Lag0025019 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0025019
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr10: 7803488 .. 7804050 (+)
RNA-Seq ExpressionLag0025019
SyntenyLag0025019
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGTGGATAAATTTGAAGAGTTTGTGGTGATGAGTCGGTGGGTATGGAGCAGGAGGAATAAGGAGCTATTTGAGGAAGTTGTTATGGGCGAGAAAGGGGACAGTGGGTGGGGGTGGATGGAAGATTATTTATCCCATTTTAGAGCTTTTAATCGCAGGAATTGTGGATCTGTAGTGAGGGGGTGGAAGCTGTGTGTTGGATTCCTCCTGAATTTCCTAATTATAAGCTAAATACAGATATTGCAGTGAATAGGGATCTCAATCTCAGCAATATTGGGGCTATTGTCATAAATGAGAAAGGGGAAGTGATGCTTACCTTGATGAGATTGGTGGAGTTTGTGCTTGATGTGGATGTGTTGGAAGCAATGGCAATTCGCGAAGGGCTGGCAGCTGTAGTGGATGCCGGCTTCTCGCGGGTGGTGGTGGAGACGGACTCGTCAAGCGTGGTGAAGCAGTTTCAGTCCGACGGGTACAATCTTTCGAAGATGGGCTTTCTGGTGGAGGAAATAAGAGGCATCTCTCGGGAGTTGAAGTTCTGTGAGGTCGGGTGGTGCAGTTGA

mRNA sequence

ATGTCAGTGGATAAATTTGAAGAGTTTGTGGTGATGAGTCGGTGGGTATGGAGCAGGAGGAATAAGGAGCTATTTGAGGAAGTTGTTATGGGCGAGAAAGGGGACAGTGGTGAGGGGGTGGAAGCTGTGTGTTGGATTCCTCCTGAATTTCCTAATTATAAGCTAAATACAGATATTGCAGTGAATAGGGATCTCAATCTCAGCAATATTGGGGCTATTGTCATAAATGAGAAAGGGGAAGTGATGCTTACCTTGATGAGATTGGTGGAGTTTGTGCTTGATGTGGATGTGTTGGAAGCAATGGCAATTCGCGAAGGGCTGGCAGCTGTAGTGGATGCCGGCTTCTCGCGGGTGGTGGTGGAGACGGACTCGTCAAGCGTGGTGAAGCAGTTTCAGTCCGACGGGTACAATCTTTCGAAGATGGGCTTTCTGGTGGAGGAAATAAGAGGCATCTCTCGGGAGTTGAAGTTCTGTGAGGTCGGGTGGTGCAGTTGA

Coding sequence (CDS)

ATGTCAGTGGATAAATTTGAAGAGTTTGTGGTGATGAGTCGGTGGGTATGGAGCAGGAGGAATAAGGAGCTATTTGAGGAAGTTGTTATGGGCGAGAAAGGGGACAGTGGTGAGGGGGTGGAAGCTGTGTGTTGGATTCCTCCTGAATTTCCTAATTATAAGCTAAATACAGATATTGCAGTGAATAGGGATCTCAATCTCAGCAATATTGGGGCTATTGTCATAAATGAGAAAGGGGAAGTGATGCTTACCTTGATGAGATTGGTGGAGTTTGTGCTTGATGTGGATGTGTTGGAAGCAATGGCAATTCGCGAAGGGCTGGCAGCTGTAGTGGATGCCGGCTTCTCGCGGGTGGTGGTGGAGACGGACTCGTCAAGCGTGGTGAAGCAGTTTCAGTCCGACGGGTACAATCTTTCGAAGATGGGCTTTCTGGTGGAGGAAATAAGAGGCATCTCTCGGGAGTTGAAGTTCTGTGAGGTCGGGTGGTGCAGTTGA

Protein sequence

MSVDKFEEFVVMSRWVWSRRNKELFEEVVMGEKGDSGEGVEAVCWIPPEFPNYKLNTDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEAMAIREGLAAVVDAGFSRVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELKFCEVGWCS
Homology
BLAST of Lag0025019 vs. NCBI nr
Match: XP_023876296.1 (uncharacterized protein LOC111988743 [Quercus suber])

HSP 1 Score: 88.2 bits (217), Expect = 7.2e-14
Identity = 53/177 (29.94%), Postives = 94/177 (53.11%), Query Frame = 0

Query: 1   MSVDKFEEFVVMSRWVWSRRNKELFE-------------EVVMGEKGDSGE-------GV 60
           ++ D+FE F+V +  +W++RN  +                  + E   S +        +
Sbjct: 170 LTTDEFELFLVQAWLIWNQRNTVVHRGKMRDPRWLNKRAREYLSEFQQSQDQLNILLPRL 229

Query: 61  EAVCWIPPEFPNYKLNTDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEA 120
            A  W+ P  P YKLN D A+ ++LN S IGAI+ N KGEVM  +      V D +  E 
Sbjct: 230 NANIWVAPTDPVYKLNFDAAIFKELNCSGIGAIIRNGKGEVMAAMSAKGPLVEDSEEAET 289

Query: 121 MAIREGLAAVVDAGFSRVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELKF 158
           +A R+ +   +DAGFS +V+E D+++V+      G +LS++G +V++++ ++  L++
Sbjct: 290 LACRKAVEFAIDAGFSELVIEGDNAAVMASLSHGGPDLSRLGHVVQDVQWLATGLRW 346

BLAST of Lag0025019 vs. NCBI nr
Match: XP_023885847.1 (keratin, type I cytoskeletal 13-like [Quercus suber])

HSP 1 Score: 86.3 bits (212), Expect = 2.7e-13
Identity = 54/176 (30.68%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 6   FEEFVVMSRWVWSRRNKELFEEVVMGEKGDSGEGVE---------------------AVC 65
           FE F+V S  +W++RN+ +    +M  +G +   ++                      + 
Sbjct: 82  FELFLVQSWLIWNQRNRVVHGGQMMDPRGLNKRALDYLDEYKKSHVQLAATPVMAPARIS 141

Query: 66  WIPPEFPNYKLNTDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEAMAIR 125
           W PP    YKLN D A+  DLN S  GAI+ NE+GEVM  +       L+    EA+A R
Sbjct: 142 WKPPPTSTYKLNFDAAIFADLNCSGFGAIIRNEEGEVMAGMSVKGPLSLNSAEAEALACR 201

Query: 126 EGLAAVVDAGFSRVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELKFCEV 161
           + +   ++AGFSR+V+E D++ V+    S   N S +G + E+I+ + R L++  +
Sbjct: 202 KAVQFALEAGFSRLVIEGDNALVMNAISSSAENNSLLGHIFEDIQHLIRGLQYVSI 257

BLAST of Lag0025019 vs. NCBI nr
Match: XP_030969688.1 (uncharacterized protein LOC115989965 [Quercus lobata])

HSP 1 Score: 85.5 bits (210), Expect = 4.7e-13
Identity = 55/160 (34.38%), Postives = 88/160 (55.00%), Query Frame = 0

Query: 4   DKFEEFVVMSRWVWSRRNKELFEEVVMGEKGDSGEGV-------EAVCWIPPEFPNYKLN 63
           ++ E F+V +  +W++RN  L  E+ M E   + + +       E   W PP  P +KLN
Sbjct: 73  EEVELFLVQAWIIWNQRNSVLRAEIWMEEIAQAQQHLSITPERHEGNVWRPPPPPFFKLN 132

Query: 64  TDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEAMAIREGLAAVVDAGFS 123
            D A+ ++ + S  GA++ NE+GEVM  L  +   V   +  E MA R+ +   VDAGFS
Sbjct: 133 FDAAIIKERSTSGFGAMIRNEQGEVMAALSAIGPSVSCSEEAETMACRKAVEFTVDAGFS 192

Query: 124 RVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELK 157
            +++E DS +V+K   S   +LS  G +V +I+ I R L+
Sbjct: 193 ELMIEGDSVNVMKAISSSIQDLSVAGNVVADIQWIIRGLR 232

BLAST of Lag0025019 vs. NCBI nr
Match: XP_030932920.1 (uncharacterized protein LOC115958649 [Quercus lobata])

HSP 1 Score: 85.1 bits (209), Expect = 6.1e-13
Identity = 51/116 (43.97%), Postives = 65/116 (56.03%), Query Frame = 0

Query: 45  WIPPEFPNYKLNTDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEAMAIR 104
           W PP    YKLN D A   DL  + IGAIV NEKGEVM  +      V   D  E +A R
Sbjct: 38  WKPPPSEAYKLNFDAATFSDLGRTGIGAIVRNEKGEVMAAMFACGPAVQTSDEAELLACR 97

Query: 105 EGLAAVVDAGFSRVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELKFCEV 161
             L   VDAGFSR+++E D+S+V+    S   N S  G +V++IR + R L + EV
Sbjct: 98  RALEFAVDAGFSRLIIEGDNSNVIHDISSLEENTSSFGNVVDDIRHLIRGLIWSEV 153

BLAST of Lag0025019 vs. NCBI nr
Match: XP_023928118.1 (uncharacterized protein LOC112039474 [Quercus suber])

HSP 1 Score: 84.7 bits (208), Expect = 7.9e-13
Identity = 54/176 (30.68%), Postives = 90/176 (51.14%), Query Frame = 0

Query: 1   MSVDKFEEFVVMSRWVWSRRN------------------KELFEEV--VMGEKGDSGEGV 60
           +S+++FE FV+ S  +W++RN                  KE  +E     G+       +
Sbjct: 90  LSINEFELFVIQSWIIWNQRNTVVFGGKLKDPKWLNKWAKEFLDEFHQAQGQLRIPVSCL 149

Query: 61  EAVCWIPPEFPNYKLNTDIAVNRDLNLSNIGAIVINEKGEVMLTLMRLVEFVLDVDVLEA 120
               W PP    +KLN D  V   L+ S +GA++ NE GEVM T++  V  V+D  V E 
Sbjct: 150 GDSVWRPPPDSTFKLNFDAVVFSKLSCSGVGAMIRNENGEVMATMLARVPHVVDSVVAEV 209

Query: 121 MAIREGLAAVVDAGFSRVVVETDSSSVVKQFQSDGYNLSKMGFLVEEIRGISRELK 157
           +A R  +     AGF+ +VVE D+ SV+K   +   +LS +G ++++I+ ++R  +
Sbjct: 210 IACRRAMKFACKAGFTDLVVEGDNLSVMKSLTTSETDLSWLGHIIQDIKWLTRSFR 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023876296.17.2e-1429.94uncharacterized protein LOC111988743 [Quercus suber][more]
XP_023885847.12.7e-1330.68keratin, type I cytoskeletal 13-like [Quercus suber][more]
XP_030969688.14.7e-1334.38uncharacterized protein LOC115989965 [Quercus lobata][more]
XP_030932920.16.1e-1343.97uncharacterized protein LOC115958649 [Quercus lobata][more]
XP_023928118.17.9e-1330.68uncharacterized protein LOC112039474 [Quercus suber][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 52..164
e-value: 4.4E-9
score: 38.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 56..163
e-value: 4.6E-16
score: 58.8
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 32..149
NoneNo IPR availablePANTHERPTHR47074:SF3OS07G0513450 PROTEINcoord: 32..149
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 55..163
e-value: 5.01413E-11
score: 55.0128
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 52..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0025019.1Lag0025019.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity