Lag0029482 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029482
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NAD(P)H dehydrogenase (quinone)
Location	chr8: 39397533 .. 39397859 (+)
RNA-Seq Expression	Lag0029482
Synteny	Lag0029482

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTACTGGCAGCACCAGTGAAGAATGCAATAGACTGGGGATCAAGGCCACCAAATGTATGGGGAGACAAAGCAGCAGCAATCGTGAGTGCAAGTGGGGGCTCCGGCGGAGGACGATCACAGTACCATCTTCGCCAAATTGGAGTGTTTCTTGACCTTCATTTCATAAACAAGCCTGAGTTTTTCTTGAACGCGCACCAGCCGCCGCCCAAGTTCGACAGCACCGGCGACTTGATCGACCGCGAAGTTCAAGAGAGGTTGAAGGCTGTTCTTCTGGCCTTGCGTGCGTTTGCCTTGAGACTACGAGGAACACAACAATCTCTGTGA

mRNA sequence

ATGTTACTGGCAGCACCAGTGAAGAATGCAATAGACTGGGGATCAAGGCCACCAAATGTATGGGGAGACAAAGCAGCAGCAATCGTGAGTGCAAGTGGGGGCTCCGGCGGAGGACGATCACAGTACCATCTTCGCCAAATTGGAGTGTTTCTTGACCTTCATTTCATAAACAAGCCTGAGTTTTTCTTGAACGCGCACCAGCCGCCGCCCAAGTTCGACAGCACCGGCGACTTGATCGACCGCGAAGTTCAAGAGAGGTTGAAGGCTGTTCTTCTGGCCTTGCGTGCGTTTGCCTTGAGACTACGAGGAACACAACAATCTCTGTGA

Coding sequence (CDS)

ATGTTACTGGCAGCACCAGTGAAGAATGCAATAGACTGGGGATCAAGGCCACCAAATGTATGGGGAGACAAAGCAGCAGCAATCGTGAGTGCAAGTGGGGGCTCCGGCGGAGGACGATCACAGTACCATCTTCGCCAAATTGGAGTGTTTCTTGACCTTCATTTCATAAACAAGCCTGAGTTTTTCTTGAACGCGCACCAGCCGCCGCCCAAGTTCGACAGCACCGGCGACTTGATCGACCGCGAAGTTCAAGAGAGGTTGAAGGCTGTTCTTCTGGCCTTGCGTGCGTTTGCCTTGAGACTACGAGGAACACAACAATCTCTGTGA

Protein sequence

MLLAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFFLNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRGTQQSL

Homology

[Close]

BLAST of Lag0029482 vs. NCBI nr
Match: XP_004140288.1 (NAD(P)H:quinone oxidoreductase [Cucumis sativus] >KGN51093.1 hypothetical protein Csa_008941 [Cucumis sativus])

HSP 1 Score: 189.5 bits (480), Expect = 1.5e-44
Identity = 90/102 (88.24%), Postives = 98/102 (96.08%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           + AP+KNAIDWGSRPPNVWGDKAAAIVSASGGSGG RSQYHLRQIGVFLDLHFINKPEFF
Sbjct: 98  VTAPLKNAIDWGSRPPNVWGDKAAAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEFF 157

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRGT 105
           LNAHQPPPKF+S+GDLID EV+ RLKA+LLALRAF+LRL+GT
Sbjct: 158 LNAHQPPPKFNSSGDLIDAEVRARLKALLLALRAFSLRLKGT 199

[Close]

BLAST of Lag0029482 vs. NCBI nr
Match: XP_038876071.1 (LOW QUALITY PROTEIN: NAD(P)H:quinone oxidoreductase-like [Benincasa hispida])

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-43
Identity = 88/103 (85.44%), Postives = 96/103 (93.20%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           + AP+KNAIDWGSRPPNVWGDKAAAIVSASGGS G RSQYHL QIGVFLDLHFINKPEFF
Sbjct: 99  VTAPLKNAIDWGSRPPNVWGDKAAAIVSASGGSSGVRSQYHLXQIGVFLDLHFINKPEFF 158

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRGTQ 106
           LNAHQPPPKFD  GDLID EV+E+LKA+LLALRAF+LRL+GT+
Sbjct: 159 LNAHQPPPKFDGNGDLIDAEVREKLKALLLALRAFSLRLKGTK 201

[Close]

BLAST of Lag0029482 vs. NCBI nr
Match: KAG6601943.1 (NADPH:quinone oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 185.7 bits (470), Expect = 2.2e-43
Identity = 87/102 (85.29%), Postives = 97/102 (95.10%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           + AP+KNA+DW SRPPNVWG+KAAAIVS+SGGSGG RSQYHLRQIGVFLDLHFINKPEFF
Sbjct: 161 VTAPLKNALDWASRPPNVWGNKAAAIVSSSGGSGGARSQYHLRQIGVFLDLHFINKPEFF 220

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRGT 105
           LNA+QPPPKFDS GDLIDR+V+E+LKAVLLAL AF+LRLRGT
Sbjct: 221 LNAYQPPPKFDSHGDLIDRQVREKLKAVLLALHAFSLRLRGT 262

[Close]

BLAST of Lag0029482 vs. NCBI nr
Match: XP_023525728.1 (NAD(P)H:quinone oxidoreductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 185.3 bits (469), Expect = 2.8e-43
Identity = 87/102 (85.29%), Postives = 96/102 (94.12%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           + AP+KNA+DW SRPPNVWG+KAAAIVS+SGGSGG RSQYHLRQIGVFLDLHFINKPEFF
Sbjct: 93  VTAPLKNALDWASRPPNVWGNKAAAIVSSSGGSGGARSQYHLRQIGVFLDLHFINKPEFF 152

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRGT 105
           LNA+QPPPKFDS GDLIDREV+E+LKAVL AL AF+LRLRGT
Sbjct: 153 LNAYQPPPKFDSHGDLIDREVREKLKAVLFALHAFSLRLRGT 194

[Close]

BLAST of Lag0029482 vs. NCBI nr
Match: KAA0050199.1 (NAD(P)H:quinone oxidoreductase [Cucumis melo var. makuwa] >TYJ98211.1 NAD(P)H:quinone oxidoreductase [Cucumis melo var. makuwa])

HSP 1 Score: 184.5 bits (467), Expect = 4.9e-43
Identity = 88/101 (87.13%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 2   LLAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEF 61
           LL AP+KNAIDWGSRPPNVWGDKAAAIVSASGGSGG RSQYHLRQIGVFLDLHFINKPEF
Sbjct: 3   LLLAPLKNAIDWGSRPPNVWGDKAAAIVSASGGSGGVRSQYHLRQIGVFLDLHFINKPEF 62

Query: 62  FLNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLR 103
           FLNAHQPPPKFDS+GDLID EV+ +LK +LLAL AF+LRL+
Sbjct: 63  FLNAHQPPPKFDSSGDLIDAEVRAKLKTLLLALHAFSLRLK 103

[Close]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot
Match: Q8H9D2 (NAD(P)H:quinone oxidoreductase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 4.0e-40
Identity = 78/101 (77.23%), Postives = 87/101 (86.14%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           +  P+KNAIDW SRPPNVW DKAAA+VSA GG GGGRSQYHLRQIGVFLDLHFINKPEFF
Sbjct: 91  ITGPLKNAIDWASRPPNVWADKAAAMVSAGGGFGGGRSQYHLRQIGVFLDLHFINKPEFF 150

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRG 104
           LNA Q PPKFDS G L D E ++RL+AVLLAL+A AL+L+G
Sbjct: 151 LNAFQQPPKFDSDGVLTDEETKQRLRAVLLALQALALKLKG 191

[Close]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot
Match: Q941Y8 (Probable NADPH:quinone oxidoreductase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0954000 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.5e-39
Identity = 73/101 (72.28%), Postives = 89/101 (88.12%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           ++ P+KNA+DWGSRPPN W D+AAAIVSASGGSGG RS YH+RQ+GVFLD+HFINKPE F
Sbjct: 94  ISGPLKNALDWGSRPPNCWADRAAAIVSASGGSGGSRSMYHIRQVGVFLDIHFINKPEVF 153

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRG 104
           + AHQPP KFDS G+LID E++E LK +LL+L+AFALRL+G
Sbjct: 154 IKAHQPPKKFDSDGNLIDPEIKEELKDMLLSLQAFALRLQG 194

[Close]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot
Match: Q9LK88 (NADPH:quinone oxidoreductase OS=Arabidopsis thaliana OX=3702 GN=NQR PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.3e-38
Identity = 74/101 (73.27%), Postives = 86/101 (85.15%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           ++AP+KNA+DW SRPPNVW DK AAI+S  GG GGGRSQYHLRQIGVFLDLHFINKPEF 
Sbjct: 95  VSAPLKNALDWASRPPNVWADKPAAIISTGGGFGGGRSQYHLRQIGVFLDLHFINKPEFT 154

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRG 104
           LNA QPP KFD+ G+L+D   +ERLK VLL+L+AF LRL+G
Sbjct: 155 LNAFQPPQKFDAEGNLVDEVTKERLKQVLLSLQAFTLRLQG 195

[Close]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot
Match: Q941Z0 (Probable NADPH:quinone oxidoreductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0953600 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.1e-34
Identity = 68/100 (68.00%), Postives = 82/100 (82.00%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPPNVWGDKAAAIVSASGGSGGGRSQYHLRQIGVFLDLHFINKPEFF 62
           +A P+KNA+DW SR  N W DK AAIVSA GG GGGRSQYHLRQ+GVFLDLHFINKPE  
Sbjct: 95  IATPLKNALDWASRGQNCWADKPAAIVSAGGGFGGGRSQYHLRQVGVFLDLHFINKPELA 154

Query: 63  LNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLR 103
           + A + PPKFDS G+LID +++ER+K VLL+L+AF LRL+
Sbjct: 155 VKAFEQPPKFDSDGNLIDAQIRERIKQVLLSLQAFTLRLQ 194

[Close]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot
Match: Q9I4D4 (NAD(P)H-dependent FMN reductase PA1204 OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA1204 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 9.0e-16
Identity = 49/103 (47.57%), Postives = 67/103 (65.05%), Query Frame = 0


Query: 3   LAAPVKNAIDWGSRPP-NVWGDKAAAIVSASGGS-GGGRSQYHLRQIGVFLDLHFINKPE 62
           +A  +KNAIDW SRPP   +  K AAI+ AS G  G  R+QYHLRQ  VFLD+H +NKPE
Sbjct: 85  MAGVLKNAIDWASRPPEQPFSGKPAAILGASAGRFGTARAQYHLRQTLVFLDVHPLNKPE 144

Query: 63  FFLNAHQPPPKFDSTGDLIDREVQERLKAVLLALRAFALRLRG 104
             +++ Q    FD+ G L+D + +E ++  L AL+ +  RLRG
Sbjct: 145 VMISSAQ--NAFDAQGRLLDDKARELIQQQLQALQLWVRRLRG 185

The following BLAST results are available for this feature:

BLAST of Lag0029482 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140288.1	1.5e-44	88.24	NAD(P)H:quinone oxidoreductase [Cucumis sativus] >KGN51093.1 hypothetical protei...	[more]
XP_038876071.1	1.3e-43	85.44	LOW QUALITY PROTEIN: NAD(P)H:quinone oxidoreductase-like [Benincasa hispida]	[more]
KAG6601943.1	2.2e-43	85.29	NADPH:quinone oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023525728.1	2.8e-43	85.29	NAD(P)H:quinone oxidoreductase-like [Cucurbita pepo subsp. pepo]	[more]
KAA0050199.1	4.9e-43	87.13	NAD(P)H:quinone oxidoreductase [Cucumis melo var. makuwa] >TYJ98211.1 NAD(P)H:qu...	[more]

BLAST of Lag0029482 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H9D2	4.0e-40	77.23	NAD(P)H:quinone oxidoreductase OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q941Y8	1.5e-39	72.28	Probable NADPH:quinone oxidoreductase 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9LK88	1.3e-38	73.27	NADPH:quinone oxidoreductase OS=Arabidopsis thaliana OX=3702 GN=NQR PE=1 SV=1	[more]
Q941Z0	1.1e-34	68.00	Probable NADPH:quinone oxidoreductase 1 OS=Oryza sativa subsp. japonica OX=39947...	[more]
Q9I4D4	9.0e-16	47.57	NAD(P)H-dependent FMN reductase PA1204 OS=Pseudomonas aeruginosa (strain ATCC 15...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029039	Flavoprotein-like superfamily	GENE3D	3.40.50.360		coord: 3..108 e-value: 5.1E-25 score: 90.0
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	52218	Flavoproteins	coord: 5..92
IPR005025	NADPH-dependent FMN reductase-like	PFAM	PF03358	FMN_red	coord: 5..64 e-value: 1.4E-10 score: 41.1
None	No IPR available	PANTHER	PTHR30543:SF26	OXIDOREDUCTASE, PUTATIVE-RELATED	coord: 3..102
None	No IPR available	PANTHER	PTHR30543	CHROMATE REDUCTASE	coord: 3..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029482.1	Lag0029482.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0050136	NADH dehydrogenase (quinone) activity
molecular_function	GO:0008753	NADPH dehydrogenase (quinone) activity
molecular_function	GO:0016491	oxidoreductase activity