Lag0024551 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024551
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncytochrome P450 71A1-like
Locationchr10: 3865605 .. 3866600 (-)
RNA-Seq ExpressionLag0024551
SyntenyLag0024551
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAACTTTGGCAACAACTTATATCAATCTCCTCTCTTTGCCTTCTCTTCTTCTTCTTTCTTTCCCTTAAGCCCTTCAAAGCCAAAAACACCAACAACAACAATTCCCCTCCTTCGCCACCAAAACTTCCCATCATCGGCAACCTTCACCAACTCGGAGCCTTGCCACACCGATCACTGGCATCTCTTTCACACAAATATGGGCCTCTCATGCTCTTAAAGCTTGGCCAGTTCCCAACTTTCGTAGTTTCATCCCCAAAAATGGCCGAACAAGTGATGAAAACCCACGAAATCCAATTCTCCAACAGGCCACAAACCAAAGCCGCAAAGGCCTTCCTTTACCAATGCCAAGACGTGGGTTTTGCTCCTTATGGTGAGTACTGGCGACAGGCCAGAAAAATCTGCGCTCTCCAGCTTTTCAGTGCCAAAAGAGTGGAGTCGTTTCAGTTCGTGAGAGATGAGGAAGCTGATGTGCTTGTCAGTAGGATTCGTGAGGCTTGTGTTGGCGGCGAAGTCGTGAATCTTAACCAGTTGTTCTTGTTGGTCTCGAACAATAATATAGTGTCGAGATGCGTTTTGGGAGAGAAGTTTGAGGATGAAAATGGGAAGATGAGTAGATTTGGGGTTAGGTCGAGGAAGCTTATGGTGTTGATGACCAAGTTTTGTGTTGAGGATTTCTTCCCTTGTCTTTGGTGGATCGATGTGGTTAGAGGGTTACTTGATAGGCATCTTAAAGAGTCTTTTGAATCGATGGATGAGTTTTGTGATGAGGTGATTGAAGAACATAGGGTAAAGATGAGAGGTGGTGATCATGATCAGTGTGATGAAGCTAGAAAGGATTTTGTTGATATCATGTTGGAGCTGCAACGAGATGACATCTTGATTACAATTTCTCCCAAGAAAACCTCAAAGCAATTGTATTGGTTTGTTTTCTCTACTTCTCTTCTAATTTCTCGTATAACGATATTTGGTTGTATTGTATTTCGGTGGTAG

mRNA sequence

ATGGATCAACTTTGGCAACAACTTATATCAATCTCCTCTCTTTGCCTTCTCTTCTTCTTCTTTCTTTCCCTTAAGCCCTTCAAAGCCAAAAACACCAACAACAACAATTCCCCTCCTTCGCCACCAAAACTTCCCATCATCGGCAACCTTCACCAACTCGGAGCCTTGCCACACCGATCACTGGCATCTCTTTCACACAAATATGGGCCTCTCATGCTCTTAAAGCTTGGCCAGTTCCCAACTTTCGTAGTTTCATCCCCAAAAATGGCCGAACAAGTGATGAAAACCCACGAAATCCAATTCTCCAACAGGCCACAAACCAAAGCCGCAAAGGCCTTCCTTTACCAATGCCAAGACGTGGGTTTTGCTCCTTATGGTGAGTACTGGCGACAGGCCAGAAAAATCTGCGCTCTCCAGCTTTTCAGTGCCAAAAGAGTGGAGTCGTTTCAGTTCGTGAGAGATGAGGAAGCTGATGTGCTTGTCAGTAGGATTCGTGAGGCTTGTGTTGGCGGCGAAGTCGTGAATCTTAACCAGTTGTTCTTGTTGGTCTCGAACAATAATATAGTGTCGAGATGCGTTTTGGGAGAGAAGTTTGAGGATGAAAATGGGAAGATGAGTAGATTTGGGGTTAGGTCGAGGAAGCTTATGGTGTTGATGACCAAGTTTTGTGTTGAGGATTTCTTCCCTTGTCTTTGGTGGATCGATGTGGTTAGAGGGTTACTTGATAGGCATCTTAAAGAGTCTTTTGAATCGATGGATGAGTTTTGTGATGAGGTGATTGAAGAACATAGGGTAAAGATGAGAGGTGGTGATCATGATCAGTGTGATGAAGCTAGAAAGGATTTTGTTGATATCATGTTGGAGCTGCAACGAGATGACATCTTGATTACAATTTCTCCCAAGAAAACCTCAAAGCAATTGTATTGGTTTGTTTTCTCTACTTCTCTTCTAATTTCTCGTATAACGATATTTGGTTGTATTGTATTTCGGTGGTAG

Coding sequence (CDS)

ATGGATCAACTTTGGCAACAACTTATATCAATCTCCTCTCTTTGCCTTCTCTTCTTCTTCTTTCTTTCCCTTAAGCCCTTCAAAGCCAAAAACACCAACAACAACAATTCCCCTCCTTCGCCACCAAAACTTCCCATCATCGGCAACCTTCACCAACTCGGAGCCTTGCCACACCGATCACTGGCATCTCTTTCACACAAATATGGGCCTCTCATGCTCTTAAAGCTTGGCCAGTTCCCAACTTTCGTAGTTTCATCCCCAAAAATGGCCGAACAAGTGATGAAAACCCACGAAATCCAATTCTCCAACAGGCCACAAACCAAAGCCGCAAAGGCCTTCCTTTACCAATGCCAAGACGTGGGTTTTGCTCCTTATGGTGAGTACTGGCGACAGGCCAGAAAAATCTGCGCTCTCCAGCTTTTCAGTGCCAAAAGAGTGGAGTCGTTTCAGTTCGTGAGAGATGAGGAAGCTGATGTGCTTGTCAGTAGGATTCGTGAGGCTTGTGTTGGCGGCGAAGTCGTGAATCTTAACCAGTTGTTCTTGTTGGTCTCGAACAATAATATAGTGTCGAGATGCGTTTTGGGAGAGAAGTTTGAGGATGAAAATGGGAAGATGAGTAGATTTGGGGTTAGGTCGAGGAAGCTTATGGTGTTGATGACCAAGTTTTGTGTTGAGGATTTCTTCCCTTGTCTTTGGTGGATCGATGTGGTTAGAGGGTTACTTGATAGGCATCTTAAAGAGTCTTTTGAATCGATGGATGAGTTTTGTGATGAGGTGATTGAAGAACATAGGGTAAAGATGAGAGGTGGTGATCATGATCAGTGTGATGAAGCTAGAAAGGATTTTGTTGATATCATGTTGGAGCTGCAACGAGATGACATCTTGATTACAATTTCTCCCAAGAAAACCTCAAAGCAATTGTATTGGTTTGTTTTCTCTACTTCTCTTCTAATTTCTCGTATAACGATATTTGGTTGTATTGTATTTCGGTGGTAG

Protein sequence

MDQLWQQLISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDILITISPKKTSKQLYWFVFSTSLLISRITIFGCIVFRW
Homology
BLAST of Lag0024551 vs. NCBI nr
Match: XP_023542641.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.5 bits (968), Expect = 1.2e-100
Identity = 195/296 (65.88%), Postives = 236/296 (79.73%), Query Frame = 0

Query: 1   MDQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 60
           + Q +Q L  IS SLCL FFF + LK FK    N  N PPSPP+LPIIGNLHQLGALPH+
Sbjct: 22  IQQQFQALNPISISLCLFFFFIVLLKLFK---RNKPNLPPSPPRLPIIGNLHQLGALPHQ 81

Query: 61  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 120
           S+A+LS+KYGPLMLLKLGQ    VVSSP+MA +VMKTH+I+FSNRPQT AA   LYQCQD
Sbjct: 82  SMAALSNKYGPLMLLKLGQKHALVVSSPQMAREVMKTHDIKFSNRPQTTAAYELLYQCQD 141

Query: 121 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQL 180
           +GFA YGEYWRQARK+CA++LFSAKRV+SFQ+VRDEE   L+ RI +ACVGGE VNL+QL
Sbjct: 142 IGFAQYGEYWRQARKVCAVELFSAKRVDSFQYVRDEEVGGLMDRIGKACVGGEAVNLSQL 201

Query: 181 FLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRG 240
           FL  S NNIVSRCVLG KFE ENG  SRFG  ++KL++L+T FCV DFFPCLWWID +RG
Sbjct: 202 FLQTS-NNIVSRCVLGGKFEGENGN-SRFGDTTKKLLLLLTAFCVADFFPCLWWIDTIRG 261

Query: 241 LLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
             ++ LK+ F ++D F  EV+EEH+ K+R G     DE++KDFVDIML+LQ+DD++
Sbjct: 262 -FNKELKDCFTTLDTFFSEVVEEHKAKIRDG--VGTDESKKDFVDIMLQLQQDDMV 309

BLAST of Lag0024551 vs. NCBI nr
Match: XP_022954771.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 1.2e-100
Identity = 197/296 (66.55%), Postives = 235/296 (79.39%), Query Frame = 0

Query: 1   MDQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 60
           + Q +Q L  IS SLCL FFF L LK F+ K     N PPSPP+LPIIGNLHQLGALPH+
Sbjct: 7   IQQQFQALNPISLSLCLFFFFVLLLKLFERKKP---NFPPSPPRLPIIGNLHQLGALPHQ 66

Query: 61  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 120
           S+A+LS+KYGPLMLLKLGQ    VVSSPKMA +VMKTH+I+FSNRPQT AA   LYQCQD
Sbjct: 67  SMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELLYQCQD 126

Query: 121 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQL 180
           +GFA YGEYWRQARK+CAL+LFS KRV+SFQ+VRDEE   L+ RIR+ACVGGE VNL+QL
Sbjct: 127 IGFAQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAVNLSQL 186

Query: 181 FLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRG 240
           FL  S NNIVSRCVLGEKFEDENG  SRFGV  R+ MVL+  FCV D FP LWWID++RG
Sbjct: 187 FLQTS-NNIVSRCVLGEKFEDENGN-SRFGVTVRRAMVLIVAFCVADLFPSLWWIDIIRG 246

Query: 241 LLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
             ++ LK+  +++D F  +VIEEH+ K+R G     DE++KDFVDIML+LQ+DD++
Sbjct: 247 -FNKELKDCSKTLDTFVSKVIEEHKEKIRDG--VGTDESKKDFVDIMLQLQQDDMV 294

BLAST of Lag0024551 vs. NCBI nr
Match: XP_022137055.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 376.7 bits (966), Expect = 2.0e-100
Identity = 199/299 (66.56%), Postives = 235/299 (78.60%), Query Frame = 0

Query: 3   QLWQQL---ISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 62
           Q WQQL   +   SLCL+FFF + LK FK KN N  N PPSPPKLPIIGNLHQLG+LPH+
Sbjct: 9   QFWQQLHFDLIPFSLCLIFFFLVLLKLFKPKN-NTKNFPPSPPKLPIIGNLHQLGSLPHQ 68

Query: 63  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 122
           SLA+LS KYGPLMLLKLG   T VVSS KMA ++MKTH+++FSNRPQT  AK  LY  +D
Sbjct: 69  SLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKTLLYGFKD 128

Query: 123 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGE---VVNL 182
           VGFAPYGEYWRQARKICAL+LFSAKRVESFQ+VRDEE  VLV+RIR+ CV G+    VNL
Sbjct: 129 VGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGDGEGSVNL 188

Query: 183 NQLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDV 242
           +QLFL  S NNIVSRC+LGEKFEDENGK SRFG  SR++MVL+T FC  DFFP LWWIDV
Sbjct: 189 SQLFLSTS-NNIVSRCILGEKFEDENGK-SRFGDTSRRVMVLITAFCFADFFPALWWIDV 248

Query: 243 VRGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
           VRG  +  LK+ F ++D F  +V+EEH+ KMR G   + DE++KDFV IML+L + D++
Sbjct: 249 VRG-FNGELKKWFTTLDAFLSKVVEEHKAKMRAG--GESDESKKDFVGIMLQLMQSDMV 301

BLAST of Lag0024551 vs. NCBI nr
Match: XP_022954566.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 1.0e-99
Identity = 194/296 (65.54%), Postives = 234/296 (79.05%), Query Frame = 0

Query: 1   MDQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 60
           + Q +Q L  IS SLCL FFF L LK FK K     N PPSPP+LPIIGNLHQLGALPH+
Sbjct: 22  IQQQFQALNPISFSLCLFFFFILLLKIFKRKKP---NFPPSPPRLPIIGNLHQLGALPHQ 81

Query: 61  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 120
           S+A+LS+KYGPLMLLKLGQ    VVSSPKMA +VMKTH+I+FSNRPQT AA   LYQCQD
Sbjct: 82  SMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAYELLYQCQD 141

Query: 121 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQL 180
           +GFA YGEYWRQARK+CAL+LFSAKRV+SF +VRDEE   L+ RIR+ACVGGE VNL+QL
Sbjct: 142 IGFAQYGEYWRQARKVCALELFSAKRVDSFPYVRDEEVGGLMDRIRKACVGGEAVNLSQL 201

Query: 181 FLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRG 240
           FL  S NNIVSRCVLG KFE EN   SRFG  ++K ++L+T FCV DFFPCLWWID +RG
Sbjct: 202 FLQTS-NNIVSRCVLGGKFEGEN---SRFGDTTKKFLLLLTAFCVADFFPCLWWIDTIRG 261

Query: 241 LLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
             ++ LK+ F+++D    +V+EEH+ K+R G     DE++KDFVDIML+LQ+DD++
Sbjct: 262 -FNKELKDCFKTLDTIFSKVVEEHKAKIRDG--VLTDESKKDFVDIMLQLQQDDMV 307

BLAST of Lag0024551 vs. NCBI nr
Match: XP_022137054.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 371.7 bits (953), Expect = 6.6e-99
Identity = 195/299 (65.22%), Postives = 234/299 (78.26%), Query Frame = 0

Query: 2   DQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRS 61
           +Q+WQQ   I  SLC+ FFF + LK  K KN N  N PPSPPKLPIIGNLHQLG+LPH+S
Sbjct: 8   EQIWQQFDLIPFSLCIFFFFLVLLKLLKPKN-NTKNFPPSPPKLPIIGNLHQLGSLPHQS 67

Query: 62  LASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDV 121
           LA+LS K+GPLM LKLGQ PT VVSS +MA +VMKTH++ FS+RPQT AAK  LY  +DV
Sbjct: 68  LAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTAAKILLYGFKDV 127

Query: 122 GFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGE---VVNLN 181
           GFAPYGEYWRQARKICAL+LFSAKRVESFQ+VRDEE  VLV+RIR  CVGG+    VNL 
Sbjct: 128 GFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCVGGDGEGSVNLA 187

Query: 182 QLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVV 241
           QLFL  S NNIVSRC++G+KFEDENGK SRFG  SR++MVL+T FC  DFFP LWWIDVV
Sbjct: 188 QLFLSTS-NNIVSRCMMGDKFEDENGK-SRFGDISRRVMVLLTAFCFADFFPALWWIDVV 247

Query: 242 RGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDILI 297
           RG  +  LK+ F ++D F  +V+EEH+ KM GG  + CDE+++DF+D ML+L   D ++
Sbjct: 248 RG-FNGELKKCFTTLDAFFSKVVEEHKAKMIGGG-ELCDESKRDFMDSMLQLILQDDMV 301

BLAST of Lag0024551 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 8.1e-68
Identity = 137/316 (43.35%), Postives = 200/316 (63.29%), Query Frame = 0

Query: 8   LISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHK 67
           L+S+  L +   FFL LK    K     N PPSPP LPIIGNLHQLG LPHRSL SL+++
Sbjct: 4   LVSLLFLAIALTFFL-LK-LNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANE 63

Query: 68  YGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGE 127
            GPL+LL LG  PT +VS+ ++AE+++KTH++ F++RP T AA+   Y C DV F+PYGE
Sbjct: 64  LGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGE 123

Query: 128 YWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNN 187
           YWRQ RKIC L+L S KRV S++ +R+EE  +++ RI ++C  GE VNL++L LL+S+  
Sbjct: 124 YWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGT 183

Query: 188 IVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKE 247
           I +R   G+K+E E  + ++F   + +L  LM  F V D+FP   W+DV+ G +D  LK 
Sbjct: 184 I-TRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTG-MDARLKR 243

Query: 248 SFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDILITISPKKTSKQL 307
           +   +D F D VI++H +  +    D  ++  KD VD++L LQ+D  L     +   K +
Sbjct: 244 NHGELDAFVDHVIDDHLLSRKANGSDGVEQ--KDLVDVLLHLQKDSSLGVHLNRNNLKAV 303

Query: 308 YWFVFSTSLLISRITI 324
              +FS     + +T+
Sbjct: 304 ILDMFSGGTDTTAVTL 313

BLAST of Lag0024551 vs. ExPASy Swiss-Prot
Match: A0A068Q609 (Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP71AN24 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.5e-64
Identity = 137/309 (44.34%), Postives = 186/309 (60.19%), Query Frame = 0

Query: 2   DQLWQQ-----------LISISSLCLLFFFFLSLKPFKAKNTNNN--NSPPSPPKLPIIG 61
           +Q+WQ+           +  +  LCL  F   SL    + +  N     PPSPP+LP IG
Sbjct: 8   NQIWQEGQLQSSTSSFNIFLVPILCLSIFILFSLTRSSSPSEKNRKLKLPPSPPRLPWIG 67

Query: 62  NLHQLGALPHRSLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTK 121
           NLHQLG+ PHRSL +LS KYG +M +  G+ PT +VSS +MA+ VMKT +I F +RPQT 
Sbjct: 68  NLHQLGSFPHRSLRALSKKYGDVMFMHFGKVPTLIVSSAEMAKDVMKTQDIVFCSRPQTT 127

Query: 122 AAKAFLYQCQDVGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIRE-- 181
           A     Y   D+ FAPYGEYWRQ R+IC L+L S KRV  FQ+ R EE   LVS+IR+  
Sbjct: 128 APSILFYDGHDIAFAPYGEYWRQVRRICVLELLSLKRVHQFQYARVEEVAELVSKIRKAS 187

Query: 182 ACVGGEVVNLNQLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVED 241
           A   G  +NL +L L+ ++NNI+ RC+LG+KFED+      FG  +++LM  +  F   D
Sbjct: 188 ASANGAPINLGEL-LVSTSNNIICRCILGQKFEDKEDNW--FGETTKELMTQVMSFSFGD 247

Query: 242 FFPCLWWIDVVRGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIM 296
           FFP L WID  RG L  +LK  +   D+F D++I+EH+   + G        +KD VDI+
Sbjct: 248 FFPSLKWIDRARGYL-AYLKSIWLEFDKFFDKLIDEHKAAQKEG-----KPRKKDIVDIL 307

BLAST of Lag0024551 vs. ExPASy Swiss-Prot
Match: P37117 (Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.6e-55
Identity = 125/287 (43.55%), Postives = 172/287 (59.93%), Query Frame = 0

Query: 4   LWQQLISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLAS 63
           LW  L+    L LLF F L    F    T N   PPSP KLPIIGNLHQLG+ PHRSL  
Sbjct: 6   LWYSLL---ILLLLFIFLLIHHCFTTSKTQNMFLPPSPRKLPIIGNLHQLGSHPHRSLRK 65

Query: 64  LSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFA 123
           LS KYGP+MLL LG  P  V SS   A  ++KTH+  ++ RP+   A + LY  +DVGF+
Sbjct: 66  LSQKYGPVMLLHLGSKPVIVASSVDAARDILKTHDHVWATRPKYSIADSLLYGSKDVGFS 125

Query: 124 PYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLV 183
           P+GEYW Q R I  L L S KRV+S++ VR+EE   ++ +IR+ C    V+NL +  L  
Sbjct: 126 PFGEYWWQVRSIVVLHLLSNKRVQSYRDVREEETANMIEKIRQGC-DASVINLGE-HLCF 185

Query: 184 SNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDR 243
             NNI SR  LG  +++    +    +  + L +L T F V D+ P L W++ + G LD 
Sbjct: 186 LTNNITSRVALGRTYDERESGIDAKDILEQFLQLLDT-FNVGDYIPWLKWVNKITG-LDT 245

Query: 244 HLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQ 291
            +++  + +D F D VIEEH ++ +  ++   DEA KDFVD++LE+Q
Sbjct: 246 KVEKIAKKLDTFLDSVIEEHIIRNKKEEYAITDEA-KDFVDVLLEIQ 284

BLAST of Lag0024551 vs. ExPASy Swiss-Prot
Match: Q9STL1 (Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.0e-54
Identity = 119/287 (41.46%), Postives = 178/287 (62.02%), Query Frame = 0

Query: 6   QQLISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLS 65
           + +I I  L L+ F  + L   K K    +N+P SPP+LP+IGNLHQLG  PHRSL SLS
Sbjct: 2   ESMIRIILLSLIIFITI-LFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLS 61

Query: 66  HKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPY 125
           ++YGPLMLL+ G  P  VVSS  +A  ++KT++  F++RP++K  +   Y+ +DV  APY
Sbjct: 62  NRYGPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPY 121

Query: 126 GEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSN 185
           GEYWRQ + +C L L + K V SF+ VR EE  +++ +I+++      VNL++L L    
Sbjct: 122 GEYWRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKS--SSLQVNLSEL-LGSLT 181

Query: 186 NNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHL 245
           N+++SR  LG K+ DE    + F    ++L  L+ +FCV  + P L WID + G LD  L
Sbjct: 182 NDVISRVALGRKYSDE----TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISG-LDGQL 241

Query: 246 KESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRD 293
           K++   +DEF ++V++         DH+  D  R DFVD++L +QR+
Sbjct: 242 KKTGNDLDEFLEKVVQ---------DHEDGDAQRTDFVDVLLRIQRE 270

BLAST of Lag0024551 vs. ExPASy Swiss-Prot
Match: P58046 (Cytochrome P450 71A15 OS=Arabidopsis thaliana OX=3702 GN=CYP71A15 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.9e-54
Identity = 118/284 (41.55%), Postives = 174/284 (61.27%), Query Frame = 0

Query: 9   ISISSLCL-LFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHK 68
           I I SLCL     FL LKP   +    +N PPSP ++P+IGNLHQL   PHRSL SLSH+
Sbjct: 3   IIIISLCLATILAFLLLKPLLNRTVAKDNLPPSPWRVPVIGNLHQLSLHPHRSLRSLSHR 62

Query: 69  YGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGE 128
           YGPLMLL  G+ P  VVSS  +A  +MKTH+++ +NRP+ K  +  L   ++V F+PYG+
Sbjct: 63  YGPLMLLHFGRVPILVVSSSDVAHDLMKTHDLKVANRPRLKVIETILNGGREVVFSPYGD 122

Query: 129 YWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNN 188
           YWRQ + +C + L + K V+SF  VR+EE  V++ ++ +A      +NL++L L+   ++
Sbjct: 123 YWRQIKTVCVVHLLNKKMVQSFAKVREEERSVMMEKVEKASSDSSPLNLSKL-LITLTSD 182

Query: 189 IVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKE 248
           + SR   G+K  +E   MS F  + RK+  L+  F V ++ PCL WID +RGL +R  +E
Sbjct: 183 VASRVSFGKKHSNE-ASMSDFKNQVRKITELVGGFPVSEYIPCLAWIDQIRGLYNR-AEE 242

Query: 249 SFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQR 292
             +   +  D+V++EH         D  ++  KDFVDI+L  +R
Sbjct: 243 VSKIFGDLMDKVVQEHL--------DATNKPTKDFVDILLSFER 275

BLAST of Lag0024551 vs. ExPASy TrEMBL
Match: A0A6J1GRT5 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 5.8e-101
Identity = 197/296 (66.55%), Postives = 235/296 (79.39%), Query Frame = 0

Query: 1   MDQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 60
           + Q +Q L  IS SLCL FFF L LK F+ K     N PPSPP+LPIIGNLHQLGALPH+
Sbjct: 7   IQQQFQALNPISLSLCLFFFFVLLLKLFERKKP---NFPPSPPRLPIIGNLHQLGALPHQ 66

Query: 61  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 120
           S+A+LS+KYGPLMLLKLGQ    VVSSPKMA +VMKTH+I+FSNRPQT AA   LYQCQD
Sbjct: 67  SMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAHELLYQCQD 126

Query: 121 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQL 180
           +GFA YGEYWRQARK+CAL+LFS KRV+SFQ+VRDEE   L+ RIR+ACVGGE VNL+QL
Sbjct: 127 IGFAQYGEYWRQARKVCALELFSVKRVDSFQYVRDEEVGGLMDRIRKACVGGEAVNLSQL 186

Query: 181 FLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRG 240
           FL  S NNIVSRCVLGEKFEDENG  SRFGV  R+ MVL+  FCV D FP LWWID++RG
Sbjct: 187 FLQTS-NNIVSRCVLGEKFEDENGN-SRFGVTVRRAMVLIVAFCVADLFPSLWWIDIIRG 246

Query: 241 LLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
             ++ LK+  +++D F  +VIEEH+ K+R G     DE++KDFVDIML+LQ+DD++
Sbjct: 247 -FNKELKDCSKTLDTFVSKVIEEHKEKIRDG--VGTDESKKDFVDIMLQLQQDDMV 294

BLAST of Lag0024551 vs. ExPASy TrEMBL
Match: A0A6J1C5L5 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 9.9e-101
Identity = 199/299 (66.56%), Postives = 235/299 (78.60%), Query Frame = 0

Query: 3   QLWQQL---ISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 62
           Q WQQL   +   SLCL+FFF + LK FK KN N  N PPSPPKLPIIGNLHQLG+LPH+
Sbjct: 9   QFWQQLHFDLIPFSLCLIFFFLVLLKLFKPKN-NTKNFPPSPPKLPIIGNLHQLGSLPHQ 68

Query: 63  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 122
           SLA+LS KYGPLMLLKLG   T VVSS KMA ++MKTH+++FSNRPQT  AK  LY  +D
Sbjct: 69  SLAALSDKYGPLMLLKLGHAQTLVVSSAKMARELMKTHDLKFSNRPQTMPAKTLLYGFKD 128

Query: 123 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGE---VVNL 182
           VGFAPYGEYWRQARKICAL+LFSAKRVESFQ+VRDEE  VLV+RIR+ CV G+    VNL
Sbjct: 129 VGFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVSVLVNRIRKGCVDGDGEGSVNL 188

Query: 183 NQLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDV 242
           +QLFL  S NNIVSRC+LGEKFEDENGK SRFG  SR++MVL+T FC  DFFP LWWIDV
Sbjct: 189 SQLFLSTS-NNIVSRCILGEKFEDENGK-SRFGDTSRRVMVLITAFCFADFFPALWWIDV 248

Query: 243 VRGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
           VRG  +  LK+ F ++D F  +V+EEH+ KMR G   + DE++KDFV IML+L + D++
Sbjct: 249 VRG-FNGELKKWFTTLDAFLSKVVEEHKAKMRAG--GESDESKKDFVGIMLQLMQSDMV 301

BLAST of Lag0024551 vs. ExPASy TrEMBL
Match: A0A6J1GR89 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456801 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.9e-100
Identity = 194/296 (65.54%), Postives = 234/296 (79.05%), Query Frame = 0

Query: 1   MDQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHR 60
           + Q +Q L  IS SLCL FFF L LK FK K     N PPSPP+LPIIGNLHQLGALPH+
Sbjct: 22  IQQQFQALNPISFSLCLFFFFILLLKIFKRKKP---NFPPSPPRLPIIGNLHQLGALPHQ 81

Query: 61  SLASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQD 120
           S+A+LS+KYGPLMLLKLGQ    VVSSPKMA +VMKTH+I+FSNRPQT AA   LYQCQD
Sbjct: 82  SMAALSNKYGPLMLLKLGQKHALVVSSPKMAREVMKTHDIKFSNRPQTTAAYELLYQCQD 141

Query: 121 VGFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQL 180
           +GFA YGEYWRQARK+CAL+LFSAKRV+SF +VRDEE   L+ RIR+ACVGGE VNL+QL
Sbjct: 142 IGFAQYGEYWRQARKVCALELFSAKRVDSFPYVRDEEVGGLMDRIRKACVGGEAVNLSQL 201

Query: 181 FLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRG 240
           FL  S NNIVSRCVLG KFE EN   SRFG  ++K ++L+T FCV DFFPCLWWID +RG
Sbjct: 202 FLQTS-NNIVSRCVLGGKFEGEN---SRFGDTTKKFLLLLTAFCVADFFPCLWWIDTIRG 261

Query: 241 LLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
             ++ LK+ F+++D    +V+EEH+ K+R G     DE++KDFVDIML+LQ+DD++
Sbjct: 262 -FNKELKDCFKTLDTIFSKVVEEHKAKIRDG--VLTDESKKDFVDIMLQLQQDDMV 307

BLAST of Lag0024551 vs. ExPASy TrEMBL
Match: A0A6J1C631 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 3.2e-99
Identity = 195/299 (65.22%), Postives = 234/299 (78.26%), Query Frame = 0

Query: 2   DQLWQQLISIS-SLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRS 61
           +Q+WQQ   I  SLC+ FFF + LK  K KN N  N PPSPPKLPIIGNLHQLG+LPH+S
Sbjct: 8   EQIWQQFDLIPFSLCIFFFFLVLLKLLKPKN-NTKNFPPSPPKLPIIGNLHQLGSLPHQS 67

Query: 62  LASLSHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDV 121
           LA+LS K+GPLM LKLGQ PT VVSS +MA +VMKTH++ FS+RPQT AAK  LY  +DV
Sbjct: 68  LAALSDKHGPLMFLKLGQAPTLVVSSAEMAREVMKTHDLNFSDRPQTTAAKILLYGFKDV 127

Query: 122 GFAPYGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGE---VVNLN 181
           GFAPYGEYWRQARKICAL+LFSAKRVESFQ+VRDEE  VLV+RIR  CVGG+    VNL 
Sbjct: 128 GFAPYGEYWRQARKICALELFSAKRVESFQYVRDEEVAVLVNRIRRGCVGGDGEGSVNLA 187

Query: 182 QLFLLVSNNNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVV 241
           QLFL  S NNIVSRC++G+KFEDENGK SRFG  SR++MVL+T FC  DFFP LWWIDVV
Sbjct: 188 QLFLSTS-NNIVSRCMMGDKFEDENGK-SRFGDISRRVMVLLTAFCFADFFPALWWIDVV 247

Query: 242 RGLLDRHLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDILI 297
           RG  +  LK+ F ++D F  +V+EEH+ KM GG  + CDE+++DF+D ML+L   D ++
Sbjct: 248 RG-FNGELKKCFTTLDAFFSKVVEEHKAKMIGGG-ELCDESKRDFMDSMLQLILQDDMV 301

BLAST of Lag0024551 vs. ExPASy TrEMBL
Match: A0A6J1GRU2 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 7.8e-98
Identity = 189/284 (66.55%), Postives = 226/284 (79.58%), Query Frame = 0

Query: 12  SSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHKYGPL 71
           SSLCL FFF + LK +K K     N PPSPP+LPIIGNLHQLGALPH+S+A+LS+KYGPL
Sbjct: 19  SSLCLFFFFIVLLKLYKRKKP---NFPPSPPRLPIIGNLHQLGALPHQSMAALSNKYGPL 78

Query: 72  MLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGEYWRQ 131
           MLLKLGQ  T VVSS KMA +VMKT +I+FSNRPQT AA   LYQCQD+GF  YGEYWRQ
Sbjct: 79  MLLKLGQKHTLVVSSAKMAREVMKTQDIKFSNRPQTTAAYELLYQCQDIGFTQYGEYWRQ 138

Query: 132 ARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNNIVSR 191
           ARK+CAL+LFSAKRV+SFQ+VRD+E   L+ RIR+ACVG E VNL+QLFL  S NNIVSR
Sbjct: 139 ARKVCALELFSAKRVDSFQYVRDDEIGGLMDRIRKACVGVEAVNLSQLFLQTS-NNIVSR 198

Query: 192 CVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKESFES 251
           CVLGEKFEDENG  SRFG  +RK+MVLM  FCV D FP LWWID +RG  ++ L++ F+ 
Sbjct: 199 CVLGEKFEDENGN-SRFGDAARKVMVLMVAFCVADLFPSLWWIDTIRG-FNKQLEDCFKR 258

Query: 252 MDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDDIL 296
           +D F  +V+EEH+ K+R G     DE++KDFVDIML+LQRD ++
Sbjct: 259 LDTFFSKVVEEHKEKIRDG--VLTDESKKDFVDIMLQLQRDGMV 294

BLAST of Lag0024551 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 215.3 bits (547), Expect = 7.3e-56
Identity = 119/287 (41.46%), Postives = 178/287 (62.02%), Query Frame = 0

Query: 6   QQLISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLS 65
           + +I I  L L+ F  + L   K K    +N+P SPP+LP+IGNLHQLG  PHRSL SLS
Sbjct: 2   ESMIRIILLSLIIFITI-LFFIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLS 61

Query: 66  HKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPY 125
           ++YGPLMLL+ G  P  VVSS  +A  ++KT++  F++RP++K  +   Y+ +DV  APY
Sbjct: 62  NRYGPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPY 121

Query: 126 GEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSN 185
           GEYWRQ + +C L L + K V SF+ VR EE  +++ +I+++      VNL++L L    
Sbjct: 122 GEYWRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKS--SSLQVNLSEL-LGSLT 181

Query: 186 NNIVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHL 245
           N+++SR  LG K+ DE    + F    ++L  L+ +FCV  + P L WID + G LD  L
Sbjct: 182 NDVISRVALGRKYSDE----TDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISG-LDGQL 241

Query: 246 KESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRD 293
           K++   +DEF ++V++         DH+  D  R DFVD++L +QR+
Sbjct: 242 KKTGNDLDEFLEKVVQ---------DHEDGDAQRTDFVDVLLRIQRE 270

BLAST of Lag0024551 vs. TAIR 10
Match: AT5G24950.1 (cytochrome P450, family 71, subfamily A, polypeptide 15 )

HSP 1 Score: 213.4 bits (542), Expect = 2.8e-55
Identity = 118/284 (41.55%), Postives = 174/284 (61.27%), Query Frame = 0

Query: 9   ISISSLCL-LFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHK 68
           I I SLCL     FL LKP   +    +N PPSP ++P+IGNLHQL   PHRSL SLSH+
Sbjct: 3   IIIISLCLATILAFLLLKPLLNRTVAKDNLPPSPWRVPVIGNLHQLSLHPHRSLRSLSHR 62

Query: 69  YGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGE 128
           YGPLMLL  G+ P  VVSS  +A  +MKTH+++ +NRP+ K  +  L   ++V F+PYG+
Sbjct: 63  YGPLMLLHFGRVPILVVSSSDVAHDLMKTHDLKVANRPRLKVIETILNGGREVVFSPYGD 122

Query: 129 YWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNN 188
           YWRQ + +C + L + K V+SF  VR+EE  V++ ++ +A      +NL++L L+   ++
Sbjct: 123 YWRQIKTVCVVHLLNKKMVQSFAKVREEERSVMMEKVEKASSDSSPLNLSKL-LITLTSD 182

Query: 189 IVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKE 248
           + SR   G+K  +E   MS F  + RK+  L+  F V ++ PCL WID +RGL +R  +E
Sbjct: 183 VASRVSFGKKHSNE-ASMSDFKNQVRKITELVGGFPVSEYIPCLAWIDQIRGLYNR-AEE 242

Query: 249 SFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQR 292
             +   +  D+V++EH         D  ++  KDFVDI+L  +R
Sbjct: 243 VSKIFGDLMDKVVQEHL--------DATNKPTKDFVDILLSFER 275

BLAST of Lag0024551 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 213.0 bits (541), Expect = 3.6e-55
Identity = 121/285 (42.46%), Postives = 176/285 (61.75%), Query Frame = 0

Query: 8   LISISSLCLLFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHK 67
           +I + SL ++F   L  K  K K    +N+P SPP+LP+IGNLHQLG  PHRSL SLSH+
Sbjct: 6   MIILQSL-IIFITILFFK--KQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHR 65

Query: 68  YGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGE 127
           YGPLMLL LG+ P  VVSS  +A  ++KTH+  F++RP++K  +   Y  +DV FAPYGE
Sbjct: 66  YGPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGE 125

Query: 128 YWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNN 187
           YWRQ + +C L+L S K V SF+ VR EE  +++ +I+++      VN+++L L    N+
Sbjct: 126 YWRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKS--SSLQVNVSEL-LGSLTND 185

Query: 188 IVSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKE 247
           ++SR  LG K+  E           ++LM+LM +F V  + P L WID + G LD  L +
Sbjct: 186 VISRIALGRKYSGETDSKELM----KRLMMLMGEFSVGTYVPWLGWIDWISG-LDGQLNK 245

Query: 248 SFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRD 293
           +   +DEF ++V++         DH   D  R DFVD++L +QR+
Sbjct: 246 TGNDLDEFLEKVVQ---------DHVDGDGQRTDFVDVLLRIQRE 270

BLAST of Lag0024551 vs. TAIR 10
Match: AT2G30750.1 (cytochrome P450, family 71, subfamily A, polypeptide 12 )

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-54
Identity = 124/305 (40.66%), Postives = 185/305 (60.66%), Query Frame = 0

Query: 7   QLISISSLCL--LFFFFLSLKPFKAKNTNNNNSPPSPPKLPIIGNLHQLGALPHRSLASL 66
           ++I + SLCL  L   FL LK F  +  N  N PPSP +LP+IGNLHQL   PHRSL SL
Sbjct: 8   EMILMVSLCLTTLITLFL-LKQFLKRTANKVNLPPSPWRLPLIGNLHQLSLHPHRSLHSL 67

Query: 67  SHKYGPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAP 126
           S +YGPLMLL  G+ P  VVSS + A++V+KTH+++F+NRP++KA    +   +DV F P
Sbjct: 68  SLRYGPLMLLHFGRVPILVVSSGEAAQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGP 127

Query: 127 YGEYWRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVS 186
           YGEYWRQ + +C L L + K V SF+ +R+EE + ++ ++ +A       NL++LF+ + 
Sbjct: 128 YGEYWRQMKSVCILNLLTNKMVASFEKIREEELNEMIKKLEKASSSSSSENLSELFVTLP 187

Query: 187 NNNIVSRCVLGEKF-EDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDR 246
            +++ SR  LG K  EDE  +  +   R R++M L+ +F + D+ P L WID + G  + 
Sbjct: 188 -SDVTSRIALGRKHSEDETARDLK--KRVRQIMELLGEFPIGDYVPALAWIDRING-FNA 247

Query: 247 HLKESFESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLEL----------QRDD 299
            +KE  +   +  D+V++EH   +  G+H      ++DFVDI+L +          QRDD
Sbjct: 248 RIKEVSQGFSDLMDKVVQEH---LEAGNH------KEDFVDILLSIESEKSIGFQAQRDD 298

BLAST of Lag0024551 vs. TAIR 10
Match: AT5G24960.1 (cytochrome P450, family 71, subfamily A, polypeptide 14 )

HSP 1 Score: 209.5 bits (532), Expect = 4.0e-54
Identity = 117/285 (41.05%), Postives = 174/285 (61.05%), Query Frame = 0

Query: 11  ISSLCL-LFFFFLSLKPFKAKN-TNNNNSPPSPPKLPIIGNLHQLGALPHRSLASLSHKY 70
           I SLCL      L LK F  +  T   N PPSP ++P+IGNLHQL   PHRSL SLSH+Y
Sbjct: 5   IISLCLATILALLLLKQFLNRTYTAKVNLPPSPWRVPVIGNLHQLSLHPHRSLRSLSHRY 64

Query: 71  GPLMLLKLGQFPTFVVSSPKMAEQVMKTHEIQFSNRPQTKAAKAFLYQCQDVGFAPYGEY 130
           GPLMLL  G+ P  VVSS  +A  +MKTH+++ +NRPQ K  +      +++ F+PYGEY
Sbjct: 65  GPLMLLHFGRVPVLVVSSSDVAHDLMKTHDLKVANRPQLKVVEKIFNGGREMVFSPYGEY 124

Query: 131 WRQARKICALQLFSAKRVESFQFVRDEEADVLVSRIREACVGGEVVNLNQLFLLVSNNNI 190
           WRQ + +C + L + K+V+SF+ VR+EE   ++ R+ +A      +NL++L LL   +++
Sbjct: 125 WRQIKSVCIVNLLNKKKVQSFEKVREEEISEMMERVEKASSDSSPLNLSEL-LLTLTSDV 184

Query: 191 VSRCVLGEKFEDENGKMSRFGVRSRKLMVLMTKFCVEDFFPCLWWIDVVRGLLDRHLKES 250
            SR  LG K+  E   MS F ++ RK+  L+  F V ++ PCL WID +RG +D   +E 
Sbjct: 185 TSRVSLGRKYSKEE-SMSDFKIQMRKITELVGGFPVGEYIPCLAWIDKLRG-VDEKAEEV 244

Query: 251 FESMDEFCDEVIEEHRVKMRGGDHDQCDEARKDFVDIMLELQRDD 294
            ++  +  ++V++EH         D  D+   DFVD++L L+R +
Sbjct: 245 SKAFGDLMEKVLQEHL--------DATDKPTLDFVDVLLSLERHE 278

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023542641.11.2e-10065.88cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo][more]
XP_022954771.11.2e-10066.55cytochrome P450 71A1-like [Cucurbita moschata][more]
XP_022137055.12.0e-10066.56cytochrome P450 71A1-like [Momordica charantia][more]
XP_022954566.11.0e-9965.54cytochrome P450 71A1-like [Cucurbita moschata][more]
XP_022137054.16.6e-9965.22cytochrome P450 71A1-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P244658.1e-6843.35Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q6092.5e-6444.34Phenylacetaldehyde oxime monooxygenase CYP71AN24 OS=Prunus mume OX=102107 GN=CYP... [more]
P371174.6e-5543.55Cytochrome P450 71A4 OS=Solanum melongena OX=4111 GN=CYP71A4 PE=2 SV=1[more]
Q9STL11.0e-5441.46Cytochrome P450 71A22 OS=Arabidopsis thaliana OX=3702 GN=CYP71A22 PE=2 SV=1[more]
P580463.9e-5441.55Cytochrome P450 71A15 OS=Arabidopsis thaliana OX=3702 GN=CYP71A15 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GRT55.8e-10166.55cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456925 PE=3 SV=... [more]
A0A6J1C5L59.9e-10166.56cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008617 PE=3 SV... [more]
A0A6J1GR894.9e-10065.54cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456801 PE=3 SV=... [more]
A0A6J1C6313.2e-9965.22cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111008616 PE=3 SV... [more]
A0A6J1GRU27.8e-9866.55cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111456926 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G48310.17.3e-5641.46cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
AT5G24950.12.8e-5541.55cytochrome P450, family 71, subfamily A, polypeptide 15 [more]
AT3G48320.13.6e-5542.46cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT2G30750.11.1e-5440.66cytochrome P450, family 71, subfamily A, polypeptide 12 [more]
AT5G24960.14.0e-5441.05cytochrome P450, family 71, subfamily A, polypeptide 14 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 65..84
score: 34.3
coord: 89..110
score: 19.95
coord: 184..202
score: 20.88
IPR001128Cytochrome P450PFAMPF00067p450coord: 38..290
e-value: 1.6E-30
score: 106.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..314
e-value: 3.2E-51
score: 176.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 38..293
NoneNo IPR availablePANTHERPTHR47955CYTOCHROME P450 FAMILY 71 PROTEINcoord: 17..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024551.1Lag0024551.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen