Lag0028062 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028062
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	receptor-like serine/threonine-protein kinase ALE2 isoform X1
Location	chr8: 12103119 .. 12104525 (+)
RNA-Seq Expression	Lag0028062
Synteny	Lag0028062

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCTTCTCATGTTCTTGTGTGGAGGCAACGACGGGGAAGACGGTGGTGGTAGTGTCGTATGTGGTGGATGTTCCCTCCAGCAGTTCTAAAGAAGATACAGTTTTGTTTGCCGATACCATTGTCAAATGTAATCTCAAGTGGTTGGCTCGTACGGCAGAGACGCTTCTCTGAAATGCGACTTCTTCTTCTTTTTTGTTTTTCATTTTGCTTTATTAGGTCGGTTGCTTTTGCTTTTTTTTTTTTTTGTTAAAATAGAAAGTGTCGCTATTTTTAAGTAAACCTCTCGTTGTTTAATATTTTTAATTCTTAATTAATTCTTTATATAAAAAGGAGGGTTGAAATTCGTTCAAAGAAAGTTGTTTTTTTTTTAAACCATTCAAAGGGAGTTGCTGAAATGTTTTCACGGAAGCAATAGTAACTCTTCCATGAAAGTTCAAACACCAGCACTGAAGTTGTTCGTGCCACTGGGGAGTCTTGTTTCTTATTCTTTGCTTTCTTTGTTTATCCTTGAATCTCATTCTCTTCTAGCTACGACTTTTTTTCCACTGTGAAGCTTTGCGATTCTTTCAATTGATTTTACAATTTCAAGTGTTGAAATTATGCTATGTTTTTTGTCGTTGTGAATGCATCTTGTGTATCTGATATTTCTGTTGATGGTGGTTTTGATGTTGATGTCTTTTTTTGTTACTCTTGCTGAAGTTGAAAGTGAAACCTAGTTGGAAACTGTGTGTGGGTGGACTGATGTTTGTGAGATATTGTGATGTTCTGAACTATTTGTGTTTCTTAATTTATCAATTTGTTCATTGGTGTTCTAAATAATTGTGCTGGTTTGGCCACTTCCCATGTTGTAGGATTCAGCAACTCTCCACCCCCTTCAACTTTTTCTGCATTTCCTCTAGTTGGAGGAACACCTGGTCCAGTCCAACATGATAAGTCATTGATAAGCAATGGACTAAGCACAGCGCCCCAACAGAATGGTAGAATCCATAAACTTGGTAACTTTTACATTGTTATTTCTTCAGACTGTTAACATTCCCATTTTGACAATGTAGAGTCTACTTCGCAATCACCACTCATGTTACCGCCAACTGCACCAGTTCCATTGCCTGACACGATCAAAGGGTTTATACCGTCCGCATCACCTGGTGCTTCGACTGAGTTGCCACCATTTAATAGCATGCCTCATCCACCTTTACTAGTTCAACCAAATGCACCATTGATTTCACCAAGTGCACCAGAAGCAGAAGGTAAAACACCAATGAATGAGACTCCTCTGCCAATGGATTCTCCAGGTAGATAACTTAACTGGTCTCCTGCATTTTCGTGTTAAATTGATTTATCATTTGCTTAACTGACCCTTTTTCTTCTAACCAAGTAGGAATTCGAGAACAAGATGCAAAGTGA

mRNA sequence

ATGATCTTCTCATGTTCTTGTGTGGAGGCAACGACGGGGAAGACGGTGGTGGTAGTGTCGTATGTGGTGGATGTTCCCTCCAGCAGTTCTAAAGAAGATACAGTTTTGTTTGCCGATACCATTGTCAAATGTAATCTCAAGTGGTTGGCTCGATTCAGCAACTCTCCACCCCCTTCAACTTTTTCTGCATTTCCTCTAGTTGGAGGAACACCTGGTCCAGTCCAACATGATAAGTCATTGATAAGCAATGGACTAAGCACAGCGCCCCAACAGAATGAGTCTACTTCGCAATCACCACTCATGTTACCGCCAACTGCACCAGTTCCATTGCCTGACACGATCAAAGGGTTTATACCGTCCGCATCACCTGGTGCTTCGACTGAGTTGCCACCATTTAATAGCATGCCTCATCCACCTTTACTAGTTCAACCAAATGCACCATTGATTTCACCAAGTGCACCAGAAGCAGAAGGTAAAACACCAATGAATGAGACTCCTCTGCCAATGGATTCTCCAGGAATTCGAGAACAAGATGCAAAGTGA

Coding sequence (CDS)

ATGATCTTCTCATGTTCTTGTGTGGAGGCAACGACGGGGAAGACGGTGGTGGTAGTGTCGTATGTGGTGGATGTTCCCTCCAGCAGTTCTAAAGAAGATACAGTTTTGTTTGCCGATACCATTGTCAAATGTAATCTCAAGTGGTTGGCTCGATTCAGCAACTCTCCACCCCCTTCAACTTTTTCTGCATTTCCTCTAGTTGGAGGAACACCTGGTCCAGTCCAACATGATAAGTCATTGATAAGCAATGGACTAAGCACAGCGCCCCAACAGAATGAGTCTACTTCGCAATCACCACTCATGTTACCGCCAACTGCACCAGTTCCATTGCCTGACACGATCAAAGGGTTTATACCGTCCGCATCACCTGGTGCTTCGACTGAGTTGCCACCATTTAATAGCATGCCTCATCCACCTTTACTAGTTCAACCAAATGCACCATTGATTTCACCAAGTGCACCAGAAGCAGAAGGTAAAACACCAATGAATGAGACTCCTCTGCCAATGGATTCTCCAGGAATTCGAGAACAAGATGCAAAGTGA

Protein sequence

MIFSCSCVEATTGKTVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFSNSPPPSTFSAFPLVGGTPGPVQHDKSLISNGLSTAPQQNESTSQSPLMLPPTAPVPLPDTIKGFIPSASPGASTELPPFNSMPHPPLLVQPNAPLISPSAPEAEGKTPMNETPLPMDSPGIREQDAK

Homology

[Close]

BLAST of Lag0028062 vs. NCBI nr
Match: XP_038879440.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X1 [Benincasa hispida])

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-38
Identity = 96/149 (64.43%), Postives = 105/149 (70.47%), Query Frame = 0


Query: 33  DTVLFADTIVKC--------NLKWLARFSNSPPPSTFSAFPLVGGTPGPVQHDKSLISNG 92
           + VL    ++ C        NL  +A   NSPPPSTFSAFP V GTPG VQ +KSL  NG
Sbjct: 2   EEVLILQMVILCLIIAANASNLGEIAGLRNSPPPSTFSAFPPVLGTPGSVQQEKSLRING 61

Query: 93  LSTAPQQNESTSQSPLMLPPTAPVPLPDTIKGFIPSASPGASTELPPFNSMPHPPLLVQP 152
            S+APQQNEST Q P+MLP   P PLPDTI+GFIPSASP A  E PPFNSMP P L VQP
Sbjct: 62  PSSAPQQNESTLQPPVMLPTIVPGPLPDTIEGFIPSASPHAPPEAPPFNSMPQPSLPVQP 121

Query: 153 NAPLISPSAPEAEGKTPMNETPLPMDSPG 174
           NAPLISPSAPE EGK P NETPL + SPG
Sbjct: 122 NAPLISPSAPEPEGKAPANETPLSIVSPG 150

[Close]

BLAST of Lag0028062 vs. NCBI nr
Match: XP_022147701.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X1 [Momordica charantia])

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-31
Identity = 82/125 (65.60%), Postives = 91/125 (72.80%), Query Frame = 0


Query: 49  LARFSNSPPPSTFSAFPLVGGTPGPVQHDKSLISNGLSTAPQQNESTSQSPLML-PPTAP 108
           +A F NSPP    SAFP + G PG VQH       G S+APQ NEST Q P++L PP AP
Sbjct: 26  IAGFRNSPPLVASSAFPPIEGVPGFVQH-----GTGPSSAPQPNESTLQPPVVLPPPIAP 85

Query: 109 VPLPDTIKGFIPSASPGASTELPPFNSMPHPPLLVQPNAPLISPSAPEAEGKTPMNETPL 168
           VPLPDT +GF+PS+SP   TELPPFNSMPHP L VQPNAPLISPS PEAEGK P+NETPL
Sbjct: 86  VPLPDTTEGFVPSSSPSTPTELPPFNSMPHPSLPVQPNAPLISPSTPEAEGKAPVNETPL 145

Query: 169 PMDSP 173
              SP
Sbjct: 146 SKVSP 145

[Close]

BLAST of Lag0028062 vs. NCBI nr
Match: XP_038879441.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Benincasa hispida])

HSP 1 Score: 146.0 bits (367), Expect = 3.2e-31
Identity = 87/149 (58.39%), Postives = 95/149 (63.76%), Query Frame = 0


Query: 33  DTVLFADTIVKC--------NLKWLARFSNSPPPSTFSAFPLVGGTPGPVQHDKSLISNG 92
           + VL    ++ C        NL  +A   NSPPPSTFSAFP V GTPG VQ +KSL  NG
Sbjct: 2   EEVLILQMVILCLIIAANASNLGEIAGLRNSPPPSTFSAFPPVLGTPGSVQQEKSLRING 61

Query: 93  LSTAPQQNESTSQSPLMLPPTAPVPLPDTIKGFIPSASPGASTELPPFNSMPHPPLLVQP 152
            S+APQQN                PLPDTI+GFIPSASP A  E PPFNSMP P L VQP
Sbjct: 62  PSSAPQQNG---------------PLPDTIEGFIPSASPHAPPEAPPFNSMPQPSLPVQP 121

Query: 153 NAPLISPSAPEAEGKTPMNETPLPMDSPG 174
           NAPLISPSAPE EGK P NETPL + SPG
Sbjct: 122 NAPLISPSAPEPEGKAPANETPLSIVSPG 135

[Close]

BLAST of Lag0028062 vs. NCBI nr
Match: XP_038879442.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X3 [Benincasa hispida])

HSP 1 Score: 143.3 bits (360), Expect = 2.1e-30
Identity = 77/107 (71.96%), Postives = 83/107 (77.57%), Query Frame = 0


Query: 67  VGGTPGPVQHDKSLISNGLSTAPQQNESTSQSPLMLPPTAPVPLPDTIKGFIPSASPGAS 126
           + GTPG VQ +KSL  NG S+APQQNEST Q P+MLP   P PLPDTI+GFIPSASP A 
Sbjct: 26  IAGTPGSVQQEKSLRINGPSSAPQQNESTLQPPVMLPTIVPGPLPDTIEGFIPSASPHAP 85

Query: 127 TELPPFNSMPHPPLLVQPNAPLISPSAPEAEGKTPMNETPLPMDSPG 174
            E PPFNSMP P L VQPNAPLISPSAPE EGK P NETPL + SPG
Sbjct: 86  PEAPPFNSMPQPSLPVQPNAPLISPSAPEPEGKAPANETPLSIVSPG 132

[Close]

BLAST of Lag0028062 vs. NCBI nr
Match: XP_022147702.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Momordica charantia])

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-26
Identity = 76/125 (60.80%), Postives = 86/125 (68.80%), Query Frame = 0


Query: 49  LARFSNSPPPSTFSAFPLVGGTPGPVQHDKSLISNGLSTAPQQNESTSQSPLML-PPTAP 108
           +A F NSPP    SAFP + G PG VQH       G S+APQ NEST Q P++L PP AP
Sbjct: 26  IAGFRNSPPLVASSAFPPIEGVPGFVQH-----GTGPSSAPQPNESTLQPPVVLPPPIAP 85

Query: 109 VPLPDTIKGFIPSASPGASTELPPFNSMPHPPLLVQPNAPLISPSAPEAEGKTPMNETPL 168
           VPLPDT +GF+PS+SP   TELPPFNSMPHP L VQPNAPLISPS PEAE    +  T  
Sbjct: 86  VPLPDTTEGFVPSSSPSTPTELPPFNSMPHPSLPVQPNAPLISPSTPEAEAPA-VTTTNS 144

Query: 169 PMDSP 173
           P+ SP
Sbjct: 146 PLYSP 144

[Close]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot
Match: Q9FJ49 (Abscisic acid receptor PYL12 OS=Arabidopsis thaliana OX=3702 GN=PYL12 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.4e-07
Identity = 29/46 (63.04%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 8   VEATTGKTVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFS 54
           V A   KTVVV SYVVDVP  +++E+T LFADTIV CNL+ LA+ S
Sbjct: 107 VAAEEEKTVVVESYVVDVPEGNTEEETTLFADTIVGCNLRSLAKLS 152

[Close]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot
Match: Q9FLB1 (Abscisic acid receptor PYL5 OS=Arabidopsis thaliana OX=3702 GN=PYL5 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 0


Query: 15  TVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFSN 55
           TVVV SY+VDVP  +++E+T+ F DTIV+CNL+ LAR +N
Sbjct: 162 TVVVESYIVDVPPGNTEEETLSFVDTIVRCNLQSLARSTN 201

[Close]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot
Match: Q9FJ50 (Abscisic acid receptor PYL11 OS=Arabidopsis thaliana OX=3702 GN=PYL11 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.8e-05
Identity = 28/43 (65.12%), Postives = 32/43 (74.42%), Query Frame = 0


Query: 11  TTGKTVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFS 54
           T  KTVVV SYVVDVP  +S+E+T  FADTIV  NLK LA+ S
Sbjct: 111 TEEKTVVVESYVVDVPEGNSEEETTSFADTIVGFNLKSLAKLS 153

[Close]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot
Match: Q8S8E3 (Abscisic acid receptor PYL6 OS=Arabidopsis thaliana OX=3702 GN=PYL6 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.4e-05
Identity = 24/42 (57.14%), Postives = 33/42 (78.57%), Query Frame = 0


Query: 14  KTVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFSNS 56
           +T VV SYVVDVP+ + KE+T  FADTIV+CNL+ LA+ + +
Sbjct: 169 RTRVVESYVVDVPAGNDKEETCSFADTIVRCNLQSLAKLAEN 210

[Close]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot
Match: Q9SN51 (Abscisic acid receptor PYL13 OS=Arabidopsis thaliana OX=3702 GN=PYL13 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-04
Identity = 23/40 (57.50%), Postives = 30/40 (75.00%), Query Frame = 0


Query: 14  KTVVVVSYVVDVPSSSSKEDTVLFADTIVKCNLKWLARFS 54
           KTVVV SYVVDVP  +S+EDT+ F D I++ NL  LA+ +
Sbjct: 120 KTVVVESYVVDVPEGTSEEDTIFFVDNIIRYNLTSLAKLT 159

The following BLAST results are available for this feature:

BLAST of Lag0028062 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879440.1	1.6e-38	64.43	receptor-like serine/threonine-protein kinase ALE2 isoform X1 [Benincasa hispida...	[more]
XP_022147701.1	1.4e-31	65.60	receptor-like serine/threonine-protein kinase ALE2 isoform X1 [Momordica charant...	[more]
XP_038879441.1	3.2e-31	58.39	receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Benincasa hispida...	[more]
XP_038879442.1	2.1e-30	71.96	receptor-like serine/threonine-protein kinase ALE2 isoform X3 [Benincasa hispida...	[more]
XP_022147702.1	3.1e-26	60.80	receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Momordica charant...	[more]

BLAST of Lag0028062 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ49	7.4e-07	63.04	Abscisic acid receptor PYL12 OS=Arabidopsis thaliana OX=3702 GN=PYL12 PE=1 SV=1	[more]
Q9FLB1	1.4e-05	60.00	Abscisic acid receptor PYL5 OS=Arabidopsis thaliana OX=3702 GN=PYL5 PE=1 SV=1	[more]
Q9FJ50	1.8e-05	65.12	Abscisic acid receptor PYL11 OS=Arabidopsis thaliana OX=3702 GN=PYL11 PE=1 SV=1	[more]
Q8S8E3	2.4e-05	57.14	Abscisic acid receptor PYL6 OS=Arabidopsis thaliana OX=3702 GN=PYL6 PE=1 SV=1	[more]
Q9SN51	1.2e-04	57.50	Abscisic acid receptor PYL13 OS=Arabidopsis thaliana OX=3702 GN=PYL13 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 3..61 e-value: 2.6E-13 score: 51.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..101
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 81..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 119..180
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 12..54
None	No IPR available	PANTHER	PTHR31213:SF125	ABSCISIC ACID RECEPTOR PYL4-LIKE	coord: 12..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028062.1	Lag0028062.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009738	abscisic acid-activated signaling pathway
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0080163	regulation of protein serine/threonine phosphatase activity
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0010427	abscisic acid binding
molecular_function	GO:0004864	protein phosphatase inhibitor activity
molecular_function	GO:0038023	signaling receptor activity