Lag0027597 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027597
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pentatricopeptide repeat-containing protein
Location	chr8: 2380808 .. 2381248 (+)
RNA-Seq Expression	Lag0027597
Synteny	Lag0027597

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGCAGGGAGCAGTTGGTGAATCTGTTTGAGAAATGCAACAATAACAAAATTCTGGCGCAATTACAATGTCATACGCTCAAAATTGGCCTTACCCACGACAGTTTCTTCGCCACCAAACTCAGTGCTCTGCATGGGAAATACACCTCTCTCGTGCAAGCACACAAGGTGTTCGACGAAACGCCAAGCAAAACAGTACACCTCTGGAATGCTCTTCTCAGAAGCTACTGCAAAGAGAATCAATGGAGACGAACTTTACATCTCTTTCACGACATGGTATCCCAAGGAATTACAGAAGGGAAACCCGATAATTTCACAGTCCCTATCGTTTTGAAGGCCTGCGCAGGGTTGCAGGCACTTAAATTTGGAAAGACTGTTCATGGGTTTGTCAAGAAGAATGAGAAAACTGACCTCGACATGTTTGTTGGGCTGCGTTGA

mRNA sequence

ATGAAAAGCAGGGAGCAGTTGGTGAATCTGTTTGAGAAATGCAACAATAACAAAATTCTGGCGCAATTACAATGTCATACGCTCAAAATTGGCCTTACCCACGACAGTTTCTTCGCCACCAAACTCAGTGCTCTGCATGGGAAATACACCTCTCTCGTGCAAGCACACAAGGTGTTCGACGAAACGCCAAGCAAAACAGTACACCTCTGGAATGCTCTTCTCAGAAGCTACTGCAAAGAGAATCAATGGAGACGAACTTTACATCTCTTTCACGACATGGTATCCCAAGGAATTACAGAAGGGAAACCCGATAATTTCACAGTCCCTATCGTTTTGAAGGCCTGCGCAGGGTTGCAGGCACTTAAATTTGGAAAGACTGTTCATGGGTTTGTCAAGAAGAATGAGAAAACTGACCTCGACATGTTTGTTGGGCTGCGTTGA

Coding sequence (CDS)

ATGAAAAGCAGGGAGCAGTTGGTGAATCTGTTTGAGAAATGCAACAATAACAAAATTCTGGCGCAATTACAATGTCATACGCTCAAAATTGGCCTTACCCACGACAGTTTCTTCGCCACCAAACTCAGTGCTCTGCATGGGAAATACACCTCTCTCGTGCAAGCACACAAGGTGTTCGACGAAACGCCAAGCAAAACAGTACACCTCTGGAATGCTCTTCTCAGAAGCTACTGCAAAGAGAATCAATGGAGACGAACTTTACATCTCTTTCACGACATGGTATCCCAAGGAATTACAGAAGGGAAACCCGATAATTTCACAGTCCCTATCGTTTTGAAGGCCTGCGCAGGGTTGCAGGCACTTAAATTTGGAAAGACTGTTCATGGGTTTGTCAAGAAGAATGAGAAAACTGACCTCGACATGTTTGTTGGGCTGCGTTGA

Protein sequence

MKSREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFDETPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALKFGKTVHGFVKKNEKTDLDMFVGLR

Homology

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BLAST of Lag0027597 vs. NCBI nr
Match: XP_022982551.1 (putative pentatricopeptide repeat-containing protein At3g01580 isoform X1 [Cucurbita maxima])

HSP 1 Score: 265.0 bits (676), Expect = 3.8e-67
Identity = 124/144 (86.11%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MKSREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFD 60
           MKSR+QLV LFEKC  +KILA+LQC+TLKIGL HD+FFATKLSALHGKYTSLVQAHKVF+
Sbjct: 1   MKSRDQLVKLFEKCKCSKILAELQCYTLKIGLAHDTFFATKLSALHGKYTSLVQAHKVFE 60

Query: 61  ETPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQA 120
           ETPSKTVHLWNALLRSYCKE QW +TL+LF+DMVS GI+ GKPDNFT+PIVLKACAGLQA
Sbjct: 61  ETPSKTVHLWNALLRSYCKEKQWGQTLYLFNDMVSHGISNGKPDNFTIPIVLKACAGLQA 120

Query: 121 LKFGKTVHGFVKKNEKTDLDMFVG 145
           LKFGKTVHGF+KKNEKTDLDMFVG
Sbjct: 121 LKFGKTVHGFIKKNEKTDLDMFVG 144

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BLAST of Lag0027597 vs. NCBI nr
Match: XP_023526081.1 (putative pentatricopeptide repeat-containing protein At3g01580 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 264.2 bits (674), Expect = 6.5e-67
Identity = 123/144 (85.42%), Postives = 136/144 (94.44%), Query Frame = 0


Query: 1   MKSREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFD 60
           MKSR+QLV LFEKC  +KILA+LQC+TLKIGL HD+FFATKLSALHGKYTSLVQAHKVF+
Sbjct: 1   MKSRDQLVKLFEKCKCSKILAELQCYTLKIGLAHDTFFATKLSALHGKYTSLVQAHKVFE 60

Query: 61  ETPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQA 120
           ETPSKTVHLWNA+LRSYCKE QW +TL+LF+DMVS GI+ GKPDNFT+PIVLKACAGLQA
Sbjct: 61  ETPSKTVHLWNAILRSYCKEKQWGQTLYLFNDMVSHGISNGKPDNFTIPIVLKACAGLQA 120

Query: 121 LKFGKTVHGFVKKNEKTDLDMFVG 145
           LKFGKTVHGF+KKNEKTDLDMFVG
Sbjct: 121 LKFGKTVHGFIKKNEKTDLDMFVG 144

[Close]

BLAST of Lag0027597 vs. NCBI nr
Match: XP_008464928.1 (PREDICTED: putative pentatricopeptide repeat-containing protein At3g01580 [Cucumis melo] >KAA0057245.1 putative pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYK18716.1 putative pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 262.7 bits (670), Expect = 1.9e-66
Identity = 122/142 (85.92%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 3   SREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFDET 62
           SR+QLV LFEKCN++KILAQLQC TLK+GLTHDSFFATKLSALH KYTSLVQAHKVFDET
Sbjct: 5   SRDQLVRLFEKCNSSKILAQLQCLTLKLGLTHDSFFATKLSALHSKYTSLVQAHKVFDET 64

Query: 63  PSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALK 122
           P+KTVHLWNALLRSYCKENQW++TL+LFHDMVS GIT+GKPDN+T+PIVLKACAGLQAL+
Sbjct: 65  PTKTVHLWNALLRSYCKENQWKQTLYLFHDMVSYGITKGKPDNYTIPIVLKACAGLQALE 124

Query: 123 FGKTVHGFVKKNEKTDLDMFVG 145
           FGK VHGFV+K EKTD+DMFVG
Sbjct: 125 FGKMVHGFVEKMEKTDVDMFVG 146

[Close]

BLAST of Lag0027597 vs. NCBI nr
Match: XP_011652303.1 (putative pentatricopeptide repeat-containing protein At3g01580 [Cucumis sativus])

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-66
Identity = 122/142 (85.92%), Postives = 135/142 (95.07%), Query Frame = 0


Query: 3   SREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFDET 62
           SR+QLV LFEKCN++KILAQLQC TLK+GLTHDSFFATKLSALH KYTSL QAHKVFDET
Sbjct: 5   SRDQLVRLFEKCNSSKILAQLQCLTLKLGLTHDSFFATKLSALHSKYTSLEQAHKVFDET 64

Query: 63  PSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALK 122
           P+KTVHLWNALLRSYCKENQW++TLHLFHDMVS GIT+GKPDN+T+PIVLKACAGLQAL+
Sbjct: 65  PTKTVHLWNALLRSYCKENQWKQTLHLFHDMVSYGITKGKPDNYTIPIVLKACAGLQALE 124

Query: 123 FGKTVHGFVKKNEKTDLDMFVG 145
           FGK VHGFV+K EKTD+DMFVG
Sbjct: 125 FGKMVHGFVEKIEKTDVDMFVG 146

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BLAST of Lag0027597 vs. NCBI nr
Match: KAG7017786.1 (putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 261.5 bits (667), Expect = 4.2e-66
Identity = 122/144 (84.72%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MKSREQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFD 60
           MKSR+QLV LFEKC  +K LA+LQC+TLKIGL HD+FFATKLSALHGKYTSLVQAHKVF+
Sbjct: 1   MKSRDQLVKLFEKCKCSKNLAELQCYTLKIGLAHDTFFATKLSALHGKYTSLVQAHKVFE 60

Query: 61  ETPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQA 120
           ETPSKTVHLWNA+LRSYCKE QW +TL+LF+DMVS GI+ GKPDNFT+PIVLKACAGLQA
Sbjct: 61  ETPSKTVHLWNAILRSYCKEKQWGQTLYLFNDMVSHGISNGKPDNFTIPIVLKACAGLQA 120

Query: 121 LKFGKTVHGFVKKNEKTDLDMFVG 145
           LKFGKTVHGF+KKNEKTDLDMFVG
Sbjct: 121 LKFGKTVHGFIKKNEKTDLDMFVG 144

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BLAST of Lag0027597 vs. ExPASy Swiss-Prot
Match: Q9SS97 (Putative pentatricopeptide repeat-containing protein At3g01580 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E87 PE=3 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-17
Identity = 40/97 (41.24%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 48  KYTSLVQAHKVFDETPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFT 107
           K++S V A ++F E   ++++ WN LL+S  +E QW   L+ F  M      E KPDNFT
Sbjct: 6   KFSSSVDARQMFGEMTKRSLYQWNTLLKSLSREKQWEEVLYHFSHMFRD---EEKPDNFT 65

Query: 108 VPIVLKACAGLQALKFGKTVHGFVKKNEKTDLDMFVG 145
           +P+ LKAC  L+ + +G+ +HGFVKK+     D++VG
Sbjct: 66  LPVALKACGELREVNYGEMIHGFVKKDVTLGSDLYVG 99

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BLAST of Lag0027597 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.1e-14
Identity = 46/142 (32.39%), Postives = 76/142 (53.52%), Query Frame = 0


Query: 4   REQLVNLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALH--GKYTSLVQAHKVFDE 63
           R + ++L E+C + + L Q   H ++ G   D + A+KL A+     + SL  A KVFDE
Sbjct: 30  RSRHISLIERCVSLRQLKQTHGHMIRTGTFSDPYSASKLFAMAALSSFASLEYARKVFDE 89

Query: 64  TPSKTVHLWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQAL 123
            P      WN L+R+Y        ++  F DMVS+  ++  P+ +T P ++KA A + +L
Sbjct: 90  IPKPNSFAWNTLIRAYASGPDPVLSIWAFLDMVSE--SQCYPNKYTFPFLIKAAAEVSSL 149

Query: 124 KFGKTVHGFVKKNEKTDLDMFV 144
             G+++HG   K+     D+FV
Sbjct: 150 SLGQSLHGMAVKS-AVGSDVFV 168

[Close]

BLAST of Lag0027597 vs. ExPASy Swiss-Prot
Match: Q9LTV8 (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.1e-14
Identity = 43/135 (31.85%), Postives = 69/135 (51.11%), Query Frame = 0


Query: 9   NLFEKCNNNKILAQLQCHTLKIGLTHDSFFATKLSALHGKYTSLVQAHKVFDETPSKTVH 68
           +L +   +   L Q+    L +GL    F  TKL      +  +  A +VFD+ P   + 
Sbjct: 26  SLIDSATHKAQLKQIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIF 85

Query: 69  LWNALLRSYCKENQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALKFGKTVH 128
            WNA++R Y + N ++  L ++ +M    +    PD+FT P +LKAC+GL  L+ G+ VH
Sbjct: 86  PWNAIIRGYSRNNHFQDALLMYSNM---QLARVSPDSFTFPHLLKACSGLSHLQMGRFVH 145

Query: 129 GFVKKNEKTDLDMFV 144
             V +    D D+FV
Sbjct: 146 AQVFR-LGFDADVFV 156

[Close]

BLAST of Lag0027597 vs. ExPASy Swiss-Prot
Match: Q9LJJ1 (Putative pentatricopeptide repeat-containing protein At3g28640 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E79 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-13
Identity = 44/124 (35.48%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 22  QLQCHTLKIGL-THDSFFATKLSALHGKYTSLVQAHKVFDETPSKTVHLWNALLRSYCKE 81
           Q+ C  +K G+   DS   T +  ++ +   L+ A KVFDE P   V  W+ L+  Y + 
Sbjct: 137 QIHCWVVKNGVFLSDSHVQTGVLRIYVEDKLLLDARKVFDEIPQPDVVKWDVLMNGYVRC 196

Query: 82  NQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALKFGKTVHGFVKKNEKTDLD 141
                 L +F +M+ +G+   +PD F+V   L ACA + AL  GK +H FVKK    + D
Sbjct: 197 GLGSEGLEVFREMLVKGL---EPDEFSVTTALTACAQVGALAQGKWIHEFVKKKSWIESD 256

Query: 142 MFVG 145
           +FVG
Sbjct: 257 VFVG 257

[Close]

BLAST of Lag0027597 vs. ExPASy Swiss-Prot
Match: Q9LJI9 (Pentatricopeptide repeat-containing protein At3g28660 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E80 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 7.4e-13
Identity = 44/124 (35.48%), Postives = 64/124 (51.61%), Query Frame = 0


Query: 22  QLQCHTLKIGL-THDSFFATKLSALHGKYTSLVQAHKVFDETPSKTVHLWNALLRSYCKE 81
           Q+ C  +K G+   D    T +  ++ +   L  A KVFDE P   V  W+ L+  Y + 
Sbjct: 137 QIHCWVVKNGVFLSDGHVQTGVLRIYVEDKLLFDARKVFDEIPQPDVVKWDVLMNGYVRC 196

Query: 82  NQWRRTLHLFHDMVSQGITEGKPDNFTVPIVLKACAGLQALKFGKTVHGFVKKNEKTDLD 141
                 L +F +M+ +GI   +PD F+V   L ACA + AL  GK +H FVKK    + D
Sbjct: 197 GLGSEGLEVFKEMLVRGI---EPDEFSVTTALTACAQVGALAQGKWIHEFVKKKRWIESD 256

Query: 142 MFVG 145
           +FVG
Sbjct: 257 VFVG 257

The following BLAST results are available for this feature:

BLAST of Lag0027597 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982551.1	3.8e-67	86.11	putative pentatricopeptide repeat-containing protein At3g01580 isoform X1 [Cucur...	[more]
XP_023526081.1	6.5e-67	85.42	putative pentatricopeptide repeat-containing protein At3g01580 [Cucurbita pepo s...	[more]
XP_008464928.1	1.9e-66	85.92	PREDICTED: putative pentatricopeptide repeat-containing protein At3g01580 [Cucum...	[more]
XP_011652303.1	2.5e-66	85.92	putative pentatricopeptide repeat-containing protein At3g01580 [Cucumis sativus]	[more]
KAG7017786.1	4.2e-66	84.72	putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyros...	[more]

BLAST of Lag0027597 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS97	1.3e-17	41.24	Putative pentatricopeptide repeat-containing protein At3g01580 OS=Arabidopsis th...	[more]
O82380	5.1e-14	32.39	Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop...	[more]
Q9LTV8	5.1e-14	31.85	Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX...	[more]
Q9LJJ1	1.9e-13	35.48	Putative pentatricopeptide repeat-containing protein At3g28640 OS=Arabidopsis th...	[more]
Q9LJI9	7.4e-13	35.48	Pentatricopeptide repeat-containing protein At3g28660 OS=Arabidopsis thaliana OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011990	Tetratricopeptide-like helical domain superfamily	GENE3D	1.25.40.10	Tetratricopeptide repeat domain	coord: 3..138 e-value: 4.4E-14 score: 54.2
IPR002885	Pentatricopeptide repeat	TIGRFAM	TIGR00756	TIGR00756	coord: 69..98 e-value: 5.2E-8 score: 30.6
IPR002885	Pentatricopeptide repeat	PFAM	PF13041	PPR_2	coord: 66..116 e-value: 1.6E-11 score: 44.2
IPR002885	Pentatricopeptide repeat	PROSITE	PS51375	PPR	coord: 66..100 score: 10.961357
None	No IPR available	PANTHER	PTHR47924	PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN	coord: 21..144
None	No IPR available	PANTHER	PTHR47924:SF27	SUBFAMILY NOT NAMED	coord: 21..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027597.1	Lag0027597.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding