Lag0024264 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024264
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAbhydro_lipase domain-containing protein
Locationchr10: 1721094 .. 1725908 (-)
RNA-Seq ExpressionLag0024264
SyntenyLag0024264
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCAACGCTTTGTCGACGATGTTCTTGCTGTTACCAAAGAGTGAGTTCTCCTCTTTTCTTCCTCTTTCTGTCGGTTTTCGTGGTGATCAATTTGGTTTCGTGTAACGGGGGATTTGATTTCATCGTTTTTGGTGGGAATTTTGTTTTTCGCGGTTGATGATATATATTGTTCTCTCAGCTGGTTGCGCGGGGGAAGTTTGTCGGAATTTCTATTATAATCGAGCCAGATTTTCTTGATTATTATCTCATCTCGTTGTTTTGTCGAGGGAGGAAATTTTCGGAAATAATTTGTACGACTACGAGTTAGCTATGTAGTATAAACGGATTTGGCTGCTTCTTTTGAATGTTGAAATAGCTTAATTAAATTGCTGCTGTTGAATCGTACCGTAGACTGCTACCACCTTTTCCCCCATTTGTTCGTTTGTCCTCTTTTTTTGACATTTTTTGTAGCCAATTTTAATCTCTAGGTGCATTTGTAACTTGCGTCTGAGAACTTCTTTTTGTTTGCCTACTTTGATTTTCTTACAGGCTTTTGTTTGTTACTAGTTGAGCTTATCTCCTTCCCGAAGTAACTTCATTCCTATGTGTATGTATATTTTTAGTGCAGGTCGGTGAAGACATTCACGTACGAGGCCTTGAATAATGTTGTGAGGCTGATCAACGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAAAGGTTCTGTACTCGAAGGTGATCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAAGTATGTCGATTGACCTGAATTCTATCGCAGTATTTAGGATGTCCAAAATAATCATTCGGAATTGATTTCTCGGCATGATTTTAAATTCACCTTTTCCCGTTAGGGAAGTCCCTGGTTATTATGATGTTCACACTCCCGATATACTTGGAAAATTTGGTTTATTCTTTCAGTGATAAGGATAAGCGGGATAAGATTGCCATGCTTCATTCAATCTACCTCAAAAGTATCAAAACCAATCTTTCAGCACAGTTCTTAATTTGTTTTTCTATCAAGAAAATTTGAGTCACTAATTTTAACGTTTATATTCACCGTATGGTTGAAAGTGGTTTTCTGCCATTCATTCATTCATACTTCGATAAAATCATTCCTAATCTACGTAGGATATAGACTTCTTTTTATTTTTTTATTTTTTGAGAAAATACCTTTAATGCCCGAGAAGATATGTAGGATATAGGATGAATAACTAATAAGGCCAAAGGCATATTGAGGATGGAATTCCTGCAAGGTAATGTAGAGGATTGGAGAGGCGGACATTTTCACTCGGGAAAAGTCAGATGCAATACGAGAAATTAAAATTTGTTTTTCATGTTTGCGTGAAATTATAGTGCGTTAAGGGCAATGTTTGAGATGAAGTTTGGAATAGTATCCTGAATGCATGAATCCACAATTGCACTTGTTTGTTTCAGTAGGGATAAAACGATGTCCTCCATAATTTTTTTTTTTTTAAAGAAGAAGAAAAAAGAAAGAAAGACAGTGTGATATTGCGCTCACCTGTTTGAAACATGACTGTTGGCTCCACCTTCCTGCATGATTATGTGCTGGTACCCTAGTTTTATTGGAAAGAACCATTGTACCCTCATGGGTGGTAATTTTATGTTCTCCATATAACCGTGCAGCTTTCAGAGTTGACGAAGTCACTTGAGCAATTCTGAACTTTAAGTTATATGATAATGTAAAAAAGATAAGTAGGACTCCCCACAATACAAGTGCCTTTTTTTCCTGACGATAAGTAGTTGCTTTTCCAGATGGAATGAGTATTTCTTTTCTTTTACCCTCTGGTAGTTTGAATTGTCATGTCATTGCCATAGAGGACACGTATTACATTTTGTTTTTTTGCTATTTAGGTAAATAAATAGTTGACATGTATATTTTTTTGTTCTGTGATACATAGTGGCGTGTCCTCTTTCAACCAGTTCATTCATGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTTTTGGAAATGAATATCCTGCAACACCATTATCTCAGACTTCTCGAGGCTCCAGGTCCAGGGCTGGTTTTCATGTCAAGAATGTTTGGCACTGGACAGAAAGGATCAGAAGCATCTTTTTTGGATTCTTTTATCCGCAAAGTTCCTCTTGGGGATTCCATTTCGTATTTTTCACTTCTTTTTTATTAGATGGTCAGGGTCCTCATCCACCCCTGGAAGTCCTTGGCCATCAATTAAGAGAGCGCATAGCCATAAGGACCATAATGTTGTTCATCGCACCACTGACAGGAGACGTGGAGTTATTGAGGTTTCTCCCTTACATCTACTACTAGTCTAGTAGTTTCTGGTTCTCCTACCCTTGACTTCACTTAATACATTTAGATATGCTCTACTTTATCCGACTTCTATTTATTAATCTCTCTTTTCTGTACTTGTTAATTAGGATCTTCATCTTGCGAGTGAAATCTTTATTGAATCTGTCTTTGAAATGGTCCATAAGGCTGCACATTTTATCCTCTCGCCATTAGATGCAATTAGAACTTTGTTCAGATGGCTTTTTTCTCGGGGCACTTGGGAGAGAGATCATGATGCAGATGTCATCGTACCTACAGCAACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACTTACACTTTTCATCAGTCACTGAATACAGATGCCAGAACATGCCAAGATGTTATTACGGAGCTTGGGTAAGATTAGTGCTAGTGACTTCTCAATTTAGTTCCAAATTTTGGGTTCAGCATGAAGCTGTTGGTTTCTCATATTTCTTCTGTCTTTCTTCTTATCTTAGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATCCCCAGGTATGAATTCTAGTTAATTTATTCTTGCCTATTTCAATTTTTTGTCCGAATGTTTTGTGGTTAAGGCTTGCACTGTGATTTTCAGGCGGGATGCACGTAAGGCTCTTTATTTGCAGCATGGAATATTTGATTCTTCCATGGGGTGAGGTTTCCTCTCCCATTTCTTGCCAGTTTTTGTTTCACAGTTGTACATAGTTGTACATAGTCTGTTAGTTTAATCTTGTGATGTAGGATAGATAGGGAATGCATTCTGACTTTATGTTGGGTTGGTTAGGGGCCATGACATCCTTAAATTTATTGGCAGGCTAATGAATTGCAAATGTAGTTAAAAATATTCGAACTCTGATAACACAAAGTAACATCTTAAGGATTTCACATGTAACGTAGATTTAATTCTGAAGAACTCCACCGCCCACCACACACACAACCACACGCACGGAGCATAAAGACAAGGTTGCGCTCATTAATTTTTTATTGTCTAGGTTGGATGGGTGGGGAACTATGATGTAGCATGCTTCTGTTATTGATCTACATCTGTTCTCTAACTGTGCTAATTCTTTTGTCTGACTAGTTGGGTGTCCAATGGGGTTGTTGGTTCGCCCGCCTTTGCAGCTTTTGATCAAGGTATGTTACGCTTGCTTCAGAAGTTCTAGCCTTTTGCAATGACACATCAGGCTTTGTTTATCTACCTCTTTTTTCCCCCTCTTTTTTTTGTTTGTAATTTTTTAATAATTTAAAATAATAAATCGTTTATTTATTTTATTATTGCAGGGTATGATGTCTATCTTGGCAATTTCCGTGGTTTGGTCTCTAGAGAACATATTGATAAGAATATATCTTCAAGACAGTAAGTCTCTGCGTTTTTCACTTTTCCATGTATGAATATTCAGTTCATTTAACATACTAATCCTCAATCGAGGGCTAATAATGTGGAAGCTCGATGTCCTCATGCGTTACGGGGATGTCATTTTACCGACTGATTTTGTGAACTATCCAGGTACTGGAAATATTCCATAAACGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAATGATCAACCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGGGCTGGTATGTTGATGTATGTTATAACACGGAGGATTGAAGAAAAGCCACATAGATTGTCGAGATTGGTCTTACTATCACCTGCTGGTTTCCATGATGATGCTCCGTTTATTTTCACCGTGATTGAGAATCTCTTGCTCTTGTTAGCTCCAATTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACAAGGTTTTTCCGTATGCTTCTAAACAAGTTGGCTCGTGACTTTCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACCATCGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTACTGGGAACTCCGCACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCTAAGAAGTTTAGAATGTTCGATTATGGGAATGCATCCCTGAACATGGAAGCCTATGGATCACCAGAGCCATTGGACTTGGGCGAATACTTCGGGCTTTTCGATATCCCTGTAGATTTAGTTGCTGGACGGAAGGACCAGGTAATAAGACCAACAATGGTGAAAAGGTACTACAAAATGATGAAAGAGGCTGGTGTTGAGGTATCATTCAATGAATTTGAGTACGCCCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTGATGTCTCGTCTACTTCTGGTCAATGAACCCGGATCAAATTGA

mRNA sequence

ATGATTCAACGCTTTGTCGACGATGTTCTTGCTGTTACCAAAGAGTCGGTGAAGACATTCACGTACGAGGCCTTGAATAATGTTGTGAGGCTGATCAACGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAAAGGTTCTGTACTCGAAGGTGATCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAATGGCGTGTCCTCTTTCAACCAGTTCATTCATGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTTTTGGAAATGAATATCCTGCAACACCATTATCTCAGACTTCTCGAGGCTCCAGATGGTCAGGGTCCTCATCCACCCCTGGAAGTCCTTGGCCATCAATTAAGAGAGCGCATAGCCATAAGGACCATAATGTTGTTCATCGCACCACTGACAGGAGACGTGGAGTTATTGAGGATCTTCATCTTGCGAGTGAAATCTTTATTGAATCTGTCTTTGAAATGGTCCATAAGGCTGCACATTTTATCCTCTCGCCATTAGATGCAATTAGAACTTTGTTCAGATGGCTTTTTTCTCGGGGCACTTGGGAGAGAGATCATGATGCAGATGTCATCGTACCTACAGCAACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACTTACACTTTTCATCAGTCACTGAATACAGATGCCAGAACATGCCAAGATGTTATTACGGAGCTTGGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATCCCCAGGCGGGATGCACGTAAGGCTCTTTATTTGCAGCATGGAATATTTGATTCTTCCATGGGTTGGGTGTCCAATGGGGTTGTTGGTTCGCCCGCCTTTGCAGCTTTTGATCAAGGGTATGATGTCTATCTTGGCAATTTCCGTGGTTTGGTCTCTAGAGAACATATTGATAAGAATATATCTTCAAGACAGTACTGGAAATATTCCATAAACGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAATGATCAACCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGGGCTGGTATGTTGATGTATGTTATAACACGGAGGATTGAAGAAAAGCCACATAGATTGTCGAGATTGGTCTTACTATCACCTGCTGGTTTCCATGATGATGCTCCGTTTATTTTCACCGTGATTGAGAATCTCTTGCTCTTGTTAGCTCCAATTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACAAGGTTTTTCCGTATGCTTCTAAACAAGTTGGCTCGTGACTTTCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACCATCGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTACTGGGAACTCCGCACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCTAAGAAGTTTAGAATGTTCGATTATGGGAATGCATCCCTGAACATGGAAGCCTATGGATCACCAGAGCCATTGGACTTGGGCGAATACTTCGGGCTTTTCGATATCCCTGTAGATTTAGTTGCTGGACGGAAGGACCAGGTAATAAGACCAACAATGGTGAAAAGGTACTACAAAATGATGAAAGAGGCTGGTGTTGAGGTATCATTCAATGAATTTGAGTACGCCCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTGATGTCTCGTCTACTTCTGGTCAATGAACCCGGATCAAATTGA

Coding sequence (CDS)

ATGATTCAACGCTTTGTCGACGATGTTCTTGCTGTTACCAAAGAGTCGGTGAAGACATTCACGTACGAGGCCTTGAATAATGTTGTGAGGCTGATCAACGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAAAGGTTCTGTACTCGAAGGTGATCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAATGGCGTGTCCTCTTTCAACCAGTTCATTCATGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTTTTGGAAATGAATATCCTGCAACACCATTATCTCAGACTTCTCGAGGCTCCAGATGGTCAGGGTCCTCATCCACCCCTGGAAGTCCTTGGCCATCAATTAAGAGAGCGCATAGCCATAAGGACCATAATGTTGTTCATCGCACCACTGACAGGAGACGTGGAGTTATTGAGGATCTTCATCTTGCGAGTGAAATCTTTATTGAATCTGTCTTTGAAATGGTCCATAAGGCTGCACATTTTATCCTCTCGCCATTAGATGCAATTAGAACTTTGTTCAGATGGCTTTTTTCTCGGGGCACTTGGGAGAGAGATCATGATGCAGATGTCATCGTACCTACAGCAACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACTTACACTTTTCATCAGTCACTGAATACAGATGCCAGAACATGCCAAGATGTTATTACGGAGCTTGGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATCCCCAGGCGGGATGCACGTAAGGCTCTTTATTTGCAGCATGGAATATTTGATTCTTCCATGGGTTGGGTGTCCAATGGGGTTGTTGGTTCGCCCGCCTTTGCAGCTTTTGATCAAGGGTATGATGTCTATCTTGGCAATTTCCGTGGTTTGGTCTCTAGAGAACATATTGATAAGAATATATCTTCAAGACAGTACTGGAAATATTCCATAAACGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAATGATCAACCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGGGCTGGTATGTTGATGTATGTTATAACACGGAGGATTGAAGAAAAGCCACATAGATTGTCGAGATTGGTCTTACTATCACCTGCTGGTTTCCATGATGATGCTCCGTTTATTTTCACCGTGATTGAGAATCTCTTGCTCTTGTTAGCTCCAATTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACAAGGTTTTTCCGTATGCTTCTAAACAAGTTGGCTCGTGACTTTCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACCATCGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTACTGGGAACTCCGCACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCTAAGAAGTTTAGAATGTTCGATTATGGGAATGCATCCCTGAACATGGAAGCCTATGGATCACCAGAGCCATTGGACTTGGGCGAATACTTCGGGCTTTTCGATATCCCTGTAGATTTAGTTGCTGGACGGAAGGACCAGGTAATAAGACCAACAATGGTGAAAAGGTACTACAAAATGATGAAAGAGGCTGGTGTTGAGGTATCATTCAATGAATTTGAGTACGCCCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTGATGTCTCGTCTACTTCTGGTCAATGAACCCGGATCAAATTGA

Protein sequence

MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRPTFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGSRWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELLAYVMSRLLLVNEPGSN
Homology
BLAST of Lag0024264 vs. NCBI nr
Match: XP_023000889.1 (lipase member N isoform X2 [Cucurbita maxima])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 596/630 (94.60%), Postives = 610/630 (96.83%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKA 180
           R     S+PGSPWPSIKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKA
Sbjct: 121 R-----SSPGSPWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKA 180

Query: 181 AHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240
           AHFILSPLD IRTLFRWL+S  + ERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD
Sbjct: 181 AHFILSPLDTIRTLFRWLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240

Query: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGV 300
           ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGV
Sbjct: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGV 300

Query: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360
           VGSPAFAAFDQGYDVYLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHE
Sbjct: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360

Query: 361 VKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLS 420
           VKTSELKLSQPELAEETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLS
Sbjct: 361 VKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLS 420

Query: 421 PAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480
           PAGFHDDAPFIFTVIENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL
Sbjct: 421 PAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480

Query: 481 VQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540
           VQT+VSYFLGGDSSNWVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL
Sbjct: 481 VQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540

Query: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFE 600
           NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFE
Sbjct: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFE 600

Query: 601 YAHLDFTFSHREELLAYVMSRLLLVNEPGS 631
           YAHLDFTFSHREELLAYVMSRLLLV EPGS
Sbjct: 601 YAHLDFTFSHREELLAYVMSRLLLVGEPGS 624

BLAST of Lag0024264 vs. NCBI nr
Match: XP_022923984.1 (lipase member N isoform X2 [Cucurbita moschata])

HSP 1 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 594/630 (94.29%), Postives = 608/630 (96.51%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKA 180
           R     S+PGSPWPSIKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKA
Sbjct: 121 R-----SSPGSPWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKA 180

Query: 181 AHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240
           AHFILSPLD IRTLFRWL+S  + ERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTD
Sbjct: 181 AHFILSPLDTIRTLFRWLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTD 240

Query: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGV 300
           ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGV
Sbjct: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGV 300

Query: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360
           VGSPAFAAFDQGYDVYLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHE
Sbjct: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360

Query: 361 VKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLS 420
           VKTSELKLSQPELAEETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLS
Sbjct: 361 VKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLS 420

Query: 421 PAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480
           PAGFHDDAPFIFTVIENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL
Sbjct: 421 PAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480

Query: 481 VQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540
           VQT+VSYFLGGDSSNWVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNAS 
Sbjct: 481 VQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASQ 540

Query: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFE 600
           NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFE
Sbjct: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFE 600

Query: 601 YAHLDFTFSHREELLAYVMSRLLLVNEPGS 631
           YAHLDFTFSHREELLAYVMSRLLLV EPGS
Sbjct: 601 YAHLDFTFSHREELLAYVMSRLLLVGEPGS 624

BLAST of Lag0024264 vs. NCBI nr
Match: XP_023000888.1 (uncharacterized protein LOC111495192 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 601/675 (89.04%), Postives = 615/675 (91.11%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 R---------------------------------------------WSGSSSTPGSPWPS 180
           R                                             WSGSSS+PGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLF 240
           IKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKAAHFILSPLD IRTLF
Sbjct: 181 IKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLF 240

Query: 241 RWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300
           RWL+S  + ERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY
Sbjct: 241 RWLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300

Query: 301 EAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360
           EAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV
Sbjct: 301 EAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360

Query: 361 YLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420
           YLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE
Sbjct: 361 YLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420

Query: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480
           ETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI
Sbjct: 421 ETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480

Query: 481 ENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSN 540
           ENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQT+VSYFLGGDSSN
Sbjct: 481 ENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSN 540

Query: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600
           WVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE
Sbjct: 541 WVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600

Query: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELL 631
           YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFEYAHLDFTFSHREELL
Sbjct: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELL 660

BLAST of Lag0024264 vs. NCBI nr
Match: XP_023519486.1 (lipase member N isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1201.0 bits (3106), Expect = 0.0e+00
Identity = 593/630 (94.13%), Postives = 609/630 (96.67%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKA 180
           R     S+PGSPWPSIKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKA
Sbjct: 121 R-----SSPGSPWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKA 180

Query: 181 AHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240
           AHFILSPL+ IRTLFRWL+S  + ERDH+ADVIVPTATLGDDDPVPSERTYTFHQSLNTD
Sbjct: 181 AHFILSPLETIRTLFRWLWSWSSCERDHNADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240

Query: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGV 300
           ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGV
Sbjct: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGV 300

Query: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360
           VGSPAFAAFDQGYDVYLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHE
Sbjct: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360

Query: 361 VKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLS 420
           VKTSELKLSQPELAEETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLS
Sbjct: 361 VKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLS 420

Query: 421 PAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480
           PAGFHDDAPFIFTVIENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL
Sbjct: 421 PAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480

Query: 481 VQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540
           VQT+VSYFLGGDSSNWVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL
Sbjct: 481 VQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540

Query: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFE 600
           NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFE
Sbjct: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFE 600

Query: 601 YAHLDFTFSHREELLAYVMSRLLLVNEPGS 631
           YAHLDFTFSHREELLAYVMSRLLLV E GS
Sbjct: 601 YAHLDFTFSHREELLAYVMSRLLLVGESGS 624

BLAST of Lag0024264 vs. NCBI nr
Match: KAG6584371.1 (Lipase member N, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019955.1 Lipase member N [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 600/675 (88.89%), Postives = 614/675 (90.96%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 R---------------------------------------------WSGSSSTPGSPWPS 180
           R                                             WSGSSS+PGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLF 240
           IKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKAAHFILSPLD IRTLF
Sbjct: 181 IKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLF 240

Query: 241 RWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300
           RWL+S  + ERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY
Sbjct: 241 RWLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300

Query: 301 EAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360
           EAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV
Sbjct: 301 EAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360

Query: 361 YLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420
           YLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE
Sbjct: 361 YLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420

Query: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480
           ETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI
Sbjct: 421 ETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480

Query: 481 ENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSN 540
           ENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQT+VSYFLGGDSSN
Sbjct: 481 ENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSN 540

Query: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600
           WVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE
Sbjct: 541 WVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600

Query: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELL 631
           YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFEYAHLDFTFSHREELL
Sbjct: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELL 660

BLAST of Lag0024264 vs. ExPASy Swiss-Prot
Match: Q8K2A6 (Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.2e-22
Identity = 100/371 (26.95%), Postives = 164/371 (44.20%), Query Frame = 0

Query: 246 DVITELGYPYEAIRVVTNDGYVLLLERIPR-------RDARKALYLQHGIFDSSMGWVSN 305
           ++I   GYP E   V T DGY+L + RIPR         +R  + LQHG+   +  W+SN
Sbjct: 51  EIIKHKGYPSEEYEVATEDGYILSVNRIPRGQTRLKKEGSRPVVLLQHGLLGDASNWISN 110

Query: 306 GVVGSPAFAAFDQGYDVYLGNFRGLV-SREHIDKNISSRQYWKYSINEHAMEDIPAMIEK 365
               S  F   D G+DV++GN RG   SR+H   +I   ++W +S +E A  D+PA+I  
Sbjct: 111 LPNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINF 170

Query: 366 IHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLV 425
           I + KT + K+     ++ T             +MG      ++    + E  H++    
Sbjct: 171 ILQ-KTGQKKVYYVGYSQGT-------------TMG------FIAFSTMPELAHKIKMYF 230

Query: 426 LLSPAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAV 485
            L+P      A    T    L  ++  +L      LY  TRFFR L   L         +
Sbjct: 231 ALAPIATVKYARSPGTKFLLLPDMMIKVLFGRQEFLY-QTRFFRQLFIYLCGQM-----I 290

Query: 486 GGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGN 545
              + + +   LGG ++N + +     Y  +   G S +  LH +Q  ++ + R FD+G+
Sbjct: 291 LDQICSNIILLLGGFNTNNMNMSRANVYVAHTPAGTSVQNILHWSQAVNSGELRAFDWGS 350

Query: 546 ASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVS 605
            + N E    P P+     + + D  +P  +  G +D +  P  VK    + +   +   
Sbjct: 351 ETKNQEKCNQPTPI----RYKVRDMMVPTAMWTGGQDWLSNPDDVKTL--LSEVTNLIYH 389

Query: 606 FNEFEYAHLDF 607
            N  E+AH+DF
Sbjct: 411 KNIPEWAHVDF 389

BLAST of Lag0024264 vs. ExPASy Swiss-Prot
Match: Q5VYY2 (Lipase member M OS=Homo sapiens OX=9606 GN=LIPM PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 4.4e-22
Identity = 99/379 (26.12%), Postives = 167/379 (44.06%), Query Frame = 0

Query: 246 DVITELGYPYEAIRVVTNDGYVLLLERIPR-------RDARKALYLQHGIFDSSMGWVSN 305
           ++I   GYP E   V T DGY+L + RIPR         +R  + LQHG+   +  W+SN
Sbjct: 51  EIIQHQGYPCEEYEVATEDGYILSVNRIPRGLVQPKKTGSRPVVLLQHGLVGGASNWISN 110

Query: 306 GVVGSPAFAAFDQGYDVYLGNFRGLV-SREHIDKNISSRQYWKYSINEHAMEDIPAMIEK 365
               S  F   D G+DV++GN RG   SR+H   +I   ++W +S +E A  D+PA+I  
Sbjct: 111 LPNNSLGFILADAGFDVWMGNSRGNAWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINF 170

Query: 366 IHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLV 425
           I + KT + K+     ++ T             +MG      ++    + E   ++    
Sbjct: 171 ILQ-KTGQEKIYYVGYSQGT-------------TMG------FIAFSTMPELAQKIKMYF 230

Query: 426 LLSPAGF--HDDAPFIFTVIENLLLLLAPILAPFVPGLY------IPTRFFRMLLNKLAR 485
            L+P     H  +P           LL P +   + GL+        TRF R L+  L  
Sbjct: 231 ALAPIATVKHAKSP-------GTKFLLLPDM--MIKGLFGKKEFLYQTRFLRQLVIYLCG 290

Query: 486 DFHHYPAVGGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKK 545
                  +   + + +   LGG ++N + +     Y  + + G S +  LH +Q  ++ +
Sbjct: 291 Q-----VILDQICSNIMLLLGGFNTNNMNMSRASVYAAHTLAGTSVQNILHWSQAVNSGE 350

Query: 546 FRMFDYGNASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRPTMVKRYYKMM 605
            R FD+G+ + N+E    P P+     + + D  +P  +  G +D +  P  VK    + 
Sbjct: 351 LRAFDWGSETKNLEKCNQPTPV----RYRVRDMTVPTAMWTGGQDWLSNPEDVKML--LS 389

Query: 606 KEAGVEVSFNEFEYAHLDF 607
           +   +    N  E+AH+DF
Sbjct: 411 EVTNLIYHKNIPEWAHVDF 389

BLAST of Lag0024264 vs. ExPASy Swiss-Prot
Match: P80035 (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 1.7e-21
Identity = 99/344 (28.78%), Postives = 157/344 (45.64%), Query Frame = 0

Query: 247 VITELGYPYEAIRVVTNDGYVLLLERIP--RRDA-----RKALYLQHGIFDSSMGWVSNG 306
           +IT  GYP E   VVT DGY+L ++RIP  R+++     R   +LQHG+  S+  W+SN 
Sbjct: 38  MITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNL 97

Query: 307 VVGSPAFAAFDQGYDVYLGNFRGLV-SREHIDKNISSRQYWKYSINEHAMEDIPAMIEKI 366
              S AF   D GYDV+LGN RG   +R ++  +  S ++W +S +E A  D+PA I+ I
Sbjct: 98  PNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFI 157

Query: 367 HEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGA-GMLMYVITRRIEEKPHRLSRLV 426
                           ++T  D   KL  + HS G   G + +    ++ +   R+    
Sbjct: 158 ---------------LKKTGQD---KLHYVGHSQGTTIGFIAFSTNPKLAK---RIKTFY 217

Query: 427 LLSPAGFHDDAPFIFTVIENLLLLLAPILAPFVPG--LYIPTRFFRMLLNKLARDFHHYP 486
            L+P        +  T++ N L+L+   L   + G  ++ P  FF      LA +     
Sbjct: 218 ALAPVA---TVKYTETLL-NKLMLVPSFLFKLIFGNKIFYPHHFFDQF---LATEVCSRE 277

Query: 487 AVGGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDY 546
            V  L    +    G D+ N + +     Y  ++  G S +  LH +Q   + KF+ FD+
Sbjct: 278 TVDLLCSNALFIICGFDTMN-LNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDW 337

Query: 547 GNASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRP 578
           G+   NM  Y    P     Y+ L D  +P+ +  G  D +  P
Sbjct: 338 GSPVQNMMHYHQSMP----PYYNLTDMHVPIAVWNGGNDLLADP 348

BLAST of Lag0024264 vs. ExPASy Swiss-Prot
Match: Q3U4B4 (Lipase member N OS=Mus musculus OX=10090 GN=Lipn PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.7e-21
Identity = 105/392 (26.79%), Postives = 163/392 (41.58%), Query Frame = 0

Query: 233 FHQSLNTDA-RTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDA-------RKALYL 292
           F   +N +      ++I   GYP E   V T DGY+L + RIP   A       R  +Y+
Sbjct: 26  FENKVNPEVWMNASEIIMYNGYPSEEYDVTTADGYILAINRIPHGRAQTGQTGPRPVVYM 85

Query: 293 QHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVYLGNFRGLV-SREHIDKNISSRQYWKYSI 352
           QH +F  +  W+ N   GS  F   D GYDV++GN RG   SR H   + +  ++W +S 
Sbjct: 86  QHALFADNAYWLENFANGSLGFILADAGYDVWMGNSRGNTWSRRHKTLSANEEKFWAFSF 145

Query: 353 NEHAMEDIPAMIEKIHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVIT 412
           NE A  D+P +I+ I      E                  KL  + HS+G    + +V  
Sbjct: 146 NEMAKYDLPGIIDFIVNKTGQE------------------KLYFIGHSLGTT--IGFVAF 205

Query: 413 RRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRML 472
             + E   R+     L P         +FT   NL LL   I+      L   T+   +L
Sbjct: 206 STMPELAQRIKMNFALGPVISFKYPTSVFT---NLFLLPKSIIK-----LVFGTKGV-LL 265

Query: 473 LNKLAR----DFHHYPAVGGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGL 532
            +K AR     F +   +  L    +S + G +  N + +     Y  +   G S +  L
Sbjct: 266 EDKNARMSFITFCNQKLLQPLCSEFMSLWAGFNKKN-MNMSRLDVYMAHAPTGSSIQNML 325

Query: 533 HLAQMKHAKKFRMFDYGNASLNMEAYGSPEP--LDLGEYFGLFDIPVDLVAGRKDQVIRP 592
           H+ Q+  + +FR +D+G+ + NM  Y    P   DL        +P  + AG  D ++ P
Sbjct: 326 HIKQLYRSDEFRAYDWGSEAENMNHYNQSYPPLYDL----TAMKVPTAIWAGGHDVLVTP 380

Query: 593 TMVKRYYKMMKEAGVEVSFNEF-EYAHLDFTF 609
             V R    +        F +F ++ H DF +
Sbjct: 386 QDVARILPQITNLRY---FKQFPDWNHFDFVW 380

BLAST of Lag0024264 vs. ExPASy Swiss-Prot
Match: O46108 (Lipase 3 OS=Drosophila melanogaster OX=7227 GN=Lip3 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.2e-19
Identity = 106/398 (26.63%), Postives = 154/398 (38.69%), Query Frame = 0

Query: 244 CQDVITELGYPYEAIRVVTNDGYVLLLERIP------RRDARKALYLQHGIFDSSMGWVS 303
           C + I + GYP E   VVT+D Y+L + RIP          R   +L HG+  SS  WV 
Sbjct: 28  CGERIEDDGYPMERHEVVTSDNYILTMHRIPYSPKTGESSNRPVAFLMHGMLSSSSDWVL 87

Query: 304 NGVVGSPAFAAFDQGYDVYLGNFRG-LVSREHIDKNISSRQYWKYSINEHAMEDIPAMIE 363
            G   S A+   D GYDV++GN RG   S+ H       + +W +S NE  M D+PAMI+
Sbjct: 88  MGPERSLAYMLADAGYDVWMGNARGNTYSKAHKYWPTYWQIFWNFSWNEIGMYDVPAMID 147

Query: 364 KIHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRL 423
            +   KT + ++                   + HS G    L+ V  R   E   ++   
Sbjct: 148 YV-LAKTGQQQVQY-----------------VGHSQGTTVYLVMVSER--PEYNDKIKSA 207

Query: 424 VLLSPAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFR-------MLLNKLAR 483
            LL PA +  +             + +P+   F P L  P            M  NK  +
Sbjct: 208 HLLGPAAYMGN-------------MKSPLTRAFAPILGQPNAIVEVCGSMEFMPSNKFKQ 267

Query: 484 D--FHHYPAVGGLVQTIVS--YFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMK 543
           D       A         +  + +GG  +  +      H       G S    LH  Q  
Sbjct: 268 DLGIEMCQATSPYADMCANEIFLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEY 327

Query: 544 HAKKFRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYK 603
           ++ KFR FDY  A  N   YGS  P D          PV L  G  D +   + V++   
Sbjct: 328 NSGKFRKFDY-TALRNPYEYGSYFPPDY--KLKNAKAPVLLYYGANDWMCDVSDVRKLRD 387

Query: 604 MMKEAGVEVSFNEFEYAHLDFTFSHREELLAYVMSRLL 624
            +    ++      ++AHLDF +    E   YV   +L
Sbjct: 388 ELPNMALDYLVPFEKWAHLDFIWG--TEARKYVYDEVL 387

BLAST of Lag0024264 vs. ExPASy TrEMBL
Match: A0A6J1KL85 (lipase member N isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 596/630 (94.60%), Postives = 610/630 (96.83%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKA 180
           R     S+PGSPWPSIKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKA
Sbjct: 121 R-----SSPGSPWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKA 180

Query: 181 AHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240
           AHFILSPLD IRTLFRWL+S  + ERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD
Sbjct: 181 AHFILSPLDTIRTLFRWLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240

Query: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGV 300
           ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGV
Sbjct: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGV 300

Query: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360
           VGSPAFAAFDQGYDVYLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHE
Sbjct: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360

Query: 361 VKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLS 420
           VKTSELKLSQPELAEETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLS
Sbjct: 361 VKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLS 420

Query: 421 PAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480
           PAGFHDDAPFIFTVIENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL
Sbjct: 421 PAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480

Query: 481 VQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540
           VQT+VSYFLGGDSSNWVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL
Sbjct: 481 VQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540

Query: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFE 600
           NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFE
Sbjct: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFE 600

Query: 601 YAHLDFTFSHREELLAYVMSRLLLVNEPGS 631
           YAHLDFTFSHREELLAYVMSRLLLV EPGS
Sbjct: 601 YAHLDFTFSHREELLAYVMSRLLLVGEPGS 624

BLAST of Lag0024264 vs. ExPASy TrEMBL
Match: A0A6J1E7W0 (lipase member N isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431539 PE=4 SV=1)

HSP 1 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 594/630 (94.29%), Postives = 608/630 (96.51%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RWSGSSSTPGSPWPSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKA 180
           R     S+PGSPWPSIKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKA
Sbjct: 121 R-----SSPGSPWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKA 180

Query: 181 AHFILSPLDAIRTLFRWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTD 240
           AHFILSPLD IRTLFRWL+S  + ERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTD
Sbjct: 181 AHFILSPLDTIRTLFRWLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTD 240

Query: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGV 300
           ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGV
Sbjct: 241 ARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGV 300

Query: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360
           VGSPAFAAFDQGYDVYLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHE
Sbjct: 301 VGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHE 360

Query: 361 VKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLS 420
           VKTSELKLSQPELAEETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLS
Sbjct: 361 VKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLS 420

Query: 421 PAGFHDDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480
           PAGFHDDAPFIFTVIENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL
Sbjct: 421 PAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGL 480

Query: 481 VQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASL 540
           VQT+VSYFLGGDSSNWVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNAS 
Sbjct: 481 VQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASQ 540

Query: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFE 600
           NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFE
Sbjct: 541 NMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFE 600

Query: 601 YAHLDFTFSHREELLAYVMSRLLLVNEPGS 631
           YAHLDFTFSHREELLAYVMSRLLLV EPGS
Sbjct: 601 YAHLDFTFSHREELLAYVMSRLLLVGEPGS 624

BLAST of Lag0024264 vs. ExPASy TrEMBL
Match: A0A6J1KNX8 (uncharacterized protein LOC111495192 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 601/675 (89.04%), Postives = 615/675 (91.11%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 R---------------------------------------------WSGSSSTPGSPWPS 180
           R                                             WSGSSS+PGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLF 240
           IKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKAAHFILSPLD IRTLF
Sbjct: 181 IKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLF 240

Query: 241 RWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300
           RWL+S  + ERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY
Sbjct: 241 RWLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300

Query: 301 EAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360
           EAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV
Sbjct: 301 EAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360

Query: 361 YLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420
           YLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE
Sbjct: 361 YLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420

Query: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480
           ETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI
Sbjct: 421 ETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480

Query: 481 ENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSN 540
           ENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQT+VSYFLGGDSSN
Sbjct: 481 ENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSN 540

Query: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600
           WVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE
Sbjct: 541 WVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600

Query: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELL 631
           YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFEYAHLDFTFSHREELL
Sbjct: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELL 660

BLAST of Lag0024264 vs. ExPASy TrEMBL
Match: A0A6J1EB22 (uncharacterized protein LOC111431539 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431539 PE=4 SV=1)

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 599/675 (88.74%), Postives = 613/675 (90.81%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPG+GSVLEG HGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATP SQ+SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 R---------------------------------------------WSGSSSTPGSPWPS 180
           R                                             WSGSSS+PGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLF 240
           IKR HSHK+H VVH TTDRRRGVIEDLHLASEIFIES FEMVHKAAHFILSPLD IRTLF
Sbjct: 181 IKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLF 240

Query: 241 RWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300
           RWL+S  + ERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY
Sbjct: 241 RWLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300

Query: 301 EAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360
           EAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV
Sbjct: 301 EAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360

Query: 361 YLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420
           YLGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE
Sbjct: 361 YLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420

Query: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480
           ETD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI
Sbjct: 421 ETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480

Query: 481 ENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSN 540
           ENLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQT+VSYFLGGDSSN
Sbjct: 481 ENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSN 540

Query: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600
           WVGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNAS NMEAYGSPEPLDLGE
Sbjct: 541 WVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASQNMEAYGSPEPLDLGE 600

Query: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELL 631
           YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMK+AGV+VSFNEFEYAHLDFTFSHREELL
Sbjct: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELL 660

BLAST of Lag0024264 vs. ExPASy TrEMBL
Match: A0A1S3C3R6 (uncharacterized protein LOC103496491 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496491 PE=4 SV=1)

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 589/675 (87.26%), Postives = 607/675 (89.93%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRP 60
           MIQR VDDVLAVTKESVKTFTYE+LNNVVRLINGLSALLLTLLPGKGSVLEG +GWELRP
Sbjct: 1   MIQRVVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGKGSVLEGVNGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATP SQ SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPSSQGSRGS 120

Query: 121 R---------------------------------------------WSGSSSTPGSPWPS 180
           R                                             WSGS++TPGSPWPS
Sbjct: 121 RSRAGFHAKNVWHWTERIRSIIFWILLPARFLFGIPFRIFHFFFIKWSGSTTTPGSPWPS 180

Query: 181 IKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLF 240
           I+R HSHKDH VVH TTDRRRGVIEDLHLASEIFIESVFE+VHKAAHFILSPLDA+RT F
Sbjct: 181 IRRIHSHKDH-VVH-TTDRRRGVIEDLHLASEIFIESVFEIVHKAAHFILSPLDALRTSF 240

Query: 241 RWLFSRGTWERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPY 300
           RWLF R + E  HDADV VPTATLGDDD  PSER+YTFHQSLNTDARTCQDVITELGYPY
Sbjct: 241 RWLFPRDSCESYHDADVTVPTATLGDDDSAPSERSYTFHQSLNTDARTCQDVITELGYPY 300

Query: 301 EAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360
           EAIRVVTNDGYVLLLERIPRRDARK LYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV
Sbjct: 301 EAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV 360

Query: 361 YLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAE 420
           +LGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKI+EVKTSELKLSQPE+ +
Sbjct: 361 FLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIYEVKTSELKLSQPEIVK 420

Query: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480
           ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI
Sbjct: 421 ETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVI 480

Query: 481 ENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSSN 540
           ENLLLLLAPILAPFVPGLYIPTRF RMLLNKLARDF HYPAVGGLVQT+VSYFLGGDSSN
Sbjct: 481 ENLLLLLAPILAPFVPGLYIPTRFCRMLLNKLARDFQHYPAVGGLVQTVVSYFLGGDSSN 540

Query: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGE 600
           WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGN SLNMEAYGSPEPLDLGE
Sbjct: 541 WVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNPSLNMEAYGSPEPLDLGE 600

Query: 601 YFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREELL 631
           YFGL DIPVDLVAGRKDQVIRPTMVKRYY MMK+AGV+VSFNEFEYAHLDFTFSHREELL
Sbjct: 601 YFGLIDIPVDLVAGRKDQVIRPTMVKRYYSMMKDAGVDVSFNEFEYAHLDFTFSHREELL 660

BLAST of Lag0024264 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 882.1 bits (2278), Expect = 2.7e-256
Identity = 438/682 (64.22%), Postives = 526/682 (77.13%), Query Frame = 0

Query: 2   IQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRPT 61
           +QR VD+ LAVTKESVKT TYE+LNN+ R ING+SALLLTLLPGK ++LEG HGWELRPT
Sbjct: 1   MQRIVDNALAVTKESVKTVTYESLNNIARCINGVSALLLTLLPGKANILEGLHGWELRPT 60

Query: 62  FRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGSR 121
           FRGPR PRWM NGVSSFN+FIHELSVDSD SSL+YSSGE+       P +P SQ+SR S 
Sbjct: 61  FRGPRLPRWMHNGVSSFNEFIHELSVDSDTSSLEYSSGEDS--DGPLPPSPSSQSSRLS- 120

Query: 122 WSGSSSTPGSPW------------------------------------------------ 181
           W+ +S++  + W                                                
Sbjct: 121 WASTSASSENHWTEWITFILWWLMFPVRILLWIPQYIISLFYKRSSRIPASPRRHQHSSR 180

Query: 182 PSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRT 241
           P I + +S KDH+V +RTTDRRRGVIEDLHLA EI IE++F+  HKA H +LSP +A   
Sbjct: 181 PRISKTNSSKDHDVPNRTTDRRRGVIEDLHLAIEIGIEAIFDFFHKATHLLLSPSEAFAI 240

Query: 242 LFRWLFSRGTWERDHDADV----IVPTATLGDDDPVPSERTY----TFHQSLNTDARTCQ 301
           L  W  S     +++  +V     V TATLGD DP P+ER        + S+NTD RTCQ
Sbjct: 241 LLSWFSSSSRSPKENHGEVAYDETVQTATLGDTDPSPTERPVESPTRLYNSMNTDTRTCQ 300

Query: 302 DVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPA 361
           DVITELGYPYEAIRV+T+DGYVL+LERIPRRDARKA++LQHG+ DSSMGWVSNGVVGSPA
Sbjct: 301 DVITELGYPYEAIRVITSDGYVLVLERIPRRDARKAVFLQHGVLDSSMGWVSNGVVGSPA 360

Query: 362 FAAFDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSE 421
           FAA+DQGYDV+LGNFRGLVSR+H++KNISS+++W+YSINEH  EDIPAMIEKIHE+KT+E
Sbjct: 361 FAAYDQGYDVFLGNFRGLVSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTTE 420

Query: 422 LKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFH 481
           LKL QP + EE + ++P+KLCA+CHS+GGA +LMYVITR+I+EKPHRLSRL+LLSPAGFH
Sbjct: 421 LKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLILLSPAGFH 480

Query: 482 DDAPFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIV 541
           +D+   FT++E + L ++P+LA  VP  YIPTRFFRMLLNKLARDFH+YPA+GGLVQT++
Sbjct: 481 EDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALGGLVQTLM 540

Query: 542 SYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAY 601
           SY +GGDSSNWVGVLG PHYNMNDMP VSFRV  HLAQ+KH  KFRM+DYG+ S NME Y
Sbjct: 541 SYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEVY 600

Query: 602 GSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLD 628
           GSPEPLDLGE +   D+PVDLVAGR D+VIR +MVK++Y +M++A V+VSFNEFEYAHLD
Sbjct: 601 GSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHLD 660

BLAST of Lag0024264 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 874.0 bits (2257), Expect = 7.2e-254
Identity = 437/677 (64.55%), Postives = 518/677 (76.51%), Query Frame = 0

Query: 2   IQRFVDDVLAVTKESVKTFTYEALNNVVRLINGLSALLLTLLPGKGSVLEGDHGWELRPT 61
           +QR VD+ LA+TKESVKT TYE+LNN+ R ING SALLLTLLPGK +VLEG HGWELRPT
Sbjct: 1   MQRIVDNALAITKESVKTLTYESLNNIARCINGFSALLLTLLPGKSNVLEGLHGWELRPT 60

Query: 62  FRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGSR 121
            RGPR PRWM NGVSSFN FIHELSVDSD SSLDYSSG++D  G   P +PLSQ+S  S 
Sbjct: 61  LRGPRLPRWMHNGVSSFNHFIHELSVDSDTSSLDYSSGDDDSDGMSTPPSPLSQSSLRS- 120

Query: 122 WSGSSSTPGSPW------------------------------------------------ 181
           W+   +   S W                                                
Sbjct: 121 WASLPANYESHWTDWITFIVWWALLPARILLWVPLYLLRLLARRNSRMQPLSPGRYQHSS 180

Query: 182 -PSIKRAHSHKDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIR 241
            P   +A S K+H+V +RTTD+RRGVIEDL L  EIFIE++F+  HKAAH +LSP +   
Sbjct: 181 RPCFSKAISGKEHDVPNRTTDKRRGVIEDLQLGIEIFIETIFDFFHKAAHLLLSPSETFG 240

Query: 242 TLFRWLFSRGTWERDHDADV----IVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVI 301
            +  W  S     + +  DV    I+ TA LGD+D   +ER  T     NTD RTCQDVI
Sbjct: 241 IVLSWFSSSSHSSKGNYGDVSDDEIIQTAILGDNDSSLTERR-TTTSLYNTDTRTCQDVI 300

Query: 302 TELGYPYEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAA 361
           TELGYPYEAIRVVT+DGY LLLERIPRRDARKA+YLQHG+ DSSMGWVSNGVVGSPAFAA
Sbjct: 301 TELGYPYEAIRVVTSDGYGLLLERIPRRDARKAVYLQHGVMDSSMGWVSNGVVGSPAFAA 360

Query: 362 FDQGYDVYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKL 421
           +DQGYDV+LGNFRGLVSR+H+ KNISS+ +W+YSINEHA EDIPAMIEKIHE+KTSELKL
Sbjct: 361 YDQGYDVFLGNFRGLVSRDHVKKNISSKDFWRYSINEHATEDIPAMIEKIHEIKTSELKL 420

Query: 422 SQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDA 481
            QP + E  + DQP+KLC + HS+GGA +LMYVITR+IEEKPHRLSRL+LLSPAGFH D+
Sbjct: 421 YQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKPHRLSRLILLSPAGFHYDS 480

Query: 482 PFIFTVIENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYF 541
              FT++E   L L P+L+  VP  YIPT+FFRMLLNKLARDFH+YPAVGGLVQT++SY 
Sbjct: 481 NMCFTLMEYTFLFLGPVLSRIVPAFYIPTKFFRMLLNKLARDFHNYPAVGGLVQTLMSYV 540

Query: 542 LGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSP 601
           +GGDSSNWVGV+G PHYNMNDMPG+SFRV  HLAQ+KH+ KF+MFDYG++S NM+ YGSP
Sbjct: 541 VGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIKHSGKFKMFDYGSSSANMDVYGSP 600

Query: 602 EPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTF 626
           EPLDLGE++GL D+PVDLVAG+KD+VIRP+MV+++Y++M+++GV+VS+NEFEYAHLDFTF
Sbjct: 601 EPLDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVMRDSGVDVSYNEFEYAHLDFTF 660

BLAST of Lag0024264 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 770.0 bits (1987), Expect = 1.5e-222
Identity = 384/613 (62.64%), Postives = 465/613 (75.86%), Query Frame = 0

Query: 71  MENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPLSQTSRGSRWSGSSSTPG 130
           M NGVSSFN+FIHELSVDSD SSL+YSSGE+       P +P SQ+SR S W+ +S++  
Sbjct: 1   MHNGVSSFNEFIHELSVDSDTSSLEYSSGEDS--DGPLPPSPSSQSSRLS-WASTSASSE 60

Query: 131 SPW------------------------------------------------PSIKRAHSH 190
           + W                                                P I + +S 
Sbjct: 61  NHWTEWITFILWWLMFPVRILLWIPQYIISLFYKRSSRIPASPRRHQHSSRPRISKTNSS 120

Query: 191 KDHNVVHRTTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAIRTLFRWLFSRG 250
           KDH+V +RTTDRRRGVIEDLHLA EI IE++F+  HKA H +LSP +A   L  W  S  
Sbjct: 121 KDHDVPNRTTDRRRGVIEDLHLAIEIGIEAIFDFFHKATHLLLSPSEAFAILLSWFSSSS 180

Query: 251 TWERDHDADV----IVPTATLGDDDPVPSERTY----TFHQSLNTDARTCQDVITELGYP 310
              +++  +V     V TATLGD DP P+ER        + S+NTD RTCQDVITELGYP
Sbjct: 181 RSPKENHGEVAYDETVQTATLGDTDPSPTERPVESPTRLYNSMNTDTRTCQDVITELGYP 240

Query: 311 YEAIRVVTNDGYVLLLERIPRRDARKALYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYD 370
           YEAIRV+T+DGYVL+LERIPRRDARKA++LQHG+ DSSMGWVSNGVVGSPAFAA+DQGYD
Sbjct: 241 YEAIRVITSDGYVLVLERIPRRDARKAVFLQHGVLDSSMGWVSNGVVGSPAFAAYDQGYD 300

Query: 371 VYLGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELA 430
           V+LGNFRGLVSR+H++KNISS+++W+YSINEH  EDIPAMIEKIHE+KT+ELKL QP + 
Sbjct: 301 VFLGNFRGLVSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTTELKLCQPNID 360

Query: 431 EETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTV 490
           EE + ++P+KLCA+CHS+GGA +LMYVITR+I+EKPHRLSRL+LLSPAGFH+D+   FT+
Sbjct: 361 EEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLILLSPAGFHEDSNLGFTI 420

Query: 491 IENLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTIVSYFLGGDSS 550
           +E + L ++P+LA  VP  YIPTRFFRMLLNKLARDFH+YPA+GGLVQT++SY +GGDSS
Sbjct: 421 VEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALGGLVQTLMSYVVGGDSS 480

Query: 551 NWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLG 610
           NWVGVLG PHYNMNDMP VSFRV  HLAQ+KH  KFRM+DYG+ S NME YGSPEPLDLG
Sbjct: 481 NWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEPLDLG 540

Query: 611 EYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKEAGVEVSFNEFEYAHLDFTFSHREEL 628
           E +   D+PVDLVAGR D+VIR +MVK++Y +M++A V+VSFNEFEYAHLDFTFSHREEL
Sbjct: 541 ESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHLDFTFSHREEL 600

BLAST of Lag0024264 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 86.3 bits (212), Expect = 9.8e-17
Identity = 92/379 (24.27%), Postives = 149/379 (39.31%), Query Frame = 0

Query: 244 CQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDA---------RKALYLQHGIFDSSMG 303
           C   +   GY  E   VVT DGY+L ++RIP   A         R+ + +QHGI    M 
Sbjct: 52  CASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMS 111

Query: 304 WVSNGVVGSPAFAAFDQGYDVYLGNFRGL-VSREHIDKNISSRQYWKYSINEHAMEDIPA 363
           W+ N    +      DQG+DV++GN RG   SR H   N S R +W ++ +E    D+PA
Sbjct: 112 WLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPA 171

Query: 364 MIEKIHEVKTSELKLSQPELAEETDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRL 423
           M + IH +                      K+  L HS+   G L+   +   +    ++
Sbjct: 172 MFDHIHGLTGQ-------------------KIHYLGHSL---GTLIGFASFSEKGLVDQV 231

Query: 424 SRLVLLSPAGFHDDAPFIFTVI--ENLLLLLAPILA--PFVPGLYIPTRFFRMLLNKLAR 483
               +LSP  +      +   I  +  L     IL    F P   +   F + +  K   
Sbjct: 232 RSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGI 291

Query: 484 DFHHYPAVGGLVQTIVSYFLGGDSSNWVGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKK 543
           D +           +VS   G +       +    +  N+    S +  +HLAQ    K+
Sbjct: 292 DCY----------DLVSVITGKNCCLNASTIDL--FLANEPQSTSTKNMIHLAQTVRDKE 351

Query: 544 FRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKE 603
            R ++YG++  N++ YG   P          ++P+    G  D +     V+      K 
Sbjct: 352 LRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKY 396

Query: 604 AGVEVSFNEF--EYAHLDF 607
             ++    +F  +YAH DF
Sbjct: 412 HDIDKMNVQFVKDYAHADF 396

BLAST of Lag0024264 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 75.9 bits (185), Expect = 1.3e-13
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 244 CQDVITELGYPYEAIRVVTNDGYVLLLERI----PRRDARKALYLQHGIFDSSMGWVSNG 303
           C D+I    Y      + T DGY+L L+R+    PR  +   + LQHG+F +   W  N 
Sbjct: 33  CADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGPPVLLQHGLFMAGDVWFLNS 92

Query: 304 VVGSPAFAAFDQGYDVYLGNFRGL-VSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKI 363
              S  F   D G+DV++GN RG   S  H+  + + +++W +S  + AM D+  MI+ +
Sbjct: 93  PKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYDLAEMIQYL 152

Query: 364 HEVKTSELKL 369
           + +  S++ L
Sbjct: 153 YSISNSKIFL 162

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023000889.10.0e+0094.60lipase member N isoform X2 [Cucurbita maxima][more]
XP_022923984.10.0e+0094.29lipase member N isoform X2 [Cucurbita moschata][more]
XP_023000888.10.0e+0089.04uncharacterized protein LOC111495192 isoform X1 [Cucurbita maxima][more]
XP_023519486.10.0e+0094.13lipase member N isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6584371.10.0e+0088.89Lipase member N, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019955.1 L... [more]
Match NameE-valueIdentityDescription
Q8K2A61.2e-2226.95Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1[more]
Q5VYY24.4e-2226.12Lipase member M OS=Homo sapiens OX=9606 GN=LIPM PE=2 SV=2[more]
P800351.7e-2128.78Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
Q3U4B41.7e-2126.79Lipase member N OS=Mus musculus OX=10090 GN=Lipn PE=2 SV=1[more]
O461081.2e-1926.63Lipase 3 OS=Drosophila melanogaster OX=7227 GN=Lip3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KL850.0e+0094.60lipase member N isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1[more]
A0A6J1E7W00.0e+0094.29lipase member N isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431539 PE=4 SV... [more]
A0A6J1KNX80.0e+0089.04uncharacterized protein LOC111495192 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EB220.0e+0088.74uncharacterized protein LOC111431539 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A1S3C3R60.0e+0087.26uncharacterized protein LOC103496491 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT1G73920.12.7e-25664.22alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.17.2e-25464.55alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.21.5e-22262.64alpha/beta-Hydrolases superfamily protein [more]
AT5G14180.19.8e-1724.27Myzus persicae-induced lipase 1 [more]
AT2G15230.11.3e-1331.54lipase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 245..299
e-value: 2.0E-12
score: 46.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 232..623
e-value: 5.3E-79
score: 267.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 242..620
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 92..147
NoneNo IPR availablePANTHERPTHR11005:SF134ALPHA/BETA HYDROLASE FAMILY PROTEINcoord: 9..93
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 138..626
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 9..93
NoneNo IPR availablePANTHERPTHR11005:SF134ALPHA/BETA HYDROLASE FAMILY PROTEINcoord: 138..626

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024264.1Lag0024264.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane