Lag0030148 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0030148
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPI-PLC X domain-containing protein
Locationchr8: 44919537 .. 44922379 (+)
RNA-Seq ExpressionLag0030148
SyntenyLag0030148
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAATCCCATTTGCAGAAGAAGAAACTTGTAATACTCCTAATTTCATTGCTCATCTTCTTCTCACTACTCATTGTTGAAGCTGAAGAAACCTGCTCCAATGGACTCTGCAAGGTTGTTTCTTTTCTTTTTTTCTCTCTGCTATGGATTAATGAATGATGACATTTTTTTTATCTCTATATTAATTGTTGTTTTGTTTTTTTAAGGTGGGGGATGAGTGCTCTTCAAGTGGAGATTGTGGAGGTGGATTGTATTGTTTTTCATGCACACCAACAAGCTTCTTTGCCTCTAAATGTGTTAGATCTCTTTACACTGATCAGTTCAAACTTCTGGTGTGCAATTTTCTCCTTTTTTTTTCTTTTTTTTGTGCATCATATTGTGTTTGATTGTTTGTGTGTCTGGGATTTTCAACCTTTGGTTTTTTTTCTTTTTTCTCTTTGGTACCTATGGTTGTTAAAACACAATAAAACGCTTTCCCTCCGTGAGTTTAGATCCTCGTTCGATAACCATTTAGTTTTTGGATTTTTTGCTTTTGAAACTTGTGTTTTAGAACTGACCAAAAATGTTAGGTGGGGTTGGATTAGTGTGAGACCTAAGACATTGAATGGGGTGGAAAAAATTCCTATATTCCAAATGGAATTTCGAGTCAAAATAAATATGAAAATAAATATTTTCTATTGTGGGAAAACTTGGAAAGTCTCCAACCCCCAACTTTGATTCTTTCGACGTGGGAGATGGGCACAAGACATCTCTCTAACTCTCCTTAATTTAATTGTAGAACTTTTCTTTTTTCTTTTCTTAGAAGGGATTTTCATTTCCCTTACTATAAAAAAGATTGTGAAAGAAAAAGATGACAAATAATGAAAGGGAAAAAATCTTACAAAAAAATTGCAAAAACTATACTATAAATTAAAACAAATGTAACTGTCATATACCACATAGAATTAATGTAACTCCAATGTTATATTTAACATAAAATTAGGTACACAGAACAAGAAAACAAGACTATTGCAGCAGATAATAACCATTGCAACACTTTTGGAAGTGGTTTATGAACCCTTGGCAGAAGTGGTGTTAATAATTAAGAAGGATCTAGCAACGACATTTCTAAAAAATTTTAGAACGACTTCTTCTCACAAATGCTACGACTTCTTCAAAGATATATAAATCTAAAGTGTATATACACACACGCATACTCTCTTTCTCTCCGTTATTTTTTCATAAACAATAACTTTAACAGTTAGACTTACATTTAAACATTATTATAAGGAATATTGATGATAATAAGTGCCAAAGTTTGTATGAATATTTTTGTAAAATTGTATAATAATATATATCTGTTTTGATGCAGAACAATTCATTGGCATTCAACAAGTATGCATATCTAACAACTCACAATTCATATGCTATTGAAGGAGAGCCTTCTCACACAGGACTCCCACGTGTTACCCTTAACAATCAAGAGGACACAATTTCCCAACAACTTAATGTACACTATATATATATAATAACTTAATTATTATTAGGCCAAAATTATATTTTATTTTACCCTTTAATTAATGTAATAATTCTGATGTTAATTATTTTCTAAACCCCATTTTTGCAGAATGGAGTTAGAGCTTTTATGCTGGATACCTATGATTTTCTTGGAGATGTTTGGCTCTGCCATTCCTTTGGAGGAAAATGCTACAACTACACTGCATTTGTATGTTAATATATATTCTTATGATCTTTACTACATTTTTCGGGGTTAAACTTAATTTACCCTTGTTAATTTTGTGATGCGATTCACGCGAATTATTCTATTTTGACACACTTGACTTGAAAAATCTAAAAGTATAAAGGGTTTGTTGTGCGAGTGGATTTTCGAAATAGTGTAGTTTGGATATTCTTCTATGTCTTTGTAGTTTTCAACTAAATTTACCCTAATTGCAATATTTGAATTGCAATTAGGGTTAGGGTTTTGTAAATTTACCCTAATTCTATTTTTAACTCCACTTGAAAATTTGTTATAGGAACCAGCACTAGACACATTGAAGGTAATAGAAGCCTTTTTAGCAGCAAACACACAAGAGATTGTCACATTGATCTTAGAAGACTATGTTCAATCTCCCAATGGATTGAGCAAGGTGTTCACCGACGCCGGGCTGAAGAAGTTCTGGTTTCCGATCACGAACATGCCGAAGAACGGTAACGACTGGCCCCGAGTCAGCGACATGGTGGCGAACAACCAAAGGCTGCTGGTCTTCACATCAAATAGAACAAAGGAAGCAACAGAAGGAATAGCATACCAATGGAATTACATGGTTGAAAATCAGTGTAAGAAAAAATACCCCTGAACTATGGGGTTTGTTTAAAAAATACCCCTGAAGTTTGAAAAATGTTTCAAAAATACGGTTGCAGATGGGAATGATGGAATGCAAGAAGGGAGTTGTCCAAAGAGGGCAGAATCATCAGCACTTGATGACAAGGGAAAATCACTGATTTTGGTTAACTATTTTCCAACAGTTCCATTCAAGAAACAAGCATGTGAGCATAATTCTAAGGATCTTTCTGCCATGCTCCAAACTTGTCATCAGGCAGCTGCTAACAGATGGGCTAACTTTGTGGCTCTTGATTTCTATAAGGTTAATTTTCTCTTCAAAATAACTTTCAAATATATTGAGATCAAATTTTAATTTTTTCTTCTTTTAAAACTAAGTAAAAAAATTCAATTTATATATATATATATATATATATATATGCAGAGGAGTGATGGAGGAGGGGCATTTCAAGCTTTGGATACTTTGAATGGGGAGCTCTTGTGTGCATCAAAGGACATTCATTCATGTGTGGGGTAA

mRNA sequence

ATGGACAAATCCCATTTGCAGAAGAAGAAACTTGTAATACTCCTAATTTCATTGCTCATCTTCTTCTCACTACTCATTGTTGAAGCTGAAGAAACCTGCTCCAATGGACTCTGCAAGGTGGGGGATGAGTGCTCTTCAAGTGGAGATTGTGGAGGTGGATTGTATTGTTTTTCATGCACACCAACAAGCTTCTTTGCCTCTAAATGTGTTAGATCTCTTTACACTGATCAGTTCAAACTTCTGAACAATTCATTGGCATTCAACAAGTATGCATATCTAACAACTCACAATTCATATGCTATTGAAGGAGAGCCTTCTCACACAGGACTCCCACGTGTTACCCTTAACAATCAAGAGGACACAATTTCCCAACAACTTAATAATGGAGTTAGAGCTTTTATGCTGGATACCTATGATTTTCTTGGAGATGTTTGGCTCTGCCATTCCTTTGGAGGAAAATGCTACAACTACACTGCATTTGAACCAGCACTAGACACATTGAAGGTAATAGAAGCCTTTTTAGCAGCAAACACACAAGAGATTGTCACATTGATCTTAGAAGACTATGTTCAATCTCCCAATGGATTGAGCAAGGTGTTCACCGACGCCGGGCTGAAGAAGTTCTGGTTTCCGATCACGAACATGCCGAAGAACGGTAACGACTGGCCCCGAGTCAGCGACATGGTGGCGAACAACCAAAGGCTGCTGGTCTTCACATCAAATAGAACAAAGGAAGCAACAGAAGGAATAGCATACCAATGGAATTACATGGTTGAAAATCAGTATGGGAATGATGGAATGCAAGAAGGGAGTTGTCCAAAGAGGGCAGAATCATCAGCACTTGATGACAAGGGAAAATCACTGATTTTGGTTAACTATTTTCCAACAGTTCCATTCAAGAAACAAGCATGTGAGCATAATTCTAAGGATCTTTCTGCCATGCTCCAAACTTGTCATCAGGCAGCTGCTAACAGATGGGCTAACTTTGTGGCTCTTGATTTCTATAAGAGGAGTGATGGAGGAGGGGCATTTCAAGCTTTGGATACTTTGAATGGGGAGCTCTTGTGTGCATCAAAGGACATTCATTCATGTGTGGGGTAA

Coding sequence (CDS)

ATGGACAAATCCCATTTGCAGAAGAAGAAACTTGTAATACTCCTAATTTCATTGCTCATCTTCTTCTCACTACTCATTGTTGAAGCTGAAGAAACCTGCTCCAATGGACTCTGCAAGGTGGGGGATGAGTGCTCTTCAAGTGGAGATTGTGGAGGTGGATTGTATTGTTTTTCATGCACACCAACAAGCTTCTTTGCCTCTAAATGTGTTAGATCTCTTTACACTGATCAGTTCAAACTTCTGAACAATTCATTGGCATTCAACAAGTATGCATATCTAACAACTCACAATTCATATGCTATTGAAGGAGAGCCTTCTCACACAGGACTCCCACGTGTTACCCTTAACAATCAAGAGGACACAATTTCCCAACAACTTAATAATGGAGTTAGAGCTTTTATGCTGGATACCTATGATTTTCTTGGAGATGTTTGGCTCTGCCATTCCTTTGGAGGAAAATGCTACAACTACACTGCATTTGAACCAGCACTAGACACATTGAAGGTAATAGAAGCCTTTTTAGCAGCAAACACACAAGAGATTGTCACATTGATCTTAGAAGACTATGTTCAATCTCCCAATGGATTGAGCAAGGTGTTCACCGACGCCGGGCTGAAGAAGTTCTGGTTTCCGATCACGAACATGCCGAAGAACGGTAACGACTGGCCCCGAGTCAGCGACATGGTGGCGAACAACCAAAGGCTGCTGGTCTTCACATCAAATAGAACAAAGGAAGCAACAGAAGGAATAGCATACCAATGGAATTACATGGTTGAAAATCAGTATGGGAATGATGGAATGCAAGAAGGGAGTTGTCCAAAGAGGGCAGAATCATCAGCACTTGATGACAAGGGAAAATCACTGATTTTGGTTAACTATTTTCCAACAGTTCCATTCAAGAAACAAGCATGTGAGCATAATTCTAAGGATCTTTCTGCCATGCTCCAAACTTGTCATCAGGCAGCTGCTAACAGATGGGCTAACTTTGTGGCTCTTGATTTCTATAAGAGGAGTGATGGAGGAGGGGCATTTCAAGCTTTGGATACTTTGAATGGGGAGCTCTTGTGTGCATCAAAGGACATTCATTCATGTGTGGGGTAA

Protein sequence

MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSCVG
Homology
BLAST of Lag0030148 vs. NCBI nr
Match: XP_023549235.1 (PI-PLC X domain-containing protein At5g67130 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.4 bits (1687), Expect = 5.6e-184
Identity = 317/366 (86.61%), Postives = 336/366 (91.80%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     +LL+S LIF    +V A++TCS+GLCKVGD CSS+GDCG GLYCFSC 
Sbjct: 1   MEISHWQSSLHSVLLLSFLIF---PLVAADQTCSDGLCKVGDGCSSNGDCGPGLYCFSCP 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYTD FKLLNNSL FNKYAYLTTHNS+AIEGEPSHTG+PRVTLNNQED
Sbjct: 61  PT-FFASRCVRSLYTDPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSHTGVPRVTLNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQLNNGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 121 TIAQQLNNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFTDAGLKKFWFPITNMPK+ +DWP VSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTDAGLKKFWFPITNMPKDNDDWPLVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 241 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNSKDL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 301 RQACEHNSKDLIGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. NCBI nr
Match: XP_022953911.1 (PI-PLC X domain-containing protein At5g67130 [Cucurbita moschata])

HSP 1 Score: 649.8 bits (1675), Expect = 1.4e-182
Identity = 315/366 (86.07%), Postives = 335/366 (91.53%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    +V A++TCSNGLCK+GD CSS+GDCG GLYC SC 
Sbjct: 43  MEISHWQSSLHPILLLSFLIF---PLVAADQTCSNGLCKLGDGCSSNGDCGPGLYCLSCP 102

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYTD FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 103 PT-FFASRCVRSLYTDPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 162

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQLNNGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 163 TIAQQLNNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 222

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFT+AGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 223 IVTLILEDYVQSPNGLTKVFTEAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 282

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 283 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 342

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 343 RQACEHNSEDLVGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 402

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 403 IHSCVG 404

BLAST of Lag0030148 vs. NCBI nr
Match: XP_022990547.1 (PI-PLC X domain-containing protein At5g67130 [Cucurbita maxima])

HSP 1 Score: 648.7 bits (1672), Expect = 3.1e-182
Identity = 315/366 (86.07%), Postives = 334/366 (91.26%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    +V A++TCSNGLCKVGD CSS+GDCG GLYC SC 
Sbjct: 1   MEISHWQSSLHSILLLSFLIF---PLVAADQTCSNGLCKVGDGCSSNGDCGAGLYCLSCP 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYT  FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 61  PT-FFASRCVRSLYTHPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQL+NGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 121 TIAQQLDNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFTDAGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTDAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 241 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 301 RQACEHNSEDLVGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. NCBI nr
Match: KAG7033214.1 (PI-PLC X domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648.7 bits (1672), Expect = 3.1e-182
Identity = 314/366 (85.79%), Postives = 335/366 (91.53%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    ++ A++TCSNGLCK+GD CSS+GDCG GLYC SC 
Sbjct: 1   MEISHWQSSLHPILLLSFLIF---PLLAADQTCSNGLCKLGDGCSSNGDCGPGLYCLSCP 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYTD FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 61  PT-FFASRCVRSLYTDPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQLNNGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 121 TIAQQLNNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFT+AGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTEAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 241 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 301 RQACEHNSEDLVGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. NCBI nr
Match: KAG6602537.1 (PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-182
Identity = 314/366 (85.79%), Postives = 334/366 (91.26%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    +V A++TCSNGLCK+GD CSS+GDCG GLYC SC 
Sbjct: 1   MEISHWQSSLHPILLLSFLIF---PLVAADQTCSNGLCKLGDGCSSNGDCGPGLYCLSCP 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYTD FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 61  PT-FFASRCVRSLYTDPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQLNNGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 121 TIAQQLNNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFT+AGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTEAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 241 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH  A NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 301 RQACEHNSEDLIGMLQTCHGEAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. ExPASy Swiss-Prot
Match: Q93XX5 (PI-PLC X domain-containing protein At5g67130 OS=Arabidopsis thaliana OX=3702 GN=At5g67130 PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 3.9e-103
Identity = 177/356 (49.72%), Postives = 236/356 (66.29%), Query Frame = 0

Query: 9   KKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASK 68
           + + + L+ LL+ FS         CSNG C++ D CSS+ DC  GLYC  C         
Sbjct: 10  RAVTVSLLLLLLSFSF-----SSACSNGNCQLLDSCSSATDCVSGLYCGDCPAVGRSKPV 69

Query: 69  CVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNN 128
           C R   T    ++ N L FNKY +L THN+++    P   G+ R+T  NQEDTI+ QL N
Sbjct: 70  CTRGQATSPTSII-NGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQN 129

Query: 129 GVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILED 188
           GVR  MLD YDF  D+WLCHS  G+C+N+TAF+PA++ L+ +EAFL+ N  EIVT+I+ED
Sbjct: 130 GVRGLMLDMYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIED 189

Query: 189 YVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATE 248
           YV  P GLS +F +AGL K+WFP++ MP+ G DWP V+DMV  N RLLVFTS   KE  E
Sbjct: 190 YVHRPKGLSTLFANAGLDKYWFPVSKMPRKGEDWPTVTDMVQENHRLLVFTSVAAKEDEE 249

Query: 249 GIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNS 308
           G+AYQW YMVEN+ G+ G++ GSCP R ES  L+ K  SL L+NYFPT P +K AC+ +S
Sbjct: 250 GVAYQWRYMVENESGDPGVKRGSCPNRKESQPLNSKSSSLFLMNYFPTYPVEKDACKEHS 309

Query: 309 KDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
             L+ M+ TC ++  NR  NF+A++FY RSDGGG F+ LD +NG +LC  + + +C
Sbjct: 310 APLAEMVGTCLKSGGNRMPNFLAVNFYMRSDGGGVFEILDRMNGPVLCGCETLSAC 359

BLAST of Lag0030148 vs. ExPASy Swiss-Prot
Match: D4AUX6 (Uncharacterized secreted protein ARB_08043 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_08043 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.2e-06
Identity = 63/261 (24.14%), Postives = 108/261 (41.38%), Query Frame = 0

Query: 116 NNQEDTISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLA 175
           +NQE  +  QLN+G+R     T+   G ++LCHS      N    E     LK +  +L 
Sbjct: 149 SNQELDVVTQLNDGIRMLQFQTHLVNGTIYLCHS-SCDLLNAGTLE---SYLKKVADWLR 208

Query: 176 ANTQEIVTLIL--EDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQ 235
            N  ++V+L++   D+V   N  + +   +GL    +   N     +DWP +S+++ + +
Sbjct: 209 DNPYDVVSLLIGNGDFVGVKNFTAPI-QSSGLIDHVYTPKNHSIALDDWPTLSEVILSGK 268

Query: 236 RLLVFTSNRTKEATEG----IAYQWNYMVENQYG------------NDGMQEGSCPKRAE 295
           R +VF      EA  G    I  ++ Y+ E  +               G+ E    KR  
Sbjct: 269 RAMVFMD---YEANHGEVPYILDEFTYIWETPFSPTDRNFPCDIQRPPGLNEADARKRMY 328

Query: 296 SS------ALDDKGKSLILVNYFPTVPFKKQACEHNSKDLSAMLQTCHQAAANRWANFVA 352
            +       +   G ++++ N   TV   +         + AM   C +   NR  NF+ 
Sbjct: 329 MANHNLNLEISIAGATILVPN---TVLLNETNAVSGFGSMGAMAGNCTE-KWNRPPNFLL 388

BLAST of Lag0030148 vs. ExPASy TrEMBL
Match: A0A6J1GPE3 (PI-PLC X domain-containing protein At5g67130 OS=Cucurbita moschata OX=3662 GN=LOC111456311 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.7e-183
Identity = 315/366 (86.07%), Postives = 335/366 (91.53%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    +V A++TCSNGLCK+GD CSS+GDCG GLYC SC 
Sbjct: 43  MEISHWQSSLHPILLLSFLIF---PLVAADQTCSNGLCKLGDGCSSNGDCGPGLYCLSCP 102

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYTD FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 103 PT-FFASRCVRSLYTDPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 162

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQLNNGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 163 TIAQQLNNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 222

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFT+AGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 223 IVTLILEDYVQSPNGLTKVFTEAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 282

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 283 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 342

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 343 RQACEHNSEDLVGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 402

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 403 IHSCVG 404

BLAST of Lag0030148 vs. ExPASy TrEMBL
Match: A0A6J1JJ27 (PI-PLC X domain-containing protein At5g67130 OS=Cucurbita maxima OX=3661 GN=LOC111487395 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.5e-182
Identity = 315/366 (86.07%), Postives = 334/366 (91.26%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+ SH Q     ILL+S LIF    +V A++TCSNGLCKVGD CSS+GDCG GLYC SC 
Sbjct: 1   MEISHWQSSLHSILLLSFLIF---PLVAADQTCSNGLCKVGDGCSSNGDCGAGLYCLSCP 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PT FFAS+CVRSLYT  FKLLNNSL FNKYAYLTTHNS+AIEGEPS TG+PRVTLNNQED
Sbjct: 61  PT-FFASRCVRSLYTHPFKLLNNSLPFNKYAYLTTHNSFAIEGEPSRTGVPRVTLNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           TI+QQL+NGVR FMLDTYDFLGDVWLCHSFGGKC+NYTAFEPALDTLKVIEAFLAANT+E
Sbjct: 121 TIAQQLDNGVRGFMLDTYDFLGDVWLCHSFGGKCHNYTAFEPALDTLKVIEAFLAANTKE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFTDAGLKKFWFPITNMPK+ +DWPRVSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTDAGLKKFWFPITNMPKDNDDWPRVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NR KEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDK KSL+LVNYFPTVPFK
Sbjct: 241 NRAKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKRKSLVLVNYFPTVPFK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           +QACEHNS+DL  MLQTCH AA NRWANFVALDFYKRSDGGGAFQALDTLNGELLC S D
Sbjct: 301 RQACEHNSEDLVGMLQTCHGAAGNRWANFVALDFYKRSDGGGAFQALDTLNGELLCGSTD 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. ExPASy TrEMBL
Match: A0A6J1C0L7 (PI-PLC X domain-containing protein At5g67130 OS=Momordica charantia OX=3673 GN=LOC111006360 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 6.1e-176
Identity = 301/366 (82.24%), Postives = 326/366 (89.07%), Query Frame = 0

Query: 1   MDKSHLQKKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCT 60
           M+KSH   K   +++I LL+   L  V A+E C NGLCKVG+ECSSSGDCG GLYC SC 
Sbjct: 1   MEKSHHLHK---LVIIQLLVSIFLFSVAAQEPCPNGLCKVGEECSSSGDCGDGLYCSSC- 60

Query: 61  PTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQED 120
           PTSFF S+CVRS YT+QFKL+NNSL FNKYAYLTTHNS+AIEGEPSH G+PRVT NNQED
Sbjct: 61  PTSFFGSRCVRSTYTNQFKLVNNSLPFNKYAYLTTHNSFAIEGEPSHIGIPRVTFNNQED 120

Query: 121 TISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQE 180
           T+SQQLNNGVRA MLDTYDF GDVWLCHSFGGKC+NYTAFEPA+DTLK +EAFL ANT E
Sbjct: 121 TVSQQLNNGVRALMLDTYDFDGDVWLCHSFGGKCHNYTAFEPAIDTLKEVEAFLTANTAE 180

Query: 181 IVTLILEDYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTS 240
           IVTLILEDYVQSPNGL+KVFTDAGLKKFWFPITNMPKNG+DWPRVSDMVANNQRLLVFTS
Sbjct: 181 IVTLILEDYVQSPNGLTKVFTDAGLKKFWFPITNMPKNGSDWPRVSDMVANNQRLLVFTS 240

Query: 241 NRTKEATEGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFK 300
           NRTKEATEGIAYQWNYMVENQYGNDGM+EGSC  R ESSALDDKGKSLIL+NYFPTVP K
Sbjct: 241 NRTKEATEGIAYQWNYMVENQYGNDGMKEGSCANRGESSALDDKGKSLILMNYFPTVPIK 300

Query: 301 KQACEHNSKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKD 360
           + AC+HNSKDL AML TCHQAAANRW NF+A+DFYKRSDGGGAFQALD  NG LLC S+D
Sbjct: 301 QLACQHNSKDLFAMLLTCHQAAANRWPNFLAVDFYKRSDGGGAFQALDASNGGLLCNSED 360

Query: 361 IHSCVG 367
           IHSCVG
Sbjct: 361 IHSCVG 362

BLAST of Lag0030148 vs. ExPASy TrEMBL
Match: A0A5A7TBL4 (PI-PLC X domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002330 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.0e-175
Identity = 303/356 (85.11%), Postives = 320/356 (89.89%), Query Frame = 0

Query: 14  LLISLLIFFSLLI---VEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASKCV 73
           LLISL + FSLL+   V A  TCSNG CKVGDECSSS DCG GLYCFSC PT F  SKCV
Sbjct: 13  LLISLFLIFSLLLEAAVAAAITCSNGPCKVGDECSSSEDCGDGLYCFSCIPTFFGGSKCV 72

Query: 74  RSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNNGV 133
           RS YT+QFKLLNNSL FNKYAYL+THNS+AIEGEPS TG+PRVTLNNQEDTISQQLNNGV
Sbjct: 73  RSTYTNQFKLLNNSLPFNKYAYLSTHNSFAIEGEPSQTGVPRVTLNNQEDTISQQLNNGV 132

Query: 134 RAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILEDYV 193
           RAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPAL+TLK IEAFL ANT+EIVTLILEDYV
Sbjct: 133 RAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALNTLKEIEAFLEANTEEIVTLILEDYV 192

Query: 194 QSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATEGI 253
           QSPNGL+KVFT+AGLKKFWFPITNMPKNG DWPRVSDMVANNQRLLVFTSN TKEA EGI
Sbjct: 193 QSPNGLTKVFTNAGLKKFWFPITNMPKNGEDWPRVSDMVANNQRLLVFTSNNTKEAAEGI 252

Query: 254 AYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNSKD 313
           AYQWNYMVENQYGNDGM+EGSC  R ESS LDDKGKSLIL+NYFPTVP K +AC  NSK+
Sbjct: 253 AYQWNYMVENQYGNDGMKEGSCSNRGESSRLDDKGKSLILMNYFPTVPLKIEACVDNSKN 312

Query: 314 LSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSCVG 367
           L  MLQTCH +A NRW NFVA+DFYKRSDGGGAFQALDTLNGELLCAS+DIHSCVG
Sbjct: 313 LLGMLQTCHGSAGNRWPNFVAVDFYKRSDGGGAFQALDTLNGELLCASQDIHSCVG 368

BLAST of Lag0030148 vs. ExPASy TrEMBL
Match: A0A1S3C9K6 (PI-PLC X domain-containing protein At5g67130 OS=Cucumis melo OX=3656 GN=LOC103498544 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.7e-175
Identity = 302/355 (85.07%), Postives = 319/355 (89.86%), Query Frame = 0

Query: 14  LLISLLIFFSLLI--VEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASKCVR 73
           LLISL + FSLL+    A  TCSNG CKVGDECSSS DCG GLYCFSC PT F  SKCVR
Sbjct: 13  LLISLFLIFSLLLEAAVAAATCSNGPCKVGDECSSSEDCGDGLYCFSCIPTFFGGSKCVR 72

Query: 74  SLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNNGVR 133
           S YT+QFKLLNNSL FNKYAYL+THNS+AIEGEPS TG+PRVTLNNQEDTISQQLNNGVR
Sbjct: 73  STYTNQFKLLNNSLPFNKYAYLSTHNSFAIEGEPSQTGVPRVTLNNQEDTISQQLNNGVR 132

Query: 134 AFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILEDYVQ 193
           AFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPAL+TLK IEAFL ANT+EIVTLILEDYVQ
Sbjct: 133 AFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALNTLKEIEAFLEANTEEIVTLILEDYVQ 192

Query: 194 SPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATEGIA 253
           SPNGL+KVFT+AGLKKFWFPITNMPKNG DWPRVSDMVANNQRLLVFTSN TKEA EGIA
Sbjct: 193 SPNGLTKVFTNAGLKKFWFPITNMPKNGEDWPRVSDMVANNQRLLVFTSNNTKEAAEGIA 252

Query: 254 YQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNSKDL 313
           YQWNYMVENQYGNDGM+EGSC  R ESS LDDKGKSLIL+NYFPTVP K +AC  NSK+L
Sbjct: 253 YQWNYMVENQYGNDGMKEGSCSNRGESSRLDDKGKSLILMNYFPTVPLKIEACVDNSKNL 312

Query: 314 SAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSCVG 367
             MLQTCH AA NRW NFVA+DFYKRSDGGGAFQALDTLNG+LLCAS+DIHSCVG
Sbjct: 313 LGMLQTCHGAAGNRWPNFVAVDFYKRSDGGGAFQALDTLNGKLLCASQDIHSCVG 367

BLAST of Lag0030148 vs. TAIR 10
Match: AT1G13680.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 431.0 bits (1107), Expect = 9.4e-121
Identity = 206/331 (62.24%), Postives = 248/331 (74.92%), Query Frame = 0

Query: 34  SNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASKCVRSLYTDQFKLLNNSLAFNKYAYL 93
           S G  ++GD+CSS  DC  GL CF C       ++CVRS  TDQF ++NNS+ FNKYA+L
Sbjct: 26  SYGSLQLGDQCSSDEDCNVGLGCFKC---GIDVARCVRSNITDQFSIVNNSMPFNKYAFL 85

Query: 94  TTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNNGVRAFMLDTYDFLGDVWLCHSFGGK 153
           TTHNSYAIEG+  H          QEDTI QQLN+GVRA MLDTYD+ GDVW CHSF  +
Sbjct: 86  TTHNSYAIEGKALHVA-------TQEDTIVQQLNSGVRALMLDTYDYEGDVWFCHSFDEQ 145

Query: 154 CYNYTAFEPALDTLKVIEAFLAANTQEIVTLILEDYVQSPNGLSKVFTDAGLKKFWFPIT 213
           C+ +T F  A+DT K I AFL AN  EIVTLILEDYV+S NGL+KVFTD+GLKKFWFP+ 
Sbjct: 146 CFEFTKFNRAIDTFKEIFAFLTANPSEIVTLILEDYVKSQNGLTKVFTDSGLKKFWFPVQ 205

Query: 214 NMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATEGIAYQWNYMVENQYGNDGMQEGSCP 273
           NMP  G DWP V DMVANN RL+VFTS ++K+ TEGIAYQWNYMVENQYG+DG++   C 
Sbjct: 206 NMPIGGQDWPLVKDMVANNHRLIVFTSAKSKQETEGIAYQWNYMVENQYGDDGVKPDECS 265

Query: 274 KRAESSALDDKGKSLILVNYFPTVPFKKQACEHNSKDLSAMLQTCHQAAANRWANFVALD 333
            RA+S+ L DK K+L+ VN+F TVP K   CE NS+ L  M++TC+ AA NRWANFVA++
Sbjct: 266 NRADSALLTDKTKALVSVNHFKTVPVKILTCEENSEQLLDMIKTCYVAAGNRWANFVAVN 325

Query: 334 FYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
           FYKRS+GGG FQA+D LNGELLC   D+H+C
Sbjct: 326 FYKRSNGGGTFQAIDKLNGELLCGRDDVHAC 346

BLAST of Lag0030148 vs. TAIR 10
Match: AT1G49740.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.5e-107
Identity = 189/357 (52.94%), Postives = 248/357 (69.47%), Query Frame = 0

Query: 8   KKKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFAS 67
           ++ L+ LLI+LL+  S L+       S+ L K G  C ++ +C  GL+C +C   + F  
Sbjct: 3   QRLLLFLLIALLLQSSFLL-----EISSAL-KEGKTCITNSNCDAGLHCETCIANTDFRP 62

Query: 68  KCVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLN 127
           +C R+   +        L FNKY++LTTHNS+A  GE S TG   +   NQ+D+I+ QLN
Sbjct: 63  RCSRTQPINPITKA-KGLPFNKYSWLTTHNSFARLGEVSRTGSAILAPTNQQDSITSQLN 122

Query: 128 NGVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILE 187
           NGVR FMLD YDF  D+WLCHSF G C+N+TAF+PA++ L+  + FL  N +E+VT+I+E
Sbjct: 123 NGVRGFMLDMYDFQNDIWLCHSFDGTCFNFTAFQPAINILREFQVFLEKNKEEVVTIIIE 182

Query: 188 DYVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEAT 247
           DYV+SP GL+KVF  AGL+KF FP++ MPKNG DWPR+ DMV  NQRLLVFTS+  KEAT
Sbjct: 183 DYVKSPKGLTKVFDAAGLRKFMFPVSRMPKNGGDWPRLDDMVRKNQRLLVFTSDSHKEAT 242

Query: 248 EGIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHN 307
           EGIAYQW YMVENQYGN G++ G CP RA+S+ + DK KSL+LVN+FP       AC+ N
Sbjct: 243 EGIAYQWKYMVENQYGNGGLKVGVCPNRAQSAPMSDKSKSLVLVNHFPDAADVIVACKQN 302

Query: 308 SKDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
           S  L   ++TC+QAA  RW NF+A+DFYKRSDGGGA QA+D  NG L+C   +  +C
Sbjct: 303 SASLLESIKTCYQAAGQRWPNFIAVDFYKRSDGGGAPQAVDVANGNLICGCDNFAAC 352

BLAST of Lag0030148 vs. TAIR 10
Match: AT4G36945.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.0e-106
Identity = 187/344 (54.36%), Postives = 235/344 (68.31%), Query Frame = 0

Query: 21  FFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASKCVRSLYTDQFKL 80
           FF+L+I+    +      ++G+ CSS+ +C  GL C SC       S C R    +    
Sbjct: 6   FFNLMIIALCLSSFVHSLEMGETCSSTSECDAGLSCQSCPANGNTGSTCTRIQPLNPTSK 65

Query: 81  LNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNNGVRAFMLDTYDF 140
           + N L FNKY++LTTHNSYAI G  S TG   V+  NQED+I+ QL NGVR  MLDTYDF
Sbjct: 66  V-NGLPFNKYSWLTTHNSYAITGANSATGSFLVSPKNQEDSITNQLKNGVRGIMLDTYDF 125

Query: 141 LGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILEDYVQSPNGLSKVF 200
             D+WLCHS GG C+N+TAF+PA++ LK I  FL +N  EIVT+ILEDYV+S  GL+ VF
Sbjct: 126 QNDIWLCHSTGGTCFNFTAFQPAINALKEINDFLESNLSEIVTIILEDYVKSQMGLTNVF 185

Query: 201 TDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATEGIAYQWNYMVEN 260
             +GL KF  PI+ MPK+G DWP V DMV  NQRL+VFTS + KEA+EG+AYQWNYMVEN
Sbjct: 186 NASGLSKFLLPISRMPKDGTDWPTVDDMVKQNQRLVVFTSKKDKEASEGLAYQWNYMVEN 245

Query: 261 QYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNSKDLSAMLQTCHQ 320
           QYGNDGM++GSC  R+ESS+LD   +SL+  NYF T P   QAC  NS  L  M++TCH+
Sbjct: 246 QYGNDGMKDGSCSSRSESSSLDTMSRSLVFQNYFETSPNSTQACADNSSPLIEMMRTCHE 305

Query: 321 AAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
           AA  RW NF+A+DFY+RSD GGA +A+D  NG L C    +  C
Sbjct: 306 AAGKRWPNFIAVDFYQRSDSGGAAEAVDEANGRLTCGCDSLVYC 348

BLAST of Lag0030148 vs. TAIR 10
Match: AT5G67130.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 376.3 bits (965), Expect = 2.7e-104
Identity = 177/356 (49.72%), Postives = 236/356 (66.29%), Query Frame = 0

Query: 9   KKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASK 68
           + + + L+ LL+ FS         CSNG C++ D CSS+ DC  GLYC  C         
Sbjct: 10  RAVTVSLLLLLLSFSF-----SSACSNGNCQLLDSCSSATDCVSGLYCGDCPAVGRSKPV 69

Query: 69  CVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNN 128
           C R   T    ++ N L FNKY +L THN+++    P   G+ R+T  NQEDTI+ QL N
Sbjct: 70  CTRGQATSPTSII-NGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQN 129

Query: 129 GVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILED 188
           GVR  MLD YDF  D+WLCHS  G+C+N+TAF+PA++ L+ +EAFL+ N  EIVT+I+ED
Sbjct: 130 GVRGLMLDMYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIED 189

Query: 189 YVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATE 248
           YV  P GLS +F +AGL K+WFP++ MP+ G DWP V+DMV  N RLLVFTS   KE  E
Sbjct: 190 YVHRPKGLSTLFANAGLDKYWFPVSKMPRKGEDWPTVTDMVQENHRLLVFTSVAAKEDEE 249

Query: 249 GIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNS 308
           G+AYQW YMVEN+ G+ G++ GSCP R ES  L+ K  SL L+NYFPT P +K AC+ +S
Sbjct: 250 GVAYQWRYMVENESGDPGVKRGSCPNRKESQPLNSKSSSLFLMNYFPTYPVEKDACKEHS 309

Query: 309 KDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
             L+ M+ TC ++  NR  NF+A++FY RSDGGG F+ LD +NG +LC  + + +C
Sbjct: 310 APLAEMVGTCLKSGGNRMPNFLAVNFYMRSDGGGVFEILDRMNGPVLCGCETLSAC 359

BLAST of Lag0030148 vs. TAIR 10
Match: AT3G19310.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 5.9e-99
Identity = 176/356 (49.44%), Postives = 235/356 (66.01%), Query Frame = 0

Query: 9   KKLVILLISLLIFFSLLIVEAEETCSNGLCKVGDECSSSGDCGGGLYCFSCTPTSFFASK 68
           ++    L +LLI   L+I+           K G+ C  S +C  GL+C SC  +  F  +
Sbjct: 3   QRFTFFLTALLIPCILIILSPSYA-----LKEGETCIVSKNCDRGLHCESCLASDSFRPR 62

Query: 69  CVRSLYTDQFKLLNNSLAFNKYAYLTTHNSYAIEGEPSHTGLPRVTLNNQEDTISQQLNN 128
           C R    +    +   L +NKY++LTTHNS+A  G  S TG   +  +NQ+D+I+ QL N
Sbjct: 63  CSRMQPINPTTKV-KGLPYNKYSWLTTHNSFARMGAKSGTGSMILAPSNQQDSITSQLLN 122

Query: 129 GVRAFMLDTYDFLGDVWLCHSFGGKCYNYTAFEPALDTLKVIEAFLAANTQEIVTLILED 188
           GVR FMLD YDF  D+WLCHS+GG C+NYTAF+PA++ LK  + FL  N   +VTLILED
Sbjct: 123 GVRGFMLDLYDFQNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILED 182

Query: 189 YVQSPNGLSKVFTDAGLKKFWFPITNMPKNGNDWPRVSDMVANNQRLLVFTSNRTKEATE 248
           YV+SPNGL++VF  +GL+ F FP++ MPKNG DWP + DM+  NQRLLVFTSN  KEA+E
Sbjct: 183 YVKSPNGLTRVFDASGLRNFMFPVSRMPKNGEDWPTLDDMICQNQRLLVFTSNPQKEASE 242

Query: 249 GIAYQWNYMVENQYGNDGMQEGSCPKRAESSALDDKGKSLILVNYFPTVPFKKQACEHNS 308
           GIA+ W YM+ENQYG+ GM+ G C  R ES A+ D+ +SLILVNYFP       +C+ NS
Sbjct: 243 GIAFMWRYMIENQYGDGGMKAGVCTNRPESVAMGDRSRSLILVNYFPDTADVIGSCKQNS 302

Query: 309 KDLSAMLQTCHQAAANRWANFVALDFYKRSDGGGAFQALDTLNGELLCASKDIHSC 365
             L   ++ C +A+  RW NF+A+DFYKRSDGGGA +A+D  NG  +C  +DI +C
Sbjct: 303 APLLDTVKNCQEASGKRWPNFIAVDFYKRSDGGGAPKAVDVANGHAVCGCEDIAAC 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023549235.15.6e-18486.61PI-PLC X domain-containing protein At5g67130 [Cucurbita pepo subsp. pepo][more]
XP_022953911.11.4e-18286.07PI-PLC X domain-containing protein At5g67130 [Cucurbita moschata][more]
XP_022990547.13.1e-18286.07PI-PLC X domain-containing protein At5g67130 [Cucurbita maxima][more]
KAG7033214.13.1e-18285.79PI-PLC X domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602537.15.3e-18285.79PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q93XX53.9e-10349.72PI-PLC X domain-containing protein At5g67130 OS=Arabidopsis thaliana OX=3702 GN=... [more]
D4AUX61.2e-0624.14Uncharacterized secreted protein ARB_08043 OS=Arthroderma benhamiae (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A6J1GPE36.7e-18386.07PI-PLC X domain-containing protein At5g67130 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JJ271.5e-18286.07PI-PLC X domain-containing protein At5g67130 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1C0L76.1e-17682.24PI-PLC X domain-containing protein At5g67130 OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7TBL43.0e-17585.11PI-PLC X domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C9K66.7e-17585.07PI-PLC X domain-containing protein At5g67130 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
AT1G13680.19.4e-12162.24PLC-like phosphodiesterases superfamily protein [more]
AT1G49740.14.5e-10752.94PLC-like phosphodiesterases superfamily protein [more]
AT4G36945.11.0e-10654.36PLC-like phosphodiesterases superfamily protein [more]
AT5G67130.12.7e-10449.72PLC-like phosphodiesterases superfamily protein [more]
AT3G19310.15.9e-9949.44PLC-like phosphodiesterases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilyGENE3D3.20.20.190Phosphatidylinositol (PI) phosphodiesterasecoord: 74..352
e-value: 5.7E-28
score: 100.2
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 82..352
NoneNo IPR availablePANTHERPTHR13593UNCHARACTERIZEDcoord: 24..365
NoneNo IPR availablePANTHERPTHR13593:SF51F21F23.12 PROTEINcoord: 24..365
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 87..194
score: 9.6846
NoneNo IPR availableCDDcd08588PI-PLCc_At5g67130_likecoord: 82..351
e-value: 1.76252E-102
score: 301.556

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0030148.1Lag0030148.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0008081 phosphoric diester hydrolase activity