Lag0027585 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0027585 vs. NCBI nr
Match: XP_038905601.1 (GDSL esterase/lipase At5g14450-like isoform X1 [Benincasa hispida])

HSP 1 Score: 699.9 bits (1805), Expect = 1.2e-197
Identity = 323/375 (86.13%), Postives = 355/375 (94.67%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           MKMK+++   + V  ILIS  DSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF
Sbjct: 1   MKMKMKIQMALTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 120

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPFSLDIQIVQFRQFKNRTIDRYLEA +D+I++TLP+PE+FSKA+FTIDIGQNDLSAG
Sbjct: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEAVDDSIKNTLPIPEEFSKAIFTIDIGQNDLSAG 180

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTNAQF T IP+II EF+ A+E+LYKEGARAFWVHNTGPIGC+PV IRS+PNP+ GD
Sbjct: 181 FRKMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIPGD 240

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
           FDQNGCV+YQNDAALEFN++LKER++KLR  LSDAS++YVDVFAAK+KLIA AKEEGFME
Sbjct: 241 FDQNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGFME 300

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KGEICCGYHEG+NHVWCGNRKIINGSEVY GSCDDPSKFISWDGVHYTEAANRW+A+QI+
Sbjct: 301 KGEICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQIV 360

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQ+PITHAC
Sbjct: 361 KGSFSDPQIPITHAC 375

BLAST of Lag0027585 vs. NCBI nr
Match: XP_038905602.1 (GDSL esterase/lipase At5g14450-like isoform X2 [Benincasa hispida])

HSP 1 Score: 693.7 bits (1789), Expect = 8.6e-196
Identity = 322/375 (85.87%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           MKMK+++   + V  ILIS  DSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF
Sbjct: 1   MKMKMKIQMALTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 120

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPFSLDIQIVQFRQFKNRTIDRYLE  +D+I++TLP+PE+FSKA+FTIDIGQNDLSAG
Sbjct: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLE-VDDSIKNTLPIPEEFSKAIFTIDIGQNDLSAG 180

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTNAQF T IP+II EF+ A+E+LYKEGARAFWVHNTGPIGC+PV IRS+PNP+ GD
Sbjct: 181 FRKMTNAQFLTTIPNIIDEFSRAIEDLYKEGARAFWVHNTGPIGCIPVAIRSIPNPIPGD 240

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
           FDQNGCV+YQNDAALEFN++LKER++KLR  LSDAS++YVDVFAAK+KLIA AKEEGFME
Sbjct: 241 FDQNGCVRYQNDAALEFNKQLKERIIKLRTILSDASLVYVDVFAAKLKLIANAKEEGFME 300

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KGEICCGYHEG+NHVWCGNRKIINGSEVY GSCDDPSKFISWDGVHYTEAANRW+A+QI+
Sbjct: 301 KGEICCGYHEGNNHVWCGNRKIINGSEVYGGSCDDPSKFISWDGVHYTEAANRWIANQIV 360

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQ+PITHAC
Sbjct: 361 KGSFSDPQIPITHAC 374

BLAST of Lag0027585 vs. NCBI nr
Match: KAG7017790.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.0 bits (1743), Expect = 1.9e-190
Identity = 320/373 (85.79%), Postives = 348/373 (93.30%), Query Frame = 0

Query: 3   MKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHK 62
           MKI M+F   V  ILIS  DSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHK
Sbjct: 1   MKIPMAF-FTVASILISMADSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHK 60

Query: 63  TAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGV 122
           TAGRGCDGRLIIDFIAKHLELPYL+AYLNSIGANFRHGANFATGGSTIR QNESVFENGV
Sbjct: 61  TAGRGCDGRLIIDFIAKHLELPYLTAYLNSIGANFRHGANFATGGSTIRNQNESVFENGV 120

Query: 123 SPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFR 182
           SPFSLDIQ++QFRQFKNRTIDRY E T D IRSTLP+PEDFSKALFTIDIGQNDLSAGFR
Sbjct: 121 SPFSLDIQVIQFRQFKNRTIDRYREETNDFIRSTLPIPEDFSKALFTIDIGQNDLSAGFR 180

Query: 183 KMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFD 242
           KMTN QFR AIP+IIGEFAAA+EELY+EGARAFWVHNTGPIGC+PV IR+  +   GD D
Sbjct: 181 KMTNDQFRMAIPNIIGEFAAAIEELYREGARAFWVHNTGPIGCIPVAIRTTVH---GDLD 240

