Lag0027136 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027136
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Actin-depolymerizing factor
Location	chr10: 45326399 .. 45327295 (+)
RNA-Seq Expression	Lag0027136
Synteny	Lag0027136

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGGAAAATCCCATCCTGCCTGTATGGCTATATCCCCAATCTTGCGTGGCTATGGAAGAGAATCCATACTATTTACTTGTTACATGATCGTGGTCTTTTGACTAATTACGACCAAAAATGGATGGCTAAGATGCAGTTCTCTCGGGAGAAATAGAACAATTGTTGAAATTTTGCTAAATCTGATCAGATCAGAAAATTCCCCAACTTTCTGCCTTTCCAGTGGTAGATTAATCAATACTCAACCCGTTGCTTTGCTTTGCAGCGTTTGGTTTTATAGCTGTGTTATCAAGAACGTTTTGTTTTTTCAATTTTGTTCTGCTTTCTCGACTTGTTCTGTATGCGTCTGTTCTGGACTTCAAAATTATATTAAGCCGTTTCGTTCTGCAATACATAGGCCAATGCAGCATCGGGAATTGCCGTGAATGATGATTGCAAGCTGAAGTTTTTGGAGCTCAAGGCAAAAAGAACCTATCGTTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTTGGCGAACCAACTCAAAGTTATGAGGACTTTGCTGCTAGCCTCCCTTCTGACGAATGTCGTTACGCCATCTATGATTTCGACTTTGTTACTGAAGAGAACTGTCAAAAGAGTAGAATCTTCTTCATTGCCTGGTATAGCTGACATCTCCTTTACTACATTCCTTTCATGTTTATCTTTCCAACTATCGCGCTTACAAAATCGCCATTGTTTGAAGGTCCCCTGATAATTCAAGAGTTAGAAGCAAGATGATATATGCCAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGATTCCAGGTTGAGCTGCAAGCTACGGATCCGACTGAGATGGGTCTCGACGTCATTCGAAGCCGAGCTGGCTGA

mRNA sequence

ATGCGGGAAAATCCCATCCTGCCTGTATGGCTATATCCCCAATCTTGCGTGGCTATGGAAGAGAATCCATACTATTTACTTGCCAATGCAGCATCGGGAATTGCCGTGAATGATGATTGCAAGCTGAAGTTTTTGGAGCTCAAGGCAAAAAGAACCTATCGTTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTTGGCGAACCAACTCAAAGTTATGAGGACTTTGCTGCTAGCCTCCCTTCTGACGAATGTCGTTACGCCATCTATGATTTCGACTTTGTTACTGAAGAGAACTGTCAAAAGAGTAGAATCTTCTTCATTGCCTGGTCCCCTGATAATTCAAGAGTTAGAAGCAAGATGATATATGCCAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGATTCCAGGTTGAGCTGCAAGCTACGGATCCGACTGAGATGGGTCTCGACGTCATTCGAAGCCGAGCTGGCTGA

Coding sequence (CDS)

ATGCGGGAAAATCCCATCCTGCCTGTATGGCTATATCCCCAATCTTGCGTGGCTATGGAAGAGAATCCATACTATTTACTTGCCAATGCAGCATCGGGAATTGCCGTGAATGATGATTGCAAGCTGAAGTTTTTGGAGCTCAAGGCAAAAAGAACCTATCGTTTTATCGTGTTTAAGATTGAGGAGAAGCAAAAGCAAGTTGTAGTAGAGAAAGTTGGCGAACCAACTCAAAGTTATGAGGACTTTGCTGCTAGCCTCCCTTCTGACGAATGTCGTTACGCCATCTATGATTTCGACTTTGTTACTGAAGAGAACTGTCAAAAGAGTAGAATCTTCTTCATTGCCTGGTCCCCTGATAATTCAAGAGTTAGAAGCAAGATGATATATGCCAGCTCCAAAGACAGATTCAAGAGAGAGCTGGATGGATTCCAGGTTGAGCTGCAAGCTACGGATCCGACTGAGATGGGTCTCGACGTCATTCGAAGCCGAGCTGGCTGA

Protein sequence

MRENPILPVWLYPQSCVAMEENPYYLLANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLPSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRAG

Homology

[Close]

BLAST of Lag0027136 vs. NCBI nr
Match: XP_022945978.1 (actin-depolymerizing factor 1-like isoform X1 [Cucurbita moschata] >XP_022945980.1 actin-depolymerizing factor 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 143/167 (85.63%), Postives = 148/167 (88.62%), Query Frame = 0


Query: 1   MRENPILP--VWLYPQSCVAMEENPYYLLANAASGIAVNDDCKLKFLELKAKRTYRFIVF 60
           M ENPILP    L     +   ++     ANAASG+AVNDDCKLKFL LKAKRTYRFIVF
Sbjct: 1   MGENPILPHIPMLRDHLILTKYDHTMMAKANAASGMAVNDDCKLKFLALKAKRTYRFIVF 60

Query: 61  KIEEKQKQVVVEKVGEPTQSYEDFAASLPSDECRYAIYDFDFVTEENCQKSRIFFIAWSP 120
           KIEEKQKQVVVEKVGEPTQSYEDFAASLPSDECRYA+YDFDFVTEENCQKSRIFFIAWSP
Sbjct: 61  KIEEKQKQVVVEKVGEPTQSYEDFAASLPSDECRYAVYDFDFVTEENCQKSRIFFIAWSP 120

Query: 121 DNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRAG 166
           DNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRAG
Sbjct: 121 DNSRVRSKMIYASSKDRFKRELDGFQVELQATDPTEMGLDVIRSRAG 167

[Close]

BLAST of Lag0027136 vs. NCBI nr
Match: XP_038905295.1 (actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905297.1 actin-depolymerizing factor 2-like [Benincasa hispida])

HSP 1 Score: 273.9 bits (699), Expect = 9.3e-70
Identity = 136/139 (97.84%), Postives = 138/139 (99.28%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGMAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 147 LQATDPTEMGLDVIRSRAG 166
           LQATDPTEMGLDVIRSR G
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

[Close]

BLAST of Lag0027136 vs. NCBI nr
Match: XP_022935027.1 (actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023528602.1 actin-depolymerizing factor 2-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6580558.1 Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.9 bits (699), Expect = 9.3e-70
Identity = 136/139 (97.84%), Postives = 138/139 (99.28%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL
Sbjct: 1   MANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P+DECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE
Sbjct: 61  PADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 120

Query: 147 LQATDPTEMGLDVIRSRAG 166
           LQATDPTEMGLDVIRSR G
Sbjct: 121 LQATDPTEMGLDVIRSRVG 139

[Close]

BLAST of Lag0027136 vs. NCBI nr
Match: KAG7017313.1 (Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.1 bits (697), Expect = 1.6e-69
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 28  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 87
           ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP
Sbjct: 66  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 125

Query: 88  SDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 147
           +DECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL
Sbjct: 126 ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 185

Query: 148 QATDPTEMGLDVIRSRAG 166
           QATDPTEMGLDVIRSR G
Sbjct: 186 QATDPTEMGLDVIRSRVG 203

[Close]

BLAST of Lag0027136 vs. NCBI nr
Match: XP_023528601.1 (actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.1 bits (697), Expect = 1.6e-69
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 28  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 87
           ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP
Sbjct: 47  ANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASLP 106

Query: 88  SDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 147
           +DECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL
Sbjct: 107 ADECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVEL 166

Query: 148 QATDPTEMGLDVIRSRAG 166
           QATDPTEMGLDVIRSR G
Sbjct: 167 QATDPTEMGLDVIRSRVG 184

[Close]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot
Match: Q9FVI1 (Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 3.2e-65
Identity = 120/139 (86.33%), Postives = 133/139 (95.68%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AV+DDCKLKFLELKAKRTYRFI++KIEEKQK+VVVEK+GEPT+SYEDF A L
Sbjct: 1   MANAASGMAVHDDCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P+DECRYA+YDFDF+T+EN QKSRIFFIAWSPD +RVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 147 LQATDPTEMGLDVIRSRAG 166
           LQATDPTEMGLDV RSRAG
Sbjct: 121 LQATDPTEMGLDVFRSRAG 139

[Close]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot
Match: Q9FVI2 (Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 9.4e-65
Identity = 120/138 (86.96%), Postives = 132/138 (95.65%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEK+GEPT+SYEDFAASL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P +ECRYA+YDFDFVT ENCQKSRIFFIAW PD +RVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PENECRYAVYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVE 120

Query: 147 LQATDPTEMGLDVIRSRA 165
           LQA DPTEMGLDVI+SRA
Sbjct: 121 LQACDPTEMGLDVIQSRA 138

[Close]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot
Match: Q0DLA3 (Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 PE=3 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 5.1e-63
Identity = 116/138 (84.06%), Postives = 132/138 (95.65%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AV+D+CKLKFLELKAKRTYRFI++KI+EK+K VVVEKVGEP  +Y+DFAASL
Sbjct: 1   MANAASGMAVDDECKLKFLELKAKRTYRFIIYKIDEKKKMVVVEKVGEPVLNYDDFAASL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P++ECRYAI+D+DFVTEENCQKS+IFFIAWSPD SRVRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PANECRYAIFDYDFVTEENCQKSKIFFIAWSPDTSRVRSKMIYASSKDRFKRELDGIQVE 120

Query: 147 LQATDPTEMGLDVIRSRA 165
           LQATDPTE+GLDVIR RA
Sbjct: 121 LQATDPTEVGLDVIRGRA 138

[Close]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot
Match: Q39250 (Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.0e-62
Identity = 117/138 (84.78%), Postives = 130/138 (94.20%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQVVVEKVG+P Q+YE+FAA L
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P+DECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 147 LQATDPTEMGLDVIRSRA 165
           LQATDPTEM LDV RSRA
Sbjct: 121 LQATDPTEMDLDVFRSRA 138

[Close]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot
Match: Q9ZSK3 (Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 2.5e-62
Identity = 116/137 (84.67%), Postives = 130/137 (94.89%), Query Frame = 0


Query: 27  LANAASGIAVNDDCKLKFLELKAKRTYRFIVFKIEEKQKQVVVEKVGEPTQSYEDFAASL 86
           +ANAASG+AV+DDCKL+FLELKAKRT+RFIV+KIEEKQKQV+VEKVGEP  +YEDFAASL
Sbjct: 1   MANAASGMAVHDDCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASL 60

Query: 87  PSDECRYAIYDFDFVTEENCQKSRIFFIAWSPDNSRVRSKMIYASSKDRFKRELDGFQVE 146
           P+DECRYAIYDFDFVT ENCQKS+IFFIAW PD ++VRSKMIYASSKDRFKRELDG QVE
Sbjct: 61  PADECRYAIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVE 120

Query: 147 LQATDPTEMGLDVIRSR 164
           LQATDPTEM LDV++SR
Sbjct: 121 LQATDPTEMDLDVLKSR 137

The following BLAST results are available for this feature:

BLAST of Lag0027136 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945978.1	1.1e-70	85.63	actin-depolymerizing factor 1-like isoform X1 [Cucurbita moschata] >XP_022945980...	[more]
XP_038905295.1	9.3e-70	97.84	actin-depolymerizing factor 2-like [Benincasa hispida] >XP_038905296.1 actin-dep...	[more]
XP_022935027.1	9.3e-70	97.84	actin-depolymerizing factor 2-like [Cucurbita moschata] >XP_023528602.1 actin-de...	[more]
KAG7017313.1	1.6e-69	98.55	Actin-depolymerizing factor 2, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_023528601.1	1.6e-69	98.55	actin-depolymerizing factor 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0027136 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVI1	3.2e-65	86.33	Actin-depolymerizing factor 2 OS=Petunia hybrida OX=4102 GN=ADF2 PE=2 SV=1	[more]
Q9FVI2	9.4e-65	86.96	Actin-depolymerizing factor 1 OS=Petunia hybrida OX=4102 GN=ADF1 PE=2 SV=1	[more]
Q0DLA3	5.1e-63	84.06	Actin-depolymerizing factor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF7 P...	[more]
Q39250	2.0e-62	84.78	Actin-depolymerizing factor 1 OS=Arabidopsis thaliana OX=3702 GN=ADF1 PE=1 SV=1	[more]
Q9ZSK3	2.5e-62	84.67	Actin-depolymerizing factor 4 OS=Arabidopsis thaliana OX=3702 GN=ADF4 PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002108	Actin-depolymerising factor homology domain	SMART	SM00102	adf_2	coord: 38..165 e-value: 1.7E-59 score: 213.7
IPR002108	Actin-depolymerising factor homology domain	PFAM	PF00241	Cofilin_ADF	coord: 40..162 e-value: 3.8E-37 score: 126.8
IPR002108	Actin-depolymerising factor homology domain	PROSITE	PS51263	ADF_H	coord: 31..165 score: 39.719551
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 27..165 e-value: 1.6E-53 score: 182.0
None	No IPR available	PANTHER	PTHR11913:SF74	ACTIN-DEPOLYMERIZING FACTOR 2-LIKE	coord: 27..164
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 29..163
IPR017904	ADF/Cofilin	PANTHER	PTHR11913	COFILIN-RELATED	coord: 27..164
IPR017904	ADF/Cofilin	CDD	cd11286	ADF_cofilin_like	coord: 32..164 e-value: 4.42004E-68 score: 201.246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027136.1	Lag0027136.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030042	actin filament depolymerization
cellular_component	GO:0015629	actin cytoskeleton
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051015	actin filament binding
molecular_function	GO:0003779	actin binding