Lag0027235 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027235
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	AdoMet-dependent rRNA methyltransferase spb1
Location	chr10: 46051794 .. 46055610 (-)
RNA-Seq Expression	Lag0027235
Synteny	Lag0027235

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGCTTCAAGAGATAAAAGGGTTTAAGCACCATTCTATTTGACTATTTGTCTTTCTGATTCATTATCTTTTTCCATGTTTTATTAACTCGGTCTCGTTTGCAGGATATATACTATCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGTTTGTTTTTATATTGGGTTCCTCTCTCTTAAAATGTTTAGAATGTCCATCTCTGAGATGGTCCAGATGAATGGTAATAAATATTCCTTTTTCAAGAATACTCTTTCCCTTTGTTTATTAGTAGTTCTGTCACCTGGAGATGCCATTTTTTTCCTCGTGAACAATGCTGCAATATTTTGGATTAACCTTCCATAATAGAATTGTTCTCCAGAATCCTCCAAAATCTGTGTTTTATTTGGCTCTGCAATCTTCCTGTTTTATCGTGTACAAGTCATTTGAAATACTTCTAAGTACATTGTCTACAACAAAATTTCAAATGCAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTAATCTCTTTTTGAGTGTCTTCTTTTTTTTTTTTTTTTTTTGCAATCTGTGTATCGAACTAGTGGACAATGCTTTATGGGGAAATACAAACTTTCATGTAAAACACAACTAATTCTATCAAGCATTCTTCAAGTGAATGATAAGCTTCCTTTTTTTTTTTTTGGTGGGAAAAGCATGCCTATATATGGGGTGCAAGCCTCTTATAAACGCTTGATTTAATCATTCTTATAAAAGCTTGTTTTAAATTTTCATTATCTCATTTTTCAGAACAATATATATTTTTAATCTTTATTTTGCATTAAACTTATAGGAGTACTGTGCTTCAGCATACTCCACGGTTTGAAAAACACTGAACATAATGAGGGCTGGATAAATGCAATGTATTATGTGTTGCTGCAACTGTCGCTTAAAAATATGATTTATTTTGGTTAATTATTTCCAAGTCTCATTTTCTCACATACTAATCATATGTAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTTCTGATTCAAAGTAAGTTATGGTAGATTATCATATAAAAATGTTACTTTTTCCTTCATATCCTTCTCTCTATTTCCTCCTTGTGACCTCCCTCTTTCACTGATTCTCTAGCTTAATTTGCAGGGATGGTGATCTTCCTCTTATCGTTGCTATCGATTTGCAGCCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCAGAGTGTACCATGTACACTGCTTTTAACATTTGTTTAAAATTTTTTCGAAGTATTAAATATTTTATTAACTAAATGAAGACATATCCAAATTGGCAGATCCTATGCAACAGATTTGGTCAATGTTGTTTTAAAATTTCTATTTGTGATAATTTTTCTTATATTTTTCTCAATATTGGACATTTTTTCTCTTTTGAATGGATACTCACTTTGTGTTAGAAGTCTTTTATTTTATTCAACAATTTGATCAAATATTAAATTGTCATATATCTTTTTGTGCATGGCTTTAGCTTATCAAATATTTACCTTTTCCAGGTCATTAAGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCACCTGATGGTAGATACATGTGTTTTTGAATTCTCTGCTGAGATTCCACCTGATGACATTTTTCTTACTGATTATGTGCTTGTTTGTAGTTACTGGTCTTCATGACATGGATGAATTTGTTCAGTCCCAGCTCATACTAGCTGTAAGTGCTGTCTTTTTCTTCAGAGACTGATTAAATTTCTCATGGTGATGCTGATGCATAGTTTTAAGATATTTTGAAAATGCATGAGGTATATGCACGTTGACAGTAATGATGTTGCTTCAGCTCTCGTTTACAGTAACCAACAAGCAGCATTGATGAGTGTCAAATTGTTTGAACCAATGAATAGAAAATAACGTCCCTTTAGGTCAAATGTGATTGCTGTATTATGAAATTATATTGAAGCATTTCAAGAGTTTTTCTATGTTATGTTGCCTGCACTGGTTGAACCTTTTCTTCTATGGACTGAATTTTGTTGCTTCCATCACTGTCCTCTGCTGCCTCTTTTTTCCTCCCTCTCTCTCTTCCTTGAGCTAGTTTTCAATATAGTTGGACATACATAAGGTTCTCATAGCTGAGCTACACTTTTTTCTTCCCTTTTCTTCGCAGGGTTTGACAATAGTTACTCATGTACTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGGTATATATTAAATGGTTGTTACAATACCCAATCAAAGAAATATGTAGTATGCTCTAGTAACAACGAAATTTATGGTGTTCTCACTACCTGCAATATGAAACATTTTCTTTTTGTTTGTCAGTCAAGATGAAAGGTTTGCTGTGAGCTCTTCTGTTCCTTGTAATTATTGCGTTGATGTGTAGATTCTTTTTGGATATGTTTAGCACGAGTAAAACTTGTCAAGTATTATCATGAATTACATTAGGACCATCAAAGATAGTTGTTTGTTGCTTCATACATGAAGGTGAAGCCGTCAATGATGTTTTACATTTTCGAGTTTTTTGTTGCAGCTGAAGTTATTTTTCCCTGTTGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGGTAACCTTTTGGTCTCCCACTTATTTTATTGGTAGCTCTATAGGTCTATCTTTCGGTTATAATTGAACTTGTTTCCAGAAAATGATACCCTCCTAATTGAAAATCGCCTTTTTGTGTCTTTATAGCGTCTGGTTCATAGGATTCTTGCGCATGAATTTTCAGATACCCATTGTACTTAGATTCCTTCAATCAACTTTAGATTGTTATCGTTTAATACTAGGTGGAACTGGTGTCCACCCTACAGCAAATTGTGGAGTTAGCTCATTTTCGTGGTGACATTTGTAATATAAAAAAAACAAGTAAAAGAGGACAAAATAGTCAAAGGTAATTCATTCCAGGGCTTTCTTTTAGTCATCTGGGTGAACCCTCGGTAAAAACTTTTAAACAAAGTTTAGATTGTGATTTAAATTTATGTTGATGTAGAAGCTCATACGTGGACTGGGTGTGAATGCAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTGCATCGCCTGCTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGGTAAGCTCAATGTACTGCCTTGATAATGAGTTTACATGTCGATGGACGTATCCTAAGATCGAGCATGTTCTTATCCTTCTTTTAAAGATTAATTGTTTTACAGCTGGCTATGACCAAGCTGTCTTCTTGTCTTTAGGCTTTTTAAAATTCGTGTCTCTGATTTTGATGTGGTCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTGCATTGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGAAGGCCCCAATAAAGTTTACATCCCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGCGGGAGCTTATCGGAGCTTAGATCCTGTGCAACCCCCTATCGCCCCTCCTTACAAGAGGGCGCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

mRNA sequence

ATGGGCAAAGCTTCAAGAGATAAAAGGGATATATACTATCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTTCTGATTCAAAGGATGGTGATCTTCCTCTTATCGTTGCTATCGATTTGCAGCCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAAGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCACCTGATGTTACTGGTCTTCATGACATGGATGAATTTGTTCAGTCCCAGCTCATACTAGCTGGTTTGACAATAGTTACTCATGTACTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTTGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTGCATCGCCTGCTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGCCCCAATAAAGTTTACATCCCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGCGGGAGCTTATCGGAGCTTAGATCCTGTGCAACCCCCTATCGCCCCTCCTTACAAGAGGGCGCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Coding sequence (CDS)

ATGGGCAAAGCTTCAAGAGATAAAAGGGATATATACTATCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGCGAGTGGTGGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTTAGTAGGAAATTGTATCTCCCAGCAAAGCTTTCTTCTGATTCAAAGGATGGTGATCTTCCTCTTATCGTTGCTATCGATTTGCAGCCGATGGCTCCAATTGAAGGTGTTATTCAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAAGCATTTTGATGGGTGTAAGGCTGACCTGGTGGTTTGTGATGGTGCACCTGATGTTACTGGTCTTCATGACATGGATGAATTTGTTCAGTCCCAGCTCATACTAGCTGGTTTGACAATAGTTACTCATGTACTTAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACAAGCCTTTTGTATTGTCAGCTGAAGTTATTTTTCCCTGTTGTGACTTTTGCAAAACCAAAAAGCAGTCGCAATTCCAGTATAGAGGCATTTGCAGTTTGTGAGAATTATTCTCCTCCCGAAGGTTTCAACCCAAAAGATCTGCATCGCCTGCTAGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGCCCCAATAAAGTTTACATCCCGTTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCCGCGGGAGCTTATCGGAGCTTAGATCCTGTGCAACCCCCTATCGCCCCTCCTTACAAGAGGGCGCTTGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Protein sequence

MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSLDS

Homology

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BLAST of Lag0027235 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 627.9 bits (1618), Expect = 4.9e-176
Identity = 309/316 (97.78%), Postives = 312/316 (98.73%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKL+LPAKLS DSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLHLPAKLSPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YR LDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Lag0027235 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 627.1 bits (1616), Expect = 8.3e-176
Identity = 309/316 (97.78%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS DSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 201 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 260

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 261 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 320

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 321 ASSQGIRELEKLSSDS 336

[Close]

BLAST of Lag0027235 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 627.1 bits (1616), Expect = 8.3e-176
Identity = 309/316 (97.78%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS DSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Lag0027235 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 307/316 (97.15%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS D KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Lag0027235 vs. NCBI nr
Match: XP_022983316.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima] >XP_022983317.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 620.9 bits (1600), Expect = 5.9e-174
Identity = 307/316 (97.15%), Postives = 309/316 (97.78%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS DSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 359.0 bits (920), Expect = 5.5e-98
Identity = 176/300 (58.67%), Postives = 217/300 (72.33%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG+ S+DKRD+YYR AKE GWRARSAFKLLQ+D+EF +F+GV R VDLCAAPGSWSQVLS
Sbjct: 1   MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           +K+            G    +VA+DLQ MAP+ GV+Q+QGDIT   TA+ +I+HF GC A
Sbjct: 61  QKI-------GGQGSGH---VVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHD+DE++Q+QL+LA L I THVL+ GG F+AKIFRG+D +LLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           ++FF  V  AKP+SSRNSSIEAFAVC+ Y PPEGF P     LL+    P          
Sbjct: 181 QVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ------ 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
             L+GP ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 241 --LDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

[Close]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.1e-89
Identity = 169/301 (56.15%), Postives = 219/301 (72.76%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG++S+DKRD YYR AKE+GWRARSAFKLLQ++E+FN+FEG KRVVDLCAAPGSWSQVLS
Sbjct: 1   MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLY--LPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGC 120
           R+L   +   +++D K    P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF   
Sbjct: 61  RELLKNIDTSIAADEK----PMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNE 120

Query: 121 KADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC 180
            ADLVV DGAPDVTGLHD+DE++Q+Q++LA   +   VL+ GGKF+AKIFRG+D SLLY 
Sbjct: 121 PADLVVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYS 180

Query: 181 QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
           QL+L F  V+ AKP+SSR SSIE+F VCE+++PP  F P DL + L  V  P+   ++  
Sbjct: 181 QLRLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI-- 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEM 300
                        PF+ACGDL GYD+D +YP+      A  +LD +QPP APPYKRA+E+
Sbjct: 241 ------------APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIEL 279

[Close]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 326.2 bits (835), Expect = 4.0e-88
Identity = 174/312 (55.77%), Postives = 213/312 (68.27%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK SRDKRDIYYR AKE  WRARSAFKL+QID+EF I +GV+R VDLCAAPGSWSQVLS
Sbjct: 1   MGKTSRDKRDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           ++LY         ++     IVAIDLQPMAPI GVIQ+QGDIT+  TA  VIKHF G K+
Sbjct: 61  KRLY---------EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKS 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           D+V+CDGAPDVTG+H +DEF+Q++LILA   I +HVL+EGG F+AKIFR +++SLLY Q+
Sbjct: 121 DIVICDGAPDVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQM 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           K +F  V  AKP+SSR SS EAF +C +YSPPEGF P              G   LD + 
Sbjct: 181 KKYFKKVYLAKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDATD 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPL------PRGAAG-----AYRSLDPVQPPIA 300
                P+ +   F+ CGDLSG+DS++SYPL      P+G         Y   D VQPP  
Sbjct: 241 ASAISPD-IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTD 290

Query: 301 PPYKRALEMKKA 302
           P YK AL+ KK+
Sbjct: 301 PAYKAALDKKKS 290

[Close]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.3e-83
Identity = 157/274 (57.30%), Postives = 202/274 (73.72%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK+S+DKRDIYYRKAKEEGWRARSAFKL+QIDEE+ IFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKSSKDKRDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           R++Y       D KD D+  IVA+DLQ MAP++GV+Q++GDIT   T++ +I HFDG  A
Sbjct: 61  RRIY------GDGKDPDVK-IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DL++ DGAPDVTGLHD+D + QSQLIL+ L I TH L+ GG F+AK+F+G D SL+Y Q+
Sbjct: 121 DLIISDGAPDVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQM 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLE---KVGSPSGGDDLD 240
           KLFF  V+F KP SSR SS+E F +C NY PP  +NPK +   LE   K+ + +  +  +
Sbjct: 181 KLFFEHVSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNN 240

Query: 241 CSSGWLEGPN--------KVYIPFLACGDLS-GY 263
            ++  ++  N         + +PF+ACGDL+ GY
Sbjct: 241 NNNCNIDNENDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.4e-79
Identity = 159/308 (51.62%), Postives = 212/308 (68.83%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFE--GVKRVVDLCAAPGSWSQV 60
           MGK+S+DKRD+YYRKAKE+G+RARSAFKLLQ++++F+  +   +KRVVDLCAAPGSWSQV
Sbjct: 1   MGKSSKDKRDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQV 60

Query: 61  LSRKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGC 120
           LSRKL+     SSD +D     IV++DLQPM+PI  V  +Q DIT+ +T   ++K F   
Sbjct: 61  LSRKLF-DESPSSDKEDRK---IVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNE 120

Query: 121 KADLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC 180
           KAD V  DGAPDVTGLHD+DE+VQ QLI++ L +   +L++GG F+AKIFRG+D  +LY 
Sbjct: 121 KADFVCSDGAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYS 180

Query: 181 QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK-DLHRLLEKVGSPSGGDDL- 240
           QL   F  +  AKP+SSR +S+EAF VC  Y+PP  + PK D++  +++       + L 
Sbjct: 181 QLGYLFDKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLC 240

Query: 241 --DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAAGAYRSLDPVQPPIAPPYK 300
             D  S W E    +   F+ACG L  +DSD +Y  LP   AG   SLDPVQ P  PPYK
Sbjct: 241 ISDKLSHWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYK 300

Query: 301 RALEMKKA 302
           +ALE+K++
Sbjct: 301 KALELKRS 303

[Close]

BLAST of Lag0027235 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.0e-176
Identity = 309/316 (97.78%), Postives = 311/316 (98.42%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS DSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Lag0027235 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.8e-175
Identity = 307/316 (97.15%), Postives = 310/316 (98.10%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS D KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Lag0027235 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-174
Identity = 307/316 (97.15%), Postives = 309/316 (97.78%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS DSKDGD PLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLS DS
Sbjct: 301 ASSQGIRELEKLSSDS 316

[Close]

BLAST of Lag0027235 vs. ExPASy TrEMBL
Match: A0A6J1CWI5 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111015042 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.9e-174
Identity = 308/316 (97.47%), Postives = 309/316 (97.78%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLSS SKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTA VVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSSVSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAGVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPS GDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSXGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA GAYRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGAYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQG R LEKLS DS
Sbjct: 301 ASSQGGRXLEKLSSDS 316

[Close]

BLAST of Lag0027235 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.4e-173
Identity = 305/316 (96.52%), Postives = 309/316 (97.78%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           RKLYLPAKLS D KDG+LPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY QL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSS
Sbjct: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAGAYRSLDPVQPPIAPPYKRALEMKK 300
           GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGA G YRSLDPVQPPIAPPYKRALEMKK
Sbjct: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 300

Query: 301 ASSQGIRELEKLSLDS 317
           ASSQGIRELEKLSLDS
Sbjct: 301 ASSQGIRELEKLSLDS 316

[Close]

BLAST of Lag0027235 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 5.2e-168
Identity = 286/305 (93.77%), Postives = 299/305 (98.03%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIKHFDGCKA 120
           R+LYLPAK S++SKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVI+HFDGCKA
Sbjct: 61  RQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180
           DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTH+L+EGGKFIAKIFRGKDTSLLYCQL
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSS 240
           KLFFP VTFAKPKSSRNSSIEAFAVCENYSPPEGFNP+DLHRLLEKVGSPSGG DLDCSS
Sbjct: 181 KLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSS 240

Query: 241 GWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAAG-AYRSLDPVQPPIAPPYKRALEMK 300
           GWLEGPNKVYIPFLACGDL+GYDSDRSYPLPR A G +Y+SLDP+QPPIAPPYKRALE+K
Sbjct: 241 GWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELK 300

Query: 301 KASSQ 305
           KAS+Q
Sbjct: 301 KASAQ 305

[Close]

BLAST of Lag0027235 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.0e-30
Identity = 81/240 (33.75%), Postives = 132/240 (55.00%), Query Frame = 0


Query: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60
           MGK   + + D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W QV 
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  SRKLYLPAKLSSDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDIT----NARTAEVVIKHF 120
             K+ + +            L++ IDL P+ P+ G + +  DIT     ++  +V+ +H 
Sbjct: 61  VEKVPVGS------------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH- 120

Query: 121 DGCKA-DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKD-T 180
            G  A +LV+ DG+P+V G    +   Q+ L++  + + T  L   G  + K+FR +D  
Sbjct: 121 -GVSAFNLVLHDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYN 180

Query: 181 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK--DLHRLLEKVGSPS 232
           S+LYC  +LF  V  F KP +SR++S E + V   Y  P   +P+  D   L ++   P+
Sbjct: 181 SVLYCLGRLFEKVEVF-KPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

[Close]

BLAST of Lag0027235 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-27
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = 0


Query: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 58
          MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of Lag0027235 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 72.4 bits (176), Expect = 7.3e-13
Identity = 55/225 (24.44%), Postives = 98/225 (43.56%), Query Frame = 0


Query: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69
           D +YR+A+  G+ ARSAFKLLQI +++ + +    V+DL  APG+W QV  + L     L
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSL---GPL 67

Query: 70  SSDSKDGDLPLIVAIDLQ----PMAPIEGVIQVQGDITNARTAEVVIKHFDGCKADLVVC 129
            S        ++V +D++    P      V  +  D+ N    ++           +++ 
Sbjct: 68  RSGG------IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILS 127

Query: 130 DGAPDVTGLHDMDEFVQSQLILAGLTIVT---------------------HVLREGGKFI 189
           D    V+G+   D  + ++L +  L +                        VLR GG  +
Sbjct: 128 DMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGHLV 187

Query: 190 AKIFRGKDTSLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 210
            K+   +D        K  F   ++ +PK++R SS E + +C+ +
Sbjct: 188 IKLLESEDAQDFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of Lag0027235 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903231.1	4.9e-176	97.78	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]
KAG6580486.1	8.3e-176	97.78	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	8.3e-176	97.78	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]
XP_008442455.1	1.2e-174	97.15	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]
XP_022983316.1	5.9e-174	97.15	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita max...	[more]

BLAST of Lag0027235 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	5.5e-98	58.67	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
O36015	6.1e-89	56.15	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q22031	4.0e-88	55.77	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
Q54VA8	2.3e-83	57.30	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	4.4e-79	51.62	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of Lag0027235 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1P7	4.0e-176	97.78	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A1S3B6C8	5.8e-175	97.15	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A6J1IYY9	2.9e-174	97.15	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A6J1CWI5	4.9e-174	97.47	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Momordica c...	[more]
A0A0A0LCV6	1.4e-173	96.52	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]

BLAST of Lag0027235 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	5.2e-168	93.77	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	1.0e-30	33.75	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	3.1e-27	100.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	7.3e-13	24.44	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 21..209 e-value: 1.8E-64 score: 217.2
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 20..215 e-value: 1.6E-73 score: 248.6
None	No IPR available	PIRSR	PIRSR005461-1	PIRSR005461-1	coord: 2..211 e-value: 6.6E-67 score: 223.0
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 1..308
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 1..308
IPR015507	Ribosomal RNA large subunit methyltransferase E	HAMAP	MF_01547	RNA_methyltr_E	coord: 5..210 score: 32.271809
IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7	HAMAP	MF_03162	RNA_methyltr_E_TRM7	coord: 1..268 score: 33.979515
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 20..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027235.1	Lag0027235.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0002128	tRNA nucleoside ribose methylation
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008175	tRNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity