGATTTCTGGATTGATTTCAGCGATTGTATGTCCCGCTGCCGAGAACCAAAGGACTGTGCTCTACTCCGTGAAGATTACCTCGAGTGCCTCCACCATTCCAAGGAGTTCCAAAGAAGAAACCGTATTTATAAGGAGGAGCAGCGTAAACTAAGAGCAGCAGAACGGGAGGCCAAAGAGGGTAAACATGTTGATGCTGCTCATGCATAG

Coding sequence (CDS)

ATGGCATCAGGGTGGGGAATCACCGGTAACAAGGGCCGATGCTACGATTTCTGGATTGATTTCAGCGATTGTATGTCCCGCTGCCGAGAACCAAAGGACTGTGCTCTACTCCGTGAAGATTACCTCGAGTGCCTCCACCATTCCAAGGAGTTCCAAAGAAGAAACCGTATTTATAAGGAGGAGCAGCGTAAACTAAGAGCAGCAGAACGGGAGGCCAAAGAGGGTAAACATGTTGATGCTGCTCATGCATAG

Protein sequence

MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKEEQRKLRAAEREAKEGKHVDAAHA
Homology
BLAST of Lag0031002 vs. NCBI nr
Match: XP_022155272.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Momordica charantia])

HSP 1 Score: 176.0 bits (445), Expect = 1.3e-40
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE
Sbjct: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60

Query: 61 EQRKLRAAEREAKEGKHVDAAHA 84
          EQRKLRAAEREAKEGKHVDAAHA
Sbjct: 61 EQRKLRAAEREAKEGKHVDAAHA 83

BLAST of Lag0031002 vs. NCBI nr
Match: XP_038905985.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B [Benincasa hispida])

HSP 1 Score: 174.9 bits (442), Expect = 3.0e-40
Identity = 82/83 (98.80%), Postives = 83/83 (100.00%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE
Sbjct: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60

Query: 61 EQRKLRAAEREAKEGKHVDAAHA 84
          EQRKLRAAER+AKEGKHVDAAHA
Sbjct: 61 EQRKLRAAERDAKEGKHVDAAHA 83

BLAST of Lag0031002 vs. NCBI nr
Match: XP_011652934.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B [Cucumis sativus] >KGN52808.1 hypothetical protein Csa_015424 [Cucumis sativus])

HSP 1 Score: 172.2 bits (435), Expect = 1.9e-39
Identity = 80/83 (96.39%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDC+LLREDYLECLHH KEFQRRNR+YKE
Sbjct: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCSLLREDYLECLHHFKEFQRRNRVYKE 60

Query: 61 EQRKLRAAEREAKEGKHVDAAHA 84
          EQRKLRAAEREAKEGKHVDAAHA
Sbjct: 61 EQRKLRAAEREAKEGKHVDAAHA 83

BLAST of Lag0031002 vs. NCBI nr
Match: XP_022948762.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Cucurbita moschata] >XP_022998534.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Cucurbita maxima] >KAG7036666.1 NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 171.4 bits (433), Expect = 3.3e-39
Identity = 80/83 (96.39%), Postives = 82/83 (98.80%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNR+YKE
Sbjct: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRVYKE 60

Query: 61 EQRKLRAAEREAKEGKHVDAAHA 84
          EQRKLRAA+ EAKEGKHVDAAHA
Sbjct: 61 EQRKLRAAKGEAKEGKHVDAAHA 83

BLAST of Lag0031002 vs. NCBI nr
Match: XP_023521877.1 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 169.9 bits (429), Expect = 9.5e-39
Identity = 79/83 (95.18%), Postives = 81/83 (97.59%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNR+YKE
Sbjct: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRVYKE 60

Query: 61 EQRKLRAAEREAKEGKHVDAAHA 84
          EQRKLR A+ EAKEGKHVDAAHA
Sbjct: 61 EQRKLRVAKGEAKEGKHVDAAHA 83

BLAST of Lag0031002 vs. ExPASy Swiss-Prot
Match: Q9LZI6 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B OS=Arabidopsis thaliana OX=3702 GN=At3g62790 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 2.5e-34
Identity = 66/74 (89.19%), Postives = 70/74 (94.59%), Query Frame = 0

Query: 1  MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
          MASGWGITGNKGRCYDFW+DFS+CMS CREPKDC LLREDYLECLHHSKEFQRRNRIYKE
Sbjct: 1  MASGWGITGNKGRCYDFWMDFSECMSHCREPKDCTLLREDYLECLHHSKEFQRRNRIYKE 60

Query: 61 EQRKLRAAEREAKE 75
          EQRKLRAA R+ +E
Sbjct: 61 EQRKLRAASRKGEE 74

BLAST of Lag0031002 vs. ExPASy Swiss-Prot
Match: O82238 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-A OS=Arabidopsis thaliana OX=3702 GN=At2g47690 PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 3.3e-34
Identity = 69/80 (86.25%), Postives = 72/80 (90.00%), Query Frame = 0

Query: 1   MASGWGITGNKGRCYDFWIDFSDCMSRCREPKDCALLREDYLECLHHSKEFQRRNRIYKE 60
           MASGWGITGNKGRCYDFW+DFS+CMS CREPKDC LLREDYLECLHHSKEFQRRNRIYKE
Sbjct: 36  MASGWGITGNKGRCYDFWMDFSECMSHCREPKDCTLLREDYLECLHHSKEFQRRNRIYKE 95

Query: 61  EQRKLRAAER---EAKEGKH 78
           EQRKLRAA R   EA +G H
Sbjct: 96  EQRKLRAASRKGEEAGDGTH 115

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155272.11.3e-40100.00NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Momordica charanti... [more]
XP_038905985.13.0e-4098.80NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B [Benincasa hispida][more]
XP_011652934.11.9e-3996.39NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B [Cucumis sativus] >KGN52... [more]
XP_022948762.13.3e-3996.39NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Cucurbita moschata... [more]
XP_023521877.19.5e-3995.18NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B-like [Cucurbita pepo sub... [more]
Match NameE-valueIdentityDescription
Q9LZI62.5e-3489.19NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-B OS=Arabidopsis thaliana ... [more]
O822383.3e-3486.25NADH dehydrogenase [ubiquinone] iron-sulfur protein 5-A OS=Arabidopsis thaliana ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 51..78
NoneNo IPR availablePANTHERPTHR15224:SF5BNAC04G00180D PROTEINcoord: 1..82
NoneNo IPR availablePROSITEPS51808CHCHcoord: 11..52
score: 11.175484
IPR019342NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5PFAMPF10200Ndufs5coord: 12..65
e-value: 1.8E-6
score: 28.1
IPR019342NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5PANTHERPTHR15224NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5coord: 1..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0031002.1Lag0031002.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
cellular_component GO:0005747 mitochondrial respiratory chain complex I