Lag0026580 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0026580 vs. NCBI nr
Match: XP_038906342.1 (exo-poly-alpha-D-galacturonosidase [Benincasa hispida])

HSP 1 Score: 905.2 bits (2338), Expect = 2.3e-259
Identity = 428/455 (94.07%), Postives = 439/455 (96.48%), Query Frame = 0

Query: 15  FFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDSCPSPARCFV 74
           FFFFLL IHSHCAIPSIRLP RPTSFSV DFGAIGDGLHYDTAAIQSAI+SCPSP+RC+V
Sbjct: 32  FFFFLLIIHSHCAIPSIRLPRRPTSFSVTDFGAIGDGLHYDTAAIQSAINSCPSPSRCYV 91

Query: 75  TFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVVAENTSDVGISGG 134
            FPPGTYLTATIWLRSGVVLDIQPGATVLAGT+MEDYPADSSRWYAVVAEN SDVGISGG
Sbjct: 92  KFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPADSSRWYAVVAENASDVGISGG 151

Query: 135 GAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAH 194
           G V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDL+GFIGS NVRVSNVSFNQPAH
Sbjct: 152 GTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVSNVSFNQPAH 211

Query: 195 WCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTSNGP 254
           WCLHLVRCENTVI+DVSIYGDFDTPNNDGIDIEDSNNT ITRCRIDTGDDAICPKTSNGP
Sbjct: 212 WCLHLVRCENTVIKDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDAICPKTSNGP 271

Query: 255 VFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRDGGSANDITF 314
           VFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQLRDGGSANDITF
Sbjct: 272 VFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRDGGSANDITF 331

Query: 315 SNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITATSENGVFLSGSNS 374
           SNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNI FINITA SENGVFLSGS S
Sbjct: 332 SNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNIRFINITAKSENGVFLSGSKS 391

Query: 375 GVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGLSFENVDMKW 434
           GVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGL+ ENVDM W
Sbjct: 392 GVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGLNLENVDMNW 451

Query: 435 SDDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           SD+DGSLQWNNPLDFRPSTVNNISF NFHSGY  E
Sbjct: 452 SDNDGSLQWNNPLDFRPSTVNNISFSNFHSGYFSE 486

BLAST of Lag0026580 vs. NCBI nr
Match: XP_023538800.1 (uncharacterized protein LOC111799618 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 431/470 (91.70%), Postives = 444/470 (94.47%), Query Frame = 0

Query: 1   MIQHLMAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQ 60
           M QH MAI      FFFF L IHSHC +PSIRLPG  TSFSV DFGAIGDG+HYDTAAIQ
Sbjct: 3   MNQHPMAI-----LFFFFSLIIHSHCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQ 62

Query: 61  SAIDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYA 120
           SAI+SCPSP RC+V FPPGTYLTATIWL+SGVVLDIQPGATVLAGTRMEDYP DSSRWYA
Sbjct: 63  SAINSCPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYA 122

Query: 121 VVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSM 180
           VVAEN SDV ISGGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDLIGFIGS 
Sbjct: 123 VVAENASDVEISGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSK 182

Query: 181 NVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 240
           NVRVSNVS NQPAHWCLHLVRCENT+IEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID
Sbjct: 183 NVRVSNVSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 242

Query: 241 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLG 300
           TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLG
Sbjct: 243 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLG 302

Query: 301 FQLRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINIT 360
           FQLRDGGSANDITFSNMN++TRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNINFINIT
Sbjct: 303 FQLRDGGSANDITFSNMNVSTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINIT 362

Query: 361 ATSENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIME 420
           ATSENGVFLSGS SGVLSNLRF NVKLTYKRWTKYGGG+ADYRPGCQGFVKHGMAGMIME
Sbjct: 363 ATSENGVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIME 422

Query: 421 HIEGLSFENVDMKWS-DDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           HIEGLSFENVDMKWS DDDGSLQWNNPLDFRPSTV+NISFLNFHSGYLRE
Sbjct: 423 HIEGLSFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYLRE 467

BLAST of Lag0026580 vs. NCBI nr
Match: KAG7027765.1 (Exo-poly-alpha-D-galacturonosidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.5 bits (2318), Expect = 4.9e-257
Identity = 429/468 (91.67%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 3   QHLMAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSA 62
           QH MAI      FFFF L IHSHC +PSIRLPG  TSFSV DFGAIGDG+HYDTAAIQSA
Sbjct: 6   QHPMAI-----LFFFFSLIIHSHCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQSA 65

Query: 63  IDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVV 122
           I+SCPSP RC+V FPPGTYLTATIWL+SGVVLDIQPGATVLAGTRMEDYP DSSRWYAVV
Sbjct: 66  INSCPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYAVV 125

Query: 123 AENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNV 182
           AEN SDV I+GGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDLIGFIGS NV
Sbjct: 126 AENASDVEITGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSKNV 185

Query: 183 RVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTG 242
           RVSNVS NQPAHWCLHLVRCENT+IEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTG
Sbjct: 186 RVSNVSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTG 245

Query: 243 DDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQ 302
           DDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQ
Sbjct: 246 DDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQ 305

Query: 303 LRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITAT 362
           LRDGGSANDITFSNMNI+TRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNINFINITAT
Sbjct: 306 LRDGGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINITAT 365

Query: 363 SENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHI 422
           SENGVFLSGS SGVLSNLRF NVKLTYKRWTKYGGG+ADYRPGCQGFVKHGMAGMIMEHI
Sbjct: 366 SENGVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHI 425

Query: 423 EGLSFENVDMKWS-DDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           EGLSFENVDMKWS DDDGSLQWNNPLDFRPSTV+NISF NFHSGYLRE
Sbjct: 426 EGLSFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFFNFHSGYLRE 468

BLAST of Lag0026580 vs. NCBI nr
Match: XP_022971216.1 (uncharacterized protein LOC111470006 [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 428/470 (91.06%), Postives = 441/470 (93.83%), Query Frame = 0

Query: 1   MIQHLMAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQ 60
           M QH MAI L    FFFF L IHS C +PSIRLPG  TSFSV DFGAIGDG+HYDTAAIQ
Sbjct: 3   MNQHPMAILL----FFFFSLIIHSRCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQ 62

Query: 61  SAIDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYA 120
           SAI+SCPSP RC+V FPPGTYLTATIWL+SGVVLDIQPGATVLAGTRMEDYP DSSRWYA
Sbjct: 63  SAINSCPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYA 122

Query: 121 VVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSM 180
           VVAEN SDV I GGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDLIGFIGS 
Sbjct: 123 VVAENASDVEIIGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSK 182

Query: 181 NVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 240
           NVRVSNVS NQPAHWCLHLV CENT+IEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID
Sbjct: 183 NVRVSNVSLNQPAHWCLHLVXCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 242

Query: 241 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLG 300
           TGDDAICPK SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLG
Sbjct: 243 TGDDAICPKASNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLG 302

Query: 301 FQLRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINIT 360
           FQLRDGGSANDITFSNMNI+TRYYDPSWWGRAEPIYVTTCPRD GSKEGSISN+NFINIT
Sbjct: 303 FQLRDGGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNVNFINIT 362

Query: 361 ATSENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIME 420
           ATSENGVFLSGS SGVLSNLRF NVKLTYKRWTKYGGG+ADYRPGCQGFVKHGMAGMIME
Sbjct: 363 ATSENGVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIME 422

Query: 421 HIEGLSFENVDMKWS-DDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           HIEGLSFENVDMKWS DDDGSLQWNNPLDFRPSTV+NISFLNFHSGYLRE
Sbjct: 423 HIEGLSFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYLRE 468

BLAST of Lag0026580 vs. NCBI nr
Match: XP_008443094.1 (PREDICTED: exo-poly-alpha-D-galacturonosidase [Cucumis melo] >TYK25426.1 exo-poly-alpha-D-galacturonosidase [Cucumis melo var. makuwa])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 424/461 (91.97%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 6   MAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDS 65
           M I +  FFFFFFLL IHSH AIPSIRL  R TSFSV DFGAIGDGLHYDTAAIQSAI+S
Sbjct: 3   MDIAIQLFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINS 62

Query: 66  CPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVVAEN 125
           CP+P+RC+VTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT+MEDYP DSSRWYAVVAEN
Sbjct: 63  CPAPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAEN 122

Query: 126 TSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVS 185
            SDVGISGGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDL+GFIGS NVRVS
Sbjct: 123 ASDVGISGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVS 182

Query: 186 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 245
           NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNT ITRCRIDTGDDA
Sbjct: 183 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDA 242

Query: 246 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD 305
           ICPK+SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQLRD
Sbjct: 243 ICPKSSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 302

Query: 306 GGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITATSEN 365
           GGSANDITFSN+NITTRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNI FINITATSEN
Sbjct: 303 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSEN 362

Query: 366 GVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGL 425
           GVFLSGS SGVLSNLRFTNVKL YKRWTKYGGG+ADYRPGCQGFVKHGMAGMIMEHIEGL
Sbjct: 363 GVFLSGSKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 422

Query: 426 SFENVDMKWSDDDGSLQWNNPLDFRPSTVNNISFLNFHSGY 467
           + ENVDM WSD DGSLQWNNPLDFRPSTVNNISF NFHSGY
Sbjct: 423 NLENVDMNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSGY 463

BLAST of Lag0026580 vs. ExPASy Swiss-Prot
Match: P15922 (Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.7e-23
Identity = 91/333 (27.33%), Postives = 149/333 (44.74%), Query Frame = 0

Query: 37  PTSFSVADFGAIGDGLHYDTAAIQSAIDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDI 96
           P   ++  +GA GDG   +T+AIQ AID+CP+   C +  P G + T  +WL+S + L++
Sbjct: 149 PKVINITQYGAKGDGTTLNTSAIQKAIDACPT--GCRIDVPAGVFKTGALWLKSDMTLNL 208

Query: 97  QPGATVLAGTRMEDYPADSSRWYAVVAE------------NTSDVG------ISGGGAVE 156
             GAT+L      DYP D+ + Y+ V++            N+S VG      I G G ++
Sbjct: 209 LQGATLLGSDNAADYP-DAYKIYSYVSQVRPASLLNAIDKNSSAVGTFKNIRIVGKGIID 268

Query: 157 GQGLK---------------FVERFDKR--KNVMVSWNKTGACYG---------DECRPD 216
           G G K               +V+  + +  K+ +++ N+  A             + R  
Sbjct: 269 GNGWKRSADAKDELGNTLPQYVKSDNSKVSKDGILAKNQVAAAVATGMDTKTAYSQRRSS 328

Query: 217 LIGFIGSMNVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNT 276
           L+   G  N  +++V+   PA+  +  +  EN V+E+  I+  F+  N DG++  +S N 
Sbjct: 329 LVTLRGVQNAYIADVTIRNPANHGIMFLESEN-VVENSVIHQTFNANNGDGVEFGNSQNI 388

Query: 277 FITRCRIDTGDDAICPKTSNG-------PVFNLTATNCWIRTKSSAIKLGSASWFNFTQM 317
            +     DTGDD+I      G       P  N    N + R    A+ LGS +      +
Sbjct: 389 MVFNSVFDTGDDSINFAAGMGQDAQKQEPSQNAWLFNNFFRHGHGAVVLGSHTGAGIVDV 448

BLAST of Lag0026580 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 1.6e-16
Identity = 81/292 (27.74%), Postives = 131/292 (44.86%), Query Frame = 0

Query: 37  PTSFSVADFGAIGDGLHYDTAAIQSA-IDSCPSPARCFVTFPPG-TYLTATIWLRS--GV 96
           PT+ SV+DFGA GDG   DT A  +A   +C S     +  P G TYL  +I L      
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNS 124

Query: 97  VLDIQPGATVLAGTRMEDYPADSSRWYAVVAENTSDVGISGG--GAVEGQGLKFVERFDK 156
           +L +Q   T+ A  +  DY  D S+W  ++ +  +++ + GG  G V+G G  + +   K
Sbjct: 125 ILTVQIFGTLSASQKRSDY-KDISKW--IMFDGVNNLSVDGGDTGVVDGNGETWWQNSCK 184

Query: 157 RKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAHWCLHLVRCENTVIEDV 216
           R       NK   C      P  + F  S ++ V N+         + + +C N  + +V
Sbjct: 185 R-------NKAKPC---TKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNV 244

Query: 217 SIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTSNGPVFNLTATNCWIRTKSS 276
            +    D+PN DGI I ++ N  ++   I TGDD I  ++ +  V  +    C      S
Sbjct: 245 VVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNV-QINDITCGPGHGIS 304

Query: 277 AIKLGSASWFNFTQ-MLFDNITIVDSHRGLGFQLRDGGS--ANDITFSNMNI 320
              LG  +   F   +  D   +  +  G+  +   GGS  A++I F N+ +
Sbjct: 305 IGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQM 342

BLAST of Lag0026580 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-14
Identity = 85/342 (24.85%), Postives = 147/342 (42.98%), Query Frame = 0

Query: 39  SFSVADFGAIGDGLHYDTAAIQSA-IDSCPSPARCFVTFPPGTYLTATIWLRSGVVLD-- 98
           SF +   GA G+G    T A+Q A   +C    +  +  P G +L   +        D  
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVT 99

Query: 99  IQPGATVLAGTRMEDYPADSSRWYAVVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVM 158
           IQ    +LA T +  Y  D   W  ++     ++ I+G G ++GQG              
Sbjct: 100 IQVDGNLLATTDLSQY-KDHGNWIEIL--RVDNLVITGKGNLDGQG-------------P 159

Query: 159 VSWNKTGACYGDECR--PDLIGFIGSMNVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIY 218
             W+K       +C+  P+ +      N  VS V+      + +++ RC++ +I+DV++ 
Sbjct: 160 AVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVT 219

Query: 219 GDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAIC--PKTSNGPVFNLTATNCWIRTKSSA 278
              D+PN DGI + DS+   IT   I  GDD I   P TS     N+T   C        
Sbjct: 220 APGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSK---VNITGVTC---GPGHG 279

Query: 279 IKLGSASWF----NFTQMLFDNITIVDSHRGLGFQLRDGGSANDITFSNMNITTRYYDPS 338
           I +GS   +    + T +   + T+  +  G+  +  +  +A+ +T S ++    Y +  
Sbjct: 280 ISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYE-DAASVLTVSKIH----YENIK 339

Query: 339 WWGRAEPIYVTT--CPRD----SGSKEGSISNINFINITATS 364
               A PI++    CP      +G+ + ++ ++ F NIT TS
Sbjct: 340 MEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTS 354

BLAST of Lag0026580 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.5e-14
Identity = 84/342 (24.56%), Postives = 147/342 (42.98%), Query Frame = 0

Query: 39  SFSVADFGAIGDGLHYDTAAIQSA-IDSCPSPARCFVTFPPGTYLTATIWLRSGVVLD-- 98
           SF +   GA G+G    T A+Q A   +C    +  +  P G +L   +        D  
Sbjct: 40  SFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVT 99

Query: 99  IQPGATVLAGTRMEDYPADSSRWYAVVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVM 158
           IQ    +LA T +  Y  +   W  ++     ++ I+G G ++GQG              
Sbjct: 100 IQVDGNLLATTDLSQY-KEHGNWIEIL--RVDNLVITGKGNLDGQG-------------P 159

Query: 159 VSWNKTGACYGDECR--PDLIGFIGSMNVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIY 218
             W+K       +C+  P+ +      N  VS V+      + +++ +C+N +I+DV++ 
Sbjct: 160 AVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVT 219

Query: 219 GDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAIC--PKTSNGPVFNLTATNCWIRTKSSA 278
              D+PN DGI + DS+   IT   I  GDD I   P TS     N+T   C        
Sbjct: 220 APGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSK---VNITGVTC---GPGHG 279

Query: 279 IKLGSASWF----NFTQMLFDNITIVDSHRGLGFQLRDGGSANDITFSNMNITTRYYDPS 338
           I +GS   +    + T +   + T+  +  G+  +  +  +A+ +T S ++    Y +  
Sbjct: 280 ISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYE-DAASVLTVSKIH----YENIK 339

Query: 339 WWGRAEPIYVTT--CPRD----SGSKEGSISNINFINITATS 364
               A PI++    CP      +G+ + ++ ++ F NIT TS
Sbjct: 340 MEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTS 354

BLAST of Lag0026580 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 9.6e-14
Identity = 77/290 (26.55%), Postives = 117/290 (40.34%), Query Frame = 0

Query: 40  FSVADFGAIGDGLHYDTAAIQSAIDSCPSPARCFVTFPPGTYLTATIWLRSGVV---LDI 99
           F V  FGA+GDG   DTAA Q A  +  +     V  P G     T  + SG     L  
Sbjct: 80  FDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKPGLVF 139

Query: 100 QPGATVLAGTRMEDYPADSSRWYAVVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVMV 159
           Q    ++     E++P   ++   +V         SG G VEG G K+ +   K      
Sbjct: 140 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 199

Query: 160 SWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDF 219
             + +G C      P +I F  S N+ V  +       + +    C+  +I ++ I    
Sbjct: 200 GSSSSGPC----ASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPK 259

Query: 220 DTPNNDGIDIEDSNNTFITRCRIDTGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGS 279
            +PN DGI + ++ +  I    +  GDD I   T    V ++    C     S  I +GS
Sbjct: 260 LSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDV-DIQGVTC---GPSHGISIGS 319

Query: 280 ASWFNFTQMLFDNIT-----IVDSHRGLGFQLRDG--GSANDITFSNMNI 320
               N +Q    NIT     I DS  GL  +   G  GS +++ F N+ +
Sbjct: 320 LGVHN-SQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQM 360

BLAST of Lag0026580 vs. ExPASy TrEMBL
Match: A0A6J1I2Q7 (uncharacterized protein LOC111470006 OS=Cucurbita maxima OX=3661 GN=LOC111470006 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 428/470 (91.06%), Postives = 441/470 (93.83%), Query Frame = 0

Query: 1   MIQHLMAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQ 60
           M QH MAI L    FFFF L IHS C +PSIRLPG  TSFSV DFGAIGDG+HYDTAAIQ
Sbjct: 3   MNQHPMAILL----FFFFSLIIHSRCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQ 62

Query: 61  SAIDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYA 120
           SAI+SCPSP RC+V FPPGTYLTATIWL+SGVVLDIQPGATVLAGTRMEDYP DSSRWYA
Sbjct: 63  SAINSCPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYA 122

Query: 121 VVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSM 180
           VVAEN SDV I GGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDLIGFIGS 
Sbjct: 123 VVAENASDVEIIGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSK 182

Query: 181 NVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 240
           NVRVSNVS NQPAHWCLHLV CENT+IEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID
Sbjct: 183 NVRVSNVSLNQPAHWCLHLVXCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 242

Query: 241 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLG 300
           TGDDAICPK SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLG
Sbjct: 243 TGDDAICPKASNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLG 302

Query: 301 FQLRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINIT 360
           FQLRDGGSANDITFSNMNI+TRYYDPSWWGRAEPIYVTTCPRD GSKEGSISN+NFINIT
Sbjct: 303 FQLRDGGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNVNFINIT 362

Query: 361 ATSENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIME 420
           ATSENGVFLSGS SGVLSNLRF NVKLTYKRWTKYGGG+ADYRPGCQGFVKHGMAGMIME
Sbjct: 363 ATSENGVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIME 422

Query: 421 HIEGLSFENVDMKWS-DDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           HIEGLSFENVDMKWS DDDGSLQWNNPLDFRPSTV+NISFLNFHSGYLRE
Sbjct: 423 HIEGLSFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFLNFHSGYLRE 468

BLAST of Lag0026580 vs. ExPASy TrEMBL
Match: A0A5D3DP49 (Exo-poly-alpha-D-galacturonosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005690 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 424/461 (91.97%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 6   MAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDS 65
           M I +  FFFFFFLL IHSH AIPSIRL  R TSFSV DFGAIGDGLHYDTAAIQSAI+S
Sbjct: 3   MDIAIQLFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINS 62

Query: 66  CPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVVAEN 125
           CP+P+RC+VTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT+MEDYP DSSRWYAVVAEN
Sbjct: 63  CPAPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAEN 122

Query: 126 TSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVS 185
            SDVGISGGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDL+GFIGS NVRVS
Sbjct: 123 ASDVGISGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVS 182

Query: 186 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 245
           NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNT ITRCRIDTGDDA
Sbjct: 183 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDA 242

Query: 246 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD 305
           ICPK+SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQLRD
Sbjct: 243 ICPKSSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 302

Query: 306 GGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITATSEN 365
           GGSANDITFSN+NITTRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNI FINITATSEN
Sbjct: 303 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSEN 362

Query: 366 GVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGL 425
           GVFLSGS SGVLSNLRFTNVKL YKRWTKYGGG+ADYRPGCQGFVKHGMAGMIMEHIEGL
Sbjct: 363 GVFLSGSKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 422

Query: 426 SFENVDMKWSDDDGSLQWNNPLDFRPSTVNNISFLNFHSGY 467
           + ENVDM WSD DGSLQWNNPLDFRPSTVNNISF NFHSGY
Sbjct: 423 NLENVDMNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSGY 463

BLAST of Lag0026580 vs. ExPASy TrEMBL
Match: A0A1S3B7Z6 (exo-poly-alpha-D-galacturonosidase OS=Cucumis melo OX=3656 GN=LOC103486792 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 424/461 (91.97%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 6   MAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDS 65
           M I +  FFFFFFLL IHSH AIPSIRL  R TSFSV DFGAIGDGLHYDTAAIQSAI+S
Sbjct: 3   MDIAIQLFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINS 62

Query: 66  CPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVVAEN 125
           CP+P+RC+VTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT+MEDYP DSSRWYAVVAEN
Sbjct: 63  CPAPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAEN 122

Query: 126 TSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVS 185
            SDVGISGGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDL+GFIGS NVRVS
Sbjct: 123 ASDVGISGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVS 182

Query: 186 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 245
           NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNT ITRCRIDTGDDA
Sbjct: 183 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDA 242

Query: 246 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD 305
           ICPK+SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQLRD
Sbjct: 243 ICPKSSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 302

Query: 306 GGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITATSEN 365
           GGSANDITFSN+NITTRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNI FINITATSEN
Sbjct: 303 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSEN 362

Query: 366 GVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGL 425
           GVFLSGS SGVLSNLRFTNVKL YKRWTKYGGG+ADYRPGCQGFVKHGMAGMIMEHIEGL
Sbjct: 363 GVFLSGSKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 422

Query: 426 SFENVDMKWSDDDGSLQWNNPLDFRPSTVNNISFLNFHSGY 467
           + ENVDM WSD DGSLQWNNPLDFRPSTVNNISF NFHSGY
Sbjct: 423 NLENVDMNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSGY 463

BLAST of Lag0026580 vs. ExPASy TrEMBL
Match: A0A6J1EUK0 (uncharacterized protein LOC111437911 OS=Cucurbita moschata OX=3662 GN=LOC111437911 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.1e-254
Identity = 427/470 (90.85%), Postives = 441/470 (93.83%), Query Frame = 0

Query: 1   MIQHLMAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQ 60
           M Q  MAI      FFFF L I SHC +PSIRLPG  TSFSV DFGAIGDG+HYDTAAIQ
Sbjct: 3   MNQRPMAI-----LFFFFSLIILSHCVVPSIRLPGGSTSFSVTDFGAIGDGVHYDTAAIQ 62

Query: 61  SAIDSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYA 120
           SAI++CPSP RC+V FPPGTYLTATIWL+SGVVLDIQPGATVLAGTRMEDYP DSSRWYA
Sbjct: 63  SAINNCPSPVRCYVIFPPGTYLTATIWLQSGVVLDIQPGATVLAGTRMEDYPMDSSRWYA 122

Query: 121 VVAENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSM 180
           VVAEN SDV I+GGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDLIGFIGS 
Sbjct: 123 VVAENASDVEITGGGVVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSK 182

Query: 181 NVRVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 240
           NVRVSNVS NQPAHWCLHLVRCENT+IEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID
Sbjct: 183 NVRVSNVSLNQPAHWCLHLVRCENTIIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRID 242

Query: 241 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLG 300
           TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLG
Sbjct: 243 TGDDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLG 302

Query: 301 FQLRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINIT 360
           FQLRDGGSANDITFSNMNI+TRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNINFINIT
Sbjct: 303 FQLRDGGSANDITFSNMNISTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNINFINIT 362

Query: 361 ATSENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIME 420
           ATSENGVFLSGS SGVLSNLRF NVKLTYKRWTKYGGG+ADYRPGCQGFVKHGMAGMIME
Sbjct: 363 ATSENGVFLSGSKSGVLSNLRFNNVKLTYKRWTKYGGGIADYRPGCQGFVKHGMAGMIME 422

Query: 421 HIEGLSFENVDMKWS-DDDGSLQWNNPLDFRPSTVNNISFLNFHSGYLRE 470
           HIEGLSFENVDMKWS DDDGSLQWNNPLDFRPSTV+NISF NFHSGYLRE
Sbjct: 423 HIEGLSFENVDMKWSDDDDGSLQWNNPLDFRPSTVSNISFFNFHSGYLRE 467

BLAST of Lag0026580 vs. ExPASy TrEMBL
Match: A0A5A7TLI5 (Exo-poly-alpha-D-galacturonosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G00350 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.0e-253
Identity = 423/461 (91.76%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 6   MAIQLLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDS 65
           M I +  FFFFFFLL IHSH AIPSIRL  R TSFSV DFGAIGDGLHYDTAAIQSAI+S
Sbjct: 3   MDIAIQLFFFFFFLLIIHSHSAIPSIRLLRRSTSFSVTDFGAIGDGLHYDTAAIQSAINS 62

Query: 66  CPSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMEDYPADSSRWYAVVAEN 125
           CP+P+RC+VTFPPGTYLTATIWLRSGVVLDIQPGATVLAGT+MEDYP DSSRWYAVVAEN
Sbjct: 63  CPAPSRCYVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTKMEDYPGDSSRWYAVVAEN 122

Query: 126 TSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVS 185
            SDVGISGGG V+GQGLKFVE+FDKRKNVMVSWNKTGACYGDECRPDL+GFIGS NVRVS
Sbjct: 123 ASDVGISGGGTVDGQGLKFVEKFDKRKNVMVSWNKTGACYGDECRPDLVGFIGSNNVRVS 182

Query: 186 NVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDA 245
           NVSFNQPAH CLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNT ITRCRIDTGDDA
Sbjct: 183 NVSFNQPAH-CLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTLITRCRIDTGDDA 242

Query: 246 ICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD 305
           ICPK+SNGPVFNLTATNCWIRTKSSAIKLGSASWFNFT+MLFDNITIVDSHRGLGFQLRD
Sbjct: 243 ICPKSSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTRMLFDNITIVDSHRGLGFQLRD 302

Query: 306 GGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITATSEN 365
           GGSANDITFSN+NITTRYYDPSWWGRAEPIYVTTCPRD GSKEGSISNI FINITATSEN
Sbjct: 303 GGSANDITFSNINITTRYYDPSWWGRAEPIYVTTCPRDPGSKEGSISNIRFINITATSEN 362

Query: 366 GVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKHGMAGMIMEHIEGL 425
           GVFLSGS SGVLSNLRFTNVKL YKRWTKYGGG+ADYRPGCQGFVKHGMAGMIMEHIEGL
Sbjct: 363 GVFLSGSKSGVLSNLRFTNVKLRYKRWTKYGGGIADYRPGCQGFVKHGMAGMIMEHIEGL 422

Query: 426 SFENVDMKWSDDDGSLQWNNPLDFRPSTVNNISFLNFHSGY 467
           + ENVDM WSD DGSLQWNNPLDFRPSTVNNISF NFHSGY
Sbjct: 423 NLENVDMNWSDTDGSLQWNNPLDFRPSTVNNISFFNFHSGY 462

BLAST of Lag0026580 vs. TAIR 10
Match: AT3G57790.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 641.7 bits (1654), Expect = 4.5e-184
Identity = 302/465 (64.95%), Postives = 370/465 (79.57%), Query Frame = 0

Query: 10  LLFFFFFFFLLTIHSHCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAIDSC--- 69
           +L    FF L+   S  +   I+LPG   + SV DFGA GDG++YDT+AIQS ID+C   
Sbjct: 5   ILLLLLFFSLVQSRSDTSYSKIQLPGDSLTLSVTDFGATGDGINYDTSAIQSTIDACNRH 64

Query: 70  --PSPARCFVTFPPGTYLTATIWLRSGVVLDIQPGATVLAGTRMED-YPAD-SSRWYAVV 129
                + C V FP G YLTA + LRSGV+LD+   A +L G R+ED YPA+ SS WY VV
Sbjct: 65  YTSFSSICRVVFPSGNYLTAKLHLRSGVILDVTENAVLLGGPRIEDYYPAETSSDWYVVV 124

Query: 130 AENTSDVGISGGGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNV 189
           A N +DVGI+GGGA++GQG KFV RFD++KNVMVSWN+TGAC GDECRP L+GF+ S+NV
Sbjct: 125 ANNATDVGITGGGAIDGQGSKFVVRFDEKKNVMVSWNQTGACLGDECRPRLVGFVDSINV 184

Query: 190 RVSNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTG 249
            + N++  +PA+WCLH+VRCENT + DVSI GDF+TPNNDGIDIEDSNNT ITRC IDTG
Sbjct: 185 EIWNITLREPAYWCLHIVRCENTSVHDVSILGDFNTPNNDGIDIEDSNNTVITRCHIDTG 244

Query: 250 DDAICPKTSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQ 309
           DDAICPKT  GP++NLTAT+CWIRTKSSAIKLGSASWF+F  ++FDNITI +SHRGLG Q
Sbjct: 245 DDAICPKTYTGPLYNLTATDCWIRTKSSAIKLGSASWFDFKGLVFDNITIFESHRGLGMQ 304

Query: 310 LRDGGSANDITFSNMNITTRYYDPSWWGRAEPIYVTTCPRDSGSKEGSISNINFINITAT 369
           +RDGG+ +D+TFSN+NI+TRYYDPSWWGRAEPIY+TTCPRDS +KEGSISN+ F+NIT  
Sbjct: 305 IRDGGNVSDVTFSNINISTRYYDPSWWGRAEPIYITTCPRDSSAKEGSISNLLFVNITID 364

Query: 370 SENGVFLSGSNSGVLSNLRFTNVKLTYKRWTKYGGGLADYRPGCQGFVKH-GMAGMIMEH 429
           SENGVFLSGS +G+LS+++F N+ LT++RW+ Y  GL DYRPGCQG V H   +G+IMEH
Sbjct: 365 SENGVFLSGSPNGLLSDIKFKNMNLTFRRWSNYSAGLVDYRPGCQGLVNHRATSGIIMEH 424

Query: 430 IEGLSFENVDMKWSDDDG-SLQWNNPLDFRPSTVNNISFLNFHSG 466
           + G   ENVD+KWSDDD  +  WN PL+FRPSTVNN+SF+ F SG
Sbjct: 425 VNGFRVENVDLKWSDDDDVNAAWNVPLEFRPSTVNNVSFVGFTSG 469

BLAST of Lag0026580 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 5.0e-26
Identity = 111/422 (26.30%), Postives = 178/422 (42.18%), Query Frame = 0

Query: 25  HCAIPSIRLPGRPTSFSVADFGAIGDGLHYDTAAIQSAI-----DSCPSPARCFVTFPPG 84
           HC   ++    RP S S+ +FGA+GDG+  +T A Q+A+      S    A+ FV  P G
Sbjct: 28  HCEYSNLASLHRPHSVSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFV--PAG 87

Query: 85  TYLTATIWLRSGVVLDIQPGATVLAGTRMEDYP-ADSSRWYA-------------VVAEN 144
            +LT +  L S + L +  GAT+L  T  E++P  D    Y              +  +N
Sbjct: 88  QWLTGSFDLISHLTLWLDKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQN 147

Query: 145 TSDVGISG-GGAVEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRV 204
            +DV I+G  G ++GQG            V   W + G    +  RP L+  + S  + +
Sbjct: 148 LTDVVITGENGTIDGQG-----------TVWWDWFRNGEL--NYTRPHLVELMNSTGLII 207

Query: 205 SNVSFNQPAHWCLHLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDD 264
           SN++F     W +H V C + V+++++I    ++PN DG+D + S N  I  C I TGDD
Sbjct: 208 SNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSSTNVCIEDCYIVTGDD 267

Query: 265 AICPK-------------TSNGPVFNLTATNCWIRTKSSAIKLGSASWFNFTQMLFDNIT 324
            +  K             +S   +  LT       T SS I +GS      +++   ++ 
Sbjct: 268 LVSIKSGWDEYGISYARPSSKIKINRLTGQT----TSSSGIAIGSEMSGGVSEIYIKDLH 327

Query: 325 IVDSHRGLGFQLR--DGGSANDITFSNMNI---------TTRY-------YDPSWWGRAE 384
           + +S+ G+  +     GG   ++   N+ +         T +Y       YDP    +A 
Sbjct: 328 LFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDP----KAL 387

Query: 385 PIYVTTCPRDSGSKEGSISNINFINITATSEN-GVFLSGSNSGVLSNLRFTNVKLTYKRW 395
           P               +I  I F N+          L G    V  N+ F NV L  K+ 
Sbjct: 388 P---------------AIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKN 411

BLAST of Lag0026580 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 2.3e-23
Identity = 94/319 (29.47%), Postives = 141/319 (44.20%), Query Frame = 0

Query: 36  RPTSFSVADFGAIGDGLHYDTAAIQSAIDSCPSPAR---CFVTFPPGTYLTATIWLRSGV 95
           R    S+ DFG +GDG   +T A ++AI       R     +  PPG YLT +  L S +
Sbjct: 37  RYDKISITDFGGVGDGRTVNTKAFRAAIYRIQHLKRRGGTLLYIPPGVYLTESFNLTSHM 96

Query: 96  VLDIQPGATVLAGTRMEDYP-------------ADSSRWYAVV-AENTSDVGISG-GGAV 155
            L +  GA + A     ++P                 R+ + +  +   DV I+G  G +
Sbjct: 97  TLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGRELPGGRYMSFIHGDGLRDVVITGQNGTI 156

Query: 156 EGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAHWCL 215
           +GQG              V WN   +      RP+LI F  S  + +SNV F     W +
Sbjct: 157 DGQG-------------EVWWNMWRSRTLKYTRPNLIEFKDSKEIIISNVIFQNSPFWNI 216

Query: 216 HLVRCENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAICPKT---SNGP 275
           H V C N VI  V+I    D+PN DGID + S N  I    I TGDD +  K+     G 
Sbjct: 217 HPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDDLVAIKSGWDQYGI 276

Query: 276 VFNLTATNCWIR--TKSS---AIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD---- 325
            +   ++N  IR  T SS    I +GS +      ++ ++IT+  S+ G+G  ++     
Sbjct: 277 AYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHITL--SNMGVGVNIKTNIGR 336

BLAST of Lag0026580 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 3.1e-23
Identity = 84/306 (27.45%), Postives = 141/306 (46.08%), Query Frame = 0

Query: 41  SVADFGAIGDGLHYDTAAIQSAI---DSCPSPARCFVTFPPGTYLTATIWLRSGVVLDIQ 100
           S+ DFG +GDG   +TAA + A+   +   +     +  P GT+L+ +  L S   L ++
Sbjct: 73  SITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFLE 132

Query: 101 PGATVLAGTRMEDYP-------------ADSSRWYAVV-AENTSDVGISG-GGAVEGQGL 160
            GA +L    ++++P                 R  +++  +N ++V I+G  G ++GQG 
Sbjct: 133 RGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQGK 192

Query: 161 KFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAHWCLHLVRC 220
            + E +         WN+T        R  LI    S N+ +SN++      W +H V C
Sbjct: 193 MWWELW---------WNRTLV----HTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYC 252

Query: 221 ENTVIEDVSIYGDFDTPNNDGIDIEDSNNTFITRCRIDTGDDAICPKT---SNGPVFNLT 280
            N VI +++I    + PN DGID + S N  I  C I++GDD +  K+     G      
Sbjct: 253 SNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARP 312

Query: 281 ATNCWIRTKS------SAIKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD--GGSAND 318
           ++N  IR  S      S + +GS        +  ++I + DS  GL  +     GG  ++
Sbjct: 313 SSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISN 365

BLAST of Lag0026580 vs. TAIR 10
Match: AT3G42950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-22
Identity = 91/315 (28.89%), Postives = 136/315 (43.17%), Query Frame = 0

Query: 36  RPTSFSVADFGAIGDGLHYDTAAIQSAIDSCPSPAR---CFVTFPPGTYLTATIWLRSGV 95
           RP  F++ DFGA+GDG+  +T A + AI      A+     +  PPG +LTA   L S +
Sbjct: 62  RPVVFNLTDFGAVGDGVTINTEAFEKAIYKISKLAKKGGGQLNVPPGRWLTAPFNLTSYM 121

Query: 96  VLDIQPGATVLAGTRMEDY----PADSSRWYA-----------VVAENTSDVGISG-GGA 155
            L +   A +LA  + E Y    P   S  Y            +  +N  DV ++G  G+
Sbjct: 122 TLFLSENAEILA-LQDEKYWSLLPPLPSYGYGREHHGPRYGSFIHGQNLRDVVVTGNNGS 181

Query: 156 VEGQGLKFVERFDKRKNVMVSWNKTGACYGDECRPDLIGFIGSMNVRVSNVSFNQPAHWC 215
           + GQG  +             W K      +  R  L+  + S ++  +N++      W 
Sbjct: 182 INGQGQTW-------------WKKYRQKLLNHTRGPLVQIMWSSDIVFANITLRDSPFWT 241

Query: 216 LHLVRCENTVIEDVSIYGD-FDTPNNDGIDIEDSNNTFITRCRIDTGDDAICPK------ 275
           LH   C+N  I +++I    F+ PN DGID +   +  I    I  GDD I  K      
Sbjct: 242 LHPYDCKNVTITNMTILAPVFEAPNTDGIDPDSCEDMLIENSYISVGDDGIAIKSGWDQY 301

Query: 276 --TSNGPVFNLTATNCWIRTKSSA-IKLGSASWFNFTQMLFDNITIVDSHRGLGFQLRD- 320
             T   P  N+   N  IR+  SA I +GS      + +  +NI I  S RG+  +    
Sbjct: 302 GTTYGKPSKNILIRNLIIRSMVSAGISIGSEMSGGVSNITVENILIWSSRRGVRIKTAPG 361