Lag0023014 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0023014
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
Locationchr7: 42905394 .. 42907288 (+)
RNA-Seq ExpressionLag0023014
SyntenyLag0023014
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATCAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTTATAATGACCTTCGCCGGCGGATTCTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACAAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATCGTCACCGGCCTTCTGTCCGAGGTGGCGTCGACTTGGGTCCTTCTCGTCGTCTACGCCGCCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCGCCAACCCGACCCTTGGCTACTTTTGCTTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGGTAAAGTCGATCTTTTATTACAAGTTTAGTCTTTGAATTTTTGGATTCTATTGATTAGACCCCTGAACTTTAAAAGGTCTCTGACTTATTGTATTTGAAAAATTATGTAATATTTCCAGTGGTGTTAGTGACGTGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGTCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCTCAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCATTAGCCGACACATTCGTCGTGATTGCAATCGTGGGTTTGCTTGTTCTTCCATTGTTTATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGGCCAAAGTTACCAGAATCCAGTCGACGCCTCAAACGCCCTTAACCTTAGCTCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCCAACATTTTTGACAAACCCGAAAGAGGAGAAGACTACACCATTCTTCAAGCCATTCTCAGCATCGACATGCTCATCTTGTACCTCACCATGATAATCGGTATTGGCTCCAGCTTCACCGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAACGCTACAAAACCGAAACCATCAATGCATTCATCTCTGTGCTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTGGAGAAGTACCAATTCCCCCGGCCGTTGATGCTCACTTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGCTCGCTCTTCATTGCATCCATCGTAATTGGGTTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTAAAACACTACTCACAGTTGTACAATTTTGGGCAATTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTGTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATGCTTTCACATATTTGACAGCGCTGACGTTGGTAGGAGCTGGGATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGACCTCCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

mRNA sequence

ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATCAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTTATAATGACCTTCGCCGGCGGATTCTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACAAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATCGTCACCGGCCTTCTGTCCGAGGTGGCGTCGACTTGGGTCCTTCTCGTCGTCTACGCCGCCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCGCCAACCCGACCCTTGGCTACTTTTGCTTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGTGGTGTTAGTGACGTGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGTCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCTCAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCATTAGCCGACACATTCGTCGTGATTGCAATCGTGGGTTTGCTTGTTCTTCCATTGTTTATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGGCCAAAGTTACCAGAATCCAGTCGACGCCTCAAACGCCCTTAACCTTAGCTCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCCAACATTTTTGACAAACCCGAAAGAGGAGAAGACTACACCATTCTTCAAGCCATTCTCAGCATCGACATGCTCATCTTGTACCTCACCATGATAATCGGTATTGGCTCCAGCTTCACCGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAACGCTACAAAACCGAAACCATCAATGCATTCATCTCTGTGCTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTGGAGAAGTACCAATTCCCCCGGCCGTTGATGCTCACTTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGCTCGCTCTTCATTGCATCCATCGTAATTGGGTTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTAAAACACTACTCACAGTTGTACAATTTTGGGCAATTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTGTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATGCTTTCACATATTTGACAGCGCTGACGTTGGTAGGAGCTGGGATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGACCTCCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

Coding sequence (CDS)

ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATCAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTTATAATGACCTTCGCCGGCGGATTCTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACAAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATCGTCACCGGCCTTCTGTCCGAGGTGGCGTCGACTTGGGTCCTTCTCGTCGTCTACGCCGCCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCGCCAACCCGACCCTTGGCTACTTTTGCTTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGTGGTGTTAGTGACGTGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGTCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCTCAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCATTAGCCGACACATTCGTCGTGATTGCAATCGTGGGTTTGCTTGTTCTTCCATTGTTTATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGGCCAAAGTTACCAGAATCCAGTCGACGCCTCAAACGCCCTTAACCTTAGCTCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCCAACATTTTTGACAAACCCGAAAGAGGAGAAGACTACACCATTCTTCAAGCCATTCTCAGCATCGACATGCTCATCTTGTACCTCACCATGATAATCGGTATTGGCTCCAGCTTCACCGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAACGCTACAAAACCGAAACCATCAATGCATTCATCTCTGTGCTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTGGAGAAGTACCAATTCCCCCGGCCGTTGATGCTCACTTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGCTCGCTCTTCATTGCATCCATCGTAATTGGGTTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTAAAACACTACTCACAGTTGTACAATTTTGGGCAATTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTGTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATGCTTTCACATATTTGACAGCGCTGACGTTGGTAGGAGCTGGGATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGACCTCCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

Protein sequence

MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTKS
Homology
BLAST of Lag0023014 vs. NCBI nr
Match: XP_004147304.1 (uncharacterized protein LOC101203173 [Cucumis sativus] >KAE8652743.1 hypothetical protein Csa_013832 [Cucumis sativus])

HSP 1 Score: 771.2 bits (1990), Expect = 6.6e-219
Identity = 399/595 (67.06%), Postives = 482/595 (81.01%), Query Frame = 0

Query: 1   MIVSTTC---SNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIR 60
           M VSTT    +NNGG R  E+ SFA QV+LGRWFA+F SFL+MT AGGFYLF YFS DI+
Sbjct: 1   MAVSTTLPVGNNNGGTRSVEISSFATQVVLGRWFALFASFLVMTGAGGFYLFAYFSKDIK 60

Query: 61  AKLNCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTG 120
             L CDQ++LNKIGFYKDLGSN+GI++G ++EVA  W LL++ +A NFIGYFK+W+GV G
Sbjct: 61  ETLKCDQTTLNKIGFYKDLGSNIGIISGFMAEVAPPWSLLLLASAVNFIGYFKIWEGVVG 120

Query: 121 QVANPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQ 180
           +V NPT+ YFCFY+ + GNSQ + NTVVLVTCVKNFPERRGV+LGLLKGF+GIG AVL Q
Sbjct: 121 RVVNPTVEYFCFYITVGGNSQILANTVVLVTCVKNFPERRGVILGLLKGFLGIGGAVLTQ 180

Query: 181 LFVAIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALL 240
           +  AIYG +TK++IL IAWFP+LITL+F F+IREI+VVKHPNEFRVF  FL++S+IL   
Sbjct: 181 IHYAIYGHETKSIILLIAWFPSLITLLFAFTIREIRVVKHPNEFRVFFHFLFVSLILPFF 240

Query: 241 LSVLIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQ 300
           L +LI  Q RV FD LA TFVV+AI+GLL+ PLFI+IREE+VQWNL K+ ++ + Q+  Q
Sbjct: 241 LFILIILQGRVHFDQLAYTFVVVAIMGLLLTPLFIAIREELVQWNLTKITQLVKSQTITQ 300

Query: 301 TPLT-LAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGS 360
             LT ++P + K        S+F NIFDKPERGEDYT LQA++SIDM ILYLTMIIGIGS
Sbjct: 301 KRLTSISPPTPKT------TSFFENIFDKPERGEDYTFLQAVMSIDMFILYLTMIIGIGS 360

Query: 361 SFTAVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLT 420
           SFTA+DNL QIGESQRY TE+I+  IS+ SI NF GRIFSGF SEILLEK++FPRPLMLT
Sbjct: 361 SFTAMDNLAQIGESQRYSTESIDLIISMASIFNFLGRIFSGFASEILLEKFKFPRPLMLT 420

Query: 421 FTLLVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQ 480
           FTLLVSCIG++LVAFPFH SL++ASI+IGF LGSQIPLYFA+ISEIFGLKHYS LYNFGQ
Sbjct: 421 FTLLVSCIGNILVAFPFHHSLYVASILIGFCLGSQIPLYFAMISEIFGLKHYSLLYNFGQ 480

Query: 481 LSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISL 540
           LSCP+G+Y+LNVLVAG  YD+E + +  +S    C G+ CYRN+F  LT ++LVGA ISL
Sbjct: 481 LSCPVGSYILNVLVAGRFYDEEAKTINGNSIYLTCKGEFCYRNSFAILTGMSLVGAVISL 540

Query: 541 ILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTKS 592
           ILVKRT  FY+GDIY+KFREDM+SLK+EVELY +  K    SV  +  Q+TDT S
Sbjct: 541 ILVKRTNEFYKGDIYRKFREDMDSLKSEVELYRIDTKSEIDSV-NVCRQTTDTSS 588

BLAST of Lag0023014 vs. NCBI nr
Match: XP_038895749.1 (uncharacterized protein LOC120083913 [Benincasa hispida])

HSP 1 Score: 750.4 bits (1936), Expect = 1.2e-212
Identity = 383/574 (66.72%), Postives = 471/574 (82.06%), Query Frame = 0

Query: 1   MIVST-TCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAK 60
           M+VST   SNNGG+   E  SFARQV++GRWF++F SFL+MT AG F+LF +FS DI+  
Sbjct: 1   MVVSTIPVSNNGGMGTAEFLSFARQVVVGRWFSLFASFLVMTGAGSFFLFPHFSKDIKET 60

Query: 61  LNCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQV 120
           L CDQ++LNKIGFYKDLGSNVGI++ LL  +  +W LLV+ +A NFIG+FK+W+ VTG+V
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGIISALLGGILPSWCLLVLSSAVNFIGHFKIWEAVTGKV 120

Query: 121 ANPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLF 180
             PT+ YFCFY A+ GNSQ++ NTVVLVTCVKNFPERRGV+LGLLKGF+G+G AVL Q++
Sbjct: 121 VRPTVEYFCFYFAVGGNSQTLANTVVLVTCVKNFPERRGVILGLLKGFLGLGGAVLTQVY 180

Query: 181 VAIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLS 240
            AIYG DTK+LIL IAW P+LI+L+F ++IRE+ +VKHPNEFRVF QFL +S+ILAL L+
Sbjct: 181 YAIYGHDTKSLILLIAWLPSLISLIFAYAIREVTIVKHPNEFRVFTQFLSVSLILALFLT 240

Query: 241 VLIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTP 300
           +LIF Q+ V FD L    VV AI+GLL+LPL I+IREE+VQWNL K+ K+ +  + PQ  
Sbjct: 241 ILIFLQREVRFDELTHVSVVTAIMGLLLLPLLIAIREELVQWNLNKITKLRKSLTLPQIS 300

Query: 301 LTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFT 360
           ++ AP       S Q +S+F NIF+KP+RGEDYT+LQAILSIDMLILY+TMIIGIGSSFT
Sbjct: 301 IS-APKLN--NPSFQTDSFFQNIFNKPKRGEDYTVLQAILSIDMLILYMTMIIGIGSSFT 360

Query: 361 AVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 420
           A+DN  QIGESQ Y  E++++ IS++SI NF GRIFSGF SEILLEK++FPRPLMLTFTL
Sbjct: 361 AMDNFGQIGESQGYSNESLDSIISIVSIFNFCGRIFSGFASEILLEKFKFPRPLMLTFTL 420

Query: 421 LVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSC 480
           L+SCIGH+LVAFPFH SL+IASI+ GFALGSQIPLY+A+ISEIFGLKHYS LYNFGQLSC
Sbjct: 421 LISCIGHILVAFPFHHSLYIASIITGFALGSQIPLYYAMISEIFGLKHYSFLYNFGQLSC 480

Query: 481 PIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILV 540
           PIGTYVLNVLVAG  YDQE  K   +SS   C G+ CYRN+F  LT ++L+GA ISLILV
Sbjct: 481 PIGTYVLNVLVAGKFYDQEA-KTSAYSSQFKCEGELCYRNSFVILTGISLLGAAISLILV 540

Query: 541 KRTAGFYRGDIYKKFREDMESLKTEVELYPLPVK 574
           +RT+ FYRGDIYKKFREDM+SLKT+++LY +  K
Sbjct: 541 RRTSEFYRGDIYKKFREDMDSLKTDMKLYAIDCK 570

BLAST of Lag0023014 vs. NCBI nr
Match: XP_008464809.1 (PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo])

HSP 1 Score: 732.6 bits (1890), Expect = 2.6e-207
Identity = 370/596 (62.08%), Postives = 470/596 (78.86%), Query Frame = 0

Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKL 60
           MIV+    +NGGIR GEL  F  QV++GRWF++F SFL+MT AGG YLF Y+S DI+  L
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  NCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVA 120
            CDQ++LN+IGFYKDLGSNVG++ GLL+EVA TW++L++ AAFNFIGYFK+WQ VTG++ 
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 NPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            PT+ +FCFY+ I  NSQ+  NT VLVTCVKNFPERRGV+LGLLKGFVG+  A++ QL++
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL IAWFP++++L+FV++IREIK VKHPNEFRVFVQFL ++++LALLL+ 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTR------IQS 300
           LIF QKRV FD  A   +V AI+ LL +PL I+IREE+V WNL K   ++       I+S
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 TPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGI 360
           +   P   +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI SIDMLI+  TM+IG+
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSFTAVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLM 420
           G+S TA+DNL QIGE+Q Y +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTFTLLVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNF 480
           LT  LL+S +GHLLVAFPF  SL++ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGI 540
           GQLSCPIG+Y+LNV+V G LYD EV ++G + +   C+G  CY  +F  L  LT + A I
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTK 591
           SLILV+RT  FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 595

BLAST of Lag0023014 vs. NCBI nr
Match: KAA0051898.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 729.6 bits (1882), Expect = 2.2e-206
Identity = 367/588 (62.41%), Postives = 466/588 (79.25%), Query Frame = 0

Query: 9   NNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLN 68
           +NGGIR GEL  F  QV++GRWF++F SFL+MT AGG YLF Y+S DI+  L CDQ++LN
Sbjct: 34  SNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTLN 93

Query: 69  KIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFC 128
           +IGFYKDLGSNVG++ GLL+EVA TW++L++ AAFNFIGYFK+WQ VTG++  PT+ +FC
Sbjct: 94  RIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFFC 153

Query: 129 FYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTK 188
           FY+ I  NSQ+  NT VLVTCVKNFPERRGV+LGLLKGFVG+  A++ QL++AIYG DTK
Sbjct: 154 FYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTK 213

Query: 189 TLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRV 248
           +LIL IAWFP++++L+FV++IREIK VKHPNEFRVFVQFL ++++LALLL+ LIF QKRV
Sbjct: 214 SLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKRV 273

Query: 249 EFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTR------IQSTPQTPLTL 308
            FD  A   +V AI+ LL +PL I+IREE+V WNL K   ++       I+S+   P   
Sbjct: 274 HFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPIH 333

Query: 309 APSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVD 368
           +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI SIDMLI+  TM+IG+G+S TA+D
Sbjct: 334 SPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAID 393

Query: 369 NLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVS 428
           NL QIGE+Q Y +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLMLT  LL+S
Sbjct: 394 NLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLIS 453

Query: 429 CIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIG 488
            +GHLLVAFPF  SL++ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NFGQLSCPIG
Sbjct: 454 SLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIG 513

Query: 489 TYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRT 548
           +Y+LNV+V G LYD EV ++G + +   C+G  CY  +F  L  LT + A ISLILV+RT
Sbjct: 514 SYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 573

Query: 549 AGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTK 591
             FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 574 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 620

BLAST of Lag0023014 vs. NCBI nr
Match: XP_023521066.1 (uncharacterized protein LOC111784665 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 728.0 bits (1878), Expect = 6.4e-206
Identity = 378/587 (64.40%), Postives = 473/587 (80.58%), Query Frame = 0

Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKL 60
           M+VST    +G I   E  SF RQVL GRWF++F SFL+MT AG FYL+ +FS DI+  L
Sbjct: 1   MVVSTIPIYHGRIETVEFSSFTRQVLGGRWFSLFASFLVMTSAGAFYLYSHFSKDIKETL 60

Query: 61  NCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVA 120
            CDQ++LNKIGFYKD+GSN+ I + LL E+A +W+LLV+ +A NFIGYFK+WQGV G++ 
Sbjct: 61  KCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWILLVICSAVNFIGYFKIWQGVVGKIV 120

Query: 121 NPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            P++ YFCFYMA+ GNSQ + N+VVLVTCV+NFPERRGV+LGLLKGF+G+GAAVL Q+++
Sbjct: 121 QPSVEYFCFYMAMGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYL 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL+IAWFP+LITL+F FSI+E+K+VKHP+EFRVFVQFL I++ILA+ L V
Sbjct: 181 AIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFVQFLCITLILAIFLIV 240

Query: 241 LIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPL 300
           L   Q+ VEFD  A   V+IAI+GLL+LPLFI+IREE+V WNL KM K  + Q++     
Sbjct: 241 LTIIQRIVEFDQSAHILVMIAIMGLLILPLFIAIREEMVHWNLNKMTKFEKCQTSAS--- 300

Query: 301 TLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTA 360
            ++ SS+ +Q S  RNS+ +NIF+KPERG+DYTILQ I SIDML++ LTM IGIGSS TA
Sbjct: 301 IISYSSSTLQHS--RNSYLSNIFNKPERGDDYTILQGIFSIDMLLICLTMSIGIGSSLTA 360

Query: 361 VDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLL 420
           +DNL QIGESQ Y  ++INA +S++SI NF GRIFSGFVSEILLE+Y+FPRPLMLT TLL
Sbjct: 361 MDNLGQIGESQGYSAQSINAIVSIISIFNFIGRIFSGFVSEILLEQYKFPRPLMLTLTLL 420

Query: 421 VSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCP 480
           VSCIG+LLVAFPF+ SL+IASI+IGFA GSQ+ L   IISEIFGLKHY+  YN  QLSCP
Sbjct: 421 VSCIGYLLVAFPFYNSLYIASILIGFAFGSQVLLDITIISEIFGLKHYAIFYNIAQLSCP 480

Query: 481 IGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVK 540
           IGTYVLNVLVAG LYD+EV K  + SS+  C G+QCYR++FT LT ++L+GA ISLILVK
Sbjct: 481 IGTYVLNVLVAGKLYDKEV-KTWIASSEYKCHGEQCYRDSFTILTGMSLLGAAISLILVK 540

Query: 541 RTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQST 588
           RT  FYRGDIY+KFREDM+SL+ EVELY L  ++  +  I + ++ T
Sbjct: 541 RTREFYRGDIYQKFREDMDSLREEVELYSLDERQNEAESIHVHKEVT 581

BLAST of Lag0023014 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.3e-207
Identity = 370/596 (62.08%), Postives = 470/596 (78.86%), Query Frame = 0

Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKL 60
           MIV+    +NGGIR GEL  F  QV++GRWF++F SFL+MT AGG YLF Y+S DI+  L
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  NCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVA 120
            CDQ++LN+IGFYKDLGSNVG++ GLL+EVA TW++L++ AAFNFIGYFK+WQ VTG++ 
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 NPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            PT+ +FCFY+ I  NSQ+  NT VLVTCVKNFPERRGV+LGLLKGFVG+  A++ QL++
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL IAWFP++++L+FV++IREIK VKHPNEFRVFVQFL ++++LALLL+ 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTR------IQS 300
           LIF QKRV FD  A   +V AI+ LL +PL I+IREE+V WNL K   ++       I+S
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 TPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGI 360
           +   P   +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI SIDMLI+  TM+IG+
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSFTAVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLM 420
           G+S TA+DNL QIGE+Q Y +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTFTLLVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNF 480
           LT  LL+S +GHLLVAFPF  SL++ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGI 540
           GQLSCPIG+Y+LNV+V G LYD EV ++G + +   C+G  CY  +F  L  LT + A I
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTK 591
           SLILV+RT  FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 595

BLAST of Lag0023014 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 1.1e-206
Identity = 367/588 (62.41%), Postives = 466/588 (79.25%), Query Frame = 0

Query: 9   NNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLN 68
           +NGGIR GEL  F  QV++GRWF++F SFL+MT AGG YLF Y+S DI+  L CDQ++LN
Sbjct: 34  SNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTLN 93

Query: 69  KIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFC 128
           +IGFYKDLGSNVG++ GLL+EVA TW++L++ AAFNFIGYFK+WQ VTG++  PT+ +FC
Sbjct: 94  RIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFFC 153

Query: 129 FYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTK 188
           FY+ I  NSQ+  NT VLVTCVKNFPERRGV+LGLLKGFVG+  A++ QL++AIYG DTK
Sbjct: 154 FYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTK 213

Query: 189 TLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRV 248
           +LIL IAWFP++++L+FV++IREIK VKHPNEFRVFVQFL ++++LALLL+ LIF QKRV
Sbjct: 214 SLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKRV 273

Query: 249 EFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTR------IQSTPQTPLTL 308
            FD  A   +V AI+ LL +PL I+IREE+V WNL K   ++       I+S+   P   
Sbjct: 274 HFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPIH 333

Query: 309 APSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVD 368
           +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI SIDMLI+  TM+IG+G+S TA+D
Sbjct: 334 SPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAID 393

Query: 369 NLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVS 428
           NL QIGE+Q Y +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLMLT  LL+S
Sbjct: 394 NLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLIS 453

Query: 429 CIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIG 488
            +GHLLVAFPF  SL++ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NFGQLSCPIG
Sbjct: 454 SLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIG 513

Query: 489 TYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRT 548
           +Y+LNV+V G LYD EV ++G + +   C+G  CY  +F  L  LT + A ISLILV+RT
Sbjct: 514 SYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 573

Query: 549 AGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQSTDTK 591
             FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 574 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 620

BLAST of Lag0023014 vs. ExPASy TrEMBL
Match: A0A6J1HLV5 (uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC111464764 PE=4 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 5.0e-204
Identity = 372/587 (63.37%), Postives = 472/587 (80.41%), Query Frame = 0

Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKL 60
           M+VST    +G I   E  SF RQVL GRWF++F SFL+MT AG FYL+ +FS DI+  L
Sbjct: 1   MVVSTIPIYHGRIGTVEFSSFTRQVLGGRWFSLFASFLVMTSAGAFYLYSHFSKDIKETL 60

Query: 61  NCDQSSLNKIGFYKDLGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVA 120
            CDQ++LNKIGFYKD+GSN+ I + LL E+A +WVLLV+ +A NFIGYFK+WQGV G++ 
Sbjct: 61  QCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVICSAVNFIGYFKIWQGVVGKIV 120

Query: 121 NPTLGYFCFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            P++ YFCFY+A+ GNSQ + N+VVLVTCV+NFPERRGV+LGLLKGF+G+GAAVL Q+++
Sbjct: 121 QPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYL 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL+IAWFP+LITL+F FSI+E+K+VKHP+EFRVF+QFL I++ILA+ + V
Sbjct: 181 AIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIV 240

Query: 241 LIFAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPL 300
           L   Q+ V+FD  A   V+IAI+GLL+LPLFI+IREE+V+WNL KM K  + Q++     
Sbjct: 241 LTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSAS--- 300

Query: 301 TLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTA 360
            ++ SS+ +Q S  RNS  +NIF+KPERG+DYTILQ   SIDML++ LTM IGIGSS TA
Sbjct: 301 IISYSSSTLQHS--RNSDLSNIFNKPERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTA 360

Query: 361 VDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLL 420
           +DNL QIGESQ Y  E+INA ++++SI NF GRIFSGFVSEILLE+Y+FPRPLMLT TLL
Sbjct: 361 MDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLL 420

Query: 421 VSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCP 480
           VSCIG+LLVAFPF+ SL+IASI+IGFA GSQ+ L   +ISEIFGLKHY+  YN  QLSCP
Sbjct: 421 VSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCP 480

Query: 481 IGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVK 540
           IGTYVLNVLVAG LYD+EV K  + SS+  C G+QCY+++FT LT ++L+GA ISLILVK
Sbjct: 481 IGTYVLNVLVAGKLYDKEV-KTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVK 540

Query: 541 RTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPPSSVITIDEQST 588
           RT  FYRGDIY+KFREDM+SL+ EVELY L V++     + + ++ T
Sbjct: 541 RTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKEVT 581

BLAST of Lag0023014 vs. ExPASy TrEMBL
Match: A0A6J1I3Q6 (uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393 PE=4 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 3.0e-201
Identity = 363/571 (63.57%), Postives = 455/571 (79.68%), Query Frame = 0

Query: 17  ELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDL 76
           +  +FARQV++GRWF++F SFL+MT AG FY + YFS  I+  L CDQ++LNKI FYKD+
Sbjct: 5   QFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDI 64

Query: 77  GSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAGN 136
           GSN+ IV+ LL  +A +WVLL++ +A NF+GYFK+WQ V GQV +PT+ YF  Y+A+AGN
Sbjct: 65  GSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGN 124

Query: 137 SQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAW 196
           SQ + NTVVLV CV+NFPERRGV+LGLLKGF+G+GAAVL Q+++AIYG DTK+LIL+IAW
Sbjct: 125 SQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAW 184

Query: 197 FPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDALADT 256
            P+LITL+F FSIRE+K+VKHP+EFRVF+Q L I+++LAL L+VL  A++ V+FD LA T
Sbjct: 185 CPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYT 244

Query: 257 FVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPLTLAPSSTKVQQSKQRN 316
           FVV+AI+GLL+LPLFI+IREE+VQ NL  M K  + +S PQ P+               N
Sbjct: 245 FVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAIS-----HNQNHGN 304

Query: 317 SWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQIGESQRYKTE 376
           S+++NIF+KPERG+DYTILQ I SIDML++ L M IG GSS TA+DNL QIGESQ Y TE
Sbjct: 305 SYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTE 364

Query: 377 TINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGS 436
           ++NA +S++SI NF GRIFSGFVSEILLEKY+FPRPLMLT TLLVSCIG LLVAFPFH S
Sbjct: 365 SVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNS 424

Query: 437 LFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLNVLVAGNLYD 496
           L+IASI+IGFA GSQ+ L   +ISE FGL+HY+  YN  QL+CPIGTYVL VLVAG LYD
Sbjct: 425 LYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYD 484

Query: 497 QEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYRGDIYKKFRE 556
           +EV K    S + IC G+QCYRN+FT+LT ++L+GA ISLILVKRT  FYR DIYKKFRE
Sbjct: 485 EEV-KTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFRE 544

Query: 557 DMESLKTEVELYPLPVKRPPSSVITIDEQST 588
           DM+SLK EVELYP  V++P    + + ++ T
Sbjct: 545 DMDSLKEEVELYPHDVRQPEVESVHVHKEVT 569

BLAST of Lag0023014 vs. ExPASy TrEMBL
Match: A0A6J1EYR7 (uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC111440576 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 1.1e-200
Identity = 362/574 (63.07%), Postives = 455/574 (79.27%), Query Frame = 0

Query: 17  ELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDL 76
           +  +FARQV++GRWF++F SFL+MT AG FYL+ YF   I+  L CDQ++LNKI FYKD+
Sbjct: 5   QFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYAYFPEHIKETLQCDQTTLNKISFYKDI 64

Query: 77  GSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAGN 136
           GSN+ +V+ LL  +A +W+LL++ +A NFIGYFK+WQ V GQ+A+PT+ YF  Y+AIAGN
Sbjct: 65  GSNIDVVSALLVYIAPSWILLLICSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIAIAGN 124

Query: 137 SQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAW 196
           SQ + NTVVLV CV+NFPERRG++LGLLKGF+G+GAAVL Q+++AIYG DTK+LIL+IAW
Sbjct: 125 SQILANTVVLVICVRNFPERRGMILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAW 184

Query: 197 FPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDALADT 256
            P+LITL F FSIRE+K+VKHP+EFRVF+Q L I+++LAL L+VL  A++ V+FD LA T
Sbjct: 185 CPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYT 244

Query: 257 FVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPLTLAPSSTKVQQSKQRN 316
           FVV+AI+GLL+LPLFI+IREE+VQ NL  M K  + QS PQ P+               N
Sbjct: 245 FVVVAIIGLLILPLFIAIREEMVQLNLNNMIKFEKSQSKPQRPIHAIS-----HNQNHGN 304

Query: 317 SWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQIGESQRYKTE 376
           S ++NIF+KPERG+DYTILQ   SIDML++ LT+ IG GSS TA+DNL QIGESQ Y TE
Sbjct: 305 SHYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNLGQIGESQGYSTE 364

Query: 377 TINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGS 436
           ++NA +S++SI NF GRIFSGFVSEILLEKY+FPRPLMLT TLLVSCIG LLVAFPFH S
Sbjct: 365 SVNAMVSIISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNS 424

Query: 437 LFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLNVLVAGNLYD 496
           L+IASI+IGF+ GSQ+ L   +ISEIFGL+HY+  YN  QLSCPIGTYVL VLVAG LYD
Sbjct: 425 LYIASILIGFSFGSQVLLDITMISEIFGLEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYD 484

Query: 497 QEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYRGDIYKKFRE 556
           + V K     ++ IC G+QCYR++FT+LT ++L+GA ISLILVKRT  FYR DIYKKFRE
Sbjct: 485 EAV-KTWSDPTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREFYREDIYKKFRE 544

Query: 557 DMESLKTEVELYPLPVKRPPSSVITIDEQSTDTK 591
           DM+SLK EVELYP  V++P    I + ++ T  K
Sbjct: 545 DMDSLKEEVELYPHDVRQPEVESIHVHQEGTIIK 572

BLAST of Lag0023014 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 538.5 bits (1386), Expect = 6.8e-153
Identity = 291/554 (52.53%), Postives = 385/554 (69.49%), Query Frame = 0

Query: 28  GRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDLGSNVGIVTGLL 87
           GRWF +F SFLIM  AG  YLFG +S DI++ L  DQ++LN +GF+KDLG+NVG+++GL+
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 88  SEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAGNSQSIVNTVVLV 147
           +EV  TW +L + +A NF+GYF +W  VTG+VA P +   C Y+ I  NSQ+  NT  LV
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 148 TCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAWFPTLITLMFVF 207
           TCVKNFPE RGV+LGLLKG+VG+  A+  QL+ AIYG D+K+LIL IAW P  ++L+FV+
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVY 195

Query: 208 SIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDALADTFVVIAIVGLLV 267
            IRE KVV+  NE  VF QFL+ISI LAL L  +  A+K+V F   A          LL 
Sbjct: 196 LIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLF 255

Query: 268 LPLFISIREEIVQWNLKKM--AKVTRIQ-STPQTPLTL-APSSTKVQ-QSKQRNSWFANI 327
           +PL +S+++E+  WN+ K+   + + ++   P+  L L    + KV  + K+  S F+ +
Sbjct: 256 VPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTV 315

Query: 328 FDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQIGESQRYKTETINAFI 387
           F  P RGEDYTILQA+LS DM+IL++    G+GSS TAVDNL QIGES  Y   T+++F+
Sbjct: 316 FSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFV 375

Query: 388 SVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGSLFIASI 447
           S++SI N+ GR+FSGFVSE LL KY+ PRPLM+T  LL+SC GHLL+AFP  GS++IASI
Sbjct: 376 SLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASI 435

Query: 448 VIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLNVLVAGNLYDQEVEKM 507
           ++GF+ G+Q+PL FAIISE+FGLK+YS L+N GQL+ P+G+Y+LNV V G LYD+E  K 
Sbjct: 436 LMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQ 495

Query: 508 ----GLHSSDG---ICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYRGDIYKKFR 567
               GL   D     C+G QCY+  F  L A+T  GA +SL L  RT  FY+GDIYKKFR
Sbjct: 496 LTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFR 555

Query: 568 EDMESLKTEVELYP 570
           E  ES   E EL P
Sbjct: 556 ESPES---ESELVP 566

BLAST of Lag0023014 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.9e-139
Identity = 267/569 (46.92%), Postives = 369/569 (64.85%), Query Frame = 0

Query: 16  GELPSFARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKD 75
           G + S   Q+L GRWF  F S LIM+ AG  Y+FG +S DI+  L  DQ++LN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGIVTGLLSEVASTWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAG 135
           LG+NVG++ GLL+EV   W +L++ A  NF GYF +W  VT +++ P + + C Y+ +  
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIA 195
           NSQS  NT  LVTCVKNFPE RGVVLG+LKG+VG+  A++ QL+ A YG DTK LIL I 
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDALAD 255
           W P +++  F+ +IR +KV +  NE +VF  FL+IS+ LA  L V+I   K   F     
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 TFVVIAIVGLLVLPLFISIREEIVQWNLKKM-----AKVTRIQSTPQTPLTL-----APS 315
                 ++ LL+LP+ + I EE   W  K++     A +  +   P+   +         
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEE 307

Query: 316 STKVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLT 375
           S +V +  +  S +  +F+ PERG+DYTILQA+ S+DMLIL+L  I G+G + TA+DNL 
Sbjct: 308 SKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLG 367

Query: 376 QIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIG 435
           QIG S  Y   +++ F+S++SI N+ GR+ SG VSEI L KY+FPRPLMLT  LL+SC G
Sbjct: 368 QIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAG 427

Query: 436 HLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYV 495
           HLL+AF   G L++AS++IGF  G+Q PL FAIISEIFGLK+YS LYNFG ++ PIG+Y+
Sbjct: 428 HLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYL 487

Query: 496 LNVLVAGNLYDQE-------VEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLIL 555
           LNV VAG LYD E       + K  +   D  CIG  C++ +F  + A+TL G  +S++L
Sbjct: 488 LNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVL 547

Query: 556 VKRTAGFYRGDIYKKFREDMESLKTEVEL 568
           V RT  FY+ DIYKKFRE  ++L  E+E+
Sbjct: 548 VIRTKKFYKSDIYKKFRE--KALAAEMEM 574

BLAST of Lag0023014 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.4e-73
Identity = 173/546 (31.68%), Postives = 299/546 (54.76%), Query Frame = 0

Query: 22  ARQVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDLGSNVG 81
           A ++L  +W A+  S  I   AGG Y FG +S  +++  + DQS+L+ +  +KD+G NVG
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 82  IVTGLLSEVAS--------------TWVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYF 141
           +++GL+   A+               WV++++ A  NF GYF +W  VTG +  P +   
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 142 CFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDT 201
           C +M IA  S + +NT  +V+ ++NF +  G  +G++KGFVG+  A+LIQL+  +   D 
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 202 KTLILWIAWFPTLITLMFVFSIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKR 261
           KT IL +A  P+L++++ +  +R  K     +E +       +S+I+A  L + I  +  
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 241

Query: 262 VEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPLTLAPSST 321
           +   + A+   +  ++ LL  PL +++R                 + + + PL+ +  S 
Sbjct: 242 LSLPSWANAVTLAVLLVLLSSPLLVAVRAH---------------RDSIEKPLS-SVYSP 301

Query: 322 KVQQSKQRNSWFANIFDKPERGEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQI 381
            V   +   S    + D+    +   +LQA+ ++D  +L+L MI G+GS  + ++N+ QI
Sbjct: 302 LVDNLEATTSGEILMLDE---DKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQI 361

Query: 382 GESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHL 441
           GES RY +  IN+ +++ +I NF GR   G+VS+ LL +  +PRPL++  TL    IGHL
Sbjct: 362 GESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHL 421

Query: 442 LVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLN 501
           ++A  F G+L+  SI++G   GSQ  L   I SE+FG+KH   +YN   ++ P+G+Y+ +
Sbjct: 422 IIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFS 481

Query: 502 VLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYR 554
           V + G +YD+ +   G       C G  C+R A+  + ++  +G  +S +LV RT   YR
Sbjct: 482 VRLIGYIYDRTIIGEG-----NTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR 521

BLAST of Lag0023014 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 4.1e-73
Identity = 175/554 (31.59%), Postives = 298/554 (53.79%), Query Frame = 0

Query: 24  QVLLGRWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDLGSNVGIV 83
           ++L  +W A+  S  I   +G  Y FG +S  +++  + DQS+L+ +  +KD+G+N G+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  TGLLSEVAST----------------WVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYF 143
           +GLL   A++                WV+L V A   F GYF +W  VTG +  P +   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CFYMAIAGNSQSIVNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDT 203
           C +M +A  SQ+  NT  +V+ V+NF +  G  +G++KGF+G+  A+LIQL+  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 KTLILWIAWFPTLITLMFVFSIR--EIKVV---KHPNEFRVFVQFLWISIILALLLSVLI 263
            + IL +A  PT+++L+ +  +R  E  V    KH N          +S+I+A  L ++I
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSA------VSLIIAAYLMIII 241

Query: 264 FAQKRVEFDALADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTPQTPLTL 323
             +      + A+   ++ ++ +L LPL I+ R +           + +      +PL  
Sbjct: 242 ILKNTFGLSSWANIVTLVCLLVMLALPLLIARRAQ--------RDGMEKTVPHDYSPLIS 301

Query: 324 AP-SSTKVQQSKQRNSWFANIFDKPERG--EDYTILQAILSIDMLILYLTMIIGIGSSFT 383
           +P ++T   QS + +S       K E G  E+  +LQA+  +   +L+L MI G+GS  +
Sbjct: 302 SPKATTSGNQSSEGDS-------KVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLS 361

Query: 384 AVDNLTQIGESQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 443
            ++N+ QIGES RY +  IN+ +S+ SI NF GR  +G+ S+ LL K  +PRPL++  TL
Sbjct: 362 TINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATL 421

Query: 444 LVSCIGHLLVAFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSC 503
               IGHL++A  F G+L++ S+++G   GSQ  L   I SE+FG++H   ++N   ++ 
Sbjct: 422 GTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVAS 481

Query: 504 PIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILV 554
           PIG+Y+ +V + G +YD+     G       C G  C+R +F  + ++   G  ++++L 
Sbjct: 482 PIGSYIFSVRLIGYIYDKTASGEG-----NTCYGSHCFRLSFIIMASVAFFGFLVAIVLF 529

BLAST of Lag0023014 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 7.6e-72
Identity = 176/544 (32.35%), Postives = 290/544 (53.31%), Query Frame = 0

Query: 29  RWFAIFVSFLIMTFAGGFYLFGYFSNDIRAKLNCDQSSLNKIGFYKDLGSNVGIVTGLLS 88
           +W A   S  I +F+G  Y F  +S+ +++  + DQS+L+ +  +KD+G   GI++G L 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 89  EVAST--------WVLLVVYAAFNFIGYFKVWQGVTGQVANPTLGYFCFYMAIAGNSQSI 148
              ++        WV++ V     F+G+F +W  V G +A P +   C ++ +AG+S   
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 149 VNTVVLVTCVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRD--TKTLILWIAWFP 208
            NT  +VT  +NF +  G  +G+++GF+G+  A+LIQL+ A+ G +    T IL +A  P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 209 TLITLMFVFSIREIKVV-----KHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAL 268
           TL+  + +  +R  + V     KH +          IS+I+A  L V+I  +  +     
Sbjct: 187 TLVMFLAMPFVRVYETVTISDKKHLDGLSA------ISMIIAAYLMVVITVENVLGLSRS 246

Query: 269 ADTFVVIAIVGLLVLPLFISIREEIVQWNLKKMAKVTRIQSTP--QTPLTLAPSSTKVQQ 328
              F  I ++ LL  PL +++R       L++  +       P   T   L P S     
Sbjct: 247 MQIFSFILVLLLLASPLLVAVRA------LREKRQTLSSLDGPVLDTSALLDPPS----- 306

Query: 329 SKQRNSWFANIFDKPER--GEDYTILQAILSIDMLILYLTMIIGIGSSFTAVDNLTQIGE 388
                   +NIF   +    ED  IL+A+ +++  +L+L M+ G+GS F  V+N+ QIGE
Sbjct: 307 --------SNIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGE 366

Query: 389 SQRYKTETINAFISVLSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLV 448
           S RY +  +N+ +S+ SI NF GR  +G+VS+  L K+ +PRP+ +  TL V  IGH++V
Sbjct: 367 SLRYSSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIV 426

Query: 449 AFPFHGSLFIASIVIGFALGSQIPLYFAIISEIFGLKHYSQLYNFGQLSCPIGTYVLNVL 508
           A    GSL+  S++IG A GSQ  L   I SEIFG++H   +Y    ++ PIG+Y+L+V 
Sbjct: 427 ASGVQGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVK 486

Query: 509 VAGNLYDQEVEKMGLHSSDGICIGDQCYRNAFTYLTALTLVGAGISLILVKRTAGFYRGD 554
           V G  YD+   +      D  C G QC+R +F  + ++ L G+ ++ +L  RT  FY+  
Sbjct: 487 VIGYFYDKVASE-----DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNL 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147304.16.6e-21967.06uncharacterized protein LOC101203173 [Cucumis sativus] >KAE8652743.1 hypothetica... [more]
XP_038895749.11.2e-21266.72uncharacterized protein LOC120083913 [Benincasa hispida][more]
XP_008464809.12.6e-20762.08PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo][more]
KAA0051898.12.2e-20662.41protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protei... [more]
XP_023521066.16.4e-20664.40uncharacterized protein LOC111784665 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3CMG91.3e-20762.08uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=... [more]
A0A5D3BP481.1e-20662.41Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1HLV55.0e-20463.37uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I3Q63.0e-20163.57uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393... [more]
A0A6J1EYR71.1e-20063.07uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC1114405... [more]
Match NameE-valueIdentityDescription
AT2G28120.16.8e-15352.53Major facilitator superfamily protein [more]
AT2G39210.11.9e-13946.92Major facilitator superfamily protein [more]
AT1G18940.11.4e-7331.68Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G74780.14.1e-7331.59Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.17.6e-7232.35Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..274
e-value: 1.0E-65
score: 221.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 318..546
e-value: 2.0E-12
score: 48.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 227..545
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 30..240
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 14..562
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 14..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0023014.1Lag0023014.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane