Lag0024377 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0024377
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	kunitz trypsin inhibitor 2
Location	chr10: 2607023 .. 2607637 (-)
RNA-Seq Expression	Lag0024377
Synteny	Lag0024377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGAAGTTTGTATTGCTCTCTTTTCTTTTCATCGCCATTGCCTCTTCTGAGGTACGCTTCTGCAGAGCCGACGCCTCGCCGGACACCGTCCTCGACACCGACGGTAAGAAGCTCCGGGCCGGCGACTACTACTACATTCTCCCGGCTTTCCACGGCAGCGGCGGCGGATTAACTATCAGCAGTATCCACGATCAATTAAGTAACGTCGTCGTCCAAGAACCTAATGAATTATCCGACGGTGTTCCGGCGACATTTGCGCCTATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCGACCGATTTGAACATCCAATTCAAGCTGACATTGAAGTGCAGCGGATCGACGGTGTGGAAATTAGGCAAATTCGATCGGTATTTGAGGCAGTATTTCGTGACGATCGGTGGAACGAAAGGAAATCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTACGGCAACGATTACAAGTTCGTGTACTGTCCAAGTGTGTGTAAGTACTGCAAGGTTATGTGCAAAGATATCGGCATTTTTAACTTCAATGGGAAGAGGGCTCTTGGTTTGAGTGATGCGCCATTCCCGGTTATGTTCAAGAAAGTTTGA

mRNA sequence

ATGAAGAAGAAGTTTGTATTGCTCTCTTTTCTTTTCATCGCCATTGCCTCTTCTGAGGTACGCTTCTGCAGAGCCGACGCCTCGCCGGACACCGTCCTCGACACCGACGGTAAGAAGCTCCGGGCCGGCGACTACTACTACATTCTCCCGGCTTTCCACGGCAGCGGCGGCGGATTAACTATCAGCAGTATCCACGATCAATTAAGTAACGTCGTCGTCCAAGAACCTAATGAATTATCCGACGGTGTTCCGGCGACATTTGCGCCTATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCGACCGATTTGAACATCCAATTCAAGCTGACATTGAAGTGCAGCGGATCGACGGTGTGGAAATTAGGCAAATTCGATCGGTATTTGAGGCAGTATTTCGTGACGATCGGTGGAACGAAAGGAAATCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTACGGCAACGATTACAAGTTCGTGTACTGTCCAAGTGTGTGTAAGTACTGCAAGGTTATGTGCAAAGATATCGGCATTTTTAACTTCAATGGGAAGAGGGCTCTTGGTTTGAGTGATGCGCCATTCCCGGTTATGTTCAAGAAAGTTTGA

Coding sequence (CDS)

ATGAAGAAGAAGTTTGTATTGCTCTCTTTTCTTTTCATCGCCATTGCCTCTTCTGAGGTACGCTTCTGCAGAGCCGACGCCTCGCCGGACACCGTCCTCGACACCGACGGTAAGAAGCTCCGGGCCGGCGACTACTACTACATTCTCCCGGCTTTCCACGGCAGCGGCGGCGGATTAACTATCAGCAGTATCCACGATCAATTAAGTAACGTCGTCGTCCAAGAACCTAATGAATTATCCGACGGTGTTCCGGCGACATTTGCGCCTATAAACCCTAAGAAAGGCGTCGTTCGAGTTTCGACCGATTTGAACATCCAATTCAAGCTGACATTGAAGTGCAGCGGATCGACGGTGTGGAAATTAGGCAAATTCGATCGGTATTTGAGGCAGTATTTCGTGACGATCGGTGGAACGAAAGGAAATCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGTACGGCAACGATTACAAGTTCGTGTACTGTCCAAGTGTGTGTAAGTACTGCAAGGTTATGTGCAAAGATATCGGCATTTTTAACTTCAATGGGAAGAGGGCTCTTGGTTTGAGTGATGCGCCATTCCCGGTTATGTTCAAGAAAGTTTGA

Protein sequence

MKKKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTISSIHDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIGIFNFNGKRALGLSDAPFPVMFKKV

Homology

[Close]

BLAST of Lag0024377 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 307.4 bits (786), Expect = 9.4e-80
Identity = 157/209 (75.12%), Postives = 168/209 (80.38%), Query Frame = 0


Query: 3   KKFVLLSFLFIA--IASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGS-GGGL 62
           KKF LLSFLFIA  IASSE+RFCRADASPD VLDTDGKKLR  + YYILPAF GS GGGL
Sbjct: 2   KKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGL 61

Query: 63  TISSI---HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGS 122
            I +I   +D+    VVQE  E SDG P TF PINPKKGVVRVSTDLNI+F  T +C  S
Sbjct: 62  AIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKS 121

Query: 123 TVWKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYG-NDYKFVYCPSVCKYCKVM 182
           TVWKLG FDRYLRQYFVTIGGTKGNPGRET ++WFKIEKYG  +YK VYCP VCKYCKVM
Sbjct: 122 TVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVM 181

Query: 183 CKDIGIFNFNGKRALGLSDAPFPVMFKKV 205
           CKDIGIF  NG R L LSD PFPV+FKKV
Sbjct: 182 CKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of Lag0024377 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 304.3 bits (778), Expect = 7.9e-79
Identity = 139/204 (68.14%), Postives = 169/204 (82.84%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           K F LL FLFI IASSEVRFCRADASPD VLDTDGKKLRAGD YYIL  +  + GGL+I 
Sbjct: 2   KNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  SI--HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWK 122
            I  +++    ++ E  +  DG+PATF+P+NPKKGVVRVSTDLNI+F+ + +C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVWK 121

Query: 123 LGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIG 182
           +GKFD+YL+QYFVT+GGTKGNPGRET+ +WFK+EK+G +YKFVYCP+VCKYCKVMCKD+G
Sbjct: 122 VGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDVG 181

Query: 183 IFNFNGKRALGLSDAPFPVMFKKV 205
           +F  NG+R   L+DAPFPVMFKKV
Sbjct: 182 LFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of Lag0024377 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 297.7 bits (761), Expect = 7.4e-77
Identity = 140/203 (68.97%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           K F LL FLF  IAS+EVR C++DASPD VLD DGKKLRAG  YYILP   G GGGLT+ 
Sbjct: 2   KNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTLG 61

Query: 63  SI-HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWKL 122
           +I HD+    VVQE  E+S+G+PATFAP+NPKKGVVRVSTDLN++F+ +  C+ STVWKL
Sbjct: 62  NIGHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTVWKL 121

Query: 123 GKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIGI 182
            KFD   RQ+ V IGG +GNPG+ETLD+WFKIEK+G DYKFV+CPSVCK+CKV+CKD+GI
Sbjct: 122 DKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKDVGI 181

Query: 183 FNFNGKRALGLSDAPFPVMFKKV 205
           F  NG RAL LSD PFPVMFKKV
Sbjct: 182 FIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of Lag0024377 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 297.4 bits (760), Expect = 9.7e-77
Identity = 140/205 (68.29%), Postives = 164/205 (80.00%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           K F LLSFLFI I SSEVRFCRAD SPD VLDTDGKKLR+ D YYIL  +  + GGL+I 
Sbjct: 2   KNFALLSFLFIVIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNSGGLSIG 61

Query: 63  SIH---DQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVW 122
           SI    ++    ++ E  E   G+ ATF PINPKKGVVRVSTDLNIQF+ + +C  STVW
Sbjct: 62  SIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGESTVW 121

Query: 123 KLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDI 182
           K+GKFD+YL+QYFVTIGG KGNPGRET+ +WFKIEKYGN+YK V+CP+VCKYCKV+CKD+
Sbjct: 122 KIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNNYKLVHCPTVCKYCKVICKDV 181

Query: 183 GIFNFNGKRALGLSDAPFPVMFKKV 205
           GIF  NGKR L L+D+PFPVMFKKV
Sbjct: 182 GIFYKNGKRVLALNDSPFPVMFKKV 206

[Close]

BLAST of Lag0024377 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 282.0 bits (720), Expect = 4.2e-72
Identity = 134/205 (65.37%), Postives = 159/205 (77.56%), Query Frame = 0


Query: 1   MKKKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLT 60
           M K F +  F+FI +AS+E+RF  ADASP+ VLD DGKKLRAG  YYILP F G GGGLT
Sbjct: 1   MTKNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLT 60

Query: 61  ISSIHDQLSNV-VVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVW 120
           + ++  ++  V VVQE  EL +G P TF P+NPKKGVVRVSTDLN+QF  +  C  STVW
Sbjct: 61  LGNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVW 120

Query: 121 KLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDI 180
           KL KFD    Q+FVTIGG++GNPG ET+D+WFKIEK+G DYK V+CP+VC +CKVMC+DI
Sbjct: 121 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDI 180

Query: 181 GIFNFNGKRALGLSDAPFPVMFKKV 205
           GIF  NGKRAL LSD PFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-47
Identity = 102/208 (49.04%), Postives = 129/208 (62.02%), Query Frame = 0


Query: 8   LSFLFIA---IASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTISSI 67
           LSF F++    A++      AD++P+ VLD DG+KLR G  YYI+P     GGGLT+S+ 
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 68  HDQLSNV----VVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKC--SGSTV 127
               + V    VVQ   E+    P  F P NPK+ VVRVSTDLNI F   + C  + STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 128 WKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKY--GNDYKFVYCPSVCKYCKVMC 187
           W+L K+D    QYFVTIGG KGNPG ET+ SWFKIE++     YK V+CP+VC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 188 KDIGIF-NFNGKRALGLSDAPFPVMFKK 204
            D+GI+ +  G+R L LSD PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.6e-44
Identity = 86/199 (43.22%), Postives = 130/199 (65.33%), Query Frame = 0


Query: 6   VLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTISSIH 65
           +L  FL +A+  S  R    +A+ + V D +GK L  G  YYILP   G GGGLT+S++ 
Sbjct: 4   LLYIFLLLAVFISH-RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLK 63

Query: 66  -DQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWKLGKF 125
            +     V+Q+  E+S G+P  F+P + K   + VSTD+NI+F      S +++W+L  F
Sbjct: 64  TETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANF 123

Query: 126 DRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIGIFNF 185
           D   +Q+F++  G +GNPG++T+D+WFKI+K+  DYK  +CP+VC +CKV+C+D+G+F  
Sbjct: 124 DETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQ 183

Query: 186 NGKRALGLSDAPFPVMFKK 204
           +GKR L LSD P  VMFK+
Sbjct: 184 DGKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 88/199 (44.22%), Postives = 115/199 (57.79%), Query Frame = 0


Query: 11  LFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGS-GGGLTISSIHDQ-L 70
           L  A  S    F  A+A+   VLDTDG +L+ G  YY+L +  G+ GGGL +     Q  
Sbjct: 10  LLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSC 69

Query: 71  SNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFK--LTLKCSGSTVWKLGKFDR 130
             +VVQ  ++L +G P  F+  + K  VVRVSTD+NI+F       CS STVW+L  +D 
Sbjct: 70  PEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDN 129

Query: 131 YLRQYFVTIGGTKGNPGRETLDSWFKIEKYG-NDYKFVYCPSVCKYCKVMCKDIGIF-NF 190
              +++VT  G KG PG  TL SWFKIEK G   YKF +CPSVC  C  +C DIG   + 
Sbjct: 130 SAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDD 189

Query: 191 NGKRALGLSDAPFPVMFKK 204
           +G+  L LSD  +  MFKK
Sbjct: 190 DGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.7e-29
Identity = 82/207 (39.61%), Postives = 112/207 (54.11%), Query Frame = 0


Query: 1   MKKKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLT 60
           M  KF L+  L   +AS+         +   V+D DG  +   + YY+LP   G GGGLT
Sbjct: 8   MNPKFYLVLALTAVLASN---------AYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLT 67

Query: 61  ISSIHDQ-LSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKL-TLKCSGSTV 120
           ++    Q     +VQE +E+ +G+P  F+    K   V  S +LNI+  +    C  ST 
Sbjct: 68  LAGRGGQPCPYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTY 127

Query: 121 WKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGND-YKFVYCPSVCKYCKVMCK 180
           W++G+FD   +QYFV  G      G+++L S+FKIEK G D YKFV+CP  C      C 
Sbjct: 128 WRVGEFDHERKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCS 187

Query: 181 DIGIF-NFNGKRALGLSDAPFPVMFKK 204
           D+GIF +  G R L LSD PF VMFKK
Sbjct: 188 DVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.4e-22
Identity = 70/207 (33.82%), Postives = 99/207 (47.83%), Query Frame = 0


Query: 1   MKKKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLT 60
           ++   +LLS L I+ + S        A+P  V DT+G +L A   YY+LPA  G GGGLT
Sbjct: 4   LRLPLILLSLLAISFSCS--------AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLT 63

Query: 61  ISSIHDQLSNVVVQEPNELSDGVPATFAP----INPKKGVVRVSTDLNIQFKLTLKCSGS 120
           ++        +V QE +E   G P  F P      P+   +RVSTD+ I+F     C  S
Sbjct: 64  MAPRVLPCPLLVAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQS 123

Query: 121 TVWKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMC 180
           T W +G  D  L      + G    P     ++ F++EKYG  YK V        C+  C
Sbjct: 124 TEWHVG--DEPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLV-------SCRDSC 183

Query: 181 KDIGIFNFNGKRALGLSDAPFPVMFKK 204
           +D+G+     +  LG S  P  V+FKK
Sbjct: 184 QDLGVSRDGARAWLGASQPPHVVVFKK 193

[Close]

BLAST of Lag0024377 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.5e-80
Identity = 157/209 (75.12%), Postives = 168/209 (80.38%), Query Frame = 0


Query: 3   KKFVLLSFLFIA--IASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGS-GGGL 62
           KKF LLSFLFIA  IASSE+RFCRADASPD VLDTDGKKLR  + YYILPAF GS GGGL
Sbjct: 2   KKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGL 61

Query: 63  TISSI---HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGS 122
            I +I   +D+    VVQE  E SDG P TF PINPKKGVVRVSTDLNI+F  T +C  S
Sbjct: 62  AIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKS 121

Query: 123 TVWKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYG-NDYKFVYCPSVCKYCKVM 182
           TVWKLG FDRYLRQYFVTIGGTKGNPGRET ++WFKIEKYG  +YK VYCP VCKYCKVM
Sbjct: 122 TVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVM 181

Query: 183 CKDIGIFNFNGKRALGLSDAPFPVMFKKV 205
           CKDIGIF  NG R L LSD PFPV+FKKV
Sbjct: 182 CKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of Lag0024377 vs. ExPASy TrEMBL
Match: A0A1S3AT47 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 4.5e-80
Identity = 157/209 (75.12%), Postives = 168/209 (80.38%), Query Frame = 0


Query: 3   KKFVLLSFLFIA--IASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGS-GGGL 62
           KKF LLSFLFIA  IASSE+RFCRADASPD VLDTDGKKLR  + YYILPAF GS GGGL
Sbjct: 2   KKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGL 61

Query: 63  TISSI---HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGS 122
            I +I   +D+    VVQE  E SDG P TF PINPKKGVVRVSTDLNI+F  T +C  S
Sbjct: 62  AIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKS 121

Query: 123 TVWKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYG-NDYKFVYCPSVCKYCKVM 182
           TVWKLG FDRYLRQYFVTIGGTKGNPGRET ++WFKIEKYG  +YK VYCP VCKYCKVM
Sbjct: 122 TVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVM 181

Query: 183 CKDIGIFNFNGKRALGLSDAPFPVMFKKV 205
           CKDIGIF  NG R L LSD PFPV+FKKV
Sbjct: 182 CKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of Lag0024377 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.8e-79
Identity = 139/204 (68.14%), Postives = 169/204 (82.84%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           K F LL FLFI IASSEVRFCRADASPD VLDTDGKKLRAGD YYIL  +  + GGL+I 
Sbjct: 2   KNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  SI--HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWK 122
            I  +++    ++ E  +  DG+PATF+P+NPKKGVVRVSTDLNI+F+ + +C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVWK 121

Query: 123 LGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIG 182
           +GKFD+YL+QYFVT+GGTKGNPGRET+ +WFK+EK+G +YKFVYCP+VCKYCKVMCKD+G
Sbjct: 122 VGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDVG 181

Query: 183 IFNFNGKRALGLSDAPFPVMFKKV 205
           +F  NG+R   L+DAPFPVMFKKV
Sbjct: 182 LFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of Lag0024377 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.8e-79
Identity = 139/204 (68.14%), Postives = 169/204 (82.84%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           K F LL FLFI IASSEVRFCRADASPD VLDTDGKKLRAGD YYIL  +  + GGL+I 
Sbjct: 2   KNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  SI--HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWK 122
            I  +++    ++ E  +  DG+PATF+P+NPKKGVVRVSTDLNI+F+ + +C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTVWK 121

Query: 123 LGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIG 182
           +GKFD+YL+QYFVT+GGTKGNPGRET+ +WFK+EK+G +YKFVYCP+VCKYCKVMCKD+G
Sbjct: 122 VGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKDVG 181

Query: 183 IFNFNGKRALGLSDAPFPVMFKKV 205
           +F  NG+R   L+DAPFPVMFKKV
Sbjct: 182 LFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of Lag0024377 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.2e-77
Identity = 137/204 (67.16%), Postives = 166/204 (81.37%), Query Frame = 0


Query: 3   KKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS 62
           + F LL FLFI IASSEVRFCRADASPD VLDTDGKKLRAGD YYIL  +  + GGL+I 
Sbjct: 2   RNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSIG 61

Query: 63  SI--HDQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWK 122
            I  +++    ++ E  +   G+PATF+PINPKKGVVRVSTDLNIQF+   +C  STVWK
Sbjct: 62  GIYGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTVWK 121

Query: 123 LGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIG 182
           +GKFD YL+QYFVT+GG KGNPGRET+++WFK+EKYG +YK VYCP+VCKYCKV+CKD+G
Sbjct: 122 VGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKDVG 181

Query: 183 IFNFNGKRALGLSDAPFPVMFKKV 205
           +F  NG+R + L+DAPFPVMFKKV
Sbjct: 182 LFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of Lag0024377 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 178.3 bits (451), Expect = 6.1e-45
Identity = 86/199 (43.22%), Postives = 130/199 (65.33%), Query Frame = 0


Query: 6   VLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLTISSIH 65
           +L  FL +A+  S  R    +A+ + V D +GK L  G  YYILP   G GGGLT+S++ 
Sbjct: 4   LLYIFLLLAVFISH-RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLK 63

Query: 66  -DQLSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTLKCSGSTVWKLGKF 125
            +     V+Q+  E+S G+P  F+P + K   + VSTD+NI+F      S +++W+L  F
Sbjct: 64  TETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANF 123

Query: 126 DRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDIGIFNF 185
           D   +Q+F++  G +GNPG++T+D+WFKI+K+  DYK  +CP+VC +CKV+C+D+G+F  
Sbjct: 124 DETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQ 183

Query: 186 NGKRALGLSDAPFPVMFKK 204
           +GKR L LSD P  VMFK+
Sbjct: 184 DGKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Lag0024377 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-30
Identity = 82/207 (39.61%), Postives = 112/207 (54.11%), Query Frame = 0


Query: 1   MKKKFVLLSFLFIAIASSEVRFCRADASPDTVLDTDGKKLRAGDYYYILPAFHGSGGGLT 60
           M  KF L+  L   +AS+         +   V+D DG  +   + YY+LP   G GGGLT
Sbjct: 8   MNPKFYLVLALTAVLASN---------AYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLT 67

Query: 61  ISSIHDQ-LSNVVVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKL-TLKCSGSTV 120
           ++    Q     +VQE +E+ +G+P  F+    K   V  S +LNI+  +    C  ST 
Sbjct: 68  LAGRGGQPCPYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTY 127

Query: 121 WKLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGND-YKFVYCPSVCKYCKVMCK 180
           W++G+FD   +QYFV  G      G+++L S+FKIEK G D YKFV+CP  C      C 
Sbjct: 128 WRVGEFDHERKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCS 187

Query: 181 DIGIF-NFNGKRALGLSDAPFPVMFKK 204
           D+GIF +  G R L LSD PF VMFKK
Sbjct: 188 DVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Lag0024377 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 103.6 bits (257), Expect = 1.9e-22
Identity = 76/206 (36.89%), Postives = 113/206 (54.85%), Query Frame = 0


Query: 8   LSFLFIAIASS-EVRFCRADASP-DTVLDTDGKKLRAGDYYYILPAFHGSGGGLTIS--- 67
           LSF+ + + S+       ADA+P   VLD  G  +++   YYI+PA  G+GGGL  S   
Sbjct: 6   LSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRN 65

Query: 68  -SIHDQLSNV-VVQEPNELSDGVPATFAPINPKKGVVRVSTDLNIQFKLTL-KCSGSTVW 127
            S  D   N+ +VQ  +    G+P TF+P+N K   V++S  LN++F  T+  C  S VW
Sbjct: 66  LSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVW 125

Query: 128 KLGKFDRYLRQYFVTIGGTKGNPGRETLDSWFKIEKYGNDYKFVYCPSVCKYCKVMCKDI 187
           ++      LR+ FV+IGG KG       +SWF+I++ G+ YK +YCP       V C ++
Sbjct: 126 RI-DHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINV 185

Query: 188 GI-FNFNGKRALGLS-DAPFPVMFKK 204
            +  + +G R L LS D  F V F+K
Sbjct: 186 SLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Lag0024377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437071.1	9.4e-80	75.12	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]
XP_008437082.1	7.9e-79	68.14	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_022137472.1	7.4e-77	68.97	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_038895396.1	9.7e-77	68.29	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_008437058.1	4.2e-72	65.37	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]

BLAST of Lag0024377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	2.2e-47	49.04	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	8.6e-44	43.22	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	1.1e-35	44.22	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	2.7e-29	39.61	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	2.4e-22	33.82	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Lag0024377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BJ25	4.5e-80	75.12	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]
A0A1S3AT47	4.5e-80	75.12	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1	[more]
A0A5D3BJ02	3.8e-79	68.14	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	3.8e-79	68.14	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A0A0LT81	1.2e-77	67.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]

BLAST of Lag0024377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	6.1e-45	43.22	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	1.9e-30	39.61	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.9e-22	36.89	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 150..169 score: 26.96 coord: 68..88 score: 26.98 coord: 31..60 score: 44.58
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..204 e-value: 6.9E-60 score: 214.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..203 e-value: 3.3E-51 score: 173.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..204
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 32..203 e-value: 8.24203E-61 score: 185.624
None	No IPR available	GENE3D	2.80.10.50		coord: 26..204 e-value: 1.1E-66 score: 225.8
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..204
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0024377.1	Lag0024377.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity