Lag0027396 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027396
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Aldo_ket_red domain-containing protein
Location	chr8: 131114 .. 133544 (+)
RNA-Seq Expression	Lag0027396
Synteny	Lag0027396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTGGGAAGGAAAGGAGGAGAGTGGTGATGGTGGAAGGGGATGGAAGGAAGAGAAATGGGTTTGAGTTTTAATTTTTTTGCCACATCAACAAAAAAAAATTATTTATTTATAAAATATTGCCATATCAACAGAAAAACAATAAAAATCAATTAAAAGGTGTCATATTATCTTTTTATTACTGTAGCCTATGAAAAATTAAGGGCAAAGATCATTTTGAAGTATTTTGAAAATTATGGTGGTCGACTTATTAAATTTTAAAAATTGGAGATTGAAGATAGTTATTTGTAAAAAAAATATCTATTCCTAGATATTAATATCTTTTAAGGTATTATACACGTTATTAAATATTTTGGGAGGAATACTTTAAAAAGTACTCTTGTAAGGACATCCAATTGGAGAAGTTTTTTTGTAACTTCATTTGGATGGGATTTATTACTTTTTTTTATACCATCAATGAAACTGTTTCCTATTAAAAAAAAAAAATACAAAAAAAATCTTGGAAGGATAAGTATAAACCCTATCTACTCTAAGTGAGACGAGGAAAATAAATCTTCTCTGATGTGGAATATCTATTTTATGCAGCTTAAAGCGAATGCAAACGGATTTCATTGATATTTACCAGATTCATTGGCCTGATCGGTTTGTATGTACTAACTTAATCTGTGAGATGTTATGGAAGTTAGAATTATATAGACTTTTTTCAGTGAATCATTGTCCAGACACCATTTTTGTGTTTTCTTATATTATTTTTGTTTTAGTTGTGAGAACAACACAATCATTTGTGTATGTTTTTCTTTTAAGGTATGTTCCAATGTTTGGAGAAACTGAATATAATCCATCCTGGCAGTATTGTTCAGTTACTATAGAAGAGCAACTGTATGCGCTCAGCAAGGCTGCTGATGCTGGTAAGGTAGGCGATTACAGTGCCATGTTCCTTTGTCACCCTCTCTGTATCGGATAGCATACCATGTTTTCAAGTAAGTTGGAATGTCTTGTGTTTTTAGATCAGATACATTGGCCTCAGCAATGAAACACCTTATGGTGTTATGAAATTTGCACAGACTGTTGAAAGATATCCCCACTTTCCGAAAGTTGTATCATTGCAGGTGCTTGAGCTCAATATGGTGCTTTTGATTCTATTTTATCGTATTTCTTGCTACTAAACGAAACATTTCTACTGTGAAGAATTCGTACAATTTGCTTTGTCGAACTTTTGATTCTGGCCTAGCTGAATGTTGCCATCACGAGAGGTAATAAATTTTTGGTTACATGACAAATTTAGTTCATGAATTTTCGCATTGTGTCTAATAGGTTATTGAATTGAACTTAAAAAGGTGTCGAATAAGTCTCTCAATTTTCAATTTTGTGTTTAATAAGTTGCTAAACTTTAAAAGTCTATTAAGTTATTGGACTTCTAATTTTGTGTCAAGTAGCTCTTTGACTTGTTTCAGATTTTCTAAAATTTATGGACTTATTAAACAGTTAAAAATGAGACCTATTAGACACTTTTTTTTTTTTGTTTTAGACATTTCTTTTAGTTTAAAGATCATATACAAAGCTTTTTAAAACTCATAGACTTATTAAATACACTATTGAATGTTTATTTATTTTCATTTTACGGATTATTCAACCTTTTCCAGAATCAGCTTATTAGCGTACAGTCCCCTGGCAATGGGCTTACTTTCTGGGAAGTACTTTTCTTCTGAAGGCGGTCCTGCAGATGCTCGACTTAATCTTTATAGAGGTCTCTATAAAATGCTTCCTCATGTCTTCTTTTCTGATATTAAATGCAAAGGAAATTGTTACAAATTCATTAGATGTGGACCTTTTATGTGAACTAAGGTGCTATTTTCATTCCAGGAAAATATTTAGAAGGGGAATCTAGATACAACCTAACCAATCACGTCATCAGAACAGCTACCATGGTAAGCAAATTCATGACTTTTAATTTTTAATTTTTTAGTACATGAAAACTCAAAAGATAATAGAGAGATGGATTGTAAAGCTTTTGCATTTTCTAATGTTGAAGAACGTCGGAAAAAACTTGTAGCATGATTGTTCCATTGTGACTATTACTTCAGACCCTAATGGCATTTGACGTGAATGCTTCAAAGTATATGGACTGTTTCATATTGTGCTTTCACTAACCTATTCTCTGTAATGTCTGTAGGAATATGTCAAGGTTGCTAAGAAATATAGGCTTCATCCAGTCTCTCTAGCAATTGGTTTGTTCTCCCTCTTCTTTTCCTCTCTGTGTATTGTTTCTTTCGAGGTGTGGGACATGTTCTGTAAAGTAGGTTGTAAGCCTTGCATCATTATGTGTTTTGAAATGTTGCAGAGTGGACTTAGGGTCTGTTTTGATGTTGCTTTTGGAGGGGTCAAAAGCAATCTTGAAAGAATTTTATTCGTTTTCGATGTTTGGTAG

mRNA sequence

ATGGGATTGGGAAGGAAAGGAGGAGAGTGCTTAAAGCGAATGCAAACGGATTTCATTGATATTTACCAGATTCATTGGCCTGATCGGTATGTTCCAATGTTTGGAGAAACTGAATATAATCCATCCTGGCAGTATTGTTCAGTTACTATAGAAGAGCAACTGTATGCGCTCAGCAAGGCTGCTGATGCTGGTAAGATCAGATACATTGGCCTCAGCAATGAAACACCTTATGGTGTTATGAAATTTGCACAGACTGTTGAAAGATATCCCCACTTTCCGAAAGTTGTATCATTGCAGAATTCGTACAATTTGCTTTGTCGAACTTTTGATTCTGGCCTAGCTGAATGTTGCCATCACGAGAGAATCAGCTTATTAGCGTACAGTCCCCTGGCAATGGGCTTACTTTCTGGGAAGTACTTTTCTTCTGAAGGCGGTCCTGCAGATGCTCGACTTAATCTTTATAGAGGAAAATATTTAGAAGGGGAATCTAGATACAACCTAACCAATCACGTCATCAGAACAGCTACCATGGAATATGTCAAGGTTGCTAAGAAATATAGGCTTCATCCAGTCTCTCTAGCAATTGGTTTGTTCTCCCTCTTCTTTTCCTCTCTGTGTATTGTTTCTTTCGAGGTGTGGGACATGTTCTGTAAAGTAGGTTGTAAGCCTTGCATCATTATGTGTTTTGAAATGTTGCAGAGTGGACTTAGGGTCTGTTTTGATGTTGCTTTTGGAGGGGTCAAAAGCAATCTTGAAAGAATTTTATTCGTTTTCGATGTTTGGTAG

Coding sequence (CDS)

ATGGGATTGGGAAGGAAAGGAGGAGAGTGCTTAAAGCGAATGCAAACGGATTTCATTGATATTTACCAGATTCATTGGCCTGATCGGTATGTTCCAATGTTTGGAGAAACTGAATATAATCCATCCTGGCAGTATTGTTCAGTTACTATAGAAGAGCAACTGTATGCGCTCAGCAAGGCTGCTGATGCTGGTAAGATCAGATACATTGGCCTCAGCAATGAAACACCTTATGGTGTTATGAAATTTGCACAGACTGTTGAAAGATATCCCCACTTTCCGAAAGTTGTATCATTGCAGAATTCGTACAATTTGCTTTGTCGAACTTTTGATTCTGGCCTAGCTGAATGTTGCCATCACGAGAGAATCAGCTTATTAGCGTACAGTCCCCTGGCAATGGGCTTACTTTCTGGGAAGTACTTTTCTTCTGAAGGCGGTCCTGCAGATGCTCGACTTAATCTTTATAGAGGAAAATATTTAGAAGGGGAATCTAGATACAACCTAACCAATCACGTCATCAGAACAGCTACCATGGAATATGTCAAGGTTGCTAAGAAATATAGGCTTCATCCAGTCTCTCTAGCAATTGGTTTGTTCTCCCTCTTCTTTTCCTCTCTGTGTATTGTTTCTTTCGAGGTGTGGGACATGTTCTGTAAAGTAGGTTGTAAGCCTTGCATCATTATGTGTTTTGAAATGTTGCAGAGTGGACTTAGGGTCTGTTTTGATGTTGCTTTTGGAGGGGTCAAAAGCAATCTTGAAAGAATTTTATTCGTTTTCGATGTTTGGTAG

Protein sequence

MGLGRKGGECLKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIGLSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPLAMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHPVSLAIGLFSLFFSSLCIVSFEVWDMFCKVGCKPCIIMCFEMLQSGLRVCFDVAFGGVKSNLERILFVFDVW

Homology

[Close]

BLAST of Lag0027396 vs. NCBI nr
Match: KAG7017894.1 (Protein tas, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.9 bits (1008), Expect = 2.2e-105
Identity = 190/206 (92.23%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQY SVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYSSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTATMEYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATMEYVKVAKKYGLHP 299

Query: 191 VSLAIGLFSLFFSSLCIVSFEVWDMF 217
           VSLAIGLFSLFF+SL IVSFE    F
Sbjct: 300 VSLAIGLFSLFFNSLYIVSFEALAAF 325

[Close]

BLAST of Lag0027396 vs. NCBI nr
Match: XP_023526182.1 (uncharacterized protein LOC111789740 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372.9 bits (956), Expect = 2.3e-99
Identity = 177/189 (93.65%), Postives = 183/189 (96.83%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTAT+EYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATVEYVKVAKKYGLHP 299

Query: 191 VSLAIGLFS 200
           VSLAI L S
Sbjct: 300 VSLAIDLTS 308

[Close]

BLAST of Lag0027396 vs. NCBI nr
Match: XP_023526183.1 (uncharacterized protein LOC111789740 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.3 bits (952), Expect = 6.8e-99
Identity = 175/185 (94.59%), Postives = 181/185 (97.84%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTAT+EYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATVEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. NCBI nr
Match: XP_023526181.1 (uncharacterized protein LOC111789740 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 371.3 bits (952), Expect = 6.8e-99
Identity = 175/185 (94.59%), Postives = 181/185 (97.84%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTAT+EYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATVEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. NCBI nr
Match: XP_022934446.1 (uncharacterized protein LOC111441623 [Cucurbita moschata])

HSP 1 Score: 369.0 bits (946), Expect = 3.4e-98
Identity = 175/185 (94.59%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQY SVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYSSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTATMEYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATMEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot
Match: P0A9T4 (Protein tas OS=Escherichia coli (strain K12) OX=83333 GN=tas PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.7e-32
Identity = 74/188 (39.36%), Postives = 117/188 (62.23%), Query Frame = 0


Query: 9   ECLKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRY 68
           + LKR+QTD++D+YQ+HWP R    FG+  Y+ +    +V++ + L AL++   AGKIRY
Sbjct: 116 DSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175

Query: 69  IGLSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYS 128
           IG+SNET +GVM++    +++   P++V++QN Y+LL R+F+ GLAE   +E + LLAYS
Sbjct: 176 IGVSNETAFGVMRYLHLADKH-DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS 235

Query: 129 PLAMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRY-NLTNHVIRTATMEYVKVAKKYR 188
            L  G L+GKY +    PA AR  L+        SR+   +    + A   YV +A+++ 
Sbjct: 236 CLGFGTLTGKYLNG-AKPAGARNTLF--------SRFTRYSGEQTQKAVAAYVDIARRHG 293

Query: 189 LHPVSLAI 196
           L P  +A+
Sbjct: 296 LDPAQMAL 293

[Close]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot
Match: P0A9T5 (Protein tas OS=Shigella flexneri OX=623 GN=tas PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.7e-32
Identity = 74/188 (39.36%), Postives = 117/188 (62.23%), Query Frame = 0


Query: 9   ECLKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRY 68
           + LKR+QTD++D+YQ+HWP R    FG+  Y+ +    +V++ + L AL++   AGKIRY
Sbjct: 116 DSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175

Query: 69  IGLSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYS 128
           IG+SNET +GVM++    +++   P++V++QN Y+LL R+F+ GLAE   +E + LLAYS
Sbjct: 176 IGVSNETAFGVMRYLHLADKH-DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS 235

Query: 129 PLAMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRY-NLTNHVIRTATMEYVKVAKKYR 188
            L  G L+GKY +    PA AR  L+        SR+   +    + A   YV +A+++ 
Sbjct: 236 CLGFGTLTGKYLNG-AKPAGARNTLF--------SRFTRYSGEQTQKAVAAYVDIARRHG 293

Query: 189 LHPVSLAI 196
           L P  +A+
Sbjct: 296 LDPAQMAL 293

[Close]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot
Match: P40690 (Aldo-keto reductase (Fragment) OS=Babesia bovis OX=5865 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 9.7e-24
Identity = 73/197 (37.06%), Postives = 105/197 (53.30%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYC-------SVTIEEQLYALSKAADA 70
           L R+ TD+ID+ Q+HWPDRYVPM    +++  +          SV +E+QL AL      
Sbjct: 39  LSRLNTDYIDLLQLHWPDRYVPMNANGDFHEVFHDTENMIDENSVPLEDQLDALQTLLTQ 98

Query: 71  GKIRYIGLSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRT-FDSGLAECCHHER- 130
           GKIR+ GLSNETP+G ++F + + +        S+Q  YNLLCR   + G  E C  +  
Sbjct: 99  GKIRHWGLSNETPWGTLRFYK-LAKQAGVAAPASVQLHYNLLCRNEVEKGFVELCRPQNT 158

Query: 131 -ISLLAYSPLAMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYV 190
            I++LAY+PLA G+L+GKY          RL L    Y+   SRY     +   A  +Y 
Sbjct: 159 GIAILAYAPLAGGILTGKYLEYMDPTTSGRL-LRFPSYM---SRYR--GSLAARAVKDYY 218

Query: 191 KVAKKYRLHPVSLAIGL 198
            +A  Y+ +P   A+ L
Sbjct: 219 NIAMSYK-YPNLCALAL 227

[Close]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot
Match: P80874 (Aldo-keto reductase YhdN OS=Bacillus subtilis (strain 168) OX=224308 GN=yhdN PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-12
Identity = 43/128 (33.59%), Postives = 63/128 (49.22%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKR+QTD+ID+YQ+HWPD  VP                 IEE    + +  DAGKIR IG
Sbjct: 111 LKRLQTDYIDLYQVHWPDPLVP-----------------IEETAEVMKELYDAGKIRAIG 170

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           +SN +        + ++ +     + ++Q  YNL  R  +  +       +I+ L Y  L
Sbjct: 171 VSNFS-------IEQMDTFRAVAPLHTIQPPYNLFEREMEESVLPYAKDNKITTLLYGSL 214

Query: 131 AMGLLSGK 139
             GLL+GK
Sbjct: 231 CRGLLTGK 214

[Close]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot
Match: P46336 (Aldo-keto reductase IolS OS=Bacillus subtilis (strain 168) OX=224308 GN=iolS PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 9.4e-11
Identity = 44/131 (33.59%), Postives = 62/131 (47.33%), Query Frame = 0


Query: 9   ECLKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRY 68
           E LKR+ TD+ID++ IH+PD + P                  +E + AL++   AGKIR 
Sbjct: 109 ESLKRLNTDYIDLFYIHFPDEHTPK-----------------DEAVNALNEMKKAGKIRS 168

Query: 69  IGLSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYS 128
           IG+SN +        + ++       V  LQ  YNLL R  +           IS + Y 
Sbjct: 169 IGVSNFS-------LEQLKEANKDGLVDVLQGEYNLLNREAEKTFFPYTKEHNISFIPYF 215

Query: 129 PLAMGLLSGKY 140
           PL  GLL+GKY
Sbjct: 229 PLVSGLLAGKY 215

[Close]

BLAST of Lag0027396 vs. ExPASy TrEMBL
Match: A0A6J1F1U5 (uncharacterized protein LOC111441623 OS=Cucurbita moschata OX=3662 GN=LOC111441623 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.6e-98
Identity = 175/185 (94.59%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQY SVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYSSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARLNLYRG+YLEGESRYNLTN++IRTATMEYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLNLYRGRYLEGESRYNLTNNIIRTATMEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. ExPASy TrEMBL
Match: A0A6J1J005 (uncharacterized protein LOC111482217 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482217 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.1e-98
Identity = 174/185 (94.05%), Postives = 179/185 (96.76%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGP DARL LYRG+YLEGESRYNLTN++IRTATMEYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPEDARLKLYRGRYLEGESRYNLTNNIIRTATMEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. ExPASy TrEMBL
Match: A0A6J1J8D8 (uncharacterized protein LOC111482217 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482217 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 2.1e-98
Identity = 174/185 (94.05%), Postives = 179/185 (96.76%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG
Sbjct: 120 LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA T E+YPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHTGEKYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGP DARL LYRG+YLEGESRYNLTN++IRTATMEYVKVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPEDARLKLYRGRYLEGESRYNLTNNIIRTATMEYVKVAKKYGLHP 299

Query: 191 VSLAI 196
           VSLAI
Sbjct: 300 VSLAI 304

[Close]

BLAST of Lag0027396 vs. ExPASy TrEMBL
Match: A0A0A0LAL1 (Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G257070 PE=4 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 2.0e-96
Identity = 170/185 (91.89%), Postives = 178/185 (96.22%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKR+QTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQL+ALSKAADAGKIRYIG
Sbjct: 120 LKRLQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLFALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA   ERYPH PKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHINERYPHIPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARL+L+RGKYLEGESRYNLTN V+RTATMEY+KVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLHLFRGKYLEGESRYNLTNTVVRTATMEYIKVAKKYGLHP 299

Query: 191 VSLAI 196
           VS+AI
Sbjct: 300 VSMAI 304

[Close]

BLAST of Lag0027396 vs. ExPASy TrEMBL
Match: A0A5A7U2N4 (Protein tas-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G00900 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.4e-94
Identity = 168/185 (90.81%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKR+QTDFIDIYQIHWPDRYVPMFGETEYNPSWQY SVTIEEQL+ALSKAADAGKIRYIG
Sbjct: 120 LKRLQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYSSVTIEEQLFALSKAADAGKIRYIG 179

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 130
           LSNETPYGVMKFA   ERYPH PKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL
Sbjct: 180 LSNETPYGVMKFAHIGERYPHVPKVVSLQNSYNLLCRTFDSGLAECCHHERISLLAYSPL 239

Query: 131 AMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKYRLHP 190
           AMG+LSGKYFS EGGPADARL+L+RGKYLEGESRYNL N VIRTATM+Y+KVAKKY LHP
Sbjct: 240 AMGILSGKYFSPEGGPADARLHLFRGKYLEGESRYNLNNTVIRTATMDYIKVAKKYGLHP 299

Query: 191 VSLAI 196
           VS+AI
Sbjct: 300 VSMAI 304

[Close]

BLAST of Lag0027396 vs. TAIR 10
Match: AT1G04420.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-40
Identity = 94/190 (49.47%), Postives = 122/190 (64.21%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKR+ TD+ID+ QIHWPDRYVP+FG+  Y  S    SV   EQL A       GK+RYIG
Sbjct: 174 LKRLGTDYIDLLQIHWPDRYVPLFGDFYYETSKWRPSVPFAEQLRAFQDLIVEGKVRYIG 233

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCR-TFDSGLAECCHHE--RISLLAY 130
           +SNET YGV +F  T  +    PK+VS+QN Y+LL R  ++  L E CH +   + LLAY
Sbjct: 234 VSNETSYGVTEFVNTA-KLEGLPKIVSIQNGYSLLVRCRYEVDLVEVCHPKNCNVGLLAY 293

Query: 131 SPLAMGLLSGKYFSS-EGGPADARLNLYRGKYLEGESRYNLTNHVIRTATMEYVKVAKKY 190
           SPL  G LSGKY ++ +    +ARLNL+ G Y+E   RY     + + AT++YV+VAKKY
Sbjct: 294 SPLGGGSLSGKYLATDQEATKNARLNLFPG-YME---RYK--GSLAKEATIQYVEVAKKY 353

Query: 191 RLHPVSLAIG 197
            L PV LA+G
Sbjct: 354 GLTPVELALG 356

[Close]

BLAST of Lag0027396 vs. TAIR 10
Match: AT1G06690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-10
Identity = 43/153 (28.10%), Postives = 74/153 (48.37%), Query Frame = 0


Query: 9   ECLKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRY 68
           + L R++   +D+YQ+HWP     ++G   Y              L  L  A + G ++ 
Sbjct: 163 DSLSRLELSSVDLYQLHWPG----LWGNEGY--------------LDGLGDAVEQGLVKA 222

Query: 69  IGLSNETPYGVMKFAQTVERY-PHFPKVVSLQNSYNLLCRTFD-SGLAECCHHERISLLA 128
           +G+SN   Y   +     ER       + S Q +Y+L+ R  + +G+   C    ++L+A
Sbjct: 223 VGVSN---YSEKRLRDAYERLKKRGIPLASNQVNYSLIYRAPEQTGVKAACDELGVTLIA 282

Query: 129 YSPLAMGLLSGKYFSSEGGPADARLNLYRGKYL 160
           YSP+A G L+GKY + E  P+  R  +Y  ++L
Sbjct: 283 YSPIAQGALTGKY-TPENPPSGPRGRIYTREFL 293

[Close]

BLAST of Lag0027396 vs. TAIR 10
Match: AT1G04690.1 (potassium channel beta subunit 1 )

HSP 1 Score: 51.6 bits (122), Expect = 1.1e-06
Identity = 45/164 (27.44%), Postives = 69/164 (42.07%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           LKR+  D++D+   H PD   P                 IEE + A++   D G   Y G
Sbjct: 108 LKRLDMDYVDVLYCHRPDASTP-----------------IEETVRAMNYVIDKGWAFYWG 167

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCR-TFDSGLAECCHHERISLLAYSP 130
            S  +   + +     +R      +V  Q  YN+  R   ++       +  I L  +SP
Sbjct: 168 TSEWSAQQITEAWGAADRLDLVGPIVE-QPEYNMFARHKVETEFLPLYTNHGIGLTTWSP 227

Query: 131 LAMGLLSGKYFSSEGGPADARLNLYRGKYLEGESRYNLTNHVIR 174
           LA G+L+GKY +    P+D+R  L   K L   S   L + V+R
Sbjct: 228 LASGVLTGKY-NKGAIPSDSRFALENYKNLANRS---LVDDVLR 249

[Close]

BLAST of Lag0027396 vs. TAIR 10
Match: AT5G53580.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 5.5e-06
Identity = 41/146 (28.08%), Postives = 66/146 (45.21%), Query Frame = 0


Query: 11  LKRMQTDFIDIYQIHWPDRYVPMFGETEYNPSWQYCSVTIEEQLYALSKAADAGKIRYIG 70
           L R+Q D + I Q+HW            Y P  +        Q+Y      + G +R +G
Sbjct: 150 LDRLQIDQLGIGQLHW--------STASYAPLQELVLWDGLVQMY------EKGLVRAVG 209

Query: 71  LSNETPYGVMKFAQTVERYPHFPKVVSLQNSYNLLCRTFDS-GLAECCHHERISLLAYSP 130
           +SN  P  ++K    ++       + S Q  ++LL    +   +   C    I L++YSP
Sbjct: 210 VSNYGPQQLVKIHDYLK--TRGVPLCSAQVQFSLLSMGKEQLEIKSICDELGIRLISYSP 269

Query: 131 LAMGLLSGKYFSSEGGPADARLNLYR 156
           L +G+L+GKY SS+  P   R  L+R
Sbjct: 270 LGLGMLTGKYSSSK-LPTGPRSLLFR 278

The following BLAST results are available for this feature:

BLAST of Lag0027396 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017894.1	2.2e-105	92.23	Protein tas, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023526182.1	2.3e-99	93.65	uncharacterized protein LOC111789740 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023526183.1	6.8e-99	94.59	uncharacterized protein LOC111789740 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023526181.1	6.8e-99	94.59	uncharacterized protein LOC111789740 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022934446.1	3.4e-98	94.59	uncharacterized protein LOC111441623 [Cucurbita moschata]	[more]

BLAST of Lag0027396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0A9T4	5.7e-32	39.36	Protein tas OS=Escherichia coli (strain K12) OX=83333 GN=tas PE=1 SV=1	[more]
P0A9T5	5.7e-32	39.36	Protein tas OS=Shigella flexneri OX=623 GN=tas PE=3 SV=1	[more]
P40690	9.7e-24	37.06	Aldo-keto reductase (Fragment) OS=Babesia bovis OX=5865 PE=2 SV=1	[more]
P80874	1.7e-12	33.59	Aldo-keto reductase YhdN OS=Bacillus subtilis (strain 168) OX=224308 GN=yhdN PE=...	[more]
P46336	9.4e-11	33.59	Aldo-keto reductase IolS OS=Bacillus subtilis (strain 168) OX=224308 GN=iolS PE=...	[more]

BLAST of Lag0027396 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1U5	1.6e-98	94.59	uncharacterized protein LOC111441623 OS=Cucurbita moschata OX=3662 GN=LOC1114416...	[more]
A0A6J1J005	2.1e-98	94.05	uncharacterized protein LOC111482217 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1J8D8	2.1e-98	94.05	uncharacterized protein LOC111482217 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0LAL1	2.0e-96	91.89	Aldo_ket_red domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G25707...	[more]
A0A5A7U2N4	2.4e-94	90.81	Protein tas-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold600G009...	[more]

BLAST of Lag0027396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G04420.1	2.6e-40	49.47	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT1G06690.1	2.2e-10	28.10	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT1G04690.1	1.1e-06	27.44	potassium channel beta subunit 1	[more]
AT5G53580.1	5.5e-06	28.08	NAD(P)-linked oxidoreductase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020471	Aldo-keto reductase	PRINTS	PR00069	ALDKETRDTASE	coord: 57..74 score: 37.65 coord: 9..27 score: 43.34
IPR036812	NADP-dependent oxidoreductase domain superfamily	GENE3D	3.20.20.100		coord: 8..198 e-value: 1.2E-40 score: 141.4
IPR036812	NADP-dependent oxidoreductase domain superfamily	SUPERFAMILY	51430	NAD(P)-linked oxidoreductase	coord: 9..195
IPR023210	NADP-dependent oxidoreductase domain	PFAM	PF00248	Aldo_ket_red	coord: 9..195 e-value: 2.3E-31 score: 109.1
None	No IPR available	PANTHER	PTHR43147	PROTEIN TAS	coord: 10..197
None	No IPR available	PANTHER	PTHR43147:SF2	PROTEIN TAS	coord: 10..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027396.1	Lag0027396.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0047834	D-threo-aldose 1-dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity