Lag0027975 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027975
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H
Locationchr8: 9024645 .. 9025202 (+)
RNA-Seq ExpressionLag0027975
SyntenyLag0027975
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCTGTTGAGGTGCCTTCCCGCAAGAAACGCAAAATATGTGATGAGGGAGGTACATGAAGGGGTGTGTGGAAATCAGTTAGGAGCAAAATCCCTATGTCATAAGATTAGTAGACAAGGTTACTACTGGCCGACAATGTTACAGGATGCTAAAAATTTCACGAGGTCTTATGATCAATGCCAAAGGTTTGCACCGATTCCAAGGCAACCACCAGAGCCTTTAACGAGTAGTATCAGTCCTTGGCCATTCACGCAATGGGGGATATACCTGATTGGACCTTTGCTTCAAAGTAAAGAACAGACCAAGTATGCAGTGGTGGCAGTAAATTATTTCACAAAATGGGCAAAGACAGAACCGTTATCGACTATTACAGAGAAAAAGATTGCTGATTACATTTGGAAGAATATAGTGTGTTGTTTTCGGATTCCCAACACCATTATTCAGACAATGACAGACAGTTTGACAACGCAACAACATTTAGGAAGTTTTGCTCAGACTTGGGCATCACACACCGTTGTTCATCCCCGGCTCATCCTCAAGCCAATGGGCATGTAG

mRNA sequence

ATGCCGCTGTTGAGGTGCCTTCCCGCAAGAAACGCAAAATATGTGATGAGGGAGGTACATGAAGGGGTGTGTGGAAATCAGTTAGGAGCAAAATCCCTATGTCATAAGATTAGTAGACAAGGTTACTACTGGCCGACAATGTTACAGGATGCTAAAAATTTCACGAGGTCTTATGATCAATGCCAAAGGTTTGCACCGATTCCAAGGCAACCACCAGAGCCTTTAACGAGTAGTATCAGTCCTTGGCCATTCACGCAATGGGGGATATACCTGATTGGACCTTTGCTTCAAAGTAAAGAACAGACCAAGTATGCAGTGGTGGCAGTAAATTATTTCACAAAATGGGCAAAGACAGAACCGTTATCGACTATTACAGAGAAAAAGATTGCTGATTACATTTGGAAGAATATAGTGTGTTGTTTTCGGATTCCCAACACCATTATTCAGACAATGACAGACAGTTTGACAACGCAACAACATTTAGGAAGTTTTGCTCAGACTTGGGCATCACACACCGTTGTTCATCCCCGGCTCATCCTCAAGCCAATGGGCATGTAG

Coding sequence (CDS)

ATGCCGCTGTTGAGGTGCCTTCCCGCAAGAAACGCAAAATATGTGATGAGGGAGGTACATGAAGGGGTGTGTGGAAATCAGTTAGGAGCAAAATCCCTATGTCATAAGATTAGTAGACAAGGTTACTACTGGCCGACAATGTTACAGGATGCTAAAAATTTCACGAGGTCTTATGATCAATGCCAAAGGTTTGCACCGATTCCAAGGCAACCACCAGAGCCTTTAACGAGTAGTATCAGTCCTTGGCCATTCACGCAATGGGGGATATACCTGATTGGACCTTTGCTTCAAAGTAAAGAACAGACCAAGTATGCAGTGGTGGCAGTAAATTATTTCACAAAATGGGCAAAGACAGAACCGTTATCGACTATTACAGAGAAAAAGATTGCTGATTACATTTGGAAGAATATAGTGTGTTGTTTTCGGATTCCCAACACCATTATTCAGACAATGACAGACAGTTTGACAACGCAACAACATTTAGGAAGTTTTGCTCAGACTTGGGCATCACACACCGTTGTTCATCCCCGGCTCATCCTCAAGCCAATGGGCATGTAG

Protein sequence

MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQCQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEPLSTITEKKIADYIWKNIVCCFRIPNTIIQTMTDSLTTQQHLGSFAQTWASHTVVHPRLILKPMGM
Homology
BLAST of Lag0027975 vs. NCBI nr
Match: XP_022158579.1 (uncharacterized protein LOC111025033 [Momordica charantia])

HSP 1 Score: 223.4 bits (568), Expect = 1.6e-54
Identity = 101/148 (68.24%), Postives = 118/148 (79.73%), Query Frame = 0

Query: 1   MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQ 60
           +PLL+CL    A YVMRE+HEGVCG+  GA+SL HKI RQGYYWPTML D K FT+  D+
Sbjct: 521 LPLLKCLTPDEADYVMREIHEGVCGSHSGARSLSHKIIRQGYYWPTMLNDTKXFTKKCDK 580

Query: 61  CQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEP 120
           CQRFA +PRQPPEPLTS +SPWPF QWGI LIGPL   K QTK+AVVAV+YFTKWA+ + 
Sbjct: 581 CQRFATVPRQPPEPLTSIMSPWPFAQWGIDLIGPLPLGKGQTKFAVVAVDYFTKWAEAKA 640

Query: 121 LSTITEKKIADYIWKNIVCCFRIPNTII 149
           L+TITEKK+  +IWKNI+C F IP  II
Sbjct: 641 LATITEKKVTYFIWKNIICRFGIPYAII 668

BLAST of Lag0027975 vs. NCBI nr
Match: XP_022154103.1 (uncharacterized protein LOC111021439 [Momordica charantia])

HSP 1 Score: 220.3 bits (560), Expect = 1.4e-53
Identity = 99/148 (66.89%), Postives = 118/148 (79.73%), Query Frame = 0

Query: 1   MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQ 60
           +PLL+CL    A YVMRE+HEGVCG+  GA+SL HKI RQGYYWPTML D K FT+  D+
Sbjct: 314 LPLLKCLTPDEADYVMREIHEGVCGSHSGARSLSHKIIRQGYYWPTMLNDTKQFTKKCDK 373

Query: 61  CQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEP 120
           CQRFA +PRQPPEPLTS +S WPF QWGIYLI PL   + QTK+AVVAV+YFTKWA+ + 
Sbjct: 374 CQRFATVPRQPPEPLTSIMSLWPFAQWGIYLIRPLPLGRGQTKFAVVAVDYFTKWAEAKA 433

Query: 121 LSTITEKKIADYIWKNIVCCFRIPNTII 149
           L+TITEKK+ D+IWKNI+C F I  +II
Sbjct: 434 LATITEKKVTDFIWKNIICRFGISYSII 461

BLAST of Lag0027975 vs. NCBI nr
Match: XP_024036931.1 (uncharacterized protein LOC112096932 [Citrus clementina])

HSP 1 Score: 213.8 bits (543), Expect = 1.3e-51
Identity = 96/174 (55.17%), Postives = 121/174 (69.54%), Query Frame = 0

Query: 1   MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQ 60
           +P LRCL +  AKYVMRE+HEG+CGN  GA+SL  K  RQGYYWPTM +DAKN  RS D+
Sbjct: 430 LPYLRCLGSDEAKYVMREIHEGICGNHYGAQSLAQKALRQGYYWPTMREDAKNMVRSCDK 489

Query: 61  CQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEP 120
           CQRFA +P  PPE LT   SPWPF  WG+ LIGPL   K Q K+A+VAV+YFTKWA+ EP
Sbjct: 490 CQRFARVPHLPPEKLTVISSPWPFAMWGVDLIGPLPTGKGQAKHAIVAVDYFTKWAEVEP 549

Query: 121 LSTITEKKIADYIWKNIVCCFRIPNTIIQTMTDSLTTQQHLGSFAQTWASHTVV 175
           L++ITE+K  ++IWKN++C F IP  I+    +   T  H  S  Q  A + ++
Sbjct: 550 LTSITERKTTEFIWKNLICRFGIPYAIVSDNANRFATPAHPQSNGQVEAVNKII 603

BLAST of Lag0027975 vs. NCBI nr
Match: XP_020421233.1 (uncharacterized protein LOC109949580 [Prunus persica])

HSP 1 Score: 211.1 bits (536), Expect = 8.3e-51
Identity = 98/183 (53.55%), Postives = 128/183 (69.95%), Query Frame = 0

Query: 1   MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQ 60
           +P LRCL      YV+RE+HEG+CGN  GA+SL HK  RQGY+WP++  DA+ FT+  D+
Sbjct: 725 LPYLRCLTPEEGHYVLREIHEGICGNHSGARSLAHKAIRQGYFWPSLHTDAQEFTQKCDK 784

Query: 61  CQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEP 120
           CQRFA IP+ P EPLT+ +SPWPF QWG+ LIGP+ + K Q KYAVVAV+YFTKWA+ E 
Sbjct: 785 CQRFANIPQLPAEPLTAIVSPWPFAQWGLDLIGPMPEGKCQVKYAVVAVDYFTKWAEAEA 844

Query: 121 LSTITEKKIADYIWKNIVCCFRIPNTIIQTMTDSLTTQQHLGSFAQTWASHTVVHPRLIL 180
           L+TIT  +I  ++W+NIVC F IPN+I+     +LTT  +  +F  T     V  P+ IL
Sbjct: 845 LATITAARIESFVWQNIVCRFGIPNSIVTDNGGNLTT-PNSNNFVPTSRFVCVSPPQPIL 904

Query: 181 KPM 184
            PM
Sbjct: 905 SPM 906

BLAST of Lag0027975 vs. NCBI nr
Match: TQE13472.1 (hypothetical protein C1H46_001039 [Malus baccata])

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-50
Identity = 91/148 (61.49%), Postives = 114/148 (77.03%), Query Frame = 0

Query: 1   MPLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQ 60
           +P LRCL    A+ V+RE+HEGVCG+  G++SL HK  RQGYYWPT+ QDA   +RS D+
Sbjct: 264 LPYLRCLTPAEAEIVLREIHEGVCGDHAGSRSLAHKTFRQGYYWPTLHQDAIRISRSCDK 323

Query: 61  CQRFAPIPRQPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNYFTKWAKTEP 120
           CQR+A IP  PPEPLT  ISPWPF QWG+ LIGP+   K + +YA+VAV+YFTKWA+ EP
Sbjct: 324 CQRYATIPHSPPEPLTPMISPWPFAQWGLDLIGPMPAGKGKVRYAIVAVDYFTKWAEVEP 383

Query: 121 LSTITEKKIADYIWKNIVCCFRIPNTII 149
           L+TITE KI D++WKNI+C F IPN I+
Sbjct: 384 LATITEAKIEDFVWKNILCRFGIPNAIV 411

BLAST of Lag0027975 vs. ExPASy Swiss-Prot
Match: Q9P2P1 (Protein NYNRIN OS=Homo sapiens OX=9606 GN=NYNRIN PE=2 SV=3)

HSP 1 Score: 48.5 bits (114), Expect = 9.3e-05
Identity = 37/153 (24.18%), Postives = 70/153 (45.75%), Query Frame = 0

Query: 2    PLLRCLPARNAKYVMREVHEGVCGNQLGAKSLCHKISRQGYYWPTMLQDAKNFTRSYDQC 61
            P +  +P +  + ++  VH+   G     +    K+   G +WP M +  K++ RS   C
Sbjct: 1531 PRVWVVPTQLRRDLIFSVHDIPLGAHQRPEETYKKLRLLG-WWPGMQEHVKDYCRSCLFC 1590

Query: 62   QRFAPIPR----------QPPEPLTSSISPWPFTQWGIYLIGPLLQSKEQTKYAVVAVNY 121
                 IPR          + P PL S+ +PW   Q  I ++GP+  S+E  K+ ++  + 
Sbjct: 1591 -----IPRNLIGSELKVIESPWPLRST-APWSNLQ--IEVVGPVTISEEGHKHVLIVADP 1650

Query: 122  FTKWAKTEPLSTITEKKIADYIWKNIVCCFRIP 145
             T+W +  PL   T   +A  + +++   + +P
Sbjct: 1651 NTRWVEAFPLKPYTHTAVAQVLLQHVFARWGVP 1674

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158579.11.6e-5468.24uncharacterized protein LOC111025033 [Momordica charantia][more]
XP_022154103.11.4e-5366.89uncharacterized protein LOC111021439 [Momordica charantia][more]
XP_024036931.11.3e-5155.17uncharacterized protein LOC112096932 [Citrus clementina][more]
XP_020421233.18.3e-5153.55uncharacterized protein LOC109949580 [Prunus persica][more]
TQE13472.11.1e-5061.49hypothetical protein C1H46_001039 [Malus baccata][more]
Match NameE-valueIdentityDescription
Q9P2P19.3e-0524.18Protein NYNRIN OS=Homo sapiens OX=9606 GN=NYNRIN PE=2 SV=3[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 75..175
e-value: 3.3E-11
score: 44.8
NoneNo IPR availableGENE3D1.10.340.70coord: 2..64
e-value: 7.0E-12
score: 47.4
NoneNo IPR availablePANTHERPTHR24559:SF373REVERSE TRANSCRIPTASE DOMAIN, RIBONUCLEASE H-LIKE DOMAIN PROTEIN-RELATEDcoord: 19..148
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 19..148
IPR041588Integrase zinc-binding domainPFAMPF17921Integrase_H2C2coord: 8..65
e-value: 3.3E-9
score: 36.7
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 79..149

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027975.1Lag0027975.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity