Lag0024266 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024266
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein ASPARTIC PROTEASE IN GUARD CELL 1-like
Locationchr10: 1734876 .. 1736369 (+)
RNA-Seq ExpressionLag0024266
SyntenyLag0024266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCTGCGAATCTGCTGCCTCTGTTCGCCTTCGCCGTCTTCATTTTCTCTCTCTTTCCCTTCTCTCTCTGTCGTAGTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTCAAATTCGACCCCAAGGCTTTGACGTCTTTCCAGCAGCTGGAACAGCTTGTTCCGGCGAACTCTTCCTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACATAAGGACTACAAGAGCTTGGTCCTCTCCAGGCTCGAGCGGGACTCGTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTGCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTCGGCGTCGGACAACCGGCAAAGCCATTCTATATGGTTCTCGATACTGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTACTTCTCTTCCCTGCGAATCTCAGCAATGTCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTTACTGAAACGCTGTCGTTCGGTAACTCCGGGGCGATTAACAACGTCGCTCTCGGCTGCGGCCACGATAACGAAGGATTGTTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGAGCAATATCGCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCGTCTTCAAGTCTCGAGTTCAATTCCGCCGCACCGAGTGACTCAGTGACTGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGCACTCACCGGATTGAGCGTCGGCGGCCAGCTACTCTCAATTCCGCCTTCTCTGTTTCAAATGGACGATTCCGGCTACGGCGGCATCATCGTCGATTCAGGAACCGCCATAACTCGGCTGCAGACTCAGGCATACAACTCCCTTCGCGACGCCTTCGTGAGACTGACACCGTACTTGAAGAAGACGAACGGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCACAGTCGAGAGTCACCATTCCGACGGTGTCGTTTCACTTCGCCGGCGGAAAGTCCCTGCTGCTGCCGCCGAAGAACTACCTCATTCCGGTGGACTCCGCCGGAACCTTCTGCTTCGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGAAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGATTTGGCAAATTCGGTCGTAGGGTTTTCACCCAATAAATGTTAA

mRNA sequence

ATGGCGTCTGCGAATCTGCTGCCTCTGTTCGCCTTCGCCGTCTTCATTTTCTCTCTCTTTCCCTTCTCTCTCTGTCGTAGTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTCAAATTCGACCCCAAGGCTTTGACGTCTTTCCAGCAGCTGGAACAGCTTGTTCCGGCGAACTCTTCCTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACATAAGGACTACAAGAGCTTGGTCCTCTCCAGGCTCGAGCGGGACTCGTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTGCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTCGGCGTCGGACAACCGGCAAAGCCATTCTATATGGTTCTCGATACTGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTACTTCTCTTCCCTGCGAATCTCAGCAATGTCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTTACTGAAACGCTGTCGTTCGGTAACTCCGGGGCGATTAACAACGTCGCTCTCGGCTGCGGCCACGATAACGAAGGATTGTTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGAGCAATATCGCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCGTCTTCAAGTCTCGAGTTCAATTCCGCCGCACCGAGTGACTCAGTGACTGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGCACTCACCGGATTGAGCGTCGGCGGCCAGCTACTCTCAATTCCGCCTTCTCTGTTTCAAATGGACGATTCCGGCTACGGCGGCATCATCGTCGATTCAGGAACCGCCATAACTCGGCTGCAGACTCAGGCATACAACTCCCTTCGCGACGCCTTCGTGAGACTGACACCGTACTTGAAGAAGACGAACGGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCACAGTCGAGAGTCACCATTCCGACGGTGTCGTTTCACTTCGCCGGCGGAAAGTCCCTGCTGCTGCCGCCGAAGAACTACCTCATTCCGGTGGACTCCGCCGGAACCTTCTGCTTCGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGAAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGATTTGGCAAATTCGGTCGTAGGGTTTTCACCCAATAAATGTTAA

Coding sequence (CDS)

ATGGCGTCTGCGAATCTGCTGCCTCTGTTCGCCTTCGCCGTCTTCATTTTCTCTCTCTTTCCCTTCTCTCTCTGTCGTAGTTCGCCGGAGCTTTCTCACTCCTCCTCCTCCTCCTCCTCCTCCGCTTTTCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTCAAATTCGACCCCAAGGCTTTGACGTCTTTCCAGCAGCTGGAACAGCTTGTTCCGGCGAACTCTTCCTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACATAAGGACTACAAGAGCTTGGTCCTCTCCAGGCTCGAGCGGGACTCGTCTAGAGTTAAATCGCTCAACGATAGGCTTGAATTTGCCCTCAGTGACTTGAAGAGGTCGGATCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCTGGCTCCAGTCAGGGGAGTGGCGAGTACTTCTCCCGCGTCGGCGTCGGACAACCGGCAAAGCCATTCTATATGGTTCTCGATACTGGCAGTGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGTTACCAGCAAACCGACCCGATTTTTGACCCGAGAACTTCGTCTTCTTTTACTTCTCTTCCCTGCGAATCTCAGCAATGTCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCGAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTTACTGAAACGCTGTCGTTCGGTAACTCCGGGGCGATTAACAACGTCGCTCTCGGCTGCGGCCACGATAACGAAGGATTGTTCGTCGGATCCGCCGGATTGCTCGGACTCGGCGGCGGAGCAATATCGCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTCGACCGCGACTCTGGTTCGTCTTCAAGTCTCGAGTTCAATTCCGCCGCACCGAGTGACTCAGTGACTGCTCCATTACTCAGAAGCGGAAGAGTCGATACATTCTACTACGTCGCACTCACCGGATTGAGCGTCGGCGGCCAGCTACTCTCAATTCCGCCTTCTCTGTTTCAAATGGACGATTCCGGCTACGGCGGCATCATCGTCGATTCAGGAACCGCCATAACTCGGCTGCAGACTCAGGCATACAACTCCCTTCGCGACGCCTTCGTGAGACTGACACCGTACTTGAAGAAGACGAACGGCTTCGCATTATTCGACACATGTTACGATCTGTCGTCACAGTCGAGAGTCACCATTCCGACGGTGTCGTTTCACTTCGCCGGCGGAAAGTCCCTGCTGCTGCCGCCGAAGAACTACCTCATTCCGGTGGACTCCGCCGGAACCTTCTGCTTCGCGTTCGCCCCAACGACGTCGTCTTTGTCCATCATAGGAAACGTTCAGCAGCAAGGGACACGTGTCAACTTCGATTTGGCAAATTCGGTCGTAGGGTTTTCACCCAATAAATGTTAA

Protein sequence

MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKASSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVGFSPNKC
Homology
BLAST of Lag0024266 vs. NCBI nr
Match: XP_022137431.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Momordica charantia])

HSP 1 Score: 891.7 bits (2303), Expect = 2.8e-255
Identity = 457/487 (93.84%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 12  FAVFIFSLF-PFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALTSFQQL 71
           F +FIFSLF PF+ CRSSPELS      SSSA LDVSASLKQADQVLKFDPK L SFQQ 
Sbjct: 6   FTIFIFSLFLPFTSCRSSPELSR-----SSSALLDVSASLKQADQVLKFDPKGLMSFQQQ 65

Query: 72  EQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLK 131
           EQLVPAN SSSFSLQLHPRDALRNAGHKDYKSLVLSRL+RDSSRVKSLNDRL+FALSDLK
Sbjct: 66  EQLVPAN-SSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLK 125

Query: 132 RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 191
           RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ
Sbjct: 126 RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 185

Query: 192 PCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF 251
           PCTDCYQQ+DPIFDPRTSSSF SLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF
Sbjct: 186 PCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF 245

Query: 252 VTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKASSFSYCLVDR 311
           VTETLSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMKASSFSYCLVDR
Sbjct: 246 VTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDR 305

Query: 312 DSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMDDSG 371
           DSGSSSSL+FNS APSDSVTAPLLRSGRVDTFYYV LTGLSVGGQLLSIPPSLFQMDDSG
Sbjct: 306 DSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSG 365

Query: 372 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTV 431
           YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYL+KTNGFALFDTCYDLSSQSRVTIPTV
Sbjct: 366 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTV 425

Query: 432 SFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 491
           SFHF+GGKSL+LPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS+V
Sbjct: 426 SFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLV 485

Query: 492 GFSPNKC 498
           GFSPNKC
Sbjct: 486 GFSPNKC 486

BLAST of Lag0024266 vs. NCBI nr
Match: KAG6584369.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019953.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 886.7 bits (2290), Expect = 9.1e-254
Identity = 449/498 (90.16%), Postives = 477/498 (95.78%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSH-SSSSSSSSAFLDVSASLKQADQVLKF 60
           MA+ANLLPLF+FA+F FSL PF+LCR+SPELSH SSSSSSSSAFLDVSASLKQA+QVLKF
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSHSSSSSSSSSAFLDVSASLKQANQVLKF 60

Query: 61  DPKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLN 120
           DPKAL SFQQ +++VP NSSSSFSLQLH RDAL+NAGHKDYKSLVLSRL+RDSSRVKSLN
Sbjct: 61  DPKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLN 120

Query: 121 DRLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLD 180
           DRLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLD
Sbjct: 121 DRLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLD 180

Query: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVS 240
           TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SLPCES QCQALE SGCRAAKCLYQV+
Sbjct: 181 TGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVA 240

Query: 241 YGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMK 300
           YGDGSFTVGEFVTE+LSFGNSG I+NVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMK
Sbjct: 241 YGDGSFTVGEFVTESLSFGNSGTISNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK 300

Query: 301 ASSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSI 360
           ASSFSYCLVDRDS SSS+LEFNSAAPSDSV APLLRSGRVDTFYYV L GLSVGGQLLSI
Sbjct: 301 ASSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSI 360

Query: 361 PPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDL 420
           PPSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNGFALFDTCYDL
Sbjct: 361 PPSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDL 420

Query: 421 SSQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGT 480
           SSQSRVTIPT+SF FAGGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGT
Sbjct: 421 SSQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGT 480

Query: 481 RVNFDLANSVVGFSPNKC 498
           RV+FDLANS+VGFSPNKC
Sbjct: 481 RVHFDLANSLVGFSPNKC 498

BLAST of Lag0024266 vs. NCBI nr
Match: XP_023519500.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.3 bits (2276), Expect = 3.8e-252
Identity = 445/497 (89.54%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA+ANLLPLF+FA+F FSL PF+LCR+SPELSH  SSSSSSAFLDVSASLKQA+QVLKFD
Sbjct: 1   MATANLLPLFSFAIFFFSLLPFTLCRTSPELSH--SSSSSSAFLDVSASLKQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           PKAL SFQQ +++VP NSSSSFSLQLH RDAL+NAGHKDYKSLVLSRL+RDSSRVKSLND
Sbjct: 61  PKALLSFQQQDEVVPGNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDT
Sbjct: 121 RLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SLPCES QCQALE SGCRAAKCLYQV+Y
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTE+LSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMKA
Sbjct: 241 GDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS+LEFNSAAPSDSV APLL+SGRVDTFYYV L GLSVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLKSGRVDTFYYVGLVGLSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           PSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNGFALFDTCYDLS
Sbjct: 361 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPT+SF FAGG SL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTLSFQFAGGNSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V+FDLANS+VGFSPNKC
Sbjct: 481 VHFDLANSLVGFSPNKC 495

BLAST of Lag0024266 vs. NCBI nr
Match: XP_022924034.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 879.4 bits (2271), Expect = 1.5e-251
Identity = 445/497 (89.54%), Postives = 473/497 (95.17%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA+ANLLPLF+FA F FSL PF+LCR+SPELSH   SSSSSAFLDVSASLKQA+QVLKFD
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH---SSSSSAFLDVSASLKQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           PKAL SFQQ +++VP NSSSSFSLQLH RDAL+NAGH+DYKSLVLSRL+RDSSRVKSLND
Sbjct: 61  PKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDT
Sbjct: 121 RLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SLPCES QCQALE SGCRAAKCLYQV+Y
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTE+LSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMKA
Sbjct: 241 GDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS+LEFNSAAPSDSV APLLRSGRVDTFYYV L GLSVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           PSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNGFALFDTCYDLS
Sbjct: 361 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPT+SF FAGGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V+FDLANS+VGFSPNKC
Sbjct: 481 VHFDLANSLVGFSPNKC 494

BLAST of Lag0024266 vs. NCBI nr
Match: XP_023000890.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 874.0 bits (2257), Expect = 6.1e-250
Identity = 443/497 (89.13%), Postives = 471/497 (94.77%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA+ANL PLF+FA+F FSL PF+LCR+SPELSH  SSSSSSAFLDVSASLKQA+QVLKFD
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSH--SSSSSSAFLDVSASLKQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           PKAL SFQQ  ++VPANSSSSFSLQLH RDAL+NAGHKDYKSLVLSRL+RDSSRVKSLND
Sbjct: 61  PKALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDT
Sbjct: 121 RLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SL CES QCQALE SGCRAAKCLYQV+Y
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTE+LSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SL SQMKA
Sbjct: 241 GDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS+LEFNSAAPSD+V APLLRSGRVDTFYYV L GLSVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           PSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNG ALFDTCYDLS
Sbjct: 361 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPT+SF FAGGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V+FDLANS++GFSPNKC
Sbjct: 481 VHFDLANSLLGFSPNKC 495

BLAST of Lag0024266 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.4e-167
Identity = 306/498 (61.45%), Postives = 375/498 (75.30%), Query Frame = 0

Query: 9   LFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPK----AL 68
           L   AV   SLF      ++   S S S+   +  LDV +SL+Q   +L  DP       
Sbjct: 7   LSLLAVVTLSLF----LTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTT 66

Query: 69  TSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEF 128
           T  + L   V  NSSS  SL+LH RD    + HKDYKSL LSRLERDSSRV  +  ++ F
Sbjct: 67  TKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRF 126

Query: 129 ALSDLKRSDLQPL---KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTG 188
           A+  + RSDL+P+    T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTG
Sbjct: 127 AVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTG 186

Query: 189 SDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYG 248
           SD+NW+QC+PC DCYQQ+DP+F+P +SS++ SL C + QC  LE S CR+ KCLYQVSYG
Sbjct: 187 SDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 246

Query: 249 DGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKAS 308
           DGSFTVGE  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG +S+T+QMKA+
Sbjct: 247 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 306

Query: 309 SFSYCLVDRDSGSSSSLEFNSA-APSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 368
           SFSYCLVDRDSG SSSL+FNS        TAPLLR+ ++DTFYYV L+G SVGG+ + +P
Sbjct: 307 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 366

Query: 369 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-TNGFALFDTCYDL 428
            ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++  +LFDTCYD 
Sbjct: 367 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 426

Query: 429 SSQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGT 488
           SS S V +PTV+FHF GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGT
Sbjct: 427 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 486

Query: 489 RVNFDLANSVVGFSPNKC 498
           R+ +DL+ +V+G S NKC
Sbjct: 487 RITYDLSKNVIGLSGNKC 500

BLAST of Lag0024266 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.2e-110
Identity = 246/487 (50.51%), Postives = 305/487 (62.63%), Query Frame = 0

Query: 22  FSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALTSF---QQLEQLVPANS 81
           FSLC     L   SS  S       S SL  A  V  F P + +      + E    + S
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPV-SFQPDSDSESLLESEFESGSDSES 69

Query: 82  SSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPLK 141
           SSS +L L   DAL  + +K    L  SRL+RDS RVKS+      A     R+     +
Sbjct: 70  SSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI---ATLAAQIPGRNVTHAPR 129

Query: 142 TEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQ 201
               P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY Q
Sbjct: 130 ----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 189

Query: 202 TDPIFDPRTSSSFTSLPCESQQCQALEMSGC--RAAKCLYQVSYGDGSFTVGEFVTETLS 261
           +DPIFDPR S ++ ++PC S  C+ L+ +GC  R   CLYQVSYGDGSFTVG+F TETL+
Sbjct: 190 SDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLT 249

Query: 262 FGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISL---TSQMKASSFSYCLVDRDSG 321
           F     +  VALGCGHDNEGLFVG+AGLLGLG G +S    T       FSYCLVDR + 
Sbjct: 250 F-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSAS 309

Query: 322 SS-SSLEFNSAAPSDSVT-APLLRSGRVDTFYYVALTGLSVGG-QLLSIPPSLFQMDDSG 381
           S  SS+ F +AA S      PLL + ++DTFYYV L G+SVGG ++  +  SLF++D  G
Sbjct: 310 SKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIG 369

Query: 382 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTV 441
            GG+I+DSGT++TRL   AY ++RDAF      LK+   F+LFDTC+DLS+ + V +PTV
Sbjct: 370 NGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTV 429

Query: 442 SFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 498
             HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S V
Sbjct: 430 VLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRV 484

BLAST of Lag0024266 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.2e-107
Identity = 196/424 (46.23%), Postives = 277/424 (65.33%), Query Frame = 0

Query: 78  SSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPL 137
           SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 138 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 197
            +     D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 198 QTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 257
           Q+DP+FDP  S S+T + C S  C  +E SGC +  C Y+V YGDGS+T G    ETL+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 258 GNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMK---ASSFSYCLVDRDSGS 317
             +  + NVA+GCGH N G+F+G+AGLLG+GGG++S   Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 318 SSSLEF-NSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMDDSGYGG 377
           + SL F   A P  +   PL+R+ R  +FYYV L GL VGG  + +P  +F + ++G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 378 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFH 437
           +++D+GTA+TRL T AY + RD F   T  L + +G ++FDTCYDLS    V +PTVSF+
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 438 FAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVGFS 497
           F  G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  VGF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

BLAST of Lag0024266 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.8e-74
Identity = 155/347 (44.67%), Postives = 207/347 (59.65%), Query Frame = 0

Query: 157 GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPC 216
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ Q+ PIF+P+ SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 217 ESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEG 276
            SQ CQAL    C    C Y   YGDGS T G   TETL+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 277 LFVGS-AGLLGLGGGAISLTSQMKASSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAP--- 336
              G+ AGL+G+G G +SL SQ+  + FSYC+    S + S+L   S A S +  +P   
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 337 LLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMD-DSGYGGIIVDSGTAITRLQTQAYN 396
           L++S ++ TFYY+ L GLSVG   L I PS F ++ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 397 SLRDAFVRLTPYLKKTNGFALFDTCYDL-SSQSRVTIPTVSFHFAGGKSLLLPPKNYLIP 456
           S+R  F+            + FD C+   S  S + IPT   HF GG  L LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 457 VDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVGFSPNKC 498
             S G  C A   ++  +SI GN+QQQ   V +D  NSVV F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Lag0024266 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 9.7e-73
Identity = 161/404 (39.85%), Postives = 224/404 (55.45%), Query Frame = 0

Query: 103 LVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYF 162
           L+   ++R   R++S+N  L+ +                    + TP+ +    G GEY 
Sbjct: 60  LIKRAIKRGERRMRSINAMLQSS------------------SGIETPVYA----GDGEYL 119

Query: 163 SRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQ 222
             V +G P   F  ++DTGSD+ W QC+PCT C+ Q  PIF+P+ SSSF++LPCESQ CQ
Sbjct: 120 MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 179

Query: 223 ALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGS- 282
            L    C   +C Y   YGDGS T G   TET +F  S ++ N+A GCG DN+G   G+ 
Sbjct: 180 DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNG 239

Query: 283 AGLLGLGGGAISLTSQMKASSFSYCLVDRDSGSSSSLEFNSAA---PSDSVTAPLLRSGR 342
           AGL+G+G G +SL SQ+    FSYC+    S S S+L   SAA   P  S +  L+ S  
Sbjct: 240 AGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSL 299

Query: 343 VDTFYYVALTGLSVGGQLLSIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAF- 402
             T+YY+ L G++VGG  L IP S FQ+ D G GG+I+DSGT +T L   AYN++  AF 
Sbjct: 300 NPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFT 359

Query: 403 --VRLTPYLKKTNGFALFDTCYDLSSQ-SRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSA 462
             + L    + ++G +   TC+   S  S V +P +S  F GG  L L  +N LI   + 
Sbjct: 360 DQINLPTVDESSSGLS---TCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAE 419

Query: 463 GTFCFAFAPTTS-SLSIIGNVQQQGTRVNFDLANSVVGFSPNKC 498
           G  C A   ++   +SI GN+QQQ T+V +DL N  V F P +C
Sbjct: 420 GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Lag0024266 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 457/487 (93.84%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 12  FAVFIFSLF-PFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALTSFQQL 71
           F +FIFSLF PF+ CRSSPELS      SSSA LDVSASLKQADQVLKFDPK L SFQQ 
Sbjct: 6   FTIFIFSLFLPFTSCRSSPELSR-----SSSALLDVSASLKQADQVLKFDPKGLMSFQQQ 65

Query: 72  EQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLK 131
           EQLVPAN SSSFSLQLHPRDALRNAGHKDYKSLVLSRL+RDSSRVKSLNDRL+FALSDLK
Sbjct: 66  EQLVPAN-SSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLK 125

Query: 132 RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 191
           RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ
Sbjct: 126 RSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQ 185

Query: 192 PCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF 251
           PCTDCYQQ+DPIFDPRTSSSF SLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF
Sbjct: 186 PCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEF 245

Query: 252 VTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKASSFSYCLVDR 311
           VTETLSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMKASSFSYCLVDR
Sbjct: 246 VTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDR 305

Query: 312 DSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMDDSG 371
           DSGSSSSL+FNS APSDSVTAPLLRSGRVDTFYYV LTGLSVGGQLLSIPPSLFQMDDSG
Sbjct: 306 DSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSG 365

Query: 372 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTV 431
           YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYL+KTNGFALFDTCYDLSSQSRVTIPTV
Sbjct: 366 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTV 425

Query: 432 SFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 491
           SFHF+GGKSL+LPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS+V
Sbjct: 426 SFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLV 485

Query: 492 GFSPNKC 498
           GFSPNKC
Sbjct: 486 GFSPNKC 486

BLAST of Lag0024266 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.1e-252
Identity = 445/497 (89.54%), Postives = 473/497 (95.17%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA+ANLLPLF+FA F FSL PF+LCR+SPELSH   SSSSSAFLDVSASLKQA+QVLKFD
Sbjct: 1   MATANLLPLFSFASFFFSLLPFTLCRTSPELSH---SSSSSAFLDVSASLKQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           PKAL SFQQ +++VP NSSSSFSLQLH RDAL+NAGH+DYKSLVLSRL+RDSSRVKSLND
Sbjct: 61  PKALLSFQQQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDT
Sbjct: 121 RLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SLPCES QCQALE SGCRAAKCLYQV+Y
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTE+LSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SLTSQMKA
Sbjct: 241 GDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS+LEFNSAAPSDSV APLLRSGRVDTFYYV L GLSVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           PSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNGFALFDTCYDLS
Sbjct: 361 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPT+SF FAGGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V+FDLANS+VGFSPNKC
Sbjct: 481 VHFDLANSLVGFSPNKC 494

BLAST of Lag0024266 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.0e-250
Identity = 443/497 (89.13%), Postives = 471/497 (94.77%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA+ANL PLF+FA+F FSL PF+LCR+SPELSH  SSSSSSAFLDVSASLKQA+QVLKFD
Sbjct: 1   MATANLQPLFSFAIFFFSLLPFTLCRTSPELSH--SSSSSSAFLDVSASLKQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           PKAL SFQQ  ++VPANSSSSFSLQLH RDAL+NAGHKDYKSLVLSRL+RDSSRVKSLND
Sbjct: 61  PKALLSFQQQNEVVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLND 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEF+LSDLKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDT
Sbjct: 121 RLEFSLSDLKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPRTSSSF SL CES QCQALE SGCRAAKCLYQV+Y
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTE+LSFGNSG INNVALGCGHDNEGLFVGSAGLLGLGGG++SL SQMKA
Sbjct: 241 GDGSFTVGEFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS+LEFNSAAPSD+V APLLRSGRVDTFYYV L GLSVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           PSLFQMDD+GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYL++TNG ALFDTCYDLS
Sbjct: 361 PSLFQMDDAGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPT+SF FAGGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V+FDLANS++GFSPNKC
Sbjct: 481 VHFDLANSLLGFSPNKC 495

BLAST of Lag0024266 vs. ExPASy TrEMBL
Match: A0A6J1GS20 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456998 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.3e-241
Identity = 441/503 (87.67%), Postives = 459/503 (91.25%), Query Frame = 0

Query: 1   MASA--NLLPLFAFAVFIF---SLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQ 60
           MASA  N LP F FA+F+F   SLFPF+L         SSSSSSSSA LDVS SLKQA Q
Sbjct: 1   MASARPNQLPFFPFAIFLFLFLSLFPFTL---------SSSSSSSSASLDVSTSLKQAHQ 60

Query: 61  VLKFDPKALTSFQQLEQLVPAN-SSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSR 120
           VLKF PK LTSF Q E LVPAN SSSSFSLQLHPRDALRNAGH DY+SLVLSRL+RDSSR
Sbjct: 61  VLKFGPKILTSFHQQELLVPANSSSSSFSLQLHPRDALRNAGHTDYRSLVLSRLDRDSSR 120

Query: 121 VKSLNDRLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPF 180
           VKSLNDRLEF  +DL RSDLQPL T+ILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPF
Sbjct: 121 VKSLNDRLEFGSTDLTRSDLQPLNTDILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPF 180

Query: 181 YMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKC 240
           YMVLDTGSDINWLQCQPCTDCYQQTDPIFDP TSSSFTSLPCES  CQALEMSGCRAAKC
Sbjct: 181 YMVLDTGSDINWLQCQPCTDCYQQTDPIFDPTTSSSFTSLPCESPHCQALEMSGCRAAKC 240

Query: 241 LYQVSYGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISL 300
           LYQVSYGDGSFTVG+FVTETLSFGNSG INNVALGCGHDNEGLF+GSAGLLGLGGG++SL
Sbjct: 241 LYQVSYGDGSFTVGQFVTETLSFGNSGVINNVALGCGHDNEGLFIGSAGLLGLGGGSLSL 300

Query: 301 TSQMKASSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGG 360
            SQMKASSFSYCLVDRDSGS+S+LEFNSAAP+DSV A LLRSGRV+TFYYVALTGLSVGG
Sbjct: 301 NSQMKASSFSYCLVDRDSGSASTLEFNSAAPNDSVNAQLLRSGRVNTFYYVALTGLSVGG 360

Query: 361 QLLSIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFD 420
             LSIPPSLFQ+DDSG+GGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK N FALFD
Sbjct: 361 HPLSIPPSLFQLDDSGHGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKANSFALFD 420

Query: 421 TCYDLSSQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNV 480
           TCYDLSSQSRVTIPTVSFHF+GGKSL LPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNV
Sbjct: 421 TCYDLSSQSRVTIPTVSFHFSGGKSLQLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNV 480

Query: 481 QQQGTRVNFDLANSVVGFSPNKC 498
           QQQGTRVNFDLANSVV FSPNKC
Sbjct: 481 QQQGTRVNFDLANSVVAFSPNKC 494

BLAST of Lag0024266 vs. ExPASy TrEMBL
Match: A0A5D3BI72 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001860 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.1e-240
Identity = 432/497 (86.92%), Postives = 460/497 (92.56%), Query Frame = 0

Query: 1   MASANLLPLFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFD 60
           MA++NLL LF   +F FSLFPF+L RSS  LS  SS+S     LDVSASL+QA+QVLKFD
Sbjct: 1   MATSNLLTLF---LFFFSLFPFALSRSSSHLSPHSSAS-----LDVSASLQQANQVLKFD 60

Query: 61  PKALTSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLND 120
           P A  SFQQ  +LVP+NSS SFSLQLHPRD+L NAGHKDYKSLVLSRL RDSSRVKS+ D
Sbjct: 61  PTASISFQQQVRLVPSNSSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYD 120

Query: 121 RLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDT 180
           RLEFALS+LKRSDL+PLKTEILPEDLSTPIVSG+SQGSGEYFSRVGVGQPAKPFYMVLDT
Sbjct: 121 RLEFALSELKRSDLEPLKTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDT 180

Query: 181 GSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSY 240
           GSDINWLQCQPCTDCYQQTDPIFDPR+SSSF SLPCESQQCQALE SGCRA+KCLYQVSY
Sbjct: 181 GSDINWLQCQPCTDCYQQTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSY 240

Query: 241 GDGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKA 300
           GDGSFTVGEFVTETL+FGNSG I NVA+GCGHDNEGLFVGSAGLLGLGGG++SLTSQMKA
Sbjct: 241 GDGSFTVGEFVTETLTFGNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKA 300

Query: 301 SSFSYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 360
           SSFSYCLVDRDS SSS LEFNSAAP+DSV APLL+SGRVDTFYYV LTG+SVGGQLLSIP
Sbjct: 301 SSFSYCLVDRDSSSSSDLEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIP 360

Query: 361 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLS 420
           P+LFQMDDSGYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLKKTNGFALFDTCYDLS
Sbjct: 361 PNLFQMDDSGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLS 420

Query: 421 SQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTR 480
           SQSRVTIPTVSF FAGGKSL LPPKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTR
Sbjct: 421 SQSRVTIPTVSFEFAGGKSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTR 480

Query: 481 VNFDLANSVVGFSPNKC 498
           V++DLANSVVGFSP+KC
Sbjct: 481 VHYDLANSVVGFSPHKC 489

BLAST of Lag0024266 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 590.9 bits (1522), Expect = 9.6e-169
Identity = 306/498 (61.45%), Postives = 375/498 (75.30%), Query Frame = 0

Query: 9   LFAFAVFIFSLFPFSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPK----AL 68
           L   AV   SLF      ++   S S S+   +  LDV +SL+Q   +L  DP       
Sbjct: 7   LSLLAVVTLSLF----LTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTT 66

Query: 69  TSFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEF 128
           T  + L   V  NSSS  SL+LH RD    + HKDYKSL LSRLERDSSRV  +  ++ F
Sbjct: 67  TKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRF 126

Query: 129 ALSDLKRSDLQPL---KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTG 188
           A+  + RSDL+P+    T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTG
Sbjct: 127 AVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTG 186

Query: 189 SDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYG 248
           SD+NW+QC+PC DCYQQ+DP+F+P +SS++ SL C + QC  LE S CR+ KCLYQVSYG
Sbjct: 187 SDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYG 246

Query: 249 DGSFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKAS 308
           DGSFTVGE  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG +S+T+QMKA+
Sbjct: 247 DGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT 306

Query: 309 SFSYCLVDRDSGSSSSLEFNSA-APSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIP 368
           SFSYCLVDRDSG SSSL+FNS        TAPLLR+ ++DTFYYV L+G SVGG+ + +P
Sbjct: 307 SFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLP 366

Query: 369 PSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKK-TNGFALFDTCYDL 428
            ++F +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  LKK ++  +LFDTCYD 
Sbjct: 367 DAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDF 426

Query: 429 SSQSRVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGT 488
           SS S V +PTV+FHF GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGT
Sbjct: 427 SSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGT 486

Query: 489 RVNFDLANSVVGFSPNKC 498
           R+ +DL+ +V+G S NKC
Sbjct: 487 RITYDLSKNVIGLSGNKC 500

BLAST of Lag0024266 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 565.8 bits (1457), Expect = 3.3e-161
Identity = 290/494 (58.70%), Postives = 378/494 (76.52%), Query Frame = 0

Query: 8   PLFAFAVFIFSLFPFS--LCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALT 67
           P ++F  FIF L   S    R  PE     +S+++++ L+V+ S+ +      F      
Sbjct: 3   PNYSFFFFIFFLTSHSSVFSRILPE-----TSTTTTSILNVADSIHRTKYTSSFRLN--- 62

Query: 68  SFQQLEQLVPANSSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFA 127
             QQ EQ    ++SSSFSLQLH R ++R   H DYKSL L+RL RD++RVKSL  RL+ A
Sbjct: 63  --QQEEQ--THSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLA 122

Query: 128 LSDLKRSDLQPLKTEILPE--DLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSD 187
           ++++ ++DL+P+ T    E  D+  P++SG++QGSGEYF+RVG+G+PA+  YMVLDTGSD
Sbjct: 123 INNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSD 182

Query: 188 INWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDG 247
           +NWLQC PC DCY QT+PIF+P +SSS+  L C++ QC ALE+S CR A CLY+VSYGDG
Sbjct: 183 VNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDG 242

Query: 248 SFTVGEFVTETLSFGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMKASSF 307
           S+TVG+F TETL+ G S  + NVA+GCGH NEGLFVG+AGLLGLGGG ++L SQ+  +SF
Sbjct: 243 SYTVGDFATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSF 302

Query: 308 SYCLVDRDSGSSSSLEFNSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSL 367
           SYCLVDRDS S+S+++F ++   D+V APLLR+ ++DTFYY+ LTG+SVGG+LL IP S 
Sbjct: 303 SYCLVDRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSS 362

Query: 368 FQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQS 427
           F+MD+SG GGII+DSGTA+TRLQT+ YNSLRD+FV+ T  L+K  G A+FDTCY+LS+++
Sbjct: 363 FEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKT 422

Query: 428 RVTIPTVSFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNF 487
            V +PTV+FHF GGK L LP KNY+IPVDS GTFC AFAPT SSL+IIGNVQQQGTRV F
Sbjct: 423 TVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTF 482

Query: 488 DLANSVVGFSPNKC 498
           DLANS++GFS NKC
Sbjct: 483 DLANSLIGFSSNKC 483

BLAST of Lag0024266 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 401.7 bits (1031), Expect = 8.3e-112
Identity = 246/487 (50.51%), Postives = 305/487 (62.63%), Query Frame = 0

Query: 22  FSLCRSSPELSHSSSSSSSSAFLDVSASLKQADQVLKFDPKALTSF---QQLEQLVPANS 81
           FSLC     L   SS  S       S SL  A  V  F P + +      + E    + S
Sbjct: 10  FSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPV-SFQPDSDSESLLESEFESGSDSES 69

Query: 82  SSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPLK 141
           SSS +L L   DAL  + +K    L  SRL+RDS RVKS+      A     R+     +
Sbjct: 70  SSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI---ATLAAQIPGRNVTHAPR 129

Query: 142 TEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQ 201
               P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  CY Q
Sbjct: 130 ----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQ 189

Query: 202 TDPIFDPRTSSSFTSLPCESQQCQALEMSGC--RAAKCLYQVSYGDGSFTVGEFVTETLS 261
           +DPIFDPR S ++ ++PC S  C+ L+ +GC  R   CLYQVSYGDGSFTVG+F TETL+
Sbjct: 190 SDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLT 249

Query: 262 FGNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISL---TSQMKASSFSYCLVDRDSG 321
           F     +  VALGCGHDNEGLFVG+AGLLGLG G +S    T       FSYCLVDR + 
Sbjct: 250 F-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSAS 309

Query: 322 SS-SSLEFNSAAPSDSVT-APLLRSGRVDTFYYVALTGLSVGG-QLLSIPPSLFQMDDSG 381
           S  SS+ F +AA S      PLL + ++DTFYYV L G+SVGG ++  +  SLF++D  G
Sbjct: 310 SKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIG 369

Query: 382 YGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTV 441
            GG+I+DSGT++TRL   AY ++RDAF      LK+   F+LFDTC+DLS+ + V +PTV
Sbjct: 370 NGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTV 429

Query: 442 SFHFAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVV 498
             HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA+S V
Sbjct: 430 VLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRV 484

BLAST of Lag0024266 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.7e-110
Identity = 233/473 (49.26%), Postives = 299/473 (63.21%), Query Frame = 0

Query: 43  FLDVSASLKQADQVLKFDPKALT-SFQQLEQLVP---ANSSSSFSLQLHPRDALRNAGHK 102
           F   SAS +    V+   P + T S+ + E L     + S++S S+ L   DAL +    
Sbjct: 18  FFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLSHVDALSSFSDA 77

Query: 103 DYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGS 162
               L   RL+RDS RVKS+      +L+ +        +T       S  ++SG SQGS
Sbjct: 78  SPADLFNLRLQRDSLRVKSIT-----SLAAVSTGRNATKRTPRTAGGFSGAVISGLSQGS 137

Query: 163 GEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQTDPIFDPRTSSSFTSLPCES 222
           GEYF R+GVG PA   YMVLDTGSD+ WLQC PC  CY QTD IFDP+ S +F ++PC S
Sbjct: 138 GEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCGS 197

Query: 223 QQCQAL-EMSGC---RAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGAINNVALGCGHDN 282
           + C+ L + S C   R+  CLYQVSYGDGSFT G+F TETL+F +   +++V LGCGHDN
Sbjct: 198 RLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTF-HGARVDHVPLGCGHDN 257

Query: 283 EGLFVGSAGLLGLGGGAISLTSQMK---ASSFSYCLVDRDSGSSSS------LEFNSAAP 342
           EGLFVG+AGLLGLG G +S  SQ K      FSYCLVDR S  SSS      +  N+A P
Sbjct: 258 EGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVP 317

Query: 343 SDSVTAPLLRSGRVDTFYYVALTGLSVGG-QLLSIPPSLFQMDDSGYGGIIVDSGTAITR 402
             SV  PLL + ++DTFYY+ L G+SVGG ++  +  S F++D +G GG+I+DSGT++TR
Sbjct: 318 KTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTR 377

Query: 403 LQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFHFAGGKSLLLPP 462
           L   AY +LRDAF      LK+   ++LFDTC+DLS  + V +PTV FHF GG+ + LP 
Sbjct: 378 LTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGE-VSLPA 437

Query: 463 KNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVGFSPNKC 498
            NYLIPV++ G FCFAFA T  SLSIIGN+QQQG RV +DL  S VGF    C
Sbjct: 438 SNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of Lag0024266 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 391.7 bits (1005), Expect = 8.6e-109
Identity = 196/424 (46.23%), Postives = 277/424 (65.33%), Query Frame = 0

Query: 78  SSSSFSLQLHPRDALRNAGHKDYKSLVLSRLERDSSRVKSLNDRLEFALSDLKRSDLQPL 137
           SSS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 138 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 197
            +     D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 198 QTDPIFDPRTSSSFTSLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 257
           Q+DP+FDP  S S+T + C S  C  +E SGC +  C Y+V YGDGS+T G    ETL+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 258 GNSGAINNVALGCGHDNEGLFVGSAGLLGLGGGAISLTSQMK---ASSFSYCLVDRDSGS 317
             +  + NVA+GCGH N G+F+G+AGLLG+GGG++S   Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 318 SSSLEF-NSAAPSDSVTAPLLRSGRVDTFYYVALTGLSVGGQLLSIPPSLFQMDDSGYGG 377
           + SL F   A P  +   PL+R+ R  +FYYV L GL VGG  + +P  +F + ++G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 378 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFH 437
           +++D+GTA+TRL T AY + RD F   T  L + +G ++FDTCYDLS    V +PTVSF+
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 438 FAGGKSLLLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSVVGFS 497
           F  G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  VGF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137431.12.8e-25593.84protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Momordica charantia][more]
KAG6584369.19.1e-25490.16Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp... [more]
XP_023519500.13.8e-25289.54protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo][more]
XP_022924034.11.5e-25189.54protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata][more]
XP_023000890.16.1e-25089.13protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LS401.4e-16761.45Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ31.2e-11050.51Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LHE31.2e-10746.23Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q766C31.8e-7444.67Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C29.7e-7339.85Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1C6K91.4e-25593.84protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1E8157.1e-25289.54protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KJJ83.0e-25089.13protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GS207.3e-24187.67protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5D3BI722.1e-24086.92Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT3G18490.19.6e-16961.45Eukaryotic aspartyl protease family protein [more]
AT1G25510.13.3e-16158.70Eukaryotic aspartyl protease family protein [more]
AT1G01300.18.3e-11250.51Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.7e-11049.26Eukaryotic aspartyl protease family protein [more]
AT3G20015.18.6e-10946.23Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 137..319
e-value: 2.1E-50
score: 173.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 320..497
e-value: 7.3E-52
score: 177.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 155..497
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 161..320
e-value: 5.4E-48
score: 163.5
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 342..493
e-value: 4.3E-37
score: 127.4
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 26..497
NoneNo IPR availablePANTHERPTHR13683:SF274PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1coord: 26..497
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 176..187
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 161..493
score: 46.890614

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024266.1Lag0024266.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity