Lag0029240 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029240
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Aspartic proteinase nepenthesin-1
Location	chr8: 36825142 .. 36825750 (-)
RNA-Seq Expression	Lag0029240
Synteny	Lag0029240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATTCACAAATTTTCTTACTGTATGGAACTGGGAGTCTGAATTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCGAGGTCACGCCACTGATACAAAATCCATTTGACCCATCTTTCTACTATCTATCCCTACAAGGAATCACAGTAGCCCAGAAGTTCTTGCTCATCCCGTCCTCGTGGTTTAGACTAAACACCGATGGCAGCGGCGGCGTGATTATTGATTCTGGCACATCAATCACCACTTAACAGAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAAGCATTGGTCTTCATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGCCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCTCACAGTGTGCTCGGCTTGGTAA

mRNA sequence

ATGACATTCACAAATTTTCTTACTGTATGGAACTGGGAGTCTGAATTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCGAGAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAAGCATTGGTCTTCATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGCCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCTCACAGTGTGCTCGGCTTGGTAA

Coding sequence (CDS)

ATGACATTCACAAATTTTCTTACTGTATGGAACTGGGAGTCTGAATTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCGAGAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAAGCATTGGTCTTCATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCGACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGCCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCTCACAGTGTGCTCGGCTTGGTAA

Protein sequence

MTFTNFLTVWNWESEFRIFNKYRQHYESSHREDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPVENYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVCSAW

Homology

[Close]

BLAST of Lag0029240 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 179.1 bits (453), Expect = 2.7e-41
Identity = 88/112 (78.57%), Postives = 96/112 (85.71%), Query Frame = 0


Query: 32  EDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPVE 91
           ED FDVLKP F +QTNL V  S SIGL LCF+LPSHN SKLDVPDL+FHFE LDLKLPVE
Sbjct: 318 EDVFDVLKPVFASQTNLQVIWS-SIGLDLCFQLPSHNNSKLDVPDLIFHFEDLDLKLPVE 377

Query: 92  NYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVCS 144
           NYMV +EE G+VCL MGAAG LSIFGSMQHQNMLVLHDL+K+VVSF+ T CS
Sbjct: 378 NYMVVNEELGIVCLAMGAAGGLSIFGSMQHQNMLVLHDLEKKVVSFIPTQCS 428

[Close]

BLAST of Lag0029240 vs. NCBI nr
Match: XP_010664223.1 (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 145.6 bits (366), Expect = 3.4e-31
Identity = 69/112 (61.61%), Postives = 86/112 (76.79%), Query Frame = 0


Query: 31  REDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPV 90
           +EDAFDVLK  F +QT L V +S++ GL LCF LP  N +++ VP L+FHF+GLDL LPV
Sbjct: 332 QEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPV 391

Query: 91  ENYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVC 143
           ENYMV+D E GL+CL + A G+LSIFG++Q QNMLVLHDLKK  +S V T C
Sbjct: 392 ENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHDLKKSTLSLVPTQC 443

[Close]

BLAST of Lag0029240 vs. NCBI nr
Match: RVX22508.1 (Aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 145.6 bits (366), Expect = 3.4e-31
Identity = 69/112 (61.61%), Postives = 86/112 (76.79%), Query Frame = 0


Query: 31  REDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPV 90
           +EDAFDVLK  F +QT L V +S++ GL LCF LP  N +++ VP L+FHF+GLDL LPV
Sbjct: 332 QEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPV 391

Query: 91  ENYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVC 143
           ENYMV+D E GL+CL + A G+LSIFG++Q QNMLVLHDLKK  +S V T C
Sbjct: 392 ENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHDLKKSTLSLVPTQC 443

[Close]

BLAST of Lag0029240 vs. NCBI nr
Match: CBI19032.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 145.6 bits (366), Expect = 3.4e-31
Identity = 69/112 (61.61%), Postives = 86/112 (76.79%), Query Frame = 0


Query: 31  REDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPV 90
           +EDAFDVLK  F +QT L V +S++ GL LCF LP  N +++ VP L+FHF+GLDL LPV
Sbjct: 268 QEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPV 327

Query: 91  ENYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVC 143
           ENYMV+D E GL+CL + A G+LSIFG++Q QNMLVLHDLKK  +S V T C
Sbjct: 328 ENYMVSDPEMGLICLAIDATGSLSIFGNIQQQNMLVLHDLKKSTLSLVPTQC 379

[Close]

BLAST of Lag0029240 vs. NCBI nr
Match: XP_034676400.1 (aspartic proteinase nepenthesin-1-like [Vitis riparia])

HSP 1 Score: 141.7 bits (356), Expect = 4.8e-30
Identity = 67/112 (59.82%), Postives = 85/112 (75.89%), Query Frame = 0


Query: 31  REDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPV 90
           +EDAFDVLK  F +QT L V +S++ GL LCF LP  N +++ VP L+FHF+GLDL LPV
Sbjct: 332 QEDAFDVLKNAFISQTELQVANSSTTGLDLCFHLPVKNAAEVKVPKLIFHFKGLDLALPV 391

Query: 91  ENYMVTDEERGLVCLPMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVC 143
           ENYMV+D + GL+CL + A G+ SIFG++Q QNMLVLHDLKK  +S V T C
Sbjct: 392 ENYMVSDPDMGLICLAIDATGSPSIFGNIQQQNMLVLHDLKKSTLSLVPTQC 443

[Close]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 9.5e-21
Identity = 51/113 (45.13%), Postives = 73/113 (64.60%), Query Frame = 0


Query: 33  DAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPVEN 92
           +A+  ++  F +Q NLPV + +S G  LCF+ PS + S L +P  V HF+G DL+LP EN
Sbjct: 326 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPS-DPSNLQIPTFVMHFDGGDLELPSEN 385

Query: 93  YMVTDEERGLVCLPMGAAG-ALSIFGSMQHQNMLVLHDLKKEVVSFVLTVCSA 145
           Y ++    GL+CL MG++   +SIFG++Q QNMLV++D    VVSF    C A
Sbjct: 386 YFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCGA 436

[Close]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 47/115 (40.87%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 32  EDAFDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPVE 91
           +DA++ +   FT Q NLP    +S GL  CF+ PS + S + VP++   F+G  L L  +
Sbjct: 325 QDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPS-DGSTVQVPEISMQFDGGVLNLGEQ 384

Query: 92  NYMVTDEERGLVCLPMGAAGAL--SIFGSMQHQNMLVLHDLKKEVVSFVLTVCSA 145
           N +++  E G++CL MG++  L  SIFG++Q Q   VL+DL+   VSFV T C A
Sbjct: 385 NILISPAE-GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCGA 437

[Close]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-11
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 35  FDVLKPFFTAQTNLPVKHSASIGLHLCFELPSHNK-SKLDVPDLVFHFEGLDLKLPVENY 94
           +D L      +  LP    +S+GL LCF LP      ++ VP +   F+G  L+L     
Sbjct: 350 YDELVNDLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARL 409

Query: 95  MVTDEERGLVCLPMG--AAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVCSA 145
              D E G++CL +G   AG++SI G+ Q QNM VL++L++  V+FV + C A
Sbjct: 410 FAEDRESGMMCLMVGRAEAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPCGA 462

[Close]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.7e-09
Identity = 32/84 (38.10%), Postives = 45/84 (53.57%), Query Frame = 0


Query: 61  CFELPSHNKSKLDVPDLVFHFEGLDLKLPVENYMVTDEERGLVCLPM-GAAGALSIFGSM 120
           CF+L + N+ K  VP +V HF G D+ LP  NY++  +  G  C    G  G LSI G++
Sbjct: 404 CFDLSNMNEVK--VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNI 463

Query: 121 QHQNMLVLHDLKKEVVSFVLTVCS 144
           Q Q   V++DL    V F    C+
Sbjct: 464 QQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.5e-05
Identity = 30/118 (25.42%), Postives = 53/118 (44.92%), Query Frame = 0


Query: 32  EDAFDVLKPFFTAQTNLPVKHSASIG-LHLCFELPSHNKSKLDVPDLVFHFEGLDLKLPV 91
           +  ++ ++  F  Q N  V   +++G    CF   + N +    P +  H   LDLKLP+
Sbjct: 338 QPVYEAIRDEFRKQVN--VSSFSTLGAFDTCFSADNENVA----PKITLHMTSLDLKLPM 397

Query: 92  ENYMVTDEERGLVCLPM-----GAAGALSIFGSMQHQNMLVLHDLKKEVVSFVLTVCS 144
           EN ++      L CL M      A   L++  ++Q QN+ +L D+    +      C+
Sbjct: 398 ENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449

The following BLAST results are available for this feature:

BLAST of Lag0029240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886455.1	2.7e-41	78.57	aspartic proteinase nepenthesin-2-like [Benincasa hispida]	[more]
XP_010664223.1	3.4e-31	61.61	PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]
RVX22508.1	3.4e-31	61.61	Aspartic proteinase nepenthesin-1 [Vitis vinifera]	[more]
CBI19032.3	3.4e-31	61.61	unnamed protein product, partial [Vitis vinifera]	[more]
XP_034676400.1	4.8e-30	59.82	aspartic proteinase nepenthesin-1-like [Vitis riparia]	[more]

BLAST of Lag0029240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	9.5e-21	45.13	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.2e-15	40.87	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q7XV21	2.3e-11	36.28	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q9LNJ3	3.7e-09	38.10	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
O04496	1.5e-05	25.42	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 29..145 e-value: 3.4E-21 score: 77.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 31..142
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 31..137 e-value: 3.8E-14 score: 52.8
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 31..143
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 31..143
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 1..138 score: 10.018705

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029240.1	Lag0029240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0008233	peptidase activity