Lag0022679 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0022679
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Histidine--tRNA ligase
Location	chr7: 35456191 .. 35457825 (+)
RNA-Seq Expression	Lag0022679
Synteny	Lag0022679

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTGAAATTGAAATGTTGAATAAATTGGAAAGGGAAAGAAAACAGAGCAGAGATAGAAGAAGAAAGGCCAGAGGAGAAAAGTTCAATCTAGAAGGTTCCTTTAGTGGAAGAGTGAGAGGTTTTAAATGGGAGAAGACTTACCATACCACTCTCTATTTCGTACCATTTGGTTTTGTGCCATCTCTTTACTGAAGTGGTATGTCCCCACTCTCTATTTGTATTTTCTTTTGTTCTTTACCTTCTACTTATTTTTTCTATTTTCTTTTGTTGGTTAAATTGTAAACATCTTCGAAAGTTGCAGCTTCTTCCTTGGATTTTCTGGTGGAATGTTTTCTTTTTTTATAGGATTATTTTATTTGATTTGCAATGCAACTTCCTTGGTTGTTGTTGAAGTTGGGTGGTTTGGGTGGTTGGTGTTGGAGTTGGTCTTCACCACTTTGTTTCCTAAACCAGTTGGACCTTGGAGAACGATTTGCAGTGGTTATTAGAGTATCTTGTTTGTTTTACAACACTATTTTATTATCTTCAATGCATTTTACAACACTGTTTTATTATCTTCAATGCACAAATGAAGGGGAAGTGAAGGTAAGAAGGAGAAATCACAATTTTGGTTTTAAGGTTTTCCTTTTTGGGAGTTGAAGTAGAATAACATCCTATGACTCTTAGTTTACCATCGATCTCCAAGTTGACGTCTAGAACAAGATTTTGGATGTTATTTATACCACTTGACATGCTTCCAGAATGTAGCTTCTGTTTCTAGAACAAGATTCTGTCTAGCCATTCGATAAGTGTGCTTTTGCGACTTCTTTGTTTGGCTTTTATTTTGTTGTGTATATGGATGTTGATTGATGGAAGATGGTAGCTATTAAGCATTTGAAGCAAAACTTCTATCTTCTATTGTTAACTTTTTCAAGCGAATTGGAATTACAGTCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTATTTTTTTCTGCATCTTGTGTGATCTCCAATAAGTTTAGTTATTGTTTTAGTAGTTATTACACATTCAATCGTGAATCAAAAAGTTTCATAGTTATAATTTGTGTAAATGCCTTATGCTCTTGGTTTGATGGTAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATATTCATAGACAAGGTTAGTGATCTACAATTTCAAGTTCCATAAAGTTTTATATTTTTGTGCCTTTTCCCTACACATGTCTGAATGTAATAATTACTACTTTCTTTGTTTCCTTTGGTTGTTAATATTAATAACATATAGAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGTTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGGTTGTTTTCATAATGCTTTAGTCTTTGACAAATTAGTTGGAATGCATGTGATAACTCCACTGAGGTTGAAGTGTGATTGTTATTCTTGCATAGAAATACTTGGAGGTGGTGGCGAAGCATTGTCTGATCTGAAGCAGCTATTTTCTCTTACTGAAAAGTTTGGGTATGTTGATTGGGTCAAATTTGATGCATCCTTTGTCTGTGGCCTTGCTTAA

mRNA sequence

ATGTTTGAAATTGAAATGTTGAATAAATTGGAAAGGGAAAGAAAACAGAGCAGAGATAGAAGAAGAAAGGCCAGAGGAGAAAAGTTCAATCTAGAAGGATTATTTTATTTGATTTGCAATGCAACTTCCTTGGTTGTTGTTGAAGTTGGGTGGTTTGGGTGGTTGGTGTTGGAGTTGGTCTTCACCACTTTGTTTCCTAAACCAGTTGGACCTTGGAGAACGATTTGCAGTGTCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATATTCATAGACAAGGTTAAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGTTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATACTTGGAGGTGGTGGCGAAGCATTGTCTGATCTGAAGCAGCTATTTTCTCTTACTGAAAAGTTTGGGTATGTTGATTGGGTCAAATTTGATGCATCCTTTGTCTGTGGCCTTGCTTAA

Coding sequence (CDS)

ATGTTTGAAATTGAAATGTTGAATAAATTGGAAAGGGAAAGAAAACAGAGCAGAGATAGAAGAAGAAAGGCCAGAGGAGAAAAGTTCAATCTAGAAGGATTATTTTATTTGATTTGCAATGCAACTTCCTTGGTTGTTGTTGAAGTTGGGTGGTTTGGGTGGTTGGTGTTGGAGTTGGTCTTCACCACTTTGTTTCCTAAACCAGTTGGACCTTGGAGAACGATTTGCAGTGTCCCAGATGTTGGATTCAAGGTTTCCAGTCGGAAGGTTTTGCAAGAAGTATTAAAATGCTATGGAATACCTGAAAATCTGTTTGGCAAGGTTTGCATATTCATAGACAAGGTTAAGAAAATTCCATTAGATGAGATGAAGAAGGAACTGAAGGTCGTGGGTGTATCAGAGGAGGCTATTGAGGAGTTGCTGAAAGTTCTTTCAGTAAAGTTTTTGACAAAGTTAGAAGAAATACTTGGAGGTGGTGGCGAAGCATTGTCTGATCTGAAGCAGCTATTTTCTCTTACTGAAAAGTTTGGGTATGTTGATTGGGTCAAATTTGATGCATCCTTTGTCTGTGGCCTTGCTTAA

Protein sequence

MFEIEMLNKLERERKQSRDRRRKARGEKFNLEGLFYLICNATSLVVVEVGWFGWLVLELVFTTLFPKPVGPWRTICSVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEELLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA

Homology

[Close]

BLAST of Lag0022679 vs. NCBI nr
Match: KAG6604395.1 (Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 199.5 bits (506), Expect = 2.6e-47
Identity = 99/114 (86.84%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK++KIPLDEMKKELKVVGVSE+AIEE
Sbjct: 243 DVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEE 302

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           +L+VLSVK LTKLEEILGGGGEA+SDLKQLFSL EKFGY DW++FDAS V GLA
Sbjct: 303 MLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLA 356

[Close]

BLAST of Lag0022679 vs. NCBI nr
Match: XP_023544233.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.5 bits (506), Expect = 2.6e-47
Identity = 99/114 (86.84%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK++KIPLDEMKKELKVVGVSE+AIEE
Sbjct: 243 DVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEE 302

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           +L+VLSVK LTKLEEILGGGGEA+SDLKQLFSL EKFGY DW++FDAS V GLA
Sbjct: 303 MLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLA 356

[Close]

BLAST of Lag0022679 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 199.5 bits (506), Expect = 2.6e-47
Identity = 99/114 (86.84%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK++KIPLDEMKKELKVVGVSE+AIEE
Sbjct: 243 DVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEE 302

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           +L+VLSVK LTKLEEILGGGGEA+SDLKQLFSL EKFGY DW++FDAS V GLA
Sbjct: 303 MLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLA 356

[Close]

BLAST of Lag0022679 vs. NCBI nr
Match: XP_023544232.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.5 bits (506), Expect = 2.6e-47
Identity = 99/114 (86.84%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK++KIPLDEMKKELKVVGVSE+AIEE
Sbjct: 243 DVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEE 302

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           +L+VLSVK LTKLEEILGGGGEA+SDLKQLFSL EKFGY DW++FDAS V GLA
Sbjct: 303 MLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLA 356

[Close]

BLAST of Lag0022679 vs. NCBI nr
Match: KAG7034545.1 (Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 199.5 bits (506), Expect = 2.6e-47
Identity = 99/114 (86.84%), Postives = 108/114 (94.74%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQEVLKCYGIPEN+FGKVCI IDK++KIPLDEMKKELKVVGVSE+AIEE
Sbjct: 125 DVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEE 184

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           +L+VLSVK LTKLEEILGGGGEA+SDLKQLFSL EKFGY DW++FDAS V GLA
Sbjct: 185 MLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLA 238

[Close]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot
Match: O82413 (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.9e-41
Identity = 82/114 (71.93%), Postives = 101/114 (88.60%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAIEE 139
           DVGFKVSSRKVLQE+LK YG+PE+LFG+VCI IDK++KIP+DE+KKEL   G+SE+AIE+
Sbjct: 222 DVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKELGFTGISEDAIEQ 281

Query: 140 LLKVLSVKFLTKLEEILGGGGEALSDLKQLFSLTEKFGYVDWVKFDASFVCGLA 194
           LL+VLSVK L  LE+I+GG GEA++DLKQLFSL EKFGY +W++FDAS V GLA
Sbjct: 282 LLQVLSVKSLDDLEDIIGGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLA 335

[Close]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot
Match: P70076 (Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-07
Identity = 38/123 (30.89%), Postives = 62/123 (50.41%), Query Frame = 0


Query: 80  DVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKEL-KVVGVSEEAIE 139
           D   KV+ R++L  +    G+P+N+F  +C  +DK+ K+P + +K E+    G+SEEA +
Sbjct: 211 DFRIKVNDRRILDGMFAVCGVPDNMFRTICSTVDKLDKLPWEAVKNEMVNEKGLSEEAAD 270

Query: 140 ELLKVLSVKFLTKLEEIL---------GGGGEALSDLKQLFSLTEKFGYVDWVKFDASFV 193
           ++   + ++    L E L               L+D+K LFS  + F   D V FD S  
Sbjct: 271 QIGVYVGMQGGMDLAERLLQDQKMCQSTQACAGLTDIKLLFSYLQLFQVTDKVVFDLSLA 330

[Close]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot
Match: Q86AS6 (Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.7e-07
Identity = 39/126 (30.95%), Postives = 64/126 (50.79%), Query Frame = 0


Query: 78  VPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKVVGVSEEAI 137
           V D   K++ RK+L  +    G+P + F  +C  +DK+ K P +E++KE+  V   + A+
Sbjct: 187 VGDFQIKLNHRKLLDAIFAICGVPADKFRAICSAVDKLDKSPWEEVRKEMVEVKALDGAV 246

Query: 138 -EELLKVLSVKF--LTKLEEILGGG--------GEALSDLKQLFSLTEKFGYVDWVKFDA 193
            +++ K +S+K   +  L+ +   G         EALS L+ LF   E FG    + FD 
Sbjct: 247 ADKIEKFVSLKDEPIKLLQHLRATGMCDGNKDATEALSQLETLFGYLECFGVTQHILFDL 306

[Close]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot
Match: E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 8.0e-07
Identity = 43/146 (29.45%), Postives = 69/146 (47.26%), Query Frame = 0


Query: 57  LELVFTTLFPKPVGPWRTICSVPDVGFKVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVK 116
           L+LV+  L    +G +R          KV+ R++L  +    G+P+  F  +C  +DK+ 
Sbjct: 192 LKLVYEILSELDLGDFR---------IKVNDRRILDGMFAICGVPDEKFRTICSTVDKLD 251

Query: 117 KIPLDEMKKEL-KVVGVSEEAIEELLKVLSVKFLTKLEEIL---------GGGGEALSDL 176
           K+  +E+KKE+    G+SEE  + +   +S++    L E L               ++D+
Sbjct: 252 KLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGKDLAERLLQDPKLSQSKQACAGITDM 311

Query: 177 KQLFSLTEKFGYVDWVKFDASFVCGL 193
           K LFS  E F   D V FD S   GL
Sbjct: 312 KLLFSYLELFQITDKVVFDLSLARGL 328

[Close]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot
Match: O43011 (Histidine--tRNA ligase, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hts1 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 4.0e-06
Identity = 34/119 (28.57%), Postives = 64/119 (53.78%), Query Frame = 0


Query: 84  KVSSRKVLQEVLKCYGIPENLFGKVCIFIDKVKKIPLDEMKKELKV-VGVSEEAIEELLK 143
           K++ RK+L  +    G+P++    +   +DK+ K+P +++++E+ V  G+ EE  +++ +
Sbjct: 243 KLNHRKILDGIFTVCGVPDDKIRTISSAVDKLDKLPWEDVRREMVVEKGLKEEVADKIKE 302

Query: 144 VLSVK----FLTKLE--EILGGGGEAL---SDLKQLFSLTEKFGYVDWVKFDASFVCGL 193
            + +K     L KLE   +L     A+   +D++ LF   E FG +D   FD S   GL
Sbjct: 303 YVLLKGDRSLLDKLEADSLLSSNSSAVAAFNDMRLLFDYLEAFGVLDRFSFDMSLARGL 361

The following BLAST results are available for this feature:

BLAST of Lag0022679 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6604395.1	2.6e-47	86.84	Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]
XP_023544233.1	2.6e-47	86.84	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo s...	[more]
XP_022978407.1	2.6e-47	86.84	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima...	[more]
XP_023544232.1	2.6e-47	86.84	histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo s...	[more]
KAG7034545.1	2.6e-47	86.84	Histidine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]

BLAST of Lag0022679 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82413	2.9e-41	71.93	Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
P70076	2.1e-07	30.89	Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 ...	[more]
Q86AS6	4.7e-07	30.95	Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS...	[more]
E9QI36	8.0e-07	29.45	Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2	[more]
O43011	4.0e-06	28.57	Histidine--tRNA ligase, mitochondrial OS=Schizosaccharomyces pombe (strain 972 /...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 7..27
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 73..193 e-value: 1.1E-20 score: 75.9
None	No IPR available	PANTHER	PTHR43707:SF1	HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED	coord: 79..193
IPR004516	Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit	PANTHER	PTHR43707	HISTIDYL-TRNA SYNTHETASE	coord: 79..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0022679.1	Lag0022679.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006427	histidyl-tRNA aminoacylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004821	histidine-tRNA ligase activity