Lag0026523 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026523
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr10: 38414433 .. 38417284 (-)
RNA-Seq Expression	Lag0026523
Synteny	Lag0026523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCGAAGGTGGCAGTGCCAGGATGCGAGCGCGACATTGTTTGTGTGCAAGGATAGATCGTCTGGACGCTAGGGCAGCGGTGACAACGTCATTTGAGTGCTAGGGCAACGACGACGTTGGGATGTGGGTTGTGTTGGGTACAGTGTCGACGTCAGATTAGGTTAGATAGAGGAGCCAAGATGATTTCGGAGAGGATGGGCGACGACAACTAGGGTTCCGACAACGATAGCGGCTAGCGTTCCAGTGAATGACGACTAGAGTTTGAATAGTTTCAAAGCTCTGATACCATGAAGCTATGTATAAAATATAATATTTCTTCTTGTTATTCATTGAGTTAGGAAGAAGAACTTTATACAAAAAGACCCTCAATCAAATATGCAAAAAGATCTGCTAACAATCTAAACCTGTATGGAAAAGAAAAATACAACTATAAACATATACAAGGAAATAGCTATCAACACACCTAAGGAAACAATCTAAAAAATAAAGCCCTAATTTACAGCTTTATTTTCTAGAGAGGGCCAAGGTTCTTTGGGATTGTGCGGTTAAACCAATGCTTTGTTCAACTTGGAAGGAAAGGAATTTTAGAATATTTGAAGATGGGTGAAGCTCAGAAGATTCTTTTGTGTATATACAACACTTGGCTTCTTGGTGGAGTTCAAGTCACAAAGATTATTAGTTTATTTATAGGCTTGCCTTGATTATTAGAAATTGGAAGGCTTTTTTGTAATTGTTTTTGTAATAGTTTCTTTGTTTGGGGAGGGTTCCCTCAACCTCTTGCCCTTAGGTTGTCTTCCTTTTGATGATGAATATATTTCTCTCTTTCTTATTAAAGAAAAAGAATTAACGGGTGTATAAGAATTTCAACAATACAGCGGATTGTGCAAGAACAACAAGAACATCAGATATGGGGAGCCTATGCACAAAGGTTGCTAGATCCTGGATCAGGACTTTGGAGGAATCCTAGTGGTGGCGGCCATGATGACAAAGCCCATCCACCTATTCACCCAACAAAGTTCTCAGCCGGGGAACGTGGATGGAGCCAAGATCACCATGTAATAGTTTTTCTTTACTTGTCTGTTAAATTGCATATTTGTGTTAAAAGTTTTTTTCCTCTTTTCATTTTGGTCTTTGTAAATTGTCATCTTGTAGTCCTCAGTCATCAATAATAGTGAGATTTTTTCTTCCCAGAGATTATATGAGCTAGTCGTTCGCCATTTCCTTGCATGTGTCTCACAGCCAGCTGTAGGTGCTGTGACTACTGTTGAAATTGATATTGCTGGTGAATTGTTTTCTACATCTGGGAGAATGATACTAGCAGTGAGTTCTCTACACAAATTTTCTGAATCTTTTATTTTTAAAATGGCCAATAGTATTTTGAGGTAATGCCTTGGTGATTGGCAAGATGGGACGAGCTGCATGTACCCACCTTGTTGAATGCCTCCACTTGTGTCTTACAAAACAGGCAAAATTGTTAGGTTTCGTAGCACATTGGACTACACTATATTCTTTTCATTCATCATTTTTTGAAAGTTAAGAGGTCGTGATCAAAGTAATCTATTAAATAAAAACGTAATTATGGTTTTGTTTCTTGTAGATTATTTCATTCTATTTTATTTTTCCCTTATTTAGATTAGATTTTATTCTATCTTAATGTCTTTCCTTTTCTGTATTTTTCCTCAACTCTATATCTTATTGTTTTTTGGAGATTAGTAAATGAGACATTATTTTTCTTCCAAAAACTACATGGTATCAGAGCAAACCCTAAAATAAAACCCTAGCCGTCAAAACCCTAATTCCTCCGGCGAACTCCTCTTCCGACCACACTCCGACGAACCCTCCTCCCGACGACCCATTCACCGGCGAGCTCTCTCACCGGTGTTTCGGATTCTTGCACGTATTCTCCGGTGTTCGCGTCTGTTTGTCTGCCTTCGTTCGGCCGTCTGTGGGTTTCGTTGGTTCGTGGTTCTTTCTGGTTTCGTCCTTTCGTCGTCTTCTGTGGGTTTCTTGATCTCTTTCAGCATCAGTTCATCGTGGTTTTTCAGTTTTCGTTCAGCGTTCGGCCGTCTCTTGGTTTTCATCCACTGTGGGTTTCGTTCCTTAGTTCGTGATAGTGGTTTATTTGTGCTACTCGTCACCATCGCCGCTGGTTTTTTTTGTGTGTTATCTGTTGATTTTCGTTGGAGCAGTCAACTTTGTGTGTTTCTTCAAATTTTTCAGAGGTTGCAAATGGCAAAACATGGATTGGCAAGTGTTAGTTATGAAAACTCTCCTCAACCTCAAGATTCCTCTCATCAAATTGTGACTAATCATAAACTTCAAGGTCACAATTTTCTTCAATGGAATCAATCAGTTTTCATATATATATGTGGTCGCGGCAAAGATGGTCATCTAACTGGCGATACTCCTGCACCAGATGTTAAAGACCCAAAATTTCGATCATGGAAAACGGATGATCATTTAGTCATGTCTTGGCTACTAAATTCTATGACTCCAGAGGTTGGAGAGAATTTTCTATTGTTCAAAACGACAAAAGAGATATGGGATGCTGCCCGTGATACTTACTCTAGCTTTGAGAATTCTTCTGCACTTCTGGCTATTGAAACTCGGTTGTATGACCTACGTCAAGGGGACCTTAATGTCACTCAATATTTTAATCTTCTTGTTCGCAATTGGCAACATTTAGATATGTACGAAACTTACGAATGGAAGTGTGGAGAAGACATGGCCCTTTACAAGAAGATAGTTGAGAAAAAGAGAATACTGAGGTTCCTTCTAAGACTTAATAAAGAGCTAGACGACGTAAGAGGAAGAATAATGGGAGCAAAAGATCTACCCTCTCTTAGATAA

mRNA sequence

ATGTGCGAAGGTGGCAGTGCCAGGATGCGAGCGCGACATTGTTTGTGTGCAAGGATAGATCGTCTGGACGCTAGGGCAGCGCGGATTGTGCAAGAACAACAAGAACATCAGATATGGGGAGCCTATGCACAAAGGTTGCTAGATCCTGGATCAGGACTTTGGAGGAATCCTAGTGGTGGCGGCCATGATGACAAAGCCCATCCACCTATTCACCCAACAAAGTTCTCAGCCGGGGAACGTGGATGGAGCCAAGATCACCATTCCTCAGTCATCAATAATAGTGAGATTTTTTCTTCCCAGAGATTATATGAGCTAGTCGTTCGCCATTTCCTTGCATGTGTCTCACAGCCAGCTGTAGGTGCTGTGACTACTGTTGAAATTGATATTGCTGGTGAATTGTTTTCTACATCTGGGAGAATGATACTAGCAGTGAGTTCTCTACACAAATTTTCTGAATCTTTTATTTTTAAAATGGCCAATAGTATTTTGAGCCGTCAAAACCCTAATTCCTCCGGCGAACTCCTCTTCCGACCACACTCCGACGAACCCTCCTCCCGACGACCCATTCACCGGCGAGCTCTCTCACCGGTGTTTCGGATTCTTGCACGTATTCTCCGGTGTTCGCGTCTGTTTGTCTGCCTTCGTTCGGCCGTCTGTGGGTTTCGTTGGTTCGTGGTTCTTTCTGGTTTCGTCCTTTCGTCGTCTTCTGTGGGTTTCTTGATCTCTTTCAGCATCAGTTCATCGTGGTTTTTCAGTTTTCGTTCAGCGTTCGGCCGTCTCTTGGTTTTCATCCACTGTGGGTTTCGTTCCTTAGTTCGTGATAGTGGTTTATTTGTGCTACTCGTCACCATCGCCGCTGGTTTTTTTTGTGTGTTATCTGTTGATTTTCGTTGGAGCAGTCAACTTTGTGTGTTTCTTCAAATTTTTCAGAGGTTGCAAATGGCAAAACATGGATTGGCAAGTGTTAGTTATGAAAACTCTCCTCAACCTCAAGATTCCTCTCATCAAATTGTGACTAATCATAAACTTCAAGGTCACAATTTTCTTCAATGGAATCAATCAGTTTTCATATATATATGTGGTCGCGGCAAAGATGGTCATCTAACTGGCGATACTCCTGCACCAGATGTTAAAGACCCAAAATTTCGATCATGGAAAACGGATGATCATTTAGTCATGTCTTGGCTACTAAATTCTATGACTCCAGAGGTTGGAGAGAATTTTCTATTGTTCAAAACGACAAAAGAGATATGGGATGCTGCCCGTGATACTTACTCTAGCTTTGAGAATTCTTCTGCACTTCTGGCTATTGAAACTCGGTTGTATGACCTACGTCAAGGGGACCTTAATGTCACTCAATATTTTAATCTTCTTGTTCGCAATTGGCAACATTTAGATATGTACGAAACTTACGAATGGAAGTGTGGAGAAGACATGGCCCTTTACAAGAAGATAGTTGAGAAAAAGAGAATACTGAGGTTCCTTCTAAGACTTAATAAAGAGCTAGACGACGTAAGAGGAAGAATAATGGGAGCAAAAGATCTACCCTCTCTTAGATAA

Coding sequence (CDS)

ATGTGCGAAGGTGGCAGTGCCAGGATGCGAGCGCGACATTGTTTGTGTGCAAGGATAGATCGTCTGGACGCTAGGGCAGCGCGGATTGTGCAAGAACAACAAGAACATCAGATATGGGGAGCCTATGCACAAAGGTTGCTAGATCCTGGATCAGGACTTTGGAGGAATCCTAGTGGTGGCGGCCATGATGACAAAGCCCATCCACCTATTCACCCAACAAAGTTCTCAGCCGGGGAACGTGGATGGAGCCAAGATCACCATTCCTCAGTCATCAATAATAGTGAGATTTTTTCTTCCCAGAGATTATATGAGCTAGTCGTTCGCCATTTCCTTGCATGTGTCTCACAGCCAGCTGTAGGTGCTGTGACTACTGTTGAAATTGATATTGCTGGTGAATTGTTTTCTACATCTGGGAGAATGATACTAGCAGTGAGTTCTCTACACAAATTTTCTGAATCTTTTATTTTTAAAATGGCCAATAGTATTTTGAGCCGTCAAAACCCTAATTCCTCCGGCGAACTCCTCTTCCGACCACACTCCGACGAACCCTCCTCCCGACGACCCATTCACCGGCGAGCTCTCTCACCGGTGTTTCGGATTCTTGCACGTATTCTCCGGTGTTCGCGTCTGTTTGTCTGCCTTCGTTCGGCCGTCTGTGGGTTTCGTTGGTTCGTGGTTCTTTCTGGTTTCGTCCTTTCGTCGTCTTCTGTGGGTTTCTTGATCTCTTTCAGCATCAGTTCATCGTGGTTTTTCAGTTTTCGTTCAGCGTTCGGCCGTCTCTTGGTTTTCATCCACTGTGGGTTTCGTTCCTTAGTTCGTGATAGTGGTTTATTTGTGCTACTCGTCACCATCGCCGCTGGTTTTTTTTGTGTGTTATCTGTTGATTTTCGTTGGAGCAGTCAACTTTGTGTGTTTCTTCAAATTTTTCAGAGGTTGCAAATGGCAAAACATGGATTGGCAAGTGTTAGTTATGAAAACTCTCCTCAACCTCAAGATTCCTCTCATCAAATTGTGACTAATCATAAACTTCAAGGTCACAATTTTCTTCAATGGAATCAATCAGTTTTCATATATATATGTGGTCGCGGCAAAGATGGTCATCTAACTGGCGATACTCCTGCACCAGATGTTAAAGACCCAAAATTTCGATCATGGAAAACGGATGATCATTTAGTCATGTCTTGGCTACTAAATTCTATGACTCCAGAGGTTGGAGAGAATTTTCTATTGTTCAAAACGACAAAAGAGATATGGGATGCTGCCCGTGATACTTACTCTAGCTTTGAGAATTCTTCTGCACTTCTGGCTATTGAAACTCGGTTGTATGACCTACGTCAAGGGGACCTTAATGTCACTCAATATTTTAATCTTCTTGTTCGCAATTGGCAACATTTAGATATGTACGAAACTTACGAATGGAAGTGTGGAGAAGACATGGCCCTTTACAAGAAGATAGTTGAGAAAAAGAGAATACTGAGGTTCCTTCTAAGACTTAATAAAGAGCTAGACGACGTAAGAGGAAGAATAATGGGAGCAAAAGATCTACCCTCTCTTAGATAA

Protein sequence

MCEGGSARMRARHCLCARIDRLDARAARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILAVSSLHKFSESFIFKMANSILSRQNPNSSGELLFRPHSDEPSSRRPIHRRALSPVFRILARILRCSRLFVCLRSAVCGFRWFVVLSGFVLSSSSVGFLISFSISSSWFFSFRSAFGRLLVFIHCGFRSLVRDSGLFVLLVTIAAGFFCVLSVDFRWSSQLCVFLQIFQRLQMAKHGLASVSYENSPQPQDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMALYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR

Homology

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BLAST of Lag0026523 vs. NCBI nr
Match: PKA63925.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Apostasia shenzhenica])

HSP 1 Score: 305.1 bits (780), Expect = 1.2e-78
Identity = 145/209 (69.38%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 313 QMAKHGLASVSYENSPQP--QDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTG 372
           Q +   + S S  NS  P   D+S  +VTNHKL GHNFLQW+QSVF+YICGRGKDGHLTG
Sbjct: 4   QSSSSEVTSRSESNSSLPIVSDNSSHLVTNHKLHGHNFLQWSQSVFMYICGRGKDGHLTG 63

Query: 373 DTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFEN 432
           +  AP+  DPK+R+W+TDDHLVMSWL+NSMT EVGENFLLF+T KEIW+AAR+TYSS EN
Sbjct: 64  EISAPETTDPKYRAWRTDDHLVMSWLINSMTTEVGENFLLFRTAKEIWEAARETYSSTEN 123

Query: 433 SSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMALYKKIVEKKR 492
           SS L  IETRLYDLRQG+L+VTQYFN L R W  LDMYE Y WKC ++ ALYKKIVE+KR
Sbjct: 124 SSELFEIETRLYDLRQGELSVTQYFNTLTRCWLQLDMYEIYAWKCPDNSALYKKIVEQKR 183

Query: 493 ILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            ++FLL LNK+LD+VRGRIMG K LPS+R
Sbjct: 184 TIKFLLGLNKDLDEVRGRIMGIKPLPSIR 212

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BLAST of Lag0026523 vs. NCBI nr
Match: XP_023738515.1 (uncharacterized protein LOC111886493 [Lactuca sativa])

HSP 1 Score: 268.1 bits (684), Expect = 1.6e-67
Identity = 121/181 (66.85%), Postives = 151/181 (83.43%), Query Frame = 0


Query: 339 TNHKLQGHNFLQWNQSVFIYICGRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLN 398
           T+HKL G NF QW+QSVF++ICGR KDGHLTG+T A D KDPKFR+W+T+DHLVMSWL+N
Sbjct: 29  TSHKLNGQNFFQWSQSVFMFICGRDKDGHLTGETVAADSKDPKFRTWRTNDHLVMSWLIN 88

Query: 399 SMTPEVGENFLLFKTTKEIWDAARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLL 458
           SMT EVGENFLL+KT +EIW+AA++TYSS ENSS L  +ET+LYDLRQGDL+VTQYF+LL
Sbjct: 89  SMTTEVGENFLLYKTAQEIWEAAKETYSSTENSSELFELETKLYDLRQGDLSVTQYFHLL 148

Query: 459 VRNWQHLDMYETYEWKCGEDMALYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSL 518
            R W  LD++ET+ WKC +D+A Y+ +V +KR +RFLL LNK+LD VR R+MG   LP+ 
Sbjct: 149 SRIWLQLDLFETHPWKCVDDVASYRSVVNQKRTIRFLLGLNKDLDGVRSRVMGTHPLPTP 208

Query: 519 R 520
           R
Sbjct: 209 R 209

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BLAST of Lag0026523 vs. NCBI nr
Match: XP_034898954.1 (uncharacterized protein LOC118037156 [Populus alba])

HSP 1 Score: 263.8 bits (673), Expect = 3.0e-66
Identity = 119/189 (62.96%), Postives = 154/189 (81.48%), Query Frame = 0


Query: 331 QDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTGDTPAPDVKDPKFRSWKTDDH 390
           + +SHQI T HKL G+N+LQW+ SV ++ICG+G+D +LTGD   P+  DP FR+WKT++H
Sbjct: 29  EGTSHQI-TGHKLNGYNYLQWSSSVMMFICGKGRDDYLTGDIIIPERNDPMFRTWKTENH 88

Query: 391 LVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFENSSALLAIETRLYDLRQGDLN 450
           +VMSWL+NSMT E+GENFLL+ T KEIW+AAR+TYSS EN+S L  IE  L+DLRQG+L+
Sbjct: 89  MVMSWLINSMTNEIGENFLLYGTAKEIWEAARETYSSSENTSELFEIEAVLHDLRQGELS 148

Query: 451 VTQYFNLLVRNWQHLDMYETYEWKCGEDMALYKKIVEKKRILRFLLRLNKELDDVRGRIM 510
           +TQ+FN L R+WQHLDM+ET+ W C ED  LY++IVE+KR  +FLL LNK LD+VRGRIM
Sbjct: 149 ITQFFNTLCRHWQHLDMFETHSWNCPEDTVLYRRIVEQKRTFKFLLGLNKNLDEVRGRIM 208

Query: 511 GAKDLPSLR 520
           G K LP+LR
Sbjct: 209 GTKPLPNLR 216

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BLAST of Lag0026523 vs. NCBI nr
Match: RVX18965.1 (hypothetical protein CK203_007146 [Vitis vinifera])

HSP 1 Score: 260.8 bits (665), Expect = 2.6e-65
Identity = 121/219 (55.25%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 314 MAKHGLAS--VSYENSPQP-----------QDSSHQIVTNHKLQGHNFLQWNQSVFIYIC 373
           M K+G+AS  VS   SP+             DSS  ++T HKL GHN+LQW+QSV ++IC
Sbjct: 231 MTKYGMASSQVSSVTSPESGGRSEIPNLGGSDSSPILITGHKLNGHNYLQWSQSVLLFIC 290

Query: 374 GRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDA 433
           G+GKD +LTG+   P+  +P FR WK ++ ++MSWL+NSM  ++GENFLLF T K+IWDA
Sbjct: 291 GKGKDEYLTGEAAMPETTEPGFRKWKIENSMIMSWLINSMNNDIGENFLLFGTAKDIWDA 350

Query: 434 ARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMA 493
           A++TYSS EN+S L  +E+ L+D RQG+ +VTQY+N L R WQ LD++ET+ WKC +D A
Sbjct: 351 AKETYSSSENTSELFQVESALHDFRQGEQSVTQYYNTLTRYWQQLDLFETHSWKCSDDAA 410

Query: 494 LYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            Y+KIVE+KR+ +F L LN+ELDDVRGRIMG K LPSLR
Sbjct: 411 TYRKIVEQKRLFKFFLGLNRELDDVRGRIMGIKPLPSLR 449

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BLAST of Lag0026523 vs. NCBI nr
Match: KAG5219081.1 (Retrovirus-related polyprotein from transposon [Salix suchowensis] >KAG5250156.1 Retrovirus-related polyprotein from transposon [Salix suchowensis])

HSP 1 Score: 260.0 bits (663), Expect = 4.4e-65
Identity = 117/193 (60.62%), Postives = 155/193 (80.31%), Query Frame = 0


Query: 327 SPQPQDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTGDTPAPDVKDPKFRSWK 386
           SP  ++SS  ++T HKL G+N+LQW+ SV ++ICG+G+D +LTG+   P  +DP+FR+WK
Sbjct: 24  SPGTENSS-LLITCHKLNGYNYLQWSSSVMMFICGKGRDDYLTGEATMPGKEDPQFRNWK 83

Query: 387 TDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFENSSALLAIETRLYDLRQ 446
           T++H++MSWL+NSMT E+GENFLL+ T KEIWDAAR+TYS+ +N+S L  IE  L+DLRQ
Sbjct: 84  TENHMIMSWLINSMTNEIGENFLLYGTAKEIWDAARETYSNSDNTSELFEIEAALHDLRQ 143

Query: 447 GDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMALYKKIVEKKRILRFLLRLNKELDDVR 506
           G+LNVTQYFN L R+WQ LDM+E + WKC  D  L+++IVE+KR  +FLL LNK LD+VR
Sbjct: 144 GELNVTQYFNTLTRHWQQLDMFEVHTWKCSGDTVLFRRIVEQKRTFKFLLGLNKTLDEVR 203

Query: 507 GRIMGAKDLPSLR 520
           GRIMG K LPSLR
Sbjct: 204 GRIMGTKPLPSLR 215

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BLAST of Lag0026523 vs. ExPASy Swiss-Prot
Match: Q9LVP1 (DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.1e-37
Identity = 82/130 (63.08%), Postives = 93/130 (71.54%), Query Frame = 0


Query: 21  RLDARAARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGER 80
           R D RA  +V+EQ  H  WG+YAQRLL+P  GLWRNP+ GGHDDKAHPPIHPTKFS+GE 
Sbjct: 353 RTDLRA--MVEEQTRHPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGES 412

Query: 81  GWSQDHHSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRM 140
            WS+DH               +YELVVRH+LACVSQPAV A TTVEIDIAGE FS SGR 
Sbjct: 413 NWSRDH-------------LNVYELVVRHYLACVSQPAVAAETTVEIDIAGERFSASGRA 467

Query: 141 ILAVSSLHKF 151
           ILA + L  +
Sbjct: 473 ILAKNYLEVY 467

[Close]

BLAST of Lag0026523 vs. ExPASy Swiss-Prot
Match: C7J0A2 (DNA topoisomerase 3-alpha OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3A PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.0e-37
Identity = 79/119 (66.39%), Postives = 88/119 (73.95%), Query Frame = 0


Query: 29  IVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDHHS 88
           IV EQ  H  WG YAQRLLDP + LWRNPS GGHDDKAHPPIHPTKFSAGE  W+ +H  
Sbjct: 361 IVHEQVAHPNWGTYAQRLLDPEARLWRNPSNGGHDDKAHPPIHPTKFSAGETNWTDNH-- 420

Query: 89  SVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMILAVSSL 148
                      ++LYELVVRHFLAC SQPAVGA TTVEIDIAGE F+ SGR++LA + L
Sbjct: 421 -----------KKLYELVVRHFLACCSQPAVGAETTVEIDIAGEQFNASGRVVLAKNYL 466

[Close]

BLAST of Lag0026523 vs. ExPASy Swiss-Prot
Match: Q9NG98 (DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 3.2e-18
Identity = 53/115 (46.09%), Postives = 70/115 (60.87%), Query Frame = 0


Query: 27  ARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGERGWSQDH 86
           A +V+ Q  H+ WGA+AQR+++ G     NP  G   D+AHPPIHPTK +   +G     
Sbjct: 371 APLVEMQTGHRDWGAFAQRVIEWGP----NPRNGNKSDQAHPPIHPTKLAENLQG----- 430

Query: 87  HSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMI 142
                      +  R+YELVVRHFLACVS+ AVG+ T V IDIAGE F+ +G +I
Sbjct: 431 -----------NEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVI 465

[Close]

BLAST of Lag0026523 vs. ExPASy Swiss-Prot
Match: O70157 (DNA topoisomerase 3-alpha OS=Mus musculus OX=10090 GN=Top3a PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.7e-17
Identity = 53/121 (43.80%), Postives = 67/121 (55.37%), Query Frame = 0


Query: 23  DARAARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGERGW 82
           D     +V++Q     WGA+AQ +L+ G      P  G   D+AHPPIHPTK+++G +G 
Sbjct: 373 DLNLVALVEQQTVDPHWGAFAQTILERGG---PTPRNGSKSDQAHPPIHPTKYTSGLQG- 432

Query: 83  SQDHHSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMIL 142
                            +RLYE +VRHFLAC SQ A G  TTVEIDIA E F   G +IL
Sbjct: 433 ---------------DDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLIIL 474

Query: 143 A 144
           A
Sbjct: 493 A 474

[Close]

BLAST of Lag0026523 vs. ExPASy Swiss-Prot
Match: Q13472 (DNA topoisomerase 3-alpha OS=Homo sapiens OX=9606 GN=TOP3A PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.6e-17
Identity = 54/121 (44.63%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 23  DARAARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGERGW 82
           D     +V++Q     WGA+AQ +L+ G      P  G   D+AHPPIHPTK++   +G 
Sbjct: 373 DLNLTVLVEQQTPDPRWGAFAQSILERGG---PTPRNGNKSDQAHPPIHPTKYTNNLQG- 432

Query: 83  SQDHHSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRMIL 142
                            QRLYE +VRHFLAC SQ A G  TTVEIDIA E F   G MIL
Sbjct: 433 ---------------DEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMIL 474

Query: 143 A 144
           A
Sbjct: 493 A 474

[Close]

BLAST of Lag0026523 vs. ExPASy TrEMBL
Match: A0A2I0B7Z4 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash004935 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 5.7e-79
Identity = 145/209 (69.38%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 313 QMAKHGLASVSYENSPQP--QDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTG 372
           Q +   + S S  NS  P   D+S  +VTNHKL GHNFLQW+QSVF+YICGRGKDGHLTG
Sbjct: 4   QSSSSEVTSRSESNSSLPIVSDNSSHLVTNHKLHGHNFLQWSQSVFMYICGRGKDGHLTG 63

Query: 373 DTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFEN 432
           +  AP+  DPK+R+W+TDDHLVMSWL+NSMT EVGENFLLF+T KEIW+AAR+TYSS EN
Sbjct: 64  EISAPETTDPKYRAWRTDDHLVMSWLINSMTTEVGENFLLFRTAKEIWEAARETYSSTEN 123

Query: 433 SSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMALYKKIVEKKR 492
           SS L  IETRLYDLRQG+L+VTQYFN L R W  LDMYE Y WKC ++ ALYKKIVE+KR
Sbjct: 124 SSELFEIETRLYDLRQGELSVTQYFNTLTRCWLQLDMYEIYAWKCPDNSALYKKIVEQKR 183

Query: 493 ILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            ++FLL LNK+LD+VRGRIMG K LPS+R
Sbjct: 184 TIKFLLGLNKDLDEVRGRIMGIKPLPSIR 212

[Close]

BLAST of Lag0026523 vs. ExPASy TrEMBL
Match: A0A438KCP1 (Retrotrans_gag domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_007146 PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.2e-65
Identity = 121/219 (55.25%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 314 MAKHGLAS--VSYENSPQP-----------QDSSHQIVTNHKLQGHNFLQWNQSVFIYIC 373
           M K+G+AS  VS   SP+             DSS  ++T HKL GHN+LQW+QSV ++IC
Sbjct: 231 MTKYGMASSQVSSVTSPESGGRSEIPNLGGSDSSPILITGHKLNGHNYLQWSQSVLLFIC 290

Query: 374 GRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDA 433
           G+GKD +LTG+   P+  +P FR WK ++ ++MSWL+NSM  ++GENFLLF T K+IWDA
Sbjct: 291 GKGKDEYLTGEAAMPETTEPGFRKWKIENSMIMSWLINSMNNDIGENFLLFGTAKDIWDA 350

Query: 434 ARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMA 493
           A++TYSS EN+S L  +E+ L+D RQG+ +VTQY+N L R WQ LD++ET+ WKC +D A
Sbjct: 351 AKETYSSSENTSELFQVESALHDFRQGEQSVTQYYNTLTRYWQQLDLFETHSWKCSDDAA 410

Query: 494 LYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            Y+KIVE+KR+ +F L LN+ELDDVRGRIMG K LPSLR
Sbjct: 411 TYRKIVEQKRLFKFFLGLNRELDDVRGRIMGIKPLPSLR 449

[Close]

BLAST of Lag0026523 vs. ExPASy TrEMBL
Match: A5B2V6 (Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_031400 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.8e-65
Identity = 120/219 (54.79%), Postives = 160/219 (73.06%), Query Frame = 0


Query: 314 MAKHGLAS--VSYENSPQP-----------QDSSHQIVTNHKLQGHNFLQWNQSVFIYIC 373
           M K+G+AS  VS   SP+             DSS  ++T HKL GHN+LQW+QSV ++IC
Sbjct: 198 MTKYGMASSQVSSVTSPESGGRSKIPNLGGSDSSPILITRHKLNGHNYLQWSQSVLLFIC 257

Query: 374 GRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDA 433
           G+GKD +LTG+   P++  P FR WK ++ ++MSWL+NSM  ++GENFLLF+T K+IWD 
Sbjct: 258 GKGKDEYLTGEAAMPEITKPGFRKWKIENSMIMSWLINSMNNDIGENFLLFRTAKDIWDV 317

Query: 434 ARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMA 493
           A++TYSS EN S L  +E+ L+D RQG+ +VTQY+N L R WQ LD++ET+ WKC +D A
Sbjct: 318 AKETYSSSENISELFQVESALHDFRQGEQSVTQYYNTLTRYWQQLDLFETHSWKCSDDAA 377

Query: 494 LYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            Y+KIVE+KR+ +F L LN+ELDDVRGRIMG K LPSLR
Sbjct: 378 TYRKIVEQKRLFKFFLGLNRELDDVRGRIMGIKPLPSLR 416

[Close]

BLAST of Lag0026523 vs. ExPASy TrEMBL
Match: A0A438KNE1 (Copia protein OS=Vitis vinifera OX=29760 GN=GIP_350 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.8e-65
Identity = 120/219 (54.79%), Postives = 162/219 (73.97%), Query Frame = 0


Query: 314 MAKHGLAS--VSYENSPQP-----------QDSSHQIVTNHKLQGHNFLQWNQSVFIYIC 373
           M K+G+AS  VS   SP+             DSS  ++T HKL GHN+LQW+QSV ++IC
Sbjct: 1   MTKYGMASSQVSSVTSPESGGRSEIPNLGGNDSSPILITGHKLNGHNYLQWSQSVLLFIC 60

Query: 374 GRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDA 433
           G+GKD +LTG+   P+  +P FR WK +++++MSWL+NSM  ++GENFLLF T K+IWDA
Sbjct: 61  GKGKDEYLTGEAVMPETTEPGFRKWKIENNMIMSWLINSMNNDIGENFLLFGTAKDIWDA 120

Query: 434 ARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMA 493
           A++TYSS EN+S L  +E+ L+D RQG+ +VTQY+N L R WQ LD++ET+ WKC +D A
Sbjct: 121 AKETYSSSENTSELFQVESALHDFRQGEQSVTQYYNTLTRYWQQLDLFETHSWKCSDDAA 180

Query: 494 LYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            Y++IVE+KR+ +F L LN+ELDDVRGRIMG K LPSLR
Sbjct: 181 TYRQIVEQKRLFKFFLGLNRELDDVRGRIMGIKPLPSLR 219

[Close]

BLAST of Lag0026523 vs. ExPASy TrEMBL
Match: A0A438IAX1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1120 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 4.7e-65
Identity = 120/219 (54.79%), Postives = 161/219 (73.52%), Query Frame = 0


Query: 314 MAKHGLAS--VSYENSPQP-----------QDSSHQIVTNHKLQGHNFLQWNQSVFIYIC 373
           M K+G+AS  VS   SP+             DSS  ++T HKL GHN+LQW+QSV ++IC
Sbjct: 157 MTKYGMASSQVSSVTSPESGGRSEIPNLGGNDSSPILITGHKLNGHNYLQWSQSVLLFIC 216

Query: 374 GRGKDGHLTGDTPAPDVKDPKFRSWKTDDHLVMSWLLNSMTPEVGENFLLFKTTKEIWDA 433
           G+GKD +LTG+   P+  +P FR WK ++ ++MSWL+NSM  ++GENFLLF T K+IWDA
Sbjct: 217 GKGKDEYLTGEAVMPETTEPGFRKWKIENSMIMSWLINSMNNDIGENFLLFGTAKDIWDA 276

Query: 434 ARDTYSSFENSSALLAIETRLYDLRQGDLNVTQYFNLLVRNWQHLDMYETYEWKCGEDMA 493
           A++TYSS EN+S L  +E+ L+D RQG+ +VTQY+N L R WQ LD++ET+ WKC +D A
Sbjct: 277 AKETYSSSENTSELFQVESALHDFRQGEQSVTQYYNTLTRYWQQLDLFETHSWKCSDDAA 336

Query: 494 LYKKIVEKKRILRFLLRLNKELDDVRGRIMGAKDLPSLR 520
            Y++IVE+KR+ +F L LN+ELDDVRGRIMG K LPSLR
Sbjct: 337 TYRQIVEQKRLFKFFLGLNRELDDVRGRIMGIKPLPSLR 375

[Close]

BLAST of Lag0026523 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 159.5 bits (402), Expect = 7.5e-39
Identity = 82/130 (63.08%), Postives = 93/130 (71.54%), Query Frame = 0


Query: 21  RLDARAARIVQEQQEHQIWGAYAQRLLDPGSGLWRNPSGGGHDDKAHPPIHPTKFSAGER 80
           R D RA  +V+EQ  H  WG+YAQRLL+P  GLWRNP+ GGHDDKAHPPIHPTKFS+GE 
Sbjct: 353 RTDLRA--MVEEQTRHPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGES 412

Query: 81  GWSQDHHSSVINNSEIFSSQRLYELVVRHFLACVSQPAVGAVTTVEIDIAGELFSTSGRM 140
            WS+DH               +YELVVRH+LACVSQPAV A TTVEIDIAGE FS SGR 
Sbjct: 413 NWSRDH-------------LNVYELVVRHYLACVSQPAVAAETTVEIDIAGERFSASGRA 467

Query: 141 ILAVSSLHKF 151
           ILA + L  +
Sbjct: 473 ILAKNYLEVY 467

[Close]

BLAST of Lag0026523 vs. TAIR 10
Match: AT1G21280.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Has 707 Blast hits to 705 proteins in 25 species: Archae - 0; Bacteria - 0; Metazoa - 4; Fungi - 0; Plants - 703; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 77.8 bits (190), Expect = 2.9e-14
Identity = 58/196 (29.59%), Postives = 95/196 (48.47%), Query Frame = 0


Query: 330 PQDSSHQIVTNHKLQGHNFLQWNQSVFIYICGRGKDGHLTGDTPAPDVKDPKFRSWKTDD 389
           P D S Q ++  +    N++ W      ++    K G + G  P PD   P ++ W+  +
Sbjct: 27  PSDFSIQKLSKDE---DNYVAWKIRFRSFLRVTKKFGFIDGTLPKPDPFSPLYQPWEQCN 86

Query: 390 HLVMSWLLNSMTPEVGENFLLFKTTKEIWDAARDTYSSFENSSALLAIETRLYDLRQGDL 449
            +VM WL+NSMT ++ E+ +  +T  ++W+  R  +    +   +  +  RL  LRQG  
Sbjct: 87  AMVMYWLMNSMTDKLLESVMYAETAHKMWEDLRRVFVPCVDLK-IYQLRRRLATLRQGGD 146

Query: 450 NVTQYFNLLVRNWQHLDMYETY-EWKCG----EDMALYKKIVEKKRILRFL--LRLNKEL 509
           +V +YF  L + W  L  Y    E KCG    E     ++  EK++   FL  L+LN+  
Sbjct: 147 SVEEYFGKLSKVWMELSEYAPIPECKCGGCNCECTKRAEEAREKEQRYEFLMGLKLNQGF 206

Query: 510 DDVRGRIMGAKDLPSL 519
           + V  +IM  K  PSL
Sbjct: 207 EAVTTKIMFQKPPPSL 218

The following BLAST results are available for this feature:

BLAST of Lag0026523 vs. NCBI nr


Match Name	E-value	Identity	Description
PKA63925.1	1.2e-78	69.38	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Apostasia shenzheni...	[more]
XP_023738515.1	1.6e-67	66.85	uncharacterized protein LOC111886493 [Lactuca sativa]	[more]
XP_034898954.1	3.0e-66	62.96	uncharacterized protein LOC118037156 [Populus alba]	[more]
RVX18965.1	2.6e-65	55.25	hypothetical protein CK203_007146 [Vitis vinifera]	[more]
KAG5219081.1	4.4e-65	60.62	Retrovirus-related polyprotein from transposon [Salix suchowensis] >KAG5250156.1...	[more]

BLAST of Lag0026523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVP1	1.1e-37	63.08	DNA topoisomerase 3-alpha OS=Arabidopsis thaliana OX=3702 GN=TOP3A PE=1 SV=1	[more]
C7J0A2	4.0e-37	66.39	DNA topoisomerase 3-alpha OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3A PE=3...	[more]
Q9NG98	3.2e-18	46.09	DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 S...	[more]
O70157	2.7e-17	43.80	DNA topoisomerase 3-alpha OS=Mus musculus OX=10090 GN=Top3a PE=2 SV=1	[more]
Q13472	4.6e-17	44.63	DNA topoisomerase 3-alpha OS=Homo sapiens OX=9606 GN=TOP3A PE=1 SV=1	[more]

BLAST of Lag0026523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2I0B7Z4	5.7e-79	69.38	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Apostasia shenzhe...	[more]
A0A438KCP1	1.2e-65	55.25	Retrotrans_gag domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_007...	[more]
A5B2V6	2.8e-65	54.79	Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITI...	[more]
A0A438KNE1	2.8e-65	54.79	Copia protein OS=Vitis vinifera OX=29760 GN=GIP_350 PE=4 SV=1	[more]
A0A438IAX1	4.7e-65	54.79	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]

BLAST of Lag0026523 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G63920.1	7.5e-39	63.08	topoisomerase 3alpha	[more]
AT1G21280.1	2.9e-14	29.59	CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Ha...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013497	DNA topoisomerase, type IA, central	PFAM	PF01131	Topoisom_bac	coord: 28..142 e-value: 2.4E-12 score: 46.6
IPR013826	DNA topoisomerase, type IA, central region, subdomain 3	GENE3D	1.10.290.10	Topoisomerase I, domain 4	coord: 17..116 e-value: 1.3E-9 score: 40.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..82
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 168..187
None	No IPR available	PANTHER	PTHR37610:SF47	SUBFAMILY NOT NAMED	coord: 328..509
None	No IPR available	PANTHER	PTHR37610	FAMILY NOT NAMED	coord: 328..509
IPR023405	DNA topoisomerase, type IA, core domain	SUPERFAMILY	56712	Prokaryotic type I DNA topoisomerase	coord: 25..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026523.1	Lag0026523.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006265	DNA topological change
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003916	DNA topoisomerase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0008270	zinc ion binding