Query: 243 QNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKG 302
           QNGCVKYQNDAALEFNR+LKERV++LRAKLSDAS+++VDVFAAK++LIA AKEEGFME G
Sbjct: 241 QNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVHVDVFAAKLQLIANAKEEGFMEMG 300

Query: 303 EICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILKG 362
            ICCGYHEG++HVWCGN+KIINGS+VYAGSCDDPS+FISWDGVHYTEAANRW+A+QI+KG
Sbjct: 301 AICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQIIKG 360

Query: 363 SYSDPQVPITHAC 376
           S+SDPQVPITHAC
Sbjct: 361 SFSDPQVPITHAC 369

BLAST of Lag0027585 vs. NCBI nr
Match: XP_004141199.2 (GDSL esterase/lipase At5g14450 [Cucumis sativus] >KGN59717.1 hypothetical protein Csa_001756 [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 1.9e-190
Identity = 321/375 (85.60%), Postives = 347/375 (92.53%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           M MK++M F +  + ILIS  DSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TFF
Sbjct: 5   MNMKMKMVFSVASI-ILISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFF 64

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDFIAK LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 65  HKTAGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 124

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           G+SPFSLDIQ+VQFRQFKNRTIDRY+EA +D+IRSTLPVPE+FSKALFTIDIGQNDLSAG
Sbjct: 125 GISPFSLDIQVVQFRQFKNRTIDRYVEAIDDSIRSTLPVPEEFSKALFTIDIGQNDLSAG 184

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QFR AIPDII EFA AVE+LYKEGARAFWVHNTGPIGC+PV IRSV NP  GD
Sbjct: 185 FRKMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKEGD 244

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            D+NGCVK QNDAALEFNR+LKERVVKLRA L DAS++YVDV+AAK+KLIA AKEEGFME
Sbjct: 245 LDRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGFME 304

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KG ICCGYHEG NHVWCGNRK INGSEVYAGSC+DPSKFISWDGVHYTEAAN+W+A+QI+
Sbjct: 305 KGAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQIV 364

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQVPI HAC
Sbjct: 365 KGSFSDPQVPIMHAC 378

BLAST of Lag0027585 vs. NCBI nr
Match: XP_022982674.1 (GDSL esterase/lipase At5g14450-like [Cucurbita maxima])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 319/375 (85.07%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           M MKI M+F   V  ILIS  DSRAC FPAIYNFGDSNSDTGGISAAFYPTILPCGETFF
Sbjct: 1   MTMKIPMAF-FTVASILISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDF+AKHLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVFEN
Sbjct: 61  HKTAGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVFEN 120

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPFSLDIQ+VQFRQFKNRTIDRY E T D IRSTLPVPE+FS ALFTIDIGQNDLSAG
Sbjct: 121 GVSPFSLDIQVVQFRQFKNRTIDRYHEETNDFIRSTLPVPEEFSNALFTIDIGQNDLSAG 180

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QF+ AIP+IIGEF AA+EELY+EGARAFWVHNTGPIGC+PV IR+  +   GD
Sbjct: 181 FRKMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTTVH---GD 240

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            DQNGCVKYQNDAALEFNR+LKERV++LRAKLSDAS++YVDVFAAK++LIA AKEEGFME
Sbjct: 241 LDQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFME 300

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
            G ICCGYHEG++HVWCGN+KIINGS+VYAGSCDDPS+FISWDGVHYTEAANRW+A+QI+
Sbjct: 301 MGAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQII 360

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQVPITHAC
Sbjct: 361 KGSFSDPQVPITHAC 371

BLAST of Lag0027585 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.8e-125
Identity = 209/370 (56.49%), Postives = 273/370 (73.78%), Query Frame = 0

Query: 8   SFIIVVVWILISTVDSR-ACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGR 67
           S++++ ++ + ++V  +  C FPAIYNFGDSNSDTGGISAAF P   P G+ FFH+  GR
Sbjct: 18  SWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGR 77

Query: 68  GCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFS 127
             DGRL IDFIA+ L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++F+ G+SPFS
Sbjct: 78  DSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFS 137

Query: 128 LDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFRKMTN 187
           LD+QI QF QFK R+   + +      R  LP  E+F+KAL+T DIGQNDLS GFR M+ 
Sbjct: 138 LDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSV 197

Query: 188 AQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGC 247
            Q +  IPDI+   A+AV  +Y++G R FWVHNTGP GCLPV +  +  P  G  D++GC
Sbjct: 198 DQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGC 257

Query: 248 VKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICC 307
           VK QN+ A+EFNR+LKE V+ LR +L+ A+I YVDV+ AK ++++  K+ GF    ++CC
Sbjct: 258 VKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCC 317

Query: 308 GYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILKGSYSD 367
           GYHE  +H+WCGN+  +N +E+Y GSC +P   +SWDGVHYTEAAN+ VAD+ L G  +D
Sbjct: 318 GYHEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTD 377

Query: 368 PQVPITHACH 377
           P VPIT AC+
Sbjct: 378 PPVPITRACY 387

BLAST of Lag0027585 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-96
Identity = 171/358 (47.77%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 23  SRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGCDGRLIIDFIAKHLE 82
           S +C+FPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR  DGRLIIDFIA+ L 
Sbjct: 24  SPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSDGRLIIDFIAEELG 83

Query: 83  LPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTI 142
           LPYL+A+L+SIG+NF HGANFAT GST+R  N ++ ++GVSP SLD+Q+VQF  F  R+ 
Sbjct: 84  LPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDVQLVQFSDFITRS- 143

Query: 143 DRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFR-KMTNAQFRTAIPDIIGEFA 202
            + +       +  LP  E FS+AL+T DIGQNDL+AG +  MT+ Q +  IPD+  + +
Sbjct: 144 -QLIRNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQIKAYIPDVHDQLS 203

Query: 203 AAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCVKYQNDAALEFNREL 262
             + ++Y +G R FW+HNT P+GCLP  +   P P     D +GC   +N+ A  +N EL
Sbjct: 204 NVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVP-ASQIDNHGCAIPRNEIARYYNSEL 263

Query: 263 KERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCGY---HEGDNHVWCG 322
           K RV++LR +LS+A+  YVD+++ K+ LI  AK+ GF      CCG+   +  +  + CG
Sbjct: 264 KRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVACCGHGGKYNFNKLIKCG 323

Query: 323 NRKIINGSE-VYAGSCDDPSKFISWDGVHYTEAANRWVADQILKGSYSDPQVPITHAC 376
            + +I G E V A SC+D S  +SWDG+H+TE  N W+  QI  G++SDP +P+  AC
Sbjct: 324 AKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQINDGAFSDPPLPVKSAC 378

BLAST of Lag0027585 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.6e-96
Identity = 177/359 (49.30%), Postives = 238/359 (66.30%), Query Frame = 0

Query: 9   FIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGC 68
           F + ++  L  +  +  C FPAI+NFGDSNSDTGG+SAAF     P G +FF   AGR C
Sbjct: 9   FALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYC 68

Query: 69  DGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLD 128
           DGRL+IDFIA+ L LPYLSA+L+S+G+NF HGANFAT GS IR  N ++ ++G SPFSLD
Sbjct: 69  DGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLD 128

Query: 129 IQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG-FRKMTNA 188
           +Q VQF  F NR+  + + +     ++ LP  + FSKAL+T DIGQNDL+AG F   T  
Sbjct: 129 VQFVQFYNFHNRS--QTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVE 188

Query: 189 QFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCV 248
           Q  T +P+II +F  A++ +Y +G R FW+HNTGPIGCL   I   PN    DFD +GCV
Sbjct: 189 QVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNK-ASDFDSHGCV 248

Query: 249 KYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCG 308
              N  A +FN  LK+ V++LR+ LS+A+I YVDV++ K +L   A+  GF      CCG
Sbjct: 249 SPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCG 308

Query: 309 YHEGDNH---VWCGNRKIINGSEVYAGS-CDDPSKFISWDGVHYTEAANRWVADQILKG 363
           +    N+   + CG +KI+ G EVY G  CD+P K + WDGVH+T+AAN+++ D+I  G
Sbjct: 309 HGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG 364

BLAST of Lag0027585 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.0e-94
Identity = 182/357 (50.98%), Postives = 228/357 (63.87%), Query Frame = 0

Query: 26  CDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGCDGRLIIDFIAKHLELPY 85
           C FPA++NFGDSNSDTGG+S+ F     P G TFF   AGR CDGRL+IDFIA+ L L +
Sbjct: 38  CHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGMPAGRYCDGRLVIDFIAESLGLTH 97

Query: 86  LSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTIDRY 145
           LSAYLNSIG+NF  GANFAT GS+IRRQN S+F +G SP SLD+Q  +F QF NR+   Y
Sbjct: 98  LSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGFSPISLDVQFWEFEQFINRSQLVY 157

Query: 146 LEATEDAIRSTLPVPEDFSKALFTIDIGQNDL-SAGFRKMTNAQFRTAIPDIIGEFAAAV 205
                   R  LP  E FS+AL+T DIGQND+ S+ F   T  +    IPD++    + +
Sbjct: 158 -NNKGGIYREILPRAEYFSQALYTFDIGQNDITSSYFVNNTTEEVEAIIPDLMERLTSII 217

Query: 206 EELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCVKYQNDAALEFNRELKER 265
           + +Y  G R FW+HNTGP+GCLP  +   P+ L    D  GC    N  A  FN  LKE 
Sbjct: 218 QSVYSRGGRYFWIHNTGPLGCLPYALLHRPD-LAIPADGTGCSVTYNKVAQLFNLRLKET 277

Query: 266 VVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCGYHEG----DNHVWCGNR 325
           V  LR    DA+  YVDV+ AK KLI+ A + GF +    CCGY  G    D  V CG +
Sbjct: 278 VASLRKTHPDAAFTYVDVYTAKYKLISQANKLGFDDPLLTCCGYGGGRYNLDLSVGCGGK 337

Query: 326 KIINGSEVYAG-SCDDPSKFISWDGVHYTEAANRWVADQILKGSYSDPQVPITHACH 377
           K +NG+ V  G SC++PSK +SWDGVH+TEAAN++V DQI+ G+ SDP V +  ACH
Sbjct: 338 KQVNGTSVVVGKSCENPSKRVSWDGVHFTEAANKFVFDQIVAGALSDPPVALRQACH 392

BLAST of Lag0027585 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.1e-92
Identity = 174/377 (46.15%), Postives = 236/377 (62.60%), Query Frame = 0

Query: 5   IEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTA 64
           I +SF++ ++ +  +   S  CDFPAI+NFGDSNSDTGG +AAFYP   P GETFFH++ 
Sbjct: 12  ITLSFLLCMLSLAYA---SETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFFHRST 71

Query: 65  GRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESV-FENGVS 124
           GR  DGRLIIDFIA+   LPYLS YL+S+G+NF+HGA+FAT GSTI+     +    G S
Sbjct: 72  GRYSDGRLIIDFIAESFNLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAHGGFS 131

Query: 125 PFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFRK 184
           PF LD+Q  QFRQF  R+  +++  T       +P    F KAL+T DIGQNDL+ GF  
Sbjct: 132 PFYLDVQYSQFRQFIPRS--QFIRETGGIFAELVPEEYYFEKALYTFDIGQNDLTEGFLN 191

Query: 185 MTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQ 244
           +T  +    +PD++  F+A V+++Y  GAR FW+HNTGPIGCL   +   P     + D 
Sbjct: 192 LTVEEVNATVPDLVNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILTYFP---WAEKDS 251

Query: 245 NGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGE 304
            GC K  N+ A  FN +LKE V +LR  L  A+ ++VD+++ K  L +  ++ GF     
Sbjct: 252 AGCAKAYNEVAQHFNHKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGFEFPLI 311

Query: 305 ICCGYHEGDNH---VWCGNRKII-NGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQI 364
            CCGY    N      CG+     +G+++  GSC  PS  ++WDG HYTEAAN +  DQI
Sbjct: 312 TCCGYGGKYNFSVTAPCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEAANEYFFDQI 371

Query: 365 LKGSYSDPQVPITHACH 377
             G++SDP VP+  ACH
Sbjct: 372 STGAFSDPPVPLNMACH 380

BLAST of Lag0027585 vs. ExPASy TrEMBL
Match: A0A0A0LG79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G840430 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 9.0e-191
Identity = 321/375 (85.60%), Postives = 347/375 (92.53%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           M MK++M F +  + ILIS  DSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TFF
Sbjct: 5   MNMKMKMVFSVASI-ILISMADSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFF 64

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDFIAK LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 65  HKTAGRGCDGRLIIDFIAKQLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 124

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           G+SPFSLDIQ+VQFRQFKNRTIDRY+EA +D+IRSTLPVPE+FSKALFTIDIGQNDLSAG
Sbjct: 125 GISPFSLDIQVVQFRQFKNRTIDRYVEAIDDSIRSTLPVPEEFSKALFTIDIGQNDLSAG 184

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QFR AIPDII EFA AVE+LYKEGARAFWVHNTGPIGC+PV IRSV NP  GD
Sbjct: 185 FRKMTNDQFRKAIPDIINEFATAVEDLYKEGARAFWVHNTGPIGCIPVAIRSVSNPKEGD 244

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            D+NGCVK QNDAALEFNR+LKERVVKLRA L DAS++YVDV+AAK+KLIA AKEEGFME
Sbjct: 245 LDRNGCVKEQNDAALEFNRQLKERVVKLRANLLDASLVYVDVYAAKIKLIANAKEEGFME 304

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KG ICCGYHEG NHVWCGNRK INGSEVYAGSC+DPSKFISWDGVHYTEAAN+W+A+QI+
Sbjct: 305 KGAICCGYHEGLNHVWCGNRKTINGSEVYAGSCEDPSKFISWDGVHYTEAANQWIANQIV 364

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQVPI HAC
Sbjct: 365 KGSFSDPQVPIMHAC 378

BLAST of Lag0027585 vs. ExPASy TrEMBL
Match: A0A6J1J589 (GDSL esterase/lipase At5g14450-like OS=Cucurbita maxima OX=3661 GN=LOC111481479 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.9e-189
Identity = 319/375 (85.07%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           M MKI M+F   V  ILIS  DSRAC FPAIYNFGDSNSDTGGISAAFYPTILPCGETFF
Sbjct: 1   MTMKIPMAF-FTVASILISMADSRACGFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDF+AKHLELPYLSAYLNSIGANFRHGANFATGGSTIR QNESVFEN
Sbjct: 61  HKTAGRGCDGRLIIDFLAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRNQNESVFEN 120

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPFSLDIQ+VQFRQFKNRTIDRY E T D IRSTLPVPE+FS ALFTIDIGQNDLSAG
Sbjct: 121 GVSPFSLDIQVVQFRQFKNRTIDRYHEETNDFIRSTLPVPEEFSNALFTIDIGQNDLSAG 180

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QF+ AIP+IIGEF AA+EELY+EGARAFWVHNTGPIGC+PV IR+  +   GD
Sbjct: 181 FRKMTNYQFQMAIPNIIGEFDAAIEELYREGARAFWVHNTGPIGCIPVAIRTTVH---GD 240

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            DQNGCVKYQNDAALEFNR+LKERV++LRAKLSDAS++YVDVFAAK++LIA AKEEGFME
Sbjct: 241 LDQNGCVKYQNDAALEFNRQLKERVIQLRAKLSDASLVYVDVFAAKLQLIANAKEEGFME 300

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
            G ICCGYHEG++HVWCGN+KIINGS+VYAGSCDDPS+FISWDGVHYTEAANRW+A+QI+
Sbjct: 301 MGAICCGYHEGNDHVWCGNQKIINGSQVYAGSCDDPSRFISWDGVHYTEAANRWIANQII 360

Query: 361 KGSYSDPQVPITHAC 376
           KGS+SDPQVPITHAC
Sbjct: 361 KGSFSDPQVPITHAC 371

BLAST of Lag0027585 vs. ExPASy TrEMBL
Match: A0A1S3CN47 (GDSL esterase/lipase At5g14450-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.1e-187
Identity = 316/375 (84.27%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           MKMKI+++F +  + ILIS VDSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TFF
Sbjct: 3   MKMKIQLAFTVASI-ILISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFF 62

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDF AKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 63  HKTAGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 122

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPF LDIQ+VQFRQFKNRTIDRY+E  + +IRS+LPVPE+FSKALFTIDIGQNDLSAG
Sbjct: 123 GVSPFPLDIQVVQFRQFKNRTIDRYVE-VDGSIRSSLPVPEEFSKALFTIDIGQNDLSAG 182

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QF+ AIPDII EFA AVE+LYKEG RAFWVHNTGPIGC+PV IRS+  P  GD
Sbjct: 183 FRKMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGD 242

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            DQNGCVKYQN+AALEFNR+LKERVVKLRAKLS+A I+YVDV+AAK+KLIA AKEEGFME
Sbjct: 243 LDQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFME 302

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KG ICCGYHEG +HVWCGN+KIINGSEVYAGSC DPSKFISWDGVHYTEAAN+W+A+QI+
Sbjct: 303 KGAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIV 362

Query: 361 KGSYSDPQVPITHAC 376
            GS+SDPQVPI HAC
Sbjct: 363 DGSFSDPQVPIIHAC 375

BLAST of Lag0027585 vs. ExPASy TrEMBL
Match: A0A1S3CP81 (GDSL esterase/lipase At5g14450-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502686 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.7e-187
Identity = 316/375 (84.27%), Postives = 346/375 (92.27%), Query Frame = 0

Query: 1   MKMKIEMSFIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFF 60
           MKMKI+++F +  + ILIS VDSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TFF
Sbjct: 3   MKMKIQLAFTVASI-ILISMVDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFF 62

Query: 61  HKTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFEN 120
           HKTAGRGCDGRLIIDF AKHLELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFEN
Sbjct: 63  HKTAGRGCDGRLIIDFFAKHLELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFEN 122

Query: 121 GVSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG 180
           GVSPF LDIQ+VQFRQFKNRTIDRY+E  + +IRS+LPVPE+FSKALFTIDIGQNDLSAG
Sbjct: 123 GVSPFPLDIQVVQFRQFKNRTIDRYVE--DGSIRSSLPVPEEFSKALFTIDIGQNDLSAG 182

Query: 181 FRKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGD 240
           FRKMTN QF+ AIPDII EFA AVE+LYKEG RAFWVHNTGPIGC+PV IRS+  P  GD
Sbjct: 183 FRKMTNDQFQKAIPDIINEFATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGD 242

Query: 241 FDQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFME 300
            DQNGCVKYQN+AALEFNR+LKERVVKLRAKLS+A I+YVDV+AAK+KLIA AKEEGFME
Sbjct: 243 LDQNGCVKYQNNAALEFNRQLKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFME 302

Query: 301 KGEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQIL 360
           KG ICCGYHEG +HVWCGN+KIINGSEVYAGSC DPSKFISWDGVHYTEAAN+W+A+QI+
Sbjct: 303 KGAICCGYHEGVDHVWCGNKKIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIV 362

Query: 361 KGSYSDPQVPITHAC 376
            GS+SDPQVPI HAC
Sbjct: 363 DGSFSDPQVPIIHAC 374

BLAST of Lag0027585 vs. ExPASy TrEMBL
Match: A0A5A7UPV8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00290 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.4e-183
Identity = 306/355 (86.20%), Postives = 331/355 (93.24%), Query Frame = 0

Query: 21  VDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGCDGRLIIDFIAKH 80
           VDSRAC+FPAIYNFGDSNSDTGGISAAFYPTILPCG+TFFHKTAGRGCDGRLIIDF AKH
Sbjct: 2   VDSRACNFPAIYNFGDSNSDTGGISAAFYPTILPCGQTFFHKTAGRGCDGRLIIDFFAKH 61

Query: 81  LELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNR 140
           LELPYLSAYLNSIG NFRHGANFATGGSTIRRQNESVFENGVSPF LDIQ+VQFRQFKNR
Sbjct: 62  LELPYLSAYLNSIGTNFRHGANFATGGSTIRRQNESVFENGVSPFPLDIQVVQFRQFKNR 121

Query: 141 TIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFRKMTNAQFRTAIPDIIGEF 200
           TIDRY+E  + +IRS+LPVPE+FSKALFTIDIGQNDLSAGFRKMTN QF+ AIPDII EF
Sbjct: 122 TIDRYVE--DGSIRSSLPVPEEFSKALFTIDIGQNDLSAGFRKMTNDQFQKAIPDIINEF 181

Query: 201 AAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCVKYQNDAALEFNRE 260
           A AVE+LYKEG RAFWVHNTGPIGC+PV IRS+  P  GD DQNGCVKYQN+AALEFNR+
Sbjct: 182 ATAVEDLYKEGGRAFWVHNTGPIGCIPVAIRSISKPNPGDLDQNGCVKYQNNAALEFNRQ 241

Query: 261 LKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCGYHEGDNHVWCGNR 320
           LKERVVKLRAKLS+A I+YVDV+AAK+KLIA AKEEGFMEKG ICCGYHEG +HVWCGN+
Sbjct: 242 LKERVVKLRAKLSNAFIVYVDVYAAKIKLIANAKEEGFMEKGAICCGYHEGVDHVWCGNK 301

Query: 321 KIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILKGSYSDPQVPITHAC 376
           KIINGSEVYAGSC DPSKFISWDGVHYTEAAN+W+A+QI+ GS+SDPQVPI HAC
Sbjct: 302 KIINGSEVYAGSCKDPSKFISWDGVHYTEAANQWIANQIVDGSFSDPQVPIIHAC 354

BLAST of Lag0027585 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 449.9 bits (1156), Expect = 2.0e-126
Identity = 209/370 (56.49%), Postives = 273/370 (73.78%), Query Frame = 0

Query: 8   SFIIVVVWILISTVDSR-ACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGR 67
           S++++ ++ + ++V  +  C FPAIYNFGDSNSDTGGISAAF P   P G+ FFH+  GR
Sbjct: 18  SWLLLCLFAVTTSVSVQPTCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGR 77

Query: 68  GCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFS 127
             DGRL IDFIA+ L LPYLSAYLNS+G+NFRHGANFATGGSTIRRQNE++F+ G+SPFS
Sbjct: 78  DSDGRLTIDFIAERLGLPYLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFS 137

Query: 128 LDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFRKMTN 187
           LD+QI QF QFK R+   + +      R  LP  E+F+KAL+T DIGQNDLS GFR M+ 
Sbjct: 138 LDMQIAQFDQFKARSALLFTQIKSRYDREKLPRQEEFAKALYTFDIGQNDLSVGFRTMSV 197

Query: 188 AQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGC 247
            Q +  IPDI+   A+AV  +Y++G R FWVHNTGP GCLPV +  +  P  G  D++GC
Sbjct: 198 DQLKATIPDIVNHLASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGC 257

Query: 248 VKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICC 307
           VK QN+ A+EFNR+LKE V+ LR +L+ A+I YVDV+ AK ++++  K+ GF    ++CC
Sbjct: 258 VKAQNEMAMEFNRKLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFANPLKVCC 317

Query: 308 GYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILKGSYSD 367
           GYHE  +H+WCGN+  +N +E+Y GSC +P   +SWDGVHYTEAAN+ VAD+ L G  +D
Sbjct: 318 GYHEKYDHIWCGNKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTD 377

Query: 368 PQVPITHACH 377
           P VPIT AC+
Sbjct: 378 PPVPITRACY 387

BLAST of Lag0027585 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.1e-97
Identity = 171/358 (47.77%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 23  SRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGCDGRLIIDFIAKHLE 82
           S +C+FPAI+NFGDSNSDTGG+SA+F     P G+TFFH  +GR  DGRLIIDFIA+ L 
Sbjct: 24  SPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSDGRLIIDFIAEELG 83

Query: 83  LPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLDIQIVQFRQFKNRTI 142
           LPYL+A+L+SIG+NF HGANFAT GST+R  N ++ ++GVSP SLD+Q+VQF  F  R+ 
Sbjct: 84  LPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDVQLVQFSDFITRS- 143

Query: 143 DRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFR-KMTNAQFRTAIPDIIGEFA 202
            + +       +  LP  E FS+AL+T DIGQNDL+AG +  MT+ Q +  IPD+  + +
Sbjct: 144 -QLIRNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQIKAYIPDVHDQLS 203

Query: 203 AAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCVKYQNDAALEFNREL 262
             + ++Y +G R FW+HNT P+GCLP  +   P P     D +GC   +N+ A  +N EL
Sbjct: 204 NVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVP-ASQIDNHGCAIPRNEIARYYNSEL 263

Query: 263 KERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCGY---HEGDNHVWCG 322
           K RV++LR +LS+A+  YVD+++ K+ LI  AK+ GF      CCG+   +  +  + CG
Sbjct: 264 KRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVACCGHGGKYNFNKLIKCG 323

Query: 323 NRKIINGSE-VYAGSCDDPSKFISWDGVHYTEAANRWVADQILKGSYSDPQVPITHAC 376
            + +I G E V A SC+D S  +SWDG+H+TE  N W+  QI  G++SDP +P+  AC
Sbjct: 324 AKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQINDGAFSDPPLPVKSAC 378

BLAST of Lag0027585 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 353.2 bits (905), Expect = 2.6e-97
Identity = 177/359 (49.30%), Postives = 238/359 (66.30%), Query Frame = 0

Query: 9   FIIVVVWILISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFHKTAGRGC 68
           F + ++  L  +  +  C FPAI+NFGDSNSDTGG+SAAF     P G +FF   AGR C
Sbjct: 9   FALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPPHGSSFFGSPAGRYC 68

Query: 69  DGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENGVSPFSLD 128
           DGRL+IDFIA+ L LPYLSA+L+S+G+NF HGANFAT GS IR  N ++ ++G SPFSLD
Sbjct: 69  DGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLD 128

Query: 129 IQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAG-FRKMTNA 188
           +Q VQF  F NR+  + + +     ++ LP  + FSKAL+T DIGQNDL+AG F   T  
Sbjct: 129 VQFVQFYNFHNRS--QTVRSRGGVYKTMLPESDSFSKALYTFDIGQNDLTAGYFANKTVE 188

Query: 189 QFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNGCV 248
           Q  T +P+II +F  A++ +Y +G R FW+HNTGPIGCL   I   PN    DFD +GCV
Sbjct: 189 QVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYVIERFPNK-ASDFDSHGCV 248

Query: 249 KYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEICCG 308
              N  A +FN  LK+ V++LR+ LS+A+I YVDV++ K +L   A+  GF      CCG
Sbjct: 249 SPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCG 308

Query: 309 YHEGDNH---VWCGNRKIINGSEVYAGS-CDDPSKFISWDGVHYTEAANRWVADQILKG 363
           +    N+   + CG +KI+ G EVY G  CD+P K + WDGVH+T+AAN+++ D+I  G
Sbjct: 309 HGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDGVHFTQAANKFIFDKIAPG 364

BLAST of Lag0027585 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.5e-92
Identity = 178/374 (47.59%), Postives = 238/374 (63.64%), Query Frame = 0

Query: 4   KIEMSFIIVVVWIL--ISTVDSRACDFPAIYNFGDSNSDTGGISAAFYPTILPCGETFFH 63
           KI ++ I++++     +S + S +C+FPA++NFGDSNSDTG ISAA      P G  FF 
Sbjct: 5   KITLAIIVLLLGFTEKLSALSS-SCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFG 64

Query: 64  KTAGRGCDGRLIIDFIAKHLELPYLSAYLNSIGANFRHGANFATGGSTIRRQNESVFENG 123
           ++AGR  DGRLIIDFI ++L LPYL+ YL+S+GAN+RHGANFATGGS IR          
Sbjct: 65  RSAGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLAC----- 124

Query: 124 VSPFSLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGF 183
            SPF L  Q+ QF  FK RT+  Y +              DFSKAL+T+DIGQNDL+ GF
Sbjct: 125 FSPFHLGTQVSQFIHFKTRTLSLYNQT------------NDFSKALYTLDIGQNDLAIGF 184

Query: 184 RKMTNAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDF 243
           + MT  Q +  IP II  F  A++ LYKEGAR F +HNTGP GCLP  +++ P       
Sbjct: 185 QNMTEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPYLLKAFP---AIPR 244

Query: 244 DQNGCVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEK 303
           D  GC+K  N+ A+EFN++LK ++ +L+ +L  +   YVDV++AK  LI  AK  GF++ 
Sbjct: 245 DPYGCLKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFIDP 304

Query: 304 GEICCGYHEGDNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILK 363
            + CC    G   + CG    +NG+E+Y+ SC +   FISWDG+HYTE AN  VA++IL 
Sbjct: 305 FDYCCVGAIG-RGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILD 356

Query: 364 GSYSDPQVPITHAC 376
           GS SDP +P   AC
Sbjct: 365 GSISDPPLPTQKAC 356

BLAST of Lag0027585 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.7e-80
Identity = 162/366 (44.26%), Postives = 219/366 (59.84%), Query Frame = 0

Query: 12  VVVWILIS----TVDSRACDFPAIYNFGDSNSDTGG-ISAAFYPTILPCGETFFHKTAGR 71
           +V++ILIS    +  S    +PAI NFGDSNSDTG  ISA       P G+T+F+  +GR
Sbjct: 10  IVLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGR 69

Query: 72  GCDGRLIIDFIAKHLELPYLSAYLNSIG-ANFRHGANFATGGSTIRRQNESVFENGVSPF 131
            CDGRLI+DF+   ++LP+L+ YL+S+G  NF+ G NFA  GSTI   N +     VSPF
Sbjct: 70  YCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPT----SVSPF 129

Query: 132 SLDIQIVQFRQFKNRTIDRYLEATEDAIRSTLPVPEDFSKALFTIDIGQNDLSAGFRKMT 191
           S D+QI QF +FK+R I+  L  T       LP  + +SK L+ IDIGQND++  F   T
Sbjct: 130 SFDLQISQFIRFKSRAIE-LLSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKT 189

Query: 192 NAQFRTAIPDIIGEFAAAVEELYKEGARAFWVHNTGPIGCLPVTIRSVPNPLLGDFDQNG 251
             Q   +IP I+  F A ++ LY+EG R  W+HNTGP+GCL   I           D+ G
Sbjct: 190 LDQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDST-KLDEFG 249

Query: 252 CVKYQNDAALEFNRELKERVVKLRAKLSDASIIYVDVFAAKMKLIATAKEEGFMEKGEIC 311
           CV   N AA  FN +L     K +A+  DA++ YVD+F+ K  LIA     GF +    C
Sbjct: 250 CVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMAC 309

Query: 312 CGYHEG----DNHVWCGNRKIINGSEVYAGSCDDPSKFISWDGVHYTEAANRWVADQILK 368
           CG        D+ + CG  K+++G  V A +C+D S++I+WDG+HYTEAAN +V+ QIL 
Sbjct: 310 CGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILT 369