Lag0027227 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0027227 vs. NCBI nr
Match: XP_038903136.1 (protein OCTOPUS-like [Benincasa hispida])

HSP 1 Score: 984.9 bits (2545), Expect = 3.2e-283
Identity = 542/664 (81.63%), Postives = 575/664 (86.60%), Query Frame = 0

Query: 1   MNPITDAQPA--------PPLPHR---PCPRHPQEHFTGFCPLCLCERLSLLDSSSSRKP 60
           MNP TD QPA        PPLPHR   PCPRHPQEHFT FCPLCLCERLS+LDSS+S   
Sbjct: 1   MNPSTDEQPAPPPLPPPLPPLPHRPSAPCPRHPQEHFTAFCPLCLCERLSVLDSSASAAS 60

Query: 61  PSSS----SSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCD 120
            SSS    S+++SAL++IFRP   +RPSSFFPELRR KSFSASKNE FS VFEPQRKSCD
Sbjct: 61  SSSSRKPHSTAASALRAIFRPAPPNRPSSFFPELRRAKSFSASKNEAFSAVFEPQRKSCD 120

Query: 121 VRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESN 180
           VR+RNTL SLFSQD S SS+++      G EI  ES+NLE+  SSCL        E + +
Sbjct: 121 VRLRNTLCSLFSQDASASSRLL---AAPGPEIILESKNLEDPISSCL--------EPQPD 180

Query: 181 TNGEIRVSEEANVVDVVIENGVQEIV--EEEEIEAELGPESVQLQEEFRTMKDHIDLDSH 240
            +GEIRVS + NVVDVVIENGVQEIV  EEEEI+ ELG ESVQLQEEF+TMKDHIDLDSH
Sbjct: 181 GDGEIRVSGQPNVVDVVIENGVQEIVEEEEEEIQVELGSESVQLQEEFKTMKDHIDLDSH 240

Query: 241 TKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRE 300
           TKKP+GR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFRE
Sbjct: 241 TKKPAGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRE 300

Query: 301 TQSEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTML 360
           TQSEIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTML
Sbjct: 301 TQSEIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTML 360

Query: 361 SVVEDAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSI 420
           SVVEDAPI+VFRSDTQIPVEDSINSTNE+ENIPGGSSQTRDYYSDSSSRRRKSLDRSNSI
Sbjct: 361 SVVEDAPIHVFRSDTQIPVEDSINSTNEDENIPGGSSQTRDYYSDSSSRRRKSLDRSNSI 420

Query: 421 RKTAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF 480
           RKTAAAVVAEID+MK SVSNAKVSPATTD+CHGPKL IPDRDSNSNS+RDDCSGSFD   
Sbjct: 421 RKTAAAVVAEIDDMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSNSMRDDCSGSFD--- 480

Query: 481 DAASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESW 540
           DAASV+GTGNRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESW
Sbjct: 481 DAASVVGTGNRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESW 540

Query: 541 PELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIG-GTFSSSRKSNADSNGNGNGNGNGN 600
           PELRG+RN DVKGGFNPKMFRSNSSVSWRS+SM+G G FSSSRKSNA+S        NGN
Sbjct: 541 PELRGDRNGDVKGGFNPKMFRSNSSVSWRSSSMMGSGYFSSSRKSNAES--------NGN 600

Query: 601 GKKKKDE--PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSV 643
           GKKKK+E  PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSV
Sbjct: 601 GKKKKEEPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSV 636

BLAST of Lag0027227 vs. NCBI nr
Match: XP_022934120.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 974.5 bits (2518), Expect = 4.3e-280
Identity = 530/656 (80.79%), Postives = 566/656 (86.28%), Query Frame = 0

Query: 1   MNPITDAQPA-----PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSS 60
           MNP T  QPA     PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+    S+
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  ---SSSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDVRVR 120
               S+++SALK+IFRP   +RPSSFFPELRRTKSFSASKN+ FS VFEPQRKSCDVRVR
Sbjct: 61  RKPHSTAASALKAIFRPAPPNRPSSFFPELRRTKSFSASKNDAFSAVFEPQRKSCDVRVR 120

Query: 121 NTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNTNGE 180
           N   SLFS D S SS +V       AEI  E++ LE++SSSCL        E     +GE
Sbjct: 121 NAHCSLFSHDASASSNLV----APPAEITPETKILEDSSSSCL--------ESHPVGDGE 180

Query: 181 IRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPSG 240
           I+VS+  NVVDVVIENGVQEIVEEEEI  E+GPE VQLQEEF+TMKDHIDLDSHTKKPSG
Sbjct: 181 IKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTKKPSG 240

Query: 241 RDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEIA 300
           R      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFR+TQSEIA
Sbjct: 241 R------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQSEIA 300

Query: 301 DYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA 360
           DYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA
Sbjct: 301 DYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA 360

Query: 361 PINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA 420
           PINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA
Sbjct: 361 PINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA 420

Query: 421 VVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-DAASV 480
           VVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL++DCS SFDS F DAASV
Sbjct: 421 VVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFNDAASV 480

Query: 481 IGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPELRG 540
           +GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWPELRG
Sbjct: 481 VGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWPELRG 540

Query: 541 ERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKKKKD 600
           ERNSDVKGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSNA++        NGNG+KK +
Sbjct: 541 ERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET--------NGNGRKKNE 600

Query: 601 EPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLERNRSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of Lag0027227 vs. NCBI nr
Match: KAG6580491.1 (Protein OCTOPUS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 964.9 bits (2493), Expect = 3.4e-277
Identity = 528/660 (80.00%), Postives = 564/660 (85.45%), Query Frame = 0

Query: 1   MNPITDAQPA---------PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRK 60
           MNP T  QPA         PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+  
Sbjct: 1   MNPSTHEQPAPPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTS 60

Query: 61  PPSS---SSSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCD 120
             S+    S+++SALK+IFRP   +RPSSFFPELRRTKSFSASKN+ FS VFEPQRKSCD
Sbjct: 61  SSSTRKPHSTAASALKAIFRPAPPNRPSSFFPELRRTKSFSASKNDAFSAVFEPQRKSCD 120

Query: 121 VRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESN 180
           VRVRN   SLFS D S SS +V       AEI  E++ LE++SSSCL        E    
Sbjct: 121 VRVRNAHCSLFSHDASASSNLV----APPAEITPETKILEDSSSSCL--------EPYPV 180

Query: 181 TNGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTK 240
            +GEIRVS++ NVVDVVIENGVQEIVEEEEI+ E+GPE VQLQEEF+TMKDHIDLDSHTK
Sbjct: 181 GDGEIRVSKQPNVVDVVIENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTK 240

Query: 241 KPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQ 300
           KPSGR      GSFWSAASVFSKKLQKWRDKQKEKK RNG GSTTLPVEKPIGRHFR+TQ
Sbjct: 241 KPSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQ 300

