Lag0025064 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0025064
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMagnesium transporter CorA-like family protein
Locationchr10: 8228202 .. 8233303 (+)
RNA-Seq ExpressionLag0025064
SyntenyLag0025064
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTCCACCATAGCTCTCATGCAGATAGTGTGGAGAAGCCAGAAGCTCCCAAACCCTTTAACAAGGATGCCACACTTGGATGTGATCTGTGGACAGATGGGCTTATTTGTGCTTTTGAGTATATTGGGAGACCAAAGAAATCAATCGATTCAAAATCCAGATCCAAGATGCCTGATAGGCAACAAATGAATAGTGAATTTCCTACCACGCGGATACCTACAACTGAACTGAATAATGGCAATGCCATTGCACGCGAGAGGAAAAGGTTCCTAGAACCCACATCCTCGGATGATCTTAAGAGCCAATGTGGCCAATTTTGTGAAAGAGAAAAAGTTGAGAACCATAGTTGGTTACCTATAGGATGGGACAGAATATCAGAGCTGGTTCAAACAATACAAGTAGATGCTGAATGGGCCTCAATGTCATGTGATTTCATGGACGAAGAAGATGACCTTACAGTGGCAGATCTTGTAGCACCTTATTGGAAGCGTCCAGCTGGACCTATATGGTGGTGCCATGTCTCTGCAAGCCACCCTTCTGTTGAAGCGTGGCTCAGAAGTGCTCATTGGTTGCATCCTGCCATTAGTTTGGCTTTAAGAGATGAAAGTCGACTGATAAGTGACCGCATGAAGCACCTTTTCTATGAGGTGGAAATCTCCCTAGTTTTCAGGCTACTTGAGTGCTAATGTATATATAGTAATAGGAAGCATTTTTTTTTGCTAACAGAGAATTAGTTACAAGAAGAAATTTCATTGTTAATGTTGTTAAGTAACATGGGATACATGATAAGTGTGCACATGCTGATTGGAAAACTTTCACAATGGTTCAATTTTTTTGCAAGGGGAAACAATTCATTGTTTCTTTGCATATCAATATATTTCCTCGTGAAATTGTCATTTTTTACACCTGATTTAGCCATGTCAGTTTGGTGCAATTTTCTCTTGGTTTATTTCTTATACATTCTCTATTCGTAGAACATGGTTGGACCAAAATTATCTTTAACAAGAAGATAAAGCAGTGTAGACTTGGGCTTTAGGACACGTCTAGAAACTATTTTAAAACAATTTTCATTTTTTAAAACAAAAGGTCAAAGAAAATATGTTTGGACAAAGCAGTACTCTTGCAGTCTTGTCTATTAATCATGGTTTCAGATTTTCATGGATATTCTCTGAGTTACTAACCACTTTTTATTTTTATTTTTTTATTTTTTTTATTTCTTAATAATAATAATTATTTTTACTTCTAGACAGGTTCCTGTAAGGGTGGCTGGAGGACTGCTATTTGAACTACTAGGACAATCTGCTGGAGATCCATCGAGTGACGAGGATGACATTCCCATTGTACTTCGGTCTTGGCAAGCTCAGAACTTCCTGATAACGTCATTGCATGTTAAAGGACCAATAACAAACATAAATGTTTTAGGCATCACAGAGGTTCAGGTAACATTCCATGCAAGAAATAATTATTTACTCCGTGCTTGAAAACTTGGACAATTTTATTATTATTTTTCTGATTTCTTCTTCAACCTCTGGTTAGGAGCTTCTGATAATAGGAGATTATAATGCACCAAGAACAGTGCATGAAGTTATAGCACAACTAGCTTGCCGTCTTACAAGATGGGATGATAGGTAACAACTCCATTTTAAAAAGTTAACACTGCAGTTGCATAGTTGACCCTAGAATAATTATTATTCATGGACAAAATCAATATTCCTTCGACATATTGTCGACCATATGAGTGGCCTAATTTCCTTTCTTATTGAACTGTGCGTGCGTGCATAAATAATCTATTAATCTGTATGGTTGACAAAAGTTACCAGGATGATTTATTCAAATACCGTGCATTCTCCTTTAAAGGTTCATGATAGTTATATGCCAAGAGGTCCAAGACCAATAGGCATCTTTCTTGAGTTGAGTCTTATTTCTGCAGTCAAATCTTCCTCAATACTATGACTGATGTATTAAATTCAATTATCCCTTTTCTTACAGGTTATTTCGAAAATCCATATTTGGAGCAGCAGATGAGATTGAATTGAAGTTTATGGACAGGTTCTTATCTTGCATGTCATCTATTTAAGTGATATCATCTTGATTTATCTATTTTATCTTCATTTTTACGAACACTCTCCTTCACTTTCCCCCTCGTAAATATAGGAGAAACCATGAAGATATGCATCTTTTTAGTATAATCTTGAATCAAGAAATCAGAAAATTGTCAAGACAGGTATGCTTCCTCATCACTGTAATCATACTTGATGGTCGTGGAAAGTTTTCCTTGATGTATGTTGTTCTAGATAGTATTCCCATTAATTCAATCCTTGGATAATGATAGTTAGTTCAAAAGAGAGTATGAGGCATTTGCATGTGTAGAATTTTGTTTTAAATTTCATCTTCCTCTAGTTTGTTTGGTAAACGTTTTTTTCTTGGTTATGACCTTTTCCTCGAGGATACCTCCTCCTAATCATTATATCTTTTTTACTTTAGCTATACTGTTGTTTCCTATGAAAAATTTCGTCTGTAAGCGTATAGCATCAATCAGTACCCCTCACCTCAAGCTGATACCACTTTATTTTTTTTTTTTGAAAGAAATCTTTCTAATGTCTTCAGTTGCCGAACTCATTACCAATTTGGACCTTGCTTGTACAAAATGAGTCTCCTTCACAATTCTGTAAAAGCCTAAACAAATTAGATCAAATGTTGCTGTAGATTTTAATTAAATGTTTAGTTGACTTAAATATGCAAGAAGTCCAACGTGGGGGAGGGAATGGAAAGGGAAGAGGAGGATTCATATTTATTGAGATGTTACAATGATATACTGTCACTTTGGGACCAGGAGTCAAGGATAAGCTTCTTGTACTCCAATTCGTACTGTTCTCTTGTGTACCTCTAATGAAGTTGTTGATGAGCCGTTTCCAATTAACAAACACATTCAAACACATTTGTTGTCCCATATAGTAAAGGAGAAAAGACCCTTAGCACTCCTTTCTCCACTTCAAAGTAGTACTTTAGTGACAGTGAATTTTATTTTTATTTTCTTTGTCTTTTGGTTGATTAGAGGACGGAGTGATCTTGTGCATGTACTCTCTTCAGTCTTTTAAAACAAATATAGGAAATTTCAGGTTATCAGAGTAAAATGGTCGCTGCATGCGAGAGAGGAGATTATATATGAGCTTCTTCAGCATCTACGAGGATATGTGACAAAAAGCTTATTGGAGAGCATAAGAAAGAGTACTAGACAAATGATTGAAGAGCAAGAAGCAGTTCGTGATCGTCTATTCACCATTCAAGATGTCATGCAGAGTAATGTTCGTGCATGGTTACAGGTATTTGATTCATTGATAAATTAATGAGGATATTTTAGTGGCCATTTGGTATCTAACCTGTTTTATTTTTAATTTTTTATATTTGTTAATATAAGGGACACTGATCCTATAAATACACACACACATACATACATATATCAGGGACACTAAGAAATTAAGAATGCACTAAATTTGTGTTTGGCTAACTAAATTTCTGAAAATGATTTTAAAAAATAGTAATCTATGAAGCACGGACACGTCAAAATGGACGAAGGATCTGTGTCGGACACGTGTCGGACACGGATTCGTCCGGACACGCTCCGGACACGTGTCGGACACGCAATTTGCGTGTCCGAAAATATATTTTTTTTTTGAATTTCGGACACGCCGGGACACGTCCCGGACACGCCCCGGACACGCCCACATCAAATTTTTTAATTTTGGGCCGGCTGAATAAAAGCCCAATTCTCTGGGCACTGGGCTGGCAGGCTATATTTAATTTAAAAAAAAAAAAAAAGATAAATAGAATAAGTTGTTTGTAACTTGGTTGAATCAAGAATGTTGGTTAACTTAATGTATTTTGTGTTATTCTCACATCTTAATTGATTTGATATTTGATGTTTCATGTTTTTAGTGGTATACTTTTAGTATTCTTATGTTAAATTTATATATATACTAAAAAAAAAAAAAAAACAGCGTATCCTCAACGTATCCGTATCTTCGTTTTTTAGAAATTGGCGTATCGCCGTGTCCTGTCCTGTCCTATCCGTGTCCGTGTCCCGTGTCCGTGTCCGTGCTTCTTAGATAGTAATTTATGTGAGAACATCCTTTCAGTGTCTACCTTTTGGCAAATACATTGTTTCAAAATTGTTAACAAATGAAGTGGTGAGATTTTCGTGTTCTTGTAGATTATATCTTCACCCTTCCTCCCTCTCCAAATCTGCAATCAAAAACACGAAATCAGATTTGCAATCCCCAAATACAAATTTAGAAGTTGTTGATCTTACAAGTTTTAGTTCTATAGTTCTTTATGTTGCGCCATCAAAATCTGAAGTGGGTTTGAATAATAACATTTTTATACTTTTTATTTATTTTATGATAAGAAAAAACATATCAATTTGTTATTTTAAAAAATTAATTATACAAATACATTCTATTTTCAATGTTTTCAAAATTTACTGCCCAACCCAGGTTTCCGATTTACAATTCCTAAATTATAATAAATACAATCTTATTTTTTGTTGTATGCCTTTTTTCAATTCTTCTTATTTTTTCCTTTTGATGATGGATTATGAACTAAAAGGAGCCTTAGCCTTCGTCTATGAATCAGGCAAGTGGTCTGTCTAACTAAATGCTCTCATGATGAAAGTCTGATAGTTTTCACTTTCTTTCTTACAACAATTATTCAGGACAAAAGCCTCCGAGTAACTCATAACTTGGCGGTTTTTGGTGGCTGTGGCCTTGTTCTTTCCATTATAACTGGGCTGTTCGGAATCAACGTAGACGGGATCCCCGGGGCGCAGGATACACCATATGCATTTGGATTGTTTACAGCAATCCTATTCTTCTTAGGGATTGTACTGATGGGAGGGGGCGTGGTTTACCTCGGCTTGAAGAAGCCCATGGCAGAAGAGATGGTTGAATTCAGGAAGCTGGAACTGCAAGAGTTGGTTAAAATGTTTCAGCATGAGGCTGAAACTCATGCTCAGGTACGAAAATCTGTTACTCGCAATAATATACCTCCTACTGCAAGAGATATTTTCACAGCTGATGCGGATTATGTGCTCATCGAATGA

mRNA sequence

ATGGATGTCCACCATAGCTCTCATGCAGATAGTGTGGAGAAGCCAGAAGCTCCCAAACCCTTTAACAAGGATGCCACACTTGGATGTGATCTGTGGACAGATGGGCTTATTTGTGCTTTTGAGTATATTGGGAGACCAAAGAAATCAATCGATTCAAAATCCAGATCCAAGATGCCTGATAGGCAACAAATGAATAGTGAATTTCCTACCACGCGGATACCTACAACTGAACTGAATAATGGCAATGCCATTGCACGCGAGAGGAAAAGGTTCCTAGAACCCACATCCTCGGATGATCTTAAGAGCCAATGTGGCCAATTTTGTGAAAGAGAAAAAGTTGAGAACCATAGTTGGTTACCTATAGGATGGGACAGAATATCAGAGCTGGTTCAAACAATACAAGTAGATGCTGAATGGGCCTCAATGTCATGTGATTTCATGGACGAAGAAGATGACCTTACAGTGGCAGATCTTGTAGCACCTTATTGGAAGCGTCCAGCTGGACCTATATGGTGGTGCCATGTCTCTGCAAGCCACCCTTCTGTTGAAGCGTGGCTCAGAAGTGCTCATTGGTTGCATCCTGCCATTAGTTTGGCTTTAAGAGATGAAAGTCGACTGATAAGTGACCGCATGAAGCACCTTTTCTATGAGGTTCCTGTAAGGGTGGCTGGAGGACTGCTATTTGAACTACTAGGACAATCTGCTGGAGATCCATCGAGTGACGAGGATGACATTCCCATTGTACTTCGGTCTTGGCAAGCTCAGAACTTCCTGATAACGTCATTGCATGTTAAAGGACCAATAACAAACATAAATGTTTTAGGCATCACAGAGGTTCAGGAGCTTCTGATAATAGGAGATTATAATGCACCAAGAACAGTGCATGAAGTTATAGCACAACTAGCTTGCCGTCTTACAAGATGGGATGATAGGTTATTTCGAAAATCCATATTTGGAGCAGCAGATGAGATTGAATTGAAGTTTATGGACAGGAGAAACCATGAAGATATGCATCTTTTTAGTATAATCTTGAATCAAGAAATCAGAAAATTGTCAAGACAGGTTATCAGAGTAAAATGGTCGCTGCATGCGAGAGAGGAGATTATATATGAGCTTCTTCAGCATCTACGAGGATATGTGACAAAAAGCTTATTGGAGAGCATAAGAAAGAGTACTAGACAAATGATTGAAGAGCAAGAAGCAGTTCGTGATCGTCTATTCACCATTCAAGATGTCATGCAGAGTAATGTTCGTGCATGGTTACAGGACAAAAGCCTCCGAGTAACTCATAACTTGGCGGTTTTTGGTGGCTGTGGCCTTGTTCTTTCCATTATAACTGGGCTGTTCGGAATCAACGTAGACGGGATCCCCGGGGCGCAGGATACACCATATGCATTTGGATTGTTTACAGCAATCCTATTCTTCTTAGGGATTGTACTGATGGGAGGGGGCGTGGTTTACCTCGGCTTGAAGAAGCCCATGGCAGAAGAGATGGTTGAATTCAGGAAGCTGGAACTGCAAGAGTTGGTTAAAATGTTTCAGCATGAGGCTGAAACTCATGCTCAGGTACGAAAATCTGTTACTCGCAATAATATACCTCCTACTGCAAGAGATATTTTCACAGCTGATGCGGATTATGTGCTCATCGAATGA

Coding sequence (CDS)

ATGGATGTCCACCATAGCTCTCATGCAGATAGTGTGGAGAAGCCAGAAGCTCCCAAACCCTTTAACAAGGATGCCACACTTGGATGTGATCTGTGGACAGATGGGCTTATTTGTGCTTTTGAGTATATTGGGAGACCAAAGAAATCAATCGATTCAAAATCCAGATCCAAGATGCCTGATAGGCAACAAATGAATAGTGAATTTCCTACCACGCGGATACCTACAACTGAACTGAATAATGGCAATGCCATTGCACGCGAGAGGAAAAGGTTCCTAGAACCCACATCCTCGGATGATCTTAAGAGCCAATGTGGCCAATTTTGTGAAAGAGAAAAAGTTGAGAACCATAGTTGGTTACCTATAGGATGGGACAGAATATCAGAGCTGGTTCAAACAATACAAGTAGATGCTGAATGGGCCTCAATGTCATGTGATTTCATGGACGAAGAAGATGACCTTACAGTGGCAGATCTTGTAGCACCTTATTGGAAGCGTCCAGCTGGACCTATATGGTGGTGCCATGTCTCTGCAAGCCACCCTTCTGTTGAAGCGTGGCTCAGAAGTGCTCATTGGTTGCATCCTGCCATTAGTTTGGCTTTAAGAGATGAAAGTCGACTGATAAGTGACCGCATGAAGCACCTTTTCTATGAGGTTCCTGTAAGGGTGGCTGGAGGACTGCTATTTGAACTACTAGGACAATCTGCTGGAGATCCATCGAGTGACGAGGATGACATTCCCATTGTACTTCGGTCTTGGCAAGCTCAGAACTTCCTGATAACGTCATTGCATGTTAAAGGACCAATAACAAACATAAATGTTTTAGGCATCACAGAGGTTCAGGAGCTTCTGATAATAGGAGATTATAATGCACCAAGAACAGTGCATGAAGTTATAGCACAACTAGCTTGCCGTCTTACAAGATGGGATGATAGGTTATTTCGAAAATCCATATTTGGAGCAGCAGATGAGATTGAATTGAAGTTTATGGACAGGAGAAACCATGAAGATATGCATCTTTTTAGTATAATCTTGAATCAAGAAATCAGAAAATTGTCAAGACAGGTTATCAGAGTAAAATGGTCGCTGCATGCGAGAGAGGAGATTATATATGAGCTTCTTCAGCATCTACGAGGATATGTGACAAAAAGCTTATTGGAGAGCATAAGAAAGAGTACTAGACAAATGATTGAAGAGCAAGAAGCAGTTCGTGATCGTCTATTCACCATTCAAGATGTCATGCAGAGTAATGTTCGTGCATGGTTACAGGACAAAAGCCTCCGAGTAACTCATAACTTGGCGGTTTTTGGTGGCTGTGGCCTTGTTCTTTCCATTATAACTGGGCTGTTCGGAATCAACGTAGACGGGATCCCCGGGGCGCAGGATACACCATATGCATTTGGATTGTTTACAGCAATCCTATTCTTCTTAGGGATTGTACTGATGGGAGGGGGCGTGGTTTACCTCGGCTTGAAGAAGCCCATGGCAGAAGAGATGGTTGAATTCAGGAAGCTGGAACTGCAAGAGTTGGTTAAAATGTTTCAGCATGAGGCTGAAACTCATGCTCAGGTACGAAAATCTGTTACTCGCAATAATATACCTCCTACTGCAAGAGATATTTTCACAGCTGATGCGGATTATGTGCTCATCGAATGA

Protein sequence

MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDRQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHPSVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIFTADADYVLIE
Homology
BLAST of Lag0025064 vs. NCBI nr
Match: XP_038894745.1 (uncharacterized protein LOC120083181 isoform X1 [Benincasa hispida])

HSP 1 Score: 1031.9 bits (2667), Expect = 1.9e-297
Identity = 507/550 (92.18%), Postives = 527/550 (95.82%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           M+VHHSSH +SVEKPEA KPFNKDA LGCDLWTDGLICAFEYIGRPKKSIDSKS+SKM D
Sbjct: 1   MNVHHSSHRNSVEKPEAHKPFNKDAALGCDLWTDGLICAFEYIGRPKKSIDSKSKSKMTD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           R+QMNSEFPT  +PTTELNNGNA   ERK   EPTSS+DLKS CGQFCEREKVE++SWLP
Sbjct: 61  RRQMNSEFPTMLLPTTELNNGNANTHERKILFEPTSSEDLKSPCGQFCEREKVESNSWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSA+HP
Sbjct: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSANHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPFS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           DEDDIPIVLRSWQAQNFLITSLHVKG I+NIN+LGITEVQELLIIG YNAPRTVHEV+A 
Sbjct: 241 DEDDIPIVLRSWQAQNFLITSLHVKGSISNINILGITEVQELLIIGGYNAPRTVHEVVAH 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEI+YELLQHLRGYVTK LLESIRKSTRQMIEEQEAVR RLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIVYELLQHLRGYVTKGLLESIRKSTRQMIEEQEAVRGRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFG+FTAILFFLGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGIFTAILFFLGIAL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           M GG+VYLGLKKPMA++MVE RKLELQELVKMFQHEAETHAQVRKS+TRNN+PPTARDIF
Sbjct: 481 MAGGLVYLGLKKPMADQMVEVRKLELQELVKMFQHEAETHAQVRKSITRNNMPPTARDIF 540

Query: 541 TADADYVLIE 551
            ADADYVLIE
Sbjct: 541 PADADYVLIE 550

BLAST of Lag0025064 vs. NCBI nr
Match: XP_022955146.1 (uncharacterized protein LOC111457200 [Cucurbita moschata])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.5e-292
Identity = 497/550 (90.36%), Postives = 524/550 (95.27%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDV+HSS  ++VEKPEA KPFNKDA LG DLWTDGLICAFE+IGRPKKSIDSKSR+KMPD
Sbjct: 1   MDVNHSSRGNNVEKPEAHKPFNKDAALGYDLWTDGLICAFEFIGRPKKSIDSKSRTKMPD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           +QQ+NSEFPTTR PTTELNN N IARER+R LEPTS+DD KSQCGQF ERE+ E ++WLP
Sbjct: 61  KQQLNSEFPTTRTPTTELNNDNVIARERRRLLEPTSTDDPKSQCGQFYERERFEGNNWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWA MSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVDAEWALMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           +EDDIPIVLRSWQAQNFLITSLHVKGP++NINVLG+TEVQELLI G YNAPRTVHEVIAQ
Sbjct: 241 EEDDIPIVLRSWQAQNFLITSLHVKGPVSNINVLGVTEVQELLITGGYNAPRTVHEVIAQ 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           L CRL+RWDDRLFRK IFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LTCRLSRWDDRLFRKYIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEIIYELLQHLRGYV KSLLES+RKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIIYELLQHLRGYVAKSLLESVRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGA+DTPYAF LF+ ILF LGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAEDTPYAFALFSGILFLLGIGL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           MGGG+VYLGLKKPM +EMVE RK+ELQELVKMFQHEAETHAQVRKS+TRNN+PPTARD+F
Sbjct: 481 MGGGLVYLGLKKPMTDEMVEVRKMELQELVKMFQHEAETHAQVRKSITRNNMPPTARDLF 540

Query: 541 TADADYVLIE 551
           TADADYVLIE
Sbjct: 541 TADADYVLIE 550

BLAST of Lag0025064 vs. NCBI nr
Match: XP_022140184.1 (uncharacterized protein LOC111010914 isoform X1 [Momordica charantia])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.5e-292
Identity = 501/540 (92.78%), Postives = 517/540 (95.74%), Query Frame = 0

Query: 11  SVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDRQQMNSEFPT 70
           SVEKPEA KP +KDA LGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD QQMNSEFPT
Sbjct: 5   SVEKPEAHKPCHKDAALGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDTQQMNSEFPT 64

Query: 71  TRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELV 130
           TRIPTTELNNGN   R RKR LEPTSSDDLKSQCGQFCERE+ E++SWLPIGW RISELV
Sbjct: 65  TRIPTTELNNGNGNTRGRKRLLEPTSSDDLKSQCGQFCERERFESNSWLPIGWARISELV 124

Query: 131 QTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHPSVEAWLRSAH 190
           QTIQVDAEWASMSCD MDEEDDLTVADL APYWKRPAGPIWWCHVSA HPSVEAWLR+AH
Sbjct: 125 QTIQVDAEWASMSCDVMDEEDDLTVADLAAPYWKRPAGPIWWCHVSACHPSVEAWLRTAH 184

Query: 191 WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLR 250
           WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP SDEDDIP+VLR
Sbjct: 185 WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLSDEDDIPVVLR 244

Query: 251 SWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDD 310
           SWQAQNFLITSLHVKGPI+NIN LGITEVQELLIIG YN+PRTVHEVIA LACRLTRWDD
Sbjct: 245 SWQAQNFLITSLHVKGPISNINALGITEVQELLIIGGYNSPRTVHEVIAHLACRLTRWDD 304

Query: 311 RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIY 370
           RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEI+Y
Sbjct: 305 RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIVY 364

Query: 371 ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH 430
           ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH
Sbjct: 365 ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH 424

Query: 431 NLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGL 490
           NLAVFGGCGLVLSIITGLFGINVDGIPG+QDTPYAFGLF AILFFLGIVLM GG++YLGL
Sbjct: 425 NLAVFGGCGLVLSIITGLFGINVDGIPGSQDTPYAFGLFAAILFFLGIVLMVGGLIYLGL 484

Query: 491 KKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIFTADADYVLIE 550
           KKPMAE++VE RKLELQELVKMFQHEAETHAQVRKS TRNN+ PTARD+FTADADYVLIE
Sbjct: 485 KKPMAEDLVEVRKLELQELVKMFQHEAETHAQVRKSRTRNNMTPTARDVFTADADYVLIE 544

BLAST of Lag0025064 vs. NCBI nr
Match: KAG7012514.1 (hypothetical protein SDJN02_25266 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.3e-292
Identity = 496/550 (90.18%), Postives = 524/550 (95.27%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDV+HSS  ++VEKPEA KPFNKDA LG DLWTDGLICAFE+IGRPKKSIDSKSR+KMPD
Sbjct: 1   MDVNHSSRGNNVEKPEAHKPFNKDAALGYDLWTDGLICAFEFIGRPKKSIDSKSRTKMPD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           +QQ+NSEFPTTR PTTELNN N IARER+R LEPTS+DD KSQCGQF ERE+ E ++WLP
Sbjct: 61  KQQLNSEFPTTRTPTTELNNDNVIARERRRLLEPTSTDDPKSQCGQFYERERFEGNNWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWA MSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVDAEWALMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           +EDDIPIVLRSWQAQNFLITSLHVKGP++NINVLG+TEVQELLI G Y+APRTVHEVIAQ
Sbjct: 241 EEDDIPIVLRSWQAQNFLITSLHVKGPVSNINVLGVTEVQELLITGGYSAPRTVHEVIAQ 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           L CRL+RWDDRLFRK IFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LTCRLSRWDDRLFRKYIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEIIYELLQHLRGYV KSLLES+RKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIIYELLQHLRGYVAKSLLESVRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGA+DTPYAF LF+ ILF LGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAEDTPYAFALFSGILFLLGIGL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           MGGG+VYLGLKKPM +EMVE RK+ELQELVKMFQHEAETHAQVRKS+TRNN+PPTARD+F
Sbjct: 481 MGGGLVYLGLKKPMTDEMVEVRKMELQELVKMFQHEAETHAQVRKSITRNNMPPTARDLF 540

Query: 541 TADADYVLIE 551
           TADADYVLIE
Sbjct: 541 TADADYVLIE 550

BLAST of Lag0025064 vs. NCBI nr
Match: XP_023542326.1 (uncharacterized protein LOC111802258 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.2e-291
Identity = 495/550 (90.00%), Postives = 524/550 (95.27%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDV+HSS  ++VEKPEA KPFNKDA LG DLWTDGLICAFE+IGRPKKSIDSKSR+KMPD
Sbjct: 1   MDVNHSSRGNNVEKPEAHKPFNKDAALGYDLWTDGLICAFEFIGRPKKSIDSKSRTKMPD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           +QQ+NSEFPTTR PTTELNN N IARER+R LEPTS+D+ KSQCGQF ERE+ E ++WLP
Sbjct: 61  KQQLNSEFPTTRTPTTELNNDNVIARERRRLLEPTSTDEPKSQCGQFYERERFEGNNWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWA MSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVDAEWALMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           +EDDIPIVLRSWQAQNFLITSLHVKGP++NINVLG+TEVQELLI G YNAPRTVHEVIAQ
Sbjct: 241 EEDDIPIVLRSWQAQNFLITSLHVKGPVSNINVLGVTEVQELLITGGYNAPRTVHEVIAQ 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           L CRL+RWDDRLFRK IFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LTCRLSRWDDRLFRKYIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEIIYELLQHLRGYV KSLLES+RKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIIYELLQHLRGYVAKSLLESVRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGA+DTPYAF LF+ +LF LGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAEDTPYAFALFSGLLFLLGIGL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           MGGG+VYLGLKKPM +EMVE RK+ELQELVKMFQHEAETHAQVRKS+TRNN+PPTARD+F
Sbjct: 481 MGGGLVYLGLKKPMTDEMVEVRKMELQELVKMFQHEAETHAQVRKSITRNNMPPTARDLF 540

Query: 541 TADADYVLIE 551
           TADADYVLIE
Sbjct: 541 TADADYVLIE 550

BLAST of Lag0025064 vs. ExPASy TrEMBL
Match: A0A6J1CF12 (uncharacterized protein LOC111010914 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010914 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.2e-292
Identity = 501/540 (92.78%), Postives = 517/540 (95.74%), Query Frame = 0

Query: 11  SVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDRQQMNSEFPT 70
           SVEKPEA KP +KDA LGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD QQMNSEFPT
Sbjct: 5   SVEKPEAHKPCHKDAALGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDTQQMNSEFPT 64

Query: 71  TRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELV 130
           TRIPTTELNNGN   R RKR LEPTSSDDLKSQCGQFCERE+ E++SWLPIGW RISELV
Sbjct: 65  TRIPTTELNNGNGNTRGRKRLLEPTSSDDLKSQCGQFCERERFESNSWLPIGWARISELV 124

Query: 131 QTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHPSVEAWLRSAH 190
           QTIQVDAEWASMSCD MDEEDDLTVADL APYWKRPAGPIWWCHVSA HPSVEAWLR+AH
Sbjct: 125 QTIQVDAEWASMSCDVMDEEDDLTVADLAAPYWKRPAGPIWWCHVSACHPSVEAWLRTAH 184

Query: 191 WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLR 250
           WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP SDEDDIP+VLR
Sbjct: 185 WLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLSDEDDIPVVLR 244

Query: 251 SWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDD 310
           SWQAQNFLITSLHVKGPI+NIN LGITEVQELLIIG YN+PRTVHEVIA LACRLTRWDD
Sbjct: 245 SWQAQNFLITSLHVKGPISNINALGITEVQELLIIGGYNSPRTVHEVIAHLACRLTRWDD 304

Query: 311 RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIY 370
           RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEI+Y
Sbjct: 305 RLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIVY 364

Query: 371 ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH 430
           ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH
Sbjct: 365 ELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTH 424

Query: 431 NLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGL 490
           NLAVFGGCGLVLSIITGLFGINVDGIPG+QDTPYAFGLF AILFFLGIVLM GG++YLGL
Sbjct: 425 NLAVFGGCGLVLSIITGLFGINVDGIPGSQDTPYAFGLFAAILFFLGIVLMVGGLIYLGL 484

Query: 491 KKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIFTADADYVLIE 550
           KKPMAE++VE RKLELQELVKMFQHEAETHAQVRKS TRNN+ PTARD+FTADADYVLIE
Sbjct: 485 KKPMAEDLVEVRKLELQELVKMFQHEAETHAQVRKSRTRNNMTPTARDVFTADADYVLIE 544

BLAST of Lag0025064 vs. ExPASy TrEMBL
Match: A0A6J1GSY0 (uncharacterized protein LOC111457200 OS=Cucurbita moschata OX=3662 GN=LOC111457200 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.2e-292
Identity = 497/550 (90.36%), Postives = 524/550 (95.27%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDV+HSS  ++VEKPEA KPFNKDA LG DLWTDGLICAFE+IGRPKKSIDSKSR+KMPD
Sbjct: 1   MDVNHSSRGNNVEKPEAHKPFNKDAALGYDLWTDGLICAFEFIGRPKKSIDSKSRTKMPD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           +QQ+NSEFPTTR PTTELNN N IARER+R LEPTS+DD KSQCGQF ERE+ E ++WLP
Sbjct: 61  KQQLNSEFPTTRTPTTELNNDNVIARERRRLLEPTSTDDPKSQCGQFYERERFEGNNWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWA MSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVDAEWALMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           +EDDIPIVLRSWQAQNFLITSLHVKGP++NINVLG+TEVQELLI G YNAPRTVHEVIAQ
Sbjct: 241 EEDDIPIVLRSWQAQNFLITSLHVKGPVSNINVLGVTEVQELLITGGYNAPRTVHEVIAQ 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           L CRL+RWDDRLFRK IFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LTCRLSRWDDRLFRKYIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEIIYELLQHLRGYV KSLLES+RKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIIYELLQHLRGYVAKSLLESVRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGA+DTPYAF LF+ ILF LGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAEDTPYAFALFSGILFLLGIGL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           MGGG+VYLGLKKPM +EMVE RK+ELQELVKMFQHEAETHAQVRKS+TRNN+PPTARD+F
Sbjct: 481 MGGGLVYLGLKKPMTDEMVEVRKMELQELVKMFQHEAETHAQVRKSITRNNMPPTARDLF 540

Query: 541 TADADYVLIE 551
           TADADYVLIE
Sbjct: 541 TADADYVLIE 550

BLAST of Lag0025064 vs. ExPASy TrEMBL
Match: A0A6J1K2A4 (uncharacterized protein LOC111490402 OS=Cucurbita maxima OX=3661 GN=LOC111490402 PE=4 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.7e-291
Identity = 495/550 (90.00%), Postives = 523/550 (95.09%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDV+HSS  ++VEKPEA KPFNKDA LG DLWTDGLICAFE+IGRPKKSIDSKSR+KMPD
Sbjct: 1   MDVNHSSRGNNVEKPEAHKPFNKDAALGYDLWTDGLICAFEFIGRPKKSIDSKSRTKMPD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           ++Q+NSEFPTTR PTTELN  N IARER+R LEPTS+DD KSQCGQF ERE+ E ++WLP
Sbjct: 61  KKQLNSEFPTTRSPTTELNYDNVIARERRRLLEPTSTDDPKSQCGQFYERERFEGNNWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQVDAEWA MSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVDAEWALMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPLS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           +EDDIPIVLRSWQAQNFLITSLHVKGP++NINVLG+TEVQELLI G YNAPRTVHEVIAQ
Sbjct: 241 EEDDIPIVLRSWQAQNFLITSLHVKGPVSNINVLGVTEVQELLITGGYNAPRTVHEVIAQ 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           L CRL+RWDDRLFRK IFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LTCRLSRWDDRLFRKYIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEIIYELLQHLRGYV KSLLES+RKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIIYELLQHLRGYVAKSLLESVRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGA+DTPYAF LF+ ILF LGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAEDTPYAFALFSGILFLLGIAL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           MGGG+VYLGLKKPM +EMVE RK+ELQELVKMFQHEAETHAQVRKS+TRNN+PPTARD+F
Sbjct: 481 MGGGLVYLGLKKPMTDEMVEVRKMELQELVKMFQHEAETHAQVRKSITRNNMPPTARDLF 540

Query: 541 TADADYVLIE 551
           TADADYVLIE
Sbjct: 541 TADADYVLIE 550

BLAST of Lag0025064 vs. ExPASy TrEMBL
Match: A0A1S3B997 (uncharacterized protein LOC103487421 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487421 PE=4 SV=1)

HSP 1 Score: 1005.7 bits (2599), Expect = 7.2e-290
Identity = 496/550 (90.18%), Postives = 521/550 (94.73%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDVHHS H +SVEKPEA KPFNKDAT G DLWTDGLICAFEYIGRPKK+ DSKS+SKM D
Sbjct: 1   MDVHHSPHRNSVEKPEAHKPFNKDATPGYDLWTDGLICAFEYIGRPKKATDSKSKSKMSD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           R+QMNSEFPT    T ELNNGN    ERKR  EPTS +D+++QCGQFCEREKVE++SWLP
Sbjct: 61  RRQMNSEFPT----TPELNNGNVNTHERKRSFEPTSPEDVRTQCGQFCEREKVESNSWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQV+AEWASMS DFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVEAEWASMSYDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPFS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           DEDDIP+VLRSWQAQNFLITSLHVKGPI+NIN+LGITEVQELLIIG YNAPRTVHEVIA 
Sbjct: 241 DEDDIPVVLRSWQAQNFLITSLHVKGPISNINILGITEVQELLIIGGYNAPRTVHEVIAH 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEI+YELLQHLRGYVTK LLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEIVYELLQHLRGYVTKGLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQ+TPYAFG+FTAILFFLGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQNTPYAFGMFTAILFFLGIAL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           M GG+VYLGLKKPM ++MVE RKLEL+ELVKMFQHEAETHAQVRK++TRNN+PPTARDIF
Sbjct: 481 MAGGLVYLGLKKPMPDQMVEVRKLELEELVKMFQHEAETHAQVRKTLTRNNMPPTARDIF 540

Query: 541 TADADYVLIE 551
            ADADYVLIE
Sbjct: 541 PADADYVLIE 546

BLAST of Lag0025064 vs. ExPASy TrEMBL
Match: A0A0A0LX35 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G453990 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 495/550 (90.00%), Postives = 518/550 (94.18%), Query Frame = 0

Query: 1   MDVHHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPD 60
           MDVHHS H +SVEKPEA KPFNKDAT G DLWTDGLICAFEYIG+P+KS DSKS+SKM D
Sbjct: 1   MDVHHSPHRNSVEKPEAHKPFNKDATPGYDLWTDGLICAFEYIGKPRKSTDSKSKSKMSD 60

Query: 61  RQQMNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLP 120
           R Q NSEFPTT   TTELN+GNA   ERKR  EPTS +DL++ CGQF EREKVE++SWLP
Sbjct: 61  RWQTNSEFPTT---TTELNSGNANTHERKRSFEPTSPEDLRTHCGQFSEREKVESNSWLP 120

Query: 121 IGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180
           IGWDRISELVQTIQV+AEWASMS DFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP
Sbjct: 121 IGWDRISELVQTIQVEAEWASMSYDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHP 180

Query: 181 SVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSS 240
           SVEAWLR+AHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDP S
Sbjct: 181 SVEAWLRTAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPFS 240

Query: 241 DEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQ 300
           DEDDIP+VLRSWQAQNFLITSLHVKGPI+N N+LGITEVQELLIIG YNAPRTVHEVIA 
Sbjct: 241 DEDDIPVVLRSWQAQNFLITSLHVKGPISNPNILGITEVQELLIIGGYNAPRTVHEVIAH 300

Query: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360
           LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW
Sbjct: 301 LACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKW 360

Query: 361 SLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420
           SLHAREEI+YELLQHLRGYVTK LLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW
Sbjct: 361 SLHAREEILYELLQHLRGYVTKGLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAW 420

Query: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVL 480
           LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQ+TPYAFG+FTAILFFLGI L
Sbjct: 421 LQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQNTPYAFGMFTAILFFLGIAL 480

Query: 481 MGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIF 540
           M GG+VYLGLKKPM ++MVE RKLEL+ELVKMFQHEAETHAQVRKS+TR NIPPTARDIF
Sbjct: 481 MAGGLVYLGLKKPMPDQMVEVRKLELEELVKMFQHEAETHAQVRKSLTRKNIPPTARDIF 540

Query: 541 TADADYVLIE 551
            ADADYVLIE
Sbjct: 541 PADADYVLIE 547

BLAST of Lag0025064 vs. TAIR 10
Match: AT1G29820.1 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 699.9 bits (1805), Expect = 1.6e-201
Identity = 358/561 (63.81%), Postives = 431/561 (76.83%), Query Frame = 0

Query: 4   HHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDRQQ 63
           +HS   +S  K    K  N       DLWTDG+ICAFE+I  PKK +DSK   K   +Q+
Sbjct: 7   NHSVSQESKSKKLESK--NHSGVPKSDLWTDGIICAFEFIRGPKKHVDSKQLEKGALKQE 66

Query: 64  ---MNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCE----------- 123
              ++  FP                     F++ +  DDL+S+     +           
Sbjct: 67  DGPISHSFP-----------------GHNPFVDSSPVDDLRSRSVSSLDFKEAHLLPSGH 126

Query: 124 REKVENHSWLPIGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGP 183
            E+ E   W+PIGW RI+ELVQ +QV+AEW ++  + +D+E+D+ V DL APYW+RP GP
Sbjct: 127 VERYEGSHWVPIGWARITELVQMVQVNAEWPNL--ELIDDEEDVPVTDLAAPYWERPGGP 186

Query: 184 IWWCHVSASHPSVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFE 243
            WWCH+SA +  VE WLRSA WLHPAISLALRDES+LIS+RM+HL YEVPVRVAGGLLFE
Sbjct: 187 TWWCHLSAGNSFVEGWLRSATWLHPAISLALRDESKLISERMRHLLYEVPVRVAGGLLFE 246

Query: 244 LLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYN 303
           LLGQS GDP   EDD+P+V RSWQA+NFL++ +H+KG +TN NVLGITEV+ELL  G YN
Sbjct: 247 LLGQSVGDPVISEDDVPVVFRSWQAKNFLVSVMHIKGNVTNTNVLGITEVEELLYAGGYN 306

Query: 304 APRTVHEVIAQLACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIR 363
            PRTVHEVIA LACRL+RWDDRLFRKSIFGAADEIELKFM+RRN+ED++LFSIILNQEIR
Sbjct: 307 VPRTVHEVIAHLACRLSRWDDRLFRKSIFGAADEIELKFMNRRNYEDLNLFSIILNQEIR 366

Query: 364 KLSRQVIRVKWSLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTI 423
           KLSRQVIRVKWSLHAREEII+ELLQHLRG + + LL+ +RK+TR+M+EEQEAVR RLFTI
Sbjct: 367 KLSRQVIRVKWSLHAREEIIFELLQHLRGNIARHLLDGLRKNTREMLEEQEAVRGRLFTI 426

Query: 424 QDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLF 483
           QDVMQS+VRAWLQDKSLRV+HNLAVFGGCGLVL+II GLFGINVDGIPGAQ+TPYAFGLF
Sbjct: 427 QDVMQSSVRAWLQDKSLRVSHNLAVFGGCGLVLTIIVGLFGINVDGIPGAQNTPYAFGLF 486

Query: 484 TAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTR 543
           T ++  LG +L+  G+VYLGLKKP+ EE VE RKLELQ++VK+FQHEAETHAQ+R    R
Sbjct: 487 TFLMVLLGAILIVVGLVYLGLKKPITEEQVEVRKLELQDVVKIFQHEAETHAQLR----R 540

Query: 544 NNIPPTARDIFTADADYVLIE 551
           NN+ PTA D+F  DADY+LI+
Sbjct: 547 NNLSPTAGDVF--DADYILIQ 540

BLAST of Lag0025064 vs. TAIR 10
Match: AT1G29820.2 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 699.9 bits (1805), Expect = 1.6e-201
Identity = 358/561 (63.81%), Postives = 431/561 (76.83%), Query Frame = 0

Query: 4   HHSSHADSVEKPEAPKPFNKDATLGCDLWTDGLICAFEYIGRPKKSIDSKSRSKMPDRQQ 63
           +HS   +S  K    K  N       DLWTDG+ICAFE+I  PKK +DSK   K   +Q+
Sbjct: 7   NHSVSQESKSKKLESK--NHSGVPKSDLWTDGIICAFEFIRGPKKHVDSKQLEKGALKQE 66

Query: 64  ---MNSEFPTTRIPTTELNNGNAIARERKRFLEPTSSDDLKSQCGQFCE----------- 123
              ++  FP                     F++ +  DDL+S+     +           
Sbjct: 67  DGPISHSFP-----------------GHNPFVDSSPVDDLRSRSVSSLDFKEAHLLPSGH 126

Query: 124 REKVENHSWLPIGWDRISELVQTIQVDAEWASMSCDFMDEEDDLTVADLVAPYWKRPAGP 183
            E+ E   W+PIGW RI+ELVQ +QV+AEW ++  + +D+E+D+ V DL APYW+RP GP
Sbjct: 127 VERYEGSHWVPIGWARITELVQMVQVNAEWPNL--ELIDDEEDVPVTDLAAPYWERPGGP 186

Query: 184 IWWCHVSASHPSVEAWLRSAHWLHPAISLALRDESRLISDRMKHLFYEVPVRVAGGLLFE 243
            WWCH+SA +  VE WLRSA WLHPAISLALRDES+LIS+RM+HL YEVPVRVAGGLLFE
Sbjct: 187 TWWCHLSAGNSFVEGWLRSATWLHPAISLALRDESKLISERMRHLLYEVPVRVAGGLLFE 246

Query: 244 LLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSLHVKGPITNINVLGITEVQELLIIGDYN 303
           LLGQS GDP   EDD+P+V RSWQA+NFL++ +H+KG +TN NVLGITEV+ELL  G YN
Sbjct: 247 LLGQSVGDPVISEDDVPVVFRSWQAKNFLVSVMHIKGNVTNTNVLGITEVEELLYAGGYN 306

Query: 304 APRTVHEVIAQLACRLTRWDDRLFRKSIFGAADEIELKFMDRRNHEDMHLFSIILNQEIR 363
            PRTVHEVIA LACRL+RWDDRLFRKSIFGAADEIELKFM+RRN+ED++LFSIILNQEIR
Sbjct: 307 VPRTVHEVIAHLACRLSRWDDRLFRKSIFGAADEIELKFMNRRNYEDLNLFSIILNQEIR 366

Query: 364 KLSRQVIRVKWSLHAREEIIYELLQHLRGYVTKSLLESIRKSTRQMIEEQEAVRDRLFTI 423
           KLSRQVIRVKWSLHAREEII+ELLQHLRG + + LL+ +RK+TR+M+EEQEAVR RLFTI
Sbjct: 367 KLSRQVIRVKWSLHAREEIIFELLQHLRGNIARHLLDGLRKNTREMLEEQEAVRGRLFTI 426

Query: 424 QDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVLSIITGLFGINVDGIPGAQDTPYAFGLF 483
           QDVMQS+VRAWLQDKSLRV+HNLAVFGGCGLVL+II GLFGINVDGIPGAQ+TPYAFGLF
Sbjct: 427 QDVMQSSVRAWLQDKSLRVSHNLAVFGGCGLVLTIIVGLFGINVDGIPGAQNTPYAFGLF 486

Query: 484 TAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFRKLELQELVKMFQHEAETHAQVRKSVTR 543
           T ++  LG +L+  G+VYLGLKKP+ EE VE RKLELQ++VK+FQHEAETHAQ+R    R
Sbjct: 487 TFLMVLLGAILIVVGLVYLGLKKPITEEQVEVRKLELQDVVKIFQHEAETHAQLR----R 540

Query: 544 NNIPPTARDIFTADADYVLIE 551
           NN+ PTA D+F  DADY+LI+
Sbjct: 547 NNLSPTAGDVF--DADYILIQ 540

BLAST of Lag0025064 vs. TAIR 10
Match: AT1G29830.1 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-187
Identity = 328/528 (62.12%), Postives = 400/528 (75.76%), Query Frame = 0

Query: 30  DLWTDGLICAFEYIGRPKKSIDSKSRSK-------MPDRQQMNSEFPTTRIPTTELNNGN 89
           DLWTDG+ICAFE+I  PKK +DSK   K        P R       P    P  +     
Sbjct: 24  DLWTDGIICAFEFIRGPKKHVDSKQLYKGYLKQEDGPIRHSFPGHNPFIDSPAVDYLRSR 83

Query: 90  AIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELVQTIQVDAEWASM 149
           ++          +S D  ++        E+ E   W+PIGW RI++LVQ +QV+AEW ++
Sbjct: 84  SV----------SSLDFKEAHVLPSGHVERSEGSHWVPIGWTRITKLVQQVQVNAEWPNL 143

Query: 150 SCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHPSVEAWLRSAHWLHPAISLALRD 209
               +D+E+D+ V DL APYW+RP GP WWCH++A +  VE WLRSA WLHPAISLALRD
Sbjct: 144 K--LIDDEEDVPVTDLAAPYWERPGGPTWWCHLTAGNSFVEGWLRSATWLHPAISLALRD 203

Query: 210 ESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSL 269
           ES+LIS+RM+HL YEVPVRV GGLLFELLGQS GDP   EDD+P+V RSWQA+NFL++ +
Sbjct: 204 ESKLISERMRHLLYEVPVRVDGGLLFELLGQSMGDPVIGEDDVPVVFRSWQAKNFLVSVM 263

Query: 270 HVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDDRLFRKSIFGAAD 329
           H+KG ++  NVLGITEV+ELL  G YN PRT+HEVIA LACRL+RWDDRLFRKSIFGAAD
Sbjct: 264 HIKGNVSKSNVLGITEVEELLYAGSYNVPRTIHEVIAHLACRLSRWDDRLFRKSIFGAAD 323

Query: 330 EIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIYELLQHLRGYVTK 389
           EIELKFM+RRNHED++LFSIILNQEIRKL+RQ IRVKWSLHAREEII ELLQHLRG + +
Sbjct: 324 EIELKFMNRRNHEDLNLFSIILNQEIRKLARQTIRVKWSLHAREEIILELLQHLRGNIPR 383

Query: 390 SLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVL 449
            LLE +R +TR+M+EEQEAVR RLFTIQD +QSN+R+WLQD+SL  +HNLA+FGGCGLVL
Sbjct: 384 HLLEGLRNNTREMLEEQEAVRGRLFTIQDNIQSNIRSWLQDQSLNGSHNLAIFGGCGLVL 443

Query: 450 SIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFR 509
           +II GLF +N+DG+PG + TPYAF LF+  L  +GIVL+  G+ YLG KKP+ EE VE R
Sbjct: 444 TIILGLFSVNLDGVPGVKHTPYAFVLFSVFLVLIGIVLIAFGLRYLGPKKPITEEHVEAR 503

Query: 510 KLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIFTADADYVLIE 551
           KLELQ +VK+FQHEAETHAQ+R    RNN+ PTA D+F  DADY LI+
Sbjct: 504 KLELQNVVKIFQHEAETHAQLR----RNNLSPTAGDVF--DADYFLIQ 533

BLAST of Lag0025064 vs. TAIR 10
Match: AT1G29830.3 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-187
Identity = 328/528 (62.12%), Postives = 400/528 (75.76%), Query Frame = 0

Query: 30  DLWTDGLICAFEYIGRPKKSIDSKSRSK-------MPDRQQMNSEFPTTRIPTTELNNGN 89
           DLWTDG+ICAFE+I  PKK +DSK   K        P R       P    P  +     
Sbjct: 59  DLWTDGIICAFEFIRGPKKHVDSKQLYKGYLKQEDGPIRHSFPGHNPFIDSPAVDYLRSR 118

Query: 90  AIARERKRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELVQTIQVDAEWASM 149
           ++          +S D  ++        E+ E   W+PIGW RI++LVQ +QV+AEW ++
Sbjct: 119 SV----------SSLDFKEAHVLPSGHVERSEGSHWVPIGWTRITKLVQQVQVNAEWPNL 178

Query: 150 SCDFMDEEDDLTVADLVAPYWKRPAGPIWWCHVSASHPSVEAWLRSAHWLHPAISLALRD 209
               +D+E+D+ V DL APYW+RP GP WWCH++A +  VE WLRSA WLHPAISLALRD
Sbjct: 179 K--LIDDEEDVPVTDLAAPYWERPGGPTWWCHLTAGNSFVEGWLRSATWLHPAISLALRD 238

Query: 210 ESRLISDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSL 269
           ES+LIS+RM+HL YEVPVRV GGLLFELLGQS GDP   EDD+P+V RSWQA+NFL++ +
Sbjct: 239 ESKLISERMRHLLYEVPVRVDGGLLFELLGQSMGDPVIGEDDVPVVFRSWQAKNFLVSVM 298

Query: 270 HVKGPITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDDRLFRKSIFGAAD 329
           H+KG ++  NVLGITEV+ELL  G YN PRT+HEVIA LACRL+RWDDRLFRKSIFGAAD
Sbjct: 299 HIKGNVSKSNVLGITEVEELLYAGSYNVPRTIHEVIAHLACRLSRWDDRLFRKSIFGAAD 358

Query: 330 EIELKFMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIYELLQHLRGYVTK 389
           EIELKFM+RRNHED++LFSIILNQEIRKL+RQ IRVKWSLHAREEII ELLQHLRG + +
Sbjct: 359 EIELKFMNRRNHEDLNLFSIILNQEIRKLARQTIRVKWSLHAREEIILELLQHLRGNIPR 418

Query: 390 SLLESIRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVL 449
            LLE +R +TR+M+EEQEAVR RLFTIQD +QSN+R+WLQD+SL  +HNLA+FGGCGLVL
Sbjct: 419 HLLEGLRNNTREMLEEQEAVRGRLFTIQDNIQSNIRSWLQDQSLNGSHNLAIFGGCGLVL 478

Query: 450 SIITGLFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFR 509
           +II GLF +N+DG+PG + TPYAF LF+  L  +GIVL+  G+ YLG KKP+ EE VE R
Sbjct: 479 TIILGLFSVNLDGVPGVKHTPYAFVLFSVFLVLIGIVLIAFGLRYLGPKKPITEEHVEAR 538

Query: 510 KLELQELVKMFQHEAETHAQVRKSVTRNNIPPTARDIFTADADYVLIE 551
           KLELQ +VK+FQHEAETHAQ+R    RNN+ PTA D+F  DADY LI+
Sbjct: 539 KLELQNVVKIFQHEAETHAQLR----RNNLSPTAGDVF--DADYFLIQ 568

BLAST of Lag0025064 vs. TAIR 10
Match: AT2G42950.1 (Magnesium transporter CorA-like family protein )

HSP 1 Score: 613.6 bits (1581), Expect = 1.5e-175
Identity = 324/525 (61.71%), Postives = 384/525 (73.14%), Query Frame = 0

Query: 30  DLWTDGLICAFEYI-GRPKKSIDSKSRSKMPDRQQMNSEFPTTRIPTTELNNGNAIARER 89
           +LWT+GLICAFE+  GR K +      S +  ++Q           T +   G       
Sbjct: 14  NLWTNGLICAFEFCQGRRKNNTSVHGDSSLRIKKQ--------ECETDQFGTG------- 73

Query: 90  KRFLEPTSSDDLKSQCGQFCEREKVENHSWLPIGWDRISELVQTIQVDAEWASMSCDFMD 149
                               E E+     W  IGWDRISELVQT+QVD  W     D   
Sbjct: 74  --------------------EDEEHSRSYWRGIGWDRISELVQTVQVDNNWDLRKIDL-- 133

Query: 150 EEDDLTVADLVAPYWKRP-AGPIWWCHVSASHPSVEAWLRSAHWLHPAISLALRDESRLI 209
           +ED+ TVA+L APYW+RP AGP WWCH+ A+H  + +WLR+AHWLHPA+SLALRDES+LI
Sbjct: 134 DEDEATVAELAAPYWERPLAGPTWWCHLDATHHGIASWLRNAHWLHPAVSLALRDESKLI 193

Query: 210 SDRMKHLFYEVPVRVAGGLLFELLGQSAGDPSSDEDDIPIVLRSWQAQNFLITSLHVKGP 269
           S+RMKH+FYEVPVRVAGGLLFELLGQSAGDP   EDDIPIVLRSWQAQNFL+T+LHVKG 
Sbjct: 194 SERMKHIFYEVPVRVAGGLLFELLGQSAGDPFIQEDDIPIVLRSWQAQNFLVTALHVKGF 253

Query: 270 ITNINVLGITEVQELLIIGDYNAPRTVHEVIAQLACRLTRWDDRLFRKSIFGAADEIELK 329
             NI+VLGITEVQE+LI G    PRTVHE+IA LACRL RWDDRLFRK IFGAADE+EL 
Sbjct: 254 ALNISVLGITEVQEMLIAGGACIPRTVHELIAHLACRLARWDDRLFRKYIFGAADEVELM 313

Query: 330 FMDRRNHEDMHLFSIILNQEIRKLSRQVIRVKWSLHAREEIIYELLQHLRGYVTKSLLES 389
           FM++R +ED +LF+ ILNQEIR+LS QVIRVKWSLHAREEI++ELLQ L+G  TK LLE 
Sbjct: 314 FMNKRLYEDPNLFTTILNQEIRRLSTQVIRVKWSLHAREEIVFELLQQLKGNRTKDLLEG 373

Query: 390 IRKSTRQMIEEQEAVRDRLFTIQDVMQSNVRAWLQDKSLRVTHNLAVFGGCGLVLSIITG 449
           I+KSTR MI EQEAVR RLFTIQDVMQ+ VRAWLQD+SL VTHNL +FGG GL+++I+TG
Sbjct: 374 IKKSTRDMINEQEAVRGRLFTIQDVMQNTVRAWLQDQSLTVTHNLGIFGGVGLLITIVTG 433

Query: 450 LFGINVDGIPGAQDTPYAFGLFTAILFFLGIVLMGGGVVYLGLKKPMAEEMVEFRKLELQ 509
           LFGINVDGIPGA+D P AF LF+ +LF  G+VL+  G++YLGLK+P+AEE VE RKLEL 
Sbjct: 434 LFGINVDGIPGAKDFPQAFALFSVVLFVSGLVLVVAGLIYLGLKEPVAEENVETRKLELD 493

Query: 510 ELVKMFQHEAETHAQVRKSVTRNNIPPTARD--IFTADADYVLIE 551
           E+VK FQ EAE+HAQV K V +N    T+R   I      YVL+E
Sbjct: 494 EMVKKFQQEAESHAQVCKKVPQNIERTTSRSRMIVHDPNGYVLLE 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894745.11.9e-29792.18uncharacterized protein LOC120083181 isoform X1 [Benincasa hispida][more]
XP_022955146.12.5e-29290.36uncharacterized protein LOC111457200 [Cucurbita moschata][more]
XP_022140184.12.5e-29292.78uncharacterized protein LOC111010914 isoform X1 [Momordica charantia][more]
KAG7012514.19.3e-29290.18hypothetical protein SDJN02_25266 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023542326.11.2e-29190.00uncharacterized protein LOC111802258 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CF121.2e-29292.78uncharacterized protein LOC111010914 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1GSY01.2e-29290.36uncharacterized protein LOC111457200 OS=Cucurbita moschata OX=3662 GN=LOC1114572... [more]
A0A6J1K2A41.7e-29190.00uncharacterized protein LOC111490402 OS=Cucurbita maxima OX=3661 GN=LOC111490402... [more]
A0A1S3B9977.2e-29090.18uncharacterized protein LOC103487421 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LX352.0e-28790.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G453990 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G29820.11.6e-20163.81Magnesium transporter CorA-like family protein [more]
AT1G29820.21.6e-20163.81Magnesium transporter CorA-like family protein [more]
AT1G29830.12.3e-18762.12Magnesium transporter CorA-like family protein [more]
AT1G29830.32.3e-18762.12Magnesium transporter CorA-like family protein [more]
AT2G42950.11.5e-17561.71Magnesium transporter CorA-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 418..486
e-value: 4.6E-6
score: 28.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR46950:SF2MAGNESIUM TRANSPORTER CORA-LIKE FAMILY PROTEINcoord: 5..550
NoneNo IPR availablePANTHERPTHR46950MAGNESIUM TRANSPORTER CORA-LIKE FAMILY PROTEINcoord: 5..550
NoneNo IPR availableSUPERFAMILY143865CorA soluble domain-likecoord: 155..417
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 429..480
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 346..480
e-value: 1.2E-11
score: 44.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0025064.1Lag0025064.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0015693 magnesium ion transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0046873 metal ion transmembrane transporter activity