Homology
BLAST of Lag0026651 vs. NCBI nr
Match:
XP_022931674.1 (putative disease resistance protein RGA1 [Cucurbita moschata] >XP_022931675.1 putative disease resistance protein RGA1 [Cucurbita moschata])
HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 783/1152 (67.97%), Postives = 930/1152 (80.73%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL VA SL TKLG A+ E QL WGV EL+KLK TI MEAVL DAEEQQ SH
Sbjct: 1 MAESILFHVAASLATKLGSPAITEFQLFWGVHDELDKLKHTILTMEAVLLDAEEQQSNSH 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VKNWIS+++DAFY+V+DLIDEF+YETL+ QVMAK K KEVR+VFS P+QIAFR K+
Sbjct: 61 AVKNWISRVKDAFYEVDDLIDEFTYETLRHQVMAKGKRGVKEVRQVFSLPHQIAFRFKIS 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+I++ REKLKAIDADKNQF+FSER+I++RDDELR+ RE+ SFI DDEV+GR+ DKKAII
Sbjct: 121 HKIRDVREKLKAIDADKNQFYFSERVINTRDDELRKSRETISFICDDEVVGRNPDKKAII 180
Query: 181 DLLLDTNV-VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
DLLL+T++ KEN VIAIVG+GGLGKTALAQSVYNNE ITKHF+LKLWVC+SEDFD+KV
Sbjct: 181 DLLLNTDLDTKENIGVIAIVGMGGLGKTALAQSVYNNENITKHFDLKLWVCVSEDFDIKV 240
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
IVEKIIESATRKKP+ LQMD LQS+LR I GKKYLLIMDDVWNED+EKWVNLKRLLMGG
Sbjct: 241 IVEKIIESATRKKPEILQMDSLQSDLRTHINGKKYLLIMDDVWNEDYEKWVNLKRLLMGG 300
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
GSRILITTRH+RVAE FD S Y L LD++SAWLLF+KMA G EE KELENSNLV+
Sbjct: 301 TSGSRILITTRHRRVAETFDTTSCYDLGELDDKSAWLLFKKMALGSEEPGKELENSNLVD 360
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIVVKL+G+PLAIRTIGRLLY KK E HLWL+FKDKEL RV+EQREET+F+ SILEL
Sbjct: 361 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWLTFKDKELSRVLEQREETQFILSILEL 420
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLPS+LKQCFLYC LFPKDY+I+KDEVIKQW+AQGF+Q+ G I P DIGEDYFMELL
Sbjct: 421 SYHHLPSSLKQCFLYCTLFPKDYNIRKDEVIKQWVAQGFVQSVGVIKPYDIGEDYFMELL 480
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVTRNE G+I++ K+HD M+DLACSIV EYVS+ +N V+ RTRHISI +F
Sbjct: 481 SRSFFQDVTRNEMGDIIKFKIHDLMNDLACSIVKKEYVSMDVNDKVVTGRTRHISIYHNF 540
Query: 541 GIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLS-----LTDVEKL 600
GI+LE L+ L++AKNLRTLII+ TKD QKVL+ IP KFLRLRTLS + + L
Sbjct: 541 GIKLENLKLLYEAKNLRTLIID--APTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVL 600
Query: 601 PESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRY 660
P+SI KLKHLR+L+I +L+FLPNSITKLYNLETL++ C L+ELP+DAKNW+ LRY
Sbjct: 601 PKSIGKLKHLRYLNIC-LYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRY 660
Query: 661 LNLAENFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHM 720
L+L N +I+FLPDSI L NLE LIL DC L ELPKDIK +NLKHLDL GC LTH+
Sbjct: 661 LSLTGNTEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHL 720
Query: 721 PKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQ 780
PKGL EL +LQTM LF + KD G +LSELNRL+KL GSLTI+GL+ CT DL+ + NL+
Sbjct: 721 PKGLGELTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLR 780
Query: 781 LKSGLQKLKLDWSYSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSS 840
LK G Q+LK W+YS+DE ++ LDN+CK VL CLQPHSNV +I ICGY V LC+WLSS
Sbjct: 781 LKLGFQELKFVWNYSKDEHMNIVLDNECKGVLNCLQPHSNVGKIDICGYPEVMLCDWLSS 840
Query: 841 STLVHLVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLET 900
+TL+HLVSIK+S CF+LQ LPQFDQFP LK L L SLPCI+YI Y SSS + PSLE
Sbjct: 841 NTLLHLVSIKLSDCFQLQVLPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEK 900
Query: 901 LSIVNMHNLRGWWKGETSSESSQNN----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDI 960
LSI++MHNL+GWWKGETSSE S NN +T+SCL +L I +CP+LASIPWHAP + L+I
Sbjct: 901 LSIIDMHNLKGWWKGETSSEGSPNNASFPITMSCLCELKIDDCPQLASIPWHAPLKLLEI 960
Query: 961 HSVSSQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQ 1020
+VS QLL+++++ T + S+LS + LS+I+D+EFLP+E FYHFTNL L I +CK+LQ
Sbjct: 961 CAVSLQLLNVIIEKTANPS-SKLSHVYLSKIDDIEFLPRE-FYHFTNLLFLVIEDCKNLQ 1020
Query: 1021 MSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPN 1080
+SS+LVQ +DVLWKEF+SL L L+ IPKL+YLPNG+QHV LQ I I SCPN
Sbjct: 1021 LSSALVQ-----HSTNDVLWKEFQSLHVLFLRAIPKLEYLPNGMQHVTTLQHIGINSCPN 1080
Query: 1081 LITLPQWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGED 1140
+TLP+WI +LTSLS L I NCPKLTSLPE I HL SL+ LTIW+ PKL ER K+GKGE+
Sbjct: 1081 FVTLPKWIHNLTSLSSLSIFNCPKLTSLPEGIHHLRSLESLTIWNSPKLIERYKKGKGEE 1140
Query: 1141 WPKISHVREVEI 1142
W KISH+ + +
Sbjct: 1141 WQKISHIPHINL 1142
BLAST of Lag0026651 vs. NCBI nr
Match:
XP_023539563.1 (uncharacterized protein LOC111800203 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 774/1155 (67.01%), Postives = 917/1155 (79.39%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL VATSL+TKLG LA++E QL GV EL KL TI M+AVL DAEE+QF +
Sbjct: 1 MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VKNWISK+++AFYD EDLIDEF+YETL++QV+A DK AKEVR+ FS P++IAFR +M
Sbjct: 61 EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+IK+ R+ LKAIDADKNQF+FSERMI++RDDELR+RRE+SSFI DDEV+GRD DKKAII
Sbjct: 121 HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEVVGRDHDKKAII 180
Query: 181 DLLLDTNV-VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
DLLL+T+ VKEN AVIAIVG+GGLGKT LAQ VYNNE I KHFELKLWVCIS++FDVKV
Sbjct: 181 DLLLNTDPNVKENVAVIAIVGMGGLGKTTLAQFVYNNEDIKKHFELKLWVCISDEFDVKV 240
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
IVEKI+ESATRKKP+ LQMDLLQS+LR+ I GKKYLLIMDDVWNED+E+ V LKRLLMGG
Sbjct: 241 IVEKILESATRKKPEILQMDLLQSDLRKHIDGKKYLLIMDDVWNEDYERSVALKRLLMGG 300
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
A GSRILITTR Q+VAE FD S Y L LD+ SAWLLF++MAFGGEE EKE +VE
Sbjct: 301 ASGSRILITTRQQKVAETFDTTSSYALGVLDDNSAWLLFKRMAFGGEEPEKE-----VVE 360
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIVVKL+G+PLAIRTIGRLLY KK E HLWL+FKDKEL +V+EQREE +FMRSIL L
Sbjct: 361 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWLAFKDKELSQVLEQREEGEFMRSILGL 420
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLPS+LKQCFLYCALFPKDYDI+KDEVI+QWMAQGF+++ G + +DIGE+YFMELL
Sbjct: 421 SYNHLPSHLKQCFLYCALFPKDYDIEKDEVIRQWMAQGFVRSTGAMEASDIGEEYFMELL 480
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVTRNE G + ECKMHD +HDL CSIV +EYVS+G+N ++ RHISID +F
Sbjct: 481 SRSFFQDVTRNEMGIVTECKMHDLIHDLVCSIVEDEYVSMGVNDSTDIDKIRHISIDNNF 540
Query: 541 GIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSL----TDVEK-L 600
+LE L+ LFKAKNLRTLII+ +D QKV++ I +FL LRTL++ T+ K L
Sbjct: 541 AEKLESLKLLFKAKNLRTLIIDAV--KEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVL 600
Query: 601 PESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRY 660
P SI KLKHLR+L I LEFLPNSITKLYNLE L++ C L+ELP+DAKNW+ LRY
Sbjct: 601 PRSIGKLKHLRYLRIYQR-KLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRY 660
Query: 661 LNLAENFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHM 720
L+LA N +I+FLP S+ L+NLE LIL DC L ELPKDIK +NLKHLDL GC LTHM
Sbjct: 661 LSLARNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHM 720
Query: 721 PKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQ 780
KGL EL SLQTM LF +KKD G +LSELNRLNKL G LTI+GL+ CT DLEK+S N++
Sbjct: 721 SKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMR 780
Query: 781 LKSGLQKLKLDWSYSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSS 840
K G+Q+L L W+YS+DE ++T +DN+C VL CLQPHS V++I IC Y GV LC+WLSS
Sbjct: 781 QKLGVQELNLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSS 840
Query: 841 STLVHLVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLET 900
+TLVHLVSIK+S CFKLQ LPQFDQFPFLKYL L SLP I+Y +Y SSS + PSLE
Sbjct: 841 NTLVHLVSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEK 900
Query: 901 LSIVNMHNLRGWWKGETSSESSQNN----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDI 960
LSIV M NL+GWWKGETS ESS NN + +S L +L I CP LAS PWHA +SL +
Sbjct: 901 LSIVEMLNLKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRL 960
Query: 961 HSVSSQLLDMVMKATTTTTPSELSSLCLS--EIEDLEFLPKELFYHFTNLKHLEIINCKS 1020
H++SSQLL+++ + T E SS +S +I+DLEFLP+ FYHFTNL+ LEI +CK+
Sbjct: 961 HAISSQLLNVIFERTANHCVEECSSTFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKN 1020
Query: 1021 LQMSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSC 1080
L+MSS+LV QHPI +DVLWKEFRSLR L LK IPKL+YLPNG+QHV L+ I I SC
Sbjct: 1021 LRMSSALV-QHPI----NDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVTTLEDIKIISC 1080
Query: 1081 PNLITLPQWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKG 1140
PNL TLP+WI + TSLS L I +CPKLTSL E I HL SLQ L IW CPKLKER ++G+G
Sbjct: 1081 PNLTTLPEWIGNFTSLSELMIISCPKLTSL-ERIYHLPSLQSLYIWVCPKLKERYEQGEG 1140
Query: 1141 EDWPKISHVREVEIK 1143
EDWPKISH+ V I+
Sbjct: 1141 EDWPKISHIPYVSIE 1141
BLAST of Lag0026651 vs. NCBI nr
Match:
XP_022940308.1 (putative disease resistance protein RGA4 [Cucurbita moschata])
HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 783/1258 (62.24%), Postives = 911/1258 (72.42%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL +VA SLVTKLG A+ E QL W +L+KLK TI AMEAVLHDAE+QQ SH
Sbjct: 54 MAESILFNVAASLVTKLGSPAITEFQLFWSFHDDLDKLKQTILAMEAVLHDAEKQQSNSH 113
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VKNWIS++++AFYDV+DLIDE++YETL+ QVMA K KEVRKVFS P QI FR +M
Sbjct: 114 AVKNWISRVKEAFYDVDDLIDEYTYETLRLQVMADGKRWTKEVRKVFSLPRQIEFRFQMS 173
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+IK+ REKLKAID DKNQFH SE MI++RDDELR+RRE+SSFI DD+V+GRD DKKAII
Sbjct: 174 HKIKDIREKLKAIDDDKNQFHLSECMINTRDDELRKRRETSSFICDDDVVGRDGDKKAII 233
Query: 181 DLLLDTNV-VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
D LL+T VKEN AVIAIVG+GGLGKTALAQSVYNNE I KHFEL LWVCIS+DFDVKV
Sbjct: 234 DYLLNTYPNVKENVAVIAIVGMGGLGKTALAQSVYNNEGIRKHFELTLWVCISDDFDVKV 293
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
IVEKI+ESAT+ K + LQMDLLQSELR+ I GKKYLLIMDDVWNED+EKWV LKRLLMGG
Sbjct: 294 IVEKILESATKHKSEILQMDLLQSELRKNIDGKKYLLIMDDVWNEDYEKWVTLKRLLMGG 353
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
A GSRILITTRH+RVAE FD S Y L LD+++AWLLF+KMAFGGEE +KE LV+
Sbjct: 354 ASGSRILITTRHRRVAETFDTTSYYALGELDDKNAWLLFKKMAFGGEEPKKE-----LVD 413
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIVVKL+G+PLAIRTIGRLLY KK E HLW +FK+ EL RV+EQREET+FM SILEL
Sbjct: 414 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWSTFKENELSRVLEQREETQFMLSILEL 473
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLPSNLKQCFLYCALFPKDYDIQKDEVI+QWMA GF+Q+ GTI PNDIGEDYFMELL
Sbjct: 474 SYNHLPSNLKQCFLYCALFPKDYDIQKDEVIRQWMAHGFVQSTGTIKPNDIGEDYFMELL 533
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVTRNE G I+ CKMHD +HDLACSIV +EYVS+ + ++ RTRHI I F
Sbjct: 534 SRSFFQDVTRNEMGIIIYCKMHDLIHDLACSIVEDEYVSMDVKDKLVTERTRHILILKEF 593
Query: 541 GIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSL----TDVEK-L 600
GI+LE L+ LFKAKNL+TLII+ +D QKV++ I +FL LRTL + T+ K L
Sbjct: 594 GIKLESLKLLFKAKNLKTLIIDAV--NEDCQKVVNLISTQFLCLRTLKVEFCYTNTSKVL 653
Query: 601 PESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCS----------------- 660
P SI KLKHLR+L I LEFLPNSITKLYNLE L++ C
Sbjct: 654 PSSIGKLKHLRYLRICQR-KLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLRY 713
Query: 661 ------------------------------------------------------------ 720
Sbjct: 714 LSLDRNVNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKFL 773
Query: 721 --------------------LEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNLEA 780
L+ELP++AKNW+ LRYL+L N +I+FLPD I L+NLE
Sbjct: 774 PDSIGALHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLET 833
Query: 781 LILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGY 840
LIL DC L ELPKDIK +NLKHL+LSGCT+LTHMPKGL EL SLQTM LF +KKD G
Sbjct: 834 LILQDCSELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGC 893
Query: 841 ELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSEDELVSTAL 900
+LSELNRL+KL GSLTI+GL+ CT LEK +L LK G+Q+L+L W+YSED+ ++ L
Sbjct: 894 DLSELNRLDKLEGSLTIEGLEVCTFDVLEK---SLPLKLGVQELELVWNYSEDQDMNIVL 953
Query: 901 DNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQFD 960
DN+CK + LQPHSN+R I ICGY VRLC+WLSS+T VHLVSI++S CFKLQ LPQFD
Sbjct: 954 DNECKGGFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFD 1013
Query: 961 QFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLETLSIVNMHNLRGWWKGETSSESSQN 1020
QFPFLKYL L SLP I+YI +Y SSS + PSLE LSIV M NL+GWWKGETS ESS N
Sbjct: 1014 QFPFLKYLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPN 1073
Query: 1021 N----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTTPSELS 1080
N + +S L +L I CP LAS PWHA +SL +H++SSQLL++V + T E
Sbjct: 1074 NASFPIAMSRLCKLEIDACPHLASFPWHALLKSLSLHAISSQLLNVVFERTANHCVEECF 1133
Query: 1081 SLCLS--EIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQ-------QHPITEDD 1140
S +S +I+DLEFLP+ FYHFTNL+ LEI +CK+LQMSS+LVQ QHPI
Sbjct: 1134 STFVSFDKIDDLEFLPEGFFYHFTNLEDLEIKDCKNLQMSSALVQHPINSLVQHPI---- 1193
Query: 1141 DDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITLPQWISDLTSLS 1143
+DVLWKEFRSLR L L+ IPKL+YLPNG+QHV L+ I I SCPNLITLP+WI D TSL
Sbjct: 1194 NDVLWKEFRSLRLLYLEAIPKLEYLPNGMQHVTTLERISIISCPNLITLPEWIGDFTSLL 1253
BLAST of Lag0026651 vs. NCBI nr
Match:
XP_023547023.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 649/920 (70.54%), Postives = 762/920 (82.83%), Query Frame = 0
Query: 17 LGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDV 76
L + + QLLWG+D EL++LK T+ AMEAVL DAEEQQ KSH VKNWIS++++AFYDV
Sbjct: 28 LSWCGNSKFQLLWGMDDELDQLKHTLSAMEAVLLDAEEQQSKSHAVKNWISRVKEAFYDV 87
Query: 77 EDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMIRRIKETREKLKAIDAD 136
+DLIDEF+YETL++QV+ K K AKEVR+VFS P+Q+AFR +M +IK+ +EKLKAID D
Sbjct: 88 DDLIDEFAYETLRRQVITKGKRGAKEVREVFSIPHQVAFRFEMSHKIKDIKEKLKAIDVD 147
Query: 137 KNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNV-VKENTAV 196
K+QFHFSERMI+ RDDELR+ RE+SSFI DDEV+GRDDD+KAIIDLLL+T+ KEN AV
Sbjct: 148 KSQFHFSERMINIRDDELRKSRETSSFICDDEVVGRDDDRKAIIDLLLNTDTNDKENVAV 207
Query: 197 IAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQT 256
IAIVG+GGLGKTALAQSVYNNE ITKHFELKLWVCISEDFDVKVIVEKIIESAT KKP+
Sbjct: 208 IAIVGMGGLGKTALAQSVYNNEYITKHFELKLWVCISEDFDVKVIVEKIIESATTKKPEI 267
Query: 257 LQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVA 316
LQMDLLQSELR I GKKYLLIMDDVWNEDHEKWV LKRLLMGGA GSRILITTR QRVA
Sbjct: 268 LQMDLLQSELRRHIDGKKYLLIMDDVWNEDHEKWVTLKRLLMGGAPGSRILITTRPQRVA 327
Query: 317 ENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAI 376
E FD S Y L LD++SAWLLF+KMAFGGEESEKELENS L+EIGKEI+VKL+G+PLAI
Sbjct: 328 ETFDTTSSYALGVLDDKSAWLLFKKMAFGGEESEKELENSKLIEIGKEILVKLKGLPLAI 387
Query: 377 RTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYC 436
RTIGRLL+ KK E HLWL+FK+ EL RV+EQREE +FM SILELS+ HL S+LKQCFLYC
Sbjct: 388 RTIGRLLHAKKPEHHLWLAFKENELSRVLEQREEAQFMLSILELSFNHLRSSLKQCFLYC 447
Query: 437 ALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNI 496
ALFPKDY+IQKDEVIKQW+AQGF+Q+ G I P DIGEDYFMELLSRSFFQDVTRNE G +
Sbjct: 448 ALFPKDYNIQKDEVIKQWVAQGFVQSIGIIEPYDIGEDYFMELLSRSFFQDVTRNEMGIV 507
Query: 497 VECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFGIELELLESLFKAKNL 556
ECKMHD +HDLACSIV NEYVS+G+N + +RTRHISI F ++LE ++ LF+AKNL
Sbjct: 508 TECKMHDLIHDLACSIVKNEYVSMGVNDSTVIDRTRHISIYHRFSMKLESIKLLFEAKNL 567
Query: 557 RTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSLT-----DVEKLPESICKLKHLRFLSIS 616
RTLII+ KD +KVL+ IP KFLRLRTLSL + LP +I KLKHLR+L I
Sbjct: 568 RTLIID--ARDKDCRKVLNLIPLKFLRLRTLSLNFSYAITSKVLPRAIGKLKHLRYLKIR 627
Query: 617 DSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRYLNLAENFDIEFLPDSI 676
+L+FLPNSITKLYNLETL++ DC L+ELP+DAKNW+ LRYL+LA N +I++LPDSI
Sbjct: 628 -LFELQFLPNSITKLYNLETLVLDDCKLLQELPRDAKNWMKLRYLSLARNKEIKYLPDSI 687
Query: 677 TELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLF 736
L+NLE LIL DC L ELPKDIK +NLKHLDL GC LTHMPKGL EL LQTM LF
Sbjct: 688 AALHNLETLILQDCSLLKELPKDIKKCVNLKHLDLRGCHALTHMPKGLGELTRLQTMNLF 747
Query: 737 SIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSE 796
+KKD G +LSELNRL+KL GSLTI+GL+ CT DLEK+S NL+L SG+Q+LKL W+ S+
Sbjct: 748 VLKKDIGCDLSELNRLDKLKGSLTIKGLEVCTTDDLEKSSFNLRLMSGVQELKLVWNNSK 807
Query: 797 DELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFK 856
DE ++ LDN+ K VL+CLQPHS VR I ICGY VRLC+WLSS+TL+HLVSIK+S CFK
Sbjct: 808 DEHMNIVLDNEGKGVLQCLQPHSKVRNIDICGYREVRLCDWLSSNTLIHLVSIKLSDCFK 867
Query: 857 LQHLPQFDQFPFLKYLCLTSLPCIDYI-VKKDYLSSSKYLPSLETLSIVNMHNLRGWWKG 916
LQ LPQFDQFPFLK+L L SLP I+Y+ +Y SSS +LPSLE LS+++MHNL+GWWKG
Sbjct: 868 LQALPQFDQFPFLKHLSLKSLPNIEYMDNNNEYSSSSMFLPSLEKLSLIDMHNLKGWWKG 927
Query: 917 ETSSESSQNN----LTVSCL 925
ETSSE+S +N +T+SC+
Sbjct: 928 ETSSEASPDNASFPITMSCI 944
BLAST of Lag0026651 vs. NCBI nr
Match:
XP_023547024.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023547025.1 disease resistance protein RGA2-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1232.6 bits (3188), Expect = 0.0e+00
Identity = 643/906 (70.97%), Postives = 754/906 (83.22%), Query Frame = 0
Query: 31 VDAELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEFSYETLKQ 90
+D EL++LK T+ AMEAVL DAEEQQ KSH VKNWIS++++AFYDV+DLIDEF+YETL++
Sbjct: 1 MDDELDQLKHTLSAMEAVLLDAEEQQSKSHAVKNWISRVKEAFYDVDDLIDEFAYETLRR 60
Query: 91 QVMAKDKTLAKEVRKVFSKPNQIAFRLKMIRRIKETREKLKAIDADKNQFHFSERMISSR 150
QV+ K K AKEVR+VFS P+Q+AFR +M +IK+ +EKLKAID DK+QFHFSERMI+ R
Sbjct: 61 QVITKGKRGAKEVREVFSIPHQVAFRFEMSHKIKDIKEKLKAIDVDKSQFHFSERMINIR 120
Query: 151 DDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNV-VKENTAVIAIVGIGGLGKTAL 210
DDELR+ RE+SSFI DDEV+GRDDD+KAIIDLLL+T+ KEN AVIAIVG+GGLGKTAL
Sbjct: 121 DDELRKSRETSSFICDDEVVGRDDDRKAIIDLLLNTDTNDKENVAVIAIVGMGGLGKTAL 180
Query: 211 AQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDLLQSELREQI 270
AQSVYNNE ITKHFELKLWVCISEDFDVKVIVEKIIESAT KKP+ LQMDLLQSELR I
Sbjct: 181 AQSVYNNEYITKHFELKLWVCISEDFDVKVIVEKIIESATTKKPEILQMDLLQSELRRHI 240
Query: 271 VGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENFDPISLYHLANL 330
GKKYLLIMDDVWNEDHEKWV LKRLLMGGA GSRILITTR QRVAE FD S Y L L
Sbjct: 241 DGKKYLLIMDDVWNEDHEKWVTLKRLLMGGAPGSRILITTRPQRVAETFDTTSSYALGVL 300
Query: 331 DNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRLLYDKKSEF 390
D++SAWLLF+KMAFGGEESEKELENS L+EIGKEI+VKL+G+PLAIRTIGRLL+ KK E
Sbjct: 301 DDKSAWLLFKKMAFGGEESEKELENSKLIEIGKEILVKLKGLPLAIRTIGRLLHAKKPEH 360
Query: 391 HLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKDYDIQKDEV 450
HLWL+FK+ EL RV+EQREE +FM SILELS+ HL S+LKQCFLYCALFPKDY+IQKDEV
Sbjct: 361 HLWLAFKENELSRVLEQREEAQFMLSILELSFNHLRSSLKQCFLYCALFPKDYNIQKDEV 420
Query: 451 IKQWMAQGFIQTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMHDFMHDLAC 510
IKQW+AQGF+Q+ G I P DIGEDYFMELLSRSFFQDVTRNE G + ECKMHD +HDLAC
Sbjct: 421 IKQWVAQGFVQSIGIIEPYDIGEDYFMELLSRSFFQDVTRNEMGIVTECKMHDLIHDLAC 480
Query: 511 SIVGNEYVSIGMNHMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIEEALSTKDI 570
SIV NEYVS+G+N + +RTRHISI F ++LE ++ LF+AKNLRTLII+ KD
Sbjct: 481 SIVKNEYVSMGVNDSTVIDRTRHISIYHRFSMKLESIKLLFEAKNLRTLIID--ARDKDC 540
Query: 571 QKVLDNIPNKFLRLRTLSLT-----DVEKLPESICKLKHLRFLSISDSIDLEFLPNSITK 630
+KVL+ IP KFLRLRTLSL + LP +I KLKHLR+L I +L+FLPNSITK
Sbjct: 541 RKVLNLIPLKFLRLRTLSLNFSYAITSKVLPRAIGKLKHLRYLKIR-LFELQFLPNSITK 600
Query: 631 LYNLETLMVIDCS-LEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNLEALILHDC 690
LYNLETL++ DC L+ELP+DAKNW+ LRYL+LA N +I++LPDSI L+NLE LIL DC
Sbjct: 601 LYNLETLVLDDCKLLQELPRDAKNWMKLRYLSLARNKEIKYLPDSIAALHNLETLILQDC 660
Query: 691 ISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGYELSELN 750
L ELPKDIK +NLKHLDL GC LTHMPKGL EL LQTM LF +KKD G +LSELN
Sbjct: 661 SLLKELPKDIKKCVNLKHLDLRGCHALTHMPKGLGELTRLQTMNLFVLKKDIGCDLSELN 720
Query: 751 RLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSEDELVSTALDNDCKR 810
RL+KL GSLTI+GL+ CT DLEK+S NL+L SG+Q+LKL W+ S+DE ++ LDN+ K
Sbjct: 721 RLDKLKGSLTIKGLEVCTTDDLEKSSFNLRLMSGVQELKLVWNNSKDEHMNIVLDNEGKG 780
Query: 811 VLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQFDQFPFLK 870
VL+CLQPHS VR I ICGY VRLC+WLSS+TL+HLVSIK+S CFKLQ LPQFDQFPFLK
Sbjct: 781 VLQCLQPHSKVRNIDICGYREVRLCDWLSSNTLIHLVSIKLSDCFKLQALPQFDQFPFLK 840
Query: 871 YLCLTSLPCIDYI-VKKDYLSSSKYLPSLETLSIVNMHNLRGWWKGETSSESSQNN---- 925
+L L SLP I+Y+ +Y SSS +LPSLE LS+++MHNL+GWWKGETSSE+S +N
Sbjct: 841 HLSLKSLPNIEYMDNNNEYSSSSMFLPSLEKLSLIDMHNLKGWWKGETSSEASPDNASFP 900
BLAST of Lag0026651 vs. ExPASy Swiss-Prot
Match:
Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)
HSP 1 Score: 526.2 bits (1354), Expect = 9.4e-148
Identity = 372/1153 (32.26%), Postives = 580/1153 (50.30%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAE+ L L+ L + E+ L++G + E +KL ++AVL DA+E+Q K
Sbjct: 1 MAEAFL----QVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYET--LKQQVMAKDKTLAKEVRKVFSKPNQIAFRLK 120
+KNW+ KL A Y+V+D++D+ E KQ V+ + P I F K
Sbjct: 61 AIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGR------------YHPRTITFCYK 120
Query: 121 MIRRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKA 180
+ +R+KE EKL AI ++ FH ER+I + RR++ + + +V GR+ ++
Sbjct: 121 VGKRMKEMMEKLDAIAEERRNFHLDERII----ERQAARRQTGFVLTEPKVYGREKEEDE 180
Query: 181 IIDLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVK 240
I+ +L++ E V+ I+G+GGLGKT LAQ V+N+++IT+HF LK+WVC+S+DFD K
Sbjct: 181 IVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEK 240
Query: 241 VIVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMG 300
+++ I+ES K + + LQ +L+E + GK+Y L++DDVWNED EKW NL+ +L
Sbjct: 241 RLIKAIVESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKI 300
Query: 301 GAKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLV 360
GA G+ ILITTR +++ + LY L+NL E WLLF++ AF + + L+
Sbjct: 301 GASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAF----CHQTETSPKLM 360
Query: 361 EIGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILE 420
EIGKEIV K G+PLA +T+G LL K+ E W +D E++ + ++ + L
Sbjct: 361 EIGKEIVKKCGGVPLAAKTLGGLLRFKREESE-WEHVRDSEIWNL---PQDENSVLPALR 420
Query: 421 LSYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMEL 480
LSY HLP +L+QCF YCA+FPKD I+K+ +I WMA F+ + G + D+G + + EL
Sbjct: 421 LSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNEL 480
Query: 481 LSRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLS 540
RSFFQ++ KMHD +HDLA
Sbjct: 481 YLRSFFQEI--EVKSGKTYFKMHDLIHDLA------------------------------ 540
Query: 541 FGIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSLTDVEKLPESI 600
S+F A + S++ I R +++ D E + +
Sbjct: 541 --------TSMFSA----------SASSRSI--------------RQINVKDDEDMMFIV 600
Query: 601 CKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCSLEELPKDAKNWINLRYLNLAE 660
K + + S+ + + P+ + +L L + + E+LP + ++LRYL+L+
Sbjct: 601 TNYKDMMSIGFSEVVS-SYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSG 660
Query: 661 NFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLS 720
N I LP + +L NL+ L L++C SL LPK +L++L L C LT MP +
Sbjct: 661 N-KICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC-PLTSMPPRIG 720
Query: 721 ELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGL 780
L L+T+ F + + GY+L EL LN L G+++I L+ +D+E NL K+ L
Sbjct: 721 LLTCLKTLGYFVVGERKGYQLGELRNLN-LRGAISITHLER-VKNDMEAKEANLSAKANL 780
Query: 781 QKLKLDWS----YSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSS 840
L + W Y +E+ +VLE L+PH N++ + I + G L +W++ S
Sbjct: 781 HSLSMSWDRPNRYESEEV----------KVLEALKPHPNLKYLEIIDFCGFCLPDWMNHS 840
Query: 841 TLVHLVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYL-----P 900
L ++VSI IS C LP F + P CL SL D V+ +Y+ S +L P
Sbjct: 841 VLKNVVSILISGCENCSCLPPFGELP-----CLESLELQDGSVEVEYVEDSGFLTRRRFP 900
Query: 901 SLETLSIVNMHNLRGWWKGETSSESSQNNLTVSCLSQLVIRNCPRLASIPWHAPSQSLDI 960
SL L I NL+G + + + + L ++ I +CP P + + L+I
Sbjct: 901 SLRKLHIGGFCNLKGLQRMKGAEQ-------FPVLEEMKISDCPMFV-FPTLSSVKKLEI 960
Query: 961 HSVSSQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQ 1020
E + LS I +L L T+LK
Sbjct: 961 W-------------------GEADAGGLSSISNLSTL--------TSLK----------- 990
Query: 1021 MSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPN 1080
+ H +T ++ ++K +L YL + + LK LP L + L+ + I C
Sbjct: 1021 ----IFSNHTVTSLLEE-MFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYA 990
Query: 1081 LITLP-QWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGE 1140
L +LP + + L+SL+ LF+E+C L LPE ++HL +L L I CP+L +RC++G GE
Sbjct: 1081 LESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGE 990
Query: 1141 DWPKISHVREVEI 1142
DW KISH+ V I
Sbjct: 1141 DWHKISHIPNVNI 990
BLAST of Lag0026651 vs. ExPASy Swiss-Prot
Match:
Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)
HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-146
Identity = 365/1146 (31.85%), Postives = 585/1146 (51.05%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAE+ L + +L + +G ++ L++G + E EKL ++AVL DA+E+Q K
Sbjct: 1 MAEAFLQVLLENLTSFIG----DKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
++NW+ KL A Y+V+D++ E E ++ ++ R F P I FR K+
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIR----------FEQSRLGFYHPGIINFRHKIG 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
RR+KE EKL AI ++ +FHF E++ + RE+ + + +V GRD ++ I+
Sbjct: 121 RRMKEIMEKLDAISEERRKFHFLEKI--TERQAAAATRETGFVLTEPKVYGRDKEEDEIV 180
Query: 181 DLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVI 240
+L++ V E V I+G+GGLGKT LAQ ++N+E++TKHF K+WVC+S+DFD K +
Sbjct: 181 KILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRL 240
Query: 241 VEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGA 300
++ II + R P + Q +L+E + GK+YLL++DDVWN+D EKW L+ +L GA
Sbjct: 241 IKTIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGA 300
Query: 301 KGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVEI 360
+G+ IL TTR ++V + YHL+NL + LLF + AFG +++ N NLV I
Sbjct: 301 RGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFG----QQKEANPNLVAI 360
Query: 361 GKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILELS 420
GKEIV K G+PLA +T+G LL K+ E W +D E++ + ++E+ + + L LS
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGLLRFKREESE-WEHVRDNEIWSL--PQDESSILPA-LRLS 420
Query: 421 YKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLS 480
Y HLP +L+QCF YCA+FPKD + K+ +I WMA GF+ + G + D+G + + EL
Sbjct: 421 YHHLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYL 480
Query: 481 RSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFG 540
RSFFQ++ ++GN K+HD +HDLA S+ S S G
Sbjct: 481 RSFFQEI-EAKSGN-TYFKIHDLIHDLATSL---------------------FSASASCG 540
Query: 541 IELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSLTDVEKLPESICK 600
N+R + +++ T I
Sbjct: 541 -------------NIREINVKDYKHTVSI------------------------------- 600
Query: 601 LKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCSLEELPKDAKNWINLRYLNLAENF 660
F ++ S + P+ + K +L L + LE+LP + ++LRYL+L+ N
Sbjct: 601 ----GFAAVVSS----YSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN- 660
Query: 661 DIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSEL 720
+ LP+ + +L NL+ L +H+C SL LPK +L+HL + GC LT P + L
Sbjct: 661 NFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGC-PLTSTPPRIGLL 720
Query: 721 PSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQK 780
L+T+ F + GY+L EL LN L GS++I L+ +D + + NL K+ LQ
Sbjct: 721 TCLKTLGFFIVGSKKGYQLGELKNLN-LCGSISITHLER-VKNDTDAEA-NLSAKANLQS 780
Query: 781 LKLDWSYSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLV 840
L + W D ++ +VLE L+PH N++ + I + G R +W++ S L ++
Sbjct: 781 LSMSW----DNDGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVI 840
Query: 841 SIKISRCFKLQHLPQFDQFPFLKYLCLTSLPC-IDYIVKKDY---LSSSKYLPSLETLSI 900
S++I C LP F + P L+ L L + ++Y+ + D S+ + PSL+ L I
Sbjct: 841 SVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRI 900
Query: 901 VNMHNLRGWWKGETSSESSQNNLTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVSSQL 960
+L+G K E + L ++ I CP L P + + L++H ++
Sbjct: 901 WFFRSLKGLMKEEGEEK-------FPMLEEMAILYCP-LFVFPTLSSVKKLEVHGNTN-- 960
Query: 961 LDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQ 1020
++ + S L+SL + LP+E+F TNL+ L + K+
Sbjct: 961 ---TRGLSSISNLSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKN--------- 987
Query: 1021 QHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITLP-Q 1080
LK LP L + AL+ + I SC +L + P Q
Sbjct: 1021 -----------------------------LKDLPTSLTSLNALKRLQIESCDSLESFPEQ 987
Query: 1081 WISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGEDWPKISH 1140
+ LTSL++LF++ C L LPE ++HL +L L + CP++++RC + GEDW KI+H
Sbjct: 1081 GLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAH 987
Query: 1141 VREVEI 1142
+ ++I
Sbjct: 1141 IPNLDI 987
BLAST of Lag0026651 vs. ExPASy Swiss-Prot
Match:
Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)
HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-143
Identity = 361/1129 (31.98%), Postives = 560/1129 (49.60%), Query Frame = 0
Query: 24 EVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEF 83
E+ LL+G E ++L ++AVL DA+E+Q + ++NW+ KL A Y+V+D++DE+
Sbjct: 20 ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEY 79
Query: 84 SYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMIRRIKETREKLKAIDADKNQFHFS 143
+ T + P I FR K+ +R+ + +KLKAI ++ FH
Sbjct: 80 KTKA----------TRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLH 139
Query: 144 ERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNVVKENTAVIAIVGIGG 203
E+++ + RRE+ S + + +V GRD +K I+ +L++ ++ +V+ I+G+GG
Sbjct: 140 EKIV----ERQAVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMGG 199
Query: 204 LGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDL--L 263
LGKT LAQ V+N++++T+HF K+W+C+SEDFD K +++ I+ES +P +MDL L
Sbjct: 200 LGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVES-IEGRPLLGEMDLAPL 259
Query: 264 QSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENFDPI 323
Q +L+E + GK+YLL++DDVWNED +KW NL+ +L GA G+ +L TTR ++V +
Sbjct: 260 QKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSIMGTL 319
Query: 324 SLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRL 383
Y L+NL E WLLF + AFG +E N NLV IGKEIV K G+PLA +T+G +
Sbjct: 320 QPYELSNLSQEDCWLLFMQRAFGHQEE----INPNLVAIGKEIVKKSGGVPLAAKTLGGI 379
Query: 384 LYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKD 443
L K+ E W +D ++ + ++E+ + + L LSY LP +LKQCF YCA+FPKD
Sbjct: 380 LCFKREE-RAWEHVRDSPIWNL--PQDESSILPA-LRLSYHQLPLDLKQCFAYCAVFPKD 439
Query: 444 YDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMH 503
++K+++I WMA GF+ + G + D+G++ + EL RSFFQ++ + KMH
Sbjct: 440 AKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYF--KMH 499
Query: 504 DFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIE 563
D +HDLA S+ S + + ++ T +SI + + L L
Sbjct: 500 DLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFAEVVFFYTLPPL------------ 559
Query: 564 EALSTKDIQKVLDNIPNKFLRLRTLSLTD--VEKLPESICKLKHLRFLSISDSIDLEFLP 623
KF+ LR L+L D KLP SI L HLR+L++ S + LP
Sbjct: 560 ----------------EKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLP 619
Query: 624 NSITKLYNLETLMVIDCSLEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNLEALI 683
+ KL NL+TL D+++
Sbjct: 620 KQLCKLQNLQTL-----------------------------DLQY--------------- 679
Query: 684 LHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGYEL 743
C L LPK+ +L++L L G LT MP + L L+T+ F + + GY+L
Sbjct: 680 ---CTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQL 739
Query: 744 SELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSEDELVSTALDN 803
EL LN L GS+ I L+ +D + NL K L L + W+ + ++
Sbjct: 740 GELGNLN-LYGSIKISHLER-VKNDKDAKEANLSAKGNLHSLSMSWNNFGPHI----YES 799
Query: 804 DCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQFDQF 863
+ +VLE L+PHSN+ + I G+ G+ L W++ S L ++VSI IS LP F
Sbjct: 800 EEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDL 859
Query: 864 PFLKYLCL-TSLPCIDYIVKKDYLSSSKY-----LPSLETLSIVNMHNLRGWWKGETSSE 923
P L+ L L ++Y+ + D S + PSL L I + +L+G K E +
Sbjct: 860 PCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEEQ 919
Query: 924 SSQNNLTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTTPSELS 983
L +++I CP L T ++ L+
Sbjct: 920 -------FPVLEEMIIHECPFL----------------------------TLSSNLRALT 968
Query: 984 SLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQQHPITEDDDDVLWKEFR 1043
SL + + P+E+F + NLK+L I C +
Sbjct: 980 SLRICYNKVATSFPEEMFKNLANLKYLTISRCNN-------------------------- 968
Query: 1044 SLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITLP-QWISDLTSLSRLFIENCP 1103
LK LP L + AL+ + I C L +LP + + L+SL+ LF+E+C
Sbjct: 1040 ------------LKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCN 968
Query: 1104 KLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGEDWPKISHVREVEI 1142
L LPE ++HL +L L I CP+L +RC++G GEDW KISH+ V I
Sbjct: 1100 MLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNI 968
BLAST of Lag0026651 vs. ExPASy Swiss-Prot
Match:
Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)
HSP 1 Score: 511.1 bits (1315), Expect = 3.1e-143
Identity = 361/1138 (31.72%), Postives = 571/1138 (50.18%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAE+ + V +L + L E+ LL+G E ++L ++AVL DA+E+Q
Sbjct: 1 MAEAFIQVVLDNLTSFL----KGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
++NW+ KL A Y+V+D++DE+ + + L E + P I FR K+
Sbjct: 61 PLENWLQKLNAATYEVDDILDEYKTKA--------TRFLQSEYGRY--HPKVIPFRHKVG 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+R+ + +KL AI ++ +FH E++I + RE+ S + + +V GRD +K I+
Sbjct: 121 KRMDQVMKKLNAIAEERKKFHLQEKII----ERQAATRETGSVLTEPQVYGRDKEKDEIV 180
Query: 181 DLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVI 240
+L++T + +V+ I+G+GGLGKT L+Q V+N++++T+ F K+W+CIS+DF+ K +
Sbjct: 181 KILINTASDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRL 240
Query: 241 VEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGA 300
++ I+ES K + + LQ +L+E + GK+Y L++DDVWNED KW NL+ +L GA
Sbjct: 241 IKAIVESIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGA 300
Query: 301 KGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVEI 360
G+ +L TTR ++V + Y L+NL E W LF + AFG +E N NL+ I
Sbjct: 301 SGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEE----INPNLMAI 360
Query: 361 GKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILELS 420
GKEIV K G+PLA +T+G +L K+ E W +D ++ + ++E+ + + L LS
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGILRFKREE-REWEHVRDSPIWNL--PQDESSILPA-LRLS 420
Query: 421 YKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLS 480
Y HLP +L+QCF+YCA+FPKD + K+ +I WMA GF+ + G + D+G + + EL
Sbjct: 421 YHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYL 480
Query: 481 RSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFG 540
RSFFQ++ E+G KMHD +HDLA
Sbjct: 481 RSFFQEI-EVESGK-TYFKMHDLIHDLA-------------------------------- 540
Query: 541 IELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSLTDVEKLPESICK 600
SLF A S+ +I+++ N +
Sbjct: 541 ------TSLFSANT----------SSSNIREINANYDGYMM------------------- 600
Query: 601 LKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCSLEELPKDAKNWINLRYLNLAENF 660
+ F + S + P+ + K +L L + + +L +LP + ++LRYL+L+ NF
Sbjct: 601 --SIGFAEVVSS----YSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNF 660
Query: 661 DIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSEL 720
I LP + +L NL+ L LH C SL LPK +L++L L GC+ LT P + L
Sbjct: 661 RIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCS-LTSTPPRIGLL 720
Query: 721 PSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQK 780
L++++ F I K G++L EL LN L GS++I L D + NL K+ L
Sbjct: 721 TCLKSLSCFVIGKRKGHQLGELKNLN-LYGSISITKLDR-VKKDTDAKEANLSAKANLHS 780
Query: 781 LKLDWSYSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLV 840
L L W + VLE L+PHSN++ + I G+ G+RL +W++ S L ++V
Sbjct: 781 LCLSWDLDGKHRYDS-------EVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVV 840
Query: 841 SIKISRCFKLQHLPQFDQFPFLKYLCL-TSLPCIDYIVKKDYLSSSKYLPSLETLSIVNM 900
SI+I C LP F + P L+ L L T ++Y+ +D + ++ PSL L I +
Sbjct: 841 SIRIRGCENCSCLPPFGELPCLESLELHTGSADVEYV--EDNVHPGRF-PSLRKLVIWDF 900
Query: 901 HNLRGWWKGETSSESSQNNLTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVSSQLLDM 960
NL+G K E + L ++ CP IP + ++L + + +L
Sbjct: 901 SNLKGLLKMEGEKQ-------FPVLEEMTFYWCPMFV-IPTLSSVKTLKVIVTDATVLRS 960
Query: 961 VMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQQHP 1020
+ L+SL +S+ + LP+E+F NLK
Sbjct: 961 ISNLRA------LTSLDISDNVEATSLPEEMFKSLANLK--------------------- 972
Query: 1021 ITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITLP-QWIS 1080
YL++ LK LP L + AL+ + C L +LP + +
Sbjct: 1021 -----------------YLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVK 972
Query: 1081 DLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGEDWPKISHV 1137
LTSL+ L + NC L LPE ++HL +L LTI CP + +RC+ G GEDW KI+H+
Sbjct: 1081 GLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCPIVFKRCERGIGEDWHKIAHI 972
BLAST of Lag0026651 vs. ExPASy Swiss-Prot
Match:
Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 398.3 bits (1022), Expect = 3.0e-109
Identity = 329/1055 (31.18%), Postives = 522/1055 (49.48%), Query Frame = 0
Query: 35 LEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMA 94
LE+L + + AVL DAEE+Q + V+ W+++L+D Y ED +D+ + E L+ + A
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KDKTLAKEVRKVFSKPNQIAF----RLKMIRRIKETREKLKAIDADKNQFHFSE--RMIS 154
+ + + +R++ + + F + R+++ +L+ + + +N E MI
Sbjct: 99 ESSS-SNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIP 158
Query: 155 SRDDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNVVKENTAVIAIVGIGGLGKTA 214
++R ++S + + EV GRDDDK I+ L+ N V+AIVGIGG+GKT
Sbjct: 159 ------KQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTT 218
Query: 215 LAQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDLLQSELREQ 274
L+Q +YN++ + +F K+W +SE+FDV I +K+ ES T + + +D+LQ +L+E+
Sbjct: 219 LSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKER 278
Query: 275 IVGK--KYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENFDPISLYHL 334
+ G +LL++DD+WNE+ W L++ + A+GS+IL+TTR QRVA + +++L
Sbjct: 279 LTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNL 338
Query: 335 ANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRLL--YD 394
L + W LF K FG +E N + ++ + IV K RG+PLA++T+G +L
Sbjct: 339 QPLSDGDCWSLFMKTVFGNQE---PCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEG 398
Query: 395 KKSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKDYDI 454
K E+ LS R+ + + + +L +SY +LP++LK+CF YC++FPK +
Sbjct: 399 KVIEWERVLS------SRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAF 458
Query: 455 QKDEVIKQWMAQGFI-QTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMHDF 514
+KD+V+ WMA+GF+ QT + ++G +YF EL SRS Q + +T I MHDF
Sbjct: 459 EKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQ---KTKTRYI----MHDF 518
Query: 515 MHDLACSIVGNEYVSIGMN--HMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIE 574
+++LA E+ S + + ++ RTR++S E E+L + K LRT +
Sbjct: 519 INELA-QFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFL-- 578
Query: 575 EALSTKDIQKVLDNIPNKFLRLRTLSLTDVEKLPESICKLKHLRFLSISDSIDLEFLPNS 634
LSLT+ + S C + + + LP
Sbjct: 579 -----------------------PLSLTNSSR---SCC---------LDQMVSEKLLPT- 638
Query: 635 ITKLYNLETLMVIDCSLEELPKD-AKNWINLRYLNLAENFDIEFLPDSITELYNLEALIL 694
L L L + + LP D KN + R+L+L+ ++E LP S+ +YNL+ L+L
Sbjct: 639 ---LTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLL 698
Query: 695 HDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGYELS 754
C SL ELP DI N INL++LDL G T L MP+ L SLQT+T F + G +S
Sbjct: 699 SYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRIS 758
Query: 755 ELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDW----SYSEDELVSTA 814
EL L+ L G L I L+ + NL K L+++ W S SE+
Sbjct: 759 ELGGLHDLHGKLKIVELQR-VVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHR 818
Query: 815 LDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQF 874
N+ + V E L+PH ++ ++ I Y G R +WLS + +V I++ C LP
Sbjct: 819 TQNEAE-VFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSL 878
Query: 875 DQFPFLKYLCLTSLPCIDYIVKKDYLSSSKY-------LPSLETLSIVNMHNLRGWWKGE 934
Q P LK L ++ + + I +K Y S + SLETL N+ + + W
Sbjct: 879 GQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEW---- 938
Query: 935 TSSESSQNNLTVSCLSQLVIRNCPRL-ASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTT 994
++ +L S L +L I CP L ++P PS + +H LLD
Sbjct: 939 LDVRVTRGDLFPS-LKKLFILRCPELTGTLPTFLPS-LISLHIYKCGLLDF---QPDHHE 998
Query: 995 PSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQQHPITEDDDDVL 1054
S + LS + L K HF NL LE+ C SL S L +H
Sbjct: 999 YSYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSL-YSLELSNEH---------- 1004
Query: 1055 WKEFRSLRYLRLKE------IPKLKYLPNGLQHVI 1058
+ +LR LR+ + +PKL LP LQ I
Sbjct: 1059 LRGPNALRNLRINDCQNLQLLPKLNALPQNLQVTI 1004
BLAST of Lag0026651 vs. ExPASy TrEMBL
Match:
A0A6J1EUW9 (putative disease resistance protein RGA1 OS=Cucurbita moschata OX=3662 GN=LOC111437812 PE=4 SV=1)
HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 783/1152 (67.97%), Postives = 930/1152 (80.73%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL VA SL TKLG A+ E QL WGV EL+KLK TI MEAVL DAEEQQ SH
Sbjct: 1 MAESILFHVAASLATKLGSPAITEFQLFWGVHDELDKLKHTILTMEAVLLDAEEQQSNSH 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VKNWIS+++DAFY+V+DLIDEF+YETL+ QVMAK K KEVR+VFS P+QIAFR K+
Sbjct: 61 AVKNWISRVKDAFYEVDDLIDEFTYETLRHQVMAKGKRGVKEVRQVFSLPHQIAFRFKIS 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+I++ REKLKAIDADKNQF+FSER+I++RDDELR+ RE+ SFI DDEV+GR+ DKKAII
Sbjct: 121 HKIRDVREKLKAIDADKNQFYFSERVINTRDDELRKSRETISFICDDEVVGRNPDKKAII 180
Query: 181 DLLLDTNV-VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
DLLL+T++ KEN VIAIVG+GGLGKTALAQSVYNNE ITKHF+LKLWVC+SEDFD+KV
Sbjct: 181 DLLLNTDLDTKENIGVIAIVGMGGLGKTALAQSVYNNENITKHFDLKLWVCVSEDFDIKV 240
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
IVEKIIESATRKKP+ LQMD LQS+LR I GKKYLLIMDDVWNED+EKWVNLKRLLMGG
Sbjct: 241 IVEKIIESATRKKPEILQMDSLQSDLRTHINGKKYLLIMDDVWNEDYEKWVNLKRLLMGG 300
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
GSRILITTRH+RVAE FD S Y L LD++SAWLLF+KMA G EE KELENSNLV+
Sbjct: 301 TSGSRILITTRHRRVAETFDTTSCYDLGELDDKSAWLLFKKMALGSEEPGKELENSNLVD 360
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIVVKL+G+PLAIRTIGRLLY KK E HLWL+FKDKEL RV+EQREET+F+ SILEL
Sbjct: 361 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWLTFKDKELSRVLEQREETQFILSILEL 420
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLPS+LKQCFLYC LFPKDY+I+KDEVIKQW+AQGF+Q+ G I P DIGEDYFMELL
Sbjct: 421 SYHHLPSSLKQCFLYCTLFPKDYNIRKDEVIKQWVAQGFVQSVGVIKPYDIGEDYFMELL 480
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVTRNE G+I++ K+HD M+DLACSIV EYVS+ +N V+ RTRHISI +F
Sbjct: 481 SRSFFQDVTRNEMGDIIKFKIHDLMNDLACSIVKKEYVSMDVNDKVVTGRTRHISIYHNF 540
Query: 541 GIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLS-----LTDVEKL 600
GI+LE L+ L++AKNLRTLII+ TKD QKVL+ IP KFLRLRTLS + + L
Sbjct: 541 GIKLENLKLLYEAKNLRTLIID--APTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVL 600
Query: 601 PESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRY 660
P+SI KLKHLR+L+I +L+FLPNSITKLYNLETL++ C L+ELP+DAKNW+ LRY
Sbjct: 601 PKSIGKLKHLRYLNIC-LYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRY 660
Query: 661 LNLAENFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHM 720
L+L N +I+FLPDSI L NLE LIL DC L ELPKDIK +NLKHLDL GC LTH+
Sbjct: 661 LSLTGNTEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHL 720
Query: 721 PKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQ 780
PKGL EL +LQTM LF + KD G +LSELNRL+KL GSLTI+GL+ CT DL+ + NL+
Sbjct: 721 PKGLGELTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLR 780
Query: 781 LKSGLQKLKLDWSYSEDELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSS 840
LK G Q+LK W+YS+DE ++ LDN+CK VL CLQPHSNV +I ICGY V LC+WLSS
Sbjct: 781 LKLGFQELKFVWNYSKDEHMNIVLDNECKGVLNCLQPHSNVGKIDICGYPEVMLCDWLSS 840
Query: 841 STLVHLVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLET 900
+TL+HLVSIK+S CF+LQ LPQFDQFP LK L L SLPCI+YI Y SSS + PSLE
Sbjct: 841 NTLLHLVSIKLSDCFQLQVLPQFDQFPCLKCLGLKSLPCIEYIDNDGYRSSSIFFPSLEK 900
Query: 901 LSIVNMHNLRGWWKGETSSESSQNN----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDI 960
LSI++MHNL+GWWKGETSSE S NN +T+SCL +L I +CP+LASIPWHAP + L+I
Sbjct: 901 LSIIDMHNLKGWWKGETSSEGSPNNASFPITMSCLCELKIDDCPQLASIPWHAPLKLLEI 960
Query: 961 HSVSSQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQ 1020
+VS QLL+++++ T + S+LS + LS+I+D+EFLP+E FYHFTNL L I +CK+LQ
Sbjct: 961 CAVSLQLLNVIIEKTANPS-SKLSHVYLSKIDDIEFLPRE-FYHFTNLLFLVIEDCKNLQ 1020
Query: 1021 MSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPN 1080
+SS+LVQ +DVLWKEF+SL L L+ IPKL+YLPNG+QHV LQ I I SCPN
Sbjct: 1021 LSSALVQ-----HSTNDVLWKEFQSLHVLFLRAIPKLEYLPNGMQHVTTLQHIGINSCPN 1080
Query: 1081 LITLPQWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCPKLKERCKEGKGED 1140
+TLP+WI +LTSLS L I NCPKLTSLPE I HL SL+ LTIW+ PKL ER K+GKGE+
Sbjct: 1081 FVTLPKWIHNLTSLSSLSIFNCPKLTSLPEGIHHLRSLESLTIWNSPKLIERYKKGKGEE 1140
Query: 1141 WPKISHVREVEI 1142
W KISH+ + +
Sbjct: 1141 WQKISHIPHINL 1142
BLAST of Lag0026651 vs. ExPASy TrEMBL
Match:
A0A6J1FNX2 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111445964 PE=4 SV=1)
HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 783/1258 (62.24%), Postives = 911/1258 (72.42%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL +VA SLVTKLG A+ E QL W +L+KLK TI AMEAVLHDAE+QQ SH
Sbjct: 54 MAESILFNVAASLVTKLGSPAITEFQLFWSFHDDLDKLKQTILAMEAVLHDAEKQQSNSH 113
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VKNWIS++++AFYDV+DLIDE++YETL+ QVMA K KEVRKVFS P QI FR +M
Sbjct: 114 AVKNWISRVKEAFYDVDDLIDEYTYETLRLQVMADGKRWTKEVRKVFSLPRQIEFRFQMS 173
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
+IK+ REKLKAID DKNQFH SE MI++RDDELR+RRE+SSFI DD+V+GRD DKKAII
Sbjct: 174 HKIKDIREKLKAIDDDKNQFHLSECMINTRDDELRKRRETSSFICDDDVVGRDGDKKAII 233
Query: 181 DLLLDTNV-VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
D LL+T VKEN AVIAIVG+GGLGKTALAQSVYNNE I KHFEL LWVCIS+DFDVKV
Sbjct: 234 DYLLNTYPNVKENVAVIAIVGMGGLGKTALAQSVYNNEGIRKHFELTLWVCISDDFDVKV 293
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
IVEKI+ESAT+ K + LQMDLLQSELR+ I GKKYLLIMDDVWNED+EKWV LKRLLMGG
Sbjct: 294 IVEKILESATKHKSEILQMDLLQSELRKNIDGKKYLLIMDDVWNEDYEKWVTLKRLLMGG 353
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
A GSRILITTRH+RVAE FD S Y L LD+++AWLLF+KMAFGGEE +KE LV+
Sbjct: 354 ASGSRILITTRHRRVAETFDTTSYYALGELDDKNAWLLFKKMAFGGEEPKKE-----LVD 413
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIVVKL+G+PLAIRTIGRLLY KK E HLW +FK+ EL RV+EQREET+FM SILEL
Sbjct: 414 IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWSTFKENELSRVLEQREETQFMLSILEL 473
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLPSNLKQCFLYCALFPKDYDIQKDEVI+QWMA GF+Q+ GTI PNDIGEDYFMELL
Sbjct: 474 SYNHLPSNLKQCFLYCALFPKDYDIQKDEVIRQWMAHGFVQSTGTIKPNDIGEDYFMELL 533
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVTRNE G I+ CKMHD +HDLACSIV +EYVS+ + ++ RTRHI I F
Sbjct: 534 SRSFFQDVTRNEMGIIIYCKMHDLIHDLACSIVEDEYVSMDVKDKLVTERTRHILILKEF 593
Query: 541 GIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFLRLRTLSL----TDVEK-L 600
GI+LE L+ LFKAKNL+TLII+ +D QKV++ I +FL LRTL + T+ K L
Sbjct: 594 GIKLESLKLLFKAKNLKTLIIDAV--NEDCQKVVNLISTQFLCLRTLKVEFCYTNTSKVL 653
Query: 601 PESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCS----------------- 660
P SI KLKHLR+L I LEFLPNSITKLYNLE L++ C
Sbjct: 654 PSSIGKLKHLRYLRICQR-KLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLRY 713
Query: 661 ------------------------------------------------------------ 720
Sbjct: 714 LSLDRNVNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKFL 773
Query: 721 --------------------LEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNLEA 780
L+ELP++AKNW+ LRYL+L N +I+FLPD I L+NLE
Sbjct: 774 PDSIGALHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLET 833
Query: 781 LILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGY 840
LIL DC L ELPKDIK +NLKHL+LSGCT+LTHMPKGL EL SLQTM LF +KKD G
Sbjct: 834 LILQDCSELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGC 893
Query: 841 ELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSEDELVSTAL 900
+LSELNRL+KL GSLTI+GL+ CT LEK +L LK G+Q+L+L W+YSED+ ++ L
Sbjct: 894 DLSELNRLDKLEGSLTIEGLEVCTFDVLEK---SLPLKLGVQELELVWNYSEDQDMNIVL 953
Query: 901 DNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQFD 960
DN+CK + LQPHSN+R I ICGY VRLC+WLSS+T VHLVSI++S CFKLQ LPQFD
Sbjct: 954 DNECKGGFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFD 1013
Query: 961 QFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLETLSIVNMHNLRGWWKGETSSESSQN 1020
QFPFLKYL L SLP I+YI +Y SSS + PSLE LSIV M NL+GWWKGETS ESS N
Sbjct: 1014 QFPFLKYLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPN 1073
Query: 1021 N----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTTPSELS 1080
N + +S L +L I CP LAS PWHA +SL +H++SSQLL++V + T E
Sbjct: 1074 NASFPIAMSRLCKLEIDACPHLASFPWHALLKSLSLHAISSQLLNVVFERTANHCVEECF 1133
Query: 1081 SLCLS--EIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQ-------QHPITEDD 1140
S +S +I+DLEFLP+ FYHFTNL+ LEI +CK+LQMSS+LVQ QHPI
Sbjct: 1134 STFVSFDKIDDLEFLPEGFFYHFTNLEDLEIKDCKNLQMSSALVQHPINSLVQHPI---- 1193
Query: 1141 DDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITLPQWISDLTSLS 1143
+DVLWKEFRSLR L L+ IPKL+YLPNG+QHV L+ I I SCPNLITLP+WI D TSL
Sbjct: 1194 NDVLWKEFRSLRLLYLEAIPKLEYLPNGMQHVTTLERISIISCPNLITLPEWIGDFTSLL 1253
BLAST of Lag0026651 vs. ExPASy TrEMBL
Match:
A0A6J1DKY9 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111021874 PE=4 SV=1)
HSP 1 Score: 1065.4 bits (2754), Expect = 1.6e-307
Identity = 623/1230 (50.65%), Postives = 784/1230 (63.74%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MA+SIL ++A +L+ KLG +E+ LL GV+ EL+KLKGT+ A+ VL DAEE+ KSH
Sbjct: 1 MADSILFNLAGTLINKLGXYVHQELGLLLGVNGELDKLKGTVSAIXKVLLDAEERSSKSH 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
+++W+SKL+ FY+++DLIDEF+YE L++QVM +++ K+VR FSK NQIAF KM
Sbjct: 61 ALEDWLSKLKGVFYEIDDLIDEFNYEALRRQVMTRNRRKTKQVRIFFSKSNQIAFSHKMG 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRD-DELRRRRESSSFIYDDEVIGRDDDKKAI 180
+IKE REKLK I+ DK QF SE +I+ D D LR R+ +I ++EVIGR+ +KK I
Sbjct: 121 GKIKEIREKLKVINDDKTQFKLSECVINKSDVDILRNTRDPGDYIPEEEVIGRNYEKKEI 180
Query: 181 IDLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKV 240
IDLLL++N +E+ AV+AIVG+GGLGKT +AQSVY+++ +TKHFEL+LWVCISE+F+VKV
Sbjct: 181 IDLLLNSN-AREDIAVVAIVGMGGLGKTTIAQSVYSDKMMTKHFELRLWVCISEEFEVKV 240
Query: 241 IVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGG 300
I++ IIE ATR+K QM LLQ+ELR+ + GKKYLL+MDDVWNEDHEKW LKRLLMGG
Sbjct: 241 IIQNIIEYATRRKESFPQMALLQTELRKLVDGKKYLLVMDDVWNEDHEKWNTLKRLLMGG 300
Query: 301 AKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVE 360
AKGSRILITTR +VA FD S Y L L+++++WLLF KMAFGGE +EK+ ENS LVE
Sbjct: 301 AKGSRILITTRTHQVANTFDSTSXYSLEELNDKNSWLLFEKMAFGGEGAEKDPENSKLVE 360
Query: 361 IGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMRSILEL 420
IGKEIV KL+GIPLAIRT+GRLLY KKS W SFKDK+LY++IEQ K M SIL L
Sbjct: 361 IGKEIVTKLKGIPLAIRTVGRLLYYKKST-EFWSSFKDKKLYQIIEQ-GSLKDMLSILML 420
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELL 480
SY HLP NLKQCFLYCA+FPKDY+IQK+EVI QW+AQ FI N DIGE+YFMELL
Sbjct: 421 SYNHLPYNLKQCFLYCAMFPKDYEIQKNEVIMQWIAQSFIHPNDKYKFIDIGEEYFMELL 480
Query: 481 SRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISIDLSF 540
SRSFFQDVT+N+ G+I+ CKMHD MHDLACSIV NE V +N ++ RT H+S DL
Sbjct: 481 SRSFFQDVTKNDLGDIITCKMHDLMHDLACSIVENECVH-DVNARTLSKRTHHVSFDLMS 540
Query: 541 GIELELL-ESLFKAKNLRTLII--EEALSTKDIQKVLDNIPNKFLRLRTLSLTDVE---- 600
ELE L E+L +AKNLRT + + + ++ +++ LRLRTL L +
Sbjct: 541 WEELERLPETLSEAKNLRTCFFHYNQRRTDNNFNEIFESVFYNLLRLRTLDLQLLSFDKQ 600
Query: 601 --KLPESICKLKHLRFLSIS---------------------------------------- 660
K + I KLKHLR+L I
Sbjct: 601 NVKFLKCISKLKHLRYLEIQMYTKTEFFMNHITGLYNLEVFILRCTRLKELPRDIKNWNE 660
Query: 661 -------DSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRYLNLAENFDI 720
+ +EFLPNS +L NLETL++ C+ L+EL D +NWINLRYL+L+ + +
Sbjct: 661 LKHVYLYKNEIIEFLPNSFAELNNLETLVLHQCTKLKELLGDVRNWINLRYLDLSSS-GV 720
Query: 721 EFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPS 780
EFLPDSIT+L NLE L+L C L ELP+D KN INLKH+DL GC LTHMPKGL EL
Sbjct: 721 EFLPDSITQLKNLETLLLRFCSKLQELPRDTKNLINLKHVDLYGCWALTHMPKGLGELTD 780
Query: 781 LQTMTLFSIKKD-SGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKL 840
LQ + F ++KD +G LSELN LNKL GSLTI L +CT DL N+ L LKS LQ+L
Sbjct: 781 LQILNFFVLEKDNNGGGLSELNELNKLRGSLTINRLNFCTTSDLPTNAKFLLLKSSLQEL 840
Query: 841 KLDWSYSEDELVSTA---LDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVH 900
L+W + D + LD+DC+ VLECL PHSN+R + I Y GV+LCNW+SS+ L
Sbjct: 841 VLEWEWDNDSVNEPTIGDLDDDCESVLECLHPHSNLRNLCIDKYRGVKLCNWVSSNFLGC 900
Query: 901 LVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLETLSIVN 960
LV+IK+S C +LQHLPQFDQFP LK L L L I+YI DY SS PSLE LSI
Sbjct: 901 LVTIKLSGCKRLQHLPQFDQFPNLKRLSLRELSGIEYIFNDDYRCSSTLFPSLEELSIFC 960
Query: 961 MHNLRGWW-KGETSSESSQNN----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHSVS 1020
M NL+ WW KG+TS +S N + CLS+L I CP LASI HAP +SL+++
Sbjct: 961 MPNLKRWWKKGQTSKATSSQNVLFPIIFPCLSKLEIWGCPLLASITCHAPPKSLEMN--- 1020
Query: 1021 SQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSS 1080
+ +EF HF
Sbjct: 1021 ---------------------------DPIEF-------HFD------------------ 1080
Query: 1081 LVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHVIALQGIVIGSCPNLITL 1140
++ D V WK F SL YLRL I + +L N LQHV LQ + + C NL L
Sbjct: 1081 -------PDESDVVSWKCFESLSYLRLTHIDEWGFLSNWLQHVTTLQELTLEYCQNLTNL 1140
BLAST of Lag0026651 vs. ExPASy TrEMBL
Match:
A0A6J1DHD4 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020497 PE=4 SV=1)
HSP 1 Score: 987.3 bits (2551), Expect = 5.5e-284
Identity = 609/1328 (45.86%), Postives = 779/1328 (58.66%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESIL +A +++T L A +E+ LL GV EL+KL+ + ++AV+ DAEEQQ+K H
Sbjct: 1 MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDK--TLAKEVRKVFSKPNQIAFRLK 120
K+WISKL+DA YD+ DL DE SYE L++Q +K+ L +VR FS+ N IAF LK
Sbjct: 61 AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQVRMFFSESNPIAFALK 120
Query: 121 MIRRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKA 180
+IKE RE LKAID DK QF+FSER+ ++ +D LR RE +S I ++EVIGR+DDKKA
Sbjct: 121 TGHKIKEIRENLKAIDTDKTQFNFSERVSNTTNDGLRISREDASLILEEEVIGRNDDKKA 180
Query: 181 IIDLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVK 240
II+LLL N +E+ AVI+IVG GGLGKTALAQSVYN+ + KHF+ K WVC+S++F++K
Sbjct: 181 IINLLLKPN-TEEDIAVISIVGTGGLGKTALAQSVYNDNMMDKHFKKKFWVCVSDEFELK 240
Query: 241 VIVEKIIESATRKKPQT-LQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLM 300
V++EK+IESAT K+P + LQMD QSELR+ I GKKYLLIMDDVWNED+EKWV L+RLL+
Sbjct: 241 VMIEKMIESATGKRPDSFLQMDSSQSELRKLIDGKKYLLIMDDVWNEDYEKWVTLRRLLI 300
Query: 301 GGAKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNL 360
GAKGSRILITTR ++VA+ FD SLY L LD +WLLF+KMAF E++ ENSNL
Sbjct: 301 CGAKGSRILITTRSEKVAKTFDTSSLYSLEKLDEYHSWLLFKKMAFLKGSEEQDTENSNL 360
Query: 361 VEIGKEIVVKLRGIPLAIRTIGRLL-YDKKSEFHLWLSFKDKELYRVIEQREETKFMRSI 420
V+IGKEIV KL+G+PLAIRTIGRLL Y+KKS F W SFKDKELY++IE+ E K M SI
Sbjct: 361 VQIGKEIVAKLKGVPLAIRTIGRLLCYNKKSNF--WSSFKDKELYQIIEE-ESVKKMLSI 420
Query: 421 LELSYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGPNDIGEDYFM 480
L+LSY LPSNLKQCFLYCALFPKDY I+K+E++ QW+AQGF+Q+N + D GEDYFM
Sbjct: 421 LKLSYNSLPSNLKQCFLYCALFPKDYKIRKEEIVLQWIAQGFVQSNDGVKALDAGEDYFM 480
Query: 481 ELLSRSFFQDVTRNETGNIVECKMHDFMHDLACSIV-GNEYVSIGMNHMVINNRTRHISI 540
+LLS SFFQDV +N+ G+I+ CKMHD MHDLACSIV NE + +N +N RT H+S
Sbjct: 481 KLLSMSFFQDVRKNDVGDIITCKMHDLMHDLACSIVENNEIIVHDVNARTLNKRTHHVSF 540
Query: 541 DLSFGIELELL-ESLFKAKNLRTLII--------EEALSTKDIQKVLDNIPNKFLRLRTL 600
L G ELE L ++L KA+NLRT + ++ L + I KV ++ LRLRTL
Sbjct: 541 VLLSGKELEKLPKTLSKARNLRTCFLQYNQVGRRDDMLGKRKINKVFGSVFYNLLRLRTL 600
Query: 601 SL---------------------------------------------------------- 660
L
Sbjct: 601 DLHLSIDNHNVKFLKCISKLKYLRYLKIKMIETTEYFPNSITGLYSLEVFILECKGLKEL 660
Query: 661 ------------------------------------------------------------ 720
Sbjct: 661 PRDIKNWNKLKHLYLDGNENVWHRQDTYHYGNENVEFLPSSTVVLNNLETLVLQNFNNLK 720
Query: 721 --------------------------------------------TDVEKLPESICKLKHL 780
V +LP+ I L HL
Sbjct: 721 EFPEDIKNLTNLTHLVMRGNKNIEYLPNSMSELNNLKSLILTRFEKVRELPQDIKNLIHL 780
Query: 781 RFLSISDSIDLEFLPNSITKLYNLETLMVIDCS-LEELPKDAKNWINLRYLNLAENFDIE 840
+ L + + ++EFLPNSIT+L LET+++ C+ L+ELP+D KNWINLR+L+L+ + +E
Sbjct: 781 QHLDLQWNRNVEFLPNSITELCELETIILKGCTKLKELPRDVKNWINLRHLDLSGS-GVE 840
Query: 841 FLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSL 900
FLPDSIT NLE L+L C L ELP D K INLKHLDLSGC LTHMPKGL EL +L
Sbjct: 841 FLPDSITHSNNLETLLLRYCEKLKELPGDTKKLINLKHLDLSGCRALTHMPKGLGELTNL 900
Query: 901 QTMTLFSIKK--DSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKL 960
QT+ LF + K ++G LSELN LNKL GSL I+GL++C+A DL+ N+ LQLKSGLQ+L
Sbjct: 901 QTLNLFILGKGENNGGGLSELNGLNKLRGSLIIKGLQFCSAVDLQTNAKLLQLKSGLQQL 960
Query: 961 KLDWSYSED---ELVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVH 1020
L+W + +D E ALD + +RVLECLQPHSN+ IHI GY GV+LCNWLSSS+
Sbjct: 961 MLEWKWDKDSFNEPTVGALDGEYERVLECLQPHSNLPEIHINGYRGVKLCNWLSSSSYSF 1020
Query: 1021 --LVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSSKYLPSLETLSI 1080
LV + +S C +LQHLP+F+QFP LK+L L LPC+ ++V DY SS S E L +
Sbjct: 1021 DGLVRMTVSSCERLQHLPRFEQFPNLKHLHLRDLPCVRHMVNNDYPSSPTLFQSPEVLVV 1080
Query: 1081 VNMHNL-RGWWKGETSSESSQNN----LTVSCLSQLVIRNCPRLASIPWHAPSQSLDIHS 1140
+M NL RGW KG ++S+QN + CLS+L I CP LAS
Sbjct: 1081 SDMPNLKRGWKKG---TKSAQNGVLFPIIFPCLSELCIWKCPLLAS-------------- 1140
BLAST of Lag0026651 vs. ExPASy TrEMBL
Match:
A0A5D3CGC1 (Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00530 PE=4 SV=1)
HSP 1 Score: 984.9 bits (2545), Expect = 2.7e-283
Identity = 577/1142 (50.53%), Postives = 761/1142 (66.64%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSH 60
MAESI+ +VA +++T +G LALRE+ L WG++ EL+KL+ T+ ++ AVL DAEEQQ KSH
Sbjct: 1 MAESIIFAVAGNMITNIGSLALRELGLSWGINDELDKLQDTLSSIRAVLLDAEEQQSKSH 60
Query: 61 TVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMI 120
VK+WISKL+D FYDV+DL+DE SYETLK+QV+ + + K VR SK ++I+F LKM
Sbjct: 61 AVKDWISKLRDVFYDVDDLVDELSYETLKKQVLTESRRTTKHVRIFLSKFHRISFALKMG 120
Query: 121 RRIKETREKLKAIDADKNQFHFSERMISSRDDELRRRRESSSFIYDDEVIGRDDDKKAII 180
++IKE REKL AI DK++ H S RM + DELR+ RE+SSFI + EVIGRDDDKKAII
Sbjct: 121 QQIKEVREKLNAISDDKDKLHLSMRMGEIQGDELRKIRETSSFIPEGEVIGRDDDKKAII 180
Query: 181 DLLLDTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVI 240
D LLDT K+N VI+IVG+GGLGKTALAQS++N+EKI KHF+LKLWVCISE+F+V+ I
Sbjct: 181 DFLLDTKTKKDNVEVISIVGMGGLGKTALAQSIFNDEKINKHFQLKLWVCISEEFNVRTI 240
Query: 241 VEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGA 300
V IIE KK + LQ+D LQS LRE+I GK+YLL+MDDVWNE HEKW++LKR LMGGA
Sbjct: 241 VGNIIELFEEKKLEPLQLDKLQSMLREKINGKRYLLVMDDVWNESHEKWIDLKRYLMGGA 300
Query: 301 KGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNLVEI 360
GSRILITTR Q+VA+ D +S +HL LDN ++W+LFRKMAF E E+E+EN NLV+I
Sbjct: 301 MGSRILITTRSQQVAQTSDTVSFHHLKELDNHNSWVLFRKMAFLNE--EEEIENLNLVKI 360
Query: 361 GKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDK-ELYRVIEQREETKFMRSILEL 420
GKEIV KL+G PL IR +GRLLY + +E WLSFKD+ EL ++Q + ++ IL++
Sbjct: 361 GKEIVAKLKGSPLGIRVVGRLLYFRNTEKD-WLSFKDRNELGTTLQQENQ---IQQILKI 420
Query: 421 SYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQG-FIQTNGTIGPNDIGEDYFMEL 480
S+ HLPSNLK+CF+YC+LFPKDY+ +KDE++K WMAQG IQ + D+G+DYF EL
Sbjct: 421 SFDHLPSNLKRCFMYCSLFPKDYEFRKDELVKLWMAQGLIIQPHNEKAIEDVGDDYFKEL 480
Query: 481 LSRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHMVINNRTRHISI--D 540
+ RSFFQD+ +N+ G+I +CKMHD MHDLA +V NE V + I+ RTRH+S +
Sbjct: 481 VGRSFFQDIRKNKWGDIKKCKMHDLMHDLALLMVENECVVVNDGVGSIDKRTRHVSFLYN 540
Query: 541 LSFGIELELL-ESLFKAKNLRT--LIIEEALSTKDIQKVLDNIPNKFLRLRTLSLTDVEK 600
I EL+ S KAK LRT L I S K ++ RLR L+L
Sbjct: 541 RRRPISRELVPRSFTKAKKLRTLNLTINSISSLKTFYRI------NLFRLRALNLDRCCH 600
Query: 601 LPESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCSLEELPKDAKNWINLRY 660
P+ I LKHLR+L NL Y
Sbjct: 601 PPKFIDTLKHLRYL------------------------------------------NLSY 660
Query: 661 LNLAENFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSGCTDLTHM 720
L+ I+FLP IT+LYNLE LILH C L ELPKDI N +NL+H+DL GC LTHM
Sbjct: 661 LS------IDFLPKFITKLYNLETLILHYCCWLRELPKDISNLMNLRHIDLHGCFRLTHM 720
Query: 721 PKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQ 780
PKGL ++ SLQTM LF + KD G LSELN L L GSL ++GL++CT D+E N +
Sbjct: 721 PKGLGKMSSLQTMNLFVLGKDKGGNLSELNGLKSLRGSLCVRGLQFCTTIDIE-NVKYFE 780
Query: 781 LKSGLQKLKLDWSYSEDE-LVSTALDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLS 840
KS ++KL+L W + + + A + +R LECL+PHSNV ++ I GY G++LC+W+S
Sbjct: 781 EKSEIRKLELHWDTDKKKPRIDDASYAEDERTLECLKPHSNVGKMSIKGYRGMKLCDWVS 840
Query: 841 SSTLVH-LVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYLSSS-KYLPS 900
S + LVSI++ C KL+HLPQFDQFP+LK L L L I+YI + +SSS + PS
Sbjct: 841 SDYFLDGLVSIELCHCEKLEHLPQFDQFPYLKNLHLEDLTNIEYIDDSNSVSSSTTFFPS 900
Query: 901 LETLSIVNMHNLRGWW-KGE-TSSESSQNNLTVSC----LSQLVIRNCPRLASIPWHAPS 960
LE L I M L+GWW +GE S+ S+Q N ++ LSQL I +CP+LA IP H P
Sbjct: 901 LEKLRIKKMPKLKGWWRRGEIPSNYSAQYNASLPTALHQLSQLWILDCPQLAFIPQHPPL 960
Query: 961 QSLDIHSVSSQLLDMVMKATT--------TTTPSELSSLCLSEIEDLEFLPKELFYHFTN 1020
QSL I V ++ DMV++ T ++T S+LS+L + I D++FLP+ L + +
Sbjct: 961 QSLAIGGVGLKVFDMVIRMATILAADSSSSSTLSKLSTLRIENI-DIKFLPEALNCNMKD 1020
Query: 1021 LKHLEIINCKSLQMSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLPNGLQHV 1080
L+ L I +CK LQMSSS + E+D V WKE +LR L ++IPKL+YLP GL+++
Sbjct: 1021 LESLTIRSCKYLQMSSSRLVYE---EEDRLVYWKELSNLRSLCFRDIPKLEYLPKGLEYM 1076
Query: 1081 IALQGIVIGSCPNLITLPQWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQLLTIWDCP 1119
AL+ + + C NL+++ + I L+SLS +I +CPKL LPEEI HL SL LTI++CP
Sbjct: 1081 TALESLDLMYCENLVSI-EGIGQLSSLSSFYIHSCPKLRLLPEEIGHLISLSDLTIFNCP 1076
BLAST of Lag0026651 vs. TAIR 10
Match:
AT3G14470.1 (NB-ARC domain-containing disease resistance protein )
HSP 1 Score: 398.3 bits (1022), Expect = 2.1e-110
Identity = 329/1055 (31.18%), Postives = 522/1055 (49.48%), Query Frame = 0
Query: 35 LEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMA 94
LE+L + + AVL DAEE+Q + V+ W+++L+D Y ED +D+ + E L+ + A
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KDKTLAKEVRKVFSKPNQIAF----RLKMIRRIKETREKLKAIDADKNQFHFSE--RMIS 154
+ + + +R++ + + F + R+++ +L+ + + +N E MI
Sbjct: 99 ESSS-SNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIP 158
Query: 155 SRDDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNVVKENTAVIAIVGIGGLGKTA 214
++R ++S + + EV GRDDDK I+ L+ N V+AIVGIGG+GKT
Sbjct: 159 ------KQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTT 218
Query: 215 LAQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDLLQSELREQ 274
L+Q +YN++ + +F K+W +SE+FDV I +K+ ES T + + +D+LQ +L+E+
Sbjct: 219 LSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKER 278
Query: 275 IVGK--KYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENFDPISLYHL 334
+ G +LL++DD+WNE+ W L++ + A+GS+IL+TTR QRVA + +++L
Sbjct: 279 LTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNL 338
Query: 335 ANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRLL--YD 394
L + W LF K FG +E N + ++ + IV K RG+PLA++T+G +L
Sbjct: 339 QPLSDGDCWSLFMKTVFGNQE---PCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEG 398
Query: 395 KKSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKDYDI 454
K E+ LS R+ + + + +L +SY +LP++LK+CF YC++FPK +
Sbjct: 399 KVIEWERVLS------SRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAF 458
Query: 455 QKDEVIKQWMAQGFI-QTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMHDF 514
+KD+V+ WMA+GF+ QT + ++G +YF EL SRS Q + +T I MHDF
Sbjct: 459 EKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQ---KTKTRYI----MHDF 518
Query: 515 MHDLACSIVGNEYVSIGMN--HMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIE 574
+++LA E+ S + + ++ RTR++S E E+L + K LRT +
Sbjct: 519 INELA-QFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFL-- 578
Query: 575 EALSTKDIQKVLDNIPNKFLRLRTLSLTDVEKLPESICKLKHLRFLSISDSIDLEFLPNS 634
LSLT+ + S C + + + LP
Sbjct: 579 -----------------------PLSLTNSSR---SCC---------LDQMVSEKLLPT- 638
Query: 635 ITKLYNLETLMVIDCSLEELPKD-AKNWINLRYLNLAENFDIEFLPDSITELYNLEALIL 694
L L L + + LP D KN + R+L+L+ ++E LP S+ +YNL+ L+L
Sbjct: 639 ---LTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLL 698
Query: 695 HDCISLIELPKDIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGYELS 754
C SL ELP DI N INL++LDL G T L MP+ L SLQT+T F + G +S
Sbjct: 699 SYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRIS 758
Query: 755 ELNRLNKLTGSLTIQGLKYCTAHDLEKNSINLQLKSGLQKLKLDW----SYSEDELVSTA 814
EL L+ L G L I L+ + NL K L+++ W S SE+
Sbjct: 759 ELGGLHDLHGKLKIVELQR-VVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHR 818
Query: 815 LDNDCKRVLECLQPHSNVRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQF 874
N+ + V E L+PH ++ ++ I Y G R +WLS + +V I++ C LP
Sbjct: 819 TQNEAE-VFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSL 878
Query: 875 DQFPFLKYLCLTSLPCIDYIVKKDYLSSSKY-------LPSLETLSIVNMHNLRGWWKGE 934
Q P LK L ++ + + I +K Y S + SLETL N+ + + W
Sbjct: 879 GQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEW---- 938
Query: 935 TSSESSQNNLTVSCLSQLVIRNCPRL-ASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTT 994
++ +L S L +L I CP L ++P PS + +H LLD
Sbjct: 939 LDVRVTRGDLFPS-LKKLFILRCPELTGTLPTFLPS-LISLHIYKCGLLDF---QPDHHE 998
Query: 995 PSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEIINCKSLQMSSSLVQQHPITEDDDDVL 1054
S + LS + L K HF NL LE+ C SL S L +H
Sbjct: 999 YSYRNLQTLSIKSSCDTLVKFPLNHFANLDKLEVDQCTSL-YSLELSNEH---------- 1004
Query: 1055 WKEFRSLRYLRLKE------IPKLKYLPNGLQHVI 1058
+ +LR LR+ + +PKL LP LQ I
Sbjct: 1059 LRGPNALRNLRINDCQNLQLLPKLNALPQNLQVTI 1004
BLAST of Lag0026651 vs. TAIR 10
Match:
AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )
HSP 1 Score: 337.8 bits (865), Expect = 3.4e-92
Identity = 278/954 (29.14%), Postives = 462/954 (48.43%), Query Frame = 0
Query: 1 MAESILLSVATSLVTKLGYLALREVQLLWG--VDAELEKLKGTIEAMEAVLHDAEEQQFK 60
MA S L S A +V ++ + V+L G A L++LK + VL DA+++
Sbjct: 1 MANSYLSSCANVMVERIN-TSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEH 60
Query: 61 SHTVKNWISKLQDAFYDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRL- 120
VK+W++ ++DAF+ ED++DE E L+++V+A+ L + + + I ++
Sbjct: 61 VREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIE 120
Query: 121 -KMIRRIKETREKLKAIDADKNQFHFSERMISSRDDELRR-RRESSSFIYDDEVIGRDDD 180
KM + ++ +K I+ + +SE +R+ + R+ R + ++GR +D
Sbjct: 121 PKMEKVVRLLEHHVKHIEVIGLK-EYSE----TREPQWRQASRSRPDDLPQGRLVGRVED 180
Query: 181 KKAIIDLLL-DTNVVKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISED 240
K A+++LLL D + AVI++VG+ G+GKT L + V+N+ ++T+HFE+K+W+ +
Sbjct: 181 KLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGIN 240
Query: 241 FDVKVIVEKIIESATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKR 300
F+V + + +++ T T + LQ +L++ + GK++LL++DD W+E +W + +
Sbjct: 241 FNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQV 300
Query: 301 LLMGGAKGSRILITTRHQRVAENFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELEN 360
+GS+I++TTR + V+ +Y + + NE W L + AFG N
Sbjct: 301 AFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGS--IN 360
Query: 361 SNLVEIGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQREETKFMR 420
L IGK I + +G+PLA R I L K + + K+ Y T +
Sbjct: 361 QELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSSY--------TNSIL 420
Query: 421 SILELSYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFI-QTNGTIGPNDIGED 480
+L+LSY LP LK+CF C++FPK + ++E++ WMA + Q + DIG D
Sbjct: 421 PVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGND 480
Query: 481 YFMELLSRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNEYVSIGMNHM-VINNRTRH 540
Y +L+++SFFQ + T + MHD M+DLA ++ G+ + +++ I + TRH
Sbjct: 481 YLGDLVAQSFFQRLDITMTSFV----MHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRH 540
Query: 541 ISIDLSFGIELELLESLFKAKNLRTLI-IEEALSTKDIQ---KVLDNIPNKFLRLRTLSL 600
S S S+ A+ LRT++ S + +Q KVL+ + N LR LSL
Sbjct: 541 FSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSL 600
Query: 601 T--DVEKLPESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVIDCSLEELPKDAK 660
+ + LP+S+ LK LR+L +S +
Sbjct: 601 SHYQITNLPKSLKGLKLLRYLDLSST---------------------------------- 660
Query: 661 NWINLRYLNLAENFDIEFLPDSITELYNLEALILHDCISLIELPKDIKNWINLKHLDLSG 720
I+ LP+ + L NL+ L+L +C L LPK I INL+ LDL G
Sbjct: 661 --------------KIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG 720
Query: 721 CTDLTHMPKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLTIQGLKYCTAHDLE 780
T L MP G+ +L SLQ ++ F I + SG L EL L+ L G+L I L+ A E
Sbjct: 721 -TPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQN-VAFASE 780
Query: 781 KNSINLQLKSGLQKLKLDWSYSEDELVS---TALDNDCKRVLECLQPHSNVRRIHICGYL 840
L+ K L L L W+ V AL D K VL L+PH +++ I Y
Sbjct: 781 AKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQ 840
Query: 841 GVRLCNWLSSSTLVHLVSIKISRCFKLQHLPQFDQFPFLKYLCLTSLPCIDYIVKKDYL- 900
G WL S+ + S+ +S C LP Q P LKYL + + + +
Sbjct: 841 GGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFG 880
Query: 901 -SSSKYLPSLETLSIVNMHNLRGWWKGETSSESSQNNLTVSCLSQLVIRNCPRL 936
++S+ +P ++L I+ + + W E ++ + CL +L+I+ CP L
Sbjct: 901 ENNSRGVP-FQSLQILKFYGMPRW--DEWICPELEDGI-FPCLQKLIIQRCPSL 880
HSP 2 Score: 56.6 bits (135), Expect = 1.5e-07
Identity = 56/191 (29.32%), Postives = 81/191 (42.41%), Query Frame = 0
Query: 943 PSQSLDIHSVSSQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFYHFTNLKHLEI 1002
P +H S L + + T + P+ L L + LE P + T LK L I
Sbjct: 1090 PQNLQSLHIDSCDGLTSLPENLTESYPN-LHELLIIACHSLESFPGS--HPPTTLKTLYI 1149
Query: 1003 INCKSLQMSSSLVQQHPITEDDDDVLWKEFRSLRYLRLKEIPKLKYLP------------ 1062
+CK L + SL ++ + + +L L PKL+ L
Sbjct: 1150 RDCKKLNFTESLQPTRSYSQLEYLFIGSSCSNLVNFPLSLFPKLRSLSIRDCESFKTFSI 1209
Query: 1063 -NGL-QHVIALQGIVIGSCPNLITLPQWISDLTSLSRLFIENCPKLTSLPEEIRHLHSLQ 1120
GL IAL+ + I CPNL T PQ LS + + NC KL +LPE++ L SL
Sbjct: 1210 HAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSMLLSNCKKLQALPEKLFGLTSLL 1269
BLAST of Lag0026651 vs. TAIR 10
Match:
AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 252.3 bits (643), Expect = 1.9e-66
Identity = 206/736 (27.99%), Postives = 354/736 (48.10%), Query Frame = 0
Query: 34 ELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEFSYETLKQQVM 93
+LE L+ ++ M++ L DAE Q+ + T++ ++ L++ Y+ ED++ + Q
Sbjct: 30 QLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDC------QLAD 89
Query: 94 AKDKTLAKEVRKVFSK--PNQIAFRLKMIRRIKETREKLKAIDAD-KNQFHFSERMISSR 153
D + S+ P ++ + K +R++E E++ I + + F F R
Sbjct: 90 GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGR 149
Query: 154 DDELRRRRESSSFIYD-DEVIGRDDDKKAIIDLLLDTNVVKENTAVIAIVGIGGLGKTAL 213
D+ R SS +YD +V+G + DK+ I + L +N ++A VG+GGLGKT +
Sbjct: 150 DNGTDR---WSSPVYDHTQVVGLEGDKRKIKEWLFRSN--DSQLLIMAFVGMGGLGKTTI 209
Query: 214 AQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDLLQSELREQI 273
AQ V+N+++I FE ++WV +S+ F + I+ I+ + + L ++++ +
Sbjct: 210 AQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGD-DIGTLLRKIQQYL 269
Query: 274 VGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENF----DPISLYH 333
+GK+YL++MDDVW+++ W + + L G GS +++TTR + VA+ D
Sbjct: 270 LGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS-VIVTTRSESVAKRVQARDDKTHRPE 329
Query: 334 LANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRLLYDK 393
L + DN +WLLF +AF + E L ++GKEIV K +G+PL I+ +G LL K
Sbjct: 330 LLSPDN--SWLLFCNVAFAANDG--TCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCK 389
Query: 394 KSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKDYDIQ 453
+H W + + ET + S L+LSY LPS+LK C L +L+P+D I
Sbjct: 390 DHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIP 449
Query: 454 KDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMHDFMH 513
K +++ W+ +GF+ + GED F L +R + V + +G I+ CK+HD +
Sbjct: 450 KQQLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVR 509
Query: 514 DLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIEEALS 573
DL I + S N +N RH+ I +F E K N + +
Sbjct: 510 DLVIDIAKKDSFS---NPEGLN--CRHLGISGNFD------EKQIKV-NHKLRGVVSTTK 569
Query: 574 TKDIQKVLDNIPNKFLRLRTLSLTDVEK---------LPESICKLKHLRFLSISDSIDLE 633
T ++ K+ ++ KF + L + D+ K + + I L+HL LS+S++ L
Sbjct: 570 TGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLI 629
Query: 634 FLPNSITKLYNLETLMVIDC-SLEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNL 693
P S+ L+NL+ L C +L++L + L L++ +E P I L L
Sbjct: 630 QFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKL 689
Query: 694 EALILHDCISLIELPK--DIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKK 750
E L+ K ++KN NL+ L LS + L L +L + SI
Sbjct: 690 EVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINC 736
BLAST of Lag0026651 vs. TAIR 10
Match:
AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )
HSP 1 Score: 252.3 bits (643), Expect = 1.9e-66
Identity = 206/736 (27.99%), Postives = 354/736 (48.10%), Query Frame = 0
Query: 34 ELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAFYDVEDLIDEFSYETLKQQVM 93
+LE L+ ++ M++ L DAE Q+ + T++ ++ L++ Y+ ED++ + Q
Sbjct: 30 QLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDC------QLAD 89
Query: 94 AKDKTLAKEVRKVFSK--PNQIAFRLKMIRRIKETREKLKAIDAD-KNQFHFSERMISSR 153
D + S+ P ++ + K +R++E E++ I + + F F R
Sbjct: 90 GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGR 149
Query: 154 DDELRRRRESSSFIYD-DEVIGRDDDKKAIIDLLLDTNVVKENTAVIAIVGIGGLGKTAL 213
D+ R SS +YD +V+G + DK+ I + L +N ++A VG+GGLGKT +
Sbjct: 150 DNGTDR---WSSPVYDHTQVVGLEGDKRKIKEWLFRSN--DSQLLIMAFVGMGGLGKTTI 209
Query: 214 AQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIESATRKKPQTLQMDLLQSELREQI 273
AQ V+N+++I FE ++WV +S+ F + I+ I+ + + L ++++ +
Sbjct: 210 AQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGD-DIGTLLRKIQQYL 269
Query: 274 VGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSRILITTRHQRVAENF----DPISLYH 333
+GK+YL++MDDVW+++ W + + L G GS +++TTR + VA+ D
Sbjct: 270 LGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS-VIVTTRSESVAKRVQARDDKTHRPE 329
Query: 334 LANLDNESAWLLFRKMAFGGEESEKELENSNLVEIGKEIVVKLRGIPLAIRTIGRLLYDK 393
L + DN +WLLF +AF + E L ++GKEIV K +G+PL I+ +G LL K
Sbjct: 330 LLSPDN--SWLLFCNVAFAANDG--TCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCK 389
Query: 394 KSEFHLWLSFKDKELYRVIEQREETKFMRSILELSYKHLPSNLKQCFLYCALFPKDYDIQ 453
+H W + + ET + S L+LSY LPS+LK C L +L+P+D I
Sbjct: 390 DHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIP 449
Query: 454 KDEVIKQWMAQGFIQTNGTIGPNDIGEDYFMELLSRSFFQDVTRNETGNIVECKMHDFMH 513
K +++ W+ +GF+ + GED F L +R + V + +G I+ CK+HD +
Sbjct: 450 KQQLVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVR 509
Query: 514 DLACSIVGNEYVSIGMNHMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIEEALS 573
DL I + S N +N RH+ I +F E K N + +
Sbjct: 510 DLVIDIAKKDSFS---NPEGLN--CRHLGISGNFD------EKQIKV-NHKLRGVVSTTK 569
Query: 574 TKDIQKVLDNIPNKFLRLRTLSLTDVEK---------LPESICKLKHLRFLSISDSIDLE 633
T ++ K+ ++ KF + L + D+ K + + I L+HL LS+S++ L
Sbjct: 570 TGEVNKLNSDLAKKFTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLI 629
Query: 634 FLPNSITKLYNLETLMVIDC-SLEELPKDAKNWINLRYLNLAENFDIEFLPDSITELYNL 693
P S+ L+NL+ L C +L++L + L L++ +E P I L L
Sbjct: 630 QFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKL 689
Query: 694 EALILHDCISLIELPK--DIKNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKK 750
E L+ K ++KN NL+ L LS + L L +L + SI
Sbjct: 690 EVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINC 736
BLAST of Lag0026651 vs. TAIR 10
Match:
AT1G58848.1 (Disease resistance protein (CC-NBS-LRR class) family )
HSP 1 Score: 243.4 bits (620), Expect = 8.7e-64
Identity = 300/1178 (25.47%), Postives = 499/1178 (42.36%), Query Frame = 0
Query: 14 VTKLGYLALREVQLLWGVDAELEKLKGTIEAMEAVLHDAEEQQFKSHTVKNWISKLQDAF 73
+ L L +E +L GV+ ++ +LK + + + L DA+ ++ S VKN + ++++
Sbjct: 10 IQNLWNLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKNCVEEIKEII 69
Query: 74 YDVEDLIDEFSYETLKQQVMAKDKTLAKEVRKVFSKPNQIAFRLKMIRRIKETREKLKAI 133
YD ED I+ F E Q + K + K +R++ I R + I ++ +
Sbjct: 70 YDGEDTIETFVLE----QNLGKTSGIKKSIRRLAC---IIPDRRRYALGIGGLSNRISKV 129
Query: 134 DADKNQFHFSERMISS-----RDDELRRRRESSSFIYDDEVIGRDDDKKAIIDLLLDTNV 193
D F + ++ + D+ R R S D + +G + + K ++ L+D
Sbjct: 130 IRDMQSFGVQQAIVDGGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGYLVD--- 189
Query: 194 VKENTAVIAIVGIGGLGKTALAQSVYNNEKITKHFELKLWVCISEDFDVKVIVEKIIE-- 253
+ N V++I G+GGLGKT LA+ V+N+E + F+ WVC+S+DF + +KI+
Sbjct: 190 -EANVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQKILRDL 249
Query: 254 --SATRKKPQTLQMDLLQSELREQIVGKKYLLIMDDVWNEDHEKWVNLKRLLMGGAKGSR 313
KK + D LQ EL + K L+++DD+W + E W L + + KG +
Sbjct: 250 KPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDW-ELIKPIFPPTKGWK 309
Query: 314 ILITTRHQRVAE-------NFDPISLYHLANLDNESAWLLFRKMAFGGEESEKELENSNL 373
+L+T+R++ VA NF P L E +W LF+++A +++ + +
Sbjct: 310 VLLTSRNESVAMRRNTSYINFKP------ECLTTEDSWTLFQRIALPMKDAAEFKIDEEK 369
Query: 374 VEIGKEIVVKLRGIPLAIRTIGRLLYDKKSEFHLWLSFKDKELYRVIEQR-----EETKF 433
E+GK ++ G+PLAIR +G +L +K + H W + ++ R +
Sbjct: 370 EELGKLMIKHCGGLPLAIRVLGGMLAEKYTS-HDWRRLSENIGSHLVGGRTNFNDDNNNT 429
Query: 434 MRSILELSYKHLPSNLKQCFLYCALFPKDYDIQKDEVIKQWMAQGFIQTNGTIGP--NDI 493
+L LS++ LPS LK CFLY A FP DY+I + W A+G Q G D+
Sbjct: 430 CNYVLSLSFEELPSYLKHCFLYLAHFPDDYEINVKNLSYYWAAEGIFQPRHYDGEIIRDV 489
Query: 494 GEDYFMELLSRSFFQDVTRNETGNIVECKMHDFMHDLACSIVGNE---------YVSIGM 553
G+ Y EL+ R+ +T C +HD M ++ C + E S G
Sbjct: 490 GDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREV-CLLKAKEENFLQITSSRTSTGN 549
Query: 554 NHMVINNRTRHISIDLSFGIELELLESLFKAKNLRTLIIEEALSTKDIQKVLDNIPNKFL 613
+ ++ +R ++ +E ++ + LR+L++ + + F+
Sbjct: 550 SLSIVTSRRLVYQYPITLDVEKDIND-----PKLRSLVVVANTYMFWGGWSWMLLGSSFI 609
Query: 614 RLRTLSLTDVE-------KLPESICKLKHLRFLSISDSIDLEFLPNSITKLYNLETLMVI 673
RL L + D+ KL SI +L HLR+L++ + ++ +P S L NL+ L+
Sbjct: 610 RLELLRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHA-EVTHIPYS---LGNLKLLI-- 669
Query: 674 DCSLEELPKDAKNWINLRYLNLAENFD-IEFLPDSITELYNLEALILHDCISLIELPKDI 733
YLNL +P+ + E+ L L LPKD+
Sbjct: 670 ------------------YLNLVILVSGSTLVPNVLKEMQQLRYL---------ALPKDM 729
Query: 734 KNWINLKHLDLSGCTDLTHMPKGLSELPSLQTMTLFSIKKDSGYELSELNRLNKLTGSLT 793
L+ LS L L+T+ FS K S +L + RL LT
Sbjct: 730 GRKTKLE----------------LSNLVKLETLKNFSTKNCSLEDLRGMVRLRTLT---- 789
Query: 794 IQGLKYCTAHDLEKNSINLQLKSGLQKLKLDWSYSEDELVSTALDNDCKRVLECLQPHSN 853
+L K + L + + LK S + +L S + V + + +
Sbjct: 790 ---------IELRKETSLETLAASIGGLKYLESLTITDLGSEMRTKEAGIVFDFVYLKTL 849
Query: 854 VRRIHICGYLGVRLCNWLSSSTLVHLVSIKISRC-FKLQHLPQFDQFPFLKYLCLTSLPC 913
++++ RL HL ++ + C + +P ++ LK L L
Sbjct: 850 TLKLYM-----PRLSK--EQHFPSHLTTLYLQHCRLEEDPMPILEKLHQLKELELRR--- 909
Query: 914 IDYIVKKDYLSSSKYLPSLETLSIVNMHNLRGWWKGETSSESSQNNLTVSCLSQLVIRNC 973
K+ + SS P L+ LSI + WK E SS + L L IR+C
Sbjct: 910 -KSFSGKEMVCSSGGFPQLQKLSIKGLEEWED-WKVEESS--------MPVLHTLDIRDC 969
Query: 974 PRLASIPWHAPSQSLDIHSVSSQLLDMVMKATTTTTPSELSSLCLSEIEDLEFLPKELFY 1033
+L + P + L H S L ++ T L L ++L+ LF
Sbjct: 970 RKLKQL----PDEHLPSHLTSISLFFCCLEEDPMPTLERLVHL-----KELQL----LFR 1029
Query: 1034 HFTNLKHLEIINCKSLQMSSSLVQQHPITEDDDDVL--W----KEFRSLRYLRLKEIPKL 1093
F+ I+ C S Q H + + D L W L L ++ PKL
Sbjct: 1030 SFSG----RIMVC----AGSGFPQLHKLKLSELDGLEEWIVEDGSMPQLHTLEIRRCPKL 1047
Query: 1094 KYLPNGLQHVIALQGIVIGSCPNLITLPQWI---SDLTSLSRLFIENCPKLTSLPEEIRH 1141
K LPNG LQ + + L +WI + L L I NCPKL LP+ +R
Sbjct: 1090 KKLPNGFPQ---LQNLELN---ELEEWEEWIVEDGSMPLLHTLRIWNCPKLKQLPDGLRF 1047
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022931674.1 | 0.0e+00 | 67.97 | putative disease resistance protein RGA1 [Cucurbita moschata] >XP_022931675.1 pu... | [more] |
| XP_023539563.1 | 0.0e+00 | 67.01 | uncharacterized protein LOC111800203 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022940308.1 | 0.0e+00 | 62.24 | putative disease resistance protein RGA4 [Cucurbita moschata] | [more] |
| XP_023547023.1 | 0.0e+00 | 70.54 | disease resistance protein RGA2-like isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023547024.1 | 0.0e+00 | 70.97 | disease resistance protein RGA2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q7XA40 | 9.4e-148 | 32.26 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XA39 | 2.3e-146 | 31.85 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q7XBQ9 | 1.1e-143 | 31.98 | Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... | [more] |
| Q7XA42 | 3.1e-143 | 31.72 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... | [more] |
| Q9LRR4 | 3.0e-109 | 31.18 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1EUW9 | 0.0e+00 | 67.97 | putative disease resistance protein RGA1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| A0A6J1FNX2 | 0.0e+00 | 62.24 | putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| A0A6J1DKY9 | 1.6e-307 | 50.65 | putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
| A0A6J1DHD4 | 5.5e-284 | 45.86 | disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020... | [more] |
| A0A5D3CGC1 | 2.7e-283 | 50.53 | Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... | [more] |
