Lag0027163 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027163
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Laccase
Location	chr10: 45543224 .. 45543619 (-)
RNA-Seq Expression	Lag0027163
Synteny	Lag0027163

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAGCAGCGGAACGAAGGTGGCGGTGGTGCCGTACAACGCCGCCGTGGAAGTAGTAATGCAGGACACGAGCATCGTCACGGCGGAGAGTCACCCGCTCCACCTCCACGGCTTCAATTTCTTCGTGATGGGTCAAGGCATCGGAAACTTCGACCCGAACAAAGACCCGGCCAAGTTCAACCTCGCCGACCCGGCGGAGAGGAACACCGTCGGCGTCCCTTCCGGCGGCTGGGTGGCGATCCGGTTCATCGCCGACAATCCGGGAGCTTGGTTTATGCACTGTCACTTGGAAGTCCACACCAGTTGGGGGCTGAAAATGGCGTGGATCGTTCAGGACGGCAAACGGCCCCATCAGAAGCTGCCGCCGCCGCCTTCCGATCTGCCCAAATGCTAA

mRNA sequence

ATGGTGAGCAGCGGAACGAAGGTGGCGGTGGTGCCGTACAACGCCGCCGTGGAAGTAGTAATGCAGGACACGAGCATCGTCACGGCGGAGAGTCACCCGCTCCACCTCCACGGCTTCAATTTCTTCGTGATGGGTCAAGGCATCGGAAACTTCGACCCGAACAAAGACCCGGCCAAGTTCAACCTCGCCGACCCGGCGGAGAGGAACACCGTCGGCGTCCCTTCCGGCGGCTGGGTGGCGATCCGGTTCATCGCCGACAATCCGGGAGCTTGGTTTATGCACTGTCACTTGGAAGTCCACACCAGTTGGGGGCTGAAAATGGCGTGGATCGTTCAGGACGGCAAACGGCCCCATCAGAAGCTGCCGCCGCCGCCTTCCGATCTGCCCAAATGCTAA

Coding sequence (CDS)

ATGGTGAGCAGCGGAACGAAGGTGGCGGTGGTGCCGTACAACGCCGCCGTGGAAGTAGTAATGCAGGACACGAGCATCGTCACGGCGGAGAGTCACCCGCTCCACCTCCACGGCTTCAATTTCTTCGTGATGGGTCAAGGCATCGGAAACTTCGACCCGAACAAAGACCCGGCCAAGTTCAACCTCGCCGACCCGGCGGAGAGGAACACCGTCGGCGTCCCTTCCGGCGGCTGGGTGGCGATCCGGTTCATCGCCGACAATCCGGGAGCTTGGTTTATGCACTGTCACTTGGAAGTCCACACCAGTTGGGGGCTGAAAATGGCGTGGATCGTTCAGGACGGCAAACGGCCCCATCAGAAGCTGCCGCCGCCGCCTTCCGATCTGCCCAAATGCTAA

Protein sequence

MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKFNLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQKLPPPPSDLPKC

Homology

[Close]

BLAST of Lag0027163 vs. NCBI nr
Match: XP_022983919.1 (laccase-17-like [Cucurbita maxima])

HSP 1 Score: 276.9 bits (707), Expect = 8.7e-71
Identity = 126/131 (96.18%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
           MVSSGTKVAV+PYNAAVE+VMQDTSIVTAESHPLHLHGFNFFV+GQGIGNFDPNKDPAKF
Sbjct: 446 MVSSGTKVAVLPYNAAVELVMQDTSIVTAESHPLHLHGFNFFVVGQGIGNFDPNKDPAKF 505

Query: 61  NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
           NLADPAERNTVGVPSGGWVAIRF+ADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRP+QK
Sbjct: 506 NLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPNQK 565

Query: 121 LPPPPSDLPKC 132
           LPPPPSDLPKC
Sbjct: 566 LPPPPSDLPKC 576

[Close]

BLAST of Lag0027163 vs. NCBI nr
Match: XP_023528616.1 (laccase-17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 275.4 bits (703), Expect = 2.5e-70
Identity = 125/131 (95.42%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
           MVSSGTKV V+PYNAAVE+VMQDTSIVTAESHPLHLHGFNFFV+GQGIGNFDPNKDPAKF
Sbjct: 446 MVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLHLHGFNFFVVGQGIGNFDPNKDPAKF 505

Query: 61  NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
           NLADPAERNTVGVPSGGWVAIRF+ADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRP+QK
Sbjct: 506 NLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPNQK 565

Query: 121 LPPPPSDLPKC 132
           LPPPPSDLPKC
Sbjct: 566 LPPPPSDLPKC 576

[Close]

BLAST of Lag0027163 vs. NCBI nr
Match: KAG7017284.1 (Laccase-17 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 275.4 bits (703), Expect = 2.5e-70
Identity = 125/131 (95.42%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
           MVSSGTKV V+PYNAAVE+VMQDTSIVTAESHPLHLHGFNFFV+GQGIGNFDPNKDPAKF
Sbjct: 446 MVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLHLHGFNFFVVGQGIGNFDPNKDPAKF 505

Query: 61  NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
           NLADPAERNTVGVPSGGWVAIRF+ADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRP+QK
Sbjct: 506 NLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPNQK 565

Query: 121 LPPPPSDLPKC 132
           LPPPPSDLPKC
Sbjct: 566 LPPPPSDLPKC 576

[Close]

BLAST of Lag0027163 vs. NCBI nr
Match: XP_022934632.1 (uncharacterized protein LOC111441767 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 2.5e-70
Identity = 125/131 (95.42%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1    MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
            MVSSGTKV V+PYNAAVE+VMQDTSIVTAESHPLHLHGFNFFV+GQGIGNFDPNKDPAKF
Sbjct: 1104 MVSSGTKVVVLPYNAAVELVMQDTSIVTAESHPLHLHGFNFFVVGQGIGNFDPNKDPAKF 1163

Query: 61   NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
            NLADPAERNTVGVPSGGWVAIRF+ADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRP+QK
Sbjct: 1164 NLADPAERNTVGVPSGGWVAIRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPNQK 1223

Query: 121  LPPPPSDLPKC 132
            LPPPPSDLPKC
Sbjct: 1224 LPPPPSDLPKC 1234

[Close]

BLAST of Lag0027163 vs. NCBI nr
Match: XP_038905310.1 (laccase-17-like [Benincasa hispida])

HSP 1 Score: 273.9 bits (699), Expect = 7.4e-70
Identity = 123/131 (93.89%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
           MVSSGTKVAV+PYN AVE+VMQDTSIVTAESHPLHLHGFNFFV+GQGIGNFDPNKDPA+F
Sbjct: 466 MVSSGTKVAVLPYNTAVELVMQDTSIVTAESHPLHLHGFNFFVVGQGIGNFDPNKDPARF 525

Query: 61  NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
           NLADPAERNT+GVPSGGWVAIRF+ADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRP+QK
Sbjct: 526 NLADPAERNTIGVPSGGWVAIRFVADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPNQK 585

Query: 121 LPPPPSDLPKC 132
           LPPPPSDLPKC
Sbjct: 586 LPPPPSDLPKC 596

[Close]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot
Match: Q9FJD5 (Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 5.9e-62
Identity = 104/131 (79.39%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MVSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKF 60
           MVS+GT + V+PYN +VE+VMQDTSI+ AESHPLHLHGFNFFV+GQG GNFDPNKDP  F
Sbjct: 447 MVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNF 506

Query: 61  NLADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQK 120
           NL DP ERNTVGVPSGGW AIRF+ADNPG WFMHCHLEVHTSWGL+MAW+V DG +P QK
Sbjct: 507 NLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWGLRMAWLVLDGDKPDQK 566

Query: 121 LPPPPSDLPKC 132
           L PPP+DLPKC
Sbjct: 567 LLPPPADLPKC 577

[Close]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot
Match: B9FJH4 (Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 5.0e-61
Identity = 106/130 (81.54%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 2   VSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKFN 61
           V +GTKV V+PY A VE+VMQDTSI+ AESHPLHLHGFNFFV+GQG GNFDP  DPAKFN
Sbjct: 445 VMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPINDPAKFN 504

Query: 62  LADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQKL 121
           L DP ERNTVGVP+GGWVAIRF ADNPG WFMHCHLEVH SWGLKMAW+V DG RP QKL
Sbjct: 505 LYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLVLDGSRPDQKL 564

Query: 122 PPPPSDLPKC 132
           PPPP DLPKC
Sbjct: 565 PPPPLDLPKC 574

[Close]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot
Match: P0DKK6 (Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.0e-61
Identity = 106/130 (81.54%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 2   VSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKFN 61
           V +GTKV V+PY A VE+VMQDTSI+ AESHPLHLHGFNFFV+GQG GNFDP  DPAKFN
Sbjct: 445 VMNGTKVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPINDPAKFN 504

Query: 62  LADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQKL 121
           L DP ERNTVGVP+GGWVAIRF ADNPG WFMHCHLEVH SWGLKMAW+V DG RP QKL
Sbjct: 505 LYDPVERNTVGVPAGGWVAIRFHADNPGVWFMHCHLEVHMSWGLKMAWLVLDGSRPDQKL 564

Query: 122 PPPPSDLPKC 132
           PPPP DLPKC
Sbjct: 565 PPPPLDLPKC 574

[Close]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot
Match: Q10ND7 (Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 3.2e-60
Identity = 101/130 (77.69%), Postives = 116/130 (89.23%), Query Frame = 0


Query: 2   VSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKFN 61
           VS+GT+V V+PYNA+VEVV+QDTSI+ AESHPLHLHGF+FFV+GQG GN+DP+K PA+FN
Sbjct: 449 VSNGTRVVVLPYNASVEVVLQDTSILGAESHPLHLHGFDFFVVGQGTGNYDPSKHPAEFN 508

Query: 62  LADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQKL 121
           L DP +RNTVGVP+GGWVAIRF ADNPG WFMHCHLEVHT+WGLKMAW+V DG  P QKL
Sbjct: 509 LVDPVQRNTVGVPAGGWVAIRFFADNPGVWFMHCHLEVHTTWGLKMAWVVNDGPLPEQKL 568

Query: 122 PPPPSDLPKC 132
            PPPSDLP C
Sbjct: 569 MPPPSDLPMC 578

[Close]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot
Match: Q5N9X2 (Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 5.2e-58
Identity = 98/130 (75.38%), Postives = 112/130 (86.15%), Query Frame = 0


Query: 2   VSSGTKVAVVPYNAAVEVVMQDTSIVTAESHPLHLHGFNFFVMGQGIGNFDPNKDPAKFN 61
           V +GTK+ V+ YN +VE+VMQDTSI+  ESHPLHLHGFNFFV+GQG GN+D   DPAKFN
Sbjct: 450 VKTGTKLLVLRYNTSVELVMQDTSILGIESHPLHLHGFNFFVIGQGFGNYDAVNDPAKFN 509

Query: 62  LADPAERNTVGVPSGGWVAIRFIADNPGAWFMHCHLEVHTSWGLKMAWIVQDGKRPHQKL 121
           L DP ERNTVGVP+GGWVAIRF+ADNPG WFMHCHLE HT+WGL+MAW+V DG  P+QKL
Sbjct: 510 LVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEAHTTWGLRMAWLVLDGSHPNQKL 569

Query: 122 PPPPSDLPKC 132
            PPPSDLPKC
Sbjct: 570 LPPPSDLPKC 579

The following BLAST results are available for this feature:

BLAST of Lag0027163 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983919.1	8.7e-71	96.18	laccase-17-like [Cucurbita maxima]	[more]
XP_023528616.1	2.5e-70	95.42	laccase-17-like [Cucurbita pepo subsp. pepo]	[more]
KAG7017284.1	2.5e-70	95.42	Laccase-17 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022934632.1	2.5e-70	95.42	uncharacterized protein LOC111441767 [Cucurbita moschata]	[more]
XP_038905310.1	7.4e-70	93.89	laccase-17-like [Benincasa hispida]	[more]

BLAST of Lag0027163 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJD5	5.9e-62	79.39	Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1	[more]
B9FJH4	5.0e-61	81.54	Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2	[more]
P0DKK6	5.0e-61	81.54	Laccase-13 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC13 PE=2 SV=1	[more]
Q10ND7	3.2e-60	77.69	Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1	[more]
Q5N9X2	5.2e-58	75.38	Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 4..114 e-value: 7.4E-39 score: 132.6
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 1..131 e-value: 3.4E-49 score: 169.4
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 8..131
None	No IPR available	PANTHER	PTHR11709:SF342	LACCASE-10	coord: 1..131
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 1..131
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 89..109
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 94..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027163.1	Lag0027163.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0048046	apoplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity
molecular_function	GO:0016491	oxidoreductase activity