Query: 301 SEIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSV 360
           SEIADYG+GRRSCD DPRFSLDAGRMS DDPRYSFDEPRASWDGYLISRT PRMPTMLSV
Sbjct: 301 SEIADYGYGRRSCDIDPRFSLDAGRMSIDDPRYSFDEPRASWDGYLISRTLPRMPTMLSV 360

Query: 361 VEDAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420
           VEDAPINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK
Sbjct: 361 VEDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRK 420

Query: 421 TAAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-D 480
           TAAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL++DCS S DS F D
Sbjct: 421 TAAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFND 480

Query: 481 AASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWP 540
           AASV+GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWP
Sbjct: 481 AASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWP 540

Query: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGK 600
           ELRGERNSDVKGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSNA++        NGNGK
Sbjct: 541 ELRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET--------NGNGK 600

Query: 601 KKKDEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           KK +EPVLERNRSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 KKNEEPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 634

BLAST of Lag0027227 vs. NCBI nr
Match: XP_022983305.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 963.8 bits (2490), Expect = 7.6e-277
Identity = 524/657 (79.76%), Postives = 564/657 (85.84%), Query Frame = 0

Query: 1   MNPITDAQPA-----PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSS 60
           MNP T  QPA     PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+    SS
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSSS 60

Query: 61  S----SSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDVRV 120
           +    S+++SALK+IFRP   +RPSSFFPELRRTKSFSASKN+ FS VFEPQRKSCDVRV
Sbjct: 61  TRKPHSTAASALKAIFRPAPPNRPSSFFPELRRTKSFSASKNDAFSAVFEPQRKSCDVRV 120

Query: 121 RNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNTNG 180
           RN   SLFS D S SS +V       AEI  E++ LE++SSSCL        E +   + 
Sbjct: 121 RNAHCSLFSHDASASSNLV----APSAEITPETKFLEDSSSSCL--------EHQPLGHR 180

Query: 181 EIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPS 240
           EIRVSE+ NVVDVVIENG QEIVEE+EI+ E+GPE+VQLQEEF+TMKDHIDLDSHTKKPS
Sbjct: 181 EIRVSEQPNVVDVVIENGAQEIVEEKEIQVEVGPETVQLQEEFKTMKDHIDLDSHTKKPS 240

Query: 241 GRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEI 300
           GR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGST LPVEKPIGRHFR+TQSEI
Sbjct: 241 GR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTALPVEKPIGRHFRDTQSEI 300

Query: 301 ADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED 360
           ADYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED
Sbjct: 301 ADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED 360

Query: 361 APINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA 420
           APINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA
Sbjct: 361 APINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA 420

Query: 421 AVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-DAAS 480
           AVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL++DCS S DS F D AS
Sbjct: 421 AVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFNDVAS 480

Query: 481 VIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPELR 540
           V+GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERS S SWPELR
Sbjct: 481 VVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSNSGSWPELR 540

Query: 541 GERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKKKK 600
           GERNSD+KGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSN ++        NGNGKKK 
Sbjct: 541 GERNSDIKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNTET--------NGNGKKKN 600

Query: 601 DEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           +EPVLER+RSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EEPVLERSRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 631

BLAST of Lag0027227 vs. NCBI nr
Match: XP_023526761.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.4 bits (2489), Expect = 9.9e-277
Identity = 526/659 (79.82%), Postives = 564/659 (85.58%), Query Frame = 0

Query: 1   MNPITDAQPA--------PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKP 60
           MNP T  +PA        PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+   
Sbjct: 1   MNPSTHEKPAPPPPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSS 60

Query: 61  PSS---SSSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDV 120
            S+    S+++SALK+IFRP   +RPS F PELRRTKSFSASKN+ FS VFEPQRKSCDV
Sbjct: 61  SSTRKPHSTAASALKAIFRPAPPNRPSFFLPELRRTKSFSASKNDAFSAVFEPQRKSCDV 120

Query: 121 RVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNT 180
           RVRN   SLFS D S SS +V       AEI +E++ LE++SSSCL        E     
Sbjct: 121 RVRNAHCSLFSHDASASSNIV----APPAEITSETKILEDSSSSCL--------EPHPVG 180

Query: 181 NGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKK 240
           +GEIRVS++ NVVDVV ENGVQEIVEEEEI+ E+GPE VQLQEEF+TMKDHIDLDSHTKK
Sbjct: 181 DGEIRVSKQPNVVDVVNENGVQEIVEEEEIQVEVGPEPVQLQEEFKTMKDHIDLDSHTKK 240

Query: 241 PSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQS 300
           PSGR      GSFWSAASVFSKKLQKWRDKQKEKK RNG GSTTLPVEKPIGRHFR+TQS
Sbjct: 241 PSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGVGSTTLPVEKPIGRHFRDTQS 300

Query: 301 EIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360
           EIADYG+GRRSCD DPRFSLDAGR+SFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV
Sbjct: 301 EIADYGYGRRSCDIDPRFSLDAGRISFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360

Query: 361 EDAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420
           EDAPINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT
Sbjct: 361 EDAPINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420

Query: 421 AAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-DA 480
           AAAVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL+DDCS S DS F DA
Sbjct: 421 AAAVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQDDCSKSLDSAFNDA 480

Query: 481 ASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPE 540
           ASV+GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWPE
Sbjct: 481 ASVVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWPE 540

Query: 541 LRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKK 600
           LRGERNSDVKGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSNA++        NGNGKK
Sbjct: 541 LRGERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET--------NGNGKK 600

Query: 601 KKDEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           K +EPVLERNRSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 KNEEPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 633

BLAST of Lag0027227 vs. ExPASy Swiss-Prot
Match: Q9SS80 (Protein OCTOPUS OS=Arabidopsis thaliana OX=3702 GN=OPS PE=1 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 7.7e-139
Identity = 363/746 (48.66%), Postives = 437/746 (58.58%), Query Frame = 0

Query: 1   MNPITD----------AQPAPPLPHR---PCPRHPQEHFTGFCPLCLCERLSLLD----- 60
           MNP TD            P PP PHR    C RHP+E FTGFCP CLCERLS+LD     
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  --SSSSRKPPSSSSSSSSALKSIFRP------------GAASRPSSFFPELRRTKSFSAS 120
             SSSS+KPP   + S++ALK++F+P            G       FFPELRRTKSFSAS
Sbjct: 61  GSSSSSKKPP---TISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFSAS 120

Query: 121 K-NEGFSGVFEPQRKSCDVRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEET 180
           K NEGFSGVFEPQR+SCDVR+R++LW+LFSQD   +    V GG    EI  E R     
Sbjct: 121 KNNEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGG----EIDVEPR----- 180

Query: 181 SSSCLGGPVLEA-EEGESNTNG-EIRVSEEANVVDV-----------VIENGVQEIVE-E 240
             S +  PVLE  +EGE+ ++  E+   EE + V+            ++     EIVE  
Sbjct: 181 -KSSVAEPVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVR 240

Query: 241 EEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPSGRDLKEIAGSFWSAASVFSKKLQKW 300
           EEIE  + P     +EE + +KD+IDLDS TKKPS      +  SFWSAASVFSKKLQKW
Sbjct: 241 EEIEEAVKPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKW 300

Query: 301 RDKQKEKKHRNGG----GSTTLPVEKPIGRHFRETQSEIADYGFGRRSCDTDPRFSLDA- 360
           R  QK KK RNGG    GS  LPVEKPIGR  R+TQSEIADYG+GRRSCDTDPRFSLDA 
Sbjct: 301 RQNQKMKKRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAG 360

Query: 361 -------------GRMSFDDPRYSFDEPRASWDGYLISRTF-------PRMPTMLSVVED 420
                        GR+S DDPRYSFDEPRASWDG LI RT        P  P+MLSVVED
Sbjct: 361 RFSLDAGRFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVED 420

Query: 421 AP----INVFRSDTQIPVEDS------INSTNEEEN---IPGGSSQTRDYYSDSSSRRRK 480
           AP     +V R+D Q PVE+       +N TN   +   IPGGS QTRDYY+DSSSRRRK
Sbjct: 421 APPPVHRHVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRK 480

Query: 481 SLDR-SNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDC 540
           SLDR S+S+RKTAAAVVA++DE K      VS A +   +   L    RD+N+ ++    
Sbjct: 481 SLDRSSSSMRKTAAAVVADMDEPK----LSVSSAISIDAYSGSL----RDNNNYAVETAD 540

Query: 541 SGSFDSGFDAASVIGTG--NRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRE 600
           +GSF    + A +IG    N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR 
Sbjct: 541 NGSFR---EPAMMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRY 600

Query: 601 SRPNG--VERSYSESWPELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKS 643
            R NG  VERS SESWPELR        GG  P+M RSNS+VSWRS              
Sbjct: 601 RRLNGGMVERSLSESWPELRNGGG----GGGGPRMVRSNSNVSWRS-------------- 660

BLAST of Lag0027227 vs. ExPASy Swiss-Prot
Match: Q9LFB9 (Protein OCTOPUS-like OS=Arabidopsis thaliana OX=3702 GN=OPSL1 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 8.0e-104
Identity = 315/697 (45.19%), Postives = 380/697 (54.52%), Query Frame = 0

Query: 3   PIT--DAQPAPPLPHR---PCPRHPQEHFTGFCPLCLCERLSLLD-------SSSSRKPP 62
           P+T  D    P  PHR    C  HP+E F+GFCP CLC+RLS+LD       SSSSRKPP
Sbjct: 9   PVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPPSSSSRKPP 68

Query: 63  SSSSSSSSALKSIFRPGAASRPSS---------FFPELRRTKSFSASKNEGFSGVFEPQR 122
           S S+ S   LK++F+P ++   +S         FFPELRRTKSFSA  NEGFSG FEPQR
Sbjct: 69  SISAVS---LKALFKPSSSGTNNSNGNGRVRPGFFPELRRTKSFSAKNNEGFSGGFEPQR 128

Query: 123 KSCDVRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEE 182
           +SCDVR+R+   +L   + +   K+                  EE   S +   VLE  E
Sbjct: 129 RSCDVRLRDDERNLPINEAASVDKIE-----------------EEARESSVSEIVLEVTE 188

Query: 183 GESNTNGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLD 242
                  EI   EE        E    EIVEE+   +E+G E    +EE + MKD++DL 
Sbjct: 189 -----EAEIEEDEENG------EKDPGEIVEEK--SSEIGEE----EEELKPMKDYMDLY 248

Query: 243 SHTKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHF 302
           S TKKPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR  
Sbjct: 249 SQTKKPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR-- 308

Query: 303 RETQSEIADYGFGRRSCDTDPRFSLDA-------GRMSFDDPRYSFDEPRASWDGYLISR 362
              QSEI   G GRRS DTDPRFSLDA       GR+S DD RYS DEPRASWDG+LI R
Sbjct: 309 --PQSEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGR 368

Query: 363 T----FPRMPTMLSVVEDAPINVFRSDTQIPVEDSINSTNEEEN----IPGGSSQTRDYY 422
           T     P  P+MLSVVE+AP+N  RSD QIP   SI   + + +    IPGGS+QTRDYY
Sbjct: 369 TTAARVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYY 428

Query: 423 S-DSSSRRRKSLDRSNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDS 482
           +   SSRRRKSLDRSNSIRK    +V E++++KSVSN     +TT I           DS
Sbjct: 429 TGPPSSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DS 488

Query: 483 NSNSLRDDCSGSFDSGFDAASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEED 542
           NS    ++                 GN+  + KKSRRWGK W+I G I R+ G  DEEED
Sbjct: 489 NSMETAEN----------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEED 548

Query: 543 RESRPNG---VERSYSESWPELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSS 602
           R SR N    VERS SESWPE+R         G  PKM RSNS+VSWRS           
Sbjct: 549 RYSRSNSAGMVERSLSESWPEMRNGE------GGGPKMRRSNSNVSWRS----------- 568

Query: 603 RKSNADSNGNGNGNGNGNGKKKKDEPVLERNRSARHSPTNVDNGLLRFYLTPLR------ 643
                        +G G+           RN+S+R+S  + +NG+LRFYLTP+R      
Sbjct: 609 -------------SGGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTS 568

BLAST of Lag0027227 vs. ExPASy TrEMBL
Match: A0A6J1F6S2 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441390 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.1e-280
Identity = 530/656 (80.79%), Postives = 566/656 (86.28%), Query Frame = 0

Query: 1   MNPITDAQPA-----PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSS 60
           MNP T  QPA     PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+    S+
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSST 60

Query: 61  ---SSSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDVRVR 120
               S+++SALK+IFRP   +RPSSFFPELRRTKSFSASKN+ FS VFEPQRKSCDVRVR
Sbjct: 61  RKPHSTAASALKAIFRPAPPNRPSSFFPELRRTKSFSASKNDAFSAVFEPQRKSCDVRVR 120

Query: 121 NTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNTNGE 180
           N   SLFS D S SS +V       AEI  E++ LE++SSSCL        E     +GE
Sbjct: 121 NAHCSLFSHDASASSNLV----APPAEITPETKILEDSSSSCL--------ESHPVGDGE 180

Query: 181 IRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPSG 240
           I+VS+  NVVDVVIENGVQEIVEEEEI  E+GPE VQLQEEF+TMKDHIDLDSHTKKPSG
Sbjct: 181 IKVSKRPNVVDVVIENGVQEIVEEEEIRVEVGPEPVQLQEEFKTMKDHIDLDSHTKKPSG 240

Query: 241 RDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEIA 300
           R      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFR+TQSEIA
Sbjct: 241 R------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRDTQSEIA 300

Query: 301 DYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA 360
           DYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA
Sbjct: 301 DYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDA 360

Query: 361 PINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA 420
           PINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA
Sbjct: 361 PINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAA 420

Query: 421 VVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-DAASV 480
           VVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL++DCS SFDS F DAASV
Sbjct: 421 VVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSFDSAFNDAASV 480

Query: 481 IGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPELRG 540
           +GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERSYS SWPELRG
Sbjct: 481 VGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSYSGSWPELRG 540

Query: 541 ERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKKKKD 600
           ERNSDVKGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSNA++        NGNG+KK +
Sbjct: 541 ERNSDVKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNAET--------NGNGRKKNE 600

Query: 601 EPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           EPVLERNRSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EPVLERNRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 630

BLAST of Lag0027227 vs. ExPASy TrEMBL
Match: A0A6J1J5H7 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481925 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.7e-277
Identity = 524/657 (79.76%), Postives = 564/657 (85.84%), Query Frame = 0

Query: 1   MNPITDAQPA-----PPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSS 60
           MNP T  QPA     PPLPHRP   CPRHPQE FT FCPLCLCERLSLLDSSS+    SS
Sbjct: 1   MNPSTHEQPAPPPPLPPLPHRPSAACPRHPQEQFTAFCPLCLCERLSLLDSSSTTSSSSS 60

Query: 61  S----SSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDVRV 120
           +    S+++SALK+IFRP   +RPSSFFPELRRTKSFSASKN+ FS VFEPQRKSCDVRV
Sbjct: 61  TRKPHSTAASALKAIFRPAPPNRPSSFFPELRRTKSFSASKNDAFSAVFEPQRKSCDVRV 120

Query: 121 RNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNTNG 180
           RN   SLFS D S SS +V       AEI  E++ LE++SSSCL        E +   + 
Sbjct: 121 RNAHCSLFSHDASASSNLV----APSAEITPETKFLEDSSSSCL--------EHQPLGHR 180

Query: 181 EIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPS 240
           EIRVSE+ NVVDVVIENG QEIVEE+EI+ E+GPE+VQLQEEF+TMKDHIDLDSHTKKPS
Sbjct: 181 EIRVSEQPNVVDVVIENGAQEIVEEKEIQVEVGPETVQLQEEFKTMKDHIDLDSHTKKPS 240

Query: 241 GRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEI 300
           GR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGST LPVEKPIGRHFR+TQSEI
Sbjct: 241 GR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTALPVEKPIGRHFRDTQSEI 300

Query: 301 ADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED 360
           ADYG+GRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED
Sbjct: 301 ADYGYGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVED 360

Query: 361 APINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA 420
           APINVFR+D QIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA
Sbjct: 361 APINVFRTDAQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAA 420

Query: 421 AVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGF-DAAS 480
           AVVAEIDEMK SVSNAKVSPATTD+CHGPKL IPDRDSNS+SL++DCS S DS F D AS
Sbjct: 421 AVVAEIDEMKSSVSNAKVSPATTDVCHGPKLAIPDRDSNSSSLQEDCSKSLDSAFNDVAS 480

Query: 481 VIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPELR 540
           V+GTGNRKEESKKSR WGK W IWGLINRRGGNKDEEED+E SRPNG+ERS S SWPELR
Sbjct: 481 VVGTGNRKEESKKSRGWGKGWRIWGLINRRGGNKDEEEDKESSRPNGMERSNSGSWPELR 540

Query: 541 GERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKKKK 600
           GERNSD+KGGFNPKMFRSNSSVSWRS+SM+GG+FSSSRKSN ++        NGNGKKK 
Sbjct: 541 GERNSDIKGGFNPKMFRSNSSVSWRSSSMMGGSFSSSRKSNTET--------NGNGKKKN 600

Query: 601 DEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           +EPVLER+RSARHSPTN+DNGLLRFYLT LRGSRRGGSGKVKPNQAQSIARSVLRLY
Sbjct: 601 EEPVLERSRSARHSPTNIDNGLLRFYLTSLRGSRRGGSGKVKPNQAQSIARSVLRLY 631

BLAST of Lag0027227 vs. ExPASy TrEMBL
Match: A0A5A7TLQ7 (UPF0503 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001040 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.5e-275
Identity = 534/661 (80.79%), Postives = 564/661 (85.33%), Query Frame = 0

Query: 1   MNPITDAQ----PAPPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSSS 60
           MNP TD Q    P PPLPHRP   CPRHPQEHFT FCPLCLCERLSLLDSSS+    SSS
Sbjct: 1   MNPSTDGQPLPPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSSS 60

Query: 61  SSS-------SSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDV 120
           SSS       +SALK+IFRP   +RPSSFFPELRRTKSFSASKNE FS VFEPQRKSCDV
Sbjct: 61  SSSRKPHSTAASALKAIFRPPPPNRPSSFFPELRRTKSFSASKNEAFSTVFEPQRKSCDV 120

Query: 121 RVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNT 180
           R+RNTL SL SQD S SSKV+        EI  E++NLE+  SS +        E   + 
Sbjct: 121 RLRNTLCSLISQDASSSSKVL---APAAPEIVVETKNLEDPCSSWV--------EPIPDG 180

Query: 181 NGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKK 240
           +G+IRVS + NV D VIEN VQEIV EEEI+ EL  ESV LQEEF+TMKDHIDLDSHTKK
Sbjct: 181 DGDIRVSGQPNVGDFVIENSVQEIV-EEEIQVELESESVHLQEEFKTMKDHIDLDSHTKK 240

Query: 241 PSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQS 300
           PSGR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFRETQS
Sbjct: 241 PSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRETQS 300

Query: 301 EIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360
           EIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV
Sbjct: 301 EIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360

Query: 361 EDAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420
           EDAPI+VFRSDTQIPVEDSINSTNEEENIPGGSSQTR+YYSDSSSRRRKSLDRSNSIRKT
Sbjct: 361 EDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNSIRKT 420

Query: 421 AAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGFDAA 480
           AAAVVAEID+MK SVSNAKVSPATTD+CH PKLG+PDRDSNSNSLRDD SGSF+   DAA
Sbjct: 421 AAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFE---DAA 480

Query: 481 SVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPEL 540
           SV+GT NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPEL
Sbjct: 481 SVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPEL 540

Query: 541 RGERNSDVK-GGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKK 600
           RG+RN DVK GGFNPKMFRSNSSVSWRSASM+GG+FSSSRKSNA+S        NGNGKK
Sbjct: 541 RGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAES--------NGNGKK 600

Query: 601 KKDE--PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRL 643
           KK+E  PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKP+QAQSIARSVLRL
Sbjct: 601 KKEEPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRL 632

BLAST of Lag0027227 vs. ExPASy TrEMBL
Match: A0A1S3B6I0 (UPF0503 protein At3g09070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486324 PE=4 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.5e-275
Identity = 534/661 (80.79%), Postives = 564/661 (85.33%), Query Frame = 0

Query: 1   MNPITDAQ----PAPPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSSS 60
           MNP TD Q    P PPLPHRP   CPRHPQEHFT FCPLCLCERLSLLDSSS+    SSS
Sbjct: 1   MNPSTDGQPLPPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAAAASSSS 60

Query: 61  SSS-------SSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFSGVFEPQRKSCDV 120
           SSS       +SALK+IFRP   +RPSSFFPELRRTKSFSASKNE FS VFEPQRKSCDV
Sbjct: 61  SSSRKPHSTAASALKAIFRPPPPNRPSSFFPELRRTKSFSASKNEAFSTVFEPQRKSCDV 120

Query: 121 RVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNT 180
           R+RNTL SL SQD S SSKV+        EI  E++NLE+  SS +        E   + 
Sbjct: 121 RLRNTLCSLISQDASSSSKVL---APAAPEIVVETKNLEDPCSSWV--------EPIPDG 180

Query: 181 NGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKK 240
           +G+IRVS + NV D VIEN VQEIV EEEI+ EL  ESV LQEEF+TMKDHIDLDSHTKK
Sbjct: 181 DGDIRVSGQPNVGDFVIENSVQEIV-EEEIQVELESESVHLQEEFKTMKDHIDLDSHTKK 240

Query: 241 PSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQS 300
           PSGR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFRETQS
Sbjct: 241 PSGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRETQS 300

Query: 301 EIADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360
           EIADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV
Sbjct: 301 EIADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVV 360

Query: 361 EDAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKT 420
           EDAPI+VFRSDTQIPVEDSINSTNEEENIPGGSSQTR+YYSDSSSRRRKSLDRSNSIRKT
Sbjct: 361 EDAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNSIRKT 420

Query: 421 AAAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGFDAA 480
           AAAVVAEID+MK SVSNAKVSPATTD+CH PKLG+PDRDSNSNSLRDD SGSF+   DAA
Sbjct: 421 AAAVVAEIDDMKSSVSNAKVSPATTDVCHVPKLGLPDRDSNSNSLRDDYSGSFE---DAA 480

Query: 481 SVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPEL 540
           SV+GT NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPEL
Sbjct: 481 SVVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPEL 540

Query: 541 RGERNSDVK-GGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKK 600
           RG+RN DVK GGFNPKMFRSNSSVSWRSASM+GG+FSSSRKSNA+S        NGNGKK
Sbjct: 541 RGDRNGDVKAGGFNPKMFRSNSSVSWRSASMMGGSFSSSRKSNAES--------NGNGKK 600

Query: 601 KKDE--PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRL 643
           KK+E  PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKP+QAQSIARSVLRL
Sbjct: 601 KKEEPQPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPSQAQSIARSVLRL 632

BLAST of Lag0027227 vs. ExPASy TrEMBL
Match: A0A0A0LAA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G732510 PE=4 SV=1)

HSP 1 Score: 953.4 bits (2463), Expect = 5.0e-274
Identity = 532/660 (80.61%), Postives = 567/660 (85.91%), Query Frame = 0

Query: 1   MNPITDAQ----PAPPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLDSSSSRKPPSSS 60
           MNP TD Q    P PPLPHRP   CPRHPQEHFT FCPLCLCERLSLLDSSS+    SSS
Sbjct: 1   MNPSTDGQPLPPPLPPLPHRPSATCPRHPQEHFTAFCPLCLCERLSLLDSSSAASSSSSS 60

Query: 61  -----SSSSSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFS-GVFEPQRKSCDVR 120
                S++SSAL+++FRP   +RPSSFFPELRRTKSFSASKNE FS  +FEPQRKSCDVR
Sbjct: 61  SRKPHSTASSALRALFRPPPPNRPSSFFPELRRTKSFSASKNEAFSTAIFEPQRKSCDVR 120

Query: 121 VRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEEGESNTN 180
           +RNTL SL SQD S SSK++       +EIA E++NLE+ SSS +        E   + +
Sbjct: 121 LRNTLCSLISQDASSSSKLL---APAASEIAVETKNLEDPSSSWV--------EPIPDVD 180

Query: 181 GEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKP 240
           G+IRVS + NV D VIEN V+EIV EEEI+ ELG ESVQLQEEF+TMKDHIDLDSHTKKP
Sbjct: 181 GDIRVSGQPNVGDFVIENSVKEIV-EEEIQVELGSESVQLQEEFKTMKDHIDLDSHTKKP 240

Query: 241 SGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSE 300
           SGR      GSFWSAASVFSKKLQKWRDKQKEKK RNGGGSTTLPVEKPIGRHFRETQSE
Sbjct: 241 SGR------GSFWSAASVFSKKLQKWRDKQKEKKQRNGGGSTTLPVEKPIGRHFRETQSE 300

Query: 301 IADYGFGRRSCDTDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVE 360
           IADYGFGRRSCD DPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVE
Sbjct: 301 IADYGFGRRSCDIDPRFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVE 360

Query: 361 DAPINVFRSDTQIPVEDSINSTNEEENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTA 420
           DAPI+VFRSDTQIPVEDSINSTNEEENIPGGSSQTR+YYSDSSSRRRKSLDRSNSIRKTA
Sbjct: 361 DAPIHVFRSDTQIPVEDSINSTNEEENIPGGSSQTREYYSDSSSRRRKSLDRSNSIRKTA 420

Query: 421 AAVVAEIDEMK-SVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDCSGSFDSGFDAAS 480
           AAVVAEID+MK SVSNAKVSPATTD+ HGPKLGIPDRDSNSNSLRDD SGSF+   DAAS
Sbjct: 421 AAVVAEIDDMKSSVSNAKVSPATTDVSHGPKLGIPDRDSNSNSLRDDYSGSFE---DAAS 480

Query: 481 VIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRE-SRPNGVERSYSESWPELR 540
           V+GT NRKEESKKS+ WGK W IWGLINRRGGNKDEEEDRE SRPNGVERSYSESWPELR
Sbjct: 481 VVGTANRKEESKKSKGWGKGWKIWGLINRRGGNKDEEEDRESSRPNGVERSYSESWPELR 540

Query: 541 GERNSDVK-GGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKKK 600
           G+RN DVK GGFNPKMFRSNSSVSWRSASMIGG+FSSSRKSNA+S        NGNGKKK
Sbjct: 541 GDRNGDVKAGGFNPKMFRSNSSVSWRSASMIGGSFSSSRKSNAES--------NGNGKKK 600

Query: 601 KDE--PVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKPNQAQSIARSVLRLY 643
           K+E  PVLERNRSAR SPTNVDNGLLRFYLTPLRGSRRG SGKVKP+QAQSIARSVLRLY
Sbjct: 601 KEEAQPVLERNRSARQSPTNVDNGLLRFYLTPLRGSRRGESGKVKPSQAQSIARSVLRLY 631

BLAST of Lag0027227 vs. TAIR 10
Match: AT3G09070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 495.7 bits (1275), Expect = 5.5e-140
Identity = 363/746 (48.66%), Postives = 437/746 (58.58%), Query Frame = 0

Query: 1   MNPITD----------AQPAPPLPHR---PCPRHPQEHFTGFCPLCLCERLSLLD----- 60
           MNP TD            P PP PHR    C RHP+E FTGFCP CLCERLS+LD     
Sbjct: 1   MNPATDPVSAAAAALAPPPQPPQPHRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNG 60

Query: 61  --SSSSRKPPSSSSSSSSALKSIFRP------------GAASRPSSFFPELRRTKSFSAS 120
             SSSS+KPP   + S++ALK++F+P            G       FFPELRRTKSFSAS
Sbjct: 61  GSSSSSKKPP---TISAAALKALFKPSGNNGVGGVNTNGNGRVKPGFFPELRRTKSFSAS 120

Query: 121 K-NEGFSGVFEPQRKSCDVRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEET 180
           K NEGFSGVFEPQR+SCDVR+R++LW+LFSQD   +    V GG    EI  E R     
Sbjct: 121 KNNEGFSGVFEPQRRSCDVRLRSSLWNLFSQDEQRNLPSNVTGG----EIDVEPR----- 180

Query: 181 SSSCLGGPVLEA-EEGESNTNG-EIRVSEEANVVDV-----------VIENGVQEIVE-E 240
             S +  PVLE  +EGE+ ++  E+   EE + V+            ++     EIVE  
Sbjct: 181 -KSSVAEPVLEVNDEGEAESDDEELEEEEEEDYVEAGDFEILNDSGELMREKSDEIVEVR 240

Query: 241 EEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPSGRDLKEIAGSFWSAASVFSKKLQKW 300
           EEIE  + P     +EE + +KD+IDLDS TKKPS      +  SFWSAASVFSKKLQKW
Sbjct: 241 EEIEEAVKPTKGLSEEELKPIKDYIDLDSQTKKPS------VRRSFWSAASVFSKKLQKW 300

Query: 301 RDKQKEKKHRNGG----GSTTLPVEKPIGRHFRETQSEIADYGFGRRSCDTDPRFSLDA- 360
           R  QK KK RNGG    GS  LPVEKPIGR  R+TQSEIADYG+GRRSCDTDPRFSLDA 
Sbjct: 301 RQNQKMKKRRNGGDHRPGSARLPVEKPIGRQLRDTQSEIADYGYGRRSCDTDPRFSLDAG 360

Query: 361 -------------GRMSFDDPRYSFDEPRASWDGYLISRTF-------PRMPTMLSVVED 420
                        GR+S DDPRYSFDEPRASWDG LI RT        P  P+MLSVVED
Sbjct: 361 RFSLDAGRFSVDIGRISLDDPRYSFDEPRASWDGSLIGRTMFPPAARAPPPPSMLSVVED 420

Query: 421 AP----INVFRSDTQIPVEDS------INSTNEEEN---IPGGSSQTRDYYSDSSSRRRK 480
           AP     +V R+D Q PVE+       +N TN   +   IPGGS QTRDYY+DSSSRRRK
Sbjct: 421 APPPVHRHVTRADMQFPVEEPAPPPPVVNQTNGVSDPVIIPGGSIQTRDYYTDSSSRRRK 480

Query: 481 SLDR-SNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRDDC 540
           SLDR S+S+RKTAAAVVA++DE K      VS A +   +   L    RD+N+ ++    
Sbjct: 481 SLDRSSSSMRKTAAAVVADMDEPK----LSVSSAISIDAYSGSL----RDNNNYAVETAD 540

Query: 541 SGSFDSGFDAASVIGTG--NRKEESKKSRRWGKRWNIWGLINRRGGNK-----DEEEDRE 600
           +GSF    + A +IG    N  + +KKSRRWGK W+I GLI R+  NK     +EEEDR 
Sbjct: 541 NGSFR---EPAMMIGDRKVNSNDNNKKSRRWGK-WSILGLIYRKSVNKYEEEEEEEEDRY 600

Query: 601 SRPNG--VERSYSESWPELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKS 643
            R NG  VERS SESWPELR        GG  P+M RSNS+VSWRS              
Sbjct: 601 RRLNGGMVERSLSESWPELRNGGG----GGGGPRMVRSNSNVSWRS-------------- 660

BLAST of Lag0027227 vs. TAIR 10
Match: AT2G38070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 468.0 bits (1203), Expect = 1.2e-131
Identity = 324/658 (49.24%), Postives = 403/658 (61.25%), Query Frame = 0

Query: 9   PAPPLPHRP---CPRHPQEHFTGFCPLCLCERLSLLD---------SSSSRKPPSSSSSS 68
           P PP PHRP   C RHP E FTGFCP CL +RLS+LD         +SSS+KPP    SS
Sbjct: 26  PHPPQPHRPSTSCDRHPDERFTGFCPSCLFDRLSVLDITGKNNNAVASSSKKPP----SS 85

Query: 69  SSALKSIFRPGAASRPSSFFPELRRTKSFSASKNEGFS-GVFEPQRKSCDVRVRNTLWSL 128
           S+ALK+IF+P ++S   SFFPELRRTKSFSASK E FS G FEPQR+SCDVRVRNTLWSL
Sbjct: 86  SAALKAIFKPSSSS--GSFFPELRRTKSFSASKAEAFSLGAFEPQRRSCDVRVRNTLWSL 145

Query: 129 FSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAE---EGESNTNGEIR- 188
           F +D   +S+        G  +     +LE   +S +  PV E E   E E +   +I+ 
Sbjct: 146 FHEDAEHNSQT-----KEGLSVNCSEIDLERI-NSIVKSPVFEEETEIESEQDNEKDIKF 205

Query: 189 --VSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLDSHTKKPSG 248
               E  +V+D        EIVEEEE E     E   ++   +T          T K + 
Sbjct: 206 ETFKEPRSVID--------EIVEEEEEEETKKVEDFTMEFNPQT----------TAKKTN 265

Query: 249 RDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRN---GGGSTTLPVEKPIGRHFRETQS 308
           RD KEIAGSFWSAASVFSKKLQKWR KQK KKHR    G GS+ LPVEK IGR  R+TQS
Sbjct: 266 RDFKEIAGSFWSAASVFSKKLQKWRQKQKLKKHRTGNLGAGSSALPVEKAIGRQLRDTQS 325

Query: 309 EIADYGFGRRSCDTDP-------RFSLDAGRMSFDDPRYSFDEPRASWDGYLISRTFP-- 368
           EIA+YG+GRRSCDTDP       RFSLDAGR+S DDPRYSF+EPRASWDGYLI R     
Sbjct: 326 EIAEYGYGRRSCDTDPRFSIDAGRFSLDAGRVSVDDPRYSFEEPRASWDGYLIGRAAAPM 385

Query: 369 RMPTMLSVVEDAPI--NVFRSDTQIPVEDS--INSTNEEENIPGGSSQTRDYYSD-SSSR 428
           RMP+MLSVVED+P+  +V RSDT IPVE S  ++    +E +PGGS+QTR+YY D SSSR
Sbjct: 386 RMPSMLSVVEDSPVRNHVHRSDTHIPVEKSPQVSEAVIDEIVPGGSAQTREYYLDSSSSR 445

Query: 429 RRKSLDRSNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDSNSNSLRD 488
           RRKSLDRS+S RK +A+V+AEIDE+K   + +                 D  S+SNSLRD
Sbjct: 446 RRKSLDRSSSTRKLSASVMAEIDELKLTQDREAK---------------DLVSHSNSLRD 505

Query: 489 DCSGSFDSGFD--AASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEEDRESRP 548
           DC  S ++ ++      +GT    ++  K  RW   WNI+GL++R+ GNK EEE+R S  
Sbjct: 506 DCC-SVENNYEMGVRENVGTIECNKKRTKKSRWS--WNIFGLLHRKNGNKYEEEERRS-- 565

Query: 549 NGVERSYSESWPELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSSRKSNADSN 608
            GV+R++S SW       N + + GF+PKM RSNSSVSWRS+   GG     ++++ D  
Sbjct: 566 -GVDRTFSGSW-------NVEPRNGFDPKMIRSNSSVSWRSSGTTGG---GLQRNSVD-- 599

Query: 609 GNGNGNGNGNGKKKKDEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGKVKP 629
                 G  +GKKK                +  +NG+L+FYLTP +G RRG      P
Sbjct: 626 ------GYISGKKK---------------VSKAENGMLKFYLTPGKGRRRGSGNSTAP 599

BLAST of Lag0027227 vs. TAIR 10
Match: AT5G01170.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 379.4 bits (973), Expect = 5.7e-105
Identity = 315/697 (45.19%), Postives = 380/697 (54.52%), Query Frame = 0

Query: 3   PIT--DAQPAPPLPHR---PCPRHPQEHFTGFCPLCLCERLSLLD-------SSSSRKPP 62
           P+T  D    P  PHR    C  HP+E F+GFCP CLC+RLS+LD       SSSSRKPP
Sbjct: 9   PVTAVDELAPPSQPHRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPPSSSSRKPP 68

Query: 63  SSSSSSSSALKSIFRPGAASRPSS---------FFPELRRTKSFSASKNEGFSGVFEPQR 122
           S S+ S   LK++F+P ++   +S         FFPELRRTKSFSA  NEGFSG FEPQR
Sbjct: 69  SISAVS---LKALFKPSSSGTNNSNGNGRVRPGFFPELRRTKSFSAKNNEGFSGGFEPQR 128

Query: 123 KSCDVRVRNTLWSLFSQDGSGSSKVVVVGGGGGAEIAAESRNLEETSSSCLGGPVLEAEE 182
           +SCDVR+R+   +L   + +   K+                  EE   S +   VLE  E
Sbjct: 129 RSCDVRLRDDERNLPINEAASVDKIE-----------------EEARESSVSEIVLEVTE 188

Query: 183 GESNTNGEIRVSEEANVVDVVIENGVQEIVEEEEIEAELGPESVQLQEEFRTMKDHIDLD 242
                  EI   EE        E    EIVEE+   +E+G E    +EE + MKD++DL 
Sbjct: 189 -----EAEIEEDEENG------EKDPGEIVEEK--SSEIGEE----EEELKPMKDYMDLY 248

Query: 243 SHTKKPSGRDLKEIAGSFWSAASVFSKKLQKWRDKQKEKKHRNGGGSTTLPVEKPIGRHF 302
           S TKKPS   +K+ AGSF+SAASVFSKKLQKW+ KQK KK RNG G          GR  
Sbjct: 249 SQTKKPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGG---------GR-- 308

Query: 303 RETQSEIADYGFGRRSCDTDPRFSLDA-------GRMSFDDPRYSFDEPRASWDGYLISR 362
              QSEI   G GRRS DTDPRFSLDA       GR+S DD RYS DEPRASWDG+LI R
Sbjct: 309 --PQSEI---GVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSLDEPRASWDGHLIGR 368

Query: 363 T----FPRMPTMLSVVEDAPINVFRSDTQIPVEDSINSTNEEEN----IPGGSSQTRDYY 422
           T     P  P+MLSVVE+AP+N  RSD QIP   SI   + + +    IPGGS+QTRDYY
Sbjct: 369 TTAARVPLPPSMLSVVENAPLN--RSDMQIPSSPSIKPISGDSDPIIIIPGGSNQTRDYY 428

Query: 423 S-DSSSRRRKSLDRSNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDICHGPKLGIPDRDS 482
           +   SSRRRKSLDRSNSIRK    +V E++++KSVSN     +TT I           DS
Sbjct: 429 TGPPSSRRRKSLDRSNSIRK----IVTELEDVKSVSN-----STTTI-----------DS 488

Query: 483 NSNSLRDDCSGSFDSGFDAASVIGTGNRKEESKKSRRWGKRWNIWGLINRRGGNKDEEED 542
           NS    ++                 GN+  + KKSRRWGK W+I G I R+ G  DEEED
Sbjct: 489 NSMETAEN----------------KGNQNGD-KKSRRWGK-WSILGFIYRK-GKDDEEED 548

Query: 543 RESRPNG---VERSYSESWPELRGERNSDVKGGFNPKMFRSNSSVSWRSASMIGGTFSSS 602
           R SR N    VERS SESWPE+R         G  PKM RSNS+VSWRS           
Sbjct: 549 RYSRSNSAGMVERSLSESWPEMRNGE------GGGPKMRRSNSNVSWRS----------- 568

Query: 603 RKSNADSNGNGNGNGNGNGKKKKDEPVLERNRSARHSPTNVDNGLLRFYLTPLR------ 643
                        +G G+           RN+S+R+S  + +NG+LRFYLTP+R      
Sbjct: 609 -------------SGGGSA----------RNKSSRYSSKDGENGMLRFYLTPMRRSWKTS 568

BLAST of Lag0027227 vs. TAIR 10
Match: AT5G58930.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 176.4 bits (446), Expect = 7.3e-44
Identity = 196/646 (30.34%), Postives = 291/646 (45.05%), Query Frame = 0

Query: 18  CPRHP-QEHFTGFCPLCLCERLSLLDSSSSRKPPSSSSSSSSALKSIFRPGAASRPSSFF 77
           C RHP  +  TGFC  CL ERLS +++       SSS S+S+                  
Sbjct: 17  CHRHPSSKPTTGFCATCLRERLSTIEAL------SSSVSAST------------------ 76

Query: 78  PELRRTKSFSASKNEGFSGVFEPQRKSCDVRVRNTLWSLFSQDGSGSSKVVVVGGGGGAE 137
            ELRR +S+S  ++   S + +P+R+SCDVR  +                          
Sbjct: 77  -ELRRVRSYSV-RDASASVLDQPRRRSCDVRSNH-------------------------- 136

Query: 138 IAAESRNLEETSSSCLGGPVLEAEEGESNTNGEIRVSEEANVVDVVIENGVQEIVEEEEI 197
              +  + +E   S +  P+                              V +++E+EE 
Sbjct: 137 ---DDDDDDELLKSSIRFPI------------------------------VPDLIEDEEE 196

Query: 198 EAELGPESVQLQ---EEFRTMKDHIDLDSHTK--KPSGRDLKEIAGSFWSAASVFSKKLQ 257
           E + G + V+ +    E +TMK+ IDL+S  +  K +G+D            SVFS+ L+
Sbjct: 197 EDDEGKKLVEEEIEDGEQKTMKELIDLESRNQQLKNNGKD------------SVFSRTLR 256

Query: 258 KWRDKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEIADYGFGRRSCDTDPRFSLDAGRM 317
           K+  K   K   +G                            GRRSCD DPR SLDAGR+
Sbjct: 257 KFSLKHHRKIPDSGN-------------------------SLGRRSCDVDPRLSLDAGRV 316

Query: 318 SFDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDAPINVFRSDTQIPVEDSINSTNE 377
                  SFDEPRASWDG LI +T+P++  + SV ED   +  +   +   ED       
Sbjct: 317 -------SFDEPRASWDGCLIGKTYPKLIPLSSVTEDVKASPEKITGEKVEED------- 376

Query: 378 EENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAAVVAEIDEMKSVSNAKVSPATTD 437
           E+N PGG++QTRDYY D  SRRR+S DRS      +   + E+DE+K++SNAKVSP T  
Sbjct: 377 EKNNPGGTAQTRDYYLD--SRRRRSFDRS------SRHGLLEVDELKAISNAKVSPETVG 436

Query: 438 ICHGPKLGIPD---RDSNSNSLRDDCSGSFDSGFDAASVIGTGNRKEE-----SKKSRRW 497
           + HG KL + +   RDSN  S+++    S + G      +  G  K++      K  + W
Sbjct: 437 LFHGAKLLVTERELRDSNWYSIKNYKPESLELGSKGVGCVAAGEVKKQDGFGLKKSGKNW 496

Query: 498 GKRWNIWGLINRRGG-NKDEEEDRESRP---NGVERSYSESWPELRGERNSDVKGGFNPK 557
            K WN WGLI R+    K+E +  +S     N +E S +ES  +LR     +  G  + K
Sbjct: 497 SKGWNFWGLIQRKTDVAKNEMKTEQSLKLGGNTMEGSLAESLLKLRRVAKGETNGDVSEK 516

Query: 558 MFRSNSSVS-------WRSASMIGGTFSSSRKSNADSNGNGNGNGNGNGKK--------- 617
           + RS S  +        R AS++ G F   R S+ D   +G+  G   G++         
Sbjct: 557 LIRSYSVSARKSCDGMLRGASIVNG-FEGGR-SSCDGLFHGSITGVETGRRSLCEDGMFH 516

Query: 618 ----KKDEPVLERNRSARHSPTNVDNGLLRFYLTPLRGSRRGGSGK 626
               K++  +   ++   +SP N+ NG++RFYLTPL       SGK
Sbjct: 617 GVEGKRNHLLQSDDKLGTYSPDNLRNGMVRFYLTPLNSHMTSKSGK 516

BLAST of Lag0027227 vs. TAIR 10
Match: AT3G46990.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 173.3 bits (438), Expect = 6.2e-43
Identity = 198/642 (30.84%), Postives = 305/642 (47.51%), Query Frame = 0

Query: 18  CPRHPQEHFT-GFCPLCLCERLSLLDSSSSRKPPSSSSSSSSALKSIFRPGAASRPSSFF 77
           C RHP    T GFC  CL ERL  ++         + SSS +A+++              
Sbjct: 16  CHRHPSAKPTSGFCASCLRERLVTIE---------AQSSSLAAVQT-------------- 75

Query: 78  PELRRTKSFSASKNEGFSGVFEPQRKSCDVR-VRNTLWSLFSQDGSGSSKVVVVGGGGGA 137
           PELRR +S+S  +N   S   +P+R+SCDVR   ++L  LF  D                
Sbjct: 76  PELRRIRSYSV-RNASVSVSDQPRRRSCDVRSSASSLLDLFVDDD--------------- 135

Query: 138 EIAAESRNLEETSSSCLGGPVLEAEEGESNTNGEIRVSEEANVVDVVIENGVQEIVEEEE 197
                    EE   S +  P++           +++  EE        E   ++  + E+
Sbjct: 136 ---------EERVDSSIRKPLVP----------DLKEEEEE-------EEEEEDYYDGED 195

Query: 198 IEAELGPESVQLQEEFRTMKDHIDLD--SHTKKPSGRDLKEIAGSFWSAASVFSKKLQKW 257
           I+     +  ++ EE +TMK+ IDLD  +  KK +G+DLKEI       ASV S++L+ +
Sbjct: 196 IKGFDEGKPRKIVEENKTMKEFIDLDWRNQIKKNNGKDLKEI-------ASVLSRRLKNF 255

Query: 258 R-DKQKEKKHRNGGGSTTLPVEKPIGRHFRETQSEIADYGFGRRSCDTDPRFSLDAGRMS 317
             +K+ ++K                      + S  A    GR S D DPR S D GR+ 
Sbjct: 256 TLNKRNDEK----------------------SDSRFAGIVNGRHSSDVDPRLSFDGGRI- 315

Query: 318 FDDPRYSFDEPRASWDGYLISRTFPRMPTMLSVVEDAPINVFRSDTQIPVEDSINSTNEE 377
                 SF++PR+SWDG LI +++ ++ T+ +V EDA         +  VE+      E+
Sbjct: 316 ------SFEKPRSSWDGCLIEKSYHKLTTLSTVTEDA-------KAKCGVEE--EEVEEK 375

Query: 378 ENIPGGSSQTRDYYSDSSSRRRKSLDRSNSIRKTAAAVVAEIDEMKSVSNAKVSPATTDI 437
           E  PGG+ QT++YYSD  SRRR+S DRS SI++     + E+DE++ +SNAKVSP T  +
Sbjct: 376 EKSPGGTVQTKNYYSD--SRRRRSFDRSVSIKRQG---LLEVDELRGISNAKVSPETVGL 435

Query: 438 CHGPKLGIPD---RDSNSNSLRD---DCSGSFDSGFDAASVIGTGNRKE--ESKKSR-RW 497
            HG KL + +   RDSN  S+++   +       G    +  G G +++  E KK R +W
Sbjct: 436 FHGAKLLVTEKELRDSNWYSIKNVKPESKELVSKGKICIAAGGEGKKQDSVELKKPRKKW 495

Query: 498 GKRWNIWGLINRRGGNKDE---EEDRESRPNGVERSYSESWPELRGERNSDVKGGFNPKM 557
            K WNIWGLI R+   K+E   E+  +   N VE S +ES  +LR     +   G + K+
Sbjct: 496 PKGWNIWGLIQRKNEAKNEIKTEQILKLEGNAVEGSLAESLLKLRRVGKGETNVGVSEKL 542

Query: 558 FRS------NSSVSWRSASMIGGTFSSSRKS---------NADSNGNGNGNGNGNG-KKK 617
            +S       S    RS + I   F   R S         N+   G  + +G  NG + K
Sbjct: 556 LKSYSVSARKSCDGVRSGANIVSGFEGGRSSCDGLFHGSINSVEAGRNSCDGLVNGIEGK 542

Query: 618 KDEPVLERNRS-ARHSPTNVDNGLLRFYLTPLRGSRRGGSGK 626
           ++  +L+RN +    S  N++  + RFYL+P++  +   SGK
Sbjct: 616 QNHHLLQRNANVGTCSQENLEKSMFRFYLSPVKSHKTSKSGK 542