Lag0026195 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0026195
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-locus lectin protein kinase family protein, putative
Locationchr10: 31935946 .. 31936548 (+)
RNA-Seq ExpressionLag0026195
SyntenyLag0026195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGGAGCGGAGGTGGTGAGATTCAGGACCACCGATCTGTTTGTAGACTACAGGGCGTGGCACACTTTCCTCTGCGCCAAGTTGATGCCTGTGGCGCATCTAAGCGATGTTACCAAGAGTCGTGCCATCCTTCTATTCGCTATCGCCACAGGCCGTTCGGTGAATGTTGGGCAGGTTATTCATCAATCTATGCGCCACATCCGCCGTCGTAGCACGACAGTAGGGCTCGGGCATCCATCACTGATCACAACACTATGCCGAGCCGCTGGTGTCGTGTGGGACGCCCAGGAGGAGTTGGTCCACCCCGGGGCGATTATAGACAAGAATTTCATACGTCGCTACCGAGGGCCTGGACCACAGGGTGCCCAGCTAGTTTTCCCCATCCATGCAAAGCCAGAGCATCCTGAGCAGCATGCAGAGCCTGAGCAGCAGGAGCAAGAGGTTCCACCTCCCACCATCGAGGAGCAGCTACAGCAGCTGCGTATGGAGTTCAAGAGTCACAGGCTGGAGTTCCAGAGTCATCGGCAGGAGCTCCAGAGTCAGCAGCGCACGCACCAGAGAGAGAGGCGTAGGGATTGTCTGTTGGGTTTTATGCCCTAA

mRNA sequence

ATGACAGGAGCGGAGGTGGTGAGATTCAGGACCACCGATCTGTTTGTAGACTACAGGGCGTGGCACACTTTCCTCTGCGCCAAGTTGATGCCTGTGGCGCATCTAAGCGATGTTACCAAGAGTCGTGCCATCCTTCTATTCGCTATCGCCACAGGCCGTTCGGTGAATGTTGGGCAGGTTATTCATCAATCTATGCGCCACATCCGCCGTCGTAGCACGACAGTAGGGCTCGGGCATCCATCACTGATCACAACACTATGCCGAGCCGCTGGTGTCGTGTGGGACGCCCAGGAGGAGTTGGTCCACCCCGGGGCGATTATAGACAAGAATTTCATACGTCGCTACCGAGGGCCTGGACCACAGGGTGCCCAGCTAGTTTTCCCCATCCATGCAAAGCCAGAGCATCCTGAGCAGCATGCAGAGCCTGAGCAGCAGGAGCAAGAGGTTCCACCTCCCACCATCGAGGAGCAGCTACAGCAGCTGCGTATGGAGTTCAAGAGTCACAGGCTGGAGTTCCAGAGTCATCGGCAGGAGCTCCAGAGTCAGCAGCGCACGCACCAGAGAGAGAGGCGTAGGGATTGTCTGTTGGGTTTTATGCCCTAA

Coding sequence (CDS)

ATGACAGGAGCGGAGGTGGTGAGATTCAGGACCACCGATCTGTTTGTAGACTACAGGGCGTGGCACACTTTCCTCTGCGCCAAGTTGATGCCTGTGGCGCATCTAAGCGATGTTACCAAGAGTCGTGCCATCCTTCTATTCGCTATCGCCACAGGCCGTTCGGTGAATGTTGGGCAGGTTATTCATCAATCTATGCGCCACATCCGCCGTCGTAGCACGACAGTAGGGCTCGGGCATCCATCACTGATCACAACACTATGCCGAGCCGCTGGTGTCGTGTGGGACGCCCAGGAGGAGTTGGTCCACCCCGGGGCGATTATAGACAAGAATTTCATACGTCGCTACCGAGGGCCTGGACCACAGGGTGCCCAGCTAGTTTTCCCCATCCATGCAAAGCCAGAGCATCCTGAGCAGCATGCAGAGCCTGAGCAGCAGGAGCAAGAGGTTCCACCTCCCACCATCGAGGAGCAGCTACAGCAGCTGCGTATGGAGTTCAAGAGTCACAGGCTGGAGTTCCAGAGTCATCGGCAGGAGCTCCAGAGTCAGCAGCGCACGCACCAGAGAGAGAGGCGTAGGGATTGTCTGTTGGGTTTTATGCCCTAA

Protein sequence

MTGAEVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQSMRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPGPQGAQLVFPIHAKPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQELQSQQRTHQRERRRDCLLGFMP
Homology
BLAST of Lag0026195 vs. NCBI nr
Match: KAA0033353.1 (putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa] >TYJ96637.1 putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-25
Identity = 86/195 (44.10%), Postives = 114/195 (58.46%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VIH+S
Sbjct: 154 EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIHKS 213

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DKNFI    G      G
Sbjct: 214 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFIMGIPGWSFETMG 273

Query: 125 A----QLVFPIHA-KPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQ 184
           A    +     H  K      H EP  Q++  P   I E  Q L ++         S  +
Sbjct: 274 AGHCDETAGASHCNKTPDAGHHDEPSDQDEAEP---IREVRQTLTIDLPRQTQRPLSLDE 333

Query: 185 ELQSQQRTHQRERRR 193
           ++Q  +R  +   RR
Sbjct: 334 QIQRLERRVRSYHRR 345

BLAST of Lag0026195 vs. NCBI nr
Match: XP_008458668.1 (PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo])

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-25
Identity = 86/195 (44.10%), Postives = 114/195 (58.46%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VIH+S
Sbjct: 92  EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIHKS 151

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DKNFI    G      G
Sbjct: 152 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFIMGIPGWSFETMG 211

Query: 125 A----QLVFPIHA-KPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQ 184
           A    +     H  K      H EP  Q++  P   I E  Q L ++         S  +
Sbjct: 212 AGHCDETAGASHCNKTPDAGHHDEPSDQDEAEP---IREVRQTLTIDLPRQTQRPLSLDE 271

Query: 185 ELQSQQRTHQRERRR 193
           ++Q  +R  +   RR
Sbjct: 272 QIQRLERRVRSYHRR 283

BLAST of Lag0026195 vs. NCBI nr
Match: KGN46897.1 (hypothetical protein Csa_020731 [Cucumis sativus])

HSP 1 Score: 120.9 bits (302), Expect = 1.2e-23
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VI +S
Sbjct: 154 EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIQKS 213

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DK+FI    G    P G
Sbjct: 214 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKSFIMEIPGWSFEPMG 273

Query: 125 A----QLVFPIHAKPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQE 184
           A    +     H        H +    + E  P  I E  Q L ++         S  ++
Sbjct: 274 AGHCDETAGTSHCNKTTDAGHNDEPSDQDEAEP--IREVRQTLTIDLPRQTQRPLSLDEQ 333

Query: 185 LQSQQRTHQRERRR 193
           ++  +R  +   RR
Sbjct: 334 IRRLERRVRSYHRR 345

BLAST of Lag0026195 vs. NCBI nr
Match: EOY01634.1 (Uncharacterized protein TCM_011481 [Theobroma cacao])

HSP 1 Score: 102.1 bits (253), Expect = 5.9e-18
Identity = 49/116 (42.24%), Postives = 77/116 (66.38%), Query Frame = 0

Query: 7   VRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQSMR 66
           V F+   L   ++ W+ FL A+L+PV H+S +TK RA+LL+A+ TG+++NVG++I +++ 
Sbjct: 85  VSFKANALNKFFKVWYHFLTARLLPVKHVSVITKDRAVLLYAMVTGKTINVGKLIFENIL 144

Query: 67  HIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRR---YRGPG 120
           H+   S   G+ +PSLIT LC+ A V W + EEL+HP   +D N + R   Y+ PG
Sbjct: 145 HV-AGSAKEGIWYPSLITALCKQARVQWSSVEELLHPKVPLDANIVNRLYNYQPPG 199

BLAST of Lag0026195 vs. NCBI nr
Match: EOY08722.1 (Uncharacterized protein TCM_023809 [Theobroma cacao])

HSP 1 Score: 99.8 bits (247), Expect = 2.9e-17
Identity = 47/99 (47.47%), Postives = 68/99 (68.69%), Query Frame = 0

Query: 17  DYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQSMRHIRRRSTTVG 76
           +Y+ W+ FL AK++P+ HLSDVTK RA+LL+AI  G+S+++GQ+I  S+ H   RS   G
Sbjct: 4   EYKVWYHFLKAKMLPIKHLSDVTKDRAMLLYAIILGKSIDIGQLIFNSIVH-TTRSIRDG 63

Query: 77  LGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRY 116
           L +PS IT LC+  G+ W + EEL+H    +DK  I R+
Sbjct: 64  LWYPSFITGLCKQVGLQWTSNEELLHLIVPLDKGIIHRF 101

BLAST of Lag0026195 vs. ExPASy TrEMBL
Match: A0A5D3BBY3 (Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00020 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.4e-25
Identity = 86/195 (44.10%), Postives = 114/195 (58.46%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VIH+S
Sbjct: 154 EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIHKS 213

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DKNFI    G      G
Sbjct: 214 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFIMGIPGWSFETMG 273

Query: 125 A----QLVFPIHA-KPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQ 184
           A    +     H  K      H EP  Q++  P   I E  Q L ++         S  +
Sbjct: 274 AGHCDETAGASHCNKTPDAGHHDEPSDQDEAEP---IREVRQTLTIDLPRQTQRPLSLDE 333

Query: 185 ELQSQQRTHQRERRR 193
           ++Q  +R  +   RR
Sbjct: 334 QIQRLERRVRSYHRR 345

BLAST of Lag0026195 vs. ExPASy TrEMBL
Match: A0A1S3C7Y0 (uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.4e-25
Identity = 86/195 (44.10%), Postives = 114/195 (58.46%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VIH+S
Sbjct: 92  EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIHKS 151

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DKNFI    G      G
Sbjct: 152 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFIMGIPGWSFETMG 211

Query: 125 A----QLVFPIHA-KPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQ 184
           A    +     H  K      H EP  Q++  P   I E  Q L ++         S  +
Sbjct: 212 AGHCDETAGASHCNKTPDAGHHDEPSDQDEAEP---IREVRQTLTIDLPRQTQRPLSLDE 271

Query: 185 ELQSQQRTHQRERRR 193
           ++Q  +R  +   RR
Sbjct: 272 QIQRLERRVRSYHRR 283

BLAST of Lag0026195 vs. ExPASy TrEMBL
Match: A0A0A0KER1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 5.9e-24
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0

Query: 5   EVVRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQS 64
           E +RF++++L V  + WH F+CAKL+PVAH S VTK RAILL+AIAT RSV+VG+VI +S
Sbjct: 154 EPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGKVIQKS 213

Query: 65  MRHIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRRYRGPG--PQG 124
           + +IR+   T GLGH SLIT LCR  GVVW+ +EELV P  I+DK+FI    G    P G
Sbjct: 214 LCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKSFIMEIPGWSFEPMG 273

Query: 125 A----QLVFPIHAKPEHPEQHAEPEQQEQEVPPPTIEEQLQQLRMEFKSHRLEFQSHRQE 184
           A    +     H        H +    + E  P  I E  Q L ++         S  ++
Sbjct: 274 AGHCDETAGTSHCNKTTDAGHNDEPSDQDEAEP--IREVRQTLTIDLPRQTQRPLSLDEQ 333

Query: 185 LQSQQRTHQRERRR 193
           ++  +R  +   RR
Sbjct: 334 IRRLERRVRSYHRR 345

BLAST of Lag0026195 vs. ExPASy TrEMBL
Match: A0A0A0KNI1 (AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.5e-19
Identity = 54/89 (60.67%), Postives = 67/89 (75.28%), Query Frame = 0

Query: 29  LMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQSMRHIRRRSTTVGLGHPSLITTLCR 88
           L+P+AH S VTK RAILL+AIAT RSV+VG+VI +S+ +IR+   T GLGH SLIT LCR
Sbjct: 149 LLPMAHTSSVTKERAILLYAIATKRSVDVGKVIQKSLCNIRKSGMTGGLGHSSLITALCR 208

Query: 89  AAGVVWDAQEELVHPGAIIDKNFIRRYRG 118
             GVVW+ +EELV P  I+DK+FI    G
Sbjct: 209 NEGVVWNEKEELVDPKPIMDKSFIMEIPG 237

BLAST of Lag0026195 vs. ExPASy TrEMBL
Match: A0A061EH53 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_011481 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.8e-18
Identity = 49/116 (42.24%), Postives = 77/116 (66.38%), Query Frame = 0

Query: 7   VRFRTTDLFVDYRAWHTFLCAKLMPVAHLSDVTKSRAILLFAIATGRSVNVGQVIHQSMR 66
           V F+   L   ++ W+ FL A+L+PV H+S +TK RA+LL+A+ TG+++NVG++I +++ 
Sbjct: 85  VSFKANALNKFFKVWYHFLTARLLPVKHVSVITKDRAVLLYAMVTGKTINVGKLIFENIL 144

Query: 67  HIRRRSTTVGLGHPSLITTLCRAAGVVWDAQEELVHPGAIIDKNFIRR---YRGPG 120
           H+   S   G+ +PSLIT LC+ A V W + EEL+HP   +D N + R   Y+ PG
Sbjct: 145 HV-AGSAKEGIWYPSLITALCKQARVQWSSVEELLHPKVPLDANIVNRLYNYQPPG 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0033353.12.9e-2544.10putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa]... [more]
XP_008458668.12.9e-2544.10PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo][more]
KGN46897.11.2e-2341.75hypothetical protein Csa_020731 [Cucumis sativus][more]
EOY01634.15.9e-1842.24Uncharacterized protein TCM_011481 [Theobroma cacao][more]
EOY08722.12.9e-1747.47Uncharacterized protein TCM_023809 [Theobroma cacao][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BBY31.4e-2544.10Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuw... [more]
A0A1S3C7Y01.4e-2544.10uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=... [more]
A0A0A0KER15.9e-2441.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1[more]
A0A0A0KNI11.5e-1960.67AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 P... [more]
A0A061EH532.8e-1842.24Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_011481 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 158..192
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0026195.1Lag0026195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006561 proline biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004349 glutamate 5-kinase activity