Lag0023277 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0023277
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr7: 46738394 .. 46741821 (+)
RNA-Seq ExpressionLag0023277
SyntenyLag0023277
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCTTGGCTCTCGTTTCTCCTTCATCCTTCGATTCATTCTCTTCTTCTGCCTTCAACAGACCCACAAATCCTCTCTCAACATTCGCTTCATCTCCCATATCGTGCTCATTCAAACCTTTCTGGCTTCTCCCATCACATTCCACTCACAAAATCGTTAAACGCCGGAAACCCATTAGAAATCAAACTGAATTTGACTTCAAAAGTAAAAATGGCAGTACCCCACTTGACGATCGGTGGTCTCTTCGCGGAACGACGGCCCTCGTTACCGGTGGCACTCGCGGAATCGGGTAATTTTTTTATATCTATATGAACAAATAGAATCGATTTTCGTTTTGTTCCTATGTTGTTGAGAATTTGGAATTGGTTTATGGTTTAGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCTAGAGTGCACACTTGTTCTCGGAATGAGAGTGAGCTGAGACAGTGTTTGAGGGACTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGTTCAGAGAGAGGAGCTAATGGAGATTGTGGCTCATACTTTTGATGGGAAGCTCAACATTCTTGTAAGGTGCACCCTTTCTTGTGCTTTGCTCTGCTTTAGATTAAGATGCATTGTACTTTGTTGGGTAGGTGCACCCTTTTTTGTGCTTTGCTCTGCTTTAGATTAAGCTGCATTTTACTTTGTTGGGTTACTTTCCTTTCAATCACACCTCCTACTGACATTAAAATTTAGAAATTATTGGTAGGAAGCTTCTACCTGCCGGTCTCATTACTCATTAGATGTCTAATATGAACAATATTTTCCTTTTCTCTTTTCTGCTTGCAATAGTTATGAAAGTGACAACATTGAATAGTTTTCTTGATAAAAATGATTTAGCTAAGACCTTGCCACAACTCACATGAATCAATGCCAAGAACTAAGTCAGGGAAAGCTACATGAAGCCTTAGCAAACATGTGTAGAACCATGAGGTGCAAACTGTAGGATGAAGAAAGCTTCTATTCAACATGGGCTAAGAGAAGCCTCCACACATTGAGGACTTGAAGACAAGAAAGCCAAATTCCAGAGACAACAAGCTTTGGAAACCCACACACGGCCCTTGCGCATTTTGAACTATCAATTGGGTTTTACTTTGAGGGTTTACTTAAAATCCTTGTTAGGATTCTAAATGGGTTTTTAAATGCCTTTAGGTTAGCCACTCACCCTCAAAGGCCTAGGAAGAAGTTTTTAGCTAGTATGCATCTTCTCAATTTTATTATTTTCCTTTTCTAGTTGTACAAGCTAGTTCCAGGGATTTATTATCATGAGTTTTTCCCATGCCCCATTGCCGTTCACTTAAGGCTTTGTAAGGCCAGGCCTAATCCATACTTGTAAAATGGTTAGATATCATGATGATGAAATGAATTTGAGAGGTTTCTCTTTTGCTCATTGTTGAGAGCATGAGTGTATCTGCAATCCTAACATAGTTTTCACTTATAAACATTCAAATTAGGCTGGTCATCAAATCTGTGGAGTGTTTCAAACTCGGGACTAGGATTTTTTTGTCCTTGCTTCTTGATATTTGACCACCAAAGGCAACCCCATCCTAACTTTCCTTTGAGTTGAAACCTTGTGTGTGTGAAGGGTTTTGGTGCTTGTGTTCCAAGATCCCCACCAAAGGGTTTCTAAGCCAATTTATCAACGTCTCTCTTATCATTTGATATCAGAGTTTTTGATTCGTATCAATTATCTAAGAGGGGTATGGCCAACGTTTTCAAAGACCAAAGGTGGTTTTGGGACTTTTTCTCATGAAGAAATGAGACTGGAGTCTTGAGCCAGCATGAGGCGCAAAGACTCAAGTAAGCTTCTTCACATAGATACATAAACCTCTTATAATCCAAAGTACGGTAAGTAAGACTAAGAGGTTAAAAATTTTAGTCTCACACTTGAGGTGAGTTTAAGGTGTAAGCCTCAAAAGCCTTATAAAACATTAAACGAGACAAAAGTTTGAGGGCTTTATTTTGAATTTTGATCATTTATCTAAAAGATCTCTAGTTTACTACTTTGCTTAAATTAAATAAAAAAAATGTAGTTAAAAGAAAAGAAAATGAAGATCCCAAATATCTTAGAACTTTGCTCAACTTCTTTAAAGATTCTTCAATGAAATCCTTTGGTGGATCTTCACTACGTAAGTTTTTTTTTTCTGACTGAAATTTAATTGTTTGGATATTTGTTGCTTTTTGGCATATTGAGTTGTACTTCCCTTCTGTAATATGAAGTTTACGAAAGTGAGGTTAATTTTGAATTTTCTAATTTGCCTCGTTTTGATAATCATTTGGTTTTTGGTTTTTGAAAATTAAGCTTGAAAACATTACTTCCACCTCTAAGTTTCCTGGTTTTATTATCTACCTTCTCCCTATGTTTTCAAAAACCAAGTCAAGTTTTGAAAACTAAAAAAAGTAGTTTTCAAGAACTTGTTTTTGTTTTTGGAATTTGGCTAGGAATTCAATGTGTCATTGACAAAGACGAAAACCAACCTAAGGAAATTGAGAGAAAATAAGTTTAATTTTCAAAAACCAAATGGTTATCAAATGAAGCTTTAGTTTTTCTATGAACTAATCCTCTAGCATGATATGGGCAAATTCTAAATTTTAGAATAAAGAAGCAAATTGATTTTCATCATTCTTTCAATCCTTATGGACTATGAGCACCATTTCTACTACCATGTAGGTAAATAATGTGGGTAGAAATATACGAAAGCCAACAGCAGAGTTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTGGAATCAGTCTTCCATTTATCTCAACTCGCTTATCCTCTTTTAAAAGCATCGGGCGAGGGAAGCATCGTGTTCATGTCCTCAGTCTCAAGTTTTGTCTCTCTAAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGTGATCAATTGTACCAGTGGTTTCTAAATATTTTGGATGCATACATTCGTTTCCTTGTTGGAACATTTCCCAGCAATGTGTAATGATTCTTTTCCAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAGTGGGCAAAAGACAACATAAGGAGCAATGCAGTCGCCCCGTGGTACATCAAAACGTCGATGGTGGAACAAGTAAATCTCGAGTAACACCATTCTGTTTTAACTTGTGAAGAATCACTCATAATGTTTGTTTCACTGAACTTATGCCATATCTTTTCTTCTAGGTGCTGAGTAACAAAGCATATGTAGAAGAGGTATATTCTCGAACCCCGCTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGACAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAGATAA

mRNA sequence

ATGCTCTTGGCTCTCGTTTCTCCTTCATCCTTCGATTCATTCTCTTCTTCTGCCTTCAACAGACCCACAAATCCTCTCTCAACATTCGCTTCATCTCCCATATCGTGCTCATTCAAACCTTTCTGGCTTCTCCCATCACATTCCACTCACAAAATCGTTAAACGCCGGAAACCCATTAGAAATCAAACTGAATTTGACTTCAAAAGTAAAAATGGCAGTACCCCACTTGACGATCGGTGGTCTCTTCGCGGAACGACGGCCCTCGTTACCGGTGGCACTCGCGGAATCGGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCTAGAGTGCACACTTGTTCTCGGAATGAGAGTGAGCTGAGACAGTGTTTGAGGGACTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGTTCAGAGAGAGGAGCTAATGGAGATTGTGGCTCATACTTTTGATGGGAAGCTCAACATTCTTGTAAATAATGTGGGTAGAAATATACGAAAGCCAACAGCAGAGTTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTGGAATCAGTCTTCCATTTATCTCAACTCGCTTATCCTCTTTTAAAAGCATCGGGCGAGGGAAGCATCGTGTTCATGTCCTCAGTCTCAAGTTTTGTCTCTCTAAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAGTGGGCAAAAGACAACATAAGGAGCAATGCAGTCGCCCCGTGGTACATCAAAACGTCGATGGTGGAACAAGTGCTGAGTAACAAAGCATATGTAGAAGAGGTATATTCTCGAACCCCGCTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGACAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAGATAA

Coding sequence (CDS)

ATGCTCTTGGCTCTCGTTTCTCCTTCATCCTTCGATTCATTCTCTTCTTCTGCCTTCAACAGACCCACAAATCCTCTCTCAACATTCGCTTCATCTCCCATATCGTGCTCATTCAAACCTTTCTGGCTTCTCCCATCACATTCCACTCACAAAATCGTTAAACGCCGGAAACCCATTAGAAATCAAACTGAATTTGACTTCAAAAGTAAAAATGGCAGTACCCCACTTGACGATCGGTGGTCTCTTCGCGGAACGACGGCCCTCGTTACCGGTGGCACTCGCGGAATCGGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCTAGAGTGCACACTTGTTCTCGGAATGAGAGTGAGCTGAGACAGTGTTTGAGGGACTGGAAGGATTTGGAATTTGAGGTTTCTGGGTCAGTTTGTGATGTGTCTGTTCGAGTTCAGAGAGAGGAGCTAATGGAGATTGTGGCTCATACTTTTGATGGGAAGCTCAACATTCTTGTAAATAATGTGGGTAGAAATATACGAAAGCCAACAGCAGAGTTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTGGAATCAGTCTTCCATTTATCTCAACTCGCTTATCCTCTTTTAAAAGCATCGGGCGAGGGAAGCATCGTGTTCATGTCCTCAGTCTCAAGTTTTGTCTCTCTAAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAGTGGGCAAAAGACAACATAAGGAGCAATGCAGTCGCCCCGTGGTACATCAAAACGTCGATGGTGGAACAAGTGCTGAGTAACAAAGCATATGTAGAAGAGGTATATTCTCGAACCCCGCTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGACAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAGATAA

Protein sequence

MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIRNQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR
Homology
BLAST of Lag0023277 vs. NCBI nr
Match: XP_038893799.1 (tropinone reductase homolog At5g06060-like [Benincasa hispida])

HSP 1 Score: 600.9 bits (1548), Expect = 6.7e-168
Identity = 307/335 (91.64%), Postives = 317/335 (94.63%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTN-PLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPI 60
           MLLALVSPSSF SFSSS FNRPTN PLSTF+S PISCSFKPFWL PSHS+HK+VKR KPI
Sbjct: 1   MLLALVSPSSFYSFSSSTFNRPTNPPLSTFSSFPISCSFKPFWLSPSHSSHKLVKRLKPI 60

Query: 61  RNQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNES 120
            NQTE DFK K GSTP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE 
Sbjct: 61  TNQTESDFKIKYGSTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG 120

Query: 121 ELRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTA 180
           ELRQCLRDW +L+FEVSGSVCDVSVR QR+ELMEIVA TFDGKL+ILVNNVGRNIRKPT 
Sbjct: 121 ELRQCLRDWNNLKFEVSGSVCDVSVRAQRDELMEIVADTFDGKLSILVNNVGRNIRKPTT 180

Query: 181 EFTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKG 240
           EFTDEEFSFLMKTNVE+VFHLSQLAYPLLK+SGEGSIVFMSSVSS VSLKSMSVQGATKG
Sbjct: 181 EFTDEEFSFLMKTNVEAVFHLSQLAYPLLKSSGEGSIVFMSSVSSLVSLKSMSVQGATKG 240

Query: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSE 300
           AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSE
Sbjct: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSE 300

Query: 301 VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR
Sbjct: 301 VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335

BLAST of Lag0023277 vs. NCBI nr
Match: XP_031741141.1 (tropinone reductase homolog At5g06060 [Cucumis sativus])

HSP 1 Score: 581.3 bits (1497), Expect = 5.5e-162
Identity = 298/334 (89.22%), Postives = 307/334 (91.92%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           MLL LVSPSSF SFSSS FN+  +P STF+S PI CSF  F L PSHSTHK++KR  PIR
Sbjct: 1   MLLVLVSPSSFPSFSSSTFNK--SPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQTE DF SK G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           LRQCLR WKDLEFEVSGSVCDVSVR QREELME   +TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLAYPLLK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAY EEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

BLAST of Lag0023277 vs. NCBI nr
Match: XP_008457446.1 (PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 580.1 bits (1494), Expect = 1.2e-161
Identity = 299/334 (89.52%), Postives = 307/334 (91.92%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           MLLALVSPSSF SFSSS FN+  +  STF+S PI CS  PF L PSHSTHK+ KR  PIR
Sbjct: 1   MLLALVSPSSFPSFSSSTFNK--SHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQTE DFKSK G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           LRQCLR WKDLEFEVSGSVCDVSVR QREELME   +TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLAYPLLK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

BLAST of Lag0023277 vs. NCBI nr
Match: KAG6607269.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 578.6 bits (1490), Expect = 3.6e-161
Identity = 299/333 (89.79%), Postives = 307/333 (92.19%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           M LALVSPS  DSFSSS FN     LSTF+SSPIS SFKPFWL  S+STHK+VKR K IR
Sbjct: 1   MFLALVSPSPSDSFSSSTFNPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQ E DFK+KNGSTP D RWSL GTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNE+E
Sbjct: 61  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           L+QCLRDWKDLEFEVSGSVCDVSVR QREELM+ VA TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LKASG GSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 334
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

BLAST of Lag0023277 vs. NCBI nr
Match: XP_022998606.1 (tropinone reductase homolog At5g06060-like [Cucurbita maxima])

HSP 1 Score: 577.8 bits (1488), Expect = 6.1e-161
Identity = 298/333 (89.49%), Postives = 307/333 (92.19%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           M LALVSPS FDS SSS FN      STF+SSPIS S KPFWL  S+STHK+VKR K IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQ E DFK+KNGSTP D RWSL GTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           L+QCLRDWKDLEFEVSGSVCDVSVR QREELM+ VA TF+GKLNILVNNVGRNIRKPT E
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTN+ESVFHLSQLA+P LKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNIESVFHLSQLAHPFLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 334
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

BLAST of Lag0023277 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-106
Identity = 210/313 (67.09%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 23  TNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIRNQTEFDFKSKNG-STPLDDRWS 82
           TNP         S S KP   L   S  +I  + K   +     + S++  +    +RWS
Sbjct: 11  TNPPQNLHFISSSSSLKPHLCL---SFKRINPKHKSSSSSVFVPYASQSSIAITSKERWS 70

Query: 83  LRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEVSGSVCD 142
           L G +ALVTGGTRGIGRAIVEEL G GA VHTC+RNE EL  CL DW    F V+GSVCD
Sbjct: 71  LNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGSVCD 130

Query: 143 VSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVESVFHLS 202
           VS R QRE LME V+  F+GKL+ILVNNVG NIRKP  EFT  EFS LM TN ESVFHL 
Sbjct: 131 VSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLC 190

Query: 203 QLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNA 262
           QLAYPLL+ S  GS+VF+SSVS FVSLK+MSVQ +TKGAINQLT+ LACEWAKDNIR NA
Sbjct: 191 QLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINA 250

Query: 263 VAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIG 322
           VAPWYIKTSMVEQVLSNK Y+EEVYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ 
Sbjct: 251 VAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILC 310

Query: 323 VDGGMSVNGFDPR 335
           VDGGMS+NGF PR
Sbjct: 311 VDGGMSINGFFPR 320

BLAST of Lag0023277 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-99
Identity = 179/255 (70.20%), Postives = 208/255 (81.57%), Query Frame = 0

Query: 77  DDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEVS 136
           D RWSL G TALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL DWK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 137 GSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVES 196
           GSVCD SVR QRE+L++  +  F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 197 VFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 256
            FHLSQ+A+PLLKASG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 257 IRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYIT 316
           IR+N VAPWYIKTS+VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 317 GQIIGVDGGMSVNGF 332
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

BLAST of Lag0023277 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 7.1e-88
Identity = 159/256 (62.11%), Postives = 204/256 (79.69%), Query Frame = 0

Query: 76  LDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEV 135
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E++L++ LR+W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 136 SGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVE 195
           + S+CDVS+R QRE+LME V+  F GKLNILVNNVG  + KPT E+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 196 SVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 255
           S FH+SQLA+PLLKASG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 256 NIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 315
           NIR+NA+ PW I T ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 316 TGQIIGVDGGMSVNGF 332
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

BLAST of Lag0023277 vs. ExPASy Swiss-Prot
Match: A0A1C9II22 (Noroxomaritidine/norcraugsodine reductase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=NR PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 155/255 (60.78%), Postives = 203/255 (79.61%), Query Frame = 0

Query: 76  LDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEV 135
           L+ RWSL GTTALVTGGT+GIG AIVEELVGFGARV+TCSRNE+ELR+CL++W++L+++V
Sbjct: 3   LEKRWSLEGTTALVTGGTKGIGHAIVEELVGFGARVYTCSRNEAELRKCLQEWENLKYDV 62

Query: 136 SGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVE 195
           +GSVCDVS R +RE+L E V+  F+GKLNIL+NN G  + KP   FT E+FSFL+  N+E
Sbjct: 63  TGSVCDVSSRTEREKLAEEVSSVFNGKLNILINNAGGYVNKPIDGFTAEDFSFLVAVNLE 122

Query: 196 SVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 255
           S FHL QLA+P+LKASG GSIV +SS  + +++   S+  +TKGAINQLT+ LACEWAKD
Sbjct: 123 SAFHLCQLAHPMLKASGTGSIVHISSCCAQIAIPGHSIYSSTKGAINQLTRNLACEWAKD 182

Query: 256 NIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 315
           NIR+N++AP  I+T   E  + +K  ++   SR P  R+GEP EV+SL AFLC+P++SYI
Sbjct: 183 NIRTNSIAPGAIRTPGTESFVIDKDALDREVSRVPFGRIGEPEEVASLAAFLCMPSASYI 242

Query: 316 TGQIIGVDGGMSVNG 331
           TGQ+I VDGG ++NG
Sbjct: 243 TGQVICVDGGRTING 257

BLAST of Lag0023277 vs. ExPASy Swiss-Prot
Match: A0A1A9TAK5 (Noroxomaritidine/norcraugsodine reductase OS=Narcissus pseudonarcissus OX=39639 GN=NR PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-86
Identity = 155/255 (60.78%), Postives = 203/255 (79.61%), Query Frame = 0

Query: 76  LDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEV 135
           L+ RWSL GTTALVTGGT+GIG AIVEELVGFGARV+TCSRNE+ELR+CL++W++L+++V
Sbjct: 3   LEKRWSLEGTTALVTGGTKGIGHAIVEELVGFGARVYTCSRNEAELRKCLQEWENLKYDV 62

Query: 136 SGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVE 195
           +GSVCDVS R +RE+L E V+  F+GKLNIL+NN G  + KP   FT E+FSFL+  N+E
Sbjct: 63  TGSVCDVSSRTEREKLAEEVSSVFNGKLNILINNAGGYVNKPIDGFTAEDFSFLVAVNLE 122

Query: 196 SVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 255
           S FHL QLA+P+LKASG GSIV +SS  + +++   S+  +TKGAINQLT+ LACEWAKD
Sbjct: 123 SAFHLCQLAHPMLKASGTGSIVHISSCCAQIAIPGHSIYSSTKGAINQLTRNLACEWAKD 182

Query: 256 NIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 315
           NIR+N++AP  I+T   E  + +K  ++   SR P  R+GEP EV+SL AFLC+P++SYI
Sbjct: 183 NIRTNSIAPGAIRTPGTESFVIDKDALDREVSRVPFGRIGEPEEVASLAAFLCMPSASYI 242

Query: 316 TGQIIGVDGGMSVNG 331
           TGQ+I VDGG ++NG
Sbjct: 243 TGQVICVDGGRTING 257

BLAST of Lag0023277 vs. ExPASy TrEMBL
Match: A0A5D3BGS1 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001920 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.9e-162
Identity = 299/334 (89.52%), Postives = 307/334 (91.92%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           MLLALVSPSSF SFSSS FN+  +  STF+S PI CS  PF L PSHSTHK+ KR  PIR
Sbjct: 1   MLLALVSPSSFPSFSSSTFNK--SHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQTE DFKSK G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           LRQCLR WKDLEFEVSGSVCDVSVR QREELME   +TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLAYPLLK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

BLAST of Lag0023277 vs. ExPASy TrEMBL
Match: A0A1S3C5I5 (tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE=4 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.9e-162
Identity = 299/334 (89.52%), Postives = 307/334 (91.92%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           MLLALVSPSSF SFSSS FN+  +  STF+S PI CS  PF L PSHSTHK+ KR  PIR
Sbjct: 1   MLLALVSPSSFPSFSSSTFNK--SHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQTE DFKSK G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQTESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           LRQCLR WKDLEFEVSGSVCDVSVR QREELME   +TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LRQCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLAYPLLK+SGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

BLAST of Lag0023277 vs. ExPASy TrEMBL
Match: A0A6J1KAL5 (tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111493193 PE=4 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.9e-161
Identity = 298/333 (89.49%), Postives = 307/333 (92.19%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           M LALVSPS FDS SSS FN      STF+SSPIS S KPFWL  S+STHK+VKR K IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQ E DFK+KNGSTP D RWSL GTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           L+QCLRDWKDLEFEVSGSVCDVSVR QREELM+ VA TF+GKLNILVNNVGRNIRKPT E
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTN+ESVFHLSQLA+P LKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNIESVFHLSQLAHPFLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 334
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

BLAST of Lag0023277 vs. ExPASy TrEMBL
Match: A0A6J1GAE7 (tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC111452252 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 297/333 (89.19%), Postives = 306/333 (91.89%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           M LALVSPS FDS SSS F+     LSTF+SSPIS SFKPFWL  S+STHK+VKR K IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFSPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQ   DFK+KNGSTP D RWSL GTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNE+E
Sbjct: 61  NQAASDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           L+QCLRDWKDLEFEVSGSVCDVSVR QREELM+ VA TFDGKLNILVNNVGRNIRKPT E
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LKASG GSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 334
           SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

BLAST of Lag0023277 vs. ExPASy TrEMBL
Match: A0A6J1CL23 (tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC111012129 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.5e-157
Identity = 293/334 (87.72%), Postives = 304/334 (91.02%), Query Frame = 0

Query: 1   MLLALVSPSSFDSFSSSAFNRPTNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIR 60
           MLLALVSP+SFDSFSS  F  PTNP    +   ISCSF PF L PS+ST KI+ R KPI 
Sbjct: 1   MLLALVSPTSFDSFSSFTFITPTNP--PLSPISISCSFNPFGLPPSYSTRKILHRLKPIT 60

Query: 61  NQTEFDFKSKNGSTPLDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120
           NQT+ D KS NG+TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRN SE
Sbjct: 61  NQTQPDLKSMNGTTPIDDRWSLRGATALVTGGTRGIGRAIVEELVGFGARVHTCSRNLSE 120

Query: 121 LRQCLRDWKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAE 180
           L+QCLRDWK+LEF VSGSVCDVSVR QREELME VA  FDGKLNILVNNVGRNIRKPTAE
Sbjct: 121 LQQCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAANFDGKLNILVNNVGRNIRKPTAE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240
           FT+EEFSFLMKTNVESVFHLSQLAYPLLKASG GSIVFMSSVSSFVSLKSMSVQGATKGA
Sbjct: 181 FTEEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK YVEEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKVYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335
           SSLVAFLCLPASSYITGQIIGVDGGMS+NGFDPR
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSINGFDPR 332

BLAST of Lag0023277 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 8.3e-108
Identity = 210/313 (67.09%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 23  TNPLSTFASSPISCSFKPFWLLPSHSTHKIVKRRKPIRNQTEFDFKSKNG-STPLDDRWS 82
           TNP         S S KP   L   S  +I  + K   +     + S++  +    +RWS
Sbjct: 11  TNPPQNLHFISSSSSLKPHLCL---SFKRINPKHKSSSSSVFVPYASQSSIAITSKERWS 70

Query: 83  LRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEVSGSVCD 142
           L G +ALVTGGTRGIGRAIVEEL G GA VHTC+RNE EL  CL DW    F V+GSVCD
Sbjct: 71  LNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGSVCD 130

Query: 143 VSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVESVFHLS 202
           VS R QRE LME V+  F+GKL+ILVNNVG NIRKP  EFT  EFS LM TN ESVFHL 
Sbjct: 131 VSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLC 190

Query: 203 QLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNA 262
           QLAYPLL+ S  GS+VF+SSVS FVSLK+MSVQ +TKGAINQLT+ LACEWAKDNIR NA
Sbjct: 191 QLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINA 250

Query: 263 VAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIG 322
           VAPWYIKTSMVEQVLSNK Y+EEVYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ 
Sbjct: 251 VAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILC 310

Query: 323 VDGGMSVNGFDPR 335
           VDGGMS+NGF PR
Sbjct: 311 VDGGMSINGFFPR 320

BLAST of Lag0023277 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 9.9e-101
Identity = 179/255 (70.20%), Postives = 208/255 (81.57%), Query Frame = 0

Query: 77  DDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEVS 136
           D RWSL G TALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL DWK     VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 137 GSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVES 196
           GSVCD SVR QRE+L++  +  F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 197 VFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 256
            FHLSQ+A+PLLKASG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 257 IRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYIT 316
           IR+N VAPWYIKTS+VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 317 GQIIGVDGGMSVNGF 332
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

BLAST of Lag0023277 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 5.1e-89
Identity = 159/256 (62.11%), Postives = 204/256 (79.69%), Query Frame = 0

Query: 76  LDDRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRDWKDLEFEV 135
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E++L++ LR+W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 136 SGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFSFLMKTNVE 195
           + S+CDVS+R QRE+LME V+  F GKLNILVNNVG  + KPT E+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 196 SVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 255
           S FH+SQLA+PLLKASG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 256 NIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYI 315
           NIR+NA+ PW I T ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 316 TGQIIGVDGGMSVNGF 332
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

BLAST of Lag0023277 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 3.6e-87
Identity = 163/264 (61.74%), Postives = 206/264 (78.03%), Query Frame = 0

Query: 69  SKNGSTPLD-DRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRD 128
           +K G +  D  RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E++L++ LR 
Sbjct: 2   AKTGESLRDKPRWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRK 61

Query: 129 WKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFS 188
           W+   F+V+ SVCDVS R +RE+LME V+  F+GKLNILVNNVG  I KPT + T E+FS
Sbjct: 62  WQAKGFQVTTSVCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFS 121

Query: 189 FLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKY 248
           F M TN+ES FHLSQLA+PLLKASG GSIV +SSVS  V +   S+ G +KGA+NQL + 
Sbjct: 122 FTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRN 181

Query: 249 LACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFL 308
           LACEWA DNIR+N+V PW+I+T +V + LSN+ + +EV SR P+ R+GE +EVSSLVAFL
Sbjct: 182 LACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFL 241

Query: 309 CLPASSYITGQIIGVDGGMSVNGF 332
           CLPA+SYITGQ I VDGG +VNGF
Sbjct: 242 CLPAASYITGQTICVDGGFTVNGF 265

BLAST of Lag0023277 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.0e-86
Identity = 160/264 (60.61%), Postives = 204/264 (77.27%), Query Frame = 0

Query: 69  SKNGSTPLD-DRWSLRGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESELRQCLRD 128
           +K G +  D  +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E++L++ LR+
Sbjct: 2   AKRGESLRDKPKWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLRE 61

Query: 129 WKDLEFEVSGSVCDVSVRVQREELMEIVAHTFDGKLNILVNNVGRNIRKPTAEFTDEEFS 188
           W+    +V+ SVCDVS R QRE+LME V+  F GKL+ILV NVG  + KPT E T EEFS
Sbjct: 62  WQAKGLQVTTSVCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFS 121

Query: 189 FLMKTNVESVFHLSQLAYPLLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKY 248
           F++ TN+ES FH SQLA+PLLKASG G+IV MSSV+  V+L + S+ GATKGA+NQL + 
Sbjct: 122 FIIATNLESTFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARN 181

Query: 249 LACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFL 308
           LACEWA DNIR+N+V PW+I T   +  L +K   E+V S TPLRR+GE +EVSSLVAFL
Sbjct: 182 LACEWASDNIRANSVCPWFITTPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFL 241

Query: 309 CLPASSYITGQIIGVDGGMSVNGF 332
           CLPA+SYITGQ I VDGG ++NGF
Sbjct: 242 CLPAASYITGQTICVDGGFTINGF 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893799.16.7e-16891.64tropinone reductase homolog At5g06060-like [Benincasa hispida][more]
XP_031741141.15.5e-16289.22tropinone reductase homolog At5g06060 [Cucumis sativus][more]
XP_008457446.11.2e-16189.52PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 trop... [more]
KAG6607269.13.6e-16189.79Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022998606.16.1e-16189.49tropinone reductase homolog At5g06060-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZW121.2e-10667.09Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9LHT01.4e-9970.20Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW137.1e-8862.11Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
A0A1C9II221.3e-8660.78Noroxomaritidine/norcraugsodine reductase OS=Narcissus aff. pseudonarcissus MK-2... [more]
A0A1A9TAK51.3e-8660.78Noroxomaritidine/norcraugsodine reductase OS=Narcissus pseudonarcissus OX=39639 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BGS15.9e-16289.52Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C5I55.9e-16289.52tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE... [more]
A0A6J1KAL52.9e-16189.49tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GAE71.9e-16089.19tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1CL231.5e-15787.72tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G29260.18.3e-10867.09NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G06060.19.9e-10170.20NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.25.1e-8962.11NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.13.6e-8761.74NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29370.14.0e-8660.61NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 86..103
score: 51.89
coord: 290..310
score: 34.57
coord: 234..253
score: 36.93
coord: 255..272
score: 39.59
coord: 161..172
score: 50.8
coord: 208..224
score: 31.08
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 161..172
score: 43.41
coord: 234..253
score: 31.56
coord: 214..222
score: 48.0
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 93..327
e-value: 2.0E-58
score: 197.7
NoneNo IPR availableGENE3D3.40.50.720coord: 67..331
e-value: 3.0E-79
score: 267.9
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 82..331
e-value: 8.7E-21
score: 72.0
NoneNo IPR availablePANTHERPTHR42898:SF2TROPINONE REDUCTASE-LIKE PROTEIN, CHLOROPLASTICcoord: 63..334
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 63..334
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 84..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0023277.1Lag0023277.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity