Lag0027149 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027149
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	metallothionein-like protein type 2
Location	chr10: 45429094 .. 45429727 (+)
RNA-Seq Expression	Lag0027149
Synteny	Lag0027149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTGCTGTGGTGGAAACTGTGGATGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGGTGAGTAATCGCTAATTTTCTCCTTTGAATTTGATCGTGTGAATCGAATTTAATTTTTTATTTCTGTAGAAAATGATTTCTCGAGAATCTGATCTTCCGTTTGCTTCTATGTAGCTTTCGATTTCGTTTTCGTCTCTGTATCTTTGTTCATCGCTCGATTGCGATCTGATCATGTGCTGATTTTCCTTACTGAATTTTGAGTTTGAGAAATGATCGCGCGATCGCGGCCTATATTCTTCTGAAATCTCTGGAAATGACTTGGATAATCGTTTTTAGAGGATAATCGCGATGATTCTGACGTTTTGGTCTTTCCTTATGCAGGTGCAAGATGTTCCCTGACATGAGCTTCTCTGAGACCACCGCCACCATCGGGACCTTCGTCGTCGGTCTCGCTCCTCAGAAGATGTAAGGTTTCGAAATTACTCTATCATTCAAGAGAAATTTAGTCATGATTTTAATTAACGGTTCTGGTTTTCCACATCAGGTCCTTCGAGGTGGCAGAGATGGGAGCCGAGAGCGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

mRNA sequence

ATGTCGTGCTGTGGTGGAAACTGTGGATGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGGTGCAAGATGTTCCCTGACATGAGCTTCTCTGAGACCACCGCCACCATCGGGACCTTCGTCGTCGGTCTCGCTCCTCAGAAGATGTCCTTCGAGGTGGCAGAGATGGGAGCCGAGAGCGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

Coding sequence (CDS)

ATGTCGTGCTGTGGTGGAAACTGTGGATGCGGCTCTGCCTGCAAGTGCGGAAGTGGCTGTGGAGGGTGCAAGATGTTCCCTGACATGAGCTTCTCTGAGACCACCGCCACCATCGGGACCTTCGTCGTCGGTCTCGCTCCTCAGAAGATGTCCTTCGAGGTGGCAGAGATGGGAGCCGAGAGCGGCTGCAAGTGCGGTGACAACTGCACCTGCGATCCATGCAACTGTAAATGA

Protein sequence

MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAESGCKCGDNCTCDPCNCK

Homology

[Close]

BLAST of Lag0027149 vs. NCBI nr
Match: XP_022949247.1 (metallothionein-like protein type 2 [Cucurbita moschata] >XP_022971579.1 metallothionein-like protein type 2 [Cucurbita maxima] >XP_023538679.1 metallothionein-like protein type 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 161.4 bits (407), Expect = 3.1e-36
Identity = 74/77 (96.10%), Postives = 76/77 (98.70%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATI TFVVGLAPQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 SGCKCGDNCTCDPCNCK 78
          +GCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

[Close]

BLAST of Lag0027149 vs. NCBI nr
Match: KAG6596409.1 (Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027952.1 Metallothionein-like protein type 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 159.8 bits (403), Expect = 9.1e-36
Identity = 73/77 (94.81%), Postives = 76/77 (98.70%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPD+SFSETTATI TFVVGLAPQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDVSFSETTATIETFVVGLAPQKMSFEVAEMGAE 60

Query: 61 SGCKCGDNCTCDPCNCK 78
          +GCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

[Close]

BLAST of Lag0027149 vs. NCBI nr
Match: XP_038906044.1 (metallothionein-like protein type 2 [Benincasa hispida])

HSP 1 Score: 159.5 bits (402), Expect = 1.2e-35
Identity = 73/77 (94.81%), Postives = 74/77 (96.10%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGS CKCGSGCGGCKMFPDMSFSETTATI TFVVG APQKMSFEVAEMGAE
Sbjct: 1  MSCCGGNCGCGSGCKCGSGCGGCKMFPDMSFSETTATIETFVVGFAPQKMSFEVAEMGAE 60

Query: 61 SGCKCGDNCTCDPCNCK 78
          +GCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

[Close]

BLAST of Lag0027149 vs. NCBI nr
Match: XP_022934858.1 (metallothionein-like protein type 2 B [Cucurbita moschata] >XP_023529118.1 metallothionein-like protein type 2 B [Cucurbita pepo subsp. pepo] >KAG6580545.1 Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017297.1 Metallothionein-like protein type 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 158.7 bits (400), Expect = 2.0e-35
Identity = 72/77 (93.51%), Postives = 75/77 (97.40%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATI TFVVGLAPQKMSFE +EMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIETFVVGLAPQKMSFEASEMGAE 60

Query: 61 SGCKCGDNCTCDPCNCK 78
          +GCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

[Close]

BLAST of Lag0027149 vs. NCBI nr
Match: XP_022983596.1 (metallothionein-like protein type 2 B [Cucurbita maxima])

HSP 1 Score: 156.4 bits (394), Expect = 1.0e-34
Identity = 71/77 (92.21%), Postives = 74/77 (96.10%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKMFPDMSFSETTATI  FVVGLAPQKMSFE +EMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMFPDMSFSETTATIEIFVVGLAPQKMSFEASEMGAE 60

Query: 61 SGCKCGDNCTCDPCNCK 78
          +GCKCGDNCTCDPCNCK
Sbjct: 61 NGCKCGDNCTCDPCNCK 77

[Close]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot
Match: P30564 (Metallothionein-like protein type 2 OS=Ricinus communis OX=3988 GN=MTI PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 6.8e-26
Identity = 56/81 (69.14%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGA- 60
          MSCCGGNCGCGS CKCG+GCGGCKM+PDMSFSE T T  T V+G+  +K  FE  EMG  
Sbjct: 1  MSCCGGNCGCGSGCKCGNGCGGCKMYPDMSFSEKTTT-ETLVLGVGAEKAHFEGGEMGVV 60

Query: 61 ---ESGCKCGDNCTCDPCNCK 78
             E GCKCGDNCTC+PC CK
Sbjct: 61 GAEEGGCKCGDNCTCNPCTCK 80

[Close]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot
Match: Q40158 (Metallothionein-like protein type 2 B OS=Solanum lycopersicum OX=4081 GN=MTB PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.6e-25
Identity = 51/80 (63.75%), Postives = 64/80 (80.00%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMG-- 60
          MSCCGGNCGCGS+CKCG+GCGGCKM+PDMS++E++ T  T V+G+ P+K SF   EMG  
Sbjct: 1  MSCCGGNCGCGSSCKCGNGCGGCKMYPDMSYTESSTTTETLVLGVGPEKTSFGAMEMGES 60

Query: 61 --AESGCKCGDNCTCDPCNC 77
            AE+GCKCG +C C+PC C
Sbjct: 61 PVAENGCKCGSDCKCNPCTC 80

[Close]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot
Match: P43398 (Metallothionein-like protein 2 OS=Trifolium repens OX=3899 GN=MT1A PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.4e-25
Identity = 56/78 (71.79%), Postives = 63/78 (80.77%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMGAE 60
          MSCCGGNCGCGSACKCG+GCGGCKM  D+S++E+T T  T V+G+   K  FE AEMGAE
Sbjct: 1  MSCCGGNCGCGSACKCGNGCGGCKMNADLSYTESTTT-ETIVMGVGSAKAQFEGAEMGAE 60

Query: 61 S-GCKCGDNCTCDPCNCK 78
          S GCKCG NCTCDPC CK
Sbjct: 61 SGGCKCGANCTCDPCTCK 77

[Close]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot
Match: P43390 (Metallothionein-like protein type 2 OS=Actinidia deliciosa OX=3627 GN=pKIWI504 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.8e-25
Identity = 54/79 (68.35%), Postives = 63/79 (79.75%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMG-- 60
          MSCCGG CGCGS+C CGSGCGGC M+PD+S+SE T T  T +VG+APQK  FE +EMG  
Sbjct: 1  MSCCGGKCGCGSSCSCGSGCGGCGMYPDLSYSEMTTT-ETLIVGVAPQKTYFEGSEMGVA 60

Query: 61 AESGCKCGDNCTCDPCNCK 78
          AE+GCKCG +C CDPC CK
Sbjct: 61 AENGCKCGSDCKCDPCTCK 78

[Close]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot
Match: Q39459 (Metallothionein-like protein 2 OS=Cicer arietinum OX=3827 PE=3 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 7.5e-25
Identity = 55/80 (68.75%), Postives = 62/80 (77.50%), Query Frame = 0


Query: 1  MSCCGGNCGCGSACKCGSGCGGCKMFPDMSFSETTATIGTFVVGLAPQKMSFEVAEMG-- 60
          MSCCGGNCGCGS+CKCGSGCGGCKM+PDMS++E T T  T V+G+A  K  FE AEMG  
Sbjct: 1  MSCCGGNCGCGSSCKCGSGCGGCKMYPDMSYTEQT-TSETLVMGVASGKTQFEGAEMGFG 60

Query: 61 -AESGCKCGDNCTCDPCNCK 78
              GCKCG NCTC+PC CK
Sbjct: 61 AENDGCKCGSNCTCNPCTCK 79

The following BLAST results are available for this feature:

BLAST of Lag0027149 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949247.1	3.1e-36	96.10	metallothionein-like protein type 2 [Cucurbita moschata] >XP_022971579.1 metallo...	[more]
KAG6596409.1	9.1e-36	94.81	Metallothionein-like protein type 2, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_038906044.1	1.2e-35	94.81	metallothionein-like protein type 2 [Benincasa hispida]	[more]
XP_022934858.1	2.0e-35	93.51	metallothionein-like protein type 2 B [Cucurbita moschata] >XP_023529118.1 metal...	[more]
XP_022983596.1	1.0e-34	92.21	metallothionein-like protein type 2 B [Cucurbita maxima]	[more]

BLAST of Lag0027149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30564	6.8e-26	69.14	Metallothionein-like protein type 2 OS=Ricinus communis OX=3988 GN=MTI PE=3 SV=1	[more]
Q40158	2.6e-25	63.75	Metallothionein-like protein type 2 B OS=Solanum lycopersicum OX=4081 GN=MTB PE=...	[more]
P43398	4.4e-25	71.79	Metallothionein-like protein 2 OS=Trifolium repens OX=3899 GN=MT1A PE=3 SV=1	[more]
P43390	5.8e-25	68.35	Metallothionein-like protein type 2 OS=Actinidia deliciosa OX=3627 GN=pKIWI504 P...	[more]
Q39459	7.5e-25	68.75	Metallothionein-like protein 2 OS=Cicer arietinum OX=3827 PE=3 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000347	Metallothionein, family 15, plant	PFAM	PF01439	Metallothio_2	coord: 1..76 e-value: 2.9E-28 score: 98.6
IPR000347	Metallothionein, family 15, plant	PANTHER	PTHR33543	METALLOTHIONEIN-LIKE PROTEIN 2A	coord: 1..76
None	No IPR available	PANTHER	PTHR33543:SF1	METALLOTHIONEIN-LIKE PROTEIN 2A	coord: 1..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027149.1	Lag0027149.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding